Standard name
Human Ortholog
Description Component of the kinetochore-associated Ndc80 complex; conserved coiled-coil protein involved in chromosome segregation, spindle checkpoint activity, and kinetochore assembly and clustering; evolutionarily conserved; complex members include Ndc80p, Nuf2p, Scp24p, and Spc25p; modified by sumoylation
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0.14 0.2 0.06 0.07 0.07 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0.08 0 0 0 0 0 0 0 0 0 0 0 0.12 0.17 0.08 0.11 0.09 0 0 0 0 0.06 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0.09 0 0 0 0 0.06 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0.05 0.08 0.08 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.51 0.52 0.34 0.27 0.37 0.39 0.4 0.35 0.37 0.39 0.35 0.44 0.56 0.45 0.44 0.45 0.47 0.48 0 0.06 0 0 0 0
Peroxisomes 0.05 0.06 0 0 0 0 0 0 0 0 0.05 0 0 0 0.07 0 0 0 0 0 0 0 0 0
SpindlePole 0.89 0.85 0.93 0.91 0.88 0.87 0.82 0.87 0.87 0.86 0.78 0.84 0.83 0.73 0.65 0.81 0.8 0.83 0.92 0.84 0.89 0.87 0.82 0.85
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 1 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 2 2 0 0 1 0
Bud 7 4 2 3 4 3 5 18 11 12 9 5 0 0 0 9 4 3 2 4 1 2 2 7
Bud Neck 8 6 11 4 8 12 8 10 11 15 11 5 1 3 3 3 4 2 0 5 3 0 0 3
Bud Site 11 3 2 3 1 9 13 12 15 20 12 8 3 1 1 10 5 9 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0
Cytoplasm 6 13 12 7 14 13 13 17 10 11 9 8 17 45 80 35 29 26 0 1 0 1 2 3
Endoplasmic Reticulum 0 0 0 1 0 0 0 0 0 0 0 1 0 0 0 5 4 2 0 0 1 0 0 1
Endosome 16 44 9 7 10 13 8 10 12 6 7 15 7 41 69 45 44 31 16 18 9 9 19 10
Golgi 0 0 1 0 0 0 0 0 0 0 1 0 0 0 1 1 0 2 3 4 2 0 1 0
Mitochondria 16 5 0 3 1 5 8 4 8 12 29 7 2 0 1 30 13 11 2 3 0 0 4 3
Nucleus 14 14 4 8 16 12 16 19 10 11 12 11 4 5 15 29 30 30 0 1 0 2 4 3
Nuclear Periphery 0 1 0 1 0 0 1 0 2 0 0 1 0 0 0 0 1 3 0 1 0 0 1 1
Nucleolus 300 302 136 68 137 159 135 190 149 185 112 154 120 149 180 245 183 170 10 34 13 3 12 7
Peroxisomes 28 32 5 3 5 11 3 9 16 8 16 9 0 8 30 19 14 6 5 5 3 3 1 3
SpindlePole 519 495 371 231 326 357 280 468 354 405 250 293 177 243 266 435 313 292 554 505 365 192 270 296
Vac/Vac Membrane 0 5 1 1 0 0 0 1 1 0 0 0 1 2 3 10 4 4 0 1 0 1 2 3
Unique Cell Count 586 579 398 254 371 410 341 541 408 472 319 348 214 332 410 539 391 353 604 603 411 222 329 350
Labelled Cell Count 926 924 554 340 522 594 490 758 599 686 468 517 332 497 650 876 648 591 604 603 411 222 329 350


SpindlePole

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.5 7.7 7.1 6.5 7.0 6.1 6.3 6.5 5.6 5.9 5.3 5.7 7.1 7.1 6.9 7.5 7.7 7.1 6.7 6.8 6.8
Std Deviation (1e-4) 1.0 1.3 1.1 1.3 1.6 1.6 1.5 1.4 1.4 1.6 1.8 1.3 1.3 1.6 1.7 1.7 1.7 1.6 1.4 1.5 1.4
Intensity Change (Log2) – – – -0.13 -0.03 -0.23 -0.18 -0.12 -0.34 -0.27 -0.43 -0.32 0.01 -0.0 -0.05 0.07 0.11 -0.01 -0.09 -0.06 -0.06


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 3.0 2.5 2.4 0 0 0 0 0 0 0 0
Bud Neck -1.0 -0.5 0.1 -0.4 -0.9 -0.1 0.4 0.5 -1.2 0 -1.8 -2.2 -2.8 -1.8 -2.3
Bud Site 0 0 2.1 3.2 2.1 3.1 3.5 3.1 0 0 0 0 1.8 0 2.3
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm -0.2 0.6 0.1 0.6 0.1 -0.5 -0.6 -0.2 -0.6 2.7 5.3 7.4 2.4 2.8 2.7
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0.4 0.4 0.8 0.1 -0.4 0.6 -1.1 -0.1 1.6 0.7 5.4 7.0 4.0 5.0 4.0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 3.2 6.1 0 0 0 0 4.8 3.7 3.5
Nucleus 0 2.9 2.0 3.1 2.5 1.6 1.5 2.5 2.1 0 0 2.5 3.6 4.6 4.9
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus -2.0 0.8 1.4 1.5 0.3 0.7 1.5 0.3 2.8 5.2 3.0 2.8 3.5 3.6 3.9
Peroxisomes 0 0 1.5 0 0.5 2.4 0.5 3.0 1.3 0 1.2 4.2 2.2 2.1 0.5
SpindlePole -1.1 -2.5 -2.9 -4.6 -3.3 -3.0 -3.5 -5.8 -3.9 -4.1 -7.4 -9.9 -5.5 -5.4 -4.5
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 2.5416 2.1499 2.4069 2.1523 2.3814 2.2296 2.1193 1.9037 2.1271 2.4042 1.9666 2.2316 2.4232 2.3341 2.1365 2.4415 2.2978 2.3986
Actin 0.0112 0.0003 0.002 0.0001 0.0154 0.0014 0.0111 0.0014 0.0021 0.0032 0.0001 0 0 0 0 0 0 0
Bud 0.0006 0.0027 0.0001 0 0.0003 0.0004 0.0004 0.0005 0.0004 0.0055 0 0 0 0 0 0 0 0
Bud Neck 0.003 0.0021 0.0004 0.0008 0.0003 0.0003 0.0013 0.0005 0.0007 0.0002 0.0001 0.0001 0.0001 0.0005 0.0002 0.0001 0.0013 0.0002
Bud Periphery 0.0007 0.0005 0.0001 0 0.0004 0.001 0.0003 0.0002 0.0003 0.0109 0 0 0 0 0 0 0 0
Bud Site 0.0088 0.0107 0.0008 0.0001 0.0008 0.0003 0.0038 0.001 0.0059 0.0161 0 0 0.0001 0.0006 0.0001 0 0.0003 0
Cell Periphery 0.0003 0.0002 0 0 0.0001 0 0.0001 0 0.0001 0.0004 0 0 0 0 0 0 0 0
Cytoplasm 0.0029 0.0058 0.0006 0.0004 0.0009 0.0008 0.0093 0.0002 0.0102 0.0041 0 0 0 0 0 0 0.0001 0
Cytoplasmic Foci 0.0346 0.0151 0.0106 0.0125 0.0767 0.0049 0.0163 0.0027 0.0099 0.0219 0.0085 0.0037 0.0157 0.0019 0.0018 0.0064 0.0319 0.0009
Eisosomes 0.0004 0 0 0 0.0001 0 0.0001 0 0 0.0001 0 0 0 0 0 0 0 0
Endoplasmic Reticulum 0.0008 0.0001 0.0009 0.0001 0.0013 0.0008 0.0012 0.0001 0.0009 0.0005 0 0 0 0 0 0 0 0
Endosome 0.0138 0.0013 0.0054 0.0262 0.0257 0.0081 0.017 0.0011 0.0105 0.0527 0.0005 0.0001 0.0001 0 0 0 0.0021 0
Golgi 0.0069 0.0005 0.0016 0.0061 0.0081 0.0024 0.0043 0.002 0.0024 0.0199 0.0019 0.0001 0.0001 0.0001 0 0.0001 0.0023 0
Lipid Particles 0.0469 0.0012 0.0123 0.0034 0.0546 0.0073 0.0172 0.0005 0.0078 0.043 0.0739 0.0017 0.0004 0.0001 0.0005 0.0006 0.0264 0.0002
Mitochondria 0.0283 0.0005 0.0011 0.0016 0.0084 0.0071 0.0032 0.0029 0.0015 0.0264 0.0001 0 0 0 0 0 0 0
None 0.0102 0.0016 0.0004 0.0001 0.0003 0.0003 0.0052 0.0001 0.0011 0.0012 0 0 0 0 0 0 0.0001 0
Nuclear Periphery 0.0056 0.0013 0.0022 0.0003 0.0006 0.0037 0.0051 0.0003 0.0028 0.0009 0.0002 0.0002 0.0001 0.0001 0 0.0003 0.0007 0
Nucleolus 0.0464 0.1003 0.068 0.0474 0.0109 0.0331 0.0525 0.0768 0.0405 0.0015 0.0025 0.0093 0.0296 0.0519 0.0378 0.0028 0.0235 0.0152
Nucleus 0.0317 0.1055 0.0362 0.0158 0.0008 0.0154 0.0319 0.0435 0.0191 0.001 0.0003 0.0014 0.0034 0.0238 0.0134 0.0008 0.0009 0.0087
Peroxisomes 0.0102 0.0005 0.0106 0.0005 0.003 0.0014 0.0099 0.0001 0.0019 0.006 0.0017 0.0001 0.0001 0 0.0001 0.0002 0.0057 0
Punctate Nuclear 0.7337 0.7487 0.8461 0.8838 0.79 0.909 0.8075 0.8652 0.8806 0.7804 0.9101 0.9832 0.9503 0.9207 0.9459 0.9885 0.9046 0.9745
Vacuole 0.0021 0.0009 0.0003 0.0004 0.0009 0.001 0.0017 0.0003 0.0009 0.0024 0 0 0 0 0 0 0.0001 0
Vacuole Periphery 0.0009 0.0001 0.0002 0.0002 0.0003 0.0013 0.0007 0.0003 0.0003 0.0017 0 0 0 0 0 0 0 0

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 14.6164 14.254 6.6988 10.5291 15.1606 13.8115 16.4539 13.9424 11.5937 19.1303
Translational Efficiency 1.3899 1.3498 1.2965 0.8364 1.145 1.3047 1.0178 0.9459 1.0088 0.923

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1788 1443 1771 1107 1395 2555 837 2370 3183 3998 2608 3477

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 732.35 819.30 928.79 937.62 751.80 783.73 935.88 927.63 740.87 796.57 931.07 930.81
Standard Deviation 91.22 125.43 122.94 138.69 95.02 114.01 124.87 151.33 93.40 119.49 123.61 147.49
Intensity Change Log 2 — 0.161859 0.342819 0.356470 — 0.060008 0.315975 0.303201 — 0.111165 0.329283 0.329732

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000177 0.000342 0.000049 0.001688 0.000173 0.000295 0.000106 0.000179 0.000175 0.000312 0.000067 0.000659
Bud Neck 0.001748 0.002553 0.000787 0.003343 0.001884 0.004334 0.001308 0.002603 0.001808 0.003691 0.000954 0.002838
Bud Site 0.002651 0.003606 0.002053 0.019683 0.002570 0.005385 0.002302 0.009611 0.002615 0.004743 0.002133 0.012818
Cell Periphery 0.000106 0.000122 0.000034 0.000059 0.000065 0.000124 0.000049 0.000045 0.000088 0.000123 0.000038 0.000049
Cytoplasm 0.004413 0.007433 0.000819 0.009258 0.004967 0.006568 0.008303 0.008117 0.004656 0.006880 0.003221 0.008480
Cytoplasmic Foci 0.027980 0.017989 0.001338 0.014776 0.012419 0.025046 0.007896 0.011532 0.021160 0.022499 0.003443 0.012565
Eisosomes 0.000212 0.000061 0.000006 0.000010 0.000090 0.000059 0.000014 0.000010 0.000159 0.000060 0.000009 0.000010
Endoplasmic Reticulum 0.001616 0.001187 0.000139 0.000550 0.001127 0.001779 0.000343 0.000135 0.001402 0.001565 0.000204 0.000267
Endosome 0.002867 0.002430 0.000196 0.003837 0.002068 0.005426 0.001255 0.001019 0.002517 0.004345 0.000536 0.001916
Golgi 0.005867 0.003541 0.000226 0.004570 0.003810 0.006169 0.000556 0.001072 0.004965 0.005220 0.000332 0.002186
Lipid Particles 0.004241 0.002235 0.000086 0.001148 0.001628 0.006282 0.000358 0.001142 0.003096 0.004821 0.000173 0.001144
Mitochondria 0.001122 0.000492 0.000082 0.004463 0.000344 0.002504 0.000155 0.001147 0.000781 0.001778 0.000105 0.002203
Mitotic Spindle 0.746879* 0.828789* 0.972972* 0.912496* 0.797728* 0.791397* 0.934955* 0.943852* 0.769164* 0.804893* 0.960771* 0.933869*
None 0.003029 0.004779 0.000240 0.001654 0.002648 0.002655 0.000859 0.001022 0.002862 0.003422 0.000439 0.001223
Nuclear Periphery 0.000375 0.000337 0.000101 0.000564 0.000281 0.000989 0.000406 0.000141 0.000334 0.000754 0.000199 0.000276
Nuclear Periphery Foci 0.048689 0.033594 0.001765 0.002857 0.054031 0.044690 0.003898 0.003401 0.051030 0.040685 0.002450 0.003227
Nucleolus 0.090760 0.054540 0.011719 0.002059 0.078015 0.057790 0.010245 0.005203 0.085174 0.056617 0.011246 0.004202
Nucleus 0.021814 0.024272 0.005912 0.002506 0.023128 0.025674 0.020670 0.004865 0.022390 0.025168 0.010648 0.004114
Peroxisomes 0.034406 0.010701 0.001115 0.004171 0.012464 0.010700 0.002663 0.003916 0.024790 0.010701 0.001612 0.003997
Vacuole 0.000743 0.000788 0.000332 0.008407 0.000475 0.001423 0.003494 0.000877 0.000626 0.001194 0.001347 0.003274
Vacuole Periphery 0.000306 0.000208 0.000029 0.001906 0.000083 0.000713 0.000166 0.000112 0.000208 0.000531 0.000073 0.000683

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -3.66 1.14 -2.48 -2.05 -2.54 -2.20 -3.76 -1.71 -0.04 2.51 -4.45 -1.94 -2.75 -1.99 -2.40
Bud Neck -4.73 -2.14 -6.80 -4.18 -5.49 -5.55 -4.26 -5.79 -2.16 -1.76 -7.74 -4.01 -8.81 -4.09 -5.68
Bud Site -4.39 -0.50 -8.99 -8.00 -8.66 -4.44 -4.86 -8.78 -6.91 -3.84 -6.36 -3.17 -12.60 -10.51 -10.26
Cell Periphery -0.93 4.50 3.42 3.64 -2.16 -3.88 -0.43 2.58 5.83 2.15 -2.55 3.84 5.19 6.94 -0.79
Cytoplasm -2.38 3.47 -4.95 -3.76 -5.88 -1.62 -3.24 -4.38 -3.42 -0.06 -2.71 -0.83 -6.67 -4.99 -5.73
Cytoplasmic Foci 0.90 11.80 2.88 1.85 -7.41 -5.52 1.60 -1.36 4.08 -2.69 -2.18 11.33 2.19 4.38 -9.09
Eisosomes 12.80 19.88 19.74 16.20 -0.60 7.64 14.80 21.98 20.94 5.74 14.79 24.54 25.99 26.49 4.17
Endoplasmic Reticulum 2.02 10.81 4.83 3.57 -1.82 0.33 2.77 15.88 6.26 5.93 1.54 11.29 13.08 7.53 2.46
Endosome 0.32 5.20 -0.23 -0.54 -4.87 -2.96 2.63 5.27 6.93 1.36 -1.87 6.56 2.96 5.17 -3.94
Golgi 5.58 18.48 6.49 3.12 -2.64 0.82 14.65 13.81 9.90 -0.07 4.52 23.78 14.80 9.62 -3.25
Lipid Particles 3.20 7.37 4.69 1.89 -3.44 -3.07 4.61 2.60 4.94 -1.42 -0.30 8.86 5.37 5.26 -3.72
Mitochondria 0.65 1.31 -1.85 -2.21 -2.45 -3.28 -0.83 -1.27 0.96 -1.16 -1.64 1.29 -1.88 -0.89 -2.74
Mitotic Spindle -5.39 -25.24* -14.89 -9.21 7.13 -3.17 -11.02 -18.07 -19.26 -4.08 -6.46 -27.24 -25.56 -21.05 2.86
None -1.08 7.11 2.43 2.87 -1.59 0.79 3.86 3.67 3.33 0.03 0.10 8.34 4.80 4.42 -2.13
Nuclear Periphery -0.64 2.95 -2.39 -2.25 -3.47 -3.07 -2.38 3.92 3.89 3.02 -3.04 -0.51 -0.69 2.37 -0.08
Nuclear Periphery Foci 3.47 19.55 19.19* 14.65 0.18 3.22 15.47 17.78 20.37* 3.00 4.13 25.19 26.26 25.05* 1.70
Nucleolus 4.66 15.03 22.00* 16.76 9.40 5.86 11.77 16.71 16.61 4.73 8.01 19.11 26.67 23.09 8.75
Nucleus -1.99 2.50 2.59 4.03 0.28 0.18 -2.77 6.10 6.79 5.65 -1.07 -0.09 6.93 7.95 5.10
Peroxisomes 10.19 18.35 16.58 8.75 -2.32 3.45 8.86 10.70 8.99 0.57 11.27 20.70 19.50 12.44 -2.03
Vacuole -1.58 -1.11 -6.54 -6.44 -6.42 -2.77 -3.88 -4.05 -3.07 3.05 -2.94 -4.02 -7.44 -7.05 -3.02
Vacuole Periphery 0.29 1.29 -2.70 -2.87 -3.15 -1.91 -1.87 -1.58 1.63 1.04 -1.37 0.68 -2.49 -1.06 -3.06
Standard name
Human Ortholog
Description Component of the kinetochore-associated Ndc80 complex; conserved coiled-coil protein involved in chromosome segregation, spindle checkpoint activity, and kinetochore assembly and clustering; evolutionarily conserved; complex members include Ndc80p, Nuf2p, Scp24p, and Spc25p; modified by sumoylation
Localization
Cell Percentages spindle pole (82%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Ndc80

Ndc80


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ndc80-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available