Standard name | |
---|---|
Human Ortholog | |
Description | Specific chaperone for ribosomal protein Rpl10p; binds nascent Rpl10p during translation; contains multiple WD repeats; interacts with Qsr1p in a two-hybrid assay; protein abundance increases in response to DNA replication stress |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 5 | 0 | 1 | 2 | 2 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 2 | 0 | 1 | 1 |
Bud | 0 | 0 | 0 | 0 | 0 | 1 | 3 | 5 | 1 | 5 | 2 | 3 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 4 | 7 | 24 | 92 | 69 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 3 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 2 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 3 | 1 | 3 | 0 | 1 | 2 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 35 | 192 | 153 | 64 | 77 | 156 | 161 | 186 | 152 | 125 | 109 | 120 | 288 | 334 | 362 | 78 | 87 | 53 | 41 | 79 | 135 | 150 | 201 | 187 |
Endoplasmic Reticulum | 0 | 0 | 3 | 0 | 0 | 1 | 3 | 12 | 4 | 5 | 3 | 6 | 2 | 2 | 1 | 13 | 13 | 9 | 0 | 2 | 5 | 2 | 1 | 6 |
Endosome | 0 | 0 | 0 | 0 | 1 | 0 | 3 | 0 | 0 | 1 | 1 | 0 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 3 | 4 | 43 | 53 |
Golgi | 2 | 0 | 10 | 10 | 25 | 12 | 4 | 23 | 17 | 2 | 4 | 5 | 8 | 37 | 66 | 51 | 45 | 52 | 0 | 0 | 0 | 1 | 1 | 2 |
Mitochondria | 0 | 0 | 1 | 0 | 2 | 19 | 39 | 44 | 23 | 58 | 77 | 51 | 0 | 0 | 1 | 3 | 0 | 2 | 0 | 0 | 2 | 0 | 0 | 2 |
Nucleus | 0 | 6 | 11 | 31 | 23 | 41 | 54 | 63 | 42 | 37 | 46 | 24 | 11 | 36 | 36 | 18 | 4 | 9 | 33 | 105 | 118 | 163 | 235 | 170 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 5 | 2 | 6 | 6 |
Nucleolus | 1 | 1 | 0 | 0 | 1 | 1 | 3 | 4 | 0 | 7 | 4 | 2 | 1 | 1 | 0 | 8 | 14 | 17 | 0 | 0 | 1 | 2 | 12 | 8 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 3 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 5 | 69 | 85 |
Vac/Vac Membrane | 46 | 35 | 201 | 53 | 148 | 144 | 143 | 194 | 97 | 165 | 108 | 74 | 239 | 349 | 298 | 182 | 180 | 133 | 5 | 25 | 47 | 29 | 75 | 60 |
Unique Cell Count | 73 | 216 | 317 | 130 | 243 | 330 | 379 | 480 | 299 | 380 | 315 | 258 | 433 | 607 | 604 | 271 | 272 | 216 | 86 | 227 | 340 | 392 | 759 | 669 |
Labelled Cell Count | 84 | 234 | 379 | 158 | 278 | 376 | 419 | 535 | 338 | 411 | 358 | 289 | 553 | 761 | 766 | 353 | 345 | 275 | 86 | 227 | 340 | 392 | 759 | 669 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 21.7 | 30.7 | 23.9 | 20.3 | 18.7 | 16.1 | 14.7 | 14.8 | 14.2 | 13.6 | 13.3 | 13.0 | 23.8 | 23.0 | 20.3 | 33.7 | 32.1 | 34.9 | 23.2 | 23.7 | 23.1 |
Std Deviation (1e-4) | 4.8 | 5.0 | 5.2 | 5.4 | 4.7 | 3.9 | 3.4 | 3.7 | 3.5 | 3.5 | 3.7 | 3.2 | 8.6 | 8.1 | 7.6 | 11.2 | 10.1 | 9.8 | 8.4 | 8.9 | 9.2 |
Intensity Change (Log2) | – | – | – | -0.24 | -0.35 | -0.57 | -0.7 | -0.69 | -0.75 | -0.81 | -0.84 | -0.88 | -0.0 | -0.05 | -0.23 | 0.5 | 0.43 | 0.55 | -0.04 | -0.01 | -0.05 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0.2 | -4.0 | -0.3 | -1.5 | -2.7 | 0.6 | -4.1 | -3.5 | -0.4 | 5.0 | 2.0 | 3.4 | -4.8 | -4.0 | -5.5 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 1.6 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2.9 | 2.9 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 2.1 | 3.5 | 0.3 | -2.0 | 1.1 | 1.5 | -2.7 | -1.6 | -0.9 | -1.2 | 1.9 | 4.1 | 6.2 | 5.6 | 7.4 |
Mitochondria | 0 | 0 | 4.0 | 5.6 | 5.3 | 4.7 | 7.1 | 9.2 | 8.1 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 6.7 | 2.9 | 4.2 | 4.9 | 4.6 | 4.7 | 3.3 | 4.9 | 2.9 | -0.7 | 1.6 | 1.6 | 1.8 | -1.5 | 0.4 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 4.1 | 5.1 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | -4.4 | -0.6 | -5.0 | -6.7 | -6.4 | -7.7 | -5.3 | -7.3 | -8.3 | -2.3 | -1.7 | -4.1 | 1.0 | 0.7 | -0.4 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 21.6944 | 28.0211 | 24.9157 | 21.6141 | 23.0447 | 24.5591 | 18.6267 | 27.2651 | 23.8677 | 19.585 | 25.2234 | 23.8229 | 25.164 | 29.2112 | 27.9901 | 26.0021 | 26.343 | 26.8448 |
Actin | 0.0112 | 0.0001 | 0.0007 | 0.0008 | 0.0086 | 0.0001 | 0.0274 | 0.0002 | 0.0168 | 0.0298 | 0.0044 | 0.0022 | 0.007 | 0.0002 | 0.001 | 0.0001 | 0.0047 | 0.0009 |
Bud | 0.0009 | 0 | 0.0003 | 0.0002 | 0.0032 | 0.0001 | 0.0013 | 0.0001 | 0.0005 | 0.0045 | 0.0001 | 0.0003 | 0.0003 | 0.0001 | 0.0003 | 0.0002 | 0.0002 | 0.0001 |
Bud Neck | 0.0007 | 0.0002 | 0.0003 | 0.0002 | 0.0004 | 0.0008 | 0.0161 | 0.0002 | 0.0005 | 0.0027 | 0.0006 | 0.0012 | 0.001 | 0.0002 | 0.0002 | 0.0004 | 0.0005 | 0.0007 |
Bud Periphery | 0.0005 | 0 | 0.0001 | 0.0001 | 0.0048 | 0.0001 | 0.0015 | 0 | 0.0006 | 0.0109 | 0.0001 | 0.0002 | 0.0002 | 0 | 0.0003 | 0.0001 | 0.0001 | 0.0002 |
Bud Site | 0.0016 | 0.0001 | 0.0002 | 0.0002 | 0.0139 | 0 | 0.01 | 0.0001 | 0.0005 | 0.0116 | 0.0003 | 0.0004 | 0.0008 | 0.0001 | 0.0031 | 0.0001 | 0.0003 | 0.0001 |
Cell Periphery | 0.0001 | 0 | 0 | 0.0001 | 0.0002 | 0 | 0.0005 | 0 | 0.0001 | 0.0011 | 0 | 0 | 0.0001 | 0 | 0 | 0.0001 | 0.0001 | 0.0001 |
Cytoplasm | 0.3836 | 0.5813 | 0.5041 | 0.4783 | 0.4656 | 0.4044 | 0.2438 | 0.4578 | 0.3166 | 0.2568 | 0.4832 | 0.3032 | 0.2696 | 0.4213 | 0.322 | 0.2986 | 0.3374 | 0.2253 |
Cytoplasmic Foci | 0.0055 | 0.0001 | 0.0006 | 0.0049 | 0.0034 | 0.0001 | 0.0305 | 0.0004 | 0.0063 | 0.0218 | 0.0105 | 0.0015 | 0.0125 | 0.0002 | 0.001 | 0.0006 | 0.0022 | 0.0002 |
Eisosomes | 0.0001 | 0 | 0 | 0 | 0.0001 | 0 | 0.0003 | 0 | 0.0001 | 0.0003 | 0 | 0 | 0.0001 | 0 | 0 | 0 | 0.0001 | 0 |
Endoplasmic Reticulum | 0.0044 | 0.0002 | 0.0011 | 0.006 | 0.0014 | 0.0016 | 0.008 | 0.0003 | 0.0013 | 0.0059 | 0.0005 | 0.0007 | 0.0039 | 0.0006 | 0.0006 | 0.0011 | 0.0024 | 0.0016 |
Endosome | 0.0042 | 0.0001 | 0.0005 | 0.0103 | 0.0037 | 0.0006 | 0.0257 | 0.0001 | 0.0094 | 0.0157 | 0.0068 | 0.0013 | 0.0108 | 0.0001 | 0.0005 | 0.0007 | 0.0022 | 0.0021 |
Golgi | 0.0011 | 0 | 0.0001 | 0.0007 | 0.0018 | 0 | 0.0069 | 0.0001 | 0.0015 | 0.011 | 0.0058 | 0.0011 | 0.0017 | 0 | 0.0001 | 0 | 0.0021 | 0.0001 |
Lipid Particles | 0.0016 | 0 | 0.0003 | 0.0013 | 0.0039 | 0 | 0.016 | 0.0001 | 0.0042 | 0.0196 | 0.0181 | 0.0035 | 0.006 | 0 | 0.0002 | 0.0001 | 0.012 | 0.0001 |
Mitochondria | 0.0025 | 0.0001 | 0.0005 | 0.0011 | 0.0015 | 0.0003 | 0.0059 | 0.0003 | 0.0025 | 0.0324 | 0.0011 | 0.0009 | 0.0036 | 0.0002 | 0.0003 | 0.0003 | 0.0053 | 0.0025 |
None | 0.0029 | 0.0001 | 0.0004 | 0.0134 | 0.0008 | 0.0001 | 0.0106 | 0.0001 | 0.0086 | 0.0043 | 0.0003 | 0.0002 | 0.0036 | 0.0002 | 0.0005 | 0.0006 | 0.0011 | 0.0002 |
Nuclear Periphery | 0.0458 | 0.0154 | 0.0246 | 0.0284 | 0.0183 | 0.0383 | 0.0429 | 0.018 | 0.0213 | 0.0229 | 0.0227 | 0.0295 | 0.0365 | 0.0207 | 0.0185 | 0.022 | 0.0337 | 0.0302 |
Nucleolus | 0.0005 | 0.0001 | 0.0003 | 0.0002 | 0.0007 | 0.0002 | 0.0044 | 0.0007 | 0.0003 | 0.0015 | 0.0001 | 0.0007 | 0.0016 | 0.0003 | 0.0004 | 0.0012 | 0.0004 | 0.0003 |
Nucleus | 0.524 | 0.4011 | 0.4628 | 0.4473 | 0.4609 | 0.5493 | 0.4931 | 0.5173 | 0.5968 | 0.5086 | 0.4338 | 0.6464 | 0.6207 | 0.554 | 0.6479 | 0.6674 | 0.5913 | 0.7326 |
Peroxisomes | 0.0004 | 0 | 0 | 0.0001 | 0.002 | 0 | 0.0142 | 0.0001 | 0.0058 | 0.0154 | 0.0084 | 0.0011 | 0.0017 | 0 | 0.0002 | 0 | 0.0007 | 0 |
Punctate Nuclear | 0.0055 | 0.0006 | 0.0016 | 0.0013 | 0.0029 | 0.0007 | 0.0323 | 0.0037 | 0.0027 | 0.0085 | 0.0025 | 0.0034 | 0.0101 | 0.0012 | 0.0022 | 0.0032 | 0.0022 | 0.001 |
Vacuole | 0.002 | 0.0003 | 0.001 | 0.0037 | 0.0014 | 0.0022 | 0.0061 | 0.0003 | 0.0025 | 0.0096 | 0.0005 | 0.0016 | 0.0062 | 0.0005 | 0.0004 | 0.0026 | 0.0007 | 0.0011 |
Vacuole Periphery | 0.0009 | 0.0001 | 0.0004 | 0.0013 | 0.0004 | 0.0008 | 0.0026 | 0.0001 | 0.0009 | 0.005 | 0.0002 | 0.0004 | 0.002 | 0.0001 | 0.0001 | 0.0007 | 0.0004 | 0.0005 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 83.4364 | 85.2424 | 79.0669 | 75.1898 | 94.3807 | 58.7135 | 79.0702 | 82.5826 | 72.5236 | 79.1672 |
Translational Efficiency | 1.9346 | 1.9883 | 1.716 | 1.7925 | 1.358 | 1.9107 | 1.6138 | 1.1302 | 1.2559 | 1.3558 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
2018 | 939 | 2921 | 1213 | 1357 | 1677 | 1348 | 2759 | 3375 | 2616 | 4269 | 3972 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 1620.34 | 2031.39 | 2287.85 | 2581.34 | 1629.29 | 1868.62 | 2303.68 | 2828.68 | 1623.94 | 1927.05 | 2292.85 | 2753.15 |
Standard Deviation | 289.87 | 350.74 | 382.50 | 597.64 | 280.75 | 331.68 | 419.16 | 527.26 | 286.27 | 347.53 | 394.51 | 561.39 |
Intensity Change Log 2 | — | 0.326171 | 0.497696 | 0.671824 | — | 0.197730 | 0.499697 | 0.795886 | — | 0.263202 | 0.498700 | 0.735358 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000125 | 0.000282 | 0.000069 | 0.001788 | 0.000182 | 0.000216 | 0.000271 | 0.000452 | 0.000148 | 0.000240 | 0.000133 | 0.000860 |
Bud Neck | 0.001841 | 0.002720 | 0.002377 | 0.029129 | 0.002784 | 0.002176 | 0.003771 | 0.023454 | 0.002220 | 0.002371 | 0.002817 | 0.025187 |
Bud Site | 0.000523 | 0.002110 | 0.000319 | 0.023422 | 0.001142 | 0.001369 | 0.000950 | 0.011418 | 0.000772 | 0.001635 | 0.000518 | 0.015084 |
Cell Periphery | 0.000071 | 0.000091 | 0.000046 | 0.000671 | 0.000093 | 0.000080 | 0.000144 | 0.000239 | 0.000080 | 0.000084 | 0.000076 | 0.000371 |
Cytoplasm | 0.251507* | 0.104096* | 0.129917* | 0.069653 | 0.192028* | 0.146995* | 0.038439 | 0.073212 | 0.227592* | 0.131597* | 0.101032* | 0.072125 |
Cytoplasmic Foci | 0.000663 | 0.001361 | 0.000082 | 0.008697 | 0.001216 | 0.001019 | 0.000424 | 0.005010 | 0.000885 | 0.001141 | 0.000190 | 0.006136 |
Eisosomes | 0.000002 | 0.000004 | 0.000002 | 0.000018 | 0.000003 | 0.000003 | 0.000007 | 0.000008 | 0.000002 | 0.000003 | 0.000003 | 0.000011 |
Endoplasmic Reticulum | 0.000423 | 0.000438 | 0.000186 | 0.001987 | 0.000966 | 0.000425 | 0.000352 | 0.000441 | 0.000641 | 0.000430 | 0.000238 | 0.000913 |
Endosome | 0.000116 | 0.000251 | 0.000033 | 0.008608 | 0.000144 | 0.000342 | 0.000078 | 0.000507 | 0.000127 | 0.000309 | 0.000047 | 0.002981 |
Golgi | 0.000125 | 0.000506 | 0.000026 | 0.008646 | 0.000327 | 0.000302 | 0.000057 | 0.002707 | 0.000206 | 0.000375 | 0.000036 | 0.004520 |
Lipid Particles | 0.000060 | 0.000197 | 0.000031 | 0.001925 | 0.000099 | 0.000153 | 0.000065 | 0.000156 | 0.000076 | 0.000169 | 0.000042 | 0.000696 |
Mitochondria | 0.000524 | 0.000538 | 0.000775 | 0.004586 | 0.000484 | 0.001069 | 0.001116 | 0.002220 | 0.000508 | 0.000878 | 0.000883 | 0.002942 |
Mitotic Spindle | 0.000448 | 0.003586 | 0.004602 | 0.094022 | 0.001196 | 0.006233 | 0.002698 | 0.095755 | 0.000749 | 0.005283 | 0.004001 | 0.095226 |
None | 0.005994 | 0.005697 | 0.008437 | 0.015169 | 0.004504 | 0.007235 | 0.005327 | 0.016698 | 0.005395 | 0.006683 | 0.007455 | 0.016231 |
Nuclear Periphery | 0.000321 | 0.000351 | 0.000613 | 0.001559 | 0.000547 | 0.000459 | 0.000520 | 0.001384 | 0.000412 | 0.000420 | 0.000584 | 0.001438 |
Nuclear Periphery Foci | 0.000268 | 0.000554 | 0.000036 | 0.003106 | 0.000878 | 0.001420 | 0.000090 | 0.000881 | 0.000514 | 0.001109 | 0.000053 | 0.001561 |
Nucleolus | 0.000118 | 0.000463 | 0.000119 | 0.001349 | 0.000171 | 0.000439 | 0.000176 | 0.003618 | 0.000140 | 0.000447 | 0.000137 | 0.002925 |
Nucleus | 0.735187* | 0.873668* | 0.850916* | 0.702880* | 0.789979* | 0.827037* | 0.943510* | 0.759713* | 0.757217* | 0.843775* | 0.880154* | 0.742357* |
Peroxisomes | 0.000042 | 0.000060 | 0.000007 | 0.000895 | 0.000017 | 0.000053 | 0.000021 | 0.000422 | 0.000032 | 0.000056 | 0.000011 | 0.000566 |
Vacuole | 0.001598 | 0.002727 | 0.001382 | 0.013908 | 0.002894 | 0.002655 | 0.001950 | 0.001442 | 0.002119 | 0.002681 | 0.001561 | 0.005249 |
Vacuole Periphery | 0.000045 | 0.000301 | 0.000025 | 0.007983 | 0.000345 | 0.000322 | 0.000034 | 0.000263 | 0.000165 | 0.000314 | 0.000028 | 0.002620 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -4.39 | 0.93 | -6.88 | -4.90 | -6.99 | -2.34 | -11.11 | -13.09 | -8.88 | -3.69 | -5.34 | -6.34 | -12.88 | -8.75 | -11.18 |
Bud Neck | -3.72 | -5.03 | -18.22 | -15.51 | -17.62 | -1.72 | -7.20 | -30.77 | -29.08 | -21.11 | -4.33 | -8.45 | -35.90 | -32.46 | -32.09* |
Bud Site | -3.25 | -0.56 | -15.56 | -12.21 | -15.54 | -2.06 | -4.66 | -19.60 | -17.51 | -15.05 | -4.24 | -4.06 | -25.83 | -20.91 | -24.52 |
Cell Periphery | -4.22 | 1.87 | -16.69 | -15.05 | -17.15 | -0.40 | -10.95 | -14.45 | -12.65 | -0.42 | -3.39 | -6.40 | -21.96 | -19.17 | -18.75 |
Cytoplasm | 23.27 | 26.31 | 37.67 | 10.98 | 18.12 | 7.26 | 26.49 | 25.47 | 25.73 | -3.48 | 21.04 | 34.24 | 47.23 | 27.55 | 20.53 |
Cytoplasmic Foci | -2.32 | 4.14 | -9.50 | -8.15 | -9.70 | -0.93 | -2.17 | -7.81 | -7.22 | -5.02 | -2.79 | -0.18 | -12.33 | -10.54 | -12.11 |
Eisosomes | -4.56 | -5.34 | -23.57 | -19.92 | -22.72 | -1.23 | -14.58 | -32.38* | -29.73* | -4.97 | -4.90 | -14.50 | -39.68* | -34.28* | -28.00 |
Endoplasmic Reticulum | -0.50 | 3.65 | -4.85 | -3.74 | -6.42 | 6.44 | 5.16 | 3.37 | -6.46 | -4.45 | 3.46 | 6.99 | -3.03 | -6.50 | -11.28 |
Endosome | -1.55 | 0.85 | -4.15 | -3.65 | -4.40 | -2.02 | 0.55 | -13.92 | -1.90 | -12.25 | -2.44 | 1.14 | -6.31 | -4.18 | -7.21 |
Golgi | -1.44 | 0.79 | -2.73 | -2.05 | -2.87 | 0.05 | 0.53 | -3.13 | -4.13 | -4.75 | -1.15 | 0.96 | -4.74 | -4.22 | -5.54 |
Lipid Particles | -2.37 | -0.60 | -7.88 | -6.10 | -7.84 | -2.88 | -3.99 | -9.26 | -4.33 | -2.48 | -3.89 | -3.44 | -11.23 | -7.29 | -9.91 |
Mitochondria | -0.81 | -3.88 | -10.07 | -11.12 | -9.28 | -1.30 | -22.35 | -29.66* | -4.63 | -13.82 | -1.37 | -8.59 | -20.37 | -7.87 | -18.83 |
Mitotic Spindle | -2.62 | -5.95 | -13.26 | -12.03 | -11.86 | -3.88 | -4.46 | -27.45* | -25.07* | -24.08* | -4.83 | -7.38 | -30.35 | -27.73 | -27.64 |
None | -0.36 | -9.14 | -11.65 | -10.82 | -7.65 | -4.51 | -3.50 | -31.80* | -25.14* | -28.73* | -3.40 | -10.19 | -32.93* | -27.51* | -26.96* |
Nuclear Periphery | -1.87 | -15.55 | -20.22 | -17.92 | -15.68 | 0.06 | -7.83 | -17.52 | -15.73 | -13.91 | -1.41 | -15.85 | -25.61 | -21.92 | -19.95 |
Nuclear Periphery Foci | -1.85 | 2.74 | -6.10 | -4.86 | -6.66 | -0.93 | 1.83 | -3.94 | -1.24 | -8.14 | -1.78 | 3.09 | -8.61 | -3.24 | -11.65 |
Nucleolus | -3.56 | -2.46 | -11.98 | -10.99 | -11.56 | -2.87 | -4.59 | -25.49* | -25.16* | -23.33* | -4.62 | -5.15 | -27.02* | -26.49* | -26.07* |
Nucleus | -16.29 | -21.86 | 5.44 | 16.05 | 17.85 | -4.50 | -16.45 | 19.13 | 25.46 | 37.45 | -14.82 | -26.18 | 18.44 | 30.42 | 40.09 |
Peroxisomes | -1.45 | 0.81 | -7.43 | -6.92 | -7.99 | -3.31 | -3.69 | -12.83 | -10.48 | -7.11 | -2.51 | -1.59 | -13.59 | -12.46 | -13.21 |
Vacuole | -3.63 | -5.49 | -13.33 | -9.90 | -11.91 | -3.02 | -5.11 | -6.00 | -2.16 | 1.21 | -5.39 | -7.16 | -14.95 | -8.84 | -9.66 |
Vacuole Periphery | -1.89 | 0.83 | -2.68 | -2.07 | -2.70 | 0.17 | 1.09 | 0.53 | 0.90 | -8.69 | -0.98 | 1.17 | -2.10 | -1.49 | -3.50 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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