Standard name
Human Ortholog
Description Lysine methyltransferase; involved in the dimethylation of eEF1A (Tef1p/Tef2p) at lysine 316; sequence similarity to S-adenosylmethionine-dependent methyltransferases of the seven beta-strand family; role in vesicular transport
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0.09 0 0 0 0 0 0 0 0
Cytoplasm 0.87 0.89 0.96 0.96 0.97 0.9 0.88 0.88 0.72 0.74 0.59 0.69 – 0.94 0.95 0.98 1.0 0.97 0.81 0.73 0.86 0.85 0.87 0.82
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 – 0.06 0 0.06 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.11 0.13 0 0 0 0.09 0.11 0.08 0.32 0.3 0.46 0.37 – 0 0 0 0 0 0.05 0.06 0.05 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0.05 0 – 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0.08 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 1 0 1 0 0 – 0 0 0 0 0 0 1 3 0 0 0
Bud 1 0 0 2 1 3 4 0 14 4 11 7 – 0 0 0 0 0 0 0 1 0 0 1
Bud Neck 0 0 8 11 6 6 17 17 5 11 6 11 – 0 0 0 0 0 2 0 4 0 0 0
Bud Site 0 0 0 0 0 0 0 0 6 2 4 4 – 0 0 0 0 0 – – – – – –
Cell Periphery 0 1 5 2 3 1 2 3 0 2 0 2 – 0 0 8 4 4 0 0 0 0 0 0
Cytoplasm 48 90 298 342 465 401 497 441 329 286 177 317 – 17 86 86 164 129 60 88 276 18 39 55
Endoplasmic Reticulum 0 0 2 0 0 0 0 0 0 1 1 0 – 1 0 5 5 5 0 0 1 0 0 0
Endosome 0 2 1 3 1 2 2 3 3 0 2 4 – 0 2 0 0 1 1 2 2 0 0 0
Golgi 0 0 0 0 1 0 0 0 0 0 0 0 – 0 0 0 0 1 0 1 2 0 0 0
Mitochondria 6 13 1 7 6 42 63 40 146 118 139 172 – 0 1 2 3 0 3 7 15 0 0 2
Nucleus 0 1 1 2 1 1 4 3 9 6 10 11 – 0 1 1 0 3 0 2 0 0 0 0
Nuclear Periphery 0 1 0 0 2 0 0 1 0 0 0 1 – 0 1 0 0 0 0 0 0 0 0 0
Nucleolus 1 0 0 0 0 0 1 2 2 1 0 2 – 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 1 0 0 0 0 1 0 0 1 0 3 – 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 1 2 1 8 8 16 14 14 17 14 20 – 0 0 0 0 0 1 1 2 0 0 1
Vac/Vac Membrane 1 1 5 6 9 4 6 5 8 4 1 4 – 0 2 0 0 1 2 9 3 0 1 2
Unique Cell Count 55 101 311 358 481 445 566 499 459 388 302 459 18 91 88 164 133 75 122 321 22 45 67
Labelled Cell Count 57 111 323 376 503 468 613 530 536 454 365 558 18 93 102 176 144 75 122 321 22 45 67


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.8 4.1 5.4 5.3 5.4 4.2 4.1 4.5 3.5 3.6 3.3 3.5 – 4.8 5.3 7.3 8.5 9.4 – – 6.9
Std Deviation (1e-4) 0.4 0.9 1.3 1.9 1.3 1.3 1.1 1.7 1.2 1.0 0.8 0.8 – 1.2 1.8 1.0 1.3 1.7 – – 3.4
Intensity Change (Log2) – – – -0.03 0.01 -0.37 -0.39 -0.25 -0.64 -0.6 -0.7 -0.6 – -0.18 -0.04 0.44 0.65 0.79 – – 0.36


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration -0.5691 -0.7694 -0.6361 -0.8097 -0.8158 -0.8505 1.5154 1.4844 1.3345 0.9508 1.2232 1.4797 -0.9884 -0.4609 -0.6828 -1.4242 -1.2089 -1.0249
Actin 0.0468 0.0045 0.0162 0.0095 0.0364 0.0068 0.0514 0.0004 0.0138 0.0004 0.0037 0.0062 0.007 0.0001 0.0077 0.0001 0.0001 0.0093
Bud 0.0023 0.0013 0.0009 0.0004 0.0007 0.0026 0.0007 0.0005 0.0022 0 0.0038 0.0009 0.0012 0.0001 0.0011 0 0 0.0012
Bud Neck 0.002 0.0003 0.0026 0.0002 0.0013 0.0009 0.0069 0.0001 0.0005 0.0004 0.0007 0.0003 0.0007 0.0001 0.0008 0 0.0001 0.0011
Bud Periphery 0.0029 0.0021 0.0018 0.001 0.0009 0.0021 0.0011 0.0009 0.0029 0.0001 0.0128 0.0035 0.0031 0.0001 0.0018 0 0 0.0011
Bud Site 0.0161 0.0059 0.0079 0.0008 0.0032 0.0006 0.0026 0.0041 0.0037 0 0.0115 0.0002 0.0111 0.001 0.0037 0 0 0.0007
Cell Periphery 0.0007 0.0004 0.0002 0.0004 0.0002 0.0001 0.0006 0.0004 0.0002 0.0001 0.0005 0.0001 0.0003 0.0002 0.0002 0 0 0.0001
Cytoplasm 0.1819 0.3681 0.267 0.195 0.3775 0.325 0.1231 0.1877 0.1351 0.1746 0.1104 0.221 0.2152 0.2472 0.2224 0.3064 0.3722 0.4274
Cytoplasmic Foci 0.0281 0.0091 0.0189 0.0103 0.0162 0.0144 0.0288 0.0024 0.0132 0.003 0.0087 0.0086 0.0328 0.0067 0.0094 0.0031 0.0031 0.0116
Eisosomes 0.0009 0.0003 0.0002 0.0005 0.0003 0.0001 0.0011 0.0001 0.0003 0.0002 0.0001 0.0001 0.0004 0.0001 0.0001 0 0.0002 0.0001
Endoplasmic Reticulum 0.0062 0.0042 0.0037 0.0136 0.0038 0.0018 0.0078 0.0005 0.0016 0.0007 0.0005 0.001 0.0037 0.0018 0.0024 0.0007 0.0004 0.0018
Endosome 0.0251 0.0065 0.0103 0.0105 0.0311 0.0065 0.0206 0.0003 0.0053 0.0007 0.0158 0.0068 0.0139 0.0029 0.0042 0.0004 0.0005 0.0032
Golgi 0.0094 0.0007 0.0027 0.0029 0.0069 0.0023 0.0112 0.0001 0.0023 0.0001 0.0061 0.0017 0.0029 0.0002 0.001 0 0 0.0005
Lipid Particles 0.0185 0.0007 0.0092 0.0028 0.0097 0.0036 0.0228 0.0001 0.0051 0.0001 0.0022 0.0055 0.0064 0.001 0.0039 0.0001 0 0.0009
Mitochondria 0.0181 0.0011 0.0077 0.0125 0.0042 0.0029 0.0087 0.0001 0.0018 0.0002 0.0029 0.0059 0.0017 0.0002 0.0008 0.0001 0.0001 0.0004
None 0.6014 0.5749 0.6257 0.729 0.4871 0.6245 0.6699 0.8013 0.8005 0.8178 0.8113 0.7312 0.678 0.7347 0.7254 0.6879 0.6223 0.5364
Nuclear Periphery 0.0079 0.0015 0.0051 0.0043 0.0075 0.0013 0.0061 0.0001 0.0012 0.0002 0.0002 0.0004 0.0049 0.0006 0.0067 0.0002 0.0001 0.0007
Nucleolus 0.0019 0.0002 0.0003 0.0001 0.0006 0.0001 0.0014 0.0001 0.0003 0.0001 0.0003 0.0001 0.0007 0.0001 0.0004 0 0 0.0002
Nucleus 0.0034 0.0017 0.0018 0.001 0.0027 0.0014 0.0038 0.0003 0.001 0.0006 0.0004 0.0003 0.0017 0.0008 0.0029 0.0003 0.0002 0.0008
Peroxisomes 0.0141 0.0126 0.0126 0.0006 0.0035 0.0006 0.0178 0 0.004 0 0.0042 0.0048 0.0071 0.0001 0.0013 0 0 0.0002
Punctate Nuclear 0.0065 0.001 0.0021 0.0006 0.0028 0.0011 0.0089 0.0002 0.0042 0.0002 0.0003 0.0005 0.0052 0.0004 0.0021 0.0002 0.0002 0.0009
Vacuole 0.0041 0.0021 0.002 0.0024 0.0023 0.0008 0.0038 0.0004 0.0008 0.0005 0.0032 0.0007 0.0017 0.0015 0.0014 0.0003 0.0002 0.001
Vacuole Periphery 0.0016 0.0007 0.0012 0.0014 0.001 0.0003 0.0011 0 0.0002 0.0001 0.0006 0.0003 0.0005 0.0001 0.0005 0 0 0.0002

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 54.1723 54.7987 39.7173 30.8664 47.1326 37.0447 28.6698 20.0025 29.2805 31.1502
Translational Efficiency 1.0815 0.9993 1.166 1.4157 0.9272 1.1023 1.0568 1.5273 0.9103 1.0773

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1130 196 2428 1674 1407 1802 170 1299 2537 1998 2598 2973

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 638.43 700.35 866.29 838.36 669.21 727.83 937.12 797.88 655.50 725.13 870.92 820.67
Standard Deviation 73.06 101.21 100.01 113.93 79.95 86.87 105.10 115.29 78.46 88.76 101.87 116.28
Intensity Change Log 2 — 0.133548 0.440322 0.393041 — 0.121143 0.485775 0.253713 — 0.127212 0.463762 0.323419

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000126 0.000508 0.000122 0.000632 0.000060 0.000165 0.000450 0.000194 0.000089 0.000199 0.000143 0.000440
Bud Neck 0.072451 0.078205 0.004585 0.007369 0.029668 0.062581 0.010480 0.003035 0.048724 0.064114 0.004970 0.005475
Bud Site 0.015539 0.065111 0.009157 0.035077 0.008845 0.024294 0.006164 0.015090 0.011827 0.028298 0.008961 0.026344
Cell Periphery 0.000240 0.000211 0.000079 0.000118 0.000124 0.000149 0.000335 0.000128 0.000176 0.000155 0.000096 0.000123
Cytoplasm 0.434313* 0.299295* 0.566690* 0.624057* 0.563704* 0.465991* 0.336599* 0.697077* 0.506072* 0.449639* 0.551634* 0.655962*
Cytoplasmic Foci 0.215956* 0.187426* 0.007394 0.021202 0.143781 0.158726 0.060029 0.038469 0.175929 0.161542 0.010838 0.028746
Eisosomes 0.000184 0.000133 0.000016 0.000030 0.000072 0.000101 0.000155 0.000032 0.000122 0.000104 0.000025 0.000031
Endoplasmic Reticulum 0.000714 0.000983 0.001410 0.000985 0.000514 0.000411 0.004955 0.000709 0.000603 0.000468 0.001642 0.000864
Endosome 0.028818 0.067960 0.001263 0.004618 0.012440 0.025661 0.011101 0.003084 0.019735 0.029810 0.001907 0.003948
Golgi 0.002509 0.003162 0.000072 0.002030 0.001003 0.003082 0.000156 0.000570 0.001674 0.003090 0.000077 0.001392
Lipid Particles 0.008877 0.004793 0.000541 0.000636 0.003384 0.003761 0.007165 0.000694 0.005830 0.003862 0.000975 0.000661
Mitochondria 0.009814 0.008485 0.000616 0.002957 0.003937 0.012182 0.000507 0.000259 0.006555 0.011819 0.000609 0.001778
Mitotic Spindle 0.000290 0.003315 0.007329 0.037303 0.000206 0.003407 0.003525 0.005504 0.000243 0.003398 0.007080 0.023409
None 0.004346 0.001796 0.004700 0.004880 0.005464 0.004556 0.002261 0.004382 0.004966 0.004285 0.004541 0.004662
Nuclear Periphery 0.000564 0.000438 0.001671 0.001260 0.000286 0.000303 0.001658 0.000378 0.000410 0.000316 0.001670 0.000875
Nuclear Periphery Foci 0.000346 0.000723 0.000638 0.001507 0.000234 0.000168 0.006392 0.001202 0.000284 0.000223 0.001015 0.001374
Nucleolus 0.003376 0.002810 0.000337 0.000275 0.001709 0.002206 0.001113 0.000550 0.002451 0.002265 0.000388 0.000395
Nucleus 0.102335 0.079682 0.351971* 0.107963 0.161842* 0.110571 0.355453* 0.101765 0.135337* 0.107541 0.352198* 0.105255
Peroxisomes 0.008089 0.028805 0.000112 0.001238 0.002548 0.010259 0.000894 0.000460 0.005016 0.012078 0.000163 0.000898
Vacuole 0.087764 0.160271 0.041078 0.142342 0.058312 0.108534 0.190249 0.125489 0.071430 0.113609 0.050839 0.134978
Vacuole Periphery 0.003349 0.005889 0.000217 0.003523 0.001868 0.002891 0.000359 0.000927 0.002528 0.003185 0.000226 0.002389

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -3.31 -1.56 -3.00 1.06 -2.67 -6.87 -2.44 -7.94 -2.15 1.88 -5.98 -4.67 -4.66 -2.07 -2.17
Bud Neck -0.26 14.78 14.66 7.01 -0.65 -8.31 4.32 11.16 19.07 1.09 -3.80 17.55 18.01 20.02 1.19
Bud Site -5.10 5.48 -0.35 4.96 -4.32 -7.50 0.99 -1.99 4.81 -2.26 -7.92 3.96 -1.93 5.83 -4.75
Cell Periphery 0.97 11.28 10.88 3.78 0.04 -1.53 -6.69 0.82 1.75 6.37 1.36 9.81 8.06 3.89 0.21
Cytoplasm 6.59 -6.18 -7.42 -10.58 -2.17 10.11 14.08 -5.64 -15.62 -16.90 6.81 3.27 -7.26 -13.45 -10.65
Cytoplasmic Foci 1.84 38.18 35.70 13.58 -12.10 -2.72 14.08 24.56 27.69 1.90 2.62 48.36 42.70 33.75 -15.86
Eisosomes 3.84 24.06 21.95 9.10 -8.68 -3.66 -7.11 11.89 8.40 9.67 2.29 23.87 23.89 9.90 -2.24
Endoplasmic Reticulum -1.35 -11.21 -3.96 -1.11 8.07 1.74 -9.18 -5.62 -7.82 8.57 2.21 -16.70 -6.68 -9.63 12.45
Endosome -4.54 15.90 14.98 7.89 -2.88 -7.24 1.23 9.70 15.05 6.41 -5.25 18.48 17.54 16.81 -2.20
Golgi -0.98 6.89 3.51 3.63 -1.76 -3.99 9.04 2.91 4.94 -1.18 -2.84 9.62 3.95 4.88 -2.23
Lipid Particles 3.26 8.30 8.15 5.11 -3.10 -0.72 -4.24 8.96 6.43 7.74 3.10 9.96 10.65 7.20 3.81
Mitochondria 0.53 6.85 6.18 2.43 -1.59 -5.41 5.42 5.97 8.67 1.13 -3.55 8.56 7.98 8.51 -0.78
Mitotic Spindle -2.19 -7.38 -7.94 -5.40 -4.80 -3.73 -1.76 -5.35 -2.26 -2.22 -4.07 -7.58 -9.28 -6.44 -4.13
None 2.39 -0.27 0.32 -3.05 1.39 0.94 5.27 2.52 1.02 -3.19 0.83 0.99 1.92 0.48 1.82
Nuclear Periphery 0.63 -12.23 -4.25 -5.02 2.97 -0.83 -14.99 -7.27 -6.33 11.57 1.28 -22.43 -5.62 -6.73 7.96
Nuclear Periphery Foci -2.66 -6.64 -4.48 -1.71 -1.93 1.45 -6.94 -10.72 -11.52 5.70 1.12 -10.78 -8.29 -8.73 -0.36
Nucleolus 0.44 4.15 4.20 5.01 0.71 -1.49 2.80 2.66 3.45 0.62 0.35 5.96 5.31 5.80 -0.55
Nucleus 2.21 -43.75 -8.93 -7.19 36.13 8.64 -7.81 6.38 -2.11 9.71 5.89 -41.73 -0.97 -7.13 42.14
Peroxisomes -3.09 9.14 8.55 4.27 -2.68 -8.22 2.34 5.78 11.08 1.33 -6.33 11.08 10.13 11.21 -2.90
Vacuole -5.46 3.78 -22.32 -5.96 -26.80 -11.89 -11.45 -19.86 -10.96 4.75 -10.67 -3.30 -30.58 -19.24 -26.46
Vacuole Periphery -1.81 10.31 3.83 3.36 -2.21 -3.04 9.09 3.69 5.50 -0.97 -1.86 13.97 4.69 5.39 -2.59
Standard name
Human Ortholog
Description Lysine methyltransferase; involved in the dimethylation of eEF1A (Tef1p/Tef2p) at lysine 316; sequence similarity to S-adenosylmethionine-dependent methyltransferases of the seven beta-strand family; role in vesicular transport
Localization
Cell Percentages cytoplasm (52%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Efm4

Efm4


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Efm4-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available