Standard name
Human Ortholog
Description Subunit of ISW2/yCHRAC chromatin accessibility complex; ISW2/yCHRAC also includes Itc1p, Isw2p, and Dpb4p; involved in inheritance of telomeric silencing; DLS1 has a paralog, DPB3, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 – – 0 0 0 0 – 0 0 0 0 – 0 – 0 0 0 0 0 0 0 0 0
Bud 0 – – 0 0 0 0 – 0 0 0 0 – 0 – 0 0 0 0 0 0 0 0 0
Bud Neck 0 – – 0 0 0 0 – 0 0 0 0 – 0 – 0 0 0 0 0 0 0 0 0
Bud Site 0 – – 0 0 0 0 – 0 0 0 0 – 0 – 0 0 0 – – – – – –
Cell Periphery 0 – – 0 0 0 0 – 0 0 0 0 – 0 – 0 0 0 0 0 0 0 0 0
Cytoplasm 0 – – 0.06 0.06 0 0 – 0 0 0 0 – 0.05 – 0 0 0 0 0 0 0 0 0
Endoplasmic Reticulum 0 – – 0 0 0 0 – 0 0 0 0 – 0 – 0 0 0 0 0 0 0 0 0
Endosome 0 – – 0 0 0 0 – 0 0 0 0 – 0 – 0 0 0 0 0 0 0 0 0
Golgi 0 – – 0 0 0 0 – 0 0 0 0 – 0 – 0 0 0 0 0 0 0 0 0
Mitochondria 0 – – 0 0 0.21 0 – 0.2 0.1 0.18 0.18 – 0.05 – 0 0 0 0 0 0 0 0 0
Nucleus 0.91 – – 0.94 0.92 0.91 0.95 – 0.88 0.89 0.88 0.84 – 0.95 – 0.91 0.92 0.89 0.87 0.85 0.88 0.9 0.87 0.78
Nuclear Periphery 0 – – 0 0 0 0 – 0 0 0 0 – 0 – 0 0 0 0 0 0 0 0 0
Nucleolus 0.12 – – 0 0 0 0.05 – 0.12 0.1 0.15 0.13 – 0.25 – 0.07 0.06 0.08 0.06 0 0.09 0.08 0.09 0.11
Peroxisomes 0 – – 0 0 0 0 – 0 0 0 0 – 0 – 0 0 0 0 0 0 0 0 0
SpindlePole 0 – – 0 0 0 0 – 0 0 0 0 – 0 – 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 – – 0 0 0 0 – 0 0 0 0 – 0 – 0 0 0 0 0 0 0 0 0.07
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 – – 0 0 0 0 – 0 0 0 0 – 0 – 0 0 0 0 0 0 0 0 0
Bud 0 – – 0 0 1 1 – 1 0 1 2 – 0 – 1 0 0 3 0 0 2 0 6
Bud Neck 0 – – 0 0 0 1 – 0 0 0 2 – 0 – 0 0 0 0 0 0 0 1 0
Bud Site 1 – – 0 0 4 2 – 6 2 2 5 – 0 – 0 0 0 – – – – – –
Cell Periphery 0 – – 0 0 0 0 – 0 0 0 0 – 0 – 0 0 0 0 0 0 0 0 0
Cytoplasm 5 – – 15 20 9 6 – 5 6 4 4 – 1 – 6 6 6 0 0 0 0 0 0
Endoplasmic Reticulum 0 – – 0 0 0 0 – 0 0 0 0 – 0 – 1 1 2 0 0 0 0 0 0
Endosome 0 – – 1 1 0 3 – 0 0 0 0 – 0 – 2 3 1 1 0 0 0 0 2
Golgi 0 – – 0 0 0 0 – 0 0 0 0 – 0 – 1 2 0 0 0 0 0 0 2
Mitochondria 2 – – 2 1 66 13 – 61 34 43 57 – 1 – 3 3 7 0 0 0 0 0 1
Nucleus 181 – – 238 319 291 505 – 264 294 206 274 – 19 – 249 255 177 177 22 40 272 400 423
Nuclear Periphery 0 – – 0 2 2 9 – 2 1 1 6 – 0 – 1 1 0 0 0 0 0 0 0
Nucleolus 23 – – 3 7 13 24 – 36 34 35 42 – 5 – 20 18 16 11 0 4 22 42 59
Peroxisomes 1 – – 0 0 0 0 – 0 0 0 0 – 0 – 0 0 1 0 0 0 0 0 0
SpindlePole 1 – – 4 2 1 10 – 2 1 0 3 – 0 – 1 2 1 0 0 0 0 2 5
Vac/Vac Membrane 3 – – 2 4 0 4 – 0 2 1 3 – 0 – 6 6 3 5 0 0 3 13 39
Unique Cell Count 198 254 348 321 532 300 331 235 325 20 273 277 200 205 27 46 301 463 542
Labelled Cell Count 217 265 356 387 578 377 374 293 398 26 291 297 214 205 27 46 301 463 542


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 8.6 – – 7.7 8.4 5.1 6.3 – 5.5 5.9 5.4 5.7 – 7.5 – 10.7 11.2 10.9 7.8 8.1 8.1
Std Deviation (1e-4) 1.8 – – 1.6 2.1 1.3 1.2 – 1.2 1.3 1.1 1.3 – 1.7 – 2.7 2.5 2.5 1.7 1.6 1.7
Intensity Change (Log2) – – – – – – – – – – – – – – – – – – – – –


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 2.1804 1.8878 1.6007 1.5297 1.3371 1.4495 3.0565 2.8024 2.8653 2.7329 2.1871 2.8171 4.4517 4.4413 3.7944 4.1694 4.4331 4.3302
Actin 0.0263 0 0.0109 0.013 0.0136 0.0008 0.0057 0 0 0 0 0 0.0367 0 0.0135 0.0002 0.0042 0
Bud 0.0003 0 0.0003 0.0004 0.0004 0.0001 0.0001 0 0 0 0 0 0.0007 0 0.0003 0.0002 0.0008 0
Bud Neck 0.0015 0.0001 0.0016 0.0167 0.0006 0.0011 0.0003 0 0 0 0.0002 0.0006 0.0034 0 0.0006 0.0002 0.0021 0.0006
Bud Periphery 0.0005 0 0.0004 0.0008 0.0009 0.0004 0.0001 0 0 0 0 0 0.0017 0 0.0008 0.0002 0.0029 0
Bud Site 0.0017 0 0.0037 0.0011 0.0009 0.0004 0.0003 0 0 0 0 0 0.0058 0.0001 0.0011 0.0016 0.0022 0
Cell Periphery 0.0001 0 0.0002 0.0002 0.0001 0.0001 0 0 0 0 0 0 0.0004 0 0.0002 0.0002 0.0006 0
Cytoplasm 0.0012 0 0.002 0.0008 0.0004 0.0006 0.0026 0.0006 0.0001 0 0 0 0.0009 0 0.0032 0.0023 0.0205 0
Cytoplasmic Foci 0.0035 0 0.0026 0.0013 0.0026 0.0007 0.0059 0 0 0 0 0 0.0155 0 0.0032 0.0035 0.0138 0
Eisosomes 0.0002 0 0.0003 0.0001 0.0002 0 0.0005 0 0 0 0 0 0.0009 0 0.0001 0 0.0001 0
Endoplasmic Reticulum 0.0011 0 0.0021 0.0004 0.0012 0.0006 0.0013 0.0001 0 0 0 0 0.0007 0 0.0016 0.0029 0.003 0
Endosome 0.007 0 0.0055 0.0057 0.0116 0.004 0.0065 0.0003 0 0 0 0 0.0101 0 0.0062 0.0492 0.023 0
Golgi 0.0027 0 0.0015 0.0062 0.0052 0.0005 0.0013 0 0 0 0 0 0.0084 0 0.0017 0.0021 0.0018 0
Lipid Particles 0.0049 0 0.0022 0.0007 0.0113 0.0019 0.0044 0 0 0 0 0 0.011 0 0.0034 0.008 0.005 0
Mitochondria 0.0027 0.0001 0.0074 0.0039 0.0048 0.0059 0.0012 0.0001 0.0001 0 0.0002 0 0.0036 0.0001 0.0057 0.0016 0.0019 0.0001
None 0.0014 0 0.0037 0.0001 0.0012 0.0003 0.0078 0.0001 0 0 0 0 0.0012 0 0.0006 0.0009 0.0081 0
Nuclear Periphery 0.0076 0.001 0.0091 0.004 0.0183 0.0062 0.0071 0.0045 0.0027 0.0001 0.0004 0.0001 0.0023 0.0002 0.0084 0.0102 0.0042 0.0001
Nucleolus 0.0175 0.0134 0.008 0.013 0.0714 0.0341 0.0119 0.0123 0.0071 0.0055 0.0434 0.0407 0.0469 0.0156 0.0135 0.0109 0.0786 0.0267
Nucleus 0.9045 0.9849 0.9256 0.9275 0.8441 0.936 0.9247 0.973 0.9896 0.9942 0.9553 0.9575 0.8192 0.9834 0.9184 0.885 0.8094 0.9718
Peroxisomes 0.003 0 0.0008 0.0009 0.0025 0.0004 0.0032 0 0 0 0 0 0.0134 0 0.0076 0.0003 0.0017 0
Punctate Nuclear 0.0108 0.0006 0.0103 0.0012 0.0064 0.0044 0.0141 0.0081 0.0002 0.0001 0.0003 0.0009 0.0158 0.0004 0.0065 0.0017 0.0134 0.0006
Vacuole 0.0008 0 0.0009 0.0012 0.0009 0.0009 0.0005 0.0006 0.0001 0 0.0001 0 0.001 0 0.0023 0.0146 0.0023 0
Vacuole Periphery 0.0007 0 0.0008 0.0005 0.0014 0.0007 0.0004 0.0003 0.0001 0 0 0 0.0005 0 0.001 0.0044 0.0005 0

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 33.9341 19.571 18.8792 26.4546 26.9659 30.6332 33.6399 34.7248 30.3263 25.0247
Translational Efficiency 0.4749 1.0028 0.9283 0.7354 0.6803 0.6607 0.6315 0.5976 0.7196 0.6596

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Subunit of ISW2/yCHRAC chromatin accessibility complex; ISW2/yCHRAC also includes Itc1p, Isw2p, and Dpb4p; involved in inheritance of telomeric silencing; DLS1 has a paralog, DPB3, that arose from the whole genome duplication
Localization
Cell Percentages nucleus (99%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-4

Dls1

Dls1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Dls1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available