Standard name
Human Ortholog
Description RNA polymerase II third largest subunit B44; part of central core; similar to prokaryotic alpha subunit
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0.05 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Cytoplasm 0.05 0 0.07 0 0 0 0 0 0.05 0.05 0.05 0 0 0.1 0.07 – – – 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0.05 0 0.07 0 0 0 0 – – – 0 0 0 0 0 0
Nucleus 0.75 0.71 0.67 0.81 0.79 0.8 0.68 0.69 0.65 0.7 0.62 0.66 0.75 0.66 0.69 – – – 0.59 0.59 0.43 0.5 0.44 0.48
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Nucleolus 0.7 0.76 0.62 0.37 0.29 0.31 0.48 0.51 0.57 0.54 0.69 0.62 0.49 0.32 0.35 – – – 0.32 0.36 0.5 0.35 0.45 0.41
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0.06 0.06
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Bud 0 0 0 0 0 0 1 1 0 1 0 0 0 0 0 – – – 0 1 0 3 1 4
Bud Neck 0 1 0 0 0 0 1 1 1 2 1 0 1 0 0 – – – 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 4 0 8 4 6 1 0 2 0 – – – – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Cytoplasm 1 2 4 2 10 6 8 11 12 14 10 5 6 19 16 – – – 1 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 1 0 0 0
Endosome 0 1 0 0 1 1 1 2 0 0 0 0 1 3 2 – – – 1 0 0 0 1 1
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 – – – 0 0 0 0 0 0
Mitochondria 0 1 0 0 1 1 9 3 12 7 16 5 1 0 0 – – – 0 0 0 0 2 1
Nucleus 15 119 41 107 181 173 212 182 164 197 135 134 131 123 150 – – – 33 104 41 38 121 110
Nuclear Periphery 0 0 0 0 0 0 2 1 1 0 0 0 0 0 0 – – – 0 0 0 0 1 0
Nucleolus 14 127 38 49 66 66 152 135 142 150 151 126 86 60 75 – – – 18 62 48 27 124 93
Peroxisomes 0 1 0 0 0 0 1 0 0 0 0 0 0 0 4 – – – 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 2 1 0 0 0 0 1 5 4 – – – 0 1 0 1 0 1
Vac/Vac Membrane 0 4 1 2 6 1 0 1 1 1 1 1 1 3 7 – – – 0 4 0 3 15 12
Unique Cell Count 20 168 61 132 229 216 314 264 251 280 219 203 175 187 217 57 176 97 77 276 228
Labelled Cell Count 30 256 84 160 265 248 393 338 341 376 320 272 228 215 259 57 176 97 77 276 228


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 15.1 26.1 18.1 16.8 16.5 14.1 13.0 13.6 12.3 12.4 11.3 12.4 18.7 18.9 19.6 – – – 17.4 20.6 22.4
Std Deviation (1e-4) 4.8 6.2 5.5 4.3 4.0 3.6 3.7 3.6 3.7 4.0 3.2 3.5 5.8 6.5 7.1 – – – 6.2 5.7 7.2
Intensity Change (Log2) – – – -0.1 -0.14 -0.36 -0.47 -0.42 -0.56 -0.55 -0.68 -0.54 0.05 0.06 0.11 – – – -0.06 0.18 0.31


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Bud 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Cytoplasm 0 0 0 0 0 0 0 0 0 0 0.8 0 – – –
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Nucleus 2.1 1.9 2.1 0 0.3 -0.3 0.5 -0.8 -0.2 1.2 -0.2 0.3 – – –
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Nucleolus -3.3 -4.8 -4.5 -2.0 -1.6 -0.8 -1.2 1.0 0 -1.8 -4.2 -3.9 – – –
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 – – –
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 13.295 16.5946 14.7211 14.4105 13.7248 14.7298 15.2336 17.3373 16.4744 14.0084 12.7878 16.1865 15.0051 20.0802 19.4052 19.5747 17.7278 19.1686
Actin 0.028 0 0.0202 0 0 0.0003 0.0452 0 0.0058 0.0525 0.0097 0.0049 0.0286 0 0.0117 0 0.0046 0.0015
Bud 0.0007 0 0.0004 0 0.0001 0.0001 0.0007 0.0002 0.0003 0.0005 0.0002 0 0.0017 0 0.0006 0 0.0001 0.0001
Bud Neck 0.0022 0 0.0082 0 0.0001 0.0003 0.0009 0.0001 0.0002 0.0015 0.0006 0.0002 0.0006 0 0.0004 0 0.0001 0.0001
Bud Periphery 0.0008 0 0.0004 0 0.0001 0.0001 0.0008 0.0002 0.0004 0.0011 0.0002 0.0001 0.0025 0 0.0009 0 0.0001 0.0001
Bud Site 0.0031 0 0.0026 0 0.0002 0.0002 0.0027 0.0019 0.0006 0.0006 0.0004 0.0001 0.005 0 0.0017 0 0.0001 0
Cell Periphery 0.0005 0 0.0003 0 0.0003 0.0001 0.0005 0.0001 0.0003 0.0003 0.0002 0 0.0005 0 0.0001 0 0.0001 0.0001
Cytoplasm 0.0112 0 0.0008 0 0 0.0001 0.0098 0.0006 0.0008 0.0002 0.0005 0 0.0021 0.0019 0.0015 0 0.0002 0.0001
Cytoplasmic Foci 0.0147 0 0.0048 0 0.0001 0.0078 0.0314 0 0.0043 0.0012 0.012 0.0018 0.0157 0.0001 0.0043 0 0.0002 0.0001
Eisosomes 0.0002 0 0.0002 0 0 0 0.0003 0 0.0001 0.0004 0.0001 0.0001 0.0004 0 0.0001 0 0 0
Endoplasmic Reticulum 0.0044 0 0.0006 0 0 0.0001 0.0025 0 0.0004 0.0006 0.0004 0 0.0041 0.0004 0.0013 0 0.0013 0.0003
Endosome 0.057 0 0.0023 0 0 0.0018 0.0198 0 0.0069 0.0017 0.0108 0.0002 0.0111 0.0016 0.0139 0 0.0032 0.0022
Golgi 0.0087 0 0.0027 0 0 0.0008 0.009 0 0.0015 0.0042 0.0052 0.0003 0.003 0 0.0103 0 0.0007 0.0006
Lipid Particles 0.0256 0 0.0078 0 0.0001 0.005 0.0195 0 0.0068 0.0031 0.0254 0.0011 0.006 0 0.0034 0 0.0003 0.0002
Mitochondria 0.0244 0.0001 0.0047 0.0311 0.0523 0.0017 0.0077 0.0001 0.0073 0.0232 0.0026 0.0008 0.0145 0.0002 0.0067 0 0.0036 0.0025
None 0.0046 0 0.0005 0 0 0.0001 0.0047 0 0.0006 0.0002 0.0009 0.0002 0.0044 0.0002 0.0007 0 0.0002 0.0001
Nuclear Periphery 0.0154 0.0006 0.0089 0.0001 0.0001 0.0036 0.008 0.0004 0.0012 0.0007 0.0033 0.0002 0.0451 0.0027 0.014 0 0.0527 0.0039
Nucleolus 0.1628 0.0657 0.085 0.0592 0.5803 0.3429 0.1461 0.0688 0.0747 0.051 0.5713 0.307 0.1608 0.0786 0.0665 0.0395 0.506 0.299
Nucleus 0.5829 0.9334 0.8402 0.9092 0.3649 0.6326 0.6604 0.9269 0.8833 0.8379 0.2943 0.6788 0.6586 0.9135 0.849 0.9604 0.4237 0.6882
Peroxisomes 0.0143 0 0.0055 0 0.0001 0.0008 0.0069 0 0.0013 0.0177 0.0422 0.0005 0.0025 0 0.0038 0 0.0001 0
Punctate Nuclear 0.0216 0.0001 0.001 0.0001 0.0005 0.0006 0.0188 0.0002 0.0012 0.0003 0.019 0.0033 0.0248 0.0001 0.0048 0 0.0011 0.0004
Vacuole 0.0139 0 0.0011 0 0.0004 0.0005 0.0019 0.0003 0.0014 0.0005 0.0004 0.0001 0.0024 0.0004 0.0027 0 0.0002 0.0002
Vacuole Periphery 0.0031 0 0.0018 0 0.0005 0.0005 0.0023 0.0001 0.0006 0.0007 0.0003 0.0001 0.0054 0.0003 0.0015 0 0.0013 0.0004

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 40.9309 34.013 32.1225 49.9281 42.6044 33.418 39.6012 49.236 43.8749 48.7322
Translational Efficiency 1.9364 2.7428 2.4088 1.5168 1.9551 2.4137 2.6404 1.7589 1.8058 1.9662

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
50 264 217 1203 1699 1456 406 939 1749 1720 623 2142

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 1691.42 1846.77 2305.03 1760.14 1560.27 1831.73 2062.99 2008.51 1564.02 1834.04 2147.30 1869.02
Standard Deviation 392.78 436.70 424.70 452.21 373.48 398.02 364.90 571.72 374.68 404.23 403.60 522.80
Intensity Change Log 2 — 0.126769 0.446551 0.057455 — 0.231411 0.402941 0.364330 — 0.177928 0.425790 0.212858

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000717 0.000735 0.000968 0.001284 0.000590 0.000689 0.001039 0.001063 0.000594 0.000696 0.001014 0.001187
Bud Neck 0.003458 0.006249 0.008081 0.019467 0.003467 0.005131 0.008596 0.016437 0.003466 0.005302 0.008416 0.018139
Bud Site 0.000894 0.001378 0.001033 0.007076 0.000650 0.000850 0.001183 0.003662 0.000657 0.000931 0.001131 0.005579
Cell Periphery 0.000038 0.000095 0.000021 0.000332 0.000052 0.000072 0.000027 0.000209 0.000052 0.000075 0.000025 0.000278
Cytoplasm 0.000034 0.000118 0.000020 0.003334 0.000593 0.000343 0.000052 0.002995 0.000577 0.000309 0.000041 0.003185
Cytoplasmic Foci 0.000792 0.000790 0.000633 0.005631 0.000384 0.000405 0.000743 0.004589 0.000396 0.000465 0.000705 0.005174
Eisosomes 0.000176 0.000167 0.000225 0.000086 0.000152 0.000210 0.000216 0.000070 0.000152 0.000204 0.000219 0.000079
Endoplasmic Reticulum 0.000335 0.000393 0.000196 0.000789 0.000261 0.000268 0.000332 0.000423 0.000263 0.000287 0.000285 0.000629
Endosome 0.000014 0.000118 0.000006 0.005490 0.000072 0.000040 0.000011 0.001920 0.000070 0.000052 0.000009 0.003925
Golgi 0.000054 0.000105 0.000089 0.002554 0.000055 0.000069 0.000119 0.003051 0.000055 0.000074 0.000108 0.002772
Lipid Particles 0.000005 0.000085 0.000007 0.001057 0.000019 0.000026 0.000011 0.000361 0.000019 0.000035 0.000009 0.000752
Mitochondria 0.001348 0.001671 0.002557 0.005817 0.001160 0.001567 0.002295 0.005058 0.001165 0.001583 0.002386 0.005484
Mitotic Spindle 0.000122 0.002926 0.000104 0.013235 0.000342 0.001771 0.000324 0.020997 0.000335 0.001948 0.000247 0.016638
None 0.000600 0.000764 0.000682 0.004046 0.001000 0.001124 0.000911 0.002486 0.000989 0.001069 0.000832 0.003362
Nuclear Periphery 0.000061 0.000067 0.000029 0.000915 0.000048 0.000058 0.000049 0.000901 0.000048 0.000060 0.000042 0.000909
Nuclear Periphery Foci 0.000008 0.000368 0.000003 0.001505 0.000052 0.000089 0.000008 0.000767 0.000051 0.000132 0.000006 0.001181
Nucleolus 0.215302 0.158224 0.123001 0.059274 0.132940 0.142338 0.151847 0.059682 0.135294 0.144777 0.141799 0.059453
Nucleus 0.754614* 0.810133* 0.832356* 0.857856* 0.851421* 0.837649* 0.800498* 0.868980* 0.848653* 0.833425* 0.811595* 0.862732*
Peroxisomes 0.020989 0.011333 0.029404 0.003918 0.006184 0.005824 0.031154 0.003495 0.006607 0.006670 0.030544 0.003733
Vacuole 0.000205 0.003311 0.000233 0.004001 0.000217 0.000623 0.000276 0.001622 0.000217 0.001036 0.000261 0.002958
Vacuole Periphery 0.000233 0.000971 0.000352 0.002335 0.000343 0.000853 0.000312 0.001232 0.000340 0.000871 0.000326 0.001851

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -0.14 -2.80 -4.13 -4.13 -2.41 -1.97 -11.38 -10.13 -7.21 -1.59 -2.25 -12.47 -10.02 -7.54 -2.63
Bud Neck -1.60 -3.44 -8.40 -7.56 -7.55 -5.48 -10.47 -13.19 -11.83 -7.84 -5.78 -13.23 -16.51 -14.46 -10.36
Bud Site -0.51 -0.28 -4.21 -4.17 -4.77 -0.41 -2.88 -5.19 -4.99 -4.26 -0.68 -2.64 -6.71 -6.46 -6.05
Cell Periphery -0.69 0.59 -4.64 -2.65 -6.93 -0.77 1.62 -4.05 -3.21 -4.99 -0.94 1.80 -6.68 -5.16 -8.49
Cytoplasm -1.22 0.54 -3.34 -3.27 -3.36 0.81 1.51 -2.00 -2.66 -3.06 0.88 1.54 -3.07 -3.98 -4.42
Cytoplasmic Foci 0.04 0.29 -3.00 -3.54 -4.14 0.39 -1.16 -3.12 -3.29 -2.83 0.16 -1.05 -5.06 -5.37 -4.89
Eisosomes 0.31 -0.83 1.74 3.18 9.79 -4.68 -6.40 9.56 12.28 15.00 -4.28 -7.31 9.51 12.33 17.60
Endoplasmic Reticulum -0.42 1.82 -3.73 -2.40 -5.40 -0.30 -3.05 -6.39 -6.16 -4.41 -0.73 -1.69 -6.41 -5.84 -5.95
Endosome -1.56 0.85 -4.17 -4.05 -4.18 0.72 1.45 -2.76 -2.83 -2.89 0.42 1.51 -4.85 -4.89 -4.97
Golgi -1.46 -2.78 -3.66 -3.54 -3.57 -0.62 -6.16 -2.09 -2.07 -2.01 -1.01 -5.85 -3.63 -3.60 -3.51
Lipid Particles -1.76 -1.18 -1.65 -1.47 -1.65 -0.27 0.82 -2.80 -2.77 -2.92 -0.82 0.94 -2.10 -2.05 -2.14
Mitochondria -0.53 -3.27 -4.55 -4.05 -3.13 -3.63 -9.25 -3.84 -3.43 -2.58 -3.50 -10.50 -6.38 -5.67 -4.16
Mitotic Spindle -1.43 0.14 -5.54 -3.06 -5.56 -1.53 -0.22 -5.89 -5.40 -5.88 -1.91 0.22 -7.90 -6.68 -7.96
None -1.39 -1.15 -5.59 -5.26 -5.44 -0.25 0.39 -4.37 -3.81 -6.70 -0.15 0.67 -5.33 -5.23 -7.09
Nuclear Periphery 0.03 0.64 -4.52 -4.75 -5.08 -0.63 -0.65 -3.66 -3.62 -3.64 -0.75 0.29 -5.91 -5.82 -5.93
Nuclear Periphery Foci -1.60 1.51 -2.29 -1.43 -2.30 -0.42 1.88 -3.73 -3.46 -4.11 -1.10 1.95 -3.07 -2.79 -3.28
Nucleolus 1.77 2.97 5.22 8.29 6.74 -1.45 -2.84 11.94 12.82 10.24 -1.57 -1.63 14.55 16.18 12.36
Nucleus -1.60 -2.25 -3.33 -3.47 -2.50 1.93 5.47 -0.57 -2.02 -5.24 2.29 5.09 -1.43 -3.57 -6.00
Peroxisomes 2.31 -1.68 4.44 5.35 6.90 0.69 -8.88 5.30 4.78 9.81 -0.14 -10.60 6.61 7.07 12.01
Vacuole -1.62 -0.51 -3.75 -0.38 -3.72 -1.89 -1.69 -4.58 -3.09 -4.37 -2.39 -1.49 -4.93 -3.09 -4.81
Vacuole Periphery -1.59 -1.67 -2.86 -1.13 -2.64 -3.62 0.46 -1.65 -0.46 -1.70 -3.82 0.11 -3.14 -1.51 -3.16
Standard name
Human Ortholog
Description RNA polymerase II third largest subunit B44; part of central core; similar to prokaryotic alpha subunit
Localization
Cell Percentages nucleus (84%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-2

Rpb3

Rpb3


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Rpb3-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available