Standard name
Human Ortholog
Description Component of RNA polymerase transcription factor TFIIH holoenzyme; acts as dsDNA-dependent translocase in context of TFIIH, unwinds DNA strands during initiation and promotes transcription start site (TSS) scanning; has DNA-dependent ATPase/helicase activity; interacts functionally with TFIIB, has roles in TSS selection and gene looping to juxtapose initiation and termination regions; involved in DNA repair; relocalizes to cytosol under hypoxia; homolog of human ERCC3
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 – 0 – 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 – 0 – 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 – 0 – 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 – 0 – 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 – 0 – 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.06 0 0 0 0.06 0 0 0 0 – 0 – 0 0.06 0.1 0.1 0.09 0.08 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 – 0 – 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 – 0 – 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 – 0 – 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.1 0.07 0 0.11 0 0.12 0.11 0.08 0.21 – 0.35 – 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0.85 0.92 0.95 0.91 0.92 0.9 0.93 0.9 0.86 – 0.8 – 0.95 0.91 0.87 0.85 0.81 0.84 0.79 0.91 0.9 0.84 0.79 0.7
Nuclear Periphery 0 0 0 0 0 0 0 0 0 – 0 – 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.18 0.15 0 0.06 0 0 0 0 0.06 – 0.06 – 0.06 0.06 0.06 0 0 0 0.08 0 0 0.05 0.1 0.12
Peroxisomes 0 0 0 0 0 0 0 0 0 – 0 – 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 – 0 – 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 – 0 – 0 0 0 0 0 0.06 0 0 0 0.05 0.07 0.09
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 – 0 – 0 0 0 0 0 0 0 0 0 0 0 3
Bud 0 0 0 1 0 0 2 1 1 – 0 – 0 1 1 0 0 0 0 1 1 1 3 8
Bud Neck 0 0 0 0 0 0 1 0 0 – 0 – 0 1 0 0 0 0 1 0 0 0 3 2
Bud Site 0 0 0 0 0 0 3 0 2 – 8 – 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 – 0 – 0 0 0 1 0 0 0 0 0 0 0 0
Cytoplasm 7 2 8 7 18 13 16 17 15 – 13 – 11 38 57 33 19 17 0 0 1 2 0 0
Endoplasmic Reticulum 0 0 0 0 2 0 1 0 0 – 0 – 0 0 0 3 6 7 0 0 0 1 0 1
Endosome 0 0 1 0 1 2 2 4 2 – 0 – 1 0 2 0 4 3 2 0 1 1 2 6
Golgi 0 0 0 0 0 0 1 0 0 – 0 – 0 0 0 1 1 0 1 0 0 2 0 3
Mitochondria 11 12 2 21 4 42 63 50 95 – 132 – 10 1 1 3 0 3 1 0 3 1 0 4
Nucleus 93 165 286 170 287 311 545 547 384 – 297 – 396 540 518 279 179 181 85 177 288 196 412 418
Nuclear Periphery 2 0 0 0 2 1 1 9 3 – 3 – 1 3 0 0 1 2 0 0 0 0 0 0
Nucleolus 20 27 1 12 3 8 17 27 25 – 22 – 26 36 37 11 8 3 8 7 8 12 53 69
Peroxisomes 0 0 0 0 0 0 0 1 0 – 0 – 0 3 3 0 0 0 0 0 0 0 0 1
SpindlePole 0 0 1 2 2 2 3 14 6 – 8 – 2 1 4 0 5 2 0 0 0 0 3 10
Vac/Vac Membrane 0 3 5 2 2 1 2 5 5 – 8 – 0 1 5 12 9 12 1 6 11 11 37 56
Unique Cell Count 109 179 302 186 312 345 587 606 448 373 419 595 593 329 221 215 108 196 322 235 522 596
Labelled Cell Count 133 209 304 215 321 380 657 675 538 491 447 625 628 343 232 230 108 196 322 235 522 596


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 6.4 7.1 7.1 6.1 7.7 6.1 6.0 6.2 5.3 – 4.7 – 7.9 8.1 7.5 10.4 10.2 10.3 6.8 6.5 7.2
Std Deviation (1e-4) 1.2 1.3 1.2 1.7 1.7 1.5 1.5 1.7 1.4 – 1.2 – 1.6 1.8 1.9 2.3 2.5 2.5 1.5 1.4 1.6
Intensity Change (Log2) – – – -0.23 0.11 -0.24 -0.25 -0.2 -0.44 – -0.61 – 0.15 0.18 0.06 0.54 0.51 0.53 -0.08 -0.13 0.01

WT3RAP60RAP140RAP220RAP300RAP380RAP460RAP6200510WT3HU80HU120HU1600510WT3rpd3Δ_1rpd3Δ_2rpd3Δ_30510WT1AF100AF140AF1800510
rapamycinhydroxy-urearpd3 knockoutalpha-factorMultiple Condition Time-Course Intensity PlotsGFP Intensity (1e-4)GFP Intensity (1e-4)GFP Intensity (1e-4)GFP Intensity (1e-4)

Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 – 0 – 0 0 0 0 0 0
Bud 0 0 0 0 0 0 – 0 – 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 – 0 – 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 – 0 – 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 – 0 – 0 0 0 0 0 0
Cytoplasm 0.7 1.9 0.8 0.1 0.1 0.5 – 0.6 – 0 2.4 3.8 3.8 3.0 2.7
Endoplasmic Reticulum 0 0 0 0 0 0 – 0 – 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 – 0 – 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 – 0 – 0 0 0 0 0 0
Mitochondria 5.4 0 5.8 5.5 4.6 8.2 – 0 – 1.8 0 0 0 0 0
Nucleus -1.4 -1.3 -2.2 -1.1 -2.3 -3.9 – -5.7 – -0.1 -2.1 -3.4 -4.1 -4.9 -4.0
Nuclear Periphery 0 0 0 0 0 0 – 0 – 0 0 0 0 0 0
Nucleolus 0 0 0 2.6 3.4 3.9 – 0 – 4.1 4.1 4.1 2.8 0 0
Peroxisomes 0 0 0 0 0 0 – 0 – 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 – 0 – 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 – 0.5 – 0 0 0 1.5 1.7 2.5

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 4.5666 5.2522 4.629 4.5921 4.4447 4.6634 2.2545 4.2324 3.187 2.7655 2.1087 2.6699 3.938 5.111 4.4262 4.3994 3.6427 4.0183
Actin 0.0308 0 0.0036 0 0.0329 0.0107 0.0118 0 0.0017 0 0.0002 0.0016 0.013 0 0.0077 0.0071 0.0046 0.0001
Bud 0.0001 0 0.0001 0 0.0002 0.0001 0.0006 0 0.0001 0 0.0002 0.0001 0.0002 0 0 0.0017 0.0012 0
Bud Neck 0.0005 0.0001 0.0003 0 0.0023 0.0009 0.0007 0 0.0001 0.0001 0.0005 0.0006 0.0003 0 0.0003 0.0009 0.0017 0.0007
Bud Periphery 0.0002 0 0.0004 0 0.0004 0.0001 0.0013 0 0.0001 0 0.0006 0.0003 0.0003 0 0.0001 0.0025 0.0022 0
Bud Site 0.0003 0 0.0008 0 0.002 0.0001 0.0035 0 0.0002 0 0.001 0.0002 0.0008 0 0.0001 0.0022 0.0012 0.0001
Cell Periphery 0 0 0.0004 0 0.0002 0.0001 0.0006 0 0 0 0.0006 0.0001 0.0002 0 0.0001 0.0003 0.0002 0
Cytoplasm 0.0006 0 0.0007 0 0.0002 0 0.0014 0 0.0002 0 0.0001 0.0001 0.0009 0 0.0001 0.0032 0.0043 0.0005
Cytoplasmic Foci 0.0032 0 0.0025 0 0.0041 0.0002 0.024 0 0.0053 0 0.0127 0.0007 0.004 0 0.0012 0.0031 0.0048 0.0061
Eisosomes 0.0001 0 0.0002 0 0.0006 0.0001 0.0001 0 0 0 0 0 0.0004 0 0 0.0003 0.0002 0
Endoplasmic Reticulum 0.0006 0 0.0004 0 0.0001 0.0003 0.0033 0 0.0004 0 0.0001 0.0002 0.002 0 0.0001 0.0021 0.0017 0.0001
Endosome 0.0025 0 0.0008 0 0.0013 0.0009 0.0287 0 0.008 0 0.0048 0.0013 0.0087 0 0.002 0.0011 0.0237 0.0005
Golgi 0.0022 0 0.0005 0 0.0028 0.0019 0.008 0 0.0041 0 0.002 0.0008 0.0038 0 0.0004 0.0003 0.0058 0.0001
Lipid Particles 0.0078 0 0.0028 0 0.0084 0.0013 0.0269 0 0.006 0 0.022 0.0022 0.008 0 0.0005 0.0012 0.0105 0.0015
Mitochondria 0.0009 0.0001 0.0029 0 0.0022 0.0003 0.007 0.0001 0.0052 0.0002 0.034 0.0054 0.0158 0 0.0017 0.0038 0.0509 0.0002
None 0.0008 0 0.0015 0 0.0003 0.0001 0.0014 0 0.0002 0 0.0001 0.0001 0.002 0 0.0002 0.0095 0.0108 0.0024
Nuclear Periphery 0.0048 0.0002 0.0015 0.0002 0.0012 0.0003 0.0125 0.0006 0.0005 0.0005 0.0036 0.0005 0.0071 0.0025 0.0006 0.0024 0.012 0.0009
Nucleolus 0.026 0.0212 0.0129 0.0039 0.0926 0.0602 0.0202 0.0097 0.0109 0.0169 0.1092 0.0688 0.0254 0.0081 0.0132 0.0126 0.1419 0.0919
Nucleus 0.9056 0.9781 0.9503 0.9957 0.8282 0.9218 0.8214 0.9893 0.9495 0.9821 0.769 0.9153 0.8946 0.9852 0.9621 0.9402 0.6989 0.8883
Peroxisomes 0.0051 0 0.0054 0 0.0151 0.0002 0.0138 0 0.0057 0 0.0255 0.0007 0.002 0 0.004 0.0006 0.0009 0.0001
Punctate Nuclear 0.0072 0.0001 0.0109 0.0001 0.0038 0.0002 0.0049 0 0.0003 0.0002 0.007 0.0002 0.0066 0.004 0.001 0.0034 0.0185 0.0062
Vacuole 0.0003 0 0.0009 0 0.0006 0.0001 0.0054 0.0001 0.001 0 0.0053 0.0006 0.0014 0 0.0026 0.0011 0.002 0.0001
Vacuole Periphery 0.0002 0 0.0003 0 0.0005 0.0001 0.0026 0.0001 0.0004 0 0.0014 0.0003 0.0025 0 0.002 0.0005 0.002 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 20.4818 22.5947 23.704 25.879 19.7754 21.3416 23.3379 26.8506 26.1582 22.7977
Translational Efficiency 1.0566 1.059 0.9025 0.8723 0.8861 0.9899 0.9574 0.7558 0.7687 0.7786

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
942 1564 847 929 187 1496 265 2 1129 3060 1112 931

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 706.88 904.68 1169.41 997.04 867.08 887.54 1136.07 901.16 733.41 896.30 1161.46 996.83
Standard Deviation 162.21 147.18 165.40 185.81 127.06 131.25 151.54 47.66 167.85 139.88 162.83 185.68
Intensity Change Log 2 — 0.355942 0.726244 0.496186 — 0.033647 0.389815 0.055618 — 0.187349 0.550668 0.270233

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000400 0.000393 0.000795 0.002745 0.000588 0.000837 0.001343 0.010715 0.000431 0.000610 0.000926 0.002763
Bud Neck 0.004616 0.009211 0.018228 0.028991 0.002334 0.008636 0.013802 0.014331 0.004238 0.008930 0.017173 0.028960
Bud Site 0.000833 0.000518 0.001020 0.009221 0.000319 0.000902 0.001420 0.013165 0.000747 0.000706 0.001115 0.009229
Cell Periphery 0.000092 0.000074 0.000036 0.000506 0.000026 0.000055 0.000044 0.000455 0.000081 0.000065 0.000038 0.000506
Cytoplasm 0.005312 0.000408 0.000111 0.007778 0.000169 0.000861 0.000283 0.061751 0.004460 0.000630 0.000152 0.007894
Cytoplasmic Foci 0.001083 0.000032 0.000023 0.007651 0.000118 0.001473 0.000022 0.002845 0.000923 0.000737 0.000023 0.007640
Eisosomes 0.000020 0.000028 0.000056 0.000074 0.000026 0.000033 0.000059 0.000045 0.000021 0.000030 0.000057 0.000074
Endoplasmic Reticulum 0.002337 0.002485 0.001213 0.004430 0.003233 0.003666 0.003842 0.006912 0.002485 0.003062 0.001840 0.004436
Endosome 0.000232 0.000133 0.000035 0.011958 0.000102 0.000856 0.000099 0.000859 0.000211 0.000486 0.000051 0.011934
Golgi 0.000092 0.000063 0.000070 0.007420 0.000148 0.000590 0.000094 0.005876 0.000101 0.000321 0.000076 0.007417
Lipid Particles 0.000394 0.000025 0.000021 0.002401 0.000105 0.000487 0.000080 0.000606 0.000346 0.000251 0.000035 0.002397
Mitochondria 0.002067 0.000633 0.001645 0.010306 0.000498 0.002510 0.001654 0.005598 0.001807 0.001551 0.001647 0.010296
Mitotic Spindle 0.002119 0.000204 0.000104 0.029979 0.000579 0.007157 0.000243 0.010447 0.001864 0.003603 0.000137 0.029937
None 0.008466 0.001656 0.000766 0.010375 0.001380 0.001748 0.001405 0.007242 0.007292 0.001701 0.000918 0.010368
Nuclear Periphery 0.000591 0.000511 0.000096 0.002725 0.000055 0.000800 0.000192 0.000975 0.000502 0.000652 0.000119 0.002722
Nuclear Periphery Foci 0.000481 0.000614 0.000012 0.002718 0.000353 0.000849 0.000049 0.005859 0.000460 0.000729 0.000021 0.002725
Nucleolus 0.044240 0.023252 0.032210 0.019592 0.039458 0.045424 0.019346 0.066030 0.043448 0.034092 0.029144 0.019692
Nucleus 0.925355 0.958815 0.941917 0.818765 0.949274 0.918993 0.953988 0.769557 0.929316 0.939346 0.944793 0.818659
Peroxisomes 0.000605 0.000231 0.001032 0.002981 0.000768 0.001016 0.000891 0.004189 0.000632 0.000615 0.000998 0.002983
Vacuole 0.000527 0.000256 0.000403 0.013080 0.000381 0.001773 0.001013 0.006695 0.000503 0.000998 0.000549 0.013066
Vacuole Periphery 0.000138 0.000458 0.000207 0.006304 0.000086 0.001333 0.000131 0.005847 0.000129 0.000885 0.000189 0.006303

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 0.67 -8.56 -2.82 -2.86 -2.16 NAN NAN NAN NAN NAN -1.91 -11.60 -2.80 -2.56 -1.92
Bud Neck -5.67 -12.03 -10.47 -8.31 -3.89 NAN NAN NAN NAN NAN -8.26 -14.58 -10.68 -8.67 -4.17
Bud Site 1.96 -1.46 -6.59 -7.06 -6.33 NAN NAN NAN NAN NAN 0.60 -3.37 -6.73 -6.89 -6.15
Cell Periphery 1.01 4.12 -1.74 -1.88 -2.12 NAN NAN NAN NAN NAN 1.31 3.66 -1.82 -1.95 -2.11
Cytoplasm 4.44 4.97 -0.98 -6.26 -6.92 NAN NAN NAN NAN NAN 4.22 4.92 -1.81 -6.29 -6.96
Cytoplasmic Foci 2.67 2.69 -2.83 -4.50 -4.51 NAN NAN NAN NAN NAN 1.17 2.73 -3.11 -3.84 -4.52
Eisosomes -2.94 -12.55 -3.67 -3.18 -0.55 NAN NAN NAN NAN NAN -4.88 -15.30 -3.60 -2.96 -0.39
Endoplasmic Reticulum -0.43 6.15 -6.15 -6.10 -10.17 NAN NAN NAN NAN NAN -2.53 3.25 -5.85 -4.31 -7.93
Endosome 2.81 6.39 -5.16 -5.25 -5.32 NAN NAN NAN NAN NAN -2.34 5.66 -5.18 -4.98 -5.31
Golgi 2.19 1.57 -3.64 -3.67 -3.66 NAN NAN NAN NAN NAN -2.04 0.80 -3.64 -3.46 -3.66
Lipid Particles 3.75 3.75 -2.15 -3.12 -3.12 NAN NAN NAN NAN NAN 1.48 3.72 -2.28 -2.67 -3.09
Mitochondria 1.55 0.33 -3.11 -4.73 -3.92 NAN NAN NAN NAN NAN 0.32 0.01 -3.43 -4.00 -3.91
Mitotic Spindle 1.88 1.89 -6.36 -7.17 -7.17 NAN NAN NAN NAN NAN -1.33 1.83 -6.49 -6.09 -7.14
None 5.85 6.66 0.48 -3.89 -4.46 NAN NAN NAN NAN NAN 5.57 6.56 -0.15 -3.78 -4.37
Nuclear Periphery 0.31 2.45 -3.03 -3.10 -4.25 NAN NAN NAN NAN NAN -0.50 2.19 -3.27 -2.90 -4.19
Nuclear Periphery Foci -0.21 3.48 -3.24 -2.64 -4.63 NAN NAN NAN NAN NAN -0.95 3.44 -3.28 -2.45 -4.63
Nucleolus 7.43 3.03 7.38 0.79 4.38 NAN NAN NAN NAN NAN 4.03 4.28 7.67 5.82 3.72
Nucleus -7.84 -2.96 7.85 12.71 9.96 NAN NAN NAN NAN NAN -3.03 -2.97 8.54 10.49 10.42
Peroxisomes 1.89 -2.15 -2.37 -3.22 -1.65 NAN NAN NAN NAN NAN 0.34 -1.95 -2.33 -2.55 -1.75
Vacuole 0.99 0.26 -6.09 -6.31 -6.20 NAN NAN NAN NAN NAN -1.25 -0.68 -6.12 -5.79 -6.08
Vacuole Periphery -1.35 -2.84 -4.16 -3.70 -4.08 NAN NAN NAN NAN NAN -2.75 -3.18 -4.18 -3.22 -4.11
Protein Complexes26S Proteasome complex37S mitochondrial small ribosomal subunit54S mitochondrial large ribosomal subunit60S cytosolic large ribosomal subunitAar2-U5 snRNPAnaphase-Promoting Complex variant 1Anaphase-Promoting Complex variant 2Anaphase-Promoting Complex variant 3Anaphase-Promoting Complex variant 4Arp2/3 complexATG1 kinase complexBLOC-1 complexCcr4-Not complexCentral kinetochore CTF19 complexCleavage and polyadenylation specificity factorcomplexCMG helicase complexCMG-Pol epsilon complexCOMPASS complexCOP9 signalosome COPI vesicle coat complexCoQ6 biosynthesis complexCore mediator complexCORVET complexCTF18-RFC complexCURI complex variant 1CURI complex variant 2CURI complex variant 3Cytoplasmic exosome complex, DIS3 variantDASH complexDNA-directed RNA Polymerase I complexDNA-directed RNA polymerase II complexDNA-directed RNA polymerase III complexDNA replication factor C complexEGO complexELG1-RFC complex Elongator holoenzyme complexEndoplasmic Reticulum Membrane ComplexEukaryotic translation initiation factor 2BcomplexEukaryotic translation initiation factor 3core complexExocystExomer complexFAR complexGeneral transcription factor complex TFIIDGeneral transcription factor complex TFIIHGET4-GET5 transmembrane domain recognition complexGID ubiquitin ligase complexGin4 complexGolgi transport complexGSE complexHMC complexHOPS complexINO80 chromatin remodeling complexLSM1-7 complexLsm1-7-Pat1 complexLSM2-7 complexLSM2-8 complexMCM complexMICOS complexMitochondrial electron transport complex IIIMitochondrial inner membrane pre-sequence translocasecomplexMitochondrial outer membrane translocase holocomplexMitochondrial proton-transporting ATP synthase complexMitochondrial pyruvate dehydrogenase complexNineTeen complexNOP8 60s ribosome pre-assembly complexNuA3 histone acetyltransferase complexNuA4 histone acetyltransferase complexNuclear condensin complexNuclear/nucleolar exosome complex, DIS3-RRP6 variantNuclear pore complexNucleolar ribonuclease MRP complexNucleolar ribonuclease P complexOligosaccharyl transferase complex variant 1Oligosaccharyl transferase complex variant 2PAF1 complexPAS complexPhosphatidylinositol 3-kinase complex IPolarisomePrefoldin complexPRP19-associated complexRad17 RFC-like complexRetromer complexRPD3L histone deacetylase complexRPD3S histone deacetylase complexRQC complexRSC complex variant RSC1RSC complex variant RSC2SAGA complexSCF-Met30 ubiquitin ligase complexSCF-Ufo1 ubiquitin ligase complexSEA complexSeptin complexSET3C histone deacetylase complexSignal recognition particleSLIK (SAGA-like) complexSmall ribosomal subunit processome, variant1Small ribosomal subunit processome, variant2Small ribosomal subunit processome, variant3SMC5-SMC6 SUMO ligase complexSm complexSpliceosomal commitment complexSPOTS complexSWI/SNF chromatin remodelling complexSwr1 chromatin remodelling complexTHO complexTranscription factor TFIIIC complexTranslocon complexTRAPPIII protein complexTRAPPII protein complexTRAPPI protein complexTREX complexU1 snRNPU2 snRNPU4 snRNPU4/U6 snRNPU4/U6.U5 tri-snRNP complexU5 snRNPU6 snRNP complexUTP-A complexUTP-B complexUTP-C complex variant 1UTP-C complex variant 2UTP-C complex variant 3Vacuolar proton translocating ATPase complex,Golgi variantVacuolar proton translocating ATPase complex,vacuole variantCellular Components••••••••••••••••actinbud neckcell peripherycytoplasmendosomeERGolgimitochondrionmixednuclear peripherynucleolusnucleusperoxisomespindle polevacuolar membranevacuole
Standard name
Human Ortholog
Description Component of RNA polymerase transcription factor TFIIH holoenzyme; acts as dsDNA-dependent translocase in context of TFIIH, unwinds DNA strands during initiation and promotes transcription start site (TSS) scanning; has DNA-dependent ATPase/helicase activity; interacts functionally with TFIIB, has roles in TSS selection and gene looping to juxtapose initiation and termination regions; involved in DNA repair; relocalizes to cytosol under hypoxia; homolog of human ERCC3
Localization
Cell Percentages nucleus (99%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-4

Ssl2

Ssl2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ssl2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available