Standard name
Human Ortholog
Description Component of the CCR4-NOT core complex, involved in mRNA decapping; involved in transcription initiation and elongation and in mRNA degradation; conserved lysine in human homolog of Not3p and Not5p is mutated in cancers
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0.05 0 0.05 0.05 0.05 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.96 0.96 0.94 0.88 0.89 0.85 0.78 – 0.6 0.49 0.53 0.59 0.97 0.96 0.97 0.89 0.9 0.85 0.95 0.93 0.9 0.69 0.74 0.8
Endoplasmic Reticulum 0 0 0.05 0 0.09 0 0 – 0 0 0 0.06 0 0 0 0 0 0.05 0 0 0 0.21 0.2 0.13
Endosome 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.19 0.17 – 0.47 0.51 0.52 0.34 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0.05 – 0.06 0.07 0.05 0.1 0 0 0 0.13 0.07 0.12 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 1 1 2 4 – 2 2 0 5 1 0 0 0 0 1 0 0 1 1 0 0
Bud 0 1 1 0 0 0 3 – 2 1 4 0 3 2 1 0 0 1 0 1 1 0 1 0
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 12 7 10 3 6 2 7 – 8 5 5 4 4 5 6 6 4 4 0 0 0 0 0 0
Cytoplasm 241 323 175 58 108 124 165 – 107 86 70 96 328 351 349 152 118 94 251 316 190 198 259 270
Endoplasmic Reticulum 2 12 9 1 11 4 6 – 2 4 5 10 7 14 7 5 4 6 4 7 6 60 70 44
Endosome 0 0 0 0 0 0 4 – 0 0 0 1 0 0 1 0 0 1 1 1 1 1 0 0
Golgi 0 1 0 1 0 0 1 – 0 0 0 0 0 0 0 0 0 0 0 2 1 1 0 1
Mitochondria 2 0 0 1 1 28 35 – 84 89 69 56 4 1 7 3 4 3 0 1 4 4 4 3
Nucleus 4 0 0 0 1 3 1 – 2 3 4 5 1 0 1 2 2 1 0 1 0 0 0 2
Nuclear Periphery 0 0 0 0 0 0 0 – 1 2 0 1 1 0 0 0 1 0 1 0 0 2 0 1
Nucleolus 0 0 0 0 0 0 0 – 2 1 1 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1
SpindlePole 0 0 0 0 0 0 0 – 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 2 1 2 4 3 10 – 10 12 6 16 0 5 0 23 9 13 3 7 3 8 7 8
Unique Cell Count 252 335 186 66 121 146 212 178 174 132 163 339 367 359 171 131 110 264 339 212 286 352 339
Labelled Cell Count 261 346 196 67 132 166 236 222 205 164 194 349 378 372 191 142 124 264 339 212 286 352 339


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 13.9 13.2 11.0 9.8 9.5 7.6 8.2 – 6.9 6.0 6.2 6.6 10.6 11.1 10.8 15.3 15.7 13.2 10.7 10.9 11.5
Std Deviation (1e-4) 2.3 2.5 1.4 1.6 1.4 1.3 1.4 – 1.4 1.1 1.2 1.3 1.6 1.6 1.6 3.8 3.9 4.1 1.5 1.8 1.7
Intensity Change (Log2) – – – -0.16 -0.2 -0.52 -0.42 – -0.67 -0.88 -0.83 -0.73 -0.05 0.02 -0.02 0.48 0.52 0.26 -0.03 -0.01 0.07


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 -0.2 -1.9 -1.0 – -0.4 -1.2 -0.7 -1.4 0 -2.7 -2.4 -0.9 -1.0 -0.7
Cytoplasm -1.6 -1.5 -2.8 -4.6 – -7.8 -9.5 -8.6 -7.9 1.5 0.8 1.8 -1.8 -1.3 -2.5
Endoplasmic Reticulum 0 1.5 -1.0 -1.0 – -2.1 -1.3 -0.5 0.5 -1.8 -0.6 -1.9 -0.9 -0.8 0.2
Endosome 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 6.2 5.8 – 10.7 11.2 11.1 8.7 0 0 0 0 0 0
Nucleus 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 2.5 – 2.8 3.2 0 4.0 0 0 0 4.9 0 4.4

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 8.2665 10.7277 9.6079 9.4311 9.0232 9.801 8.0062 10.6956 9.345 9.4641 8.5451 9.9153 8.1731 10.1436 9.3261 9.1713 8.6734 9.5077
Actin 0.0137 0.0006 0.0122 0.001 0.0005 0.0011 0.0208 0.0019 0.005 0.0373 0.003 0.002 0.0085 0.0003 0.0009 0.0004 0.001 0.0015
Bud 0.0009 0.0004 0.0007 0.0026 0.0007 0.0005 0.001 0.0006 0.0018 0.0009 0.0009 0.0005 0.0004 0.0003 0.0004 0.0005 0.0002 0.0002
Bud Neck 0.0008 0.0003 0.0009 0.0011 0.0011 0.0012 0.0022 0.0006 0.0026 0.001 0.0007 0.0012 0.0029 0.0002 0.0005 0.0013 0.0007 0.0009
Bud Periphery 0.0012 0.0002 0.0006 0.0023 0.0002 0.0008 0.0013 0.0005 0.0015 0.0007 0.0008 0.0004 0.0004 0.0002 0.0003 0.0003 0.0002 0.0001
Bud Site 0.0016 0.0013 0.0014 0.0021 0.0005 0.0002 0.0021 0.0034 0.0028 0.0025 0.0003 0.0001 0.0012 0.0007 0.0007 0.0001 0.0001 0.0001
Cell Periphery 0.0011 0.0003 0.0009 0.0008 0.0001 0.0006 0.0007 0.0015 0.0004 0.0003 0.0001 0.0001 0.0005 0.0017 0.0004 0.0002 0.0001 0.0001
Cytoplasm 0.6153 0.9111 0.7805 0.7682 0.8418 0.8112 0.6447 0.9313 0.8148 0.7122 0.7862 0.8031 0.7576 0.9188 0.8472 0.8118 0.8608 0.8668
Cytoplasmic Foci 0.0404 0.0098 0.021 0.0267 0.0253 0.0211 0.025 0.0092 0.0216 0.0244 0.0218 0.0163 0.0148 0.0111 0.0167 0.015 0.0134 0.0131
Eisosomes 0.0005 0 0.0006 0 0 0 0.0001 0.0001 0 0.0001 0 0 0.0001 0 0 0 0 0
Endoplasmic Reticulum 0.0951 0.0333 0.0351 0.0437 0.0129 0.0527 0.081 0.0143 0.0279 0.0138 0.0134 0.0667 0.098 0.0265 0.0414 0.0301 0.0305 0.0426
Endosome 0.1084 0.0269 0.0652 0.1012 0.0793 0.0691 0.0998 0.0183 0.067 0.1123 0.068 0.0685 0.0609 0.0255 0.0557 0.0919 0.0545 0.0454
Golgi 0.0164 0.0028 0.0104 0.0064 0.0057 0.0086 0.0165 0.0028 0.0094 0.0113 0.0259 0.0075 0.0063 0.0023 0.0058 0.0029 0.0024 0.0039
Lipid Particles 0.0116 0.0001 0.0074 0.0003 0.0002 0.0021 0.006 0.0003 0.0011 0.0021 0.0166 0.0006 0.0023 0.0003 0.0007 0.0001 0.0009 0.0009
Mitochondria 0.0217 0.0013 0.014 0.0084 0.0078 0.0119 0.0185 0.0032 0.0053 0.0021 0.0098 0.0071 0.0041 0.0013 0.0051 0.0022 0.0018 0.0104
None 0.0126 0.0013 0.0111 0.004 0.0009 0.0015 0.0037 0.0006 0.0009 0.0009 0.001 0.0009 0.0025 0.0007 0.001 0.0007 0.0015 0.001
Nuclear Periphery 0.013 0.0018 0.0099 0.0026 0.0021 0.0016 0.0216 0.0009 0.0043 0.0033 0.0233 0.005 0.0099 0.0013 0.0027 0.0021 0.0163 0.0033
Nucleolus 0.0004 0 0.0007 0.0001 0 0 0.0009 0 0.0001 0.0001 0.0009 0 0.0002 0 0.0001 0 0.0001 0
Nucleus 0.0044 0.001 0.0027 0.0021 0.0015 0.001 0.011 0.0011 0.0021 0.0022 0.0027 0.0101 0.0032 0.0009 0.0014 0.0013 0.0041 0.0014
Peroxisomes 0.0076 0.0001 0.0022 0.0002 0.0003 0.0052 0.0028 0.0001 0.0007 0.0034 0.0025 0.0002 0.0062 0.0001 0.0005 0.0001 0.0001 0.0006
Punctate Nuclear 0.0058 0.0001 0.0008 0.0002 0.0001 0.0006 0.0027 0.0001 0.0007 0.0026 0.0136 0.0002 0.0043 0.0001 0.0002 0.0001 0.001 0.0003
Vacuole 0.0206 0.0057 0.0182 0.0213 0.0148 0.0062 0.0301 0.008 0.0257 0.0551 0.0053 0.0062 0.0133 0.0063 0.0155 0.0312 0.0074 0.0052
Vacuole Periphery 0.007 0.0015 0.0036 0.0045 0.0042 0.0029 0.0074 0.0013 0.0044 0.0112 0.0031 0.0033 0.0028 0.0013 0.0027 0.0079 0.0028 0.0021

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 28.3431 20.8196 30.898 37.3864 31.9053 21.0809 28.0013 30.158 29.5954 33.8051
Translational Efficiency 2.3213 2.4197 1.6716 1.2938 1.8418 2.7083 1.3477 1.3643 1.2895 1.3211

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Component of the CCR4-NOT core complex, involved in mRNA decapping; involved in transcription initiation and elongation and in mRNA degradation; conserved lysine in human homolog of Not3p and Not5p is mutated in cancers
Localization
Cell Percentages cytoplasm (98%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-5

Not3

Not3


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Not3-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available