Standard name | |
---|---|
Human Ortholog | |
Description | Essential chromatin-associated protein; involved in initiation of DNA replication; required for association of MCM2-7 complex with replication origins; required to stabilize catalytic subunit of DNA polymerase-alpha; self-associates through its N-terminal domain |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 1 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 3 | 0 | 1 | 1 | 3 | 3 | 1 | 7 | 1 | 0 | 0 | 0 | 2 | 1 | 1 | 1 | 3 | 3 | 0 | 1 | 4 |
Bud Neck | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 6 | 2 | 0 | 2 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 2 | 0 | 1 | 3 |
Bud Site | 1 | 0 | 0 | 1 | 0 | 0 | 2 | 2 | 5 | 3 | 6 | 3 | 0 | 0 | 0 | 1 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 6 | 15 | 16 | 24 | 41 | 49 | 76 | 67 | 40 | 59 | 26 | 40 | 25 | 47 | 80 | 25 | 22 | 37 | 2 | 3 | 5 | 1 | 10 | 8 |
Endoplasmic Reticulum | 3 | 0 | 0 | 1 | 2 | 2 | 3 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 12 | 10 | 8 | 0 | 0 | 1 | 0 | 0 | 0 |
Endosome | 0 | 1 | 1 | 0 | 2 | 8 | 8 | 3 | 0 | 1 | 0 | 2 | 3 | 8 | 9 | 9 | 10 | 10 | 2 | 0 | 2 | 1 | 3 | 5 |
Golgi | 0 | 0 | 0 | 1 | 1 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 |
Mitochondria | 24 | 10 | 1 | 23 | 7 | 71 | 85 | 43 | 118 | 81 | 200 | 139 | 1 | 0 | 2 | 25 | 11 | 16 | 1 | 4 | 1 | 0 | 3 | 8 |
Nucleus | 187 | 144 | 188 | 188 | 202 | 197 | 184 | 197 | 123 | 116 | 118 | 132 | 185 | 172 | 215 | 164 | 163 | 163 | 182 | 150 | 171 | 34 | 89 | 65 |
Nuclear Periphery | 1 | 1 | 0 | 1 | 1 | 0 | 2 | 0 | 2 | 1 | 0 | 1 | 0 | 0 | 0 | 4 | 2 | 0 | 2 | 0 | 0 | 0 | 0 | 2 |
Nucleolus | 0 | 0 | 0 | 2 | 1 | 3 | 1 | 1 | 1 | 2 | 2 | 1 | 2 | 4 | 1 | 8 | 11 | 8 | 6 | 4 | 5 | 0 | 18 | 9 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 1 | 1 | 2 | 9 | 7 | 7 | 11 | 13 | 7 | 7 | 2 | 10 | 11 | 9 | 10 | 4 | 4 | 5 | 1 | 3 | 8 | 1 | 15 | 10 |
Vac/Vac Membrane | 15 | 28 | 14 | 5 | 25 | 46 | 39 | 23 | 16 | 18 | 14 | 26 | 5 | 11 | 17 | 28 | 30 | 32 | 24 | 21 | 48 | 8 | 50 | 51 |
Unique Cell Count | 215 | 175 | 215 | 240 | 273 | 329 | 352 | 325 | 233 | 226 | 270 | 277 | 216 | 238 | 314 | 236 | 228 | 250 | 234 | 194 | 256 | 51 | 198 | 179 |
Labelled Cell Count | 238 | 200 | 223 | 258 | 290 | 385 | 413 | 360 | 318 | 290 | 377 | 356 | 233 | 251 | 335 | 282 | 265 | 281 | 234 | 194 | 256 | 51 | 198 | 179 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 4.4 | 5.2 | 5.8 | 5.4 | 5.9 | 4.7 | 4.7 | 5.0 | 4.1 | 4.5 | 3.7 | 4.2 | 6.2 | 5.9 | 5.6 | 7.1 | 7.3 | 6.8 | 4.6 | 5.6 | 6.1 |
Std Deviation (1e-4) | 0.6 | 1.1 | 0.8 | 1.1 | 1.4 | 1.2 | 1.2 | 1.1 | 0.9 | 1.5 | 1.0 | 1.2 | 1.3 | 1.9 | 1.3 | 1.4 | 1.7 | 1.6 | 0.7 | 1.2 | 1.4 |
Intensity Change (Log2) | – | – | – | -0.12 | 0.01 | -0.3 | -0.32 | -0.22 | -0.53 | -0.37 | -0.66 | -0.49 | 0.09 | 0.02 | -0.06 | 0.28 | 0.32 | 0.21 | -0.34 | -0.05 | 0.06 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 1.0 | 2.6 | 2.6 | 4.4 | 4.2 | 3.1 | 5.2 | 0.9 | 2.4 | 1.5 | 3.8 | 5.3 | 1.2 | 0.8 | 2.5 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3.4 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2.7 | 2.5 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 4.3 | 0 | 0 | 0 | 5.3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 4.6 | 2.8 | 3.4 |
Nucleus | -2.6 | -3.7 | -6.9 | -8.6 | -6.7 | -8.0 | -8.2 | -9.9 | -9.2 | -0.5 | -4.0 | -5.0 | -4.6 | -4.1 | -5.6 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3.3 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 2.0 | 0 | 0 | 0 | 2.1 | 0 | 0 | 0 | 0 | 2.5 | 2.0 | 0 | 0 | 0 | 0 |
Vacuole | -2.4 | 1.1 | 2.7 | 1.8 | 0.3 | 0.2 | 0.6 | -0.6 | 1.2 | -2.1 | -0.9 | -0.5 | 2.0 | 2.3 | 2.3 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 1.858 | 1.6967 | 1.3575 | 1.4082 | 1.6614 | 1.4936 | 0.9416 | 0.5896 | 0.8111 | 0.7077 | 1.338 | 0.5907 | 1.1077 | 1.072 | 0.687 | 0.9765 | 0.8466 | 0.7175 |
Actin | 0.0074 | 0.0005 | 0.0076 | 0.0013 | 0.0055 | 0.0013 | 0.0372 | 0 | 0.0145 | 0.006 | 0.0086 | 0.0009 | 0.0389 | 0.0046 | 0.0069 | 0.0099 | 0 | 0.0076 |
Bud | 0.0009 | 0.0001 | 0.0013 | 0.0001 | 0.0004 | 0.0001 | 0.0006 | 0 | 0.0006 | 0.001 | 0.0009 | 0.0002 | 0.0052 | 0.0021 | 0.0004 | 0.0002 | 0 | 0.0003 |
Bud Neck | 0.0128 | 0.0003 | 0.0012 | 0.0003 | 0.002 | 0.0017 | 0.0033 | 0.0003 | 0.002 | 0.0029 | 0.0015 | 0.0032 | 0.01 | 0.0006 | 0.0007 | 0.0008 | 0.0002 | 0.0027 |
Bud Periphery | 0.0015 | 0.0001 | 0.0017 | 0.0001 | 0.0004 | 0.0003 | 0.0012 | 0.0001 | 0.0011 | 0.0025 | 0.0016 | 0.0004 | 0.0025 | 0.0007 | 0.0004 | 0.0004 | 0 | 0.0009 |
Bud Site | 0.01 | 0.0002 | 0.0106 | 0.0001 | 0.0014 | 0.0004 | 0.0046 | 0.0003 | 0.006 | 0.0037 | 0.0024 | 0.001 | 0.028 | 0.0033 | 0.0036 | 0.0017 | 0.0001 | 0.0006 |
Cell Periphery | 0.0005 | 0 | 0.0002 | 0 | 0.0001 | 0.0001 | 0.0005 | 0 | 0.0004 | 0.0016 | 0.0002 | 0.0001 | 0.0005 | 0.0001 | 0.0001 | 0.0001 | 0 | 0.0003 |
Cytoplasm | 0.0155 | 0.0029 | 0.0046 | 0.0006 | 0.01 | 0.0008 | 0.0212 | 0.0095 | 0.0069 | 0.0042 | 0.0062 | 0.0016 | 0.0246 | 0.0084 | 0.0091 | 0.0018 | 0.003 | 0.013 |
Cytoplasmic Foci | 0.0179 | 0.0015 | 0.0056 | 0.0031 | 0.0332 | 0.0034 | 0.0175 | 0.0002 | 0.0048 | 0.0049 | 0.0321 | 0.0011 | 0.0194 | 0.0006 | 0.0103 | 0.0019 | 0 | 0.0026 |
Eisosomes | 0.0002 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0.0007 | 0 | 0.0002 | 0.0001 | 0.0001 | 0 | 0.0005 | 0 | 0 | 0.0002 | 0 | 0.0008 |
Endoplasmic Reticulum | 0.0034 | 0.0002 | 0.0006 | 0.0003 | 0.007 | 0.0012 | 0.0094 | 0.0005 | 0.0043 | 0.0027 | 0.0035 | 0.0002 | 0.0137 | 0.0008 | 0.0006 | 0.0001 | 0.0001 | 0.0003 |
Endosome | 0.0372 | 0.0015 | 0.013 | 0.0198 | 0.1022 | 0.0124 | 0.0316 | 0.0009 | 0.015 | 0.0235 | 0.0319 | 0.0021 | 0.0712 | 0.0051 | 0.0088 | 0.003 | 0.0001 | 0.0074 |
Golgi | 0.0108 | 0.0009 | 0.0018 | 0.0228 | 0.0206 | 0.004 | 0.0093 | 0 | 0.0071 | 0.0073 | 0.0106 | 0.001 | 0.0136 | 0.0001 | 0.0098 | 0.0026 | 0 | 0.0114 |
Lipid Particles | 0.0202 | 0.001 | 0.0025 | 0.0268 | 0.0244 | 0.0107 | 0.0214 | 0.0001 | 0.0039 | 0.0067 | 0.0092 | 0.0013 | 0.0273 | 0.0002 | 0.0066 | 0.0129 | 0 | 0.0062 |
Mitochondria | 0.0152 | 0.0057 | 0.005 | 0.0049 | 0.0043 | 0.0056 | 0.008 | 0.0003 | 0.0144 | 0.0129 | 0.0534 | 0.0063 | 0.0267 | 0.0016 | 0.0119 | 0.0464 | 0.0004 | 0.0232 |
None | 0.0184 | 0.0012 | 0.0014 | 0.0003 | 0.0063 | 0.0005 | 0.0148 | 0.0033 | 0.0037 | 0.0015 | 0.0249 | 0.0007 | 0.0326 | 0.0076 | 0.0134 | 0.0019 | 0.0005 | 0.0004 |
Nuclear Periphery | 0.03 | 0.0123 | 0.0128 | 0.0215 | 0.0453 | 0.0102 | 0.0445 | 0.0222 | 0.0231 | 0.0411 | 0.0971 | 0.0081 | 0.0321 | 0.0106 | 0.0084 | 0.0033 | 0.0057 | 0.0043 |
Nucleolus | 0.0268 | 0.028 | 0.0529 | 0.0173 | 0.0273 | 0.068 | 0.0295 | 0.0428 | 0.0423 | 0.0508 | 0.0278 | 0.075 | 0.0301 | 0.0496 | 0.06 | 0.0337 | 0.0139 | 0.0526 |
Nucleus | 0.6865 | 0.9295 | 0.8492 | 0.8753 | 0.672 | 0.8702 | 0.6868 | 0.9045 | 0.8291 | 0.7958 | 0.5657 | 0.8751 | 0.5854 | 0.8929 | 0.8039 | 0.8292 | 0.9717 | 0.8479 |
Peroxisomes | 0.0188 | 0.0015 | 0.0018 | 0.0015 | 0.0108 | 0.0027 | 0.0052 | 0 | 0.0025 | 0.0026 | 0.0092 | 0.0012 | 0.0067 | 0.0001 | 0.0093 | 0.0067 | 0 | 0.002 |
Punctate Nuclear | 0.0534 | 0.0115 | 0.0225 | 0.0027 | 0.0188 | 0.0046 | 0.0452 | 0.0128 | 0.0134 | 0.0124 | 0.0959 | 0.0191 | 0.0174 | 0.0056 | 0.034 | 0.0418 | 0.0036 | 0.0095 |
Vacuole | 0.0091 | 0.0005 | 0.0026 | 0.0005 | 0.0054 | 0.0012 | 0.0047 | 0.0017 | 0.0037 | 0.0138 | 0.0106 | 0.001 | 0.0099 | 0.0045 | 0.0012 | 0.0008 | 0.0004 | 0.0047 |
Vacuole Periphery | 0.0035 | 0.0003 | 0.001 | 0.0007 | 0.0024 | 0.0008 | 0.0027 | 0.0005 | 0.001 | 0.0022 | 0.0064 | 0.0003 | 0.0039 | 0.0006 | 0.0005 | 0.0005 | 0.0002 | 0.0011 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 9.0021 | 5.7481 | 8.9572 | 9.0986 | 6.2527 | 10.2825 | 7.7008 | 7.2569 | 8.8046 | 12.4777 |
Translational Efficiency | 1.1843 | 1.8132 | 0.9949 | 1.0499 | 1.6961 | 1.0959 | 0.7987 | 0.9668 | 0.8122 | 0.7547 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
185 | 146 | 192 | 218 | 2126 | 2080 | 1672 | 310 | 2311 | 2226 | 1864 | 528 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 662.40 | 825.38 | 1072.84 | 961.86 | 737.76 | 738.96 | 910.27 | 882.99 | 731.73 | 744.63 | 927.02 | 915.55 |
Standard Deviation | 75.91 | 106.89 | 130.60 | 115.15 | 84.42 | 96.53 | 116.36 | 129.90 | 86.23 | 99.57 | 127.84 | 129.96 |
Intensity Change Log 2 | — | 0.317356 | 0.695660 | 0.538124 | — | 0.002345 | 0.303143 | 0.259246 | — | 0.159962 | 0.502173 | 0.397912 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000064 | 0.000355 | 0.001894 | 0.000925 | 0.000038 | 0.000229 | 0.000288 | 0.003509 | 0.000040 | 0.000237 | 0.000453 | 0.002442 |
Bud Neck | 0.001416 | 0.002863 | 0.007382 | 0.017972 | 0.002915 | 0.009834 | 0.010246 | 0.010262 | 0.002795 | 0.009377 | 0.009951 | 0.013445 |
Bud Site | 0.000573 | 0.000523 | 0.001829 | 0.032810 | 0.000492 | 0.002610 | 0.001703 | 0.022481 | 0.000499 | 0.002473 | 0.001716 | 0.026746 |
Cell Periphery | 0.000201 | 0.000066 | 0.000128 | 0.000166 | 0.000106 | 0.000109 | 0.000115 | 0.000197 | 0.000114 | 0.000106 | 0.000116 | 0.000184 |
Cytoplasm | 0.129104* | 0.033375 | 0.007560 | 0.064337 | 0.085796 | 0.065381 | 0.033760 | 0.069087 | 0.089263 | 0.063282 | 0.031061 | 0.067126 |
Cytoplasmic Foci | 0.023625 | 0.004360 | 0.000830 | 0.004782 | 0.007767 | 0.008580 | 0.000852 | 0.006633 | 0.009036 | 0.008303 | 0.000849 | 0.005869 |
Eisosomes | 0.000051 | 0.000044 | 0.000072 | 0.000040 | 0.000018 | 0.000022 | 0.000018 | 0.000041 | 0.000021 | 0.000024 | 0.000023 | 0.000040 |
Endoplasmic Reticulum | 0.001177 | 0.006056 | 0.020028 | 0.010171 | 0.001717 | 0.003682 | 0.004643 | 0.004577 | 0.001673 | 0.003838 | 0.006228 | 0.006887 |
Endosome | 0.000913 | 0.000743 | 0.001044 | 0.017514 | 0.000969 | 0.002648 | 0.000737 | 0.011091 | 0.000964 | 0.002523 | 0.000769 | 0.013743 |
Golgi | 0.000173 | 0.000092 | 0.000116 | 0.004143 | 0.000231 | 0.000993 | 0.000135 | 0.010982 | 0.000226 | 0.000934 | 0.000133 | 0.008158 |
Lipid Particles | 0.003250 | 0.002368 | 0.001329 | 0.000698 | 0.002200 | 0.002162 | 0.000344 | 0.001595 | 0.002284 | 0.002175 | 0.000445 | 0.001224 |
Mitochondria | 0.000835 | 0.000852 | 0.001257 | 0.006521 | 0.001645 | 0.008152 | 0.001718 | 0.008481 | 0.001580 | 0.007674 | 0.001671 | 0.007672 |
Mitotic Spindle | 0.000151 | 0.000123 | 0.000163 | 0.063308 | 0.000807 | 0.009247 | 0.008721 | 0.039853 | 0.000754 | 0.008648 | 0.007840 | 0.049537 |
None | 0.018518 | 0.004074 | 0.001377 | 0.011524 | 0.004806 | 0.004641 | 0.006405 | 0.016445 | 0.005904 | 0.004603 | 0.005887 | 0.014413 |
Nuclear Periphery | 0.001620 | 0.002130 | 0.001744 | 0.003582 | 0.003928 | 0.005053 | 0.002647 | 0.001495 | 0.003743 | 0.004862 | 0.002554 | 0.002357 |
Nuclear Periphery Foci | 0.000391 | 0.000906 | 0.000699 | 0.013895 | 0.000575 | 0.001014 | 0.000386 | 0.001081 | 0.000560 | 0.001007 | 0.000418 | 0.006372 |
Nucleolus | 0.007239 | 0.009313 | 0.003319 | 0.003429 | 0.005225 | 0.006700 | 0.002761 | 0.002402 | 0.005386 | 0.006871 | 0.002818 | 0.002826 |
Nucleus | 0.805733* | 0.924488* | 0.930882* | 0.707251* | 0.877909* | 0.855518* | 0.919401* | 0.755998* | 0.872131* | 0.860041* | 0.920584* | 0.735871* |
Peroxisomes | 0.001802 | 0.000984 | 0.000419 | 0.000560 | 0.000620 | 0.002308 | 0.000193 | 0.002020 | 0.000715 | 0.002221 | 0.000216 | 0.001417 |
Vacuole | 0.002832 | 0.006049 | 0.017668 | 0.032933 | 0.002044 | 0.009654 | 0.004604 | 0.023800 | 0.002107 | 0.009417 | 0.005950 | 0.027571 |
Vacuole Periphery | 0.000331 | 0.000234 | 0.000261 | 0.003440 | 0.000194 | 0.001463 | 0.000323 | 0.007972 | 0.000205 | 0.001382 | 0.000316 | 0.006101 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -4.18 | -13.58 | -10.33 | -6.96 | 2.60 | -4.60 | -15.84 | -2.49 | -2.23 | -2.01 | -5.11 | -19.00 | -3.80 | -3.35 | -2.63 |
Bud Neck | -2.57 | -9.50 | -8.57 | -7.47 | -5.27 | -7.08 | -10.24 | -6.86 | -3.07 | -1.25 | -7.25 | -10.91 | -10.25 | -5.32 | -3.11 |
Bud Site | -0.52 | -5.81 | -3.33 | -3.30 | -2.90 | -5.41 | -4.68 | -3.21 | -2.71 | -2.83 | -5.44 | -5.26 | -4.57 | -3.91 | -4.02 |
Cell Periphery | 3.79 | 1.34 | 1.53 | -3.72 | 0.44 | -0.08 | 0.15 | -1.35 | -1.28 | -1.36 | 0.58 | 0.31 | -1.47 | -1.94 | -1.62 |
Cytoplasm | 5.32 | 7.64 | 5.34 | -0.41 | -4.34 | 4.38 | 13.38 | 4.89 | 2.19 | -2.87 | 5.74 | 15.31 | 7.68 | 3.44 | -3.23 |
Cytoplasmic Foci | 4.40 | 5.74 | 5.56 | 2.21 | -0.78 | -0.90 | 8.98 | 4.63 | 5.42 | -2.01 | 0.48 | 10.47 | 7.36 | 7.01 | -2.18 |
Eisosomes | 0.38 | -3.47 | -3.36 | -3.65 | -0.29 | -2.93 | -3.04 | -6.34 | -5.35 | -5.58 | -2.21 | -4.97 | -9.56 | -8.66 | -7.88 |
Endoplasmic Reticulum | -5.20 | -14.24 | -10.29 | -5.56 | 3.06 | -7.77 | -16.05 | -8.92 | -6.28 | -3.42 | -8.90 | -19.59 | -13.12 | -9.97 | -5.02 |
Endosome | -0.02 | -1.18 | -1.84 | -1.85 | -1.65 | -3.91 | 0.73 | -2.82 | -1.50 | -2.96 | -3.91 | 0.46 | -3.17 | -1.87 | -3.26 |
Golgi | 0.62 | 0.42 | -1.71 | -1.85 | -1.81 | -2.74 | 0.98 | -1.86 | -1.48 | -1.92 | -2.73 | 1.01 | -2.12 | -1.51 | -2.22 |
Lipid Particles | 0.49 | 2.42 | 3.12 | 2.19 | 1.23 | -0.25 | 6.98 | 2.77 | 2.85 | -2.79 | -0.12 | 7.23 | 3.92 | 3.72 | -3.60 |
Mitochondria | -0.60 | -2.29 | -2.35 | -2.22 | -1.88 | -6.09 | -0.87 | -1.71 | 1.59 | -1.53 | -6.12 | -0.95 | -2.17 | 2.35 | -1.91 |
Mitotic Spindle | -0.94 | -1.06 | -4.04 | -3.96 | -4.03 | -5.27 | -5.37 | -4.27 | -3.05 | -2.69 | -5.31 | -5.36 | -5.83 | -4.39 | -4.04 |
None | 2.32 | 2.98 | 1.76 | -1.97 | -5.47 | 0.32 | -1.02 | -3.06 | -3.21 | -2.52 | 1.64 | 0.69 | -2.66 | -3.88 | -3.14 |
Nuclear Periphery | -1.20 | -0.93 | -3.97 | -2.61 | -4.41 | -1.95 | 0.97 | 1.45 | 2.82 | 0.28 | -2.12 | 0.95 | -0.30 | 1.85 | -1.14 |
Nuclear Periphery Foci | -2.00 | -2.42 | -1.68 | -1.34 | -1.46 | -1.85 | -0.33 | -1.83 | 0.29 | -1.10 | -2.02 | -0.49 | -1.88 | -1.21 | -1.70 |
Nucleolus | -1.51 | 2.22 | 0.86 | 2.40 | -2.48 | -1.94 | 2.30 | -0.57 | 0.18 | -1.34 | -2.23 | 2.79 | -0.39 | 0.89 | -1.77 |
Nucleus | -5.04 | -5.39 | 2.25 | 7.61 | 8.19 | 3.56 | -5.08 | 5.61 | 4.07 | 7.48 | 2.16 | -6.46 | 7.96 | 6.76 | 10.91 |
Peroxisomes | 0.78 | 1.61 | 1.71 | 2.11 | 0.98 | -4.18 | 3.03 | -0.38 | 3.19 | -2.06 | -3.95 | 3.40 | 0.62 | 4.25 | -2.21 |
Vacuole | 0.04 | -3.29 | -6.72 | -7.33 | -5.13 | -7.58 | -7.74 | -6.71 | -5.42 | -5.79 | -6.91 | -8.39 | -10.18 | -8.84 | -8.87 |
Vacuole Periphery | 0.34 | 0.25 | -1.66 | -2.07 | -2.03 | -3.70 | -0.80 | -1.91 | -1.01 | -1.85 | -3.67 | -0.76 | -2.28 | -0.86 | -2.18 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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