Standard name | |
---|---|
Human Ortholog | |
Description | GTPase-activating protein (GAP) for yeast Rab family member Ypt6p; involved in vesicle mediated protein transport |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 10 | 4 | 7 | 2 | 2 | 3 | 0 | 5 | 5 | 4 | 3 | 1 | 8 | 22 | 14 | 24 | 8 | 14 | 21 | 7 | 6 | 24 | 37 | 56 |
Bud | 15 | 4 | 2 | 2 | 4 | 3 | 8 | 12 | 9 | 9 | 6 | 11 | 3 | 13 | 1 | 4 | 3 | 2 | 2 | 3 | 0 | 3 | 2 | 8 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 3 | 1 | 0 | 2 | 6 | 8 |
Bud Site | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 1 | 1 | 2 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 2 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 2 | 1 | 1 | 3 | 1 | 4 | 1 | 3 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 |
Cytoplasm | 78 | 20 | 5 | 35 | 23 | 26 | 51 | 60 | 49 | 53 | 41 | 51 | 113 | 232 | 189 | 64 | 41 | 18 | 26 | 13 | 6 | 63 | 122 | 113 |
Endoplasmic Reticulum | 5 | 4 | 2 | 2 | 0 | 4 | 4 | 12 | 19 | 10 | 5 | 9 | 22 | 25 | 24 | 11 | 4 | 5 | 9 | 1 | 2 | 11 | 27 | 64 |
Endosome | 220 | 17 | 3 | 3 | 15 | 16 | 16 | 26 | 7 | 6 | 4 | 7 | 44 | 77 | 107 | 82 | 68 | 20 | 199 | 40 | 32 | 87 | 129 | 131 |
Golgi | 7 | 12 | 1 | 0 | 0 | 2 | 1 | 0 | 2 | 1 | 0 | 1 | 5 | 14 | 29 | 12 | 13 | 18 | 132 | 20 | 10 | 34 | 57 | 86 |
Mitochondria | 146 | 12 | 25 | 25 | 14 | 61 | 99 | 115 | 169 | 153 | 113 | 161 | 9 | 21 | 17 | 32 | 18 | 8 | 35 | 4 | 11 | 30 | 34 | 48 |
Nucleus | 5 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 1 | 1 | 2 |
Nucleolus | 7 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 2 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 |
Peroxisomes | 16 | 0 | 0 | 0 | 0 | 2 | 2 | 8 | 0 | 2 | 0 | 2 | 0 | 3 | 1 | 0 | 2 | 0 | 11 | 1 | 3 | 2 | 2 | 4 |
SpindlePole | 9 | 0 | 2 | 0 | 4 | 1 | 6 | 14 | 5 | 7 | 0 | 1 | 0 | 5 | 6 | 42 | 23 | 5 | 12 | 3 | 4 | 7 | 10 | 13 |
Vac/Vac Membrane | 11 | 3 | 1 | 1 | 1 | 1 | 5 | 7 | 1 | 7 | 1 | 4 | 3 | 10 | 16 | 8 | 13 | 4 | 10 | 7 | 2 | 10 | 19 | 32 |
Unique Cell Count | 415 | 58 | 39 | 61 | 52 | 96 | 172 | 206 | 222 | 207 | 142 | 204 | 196 | 381 | 372 | 223 | 154 | 74 | 475 | 107 | 84 | 285 | 461 | 585 |
Labelled Cell Count | 531 | 78 | 49 | 71 | 63 | 120 | 195 | 262 | 269 | 257 | 174 | 253 | 208 | 428 | 406 | 283 | 195 | 96 | 475 | 107 | 84 | 285 | 461 | 585 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 7.0 | 7.8 | 5.9 | 5.5 | 6.0 | 5.0 | 4.7 | 4.9 | 4.5 | 4.5 | 4.4 | 4.3 | 7.9 | 7.7 | 7.8 | 7.1 | 8.5 | 9.7 | 7.7 | 7.7 | 8.5 |
Std Deviation (1e-4) | 0.9 | 1.1 | 0.8 | 0.7 | 1.3 | 0.7 | 1.0 | 1.2 | 1.0 | 1.1 | 1.2 | 1.0 | 1.2 | 1.0 | 1.1 | 1.0 | 1.4 | 1.1 | 1.3 | 1.3 | 1.4 |
Intensity Change (Log2) | – | – | – | -0.11 | 0.0 | -0.25 | -0.35 | -0.29 | -0.39 | -0.41 | -0.42 | -0.46 | 0.42 | 0.38 | 0.39 | 0.27 | 0.51 | 0.71 | 0.37 | 0.37 | 0.52 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0.1 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 4.4 | 3.2 | 1.8 | 2.1 | 2.1 | 1.3 | 1.7 | 2.0 | 1.7 | 5.1 | 5.8 | 4.5 | 2.1 | 1.8 | 1.4 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 2.5 | 1.4 | 0 | 0 | 0 | 0 | 0 | 0 | 2.1 | 1.9 | 2.8 | 3.6 | 4.2 | 2.4 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2.9 |
Mitochondria | -2.3 | -3.5 | -0.1 | 0 | 0 | 0 | 0 | 0 | 0 | -9.6 | 0 | 0 | -6.9 | -7.0 | -5.9 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2.1 | 1.6 | 0 |
Vacuole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 3.1235 | 3.2517 | 2.9291 | 1.8638 | 2.2978 | 2.8446 | 2.1094 | 2.9468 | 2.3988 | 1.832 | 1.6282 | 2.2448 | 3.8212 | 4.8622 | 4.1459 | 4.1092 | 3.6625 | 3.9389 |
Actin | 0.1892 | 0.0255 | 0.0326 | 0.0006 | 0.0057 | 0.0155 | 0.0309 | 0.0249 | 0.0054 | 0.0062 | 0.0011 | 0.0038 | 0.0691 | 0.0137 | 0.0146 | 0.0222 | 0.0063 | 0.0149 |
Bud | 0.0004 | 0.001 | 0.0005 | 0.0006 | 0.0004 | 0.0005 | 0.0004 | 0.0021 | 0.0004 | 0.0001 | 0.0002 | 0.0002 | 0.0006 | 0.001 | 0.0005 | 0.0027 | 0.0003 | 0.0003 |
Bud Neck | 0.0007 | 0.0024 | 0.0005 | 0.001 | 0.0014 | 0.009 | 0.0007 | 0.0018 | 0.0005 | 0.0004 | 0.0005 | 0.002 | 0.0008 | 0.0007 | 0.0009 | 0.0012 | 0.0004 | 0.0017 |
Bud Periphery | 0.0003 | 0.0005 | 0.0007 | 0.0004 | 0.0007 | 0.0003 | 0.0004 | 0.0022 | 0.0003 | 0 | 0.0001 | 0.0001 | 0.0006 | 0.0003 | 0.0002 | 0.0014 | 0.001 | 0.0001 |
Bud Site | 0.0013 | 0.0084 | 0.0017 | 0.0027 | 0.0042 | 0.0007 | 0.0011 | 0.0494 | 0.0044 | 0.0003 | 0.0005 | 0.0008 | 0.0029 | 0.0091 | 0.003 | 0.001 | 0.0004 | 0.0004 |
Cell Periphery | 0.0001 | 0.0003 | 0.0001 | 0.0001 | 0.0003 | 0.0001 | 0.0001 | 0.0006 | 0.0003 | 0 | 0 | 0 | 0.0001 | 0.0003 | 0.0001 | 0.0002 | 0.0002 | 0.0001 |
Cytoplasm | 0.1148 | 0.0978 | 0.1734 | 0.1009 | 0.007 | 0.1419 | 0.0928 | 0.0992 | 0.0705 | 0.1116 | 0.0327 | 0.1232 | 0.0459 | 0.1382 | 0.0949 | 0.0799 | 0.0055 | 0.1139 |
Cytoplasmic Foci | 0.1779 | 0.2864 | 0.1863 | 0.2785 | 0.2999 | 0.2822 | 0.2792 | 0.337 | 0.3275 | 0.3249 | 0.3732 | 0.3492 | 0.2954 | 0.3216 | 0.3124 | 0.2701 | 0.3717 | 0.3119 |
Eisosomes | 0.0002 | 0.0001 | 0.0004 | 0 | 0.0002 | 0 | 0.0003 | 0.0001 | 0.0004 | 0 | 0 | 0 | 0.0003 | 0.0002 | 0 | 0.0001 | 0 | 0 |
Endoplasmic Reticulum | 0.0037 | 0.0057 | 0.0087 | 0.0034 | 0.0005 | 0.0043 | 0.0083 | 0.0019 | 0.0016 | 0.0006 | 0.0005 | 0.0027 | 0.0062 | 0.004 | 0.0024 | 0.0016 | 0.0011 | 0.0024 |
Endosome | 0.1989 | 0.2581 | 0.3504 | 0.3119 | 0.2838 | 0.2258 | 0.319 | 0.2278 | 0.2884 | 0.2921 | 0.399 | 0.3313 | 0.3085 | 0.2712 | 0.3237 | 0.3296 | 0.3386 | 0.2792 |
Golgi | 0.1471 | 0.1628 | 0.1147 | 0.1018 | 0.0666 | 0.1596 | 0.0882 | 0.1745 | 0.1401 | 0.119 | 0.1504 | 0.1413 | 0.1071 | 0.1568 | 0.1693 | 0.137 | 0.174 | 0.1695 |
Lipid Particles | 0.01 | 0.0153 | 0.0142 | 0.0114 | 0.0545 | 0.0222 | 0.0152 | 0.0046 | 0.0253 | 0.0036 | 0.0157 | 0.0037 | 0.0237 | 0.0098 | 0.0069 | 0.0062 | 0.0154 | 0.0138 |
Mitochondria | 0.0048 | 0.0052 | 0.0102 | 0.0027 | 0.0457 | 0.0142 | 0.0084 | 0.0052 | 0.007 | 0.001 | 0.003 | 0.0037 | 0.0099 | 0.0061 | 0.007 | 0.0027 | 0.0239 | 0.0113 |
None | 0.0501 | 0.0572 | 0.0085 | 0.0038 | 0.0003 | 0.0192 | 0.0942 | 0.0095 | 0.0527 | 0.0044 | 0.0021 | 0.0018 | 0.0361 | 0.0181 | 0.0101 | 0.064 | 0.0006 | 0.0119 |
Nuclear Periphery | 0.0179 | 0.0104 | 0.004 | 0.0074 | 0.001 | 0.0072 | 0.0033 | 0.0003 | 0.0004 | 0.0001 | 0.0001 | 0.0003 | 0.0081 | 0.0008 | 0.0002 | 0.0003 | 0.0003 | 0.0002 |
Nucleolus | 0.0001 | 0.0003 | 0.0002 | 0.0023 | 0.0017 | 0.0005 | 0.0011 | 0.0001 | 0.0002 | 0 | 0.0001 | 0 | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0 |
Nucleus | 0.0532 | 0.0218 | 0.0134 | 0.13 | 0.1988 | 0.0143 | 0.0024 | 0.0004 | 0.0003 | 0.0001 | 0.0001 | 0.0002 | 0.0015 | 0.0002 | 0.0001 | 0.0011 | 0.0001 | 0.0002 |
Peroxisomes | 0.0244 | 0.0309 | 0.0273 | 0.0219 | 0.0202 | 0.071 | 0.042 | 0.0521 | 0.0691 | 0.1337 | 0.0179 | 0.031 | 0.0749 | 0.042 | 0.05 | 0.0728 | 0.0559 | 0.0637 |
Punctate Nuclear | 0.0021 | 0.0036 | 0.0432 | 0.0045 | 0.0025 | 0.0041 | 0.0069 | 0.0004 | 0.0013 | 0.0004 | 0.0008 | 0.0004 | 0.0035 | 0.001 | 0.0006 | 0.0005 | 0.0005 | 0.0011 |
Vacuole | 0.0018 | 0.0053 | 0.0064 | 0.0115 | 0.003 | 0.0047 | 0.0037 | 0.0046 | 0.0031 | 0.0011 | 0.0012 | 0.0029 | 0.0028 | 0.003 | 0.0018 | 0.0041 | 0.0025 | 0.0023 |
Vacuole Periphery | 0.001 | 0.0011 | 0.0025 | 0.0026 | 0.0017 | 0.0025 | 0.0015 | 0.001 | 0.001 | 0.0005 | 0.0008 | 0.0013 | 0.0015 | 0.0016 | 0.001 | 0.0014 | 0.0013 | 0.001 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 12.821 | 19.3407 | 27.1087 | 40.6747 | 28.0512 | 25.6277 | 24.8064 | 31.0411 | 41.3372 | 34.976 |
Translational Efficiency | 0.7351 | 0.5422 | 0.7379 | 0.529 | 0.4084 | 0.4714 | 0.5217 | 0.5817 | 0.4573 | 0.4201 |
Dataset | Hit |
---|---|
Protein Concentration | ✔ |
Protein Localization | ✘ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
306 | 1312 | 1604 | 1031 | 1542 | 1034 | 1871 | 130 | 1848 | 2346 | 3475 | 1161 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 798.68 | 930.56 | 948.63 | 968.45 | 912.91 | 863.29 | 929.42 | 967.04 | 894.00 | 900.91 | 938.29 | 968.29 |
Standard Deviation | 110.38 | 140.30 | 112.79 | 123.58 | 125.03 | 118.33 | 103.74 | 139.21 | 129.86 | 135.26 | 108.43 | 125.43 |
Intensity Change Log 2 | — | 0.220482 | 0.248228 | 0.278060 | — | -0.080627 | 0.025858 | 0.083103 | — | 0.067722 | 0.133898 | 0.177362 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.002004 | 0.001242 | 0.009742 | 0.012471 | 0.001783 | 0.002284 | 0.003440 | 0.007551 | 0.001819 | 0.001701 | 0.006349 | 0.011920 |
Bud Neck | 0.004061 | 0.001200 | 0.002580 | 0.007504 | 0.001505 | 0.002358 | 0.008159 | 0.008708 | 0.001928 | 0.001710 | 0.005584 | 0.007639 |
Bud Site | 0.020027 | 0.011029 | 0.030620 | 0.063535 | 0.014771 | 0.015157 | 0.013903 | 0.087445 | 0.015641 | 0.012848 | 0.021619 | 0.066212 |
Cell Periphery | 0.000105 | 0.000043 | 0.000054 | 0.000115 | 0.000077 | 0.000129 | 0.000039 | 0.001020 | 0.000082 | 0.000081 | 0.000046 | 0.000216 |
Cytoplasm | 0.027801 | 0.026798 | 0.078184 | 0.135625* | 0.049911 | 0.029450 | 0.049491 | 0.138964* | 0.046250 | 0.027967 | 0.062736 | 0.135999* |
Cytoplasmic Foci | 0.431080* | 0.449053* | 0.437451* | 0.324146* | 0.453360* | 0.409734* | 0.347757* | 0.266613* | 0.449671* | 0.431723* | 0.389158* | 0.317704* |
Eisosomes | 0.000085 | 0.000032 | 0.000043 | 0.000068 | 0.000058 | 0.000054 | 0.000053 | 0.000069 | 0.000063 | 0.000042 | 0.000048 | 0.000068 |
Endoplasmic Reticulum | 0.002808 | 0.000326 | 0.000915 | 0.000705 | 0.001203 | 0.000664 | 0.000699 | 0.000791 | 0.001469 | 0.000475 | 0.000799 | 0.000715 |
Endosome | 0.103655 | 0.135231 | 0.131178 | 0.089970 | 0.111467 | 0.170814 | 0.316398* | 0.111619 | 0.110174 | 0.150915 | 0.230903* | 0.092394 |
Golgi | 0.316224* | 0.286257* | 0.107045 | 0.023661 | 0.292465* | 0.299272* | 0.052873 | 0.025392 | 0.296399* | 0.291994* | 0.077878 | 0.023855 |
Lipid Particles | 0.009072 | 0.003046 | 0.010039 | 0.006469 | 0.006134 | 0.004549 | 0.014588 | 0.005806 | 0.006621 | 0.003708 | 0.012488 | 0.006395 |
Mitochondria | 0.014478 | 0.007663 | 0.001497 | 0.003277 | 0.008490 | 0.009350 | 0.003380 | 0.001947 | 0.009481 | 0.008407 | 0.002511 | 0.003129 |
Mitotic Spindle | 0.021158 | 0.043258 | 0.109334* | 0.080523 | 0.028972 | 0.020347 | 0.047489 | 0.050194 | 0.027678 | 0.033160 | 0.076036 | 0.077127 |
None | 0.000142 | 0.000509 | 0.000045 | 0.000110 | 0.000204 | 0.000058 | 0.000058 | 0.000568 | 0.000194 | 0.000310 | 0.000052 | 0.000162 |
Nuclear Periphery | 0.000070 | 0.000047 | 0.000146 | 0.000194 | 0.000101 | 0.000023 | 0.000431 | 0.000234 | 0.000096 | 0.000037 | 0.000299 | 0.000199 |
Nuclear Periphery Foci | 0.000861 | 0.000618 | 0.003123 | 0.001166 | 0.001128 | 0.000257 | 0.001821 | 0.001188 | 0.001084 | 0.000459 | 0.002422 | 0.001168 |
Nucleolus | 0.000216 | 0.000364 | 0.000085 | 0.000381 | 0.000180 | 0.000102 | 0.001174 | 0.000305 | 0.000186 | 0.000249 | 0.000672 | 0.000372 |
Nucleus | 0.002024 | 0.000825 | 0.001299 | 0.004350 | 0.000523 | 0.000243 | 0.003553 | 0.013703 | 0.000771 | 0.000568 | 0.002513 | 0.005397 |
Peroxisomes | 0.036362 | 0.018011 | 0.039555 | 0.131239* | 0.010293 | 0.022280 | 0.041145 | 0.118518* | 0.014610 | 0.019893 | 0.040411 | 0.129815* |
Vacuole | 0.004459 | 0.009844 | 0.035443 | 0.112644 | 0.010273 | 0.008158 | 0.091200 | 0.157233 | 0.009311 | 0.009101 | 0.065464 | 0.117637 |
Vacuole Periphery | 0.003306 | 0.004604 | 0.001622 | 0.001847 | 0.007103 | 0.004715 | 0.002348 | 0.002132 | 0.006474 | 0.004653 | 0.002013 | 0.001879 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | 1.40 | -7.55 | -5.05 | -5.88 | 0.30 | -0.93 | -2.67 | -2.36 | -2.00 | -1.34 | 0.34 | -8.02 | -5.69 | -5.80 | -1.94 |
Bud Neck | 3.93 | 1.63 | -4.65 | -14.00 | -10.24 | -2.29 | -10.81 | -5.88 | -4.91 | 0.15 | 0.89 | -10.11 | -13.59 | -13.69 | -3.96 |
Bud Site | 2.21 | -2.66 | -6.98 | -13.05 | -6.52 | -0.19 | 0.35 | -5.62 | -5.57 | -5.67 | 1.79 | -4.15 | -12.54 | -13.70 | -10.93 |
Cell Periphery | 2.57 | 2.08 | 0.26 | -2.15 | -1.67 | -0.68 | 3.41 | -4.22 | -3.90 | -4.35 | 0.02 | 3.49 | -3.60 | -2.85 | -4.56 |
Cytoplasm | 0.17 | -9.35 | -16.87 | -19.44 | -9.84 | 4.88 | -0.89 | -6.10 | -7.18 | -5.92 | 5.55 | -6.07 | -17.25 | -20.88 | -14.18 |
Cytoplasmic Foci | -1.56 | 0.52 | 13.07 | 21.42 | 18.16 | 5.80 | 16.42 | 13.09 | 10.48 | 6.78 | 3.09 | 12.23 | 24.35 | 22.92 | 15.97 |
Eisosomes | 4.73 | 3.82 | 2.17 | -5.00 | -3.40 | 0.41 | 0.51 | -0.72 | -1.58 | -1.79 | 2.31 | 1.57 | 0.16 | -3.97 | -2.76 |
Endoplasmic Reticulum | 1.32 | 0.95 | 1.10 | -2.97 | 1.36 | 1.60 | 1.69 | 1.26 | -0.34 | -0.21 | 2.51 | 1.54 | 1.80 | -1.82 | 0.70 |
Endosome | -3.70 | -2.73 | 3.93 | 12.64 | 11.63 | -9.26 | -29.13* | 2.29 | 6.61 | 16.56 | -9.11 | -23.11 | 8.44 | 17.07 | 30.06* |
Golgi | 2.04 | 15.50 | 22.19 | 43.04* | 22.13* | -0.75 | 38.82* | 35.76* | 32.40 | 6.08 | 0.64 | 38.85 | 50.30* | 56.62* | 22.37* |
Lipid Particles | 2.73 | -0.72 | 1.21 | -3.77 | 4.54 | 1.55 | -5.69 | 0.75 | -0.24 | 4.83 | 3.75 | -6.14 | 0.49 | -3.18 | 6.53 |
Mitochondria | 2.19 | 4.48 | 4.06 | 3.88 | -0.94 | -0.49 | 5.34 | 5.81 | 4.62 | 2.07 | 0.84 | 7.61 | 6.27 | 5.32 | 0.36 |
Mitotic Spindle | -4.75 | -14.79 | -9.38 | -6.14 | 3.28 | 2.33 | -5.73 | -2.33 | -2.86 | -0.81 | -1.84 | -14.27 | -9.30 | -8.26 | -0.54 |
None | -1.14 | 3.10 | -1.33 | 0.82 | -2.76 | 4.47 | 3.77 | -2.56 | -3.41 | -3.30 | -0.64 | 4.69 | -1.37 | 0.12 | -3.48 |
Nuclear Periphery | 0.82 | -2.60 | -6.71 | -10.67 | -5.65 | 2.20 | -3.24 | -4.37 | -6.30 | 0.69 | 1.95 | -3.67 | -7.54 | -12.91 | -0.58 |
Nuclear Periphery Foci | 0.31 | -3.84 | -3.30 | -3.59 | 1.96 | 3.63 | -2.49 | -1.74 | -4.72 | 0.68 | 2.06 | -4.34 | -3.44 | -5.31 | 1.41 |
Nucleolus | -0.60 | 1.49 | -2.52 | -0.94 | -3.66 | 1.16 | -3.66 | -1.86 | -2.73 | 3.00 | -0.50 | -3.37 | -3.10 | -1.90 | 0.98 |
Nucleus | 1.06 | 0.34 | -2.98 | -5.30 | -4.72 | 3.29 | -5.33 | -4.01 | -4.05 | -3.47 | 0.74 | -4.90 | -6.73 | -6.90 | -5.16 |
Peroxisomes | 4.19 | -0.52 | -9.39 | -14.94 | -10.62 | -5.78 | -13.97 | -5.12 | -4.23 | -2.92 | -3.32 | -13.90 | -16.58 | -15.41 | -11.22 |
Vacuole | -4.39 | -15.90 | -24.70 | -24.05 | -18.90 | 1.89 | -22.58 | -8.91 | -9.00 | -5.02 | 0.26 | -26.05 | -25.75 | -25.80 | -16.13 |
Vacuole Periphery | -1.75 | 2.66 | 2.76 | 6.67 | 0.72 | 2.82 | 8.03 | 7.61 | 4.08 | 1.29 | 3.02 | 8.92 | 8.88 | 7.33 | 1.87 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
---|
Gene | Images |
---|