Standard name | |
---|---|
Human Ortholog | |
Description | GTPase-activating protein (GAP) for yeast Rab family member Ypt6p; involved in vesicle mediated protein transport |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 10 | 4 | 7 | 2 | 2 | 3 | 0 | 5 | 5 | 4 | 3 | 1 | 8 | 22 | 14 | 24 | 8 | 14 | 21 | 7 | 6 | 24 | 37 | 56 |
Bud | 15 | 4 | 2 | 2 | 4 | 3 | 8 | 12 | 9 | 9 | 6 | 11 | 3 | 13 | 1 | 4 | 3 | 2 | 2 | 3 | 0 | 3 | 2 | 8 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 3 | 1 | 0 | 2 | 6 | 8 |
Bud Site | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 1 | 1 | 2 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 2 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 2 | 1 | 1 | 3 | 1 | 4 | 1 | 3 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 |
Cytoplasm | 78 | 20 | 5 | 35 | 23 | 26 | 51 | 60 | 49 | 53 | 41 | 51 | 113 | 232 | 189 | 64 | 41 | 18 | 26 | 13 | 6 | 63 | 122 | 113 |
Endoplasmic Reticulum | 5 | 4 | 2 | 2 | 0 | 4 | 4 | 12 | 19 | 10 | 5 | 9 | 22 | 25 | 24 | 11 | 4 | 5 | 9 | 1 | 2 | 11 | 27 | 64 |
Endosome | 220 | 17 | 3 | 3 | 15 | 16 | 16 | 26 | 7 | 6 | 4 | 7 | 44 | 77 | 107 | 82 | 68 | 20 | 199 | 40 | 32 | 87 | 129 | 131 |
Golgi | 7 | 12 | 1 | 0 | 0 | 2 | 1 | 0 | 2 | 1 | 0 | 1 | 5 | 14 | 29 | 12 | 13 | 18 | 132 | 20 | 10 | 34 | 57 | 86 |
Mitochondria | 146 | 12 | 25 | 25 | 14 | 61 | 99 | 115 | 169 | 153 | 113 | 161 | 9 | 21 | 17 | 32 | 18 | 8 | 35 | 4 | 11 | 30 | 34 | 48 |
Nucleus | 5 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 1 | 1 | 2 |
Nucleolus | 7 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 2 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 |
Peroxisomes | 16 | 0 | 0 | 0 | 0 | 2 | 2 | 8 | 0 | 2 | 0 | 2 | 0 | 3 | 1 | 0 | 2 | 0 | 11 | 1 | 3 | 2 | 2 | 4 |
SpindlePole | 9 | 0 | 2 | 0 | 4 | 1 | 6 | 14 | 5 | 7 | 0 | 1 | 0 | 5 | 6 | 42 | 23 | 5 | 12 | 3 | 4 | 7 | 10 | 13 |
Vac/Vac Membrane | 11 | 3 | 1 | 1 | 1 | 1 | 5 | 7 | 1 | 7 | 1 | 4 | 3 | 10 | 16 | 8 | 13 | 4 | 10 | 7 | 2 | 10 | 19 | 32 |
Unique Cell Count | 415 | 58 | 39 | 61 | 52 | 96 | 172 | 206 | 222 | 207 | 142 | 204 | 196 | 381 | 372 | 223 | 154 | 74 | 475 | 107 | 84 | 285 | 461 | 585 |
Labelled Cell Count | 531 | 78 | 49 | 71 | 63 | 120 | 195 | 262 | 269 | 257 | 174 | 253 | 208 | 428 | 406 | 283 | 195 | 96 | 475 | 107 | 84 | 285 | 461 | 585 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 7.0 | 7.8 | 5.9 | 5.5 | 6.0 | 5.0 | 4.7 | 4.9 | 4.5 | 4.5 | 4.4 | 4.3 | 7.9 | 7.7 | 7.8 | 7.1 | 8.5 | 9.7 | 7.7 | 7.7 | 8.5 |
Std Deviation (1e-4) | 0.9 | 1.1 | 0.8 | 0.7 | 1.3 | 0.7 | 1.0 | 1.2 | 1.0 | 1.1 | 1.2 | 1.0 | 1.2 | 1.0 | 1.1 | 1.0 | 1.4 | 1.1 | 1.3 | 1.3 | 1.4 |
Intensity Change (Log2) | – | – | – | -0.11 | 0.0 | -0.25 | -0.35 | -0.29 | -0.39 | -0.41 | -0.42 | -0.46 | 0.42 | 0.38 | 0.39 | 0.27 | 0.51 | 0.71 | 0.37 | 0.37 | 0.52 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0.1 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 4.4 | 3.2 | 1.8 | 2.1 | 2.1 | 1.3 | 1.7 | 2.0 | 1.7 | 5.1 | 5.8 | 4.5 | 2.1 | 1.8 | 1.4 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 2.5 | 1.4 | 0 | 0 | 0 | 0 | 0 | 0 | 2.1 | 1.9 | 2.8 | 3.6 | 4.2 | 2.4 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2.9 |
Mitochondria | -2.3 | -3.5 | -0.1 | 0 | 0 | 0 | 0 | 0 | 0 | -9.6 | 0 | 0 | -6.9 | -7.0 | -5.9 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2.1 | 1.6 | 0 |
Vacuole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 3.1235 | 3.2517 | 2.9291 | 1.8638 | 2.2978 | 2.8446 | 2.1094 | 2.9468 | 2.3988 | 1.832 | 1.6282 | 2.2448 | 3.8212 | 4.8622 | 4.1459 | 4.1092 | 3.6625 | 3.9389 |
Actin | 0.1892 | 0.0255 | 0.0326 | 0.0006 | 0.0057 | 0.0155 | 0.0309 | 0.0249 | 0.0054 | 0.0062 | 0.0011 | 0.0038 | 0.0691 | 0.0137 | 0.0146 | 0.0222 | 0.0063 | 0.0149 |
Bud | 0.0004 | 0.001 | 0.0005 | 0.0006 | 0.0004 | 0.0005 | 0.0004 | 0.0021 | 0.0004 | 0.0001 | 0.0002 | 0.0002 | 0.0006 | 0.001 | 0.0005 | 0.0027 | 0.0003 | 0.0003 |
Bud Neck | 0.0007 | 0.0024 | 0.0005 | 0.001 | 0.0014 | 0.009 | 0.0007 | 0.0018 | 0.0005 | 0.0004 | 0.0005 | 0.002 | 0.0008 | 0.0007 | 0.0009 | 0.0012 | 0.0004 | 0.0017 |
Bud Periphery | 0.0003 | 0.0005 | 0.0007 | 0.0004 | 0.0007 | 0.0003 | 0.0004 | 0.0022 | 0.0003 | 0 | 0.0001 | 0.0001 | 0.0006 | 0.0003 | 0.0002 | 0.0014 | 0.001 | 0.0001 |
Bud Site | 0.0013 | 0.0084 | 0.0017 | 0.0027 | 0.0042 | 0.0007 | 0.0011 | 0.0494 | 0.0044 | 0.0003 | 0.0005 | 0.0008 | 0.0029 | 0.0091 | 0.003 | 0.001 | 0.0004 | 0.0004 |
Cell Periphery | 0.0001 | 0.0003 | 0.0001 | 0.0001 | 0.0003 | 0.0001 | 0.0001 | 0.0006 | 0.0003 | 0 | 0 | 0 | 0.0001 | 0.0003 | 0.0001 | 0.0002 | 0.0002 | 0.0001 |
Cytoplasm | 0.1148 | 0.0978 | 0.1734 | 0.1009 | 0.007 | 0.1419 | 0.0928 | 0.0992 | 0.0705 | 0.1116 | 0.0327 | 0.1232 | 0.0459 | 0.1382 | 0.0949 | 0.0799 | 0.0055 | 0.1139 |
Cytoplasmic Foci | 0.1779 | 0.2864 | 0.1863 | 0.2785 | 0.2999 | 0.2822 | 0.2792 | 0.337 | 0.3275 | 0.3249 | 0.3732 | 0.3492 | 0.2954 | 0.3216 | 0.3124 | 0.2701 | 0.3717 | 0.3119 |
Eisosomes | 0.0002 | 0.0001 | 0.0004 | 0 | 0.0002 | 0 | 0.0003 | 0.0001 | 0.0004 | 0 | 0 | 0 | 0.0003 | 0.0002 | 0 | 0.0001 | 0 | 0 |
Endoplasmic Reticulum | 0.0037 | 0.0057 | 0.0087 | 0.0034 | 0.0005 | 0.0043 | 0.0083 | 0.0019 | 0.0016 | 0.0006 | 0.0005 | 0.0027 | 0.0062 | 0.004 | 0.0024 | 0.0016 | 0.0011 | 0.0024 |
Endosome | 0.1989 | 0.2581 | 0.3504 | 0.3119 | 0.2838 | 0.2258 | 0.319 | 0.2278 | 0.2884 | 0.2921 | 0.399 | 0.3313 | 0.3085 | 0.2712 | 0.3237 | 0.3296 | 0.3386 | 0.2792 |
Golgi | 0.1471 | 0.1628 | 0.1147 | 0.1018 | 0.0666 | 0.1596 | 0.0882 | 0.1745 | 0.1401 | 0.119 | 0.1504 | 0.1413 | 0.1071 | 0.1568 | 0.1693 | 0.137 | 0.174 | 0.1695 |
Lipid Particles | 0.01 | 0.0153 | 0.0142 | 0.0114 | 0.0545 | 0.0222 | 0.0152 | 0.0046 | 0.0253 | 0.0036 | 0.0157 | 0.0037 | 0.0237 | 0.0098 | 0.0069 | 0.0062 | 0.0154 | 0.0138 |
Mitochondria | 0.0048 | 0.0052 | 0.0102 | 0.0027 | 0.0457 | 0.0142 | 0.0084 | 0.0052 | 0.007 | 0.001 | 0.003 | 0.0037 | 0.0099 | 0.0061 | 0.007 | 0.0027 | 0.0239 | 0.0113 |
None | 0.0501 | 0.0572 | 0.0085 | 0.0038 | 0.0003 | 0.0192 | 0.0942 | 0.0095 | 0.0527 | 0.0044 | 0.0021 | 0.0018 | 0.0361 | 0.0181 | 0.0101 | 0.064 | 0.0006 | 0.0119 |
Nuclear Periphery | 0.0179 | 0.0104 | 0.004 | 0.0074 | 0.001 | 0.0072 | 0.0033 | 0.0003 | 0.0004 | 0.0001 | 0.0001 | 0.0003 | 0.0081 | 0.0008 | 0.0002 | 0.0003 | 0.0003 | 0.0002 |
Nucleolus | 0.0001 | 0.0003 | 0.0002 | 0.0023 | 0.0017 | 0.0005 | 0.0011 | 0.0001 | 0.0002 | 0 | 0.0001 | 0 | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0 |
Nucleus | 0.0532 | 0.0218 | 0.0134 | 0.13 | 0.1988 | 0.0143 | 0.0024 | 0.0004 | 0.0003 | 0.0001 | 0.0001 | 0.0002 | 0.0015 | 0.0002 | 0.0001 | 0.0011 | 0.0001 | 0.0002 |
Peroxisomes | 0.0244 | 0.0309 | 0.0273 | 0.0219 | 0.0202 | 0.071 | 0.042 | 0.0521 | 0.0691 | 0.1337 | 0.0179 | 0.031 | 0.0749 | 0.042 | 0.05 | 0.0728 | 0.0559 | 0.0637 |
Punctate Nuclear | 0.0021 | 0.0036 | 0.0432 | 0.0045 | 0.0025 | 0.0041 | 0.0069 | 0.0004 | 0.0013 | 0.0004 | 0.0008 | 0.0004 | 0.0035 | 0.001 | 0.0006 | 0.0005 | 0.0005 | 0.0011 |
Vacuole | 0.0018 | 0.0053 | 0.0064 | 0.0115 | 0.003 | 0.0047 | 0.0037 | 0.0046 | 0.0031 | 0.0011 | 0.0012 | 0.0029 | 0.0028 | 0.003 | 0.0018 | 0.0041 | 0.0025 | 0.0023 |
Vacuole Periphery | 0.001 | 0.0011 | 0.0025 | 0.0026 | 0.0017 | 0.0025 | 0.0015 | 0.001 | 0.001 | 0.0005 | 0.0008 | 0.0013 | 0.0015 | 0.0016 | 0.001 | 0.0014 | 0.0013 | 0.001 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 12.821 | 19.3407 | 27.1087 | 40.6747 | 28.0512 | 25.6277 | 24.8064 | 31.0411 | 41.3372 | 34.976 |
Translational Efficiency | 0.7351 | 0.5422 | 0.7379 | 0.529 | 0.4084 | 0.4714 | 0.5217 | 0.5817 | 0.4573 | 0.4201 |
Dataset | Hit |
---|---|
Protein Concentration | ✔ |
Protein Localization | ✘ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
---|
Gene | Images |
---|