Standard name | |
---|---|
Human Ortholog | |
Description | Mitochondrial glycerol-3-phosphate dehydrogenase; expression is repressed by both glucose and cAMP and derepressed by non-fermentable carbon sources in a Snf1p, Rsf1p, Hap2/3/4/5 complex dependent manner |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 2 | 4 | 4 | 1 | 0 | 0 | – | 0 | 0 | 1 | 2 | 4 | 3 | 5 | 11 | 3 | 6 | 1 | 19 | 2 | 3 | 1 | 6 |
Bud | 11 | 10 | 1 | 4 | 9 | 10 | 8 | – | 7 | 8 | 7 | 12 | 0 | 3 | 0 | 16 | 5 | 10 | 3 | 3 | 0 | 1 | 1 | 1 |
Bud Neck | 2 | 0 | 2 | 1 | 0 | 0 | 0 | – | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 3 | 0 | 1 | 2 | 1 |
Bud Site | 2 | 1 | 0 | 0 | 0 | 0 | 4 | – | 3 | 2 | 7 | 5 | 0 | 0 | 0 | 1 | 2 | 8 | – | – | – | – | – | – |
Cell Periphery | 5 | 17 | 3 | 4 | 5 | 6 | 3 | – | 1 | 3 | 4 | 1 | 1 | 3 | 7 | 9 | 14 | 12 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 15 | 17 | 27 | 17 | 15 | 11 | 30 | – | 10 | 11 | 20 | 5 | 2 | 29 | 55 | 19 | 18 | 15 | 1 | 3 | 2 | 2 | 4 | 1 |
Endoplasmic Reticulum | 4 | 10 | 6 | 2 | 6 | 7 | 3 | – | 4 | 1 | 3 | 6 | 6 | 4 | 10 | 6 | 5 | 3 | 2 | 4 | 0 | 1 | 1 | 2 |
Endosome | 18 | 44 | 15 | 10 | 30 | 15 | 15 | – | 1 | 10 | 0 | 0 | 7 | 15 | 18 | 20 | 26 | 22 | 31 | 40 | 24 | 13 | 10 | 18 |
Golgi | 4 | 22 | 3 | 7 | 16 | 4 | 7 | – | 0 | 2 | 0 | 0 | 17 | 16 | 23 | 56 | 73 | 59 | 16 | 46 | 3 | 3 | 9 | 8 |
Mitochondria | 330 | 322 | 40 | 117 | 156 | 234 | 271 | – | 229 | 225 | 296 | 276 | 23 | 52 | 23 | 20 | 26 | 22 | 274 | 352 | 169 | 122 | 156 | 184 |
Nucleus | 3 | 0 | 2 | 2 | 2 | 8 | 10 | – | 5 | 10 | 3 | 8 | 0 | 1 | 2 | 4 | 6 | 2 | 1 | 1 | 0 | 0 | 1 | 2 |
Nuclear Periphery | 3 | 6 | 1 | 1 | 2 | 5 | 7 | – | 3 | 4 | 12 | 8 | 1 | 0 | 0 | 0 | 4 | 0 | 3 | 3 | 0 | 0 | 0 | 3 |
Nucleolus | 3 | 1 | 0 | 0 | 3 | 4 | 5 | – | 8 | 2 | 4 | 4 | 0 | 1 | 0 | 14 | 8 | 12 | 1 | 0 | 0 | 0 | 0 | 1 |
Peroxisomes | 4 | 28 | 5 | 14 | 3 | 6 | 4 | – | 0 | 1 | 2 | 1 | 1 | 11 | 3 | 40 | 36 | 55 | 10 | 16 | 11 | 8 | 3 | 9 |
SpindlePole | 9 | 11 | 15 | 12 | 7 | 7 | 14 | – | 8 | 13 | 8 | 9 | 2 | 4 | 3 | 48 | 42 | 51 | 4 | 3 | 1 | 2 | 3 | 4 |
Vac/Vac Membrane | 46 | 84 | 25 | 9 | 43 | 29 | 28 | – | 12 | 19 | 22 | 13 | 12 | 19 | 19 | 36 | 31 | 52 | 25 | 24 | 10 | 8 | 15 | 15 |
Unique Cell Count | 366 | 438 | 129 | 160 | 243 | 289 | 334 | 252 | 260 | 324 | 299 | 58 | 126 | 136 | 235 | 241 | 246 | 385 | 530 | 233 | 177 | 218 | 266 | |
Labelled Cell Count | 459 | 575 | 149 | 204 | 298 | 346 | 409 | 291 | 312 | 389 | 351 | 76 | 161 | 168 | 300 | 299 | 329 | 385 | 530 | 233 | 177 | 218 | 266 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 4.9 | 6.0 | 5.9 | 6.2 | 6.8 | 5.9 | 6.1 | – | 5.1 | 5.0 | 4.5 | 4.6 | 6.9 | 6.9 | 6.5 | 10.8 | 11.4 | 11.5 | 5.2 | 5.9 | 6.0 |
Std Deviation (1e-4) | 0.7 | 1.1 | 1.1 | 1.7 | 1.4 | 1.4 | 1.7 | – | 1.5 | 1.2 | 1.4 | 0.9 | 0.9 | 1.2 | 1.1 | 2.6 | 3.0 | 3.0 | 1.1 | 1.3 | 1.4 |
Intensity Change (Log2) | – | – | – | 0.09 | 0.22 | 0.0 | 0.05 | – | -0.2 | -0.24 | -0.37 | -0.34 | 0.23 | 0.24 | 0.15 | 0.88 | 0.96 | 0.97 | -0.16 | 0.02 | 0.04 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0.7 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2.6 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1.5 | 1.2 |
Cytoplasm | -2.4 | -4.3 | -5.6 | -3.5 | – | -5.3 | -5.2 | -4.6 | -7.0 | -3.1 | 0.4 | 3.4 | -3.5 | -3.8 | -4.3 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0.9 | 0 | 0 | 0 |
Endosome | -1.6 | 0.2 | -2.4 | -2.8 | – | -5.2 | -2.9 | 0 | 0 | 0.1 | 0.1 | 0.4 | -1.0 | -0.2 | -0.8 |
Golgi | 0 | 1.8 | 0 | 0 | – | 0 | 0 | 0 | 0 | 5.5 | 3.2 | 4.0 | 5.3 | 6.3 | 5.4 |
Mitochondria | 7.1 | 6.1 | 9.9 | 10.3 | – | 12.1 | 11.1 | 0 | 0 | 1.2 | 1.7 | -2.7 | -5.5 | -4.8 | -5.5 |
Nucleus | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 1.7 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 1.6 | 0 | 3.6 | 3.2 | 4.6 |
SpindlePole | -1.2 | -3.4 | -3.9 | -3.0 | – | -3.3 | -2.4 | 0 | 0 | -1.8 | -2.6 | -3.0 | 2.1 | 1.5 | 2.2 |
Vacuole | -3.6 | -0.4 | -2.6 | -3.3 | – | -4.6 | -3.5 | -4.0 | -5.0 | 0.2 | -0.9 | -1.2 | -1.0 | -1.7 | 0.4 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 1.7921 | 1.7791 | 1.5301 | 2.0488 | 0.8738 | 1.6873 | 3.1037 | 3.5995 | 3.3215 | 3.2982 | 3.0584 | 3.2388 | 3.019 | 3.6212 | 3.0739 | 3.9151 | 3.1358 | 3.3118 |
Actin | 0.0406 | 0.0253 | 0.009 | 0.0037 | 0.0044 | 0.0117 | 0.0109 | 0.0129 | 0.019 | 0.0026 | 0.0045 | 0.0085 | 0.0184 | 0.0059 | 0.0031 | 0.0369 | 0.0031 | 0.0018 |
Bud | 0.001 | 0.0025 | 0.0011 | 0.0014 | 0.0093 | 0.0014 | 0.0017 | 0.0016 | 0.0024 | 0.0022 | 0.0003 | 0.0005 | 0.0006 | 0.0017 | 0.001 | 0.0006 | 0.0004 | 0.0001 |
Bud Neck | 0.0009 | 0.0016 | 0.0006 | 0.0008 | 0.0007 | 0.0007 | 0.0007 | 0.0018 | 0.0015 | 0.0007 | 0.0003 | 0.0006 | 0.0007 | 0.0008 | 0.0003 | 0.0002 | 0 | 0.0001 |
Bud Periphery | 0.0019 | 0.0025 | 0.0017 | 0.009 | 0.0245 | 0.0066 | 0.0015 | 0.0027 | 0.0067 | 0.0084 | 0.0006 | 0.0013 | 0.001 | 0.0026 | 0.0017 | 0.0008 | 0.0004 | 0.0002 |
Bud Site | 0.0061 | 0.0218 | 0.0024 | 0.0012 | 0.003 | 0.0003 | 0.0035 | 0.0327 | 0.0042 | 0.0009 | 0.0005 | 0.0023 | 0.0021 | 0.0073 | 0.0016 | 0.0007 | 0.0008 | 0.0001 |
Cell Periphery | 0.0009 | 0.0009 | 0.0003 | 0.0132 | 0.0013 | 0.0003 | 0.0007 | 0.0034 | 0.0007 | 0.0004 | 0.0001 | 0.0004 | 0.0005 | 0.0005 | 0.0003 | 0.0002 | 0.0001 | 0.0001 |
Cytoplasm | 0.0019 | 0.005 | 0.0036 | 0.003 | 0.0059 | 0.0007 | 0.0032 | 0.0025 | 0.002 | 0.0006 | 0.0015 | 0.0006 | 0.0028 | 0.0015 | 0.0007 | 0.0017 | 0.0001 | 0.0006 |
Cytoplasmic Foci | 0.0267 | 0.0175 | 0.0304 | 0.0147 | 0.0261 | 0.0029 | 0.0295 | 0.0179 | 0.0165 | 0.0108 | 0.0122 | 0.0084 | 0.018 | 0.0244 | 0.012 | 0.0166 | 0.0105 | 0.0078 |
Eisosomes | 0.0007 | 0.0004 | 0.0003 | 0.0002 | 0.0002 | 0.0003 | 0.0004 | 0.0007 | 0.0004 | 0.0002 | 0.0001 | 0.0003 | 0.0004 | 0.0003 | 0.0002 | 0.0002 | 0.0001 | 0.0001 |
Endoplasmic Reticulum | 0.0026 | 0.0028 | 0.0016 | 0.0008 | 0.0011 | 0.0008 | 0.002 | 0.002 | 0.0022 | 0.0006 | 0.0009 | 0.0008 | 0.0004 | 0.0002 | 0.0002 | 0.0002 | 0 | 0.0002 |
Endosome | 0.054 | 0.0505 | 0.0501 | 0.0962 | 0.099 | 0.0198 | 0.0597 | 0.0529 | 0.0258 | 0.0415 | 0.0514 | 0.0353 | 0.0473 | 0.0521 | 0.0318 | 0.0391 | 0.0023 | 0.0191 |
Golgi | 0.0287 | 0.031 | 0.0767 | 0.1041 | 0.0725 | 0.0358 | 0.0471 | 0.0545 | 0.0272 | 0.0543 | 0.0759 | 0.0467 | 0.0404 | 0.0484 | 0.0511 | 0.076 | 0.0079 | 0.0481 |
Lipid Particles | 0.0366 | 0.0101 | 0.0136 | 0.0103 | 0.0041 | 0.0011 | 0.0438 | 0.0075 | 0.0063 | 0.0177 | 0.0127 | 0.0088 | 0.0328 | 0.0183 | 0.004 | 0.002 | 0.0022 | 0.0024 |
Mitochondria | 0.7117 | 0.7642 | 0.7469 | 0.7151 | 0.7078 | 0.9123 | 0.7309 | 0.765 | 0.8505 | 0.8388 | 0.7784 | 0.854 | 0.7746 | 0.7824 | 0.8726 | 0.814 | 0.9184 | 0.9123 |
None | 0.0031 | 0.0029 | 0.0045 | 0.0003 | 0.0005 | 0.0001 | 0.0048 | 0.0005 | 0.0067 | 0.0003 | 0.0016 | 0.0004 | 0.0092 | 0.0002 | 0.0001 | 0.0002 | 0.0001 | 0.0001 |
Nuclear Periphery | 0.0058 | 0.0007 | 0.0006 | 0.0002 | 0.0005 | 0.0002 | 0.009 | 0.0017 | 0.0042 | 0.0007 | 0.0138 | 0.0007 | 0.0012 | 0.0001 | 0.0001 | 0.0001 | 0 | 0 |
Nucleolus | 0.0006 | 0.0006 | 0.0003 | 0.0002 | 0.0002 | 0 | 0.0009 | 0.0008 | 0.0002 | 0.0002 | 0.0007 | 0.0002 | 0.0021 | 0.0009 | 0.0001 | 0 | 0.0001 | 0 |
Nucleus | 0.0009 | 0.0005 | 0.0003 | 0.0003 | 0.0007 | 0.0001 | 0.0019 | 0.0005 | 0.0004 | 0.0005 | 0.0038 | 0.0004 | 0.0023 | 0.0003 | 0.0001 | 0.0001 | 0 | 0 |
Peroxisomes | 0.0381 | 0.0352 | 0.0381 | 0.0093 | 0.0189 | 0.0022 | 0.0333 | 0.0213 | 0.0152 | 0.0052 | 0.0238 | 0.0237 | 0.0263 | 0.0416 | 0.0148 | 0.0057 | 0.0526 | 0.0047 |
Punctate Nuclear | 0.0058 | 0.0001 | 0.0003 | 0.0001 | 0.0001 | 0 | 0.0066 | 0.0002 | 0.0004 | 0.0001 | 0.0123 | 0.0001 | 0.0008 | 0.0001 | 0.0001 | 0 | 0 | 0 |
Vacuole | 0.013 | 0.0186 | 0.0099 | 0.0084 | 0.0115 | 0.0011 | 0.0036 | 0.012 | 0.0037 | 0.0071 | 0.0015 | 0.0023 | 0.0089 | 0.0072 | 0.002 | 0.0019 | 0.0003 | 0.0007 |
Vacuole Periphery | 0.0183 | 0.0053 | 0.0077 | 0.0072 | 0.0076 | 0.0013 | 0.0044 | 0.0051 | 0.004 | 0.0064 | 0.003 | 0.0036 | 0.0091 | 0.0031 | 0.002 | 0.003 | 0.0006 | 0.0015 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 22.0681 | 18.7159 | 22.1457 | 23.5627 | 11.3715 | 29.9734 | 33.1163 | 36.9357 | 40.0017 | 24.0065 |
Translational Efficiency | 0.692 | 0.5561 | 0.6354 | 0.8971 | 0.6983 | 0.8155 | 0.65 | 0.7028 | 0.7307 | 0.5342 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
180 | 1228 | 1592 | 1569 | 2421 | 1807 | 170 | 187 | 2601 | 3035 | 1762 | 1756 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 618.50 | 760.85 | 1077.94 | 900.02 | 769.04 | 774.74 | 1114.52 | 1007.13 | 758.62 | 769.12 | 1081.47 | 911.43 |
Standard Deviation | 69.31 | 106.56 | 172.98 | 125.84 | 97.54 | 118.44 | 138.16 | 158.67 | 103.19 | 113.98 | 170.28 | 133.87 |
Intensity Change Log 2 | — | 0.298838 | 0.801431 | 0.541183 | — | 0.010654 | 0.535292 | 0.389119 | — | 0.146264 | 0.660021 | 0.458889 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000805 | 0.003013 | 0.018328 | 0.024887 | 0.000766 | 0.000696 | 0.007250 | 0.048602 | 0.000769 | 0.001634 | 0.017259 | 0.027412 |
Bud Neck | 0.031905 | 0.007549 | 0.002572 | 0.011386 | 0.002014 | 0.001726 | 0.016030 | 0.010801 | 0.004083 | 0.004082 | 0.003870 | 0.011324 |
Bud Site | 0.013365 | 0.032481 | 0.016468 | 0.142015* | 0.007590 | 0.006894 | 0.078683 | 0.183697* | 0.007990 | 0.017247 | 0.022471 | 0.146454* |
Cell Periphery | 0.001112 | 0.001151 | 0.000327 | 0.000757 | 0.000201 | 0.000223 | 0.000768 | 0.000633 | 0.000264 | 0.000599 | 0.000369 | 0.000744 |
Cytoplasm | 0.026666 | 0.008812 | 0.003978 | 0.063096 | 0.002450 | 0.002903 | 0.007239 | 0.033508 | 0.004126 | 0.005293 | 0.004293 | 0.059945 |
Cytoplasmic Foci | 0.160374 | 0.047244 | 0.086949 | 0.101844 | 0.044408 | 0.033893 | 0.103241 | 0.105961 | 0.052433 | 0.039295 | 0.088521 | 0.102282 |
Eisosomes | 0.001211 | 0.000105 | 0.000132 | 0.000164 | 0.000060 | 0.000053 | 0.000137 | 0.000193 | 0.000139 | 0.000074 | 0.000133 | 0.000167 |
Endoplasmic Reticulum | 0.000259 | 0.000462 | 0.001512 | 0.001144 | 0.000356 | 0.000332 | 0.007412 | 0.001516 | 0.000349 | 0.000385 | 0.002081 | 0.001184 |
Endosome | 0.053476 | 0.103140 | 0.079709 | 0.065033 | 0.082483 | 0.077874 | 0.108757 | 0.076292 | 0.080476 | 0.088097 | 0.082512 | 0.066232 |
Golgi | 0.120629* | 0.119735* | 0.176958* | 0.114809* | 0.137931* | 0.124532* | 0.111064* | 0.137330* | 0.136734* | 0.122591* | 0.170600* | 0.117207* |
Lipid Particles | 0.018567 | 0.001691 | 0.003835 | 0.000912 | 0.002426 | 0.001350 | 0.005438 | 0.001038 | 0.003543 | 0.001488 | 0.003990 | 0.000925 |
Mitochondria | 0.482493* | 0.574184* | 0.447243* | 0.232123* | 0.650316* | 0.672002* | 0.230474* | 0.155066* | 0.638702* | 0.632424* | 0.426329* | 0.223917* |
Mitotic Spindle | 0.002403 | 0.018733 | 0.062015 | 0.085921 | 0.011035 | 0.013111 | 0.142715* | 0.086258 | 0.010438 | 0.015386 | 0.069801 | 0.085957 |
None | 0.001179 | 0.000641 | 0.000402 | 0.000247 | 0.000193 | 0.000296 | 0.000225 | 0.000210 | 0.000261 | 0.000436 | 0.000385 | 0.000243 |
Nuclear Periphery | 0.000065 | 0.000064 | 0.000710 | 0.000509 | 0.000230 | 0.000260 | 0.001704 | 0.000065 | 0.000218 | 0.000180 | 0.000805 | 0.000462 |
Nuclear Periphery Foci | 0.000122 | 0.000200 | 0.001006 | 0.000380 | 0.000288 | 0.000275 | 0.003699 | 0.000241 | 0.000277 | 0.000245 | 0.001266 | 0.000365 |
Nucleolus | 0.002231 | 0.000602 | 0.000173 | 0.000254 | 0.000087 | 0.000267 | 0.001032 | 0.000056 | 0.000235 | 0.000403 | 0.000256 | 0.000233 |
Nucleus | 0.004279 | 0.000838 | 0.000182 | 0.001591 | 0.000195 | 0.000425 | 0.002627 | 0.000366 | 0.000477 | 0.000592 | 0.000418 | 0.001461 |
Peroxisomes | 0.062288 | 0.040490 | 0.032787 | 0.071303 | 0.027619 | 0.015317 | 0.093693 | 0.121438* | 0.030018 | 0.025502 | 0.038663 | 0.076642 |
Vacuole | 0.006316 | 0.021522 | 0.033537 | 0.064336 | 0.012551 | 0.020930 | 0.055914 | 0.027866 | 0.012120 | 0.021170 | 0.035696 | 0.060452 |
Vacuole Periphery | 0.010254 | 0.017342 | 0.031177 | 0.017287 | 0.016800 | 0.026641 | 0.021898 | 0.008864 | 0.016347 | 0.022878 | 0.030282 | 0.016390 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -3.84 | -9.75 | -11.29 | -9.95 | -2.17 | 0.42 | -5.50 | -5.73 | -5.74 | -4.89 | -3.46 | -10.18 | -12.67 | -12.19 | -3.67 |
Bud Neck | 2.92 | 3.54 | 2.39 | -3.52 | -10.41 | 0.83 | -4.22 | -3.91 | -4.03 | 1.29 | -0.06 | 0.23 | -7.54 | -8.55 | -8.78 |
Bud Site | -4.49 | -0.98 | -21.72 | -19.47 | -24.80* | 0.70 | -6.68 | -10.70 | -10.74 | -5.29 | -6.66 | -8.66 | -28.89 | -26.44 | -24.83 |
Cell Periphery | -0.09 | 2.19 | 0.91 | 0.88 | -2.00 | -1.03 | -2.62 | -6.27 | -5.85 | 0.59 | -1.91 | -2.60 | -2.51 | -0.49 | -1.93 |
Cytoplasm | 2.83 | 3.68 | -5.99 | -17.33 | -20.19 | -0.80 | -4.50 | -7.74 | -7.59 | -6.35 | -1.50 | -0.39 | -20.80 | -20.25 | -20.88 |
Cytoplasmic Foci | 8.36 | 5.45 | 4.38 | -13.94 | -3.32 | 4.94 | -6.53 | -6.89 | -8.07 | -0.23 | 6.05 | -10.40 | -14.08 | -19.12 | -3.26 |
Eisosomes | 1.23 | 1.20 | 1.17 | -5.34 | -2.95 | 1.99 | -6.68 | -6.90 | -7.23 | -2.42 | 1.04 | 0.09 | -0.42 | -11.52 | -3.48 |
Endoplasmic Reticulum | -2.36 | -5.16 | -7.10 | -5.52 | 1.34 | 0.23 | -1.81 | -2.41 | -2.46 | 1.45 | -0.39 | -3.95 | -6.28 | -6.65 | 1.92 |
Endosome | -6.50 | -3.88 | -1.62 | 7.87 | 4.41 | 1.15 | -2.76 | 0.87 | 0.28 | 2.82 | -2.15 | -0.57 | 4.39 | 6.72 | 5.05 |
Golgi | -0.02 | -4.11 | 0.65 | 1.42 | 9.70 | 2.41 | 2.32 | 0.22 | -0.73 | -1.40 | 2.97 | -5.46 | 4.40 | 1.84 | 8.90 |
Lipid Particles | 4.42 | 3.90 | 4.61 | 1.40 | 5.70 | 1.80 | -1.83 | 1.58 | 0.02 | 2.49 | 3.51 | -0.53 | 4.28 | 1.20 | 6.22 |
Mitochondria | -3.44 | 1.07 | 9.22 | 28.35* | 18.96* | -2.13 | 17.68 | 25.56 | 26.29 | 2.57 | 0.68 | 19.44 | 44.26* | 45.35* | 18.87* |
Mitotic Spindle | -8.82 | -16.87 | -18.68 | -14.37 | -4.61 | -1.33 | -7.81 | -6.99 | -6.76 | 2.85 | -3.73 | -16.25 | -18.11 | -16.74 | -3.31 |
None | 1.37 | 2.19 | 2.55 | 1.43 | 7.35 | -2.71 | -1.21 | -0.37 | 1.88 | 0.45 | -1.39 | -3.04 | 0.86 | 1.71 | 7.31 |
Nuclear Periphery | 0.22 | -8.42 | -2.28 | -2.29 | 0.66 | -0.19 | -1.56 | 1.68 | 1.49 | 1.74 | 0.33 | -4.10 | -1.36 | -1.58 | 1.33 |
Nuclear Periphery Foci | -0.11 | -6.28 | -2.10 | -2.28 | 4.45 | 0.09 | -1.91 | -0.01 | -0.12 | 1.91 | 0.30 | -4.37 | -0.91 | -1.67 | 4.14 |
Nucleolus | 1.71 | 2.03 | 1.95 | 1.20 | -1.82 | -2.61 | -2.74 | 1.34 | 3.05 | 2.82 | -0.63 | 0.47 | 0.38 | 1.16 | -0.18 |
Nucleus | 1.48 | 1.77 | 1.04 | -2.94 | -6.04 | -1.44 | -2.94 | -1.55 | -0.03 | 2.66 | -0.52 | 0.21 | -4.11 | -4.06 | -4.81 |
Peroxisomes | 2.88 | 3.88 | -0.67 | -6.17 | -8.20 | 5.06 | -5.30 | -6.69 | -7.61 | -1.40 | 1.90 | -2.58 | -10.92 | -12.56 | -8.11 |
Vacuole | -5.76 | -9.95 | -16.20 | -12.78 | -9.48 | -5.75 | -5.08 | -2.67 | -1.59 | 2.03 | -7.16 | -11.58 | -16.64 | -13.64 | -8.32 |
Vacuole Periphery | -3.15 | -7.92 | -2.84 | 0.32 | 6.71 | -5.80 | -1.56 | 3.67 | 7.30 | 3.57 | -5.04 | -7.60 | 0.28 | 4.69 | 7.29 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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