Standard name | |
---|---|
Human Ortholog | |
Description | Subunit of a complex that associates with actin filaments; forms a complex with Tda2p that inhibits barbed end F-actin assembly; elevates actin monomer pools to increase endocytotic efficiency and to regulate the distribution of actin between cables and patches; Aim21p/Tda2p forms a larger complex with actin capping proteins Cap1p and Cap2p; involved in mitochondrial migration along actin filaments; recruited to cortical actin patches by SH3 domain-containing proteins Bbc1p and Abp1p |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 191 | 138 | 67 | 80 | 74 | 120 | 107 | 119 | 35 | 77 | 29 | 76 | 143 | 172 | 158 | – | – | – | 240 | 179 | 87 | 137 | 240 | 296 |
Bud | 48 | 33 | 11 | 24 | 22 | 28 | 36 | 37 | 12 | 35 | 16 | 19 | 31 | 34 | 32 | – | – | – | 18 | 10 | 5 | 25 | 45 | 39 |
Bud Neck | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | 7 | 10 | 7 | 21 | 26 | 26 |
Bud Site | 16 | 2 | 1 | 4 | 4 | 6 | 5 | 5 | 0 | 3 | 0 | 4 | 5 | 4 | 0 | – | – | – | – | – | – | – | – | – |
Cell Periphery | 21 | 20 | 9 | 9 | 13 | 23 | 14 | 16 | 1 | 18 | 4 | 5 | 22 | 47 | 42 | – | – | – | 0 | 1 | 1 | 0 | 1 | 2 |
Cytoplasm | 50 | 35 | 22 | 15 | 43 | 23 | 38 | 54 | 13 | 28 | 17 | 15 | 53 | 53 | 93 | – | – | – | 12 | 17 | 5 | 12 | 13 | 31 |
Endoplasmic Reticulum | 30 | 26 | 11 | 12 | 11 | 35 | 54 | 42 | 20 | 25 | 17 | 29 | 24 | 33 | 47 | – | – | – | 2 | 2 | 3 | 1 | 4 | 7 |
Endosome | 1 | 9 | 5 | 6 | 2 | 2 | 2 | 9 | 5 | 8 | 10 | 6 | 6 | 1 | 5 | – | – | – | 7 | 13 | 13 | 10 | 20 | 12 |
Golgi | 13 | 34 | 13 | 10 | 10 | 17 | 30 | 17 | 9 | 13 | 5 | 19 | 24 | 44 | 35 | – | – | – | 5 | 10 | 3 | 2 | 6 | 3 |
Mitochondria | 66 | 10 | 4 | 10 | 4 | 42 | 39 | 45 | 79 | 84 | 92 | 76 | 2 | 3 | 1 | – | – | – | 9 | 6 | 11 | 5 | 10 | 8 |
Nucleus | 1 | 0 | 2 | 1 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 2 | – | – | – | 0 | 0 | 0 | 1 | 1 | 0 |
Nuclear Periphery | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 1 | 0 | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 | 0 | 0 | 1 |
Nucleolus | 0 | 0 | 0 | 1 | 0 | 0 | 2 | 7 | 0 | 2 | 1 | 0 | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 62 | 18 | 10 | 32 | 11 | 23 | 11 | 6 | 4 | 14 | 7 | 5 | 32 | 36 | 27 | – | – | – | 17 | 4 | 7 | 4 | 2 | 10 |
SpindlePole | 4 | 5 | 2 | 9 | 5 | 2 | 4 | 7 | 0 | 3 | 1 | 1 | 9 | 10 | 9 | – | – | – | 1 | 0 | 0 | 2 | 2 | 1 |
Vac/Vac Membrane | 1 | 1 | 1 | 3 | 1 | 2 | 4 | 15 | 7 | 7 | 2 | 3 | 1 | 2 | 1 | – | – | – | 2 | 2 | 1 | 2 | 2 | 5 |
Unique Cell Count | 295 | 213 | 108 | 129 | 138 | 216 | 221 | 274 | 133 | 209 | 139 | 166 | 227 | 282 | 310 | 329 | 263 | 153 | 230 | 382 | 450 | |||
Labelled Cell Count | 507 | 331 | 159 | 216 | 200 | 324 | 347 | 379 | 187 | 317 | 202 | 259 | 352 | 440 | 452 | 329 | 263 | 153 | 230 | 382 | 450 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 7.4 | 9.9 | 8.9 | 9.1 | 9.6 | 8.1 | 8.4 | 8.6 | 7.1 | 7.5 | 7.0 | 7.3 | 10.1 | 10.3 | 9.6 | – | – | – | 9.3 | 9.2 | 9.5 |
Std Deviation (1e-4) | 1.1 | 1.8 | 1.5 | 2.0 | 1.9 | 1.6 | 1.6 | 1.6 | 1.6 | 1.5 | 2.1 | 1.4 | 1.6 | 1.7 | 1.6 | – | – | – | 1.5 | 1.4 | 1.5 |
Intensity Change (Log2) | – | – | – | 0.04 | 0.11 | -0.12 | -0.08 | -0.05 | -0.31 | -0.23 | -0.35 | -0.29 | 0.19 | 0.22 | 0.12 | – | – | – | 0.07 | 0.06 | 0.1 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | -1.3 | -1.1 | -2.3 | -3.3 | -5.6 | -4.3 | -6.6 | -2.6 | 0.2 | -0.2 | -2.0 | – | – | – |
Bud | 1.8 | 1.3 | 0.7 | 1.5 | 0.9 | -0.3 | 1.6 | 0.3 | 0.3 | 0.9 | 0.5 | 0 | – | – | – |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – |
Cell Periphery | -0.4 | 0.3 | 0.7 | -0.7 | -0.9 | 0 | 0.1 | -1.9 | -2.0 | 0.4 | 2.1 | 1.4 | – | – | – |
Cytoplasm | -1.8 | 1.9 | -2.4 | -0.7 | -0.1 | -2.3 | -1.6 | -1.7 | -2.7 | 0.6 | -0.4 | 1.9 | – | – | – |
Endoplasmic Reticulum | -0.2 | -0.6 | 1.5 | 3.0 | 1.3 | 1.1 | 0.5 | 0.5 | 1.7 | 0.1 | 0.4 | 1.3 | – | – | – |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0.8 | 0 | 0 | 0 | 0 | – | – | – |
Golgi | -1.1 | -1.3 | -1.2 | 0.4 | -1.9 | -1.4 | -1.8 | -2.5 | -0.1 | -0.4 | 0.9 | -0.2 | – | – | – |
Mitochondria | 1.3 | 0 | 3.8 | 3.5 | 3.3 | 9.0 | 6.9 | 10.0 | 7.5 | 0 | 0 | 0 | – | – | – |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – |
Peroxisomes | 3.1 | -0.4 | 0.4 | -1.5 | 0 | -2.1 | -0.8 | -1.3 | -2.2 | 1.2 | 1.0 | -0.2 | – | – | – |
SpindlePole | 1.9 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – |
Vacuole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 2.5051 | 3.016 | 2.9681 | 3.122 | 2.2928 | 2.7506 | 2.9717 | 3.6646 | 3.5711 | 3.0899 | 2.7715 | 3.4586 | 3.8796 | 4.2056 | 4.4034 | 4.3832 | 4.1683 | 4.2539 |
Actin | 0.7981 | 0.6189 | 0.7906 | 0.7433 | 0.6287 | 0.7376 | 0.7138 | 0.6417 | 0.7546 | 0.702 | 0.7535 | 0.8601 | 0.8154 | 0.6348 | 0.7946 | 0.811 | 0.735 | 0.858 |
Bud | 0.0082 | 0.0132 | 0.0322 | 0.024 | 0.0145 | 0.0015 | 0.0065 | 0.0073 | 0.0366 | 0.0209 | 0.0094 | 0.0034 | 0.0018 | 0.0161 | 0.0227 | 0.0144 | 0.0114 | 0.0009 |
Bud Neck | 0.0065 | 0.0044 | 0.0138 | 0.0035 | 0.0015 | 0.1035 | 0.0189 | 0.0083 | 0.0128 | 0.018 | 0.0042 | 0.0612 | 0.0048 | 0.004 | 0.0165 | 0.0123 | 0.0158 | 0.0637 |
Bud Periphery | 0.0078 | 0.012 | 0.056 | 0.0564 | 0.0593 | 0.0028 | 0.0106 | 0.0066 | 0.0647 | 0.0663 | 0.0085 | 0.0081 | 0.0025 | 0.0156 | 0.0398 | 0.0157 | 0.0101 | 0.001 |
Bud Site | 0.0139 | 0.0801 | 0.031 | 0.0104 | 0.0026 | 0.0018 | 0.0614 | 0.2161 | 0.0527 | 0.0259 | 0.0059 | 0.0065 | 0.0175 | 0.1268 | 0.0386 | 0.0181 | 0.0057 | 0.0012 |
Cell Periphery | 0.001 | 0.0012 | 0.0006 | 0.001 | 0.0007 | 0.0006 | 0.001 | 0.0014 | 0.0011 | 0.0013 | 0.0002 | 0.0007 | 0.0003 | 0.001 | 0.0004 | 0.0003 | 0.0009 | 0.0002 |
Cytoplasm | 0.0345 | 0.0748 | 0.0158 | 0.0366 | 0.0456 | 0.0183 | 0.0086 | 0.0375 | 0.0235 | 0.0095 | 0.0856 | 0.0088 | 0.0045 | 0.0907 | 0.023 | 0.0117 | 0.0574 | 0.0095 |
Cytoplasmic Foci | 0.0232 | 0.0873 | 0.0098 | 0.0124 | 0.0651 | 0.0215 | 0.0414 | 0.0313 | 0.0095 | 0.0362 | 0.0271 | 0.0129 | 0.0317 | 0.0479 | 0.0214 | 0.0346 | 0.0754 | 0.0208 |
Eisosomes | 0.0008 | 0.0014 | 0.0004 | 0.0005 | 0.0003 | 0.0007 | 0.0013 | 0.0011 | 0.0003 | 0.0005 | 0.0003 | 0.0006 | 0.0005 | 0.0002 | 0.0003 | 0.0003 | 0.0002 | 0.0005 |
Endoplasmic Reticulum | 0.0077 | 0.0014 | 0.0005 | 0.0012 | 0.0005 | 0.0018 | 0.0011 | 0.0003 | 0.0004 | 0.0007 | 0.0045 | 0.0009 | 0.0037 | 0.0008 | 0.0002 | 0.0016 | 0.0009 | 0.0004 |
Endosome | 0.0211 | 0.0204 | 0.015 | 0.0328 | 0.022 | 0.0264 | 0.0123 | 0.0032 | 0.0083 | 0.0249 | 0.0244 | 0.0054 | 0.0227 | 0.011 | 0.0076 | 0.0265 | 0.0343 | 0.0072 |
Golgi | 0.0328 | 0.0295 | 0.0155 | 0.0233 | 0.0404 | 0.026 | 0.0115 | 0.0055 | 0.0092 | 0.0127 | 0.0249 | 0.0078 | 0.0202 | 0.018 | 0.0115 | 0.0203 | 0.0268 | 0.0124 |
Lipid Particles | 0.0092 | 0.0032 | 0.0025 | 0.0116 | 0.0085 | 0.006 | 0.006 | 0.0008 | 0.0007 | 0.0124 | 0.0017 | 0.0005 | 0.0082 | 0.0005 | 0.0026 | 0.0014 | 0.0027 | 0.0014 |
Mitochondria | 0.0081 | 0.0132 | 0.0056 | 0.0081 | 0.0722 | 0.0097 | 0.0442 | 0.0006 | 0.0015 | 0.005 | 0.0038 | 0.0018 | 0.0031 | 0.0016 | 0.0024 | 0.0032 | 0.0033 | 0.0012 |
None | 0.0012 | 0.0072 | 0.0005 | 0.0014 | 0.0005 | 0.0013 | 0.0052 | 0.0092 | 0.0055 | 0.0036 | 0.0169 | 0.0005 | 0.0071 | 0.0007 | 0.0002 | 0.0008 | 0.0017 | 0.0008 |
Nuclear Periphery | 0.0023 | 0.0004 | 0.0005 | 0.0006 | 0.0003 | 0.0018 | 0.0028 | 0.0001 | 0.0059 | 0.0068 | 0.0017 | 0.0001 | 0.0132 | 0.0001 | 0.0001 | 0.0034 | 0.0002 | 0.0008 |
Nucleolus | 0.0001 | 0.0002 | 0.0003 | 0.0014 | 0.0001 | 0.0024 | 0.0037 | 0.0001 | 0.0001 | 0.0151 | 0 | 0 | 0.0005 | 0.0002 | 0.0001 | 0.0001 | 0.0002 | 0.0001 |
Nucleus | 0.0003 | 0.0003 | 0.0004 | 0.0008 | 0.0003 | 0.0054 | 0.0053 | 0.0002 | 0.0005 | 0.0102 | 0.0007 | 0.0001 | 0.0015 | 0.0004 | 0.0001 | 0.0014 | 0.0003 | 0.0003 |
Peroxisomes | 0.0195 | 0.0287 | 0.0076 | 0.0128 | 0.0344 | 0.0241 | 0.0327 | 0.0279 | 0.0107 | 0.0118 | 0.0247 | 0.02 | 0.0334 | 0.028 | 0.0168 | 0.0191 | 0.0135 | 0.0178 |
Punctate Nuclear | 0.001 | 0.0012 | 0.0006 | 0.0004 | 0.0011 | 0.0004 | 0.0059 | 0.0003 | 0.0007 | 0.0093 | 0.0006 | 0.0002 | 0.005 | 0.0004 | 0.0005 | 0.0019 | 0.0015 | 0.0015 |
Vacuole | 0.0019 | 0.0007 | 0.0007 | 0.0147 | 0.0009 | 0.0045 | 0.005 | 0.0005 | 0.0005 | 0.0058 | 0.0008 | 0.0002 | 0.0014 | 0.0008 | 0.0005 | 0.0013 | 0.0016 | 0.0002 |
Vacuole Periphery | 0.0009 | 0.0002 | 0.0002 | 0.0026 | 0.0006 | 0.0019 | 0.001 | 0 | 0.0002 | 0.0012 | 0.0005 | 0.0001 | 0.0009 | 0.0002 | 0.0001 | 0.0007 | 0.0011 | 0.0001 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 23.258 | 19.8242 | 17.222 | 22.2559 | 24.1942 | 24.3263 | 31.8997 | 30.1917 | 31.6058 | 24.2989 |
Translational Efficiency | 1.8936 | 1.764 | 1.7028 | 1.8439 | 1.4153 | 1.9851 | 0.9703 | 1.22 | 1.2178 | 1.371 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
426 | 1286 | 249 | 1464 | 1630 | 1634 | 913 | 2187 | 2056 | 2920 | 1162 | 3651 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 834.34 | 910.90 | 1154.36 | 1072.38 | 860.16 | 925.81 | 1203.45 | 1135.94 | 854.81 | 919.24 | 1192.93 | 1110.45 |
Standard Deviation | 100.25 | 159.91 | 206.80 | 156.20 | 103.81 | 128.28 | 142.84 | 160.52 | 103.61 | 143.26 | 160.00 | 161.83 |
Intensity Change Log 2 | — | 0.126657 | 0.468386 | 0.362109 | — | 0.106111 | 0.484499 | 0.401210 | — | 0.116264 | 0.476588 | 0.382090 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.509116* | 0.736084* | 0.606047* | 0.570927* | 0.639329* | 0.619427* | 0.668780* | 0.588905* | 0.612349* | 0.670804* | 0.655338* | 0.581696* |
Bud Neck | 0.022919 | 0.001506 | 0.015848 | 0.007958 | 0.007984 | 0.004266 | 0.007333 | 0.008425 | 0.011079 | 0.003050 | 0.009158 | 0.008238 |
Bud Site | 0.134365* | 0.026595 | 0.196198* | 0.258629* | 0.095173 | 0.092230 | 0.231254* | 0.260770* | 0.103293* | 0.063324 | 0.223742* | 0.259912* |
Cell Periphery | 0.009307 | 0.001212 | 0.001676 | 0.004850 | 0.003076 | 0.001784 | 0.001647 | 0.004885 | 0.004367 | 0.001532 | 0.001654 | 0.004871 |
Cytoplasm | 0.030001 | 0.008930 | 0.009821 | 0.074753 | 0.029453 | 0.042951 | 0.031855 | 0.105976* | 0.029567 | 0.027968 | 0.027134 | 0.093456 |
Cytoplasmic Foci | 0.082391 | 0.027352 | 0.031784 | 0.015272 | 0.039491 | 0.026832 | 0.013723 | 0.003923 | 0.048380 | 0.027061 | 0.017593 | 0.008474 |
Eisosomes | 0.003325 | 0.001724 | 0.001679 | 0.002504 | 0.002470 | 0.000943 | 0.000971 | 0.001984 | 0.002647 | 0.001287 | 0.001123 | 0.002192 |
Endoplasmic Reticulum | 0.000472 | 0.000106 | 0.001465 | 0.000890 | 0.000215 | 0.000072 | 0.000282 | 0.000259 | 0.000269 | 0.000087 | 0.000536 | 0.000512 |
Endosome | 0.005414 | 0.022531 | 0.037413 | 0.006950 | 0.007323 | 0.012903 | 0.003825 | 0.001393 | 0.006928 | 0.017143 | 0.011022 | 0.003621 |
Golgi | 0.011318 | 0.020571 | 0.016836 | 0.007799 | 0.016912 | 0.022252 | 0.002062 | 0.002262 | 0.015753 | 0.021512 | 0.005228 | 0.004482 |
Lipid Particles | 0.001471 | 0.013669 | 0.023476 | 0.001943 | 0.001516 | 0.000944 | 0.000667 | 0.000706 | 0.001506 | 0.006548 | 0.005554 | 0.001202 |
Mitochondria | 0.002562 | 0.000808 | 0.000710 | 0.002837 | 0.003119 | 0.007557 | 0.000158 | 0.001916 | 0.003003 | 0.004585 | 0.000277 | 0.002285 |
Mitotic Spindle | 0.000471 | 0.000539 | 0.008693 | 0.003569 | 0.001082 | 0.002262 | 0.000531 | 0.001159 | 0.000956 | 0.001503 | 0.002280 | 0.002125 |
None | 0.000115 | 0.000027 | 0.000015 | 0.000033 | 0.000050 | 0.000094 | 0.000019 | 0.000027 | 0.000064 | 0.000064 | 0.000018 | 0.000029 |
Nuclear Periphery | 0.000015 | 0.000005 | 0.000030 | 0.000054 | 0.000002 | 0.000003 | 0.000007 | 0.000018 | 0.000005 | 0.000004 | 0.000012 | 0.000033 |
Nuclear Periphery Foci | 0.000574 | 0.000071 | 0.000638 | 0.000380 | 0.000051 | 0.000015 | 0.000162 | 0.000048 | 0.000160 | 0.000039 | 0.000264 | 0.000181 |
Nucleolus | 0.000175 | 0.000012 | 0.000005 | 0.000121 | 0.000013 | 0.000159 | 0.000003 | 0.000003 | 0.000046 | 0.000094 | 0.000003 | 0.000050 |
Nucleus | 0.000342 | 0.000026 | 0.000346 | 0.000721 | 0.000139 | 0.000214 | 0.000229 | 0.000395 | 0.000181 | 0.000131 | 0.000254 | 0.000525 |
Peroxisomes | 0.184988* | 0.137883* | 0.037202 | 0.026856 | 0.151540* | 0.163349* | 0.028107 | 0.012023 | 0.158470* | 0.152133* | 0.030056 | 0.017971 |
Vacuole | 0.000395 | 0.000267 | 0.010003 | 0.010875 | 0.000943 | 0.000800 | 0.008242 | 0.004455 | 0.000829 | 0.000565 | 0.008620 | 0.007030 |
Vacuole Periphery | 0.000264 | 0.000082 | 0.000114 | 0.002079 | 0.000118 | 0.000945 | 0.000142 | 0.000468 | 0.000148 | 0.000565 | 0.000136 | 0.001114 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -11.12 | -2.77 | -2.71 | 12.69 | 1.08 | 1.59 | -1.05 | 4.58 | 2.85 | 4.75 | -5.64 | -2.17 | 3.50 | 10.41 | 5.11 |
Bud Neck | 5.03 | 1.43 | 3.47 | -8.03 | 2.80 | 3.48 | 0.53 | -0.36 | -5.28 | -0.96 | 6.65 | 1.31 | 2.23 | -9.18 | 0.76 |
Bud Site | 9.16 | -2.86 | -8.92 | -27.91* | -3.10 | 0.43 | -11.76 | -20.36 | -21.11 | -2.58 | 7.26 | -11.78 | -23.02 | -33.95* | -3.65 |
Cell Periphery | 3.78 | 3.59 | 2.06 | -5.53 | -5.29 | 2.00 | 2.87 | -2.84 | -5.18 | -7.40 | 4.37 | 4.57 | -0.70 | -7.59 | -9.02 |
Cytoplasm | 4.67 | 3.59 | -8.17 | -15.79 | -13.62 | -3.81 | -1.97 | -17.07 | -13.25 | -12.11 | 0.60 | -0.89 | -18.88 | -19.92 | -14.37 |
Cytoplasmic Foci | 6.68 | 5.26 | 8.20 | 4.48 | 3.18 | 4.21 | 7.56 | 13.96 | 12.52 | 5.30 | 7.33 | 9.07 | 14.74 | 12.12 | 4.95 |
Eisosomes | 1.64 | 1.52 | 0.89 | -1.14 | -1.00 | 4.81 | 4.43 | 1.42 | -5.73 | -4.62 | 3.63 | 4.33 | 1.30 | -2.97 | -3.87 |
Endoplasmic Reticulum | 1.24 | -2.62 | -1.24 | -6.68 | 2.22 | 3.42 | -1.48 | -1.06 | -5.21 | 0.61 | 2.56 | -3.03 | -2.84 | -8.22 | 0.72 |
Endosome | -7.28 | -4.81 | -1.06 | 6.94 | 4.60 | -3.47 | 2.92 | 6.76 | 8.25 | 3.20 | -7.40 | -2.43 | 4.05 | 10.79 | 4.61 |
Golgi | -3.42 | -1.27 | 1.55 | 5.86 | 2.36 | -2.43 | 9.89 | 9.48 | 11.39 | -0.30 | -3.33 | 7.20 | 8.39 | 12.48 | 0.66 |
Lipid Particles | -5.50 | -3.62 | -0.99 | 5.33 | 3.54 | 1.25 | 1.99 | 1.85 | 0.29 | -0.16 | -4.96 | -3.07 | 0.58 | 5.29 | 3.25 |
Mitochondria | 3.31 | 3.30 | -0.26 | -2.55 | -2.61 | -3.72 | 6.13 | 1.94 | 4.86 | -4.27 | -2.16 | 6.81 | 1.34 | 3.18 | -5.02 |
Mitotic Spindle | 0.09 | -2.23 | -3.24 | -3.46 | 1.35 | -1.68 | 1.13 | -0.13 | 1.65 | -1.49 | -1.11 | -1.57 | -2.18 | -1.29 | 0.18 |
None | 3.41 | 4.27 | 3.53 | -0.38 | -2.83 | -0.97 | 1.26 | 2.29 | 1.42 | -0.01 | -0.15 | 2.63 | 3.68 | 1.32 | -0.38 |
Nuclear Periphery | 0.88 | -0.94 | -1.01 | -1.69 | -0.52 | -1.32 | -4.20 | -1.25 | -1.18 | -0.65 | 0.48 | -2.39 | -1.87 | -2.05 | -1.18 |
Nuclear Periphery Foci | 0.94 | -0.02 | 0.53 | -2.08 | 1.69 | 1.67 | -3.54 | -0.12 | -3.04 | 3.71 | 1.03 | -0.90 | 0.12 | -2.41 | 2.11 |
Nucleolus | 1.00 | 1.02 | 0.46 | -0.99 | -1.05 | -1.11 | 1.39 | 1.46 | 1.18 | 0.49 | -0.38 | 1.16 | 0.13 | 0.49 | -1.05 |
Nucleus | 1.28 | -0.62 | -1.04 | -5.81 | -0.29 | -0.45 | -2.58 | -5.83 | -1.24 | 0.06 | 0.55 | -2.30 | -4.15 | -3.97 | -0.65 |
Peroxisomes | 3.42 | 10.58 | 12.70 | 16.89 | 1.36 | -1.50 | 20.16 | 25.09* | 26.09 | 5.28 | 0.96 | 22.68 | 27.35 | 30.87 | 4.13 |
Vacuole | 0.89 | -3.29 | -8.22 | -8.32 | 0.98 | 0.40 | -5.19 | -5.36 | -6.17 | 3.36 | 0.85 | -6.14 | -9.30 | -10.36 | 2.80 |
Vacuole Periphery | 0.86 | 0.70 | -2.39 | -2.73 | -2.69 | -1.50 | -0.20 | -1.74 | 0.82 | -1.42 | -1.33 | 0.11 | -3.02 | -1.23 | -2.97 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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