Standard name | |
---|---|
Human Ortholog | |
Description | Kynurenine aminotransferase; catalyzes formation of kynurenic acid from kynurenine; potential Cdc28p substrate |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 8 | 8 | 40 | 17 | 15 | 10 | 20 | 16 | 10 | 17 | 7 | 16 | 10 | 23 | 14 | 7 | 11 | 4 | 7 | 6 | 25 | 6 | 16 | 20 |
Bud | 2 | 1 | 9 | 6 | 5 | 14 | 9 | 8 | 7 | 21 | 14 | 23 | 1 | 0 | 1 | 2 | 2 | 1 | 2 | 2 | 6 | 7 | 17 | 20 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 3 | 1 | 1 | 8 | 11 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 1 | 2 | 2 | 5 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 2 | 2 | 18 | 6 | 5 | 10 | 5 | 14 | 11 | 9 | 8 | 18 | 7 | 24 | 22 | 18 | 21 | 11 | 0 | 1 | 7 | 1 | 1 | 2 |
Cytoplasm | 107 | 91 | 133 | 48 | 82 | 52 | 63 | 50 | 41 | 48 | 39 | 43 | 109 | 357 | 355 | 241 | 290 | 208 | 70 | 63 | 77 | 84 | 132 | 107 |
Endoplasmic Reticulum | 7 | 8 | 35 | 9 | 22 | 16 | 25 | 30 | 47 | 33 | 19 | 27 | 4 | 20 | 16 | 9 | 7 | 11 | 12 | 3 | 25 | 4 | 10 | 15 |
Endosome | 17 | 7 | 27 | 32 | 25 | 34 | 27 | 68 | 20 | 30 | 5 | 27 | 19 | 47 | 32 | 4 | 6 | 4 | 41 | 29 | 81 | 37 | 63 | 57 |
Golgi | 5 | 3 | 44 | 29 | 62 | 33 | 19 | 66 | 48 | 30 | 16 | 27 | 1 | 4 | 3 | 19 | 22 | 10 | 9 | 8 | 52 | 9 | 17 | 16 |
Mitochondria | 21 | 21 | 33 | 86 | 93 | 200 | 347 | 277 | 271 | 480 | 354 | 428 | 1 | 1 | 2 | 3 | 8 | 5 | 27 | 32 | 94 | 41 | 70 | 61 |
Nucleus | 0 | 0 | 0 | 0 | 3 | 0 | 7 | 3 | 3 | 4 | 0 | 4 | 0 | 0 | 1 | 0 | 3 | 3 | 0 | 2 | 0 | 2 | 4 | 4 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 3 | 9 | 15 | 9 | 5 | 5 | 10 | 0 | 0 | 0 | 0 | 0 | 0 | 4 | 0 | 4 | 1 | 3 | 4 |
Nucleolus | 0 | 0 | 1 | 1 | 2 | 10 | 28 | 36 | 15 | 36 | 8 | 24 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 2 | 1 |
Peroxisomes | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 |
SpindlePole | 1 | 1 | 2 | 0 | 0 | 0 | 4 | 9 | 2 | 1 | 2 | 4 | 0 | 0 | 0 | 0 | 1 | 1 | 5 | 2 | 9 | 5 | 12 | 11 |
Vac/Vac Membrane | 10 | 6 | 27 | 12 | 35 | 101 | 125 | 194 | 94 | 160 | 93 | 149 | 18 | 60 | 40 | 43 | 40 | 66 | 8 | 15 | 13 | 29 | 38 | 43 |
Unique Cell Count | 164 | 133 | 295 | 188 | 264 | 383 | 565 | 644 | 481 | 725 | 484 | 655 | 148 | 482 | 441 | 314 | 368 | 282 | 203 | 176 | 419 | 239 | 411 | 390 |
Labelled Cell Count | 180 | 148 | 370 | 247 | 349 | 483 | 690 | 788 | 580 | 876 | 575 | 801 | 170 | 536 | 489 | 346 | 411 | 325 | 203 | 176 | 419 | 239 | 411 | 390 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 8.4 | 7.9 | 10.0 | 9.1 | 10.5 | 10.0 | 9.3 | 9.6 | 8.9 | 8.5 | 8.1 | 8.2 | 9.7 | 9.8 | 9.2 | 17.6 | 17.0 | 17.9 | 10.4 | 10.3 | 10.7 |
Std Deviation (1e-4) | 2.1 | 2.0 | 2.2 | 2.1 | 2.1 | 2.3 | 2.1 | 2.1 | 2.1 | 2.0 | 2.2 | 2.1 | 1.8 | 1.8 | 1.9 | 4.7 | 4.7 | 5.5 | 2.0 | 2.1 | 2.1 |
Intensity Change (Log2) | – | – | – | -0.14 | 0.07 | -0.0 | -0.11 | -0.06 | -0.16 | -0.24 | -0.3 | -0.29 | -0.04 | -0.02 | -0.12 | 0.82 | 0.77 | 0.84 | 0.06 | 0.05 | 0.1 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | -1.5 | -3.1 | -5.4 | -5.5 | -6.7 | -6.3 | -7.1 | -6.9 | -6.7 | -2.1 | -4.4 | -5.3 | -5.2 | -5.1 | -5.5 |
Bud | 0.1 | -0.9 | 0.4 | -1.4 | -1.9 | -1.5 | -0.1 | -0.1 | 0.4 | 0 | 0 | 0 | -2.2 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | -1.4 | -2.5 | -2.3 | -4.5 | -3.1 | -2.7 | -4.4 | -3.4 | -2.5 | -0.6 | -0.7 | -0.7 | -0.2 | -0.2 | -1.2 |
Cytoplasm | -4.3 | -3.4 | -9.1 | -11.3 | -13.4 | -11.9 | -14.6 | -12.1 | -14.1 | 5.7 | 8.1 | 10.0 | 8.0 | 9.0 | 7.0 |
Endoplasmic Reticulum | -2.6 | -1.4 | -3.8 | -4.1 | -4.0 | -0.9 | -4.2 | -4.2 | -4.5 | -3.2 | -4.1 | -4.3 | -4.3 | -5.2 | -3.5 |
Endosome | 2.6 | 0.1 | -0.1 | -2.5 | 0.7 | -2.8 | -3.2 | -5.5 | -3.1 | 1.2 | 0.3 | -0.9 | -4.4 | -4.4 | -4.1 |
Golgi | 0.2 | 2.6 | -2.6 | -6.2 | -2.1 | -2.1 | -6.0 | -5.9 | -5.9 | -4.7 | -7.9 | -7.7 | -3.6 | -3.8 | -4.7 |
Mitochondria | 8.6 | 6.8 | 11.2 | 14.1 | 9.6 | 12.5 | 15.9 | 16.8 | 15.5 | -3.9 | -7.3 | -6.7 | -5.4 | -4.8 | -4.6 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 3.6 | 3.8 | 2.6 | 3.6 | 0 | 3.0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | -1.1 | 1.5 | 5.7 | 4.7 | 7.0 | 3.9 | 4.8 | 3.8 | 5.0 | 1.0 | 1.4 | 0 | 1.8 | 0.7 | 4.7 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 9.5966 | 11.7591 | 10.2194 | 10.1651 | 9.7146 | 9.9991 | 7.54 | 9.605 | 8.7786 | 7.7801 | 8.4739 | 8.6013 | 5.3889 | 7.4733 | 6.6146 | 5.6126 | 6.1513 | 6.4528 |
Actin | 0.0391 | 0.0811 | 0.0885 | 0.0641 | 0.0462 | 0.036 | 0.0337 | 0.019 | 0.0454 | 0.0425 | 0.0226 | 0.0157 | 0.0439 | 0.04 | 0.0315 | 0.0384 | 0.0494 | 0.0266 |
Bud | 0.007 | 0.0063 | 0.0119 | 0.0154 | 0.0118 | 0.0064 | 0.0053 | 0.0117 | 0.0105 | 0.0277 | 0.0134 | 0.004 | 0.0048 | 0.0061 | 0.0074 | 0.0159 | 0.0154 | 0.0071 |
Bud Neck | 0.0024 | 0.0031 | 0.0056 | 0.0039 | 0.0023 | 0.0062 | 0.0032 | 0.0018 | 0.0035 | 0.0026 | 0.0016 | 0.007 | 0.0027 | 0.0018 | 0.0018 | 0.0018 | 0.0018 | 0.0072 |
Bud Periphery | 0.007 | 0.0041 | 0.0123 | 0.0152 | 0.02 | 0.0094 | 0.0048 | 0.0051 | 0.0089 | 0.041 | 0.0149 | 0.0036 | 0.0052 | 0.0028 | 0.0062 | 0.0154 | 0.0128 | 0.0104 |
Bud Site | 0.0142 | 0.0426 | 0.023 | 0.011 | 0.0049 | 0.0101 | 0.0129 | 0.0267 | 0.0132 | 0.0068 | 0.0034 | 0.002 | 0.0149 | 0.0295 | 0.007 | 0.0066 | 0.0076 | 0.0025 |
Cell Periphery | 0.0011 | 0.0014 | 0.0071 | 0.0011 | 0.0009 | 0.0007 | 0.0006 | 0.0008 | 0.0007 | 0.0014 | 0.0014 | 0.0003 | 0.0008 | 0.0006 | 0.0005 | 0.0005 | 0.0003 | 0.0005 |
Cytoplasm | 0.1834 | 0.3019 | 0.2284 | 0.335 | 0.1787 | 0.1248 | 0.237 | 0.3913 | 0.41 | 0.3728 | 0.3035 | 0.2789 | 0.2236 | 0.3675 | 0.3573 | 0.3035 | 0.2915 | 0.2831 |
Cytoplasmic Foci | 0.1208 | 0.1419 | 0.1069 | 0.092 | 0.1224 | 0.113 | 0.1029 | 0.1209 | 0.0948 | 0.0854 | 0.1278 | 0.1104 | 0.1087 | 0.1482 | 0.1309 | 0.0934 | 0.1312 | 0.1199 |
Eisosomes | 0.0003 | 0.0004 | 0.0007 | 0.0002 | 0.0003 | 0.0002 | 0.0002 | 0.0001 | 0.0002 | 0.0002 | 0.0004 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0.0002 | 0.0001 | 0.0001 |
Endoplasmic Reticulum | 0.0475 | 0.0101 | 0.0177 | 0.0121 | 0.0402 | 0.0255 | 0.0282 | 0.0093 | 0.0142 | 0.0071 | 0.0141 | 0.0244 | 0.0269 | 0.0041 | 0.0102 | 0.0155 | 0.0048 | 0.014 |
Endosome | 0.2237 | 0.1526 | 0.1365 | 0.1595 | 0.1791 | 0.2166 | 0.2756 | 0.1963 | 0.1504 | 0.184 | 0.2668 | 0.2726 | 0.2645 | 0.1818 | 0.1964 | 0.1866 | 0.1879 | 0.2453 |
Golgi | 0.1478 | 0.1131 | 0.1364 | 0.1045 | 0.144 | 0.1968 | 0.0997 | 0.1153 | 0.1033 | 0.0665 | 0.0844 | 0.1245 | 0.1252 | 0.1555 | 0.1122 | 0.1328 | 0.1023 | 0.1392 |
Lipid Particles | 0.0116 | 0.0026 | 0.0016 | 0.0012 | 0.0017 | 0.0025 | 0.0097 | 0.0013 | 0.0044 | 0.0023 | 0.0068 | 0.0062 | 0.0068 | 0.0013 | 0.0014 | 0.004 | 0.0045 | 0.0014 |
Mitochondria | 0.1298 | 0.1124 | 0.1854 | 0.1461 | 0.1878 | 0.2053 | 0.0974 | 0.0587 | 0.1002 | 0.1332 | 0.097 | 0.1108 | 0.0681 | 0.0341 | 0.097 | 0.1435 | 0.1427 | 0.1037 |
None | 0.0055 | 0.0013 | 0.0018 | 0.0012 | 0.0024 | 0.0049 | 0.004 | 0.0012 | 0.0017 | 0.0017 | 0.0015 | 0.0019 | 0.0113 | 0.0075 | 0.003 | 0.0011 | 0.0021 | 0.0014 |
Nuclear Periphery | 0.0088 | 0.0028 | 0.0033 | 0.0024 | 0.0023 | 0.0055 | 0.0203 | 0.0034 | 0.0085 | 0.0026 | 0.0026 | 0.0054 | 0.0177 | 0.0008 | 0.0029 | 0.0034 | 0.0077 | 0.0067 |
Nucleolus | 0.0004 | 0.0002 | 0.0002 | 0.0002 | 0.0001 | 0.0002 | 0.0013 | 0.0003 | 0.0004 | 0.0002 | 0.0002 | 0.0002 | 0.0013 | 0.0001 | 0.0001 | 0.0001 | 0.0005 | 0.0001 |
Nucleus | 0.0026 | 0.002 | 0.0015 | 0.0028 | 0.0015 | 0.0021 | 0.0067 | 0.0026 | 0.0033 | 0.002 | 0.0016 | 0.0021 | 0.0109 | 0.001 | 0.0022 | 0.0019 | 0.0027 | 0.0021 |
Peroxisomes | 0.0244 | 0.0093 | 0.0189 | 0.0084 | 0.0354 | 0.0147 | 0.0117 | 0.0121 | 0.0088 | 0.0066 | 0.0161 | 0.0074 | 0.0097 | 0.0095 | 0.011 | 0.0088 | 0.0198 | 0.0112 |
Punctate Nuclear | 0.0015 | 0.0007 | 0.0005 | 0.0005 | 0.0009 | 0.0006 | 0.0045 | 0.0007 | 0.0011 | 0.0008 | 0.0062 | 0.0012 | 0.0127 | 0.0006 | 0.0007 | 0.0005 | 0.0032 | 0.0005 |
Vacuole | 0.0129 | 0.0078 | 0.0076 | 0.0139 | 0.0117 | 0.0111 | 0.0273 | 0.0155 | 0.0112 | 0.0068 | 0.0077 | 0.0118 | 0.0291 | 0.0047 | 0.0141 | 0.0156 | 0.0061 | 0.0094 |
Vacuole Periphery | 0.0082 | 0.0024 | 0.0043 | 0.0094 | 0.0055 | 0.0073 | 0.013 | 0.0061 | 0.0053 | 0.0056 | 0.0061 | 0.0095 | 0.0111 | 0.0025 | 0.0061 | 0.0103 | 0.0058 | 0.0077 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 58.2697 | 26.5989 | 36.1855 | 51.7561 | 36.9709 | 50.3898 | 44.0765 | 42.8582 | 52.9024 | 41.3054 |
Translational Efficiency | 1.1553 | 1.4567 | 1.3017 | 1.3377 | 1.0915 | 1.6777 | 1.1914 | 1.3308 | 1.453 | 1.4664 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✔ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
103 | 240 | 1540 | 1254 | 1145 | 1762 | 379 | 301 | 1248 | 2002 | 1919 | 1555 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 2007.11 | 966.15 | 876.28 | 814.81 | 841.89 | 952.44 | 971.11 | 976.78 | 938.06 | 954.08 | 895.01 | 846.16 |
Standard Deviation | 1468.97 | 200.97 | 129.06 | 159.89 | 132.65 | 159.93 | 128.86 | 203.03 | 545.02 | 165.45 | 134.43 | 180.80 |
Intensity Change Log 2 | — | -1.054801 | -1.195656 | -1.300584 | — | 0.177996 | 0.206003 | 0.214402 | — | -0.570409 | -0.624967 | -0.669215 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.010090 | 0.005108 | 0.002107 | 0.002927 | 0.002161 | 0.001319 | 0.001416 | 0.049352 | 0.002816 | 0.001773 | 0.001971 | 0.011913 |
Bud Neck | 0.010193 | 0.011561 | 0.022947 | 0.020455 | 0.013797 | 0.006195 | 0.018527 | 0.018471 | 0.013500 | 0.006838 | 0.022074 | 0.020071 |
Bud Site | 0.112863* | 0.145442* | 0.051329 | 0.058958 | 0.063555 | 0.045376 | 0.037543 | 0.153434* | 0.067625 | 0.057372 | 0.048606 | 0.077246 |
Cell Periphery | 0.007995 | 0.000616 | 0.000181 | 0.000214 | 0.000580 | 0.000192 | 0.000244 | 0.000772 | 0.001192 | 0.000243 | 0.000194 | 0.000322 |
Cytoplasm | 0.067828 | 0.054841 | 0.259553* | 0.311884* | 0.063255 | 0.042135 | 0.202574* | 0.209086* | 0.063632 | 0.043658 | 0.248300* | 0.291985* |
Cytoplasmic Foci | 0.048446 | 0.179571 | 0.145623 | 0.095625 | 0.208369* | 0.135891 | 0.172149 | 0.122048 | 0.195170* | 0.141127 | 0.150862 | 0.100739 |
Eisosomes | 0.000143 | 0.000097 | 0.000077 | 0.000087 | 0.000141 | 0.000062 | 0.000150 | 0.000260 | 0.000141 | 0.000066 | 0.000091 | 0.000121 |
Endoplasmic Reticulum | 0.008308 | 0.001189 | 0.003522 | 0.002307 | 0.003675 | 0.000685 | 0.004247 | 0.001955 | 0.004057 | 0.000745 | 0.003665 | 0.002239 |
Endosome | 0.032772 | 0.162595 | 0.060393 | 0.040847 | 0.116560 | 0.117939 | 0.092157 | 0.037643 | 0.109645 | 0.123292 | 0.066667 | 0.040227 |
Golgi | 0.217945* | 0.176753* | 0.029461 | 0.025606 | 0.161556* | 0.197433* | 0.021840 | 0.046207 | 0.166210* | 0.194954* | 0.027956 | 0.029594 |
Lipid Particles | 0.003338 | 0.002241 | 0.005296 | 0.003348 | 0.005563 | 0.001395 | 0.009864 | 0.001548 | 0.005379 | 0.001496 | 0.006198 | 0.002999 |
Mitochondria | 0.107331* | 0.106415* | 0.039499 | 0.020495 | 0.212955* | 0.281101* | 0.007971 | 0.016170 | 0.204238* | 0.260160* | 0.033273 | 0.019658 |
Mitotic Spindle | 0.032092 | 0.048309 | 0.069447 | 0.048866 | 0.028487 | 0.047328 | 0.029009 | 0.037263 | 0.028785 | 0.047446 | 0.061460 | 0.046620 |
None | 0.010933 | 0.000308 | 0.002040 | 0.003988 | 0.000756 | 0.000746 | 0.002277 | 0.006407 | 0.001596 | 0.000694 | 0.002087 | 0.004456 |
Nuclear Periphery | 0.000414 | 0.000082 | 0.001566 | 0.001680 | 0.000366 | 0.000187 | 0.001145 | 0.000409 | 0.000370 | 0.000175 | 0.001483 | 0.001434 |
Nuclear Periphery Foci | 0.011162 | 0.000462 | 0.001827 | 0.001505 | 0.001142 | 0.000348 | 0.002801 | 0.000658 | 0.001969 | 0.000362 | 0.002019 | 0.001341 |
Nucleolus | 0.013473 | 0.000510 | 0.002133 | 0.002324 | 0.000802 | 0.000814 | 0.004764 | 0.000224 | 0.001847 | 0.000777 | 0.002652 | 0.001917 |
Nucleus | 0.219768* | 0.004685 | 0.153573* | 0.128274 | 0.003573 | 0.001709 | 0.152681* | 0.051091 | 0.021416 | 0.002066 | 0.153397* | 0.113334 |
Peroxisomes | 0.009270 | 0.046630 | 0.026020 | 0.019680 | 0.026969 | 0.012491 | 0.040644 | 0.100952* | 0.025509 | 0.016584 | 0.028908 | 0.035411 |
Vacuole | 0.034359 | 0.033998 | 0.112485 | 0.195438 | 0.047279 | 0.062156 | 0.189522 | 0.141671 | 0.046212 | 0.058780 | 0.127700 | 0.185030 |
Vacuole Periphery | 0.041277 | 0.018586 | 0.010920 | 0.015494 | 0.038458 | 0.044499 | 0.008472 | 0.004377 | 0.038691 | 0.041393 | 0.010437 | 0.013342 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | 1.81 | 3.11 | 3.01 | 2.50 | -0.02 | 2.13 | 2.42 | -3.75 | -3.88 | -3.88 | 2.40 | 2.40 | -2.79 | -3.55 | -3.49 |
Bud Neck | -0.13 | -3.17 | -1.73 | -2.71 | 2.57 | 4.67 | -1.18 | -0.47 | -3.58 | 0.50 | 4.41 | -4.19 | -1.82 | -7.48 | 2.59 |
Bud Site | -1.30 | 3.18 | 3.41 | 6.77 | 1.16 | 4.10 | 5.53 | -2.89 | -4.77 | -5.68 | 2.15 | 4.94 | 2.82 | 0.94 | -1.90 |
Cell Periphery | 1.62 | 1.72 | 1.72 | 3.44 | 0.23 | 2.78 | 2.39 | 0.55 | -1.67 | -1.34 | 2.39 | 2.53 | 2.42 | 0.37 | -1.12 |
Cytoplasm | 0.86 | -10.59 | -13.39 | -23.33 | -5.22 | 4.23 | -11.72 | -11.45 | -13.13 | -1.60 | 4.18 | -25.27 | -28.81 | -34.15 | -5.43 |
Cytoplasmic Foci | -9.73 | -9.44 | -3.44 | 9.04 | 10.52 | 11.59 | 4.91 | 12.16 | 4.87 | 5.61 | 9.02 | 8.12 | 18.66 | 12.74 | 11.92 |
Eisosomes | 1.21 | 1.70 | 1.56 | 1.40 | -1.15 | 3.93 | -0.61 | -0.77 | -2.31 | -0.54 | 4.01 | 2.47 | 1.73 | -3.48 | -0.86 |
Endoplasmic Reticulum | 3.63 | 2.33 | 3.01 | -3.24 | 4.00 | 4.34 | -0.75 | 1.51 | -6.54 | 2.24 | 5.06 | 0.25 | 2.37 | -8.57 | 4.57 |
Endosome | -10.90 | -4.71 | 0.49 | 11.74 | 8.05 | -0.24 | 4.63 | 16.97 | 19.72 | 7.75 | -2.73 | 10.57 | 19.19 | 26.94 | 10.78 |
Golgi | 1.12 | 6.46 | 6.77 | 12.18 | 3.04 | -4.66 | 22.03 | 18.10 | 23.77 | -1.08 | -3.90 | 23.77 | 24.35 | 33.52 | 2.27 |
Lipid Particles | 0.86 | -0.87 | 0.49 | -0.85 | 4.72 | 5.21 | -2.03 | 4.60 | -2.00 | 3.98 | 5.21 | -1.02 | 3.46 | -4.50 | 6.13 |
Mitochondria | -0.06 | 3.11 | 4.09 | 7.15 | 6.05 | -6.37 | 25.39 | 24.47 | 36.25* | -0.72 | -5.59 | 21.97 | 24.60 | 36.53* | 5.74 |
Mitotic Spindle | -1.35 | -3.45 | -0.82 | 0.93 | 4.55 | -4.72 | 0.45 | -0.60 | 2.37 | -0.84 | -4.95 | -6.69 | -2.40 | 2.06 | 4.02 |
None | 2.85 | 2.41 | 2.05 | -3.33 | -1.38 | 0.09 | -2.13 | -1.42 | -1.43 | -0.84 | 2.55 | -1.00 | -1.97 | -3.15 | -1.61 |
Nuclear Periphery | 3.45 | -8.27 | -7.79 | -10.51 | -1.81 | 2.10 | -7.44 | -4.10 | -5.49 | 1.35 | 2.52 | -10.52 | -8.90 | -10.75 | -1.51 |
Nuclear Periphery Foci | 2.22 | 1.91 | 2.03 | -3.70 | 1.88 | 1.53 | -3.03 | -0.51 | -4.76 | 3.42 | 2.62 | -0.45 | 0.88 | -4.94 | 3.00 |
Nucleolus | 2.47 | 2.14 | 2.08 | -3.37 | -0.44 | -0.00 | -2.46 | 2.52 | 2.60 | 2.77 | 2.19 | -1.44 | -0.33 | -2.77 | 1.12 |
Nucleus | 6.09 | 1.67 | 2.17 | -22.55 | 2.13 | 2.62 | -13.71 | -10.14 | -10.38 | 5.25 | 5.65 | -21.76 | -18.85 | -27.00 | 4.00 |
Peroxisomes | -5.96 | -4.20 | -1.37 | 5.78 | 4.48 | 5.89 | -1.69 | -3.91 | -5.74 | -2.58 | 3.79 | -0.56 | 0.70 | -2.88 | 1.33 |
Vacuole | 0.09 | -10.10 | -20.09 | -25.16 | -14.84 | -4.05 | -14.25 | -13.48 | -12.60 | -3.20 | -3.67 | -19.78 | -29.36 | -27.98 | -14.40 |
Vacuole Periphery | 2.49 | 3.82 | 3.72 | 2.11 | -0.29 | -2.11 | 12.39 | 15.36 | 20.82 | 3.09 | -1.03 | 13.09 | 12.25 | 15.72 | 0.34 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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