Standard name | |
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Human Ortholog | |
Description | Integral membrane protein of the Golgi; required for targeting of the Arf-like GTPase Arl3p to the Golgi; multicopy suppressor of ypt6 null mutation |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 3 | 7 | 2 | 0 | 1 | 3 | 1 | 0 | – | 0 | 1 | 1 | 17 | 12 | 4 | 6 | 7 | 8 | 4 | 3 | 2 | 6 | 7 | 11 |
Bud | 3 | 5 | 4 | 7 | 8 | 17 | 31 | 20 | – | 17 | 18 | 5 | 3 | 1 | 3 | 0 | 0 | 1 | 0 | 1 | 0 | 1 | 1 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 1 | 2 | 4 | 8 | – | 7 | 5 | 6 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 2 | 1 | 1 | 0 | 0 | 1 | 0 | 2 | – | 1 | 0 | 1 | 1 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 15 | 17 | 5 | 16 | 31 | 30 | 31 | 33 | – | 19 | 33 | 20 | 6 | 9 | 16 | 7 | 11 | 4 | 0 | 1 | 1 | 1 | 0 | 1 |
Endoplasmic Reticulum | 3 | 5 | 5 | 1 | 4 | 15 | 4 | 5 | – | 5 | 0 | 3 | 6 | 13 | 7 | 4 | 2 | 2 | 0 | 0 | 0 | 1 | 3 | 5 |
Endosome | 61 | 42 | 9 | 28 | 51 | 3 | 5 | 13 | – | 3 | 1 | 4 | 34 | 49 | 67 | 15 | 9 | 7 | 67 | 47 | 42 | 47 | 52 | 42 |
Golgi | 84 | 76 | 11 | 12 | 4 | 0 | 1 | 0 | – | 0 | 1 | 1 | 47 | 119 | 112 | 49 | 52 | 46 | 171 | 142 | 75 | 115 | 129 | 134 |
Mitochondria | 169 | 127 | 131 | 154 | 197 | 241 | 377 | 335 | – | 265 | 240 | 246 | 60 | 58 | 52 | 2 | 4 | 3 | 24 | 28 | 20 | 45 | 45 | 37 |
Nucleus | 0 | 0 | 0 | 1 | 5 | 2 | 2 | 4 | – | 3 | 0 | 1 | 1 | 1 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 |
Nuclear Periphery | 0 | 1 | 2 | 0 | 0 | 0 | 1 | 0 | – | 1 | 0 | 1 | 1 | 2 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 2 | 3 | 4 | 3 | 0 | 3 | 6 | 10 | – | 5 | 8 | 8 | 1 | 2 | 2 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 1 | 4 | 0 | 6 | 11 | 6 | 9 | 11 | – | 5 | 6 | 0 | 5 | 6 | 5 | 0 | 0 | 0 | 19 | 9 | 14 | 16 | 18 | 10 |
SpindlePole | 8 | 5 | 2 | 4 | 14 | 6 | 14 | 21 | – | 10 | 5 | 5 | 8 | 8 | 11 | 1 | 0 | 2 | 1 | 1 | 0 | 1 | 0 | 1 |
Vac/Vac Membrane | 28 | 18 | 4 | 7 | 17 | 6 | 11 | 19 | – | 16 | 11 | 8 | 14 | 24 | 30 | 12 | 10 | 11 | 6 | 5 | 1 | 7 | 5 | 9 |
Unique Cell Count | 261 | 215 | 149 | 197 | 270 | 278 | 423 | 396 | 295 | 272 | 271 | 149 | 233 | 254 | 82 | 80 | 67 | 298 | 244 | 162 | 251 | 266 | 259 | |
Labelled Cell Count | 379 | 311 | 180 | 239 | 344 | 335 | 497 | 481 | 358 | 329 | 310 | 204 | 304 | 313 | 97 | 96 | 85 | 298 | 244 | 162 | 251 | 266 | 259 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 7.6 | 7.6 | 5.4 | 5.7 | 5.4 | 5.3 | 4.8 | 5.1 | – | 4.5 | 5.0 | 4.7 | 7.6 | 7.8 | 7.6 | 10.3 | 10.0 | 10.6 | 8.1 | 8.1 | 8.6 |
Std Deviation (1e-4) | 1.2 | 1.2 | 1.1 | 1.1 | 1.5 | 1.4 | 1.6 | 1.5 | – | 1.0 | 1.5 | 1.0 | 1.1 | 1.2 | 1.2 | 1.6 | 1.8 | 1.6 | 1.3 | 1.2 | 1.6 |
Intensity Change (Log2) | – | – | – | 0.07 | 0.01 | -0.04 | -0.17 | -0.08 | – | -0.26 | -0.11 | -0.19 | 0.5 | 0.53 | 0.49 | 0.93 | 0.89 | 0.97 | 0.58 | 0.58 | 0.66 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 3.6 | 1.9 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 1.6 | 0 | 1.2 | – | 0 | 1.7 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 1.8 | 2.8 | 2.7 | 1.7 | 2.0 | – | 1.4 | 3.0 | 1.7 | 0.3 | 0.3 | 1.3 | 0 | 2.9 | 0 |
Endoplasmic Reticulum | 0 | 0 | 1.0 | 0 | 0 | – | 0 | 0 | 0 | 0.3 | 1.0 | 0 | 0 | 0 | 0 |
Endosome | 2.4 | 3.6 | 0 | 0 | -1.5 | – | 0 | 0 | 0 | 4.1 | 4.0 | 5.0 | 2.9 | 1.4 | 0 |
Golgi | -0.5 | -3.1 | 0 | 0 | 0 | – | 0 | 0 | 0 | 5.3 | 8.8 | 7.7 | 8.7 | 9.3 | 9.5 |
Mitochondria | -2.4 | -3.6 | -0.4 | 0 | -1.0 | – | 0 | 0.1 | 0 | -8.6 | -12.0 | -13.1 | -12.6 | -12.2 | -11.7 |
Nucleus | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 2.0 | 0 | 0 | 2.0 | – | 0 | 0 | 0 | 1.9 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 1.6 | 0 | 0 | 1.1 | – | 1.3 | 0.7 | 0 | 2.4 | 2.8 | 3.2 | 3.4 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 3.2605 | 3.5425 | 3.3121 | 2.7314 | 3.1357 | 3.3529 | 4.2893 | 5.3098 | 4.7932 | 4.726 | 4.3072 | 4.9411 | 1.9734 | 3.2583 | 2.4086 | 2.7528 | 2.3231 | 2.6863 |
Actin | 0.0098 | 0.0051 | 0.0076 | 0.0007 | 0.0026 | 0.0219 | 0.0256 | 0.0121 | 0.0056 | 0.0004 | 0.0232 | 0.0148 | 0.0081 | 0.0018 | 0.0066 | 0.0064 | 0.0011 | 0.0106 |
Bud | 0.0001 | 0.0001 | 0.0002 | 0 | 0 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0002 | 0 | 0 |
Bud Neck | 0.0003 | 0.0002 | 0.0003 | 0.0001 | 0.0002 | 0.0003 | 0.0008 | 0.0002 | 0.0002 | 0.0001 | 0.0004 | 0.0002 | 0.0003 | 0.0002 | 0.0001 | 0.0002 | 0.0001 | 0.0002 |
Bud Periphery | 0.0002 | 0.0001 | 0.0001 | 0 | 0 | 0.0001 | 0.0003 | 0.0001 | 0.0001 | 0 | 0.0002 | 0.0001 | 0.0001 | 0 | 0 | 0.0001 | 0 | 0.0001 |
Bud Site | 0.0005 | 0.0002 | 0.0005 | 0.0001 | 0.0001 | 0.0001 | 0.0031 | 0.0003 | 0.0002 | 0 | 0.0009 | 0.0002 | 0.0002 | 0.0002 | 0.0001 | 0.0005 | 0.0001 | 0.0001 |
Cell Periphery | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0.0003 | 0 | 0 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0.0011 | 0.0014 | 0.0019 | 0.0007 | 0.0009 | 0.0009 | 0.002 | 0.0014 | 0.0009 | 0.0005 | 0.0009 | 0.0009 | 0.0016 | 0.0019 | 0.0011 | 0.0013 | 0.0006 | 0.0006 |
Cytoplasmic Foci | 0.1228 | 0.1384 | 0.1255 | 0.0964 | 0.1321 | 0.1017 | 0.1484 | 0.1464 | 0.1096 | 0.1299 | 0.1067 | 0.0834 | 0.1203 | 0.1488 | 0.1139 | 0.0959 | 0.1139 | 0.0909 |
Eisosomes | 0.0001 | 0 | 0.0001 | 0 | 0 | 0 | 0.0008 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0.0004 | 0 | 0 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0.0007 | 0.0002 | 0.0003 | 0.0002 | 0.0002 | 0.0002 | 0.0017 | 0.0002 | 0.0003 | 0.0001 | 0.0007 | 0.0004 | 0.0006 | 0.0003 | 0.0003 | 0.0004 | 0.0001 | 0.0003 |
Endosome | 0.2143 | 0.2102 | 0.2109 | 0.2828 | 0.247 | 0.1418 | 0.1724 | 0.1544 | 0.1767 | 0.2486 | 0.2284 | 0.138 | 0.2149 | 0.1966 | 0.2071 | 0.2389 | 0.1875 | 0.1339 |
Golgi | 0.4721 | 0.5596 | 0.5642 | 0.572 | 0.5474 | 0.5863 | 0.4134 | 0.5676 | 0.5255 | 0.5484 | 0.3304 | 0.5651 | 0.4759 | 0.5809 | 0.5441 | 0.5579 | 0.5784 | 0.6235 |
Lipid Particles | 0.0461 | 0.0046 | 0.0122 | 0.0183 | 0.0151 | 0.0493 | 0.0701 | 0.0325 | 0.0282 | 0.0081 | 0.2183 | 0.0362 | 0.0434 | 0.0282 | 0.0216 | 0.015 | 0.0122 | 0.0271 |
Mitochondria | 0.0652 | 0.0357 | 0.0326 | 0.0087 | 0.0033 | 0.0274 | 0.028 | 0.0259 | 0.0444 | 0.0048 | 0.0056 | 0.0358 | 0.0413 | 0.005 | 0.0302 | 0.0256 | 0.0337 | 0.0325 |
None | 0.0003 | 0 | 0.0008 | 0 | 0.0001 | 0.0001 | 0.0139 | 0.0002 | 0.0008 | 0 | 0.0014 | 0.0001 | 0.0158 | 0.0002 | 0.0002 | 0.0001 | 0 | 0 |
Nuclear Periphery | 0.001 | 0 | 0.0006 | 0 | 0.0001 | 0.0001 | 0.0021 | 0 | 0.0006 | 0 | 0.0018 | 0.0015 | 0.0017 | 0.0001 | 0.0001 | 0.0001 | 0 | 0 |
Nucleolus | 0.0001 | 0 | 0.0008 | 0 | 0 | 0 | 0.0008 | 0 | 0.0001 | 0 | 0.0004 | 0.0003 | 0.001 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0.0002 | 0 | 0.0004 | 0 | 0 | 0 | 0.001 | 0 | 0.0001 | 0 | 0.0003 | 0.0052 | 0.0012 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0.0609 | 0.0414 | 0.0345 | 0.017 | 0.0481 | 0.068 | 0.107 | 0.0571 | 0.1028 | 0.0581 | 0.0686 | 0.1144 | 0.0685 | 0.0342 | 0.0726 | 0.0543 | 0.0707 | 0.0784 |
Punctate Nuclear | 0.0006 | 0.0001 | 0.0047 | 0.0001 | 0.0002 | 0.0002 | 0.0058 | 0.0002 | 0.0016 | 0.0001 | 0.0085 | 0.0003 | 0.0024 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0001 |
Vacuole | 0.0016 | 0.0014 | 0.0007 | 0.0011 | 0.0014 | 0.0005 | 0.0009 | 0.0004 | 0.0009 | 0.0003 | 0.0012 | 0.0011 | 0.0011 | 0.0005 | 0.0007 | 0.0015 | 0.0005 | 0.0005 |
Vacuole Periphery | 0.002 | 0.0011 | 0.0011 | 0.0017 | 0.0013 | 0.0009 | 0.0012 | 0.0007 | 0.0013 | 0.0006 | 0.0016 | 0.0018 | 0.0013 | 0.0007 | 0.001 | 0.0017 | 0.0009 | 0.001 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 33.3545 | 29.0111 | 27.5073 | 28.7513 | 14.0256 | 36.039 | 36.6663 | 30.2467 | 30.7159 | 37.383 |
Translational Efficiency | 1.0012 | 1.0356 | 0.8302 | 1.0819 | 1.9729 | 0.9551 | 0.8873 | 0.8306 | 1.0682 | 0.788 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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