Standard name | |
---|---|
Human Ortholog | |
Description | Cell wall-associated protein involved in export of acetylated sterols; member of the CAP protein superfamily (cysteine-rich secretory proteins (CRISP), antigen 5, and pathogenesis related 1 proteins); role in mating efficiency; expression of full-length transcript is daughter cell-specific; in response to alpha factor, a short transcript starting at +452 is expressed and the long form is repressed by Ste12p |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 26 | 29 | 33 | 34 | 28 | 28 | 37 | 38 | 10 | – | 15 | 17 | 54 | 10 | 20 | 7 | 12 | 3 | 10 | 15 | 15 | 24 | 57 | 25 |
Bud | 12 | 11 | 10 | 12 | 5 | 9 | 12 | 13 | 4 | – | 8 | 12 | 13 | 4 | 5 | 1 | 3 | 6 | 34 | 46 | 45 | 38 | 46 | 49 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 5 | 7 | 10 | 18 | 9 |
Bud Site | 0 | 0 | 7 | 1 | 0 | 1 | 0 | 0 | 1 | – | 1 | 0 | 4 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 8 | 25 | 17 | 15 | 27 | 19 | 30 | 23 | 13 | – | 9 | 16 | 46 | 42 | 44 | 15 | 45 | 35 | 2 | 4 | 2 | 9 | 25 | 24 |
Cytoplasm | 67 | 45 | 38 | 49 | 51 | 58 | 84 | 81 | 63 | – | 74 | 55 | 41 | 64 | 84 | 12 | 46 | 33 | 6 | 6 | 6 | 3 | 4 | 6 |
Endoplasmic Reticulum | 123 | 125 | 118 | 101 | 132 | 151 | 175 | 180 | 86 | – | 63 | 64 | 152 | 121 | 129 | 2 | 6 | 4 | 150 | 134 | 148 | 115 | 157 | 148 |
Endosome | 3 | 0 | 0 | 3 | 1 | 1 | 3 | 0 | 0 | – | 0 | 3 | 1 | 5 | 2 | 0 | 0 | 0 | 1 | 1 | 0 | 1 | 0 | 1 |
Golgi | 1 | 2 | 0 | 2 | 2 | 0 | 0 | 2 | 1 | – | 0 | 1 | 0 | 2 | 0 | 2 | 13 | 18 | 3 | 1 | 0 | 1 | 3 | 0 |
Mitochondria | 5 | 5 | 0 | 1 | 3 | 12 | 15 | 10 | 30 | – | 22 | 23 | 3 | 2 | 0 | 4 | 2 | 0 | 7 | 5 | 8 | 7 | 9 | 9 |
Nucleus | 0 | 0 | 2 | 1 | 0 | 2 | 1 | 2 | 0 | – | 0 | 2 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 |
Nuclear Periphery | 0 | 1 | 0 | 2 | 1 | 0 | 6 | 1 | 5 | – | 6 | 9 | 1 | 0 | 0 | 0 | 0 | 0 | 5 | 3 | 6 | 1 | 0 | 4 |
Nucleolus | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 1 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
Peroxisomes | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 1 | 1 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
SpindlePole | 1 | 4 | 8 | 3 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 2 | 4 | 4 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 |
Vac/Vac Membrane | 6 | 15 | 25 | 19 | 14 | 39 | 36 | 85 | 45 | – | 28 | 41 | 0 | 6 | 8 | 5 | 7 | 8 | 7 | 13 | 10 | 5 | 6 | 10 |
Unique Cell Count | 204 | 207 | 204 | 190 | 219 | 258 | 310 | 348 | 217 | 194 | 196 | 232 | 206 | 250 | 40 | 114 | 97 | 236 | 240 | 256 | 221 | 335 | 300 | |
Labelled Cell Count | 252 | 265 | 258 | 243 | 264 | 320 | 399 | 435 | 258 | 227 | 243 | 319 | 261 | 298 | 49 | 136 | 107 | 236 | 240 | 256 | 221 | 335 | 300 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 14.8 | 14.8 | 13.7 | 13.1 | 13.1 | 11.6 | 11.7 | 12.1 | 10.5 | – | 9.8 | 9.9 | 14.7 | 13.8 | 12.8 | 31.7 | 32.2 | 32.5 | 11.9 | 11.4 | 11.1 |
Std Deviation (1e-4) | 7.2 | 7.1 | 5.9 | 5.7 | 4.8 | 3.8 | 3.9 | 3.7 | 3.9 | – | 3.4 | 3.5 | 7.5 | 6.6 | 6.1 | 18.9 | 18.2 | 18.1 | 6.2 | 6.0 | 5.2 |
Intensity Change (Log2) | – | – | – | -0.07 | -0.07 | -0.24 | -0.23 | -0.18 | -0.38 | – | -0.48 | -0.46 | 0.11 | 0.02 | -0.1 | 1.21 | 1.24 | 1.25 | -0.2 | -0.26 | -0.3 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0.5 | -1.0 | -1.7 | -1.4 | -1.8 | -3.9 | – | -2.6 | -2.3 | 1.9 | -3.7 | -2.7 | 0.2 | -1.4 | -3.3 |
Bud | 0.6 | -1.5 | -0.8 | -0.6 | -0.7 | -1.7 | – | -0.4 | 0.5 | 0.3 | -1.7 | -1.7 | 0 | 0 | 0.5 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | -1.1 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | -0.2 | 1.3 | -0.4 | 0.5 | -0.8 | -0.9 | – | -1.5 | -0.1 | 3.4 | 3.5 | 2.9 | 5.0 | 6.7 | 6.0 |
Cytoplasm | 1.7 | 1.2 | 1.0 | 2.2 | 1.3 | 2.5 | – | 4.3 | 2.2 | -0.3 | 2.9 | 3.6 | 1.6 | 4.2 | 2.9 |
Endoplasmic Reticulum | -0.9 | 0.5 | 0.1 | -0.3 | -1.4 | -3.7 | – | -5.1 | -5.1 | 1.6 | 0.2 | -1.3 | -6.1 | -9.2 | -8.9 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 6.3 |
Mitochondria | 0 | 0 | 3.1 | 3.2 | 0 | 5.5 | – | 4.9 | 5.0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | -1.4 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | -1.2 | -1.5 | 0 | 0 | 0 |
Vacuole | -0.7 | -2.1 | 0.9 | -0.2 | 3.5 | 2.3 | – | 0.6 | 2.3 | -5.5 | -3.6 | -3.7 | 0 | -1.7 | -1.0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 23.3024 | 12.4913 | 12.9235 | 10.1121 | 11.2532 | 13.4323 | 22.4672 | 11.3045 | 12.2494 | 14.8218 | 10.54 | 13.2942 | 27.2562 | 11.8014 | 12.5058 | 13.3509 | 12.7587 | 14.0601 |
Actin | 0.0331 | 0.0239 | 0.0833 | 0.0415 | 0.0169 | 0.0458 | 0.0453 | 0.0298 | 0.0621 | 0.0148 | 0.0266 | 0.0458 | 0.0315 | 0.0257 | 0.0314 | 0.0124 | 0.0295 | 0.0431 |
Bud | 0.0012 | 0.008 | 0.0079 | 0.0031 | 0.0017 | 0.0029 | 0.001 | 0.0097 | 0.0081 | 0.0023 | 0.0071 | 0.0034 | 0.0019 | 0.0186 | 0.0119 | 0.0076 | 0.0236 | 0.0042 |
Bud Neck | 0.004 | 0.0037 | 0.0083 | 0.0301 | 0.0278 | 0.0267 | 0.0028 | 0.0056 | 0.0096 | 0.0029 | 0.0076 | 0.0203 | 0.0064 | 0.0026 | 0.0059 | 0.002 | 0.009 | 0.0358 |
Bud Periphery | 0.0062 | 0.0179 | 0.0439 | 0.0274 | 0.0139 | 0.0356 | 0.0063 | 0.0446 | 0.035 | 0.0305 | 0.0451 | 0.0274 | 0.0184 | 0.063 | 0.055 | 0.0536 | 0.0857 | 0.0439 |
Bud Site | 0.0046 | 0.1366 | 0.0436 | 0.0033 | 0.0033 | 0.002 | 0.0064 | 0.0656 | 0.0209 | 0.0006 | 0.0102 | 0.0013 | 0.0047 | 0.0665 | 0.0245 | 0.0015 | 0.0048 | 0.0016 |
Cell Periphery | 0.0385 | 0.0621 | 0.0414 | 0.0216 | 0.0415 | 0.0288 | 0.0387 | 0.1002 | 0.0534 | 0.0396 | 0.0663 | 0.0325 | 0.0416 | 0.0931 | 0.0714 | 0.0449 | 0.0286 | 0.0505 |
Cytoplasm | 0.0267 | 0.0768 | 0.018 | 0.0379 | 0.0497 | 0.0459 | 0.019 | 0.068 | 0.0379 | 0.0209 | 0.027 | 0.0462 | 0.0242 | 0.1008 | 0.0424 | 0.0228 | 0.038 | 0.0459 |
Cytoplasmic Foci | 0.0117 | 0.0058 | 0.0026 | 0.004 | 0.0151 | 0.0038 | 0.0214 | 0.0041 | 0.0059 | 0.0006 | 0.0049 | 0.0015 | 0.0063 | 0.0022 | 0.002 | 0.0019 | 0.0024 | 0.0032 |
Eisosomes | 0.009 | 0.001 | 0.0024 | 0.0008 | 0.0008 | 0.0012 | 0.0063 | 0.0051 | 0.0022 | 0.0005 | 0.0005 | 0.0014 | 0.0015 | 0.0043 | 0.0027 | 0.0074 | 0.0017 | 0.0024 |
Endoplasmic Reticulum | 0.6927 | 0.5712 | 0.6415 | 0.6702 | 0.6555 | 0.7288 | 0.7043 | 0.5966 | 0.6555 | 0.8479 | 0.6351 | 0.7571 | 0.7233 | 0.5062 | 0.6604 | 0.7408 | 0.7019 | 0.6921 |
Endosome | 0.0248 | 0.0207 | 0.0145 | 0.0264 | 0.0451 | 0.0127 | 0.0219 | 0.0073 | 0.0141 | 0.0037 | 0.0644 | 0.0123 | 0.0174 | 0.0057 | 0.0042 | 0.0038 | 0.0055 | 0.0078 |
Golgi | 0.024 | 0.006 | 0.0223 | 0.0096 | 0.0187 | 0.0134 | 0.0315 | 0.0064 | 0.0153 | 0.0066 | 0.0149 | 0.0093 | 0.0136 | 0.0034 | 0.0085 | 0.0264 | 0.0054 | 0.0118 |
Lipid Particles | 0.0194 | 0.0105 | 0.0117 | 0.0353 | 0.0074 | 0.0176 | 0.0285 | 0.0154 | 0.0221 | 0.0036 | 0.0102 | 0.009 | 0.0117 | 0.0168 | 0.0181 | 0.0264 | 0.0091 | 0.0065 |
Mitochondria | 0.009 | 0.0021 | 0.0019 | 0.0021 | 0.0029 | 0.0032 | 0.0045 | 0.0007 | 0.0086 | 0.0006 | 0.0015 | 0.0042 | 0.0068 | 0.0012 | 0.0028 | 0.0005 | 0.0151 | 0.0063 |
None | 0.0061 | 0.0024 | 0.0017 | 0.0011 | 0.0587 | 0.0016 | 0.0018 | 0.0032 | 0.0012 | 0.0003 | 0.001 | 0.0014 | 0.0097 | 0.0058 | 0.0057 | 0.0015 | 0.012 | 0.0054 |
Nuclear Periphery | 0.0433 | 0.0229 | 0.0357 | 0.0493 | 0.0154 | 0.0153 | 0.0358 | 0.0162 | 0.0203 | 0.0134 | 0.0317 | 0.0127 | 0.0495 | 0.0374 | 0.025 | 0.0291 | 0.0148 | 0.0184 |
Nucleolus | 0.0007 | 0.0005 | 0.0004 | 0.0002 | 0.0004 | 0.0002 | 0.0002 | 0.0005 | 0.0004 | 0.0001 | 0.0008 | 0.0001 | 0.0004 | 0.0009 | 0.0023 | 0.0002 | 0.001 | 0.0003 |
Nucleus | 0.0035 | 0.0051 | 0.003 | 0.0032 | 0.0032 | 0.0021 | 0.0032 | 0.0023 | 0.0028 | 0.0011 | 0.008 | 0.0021 | 0.0047 | 0.0096 | 0.0082 | 0.0022 | 0.0029 | 0.0037 |
Peroxisomes | 0.003 | 0.0005 | 0.0002 | 0.0002 | 0.0005 | 0.0011 | 0.0058 | 0.0001 | 0.0009 | 0 | 0.0003 | 0.0001 | 0.0011 | 0.0003 | 0.0002 | 0 | 0.0005 | 0.0002 |
Punctate Nuclear | 0.0088 | 0.0009 | 0.0008 | 0.0006 | 0.0021 | 0.0008 | 0.0013 | 0.0006 | 0.0042 | 0.0001 | 0.0023 | 0.0003 | 0.0029 | 0.0018 | 0.0015 | 0.0006 | 0.0011 | 0.0005 |
Vacuole | 0.0218 | 0.0181 | 0.0125 | 0.0228 | 0.0164 | 0.0089 | 0.0112 | 0.0167 | 0.0162 | 0.0084 | 0.0296 | 0.0097 | 0.0179 | 0.0305 | 0.0143 | 0.0128 | 0.0055 | 0.0146 |
Vacuole Periphery | 0.0078 | 0.0034 | 0.0026 | 0.0092 | 0.0029 | 0.0017 | 0.0029 | 0.001 | 0.0033 | 0.0014 | 0.0048 | 0.0018 | 0.0047 | 0.0035 | 0.0016 | 0.0017 | 0.002 | 0.002 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 3.3361 | 4.1006 | 5.809 | 23.9773 | 38.3876 | 7.9188 | 4.7115 | 4.7063 | 14.8063 | 68.6169 |
Translational Efficiency | – | – | – | – | – | – | – | – | – | – |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✔ |
Translational Efficiency | – |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
2348 | 1223 | 1376 | 1956 | 1715 | 1402 | 1500 | 175 | 4063 | 2625 | 2876 | 2131 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 1264.34 | 1477.49 | 2431.35 | 1683.61 | 1361.63 | 1406.85 | 1906.01 | 1459.79 | 1305.41 | 1439.76 | 2157.35 | 1665.23 |
Standard Deviation | 610.75 | 601.92 | 1104.28 | 659.24 | 524.03 | 544.18 | 782.03 | 539.73 | 577.74 | 572.89 | 985.53 | 653.15 |
Intensity Change Log 2 | — | 0.224764 | 0.943373 | 0.413174 | — | 0.047134 | 0.485221 | 0.100426 | — | 0.135391 | 0.723967 | 0.259475 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.009547 | 0.008665 | 0.004068 | 0.007589 | 0.009408 | 0.014223 | 0.003807 | 0.010094 | 0.009488 | 0.011633 | 0.003932 | 0.007795 |
Bud Neck | 0.016764 | 0.009716 | 0.023249 | 0.011827 | 0.009744 | 0.010720 | 0.012219 | 0.010712 | 0.013801 | 0.010252 | 0.017496 | 0.011735 |
Bud Site | 0.197312* | 0.108716* | 0.062040 | 0.095926 | 0.105137* | 0.141858* | 0.056437 | 0.066064 | 0.158405* | 0.126417* | 0.059118 | 0.093474 |
Cell Periphery | 0.108981* | 0.071368 | 0.164175* | 0.059138 | 0.070299 | 0.037800 | 0.025886 | 0.017339 | 0.092654 | 0.053439 | 0.092049 | 0.055705 |
Cytoplasm | 0.057064 | 0.031336 | 0.016481 | 0.072196 | 0.033262 | 0.061529 | 0.032313 | 0.074104 | 0.047017 | 0.047462 | 0.024738 | 0.072352 |
Cytoplasmic Foci | 0.008897 | 0.008806 | 0.002612 | 0.006886 | 0.005683 | 0.019297 | 0.004610 | 0.018698 | 0.007540 | 0.014409 | 0.003654 | 0.007856 |
Eisosomes | 0.001064 | 0.001030 | 0.001065 | 0.000425 | 0.001054 | 0.000562 | 0.000210 | 0.000309 | 0.001060 | 0.000780 | 0.000619 | 0.000415 |
Endoplasmic Reticulum | 0.494367* | 0.680780* | 0.508875* | 0.613972* | 0.700192* | 0.605939* | 0.750495* | 0.607324* | 0.581246* | 0.640808* | 0.634894* | 0.613426* |
Endosome | 0.016451 | 0.017175 | 0.016299 | 0.012202 | 0.014173 | 0.025881 | 0.017478 | 0.022320 | 0.015490 | 0.021825 | 0.016914 | 0.013033 |
Golgi | 0.002646 | 0.005106 | 0.005914 | 0.005929 | 0.003856 | 0.008790 | 0.001693 | 0.004237 | 0.003157 | 0.007073 | 0.003712 | 0.005790 |
Lipid Particles | 0.006572 | 0.012172 | 0.040227 | 0.026127 | 0.009460 | 0.012949 | 0.011144 | 0.051631 | 0.007791 | 0.012587 | 0.025059 | 0.028221 |
Mitochondria | 0.012987 | 0.004491 | 0.003993 | 0.003919 | 0.004538 | 0.008781 | 0.002927 | 0.000750 | 0.009421 | 0.006782 | 0.003437 | 0.003659 |
Mitotic Spindle | 0.000565 | 0.001505 | 0.000797 | 0.003959 | 0.000387 | 0.001809 | 0.001488 | 0.003637 | 0.000490 | 0.001667 | 0.001157 | 0.003933 |
None | 0.008767 | 0.002418 | 0.002521 | 0.001855 | 0.002669 | 0.005526 | 0.002606 | 0.000466 | 0.006193 | 0.004078 | 0.002565 | 0.001741 |
Nuclear Periphery | 0.000902 | 0.000652 | 0.008415 | 0.001786 | 0.000512 | 0.000709 | 0.006177 | 0.002013 | 0.000737 | 0.000682 | 0.007248 | 0.001805 |
Nuclear Periphery Foci | 0.003756 | 0.005031 | 0.015735 | 0.009289 | 0.002525 | 0.006720 | 0.021666 | 0.023691 | 0.003237 | 0.005933 | 0.018829 | 0.010471 |
Nucleolus | 0.000276 | 0.000148 | 0.000147 | 0.000107 | 0.000084 | 0.000146 | 0.000106 | 0.000048 | 0.000195 | 0.000147 | 0.000126 | 0.000102 |
Nucleus | 0.006720 | 0.002345 | 0.005771 | 0.010967 | 0.002281 | 0.004018 | 0.005967 | 0.008120 | 0.004846 | 0.003239 | 0.005873 | 0.010734 |
Peroxisomes | 0.001686 | 0.001310 | 0.000138 | 0.000520 | 0.001666 | 0.002096 | 0.000137 | 0.000840 | 0.001677 | 0.001730 | 0.000138 | 0.000546 |
Vacuole | 0.037308 | 0.022446 | 0.110148 | 0.051495 | 0.019716 | 0.025949 | 0.040706 | 0.075843 | 0.029883 | 0.024317 | 0.073930 | 0.053495 |
Vacuole Periphery | 0.007367 | 0.004784 | 0.007331 | 0.003886 | 0.003352 | 0.004700 | 0.001928 | 0.001762 | 0.005672 | 0.004739 | 0.004513 | 0.003711 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | 0.72 | 7.29 | 0.59 | -0.13 | -4.40 | -2.77 | 4.36 | -0.21 | 1.00 | -1.48 | -2.13 | 7.96 | 0.44 | 2.12 | -4.74 |
Bud Neck | 5.03 | -5.30 | 3.15 | -2.39 | 7.62 | -0.84 | -2.91 | -0.86 | -0.44 | 0.67 | 3.77 | -5.21 | 0.94 | -2.48 | 5.58 |
Bud Site | 10.12 | 18.04* | 12.51 | 0.54 | -5.88 | -4.41 | 6.42 | 2.57 | 5.08 | -0.65 | 5.16 | 18.45 | 9.52 | 3.91 | -7.00 |
Cell Periphery | 7.61 | -8.84 | 11.94 | 3.15 | 18.44 | 8.77 | 12.26 | 13.59 | 6.45 | 3.24 | 12.58 | 0.35 | 11.19 | -0.08 | 9.81 |
Cytoplasm | 6.42 | 11.17 | -5.52 | -11.25 | -15.63 | -6.32 | -0.81 | -4.27 | -1.95 | -4.01 | -0.23 | 7.18 | -8.91 | -8.32 | -14.01 |
Cytoplasmic Foci | 0.04 | 6.49 | 1.28 | 0.89 | -5.18 | -7.41 | 0.81 | -3.49 | -0.08 | -3.66 | -5.75 | 4.92 | -1.16 | 4.48 | -5.10 |
Eisosomes | 0.20 | 0.03 | 5.94 | 4.78 | 6.60 | 4.06 | 7.60 | 6.17 | 3.11 | -1.71 | 2.88 | 5.16 | 7.84 | 4.90 | 3.46 |
Endoplasmic Reticulum | -14.41 | 0.63 | -7.11 | 7.46 | -7.06 | 7.24 | -2.13 | 4.57 | 1.46 | 5.37 | -6.19 | -3.33 | 0.40 | 5.56 | 3.14 |
Endosome | -0.61 | -0.72 | 4.71 | 3.97 | 5.51 | -8.02 | -3.86 | -2.77 | 1.38 | -1.17 | -6.52 | -3.23 | 3.15 | 8.44 | 5.86 |
Golgi | -3.52 | -7.11 | -3.44 | -0.47 | 0.23 | -4.54 | 4.01 | -0.05 | 3.76 | -2.46 | -5.99 | -1.61 | -2.90 | 1.61 | -2.23 |
Lipid Particles | -3.86 | -12.02 | -10.66 | -6.32 | 4.58 | -2.44 | -1.72 | -4.51 | -4.10 | -4.18 | -4.76 | -11.28 | -11.11 | -7.82 | -0.86 |
Mitochondria | 7.92 | 8.78 | 9.30 | 0.73 | 1.00 | -3.32 | 2.29 | 8.42 | 6.57 | 7.32 | 2.98 | 9.18 | 9.07 | 4.02 | 0.44 |
Mitotic Spindle | -1.64 | -1.98 | -4.43 | -2.77 | -3.90 | -4.31 | -3.24 | -1.95 | -1.23 | -1.19 | -3.67 | -3.67 | -4.81 | -3.12 | -3.50 |
None | 6.28 | 6.69 | 7.79 | 0.87 | 1.39 | -2.56 | 0.01 | 2.95 | 4.95 | 3.14 | 2.67 | 5.50 | 7.19 | 3.66 | 1.90 |
Nuclear Periphery | 2.86 | -16.41 | -6.53 | -9.20 | 14.23 | -2.00 | -9.25 | -4.66 | -3.93 | 5.92 | 0.74 | -16.79 | -9.14 | -9.26 | 13.61 |
Nuclear Periphery Foci | -1.48 | -10.08 | -7.41 | -3.95 | 4.93 | -5.07 | -11.67 | -5.64 | -4.50 | -0.61 | -4.35 | -15.16 | -9.92 | -5.19 | 6.73 |
Nucleolus | 3.25 | 4.64 | 6.76 | 1.04 | 3.24 | -2.86 | -0.99 | 0.69 | 2.73 | 1.13 | 1.99 | 0.79 | 5.41 | 1.81 | 1.17 |
Nucleus | 9.15 | -1.09 | -7.25 | -11.77 | -5.90 | -3.91 | -8.02 | -3.87 | -3.15 | -1.97 | 3.98 | -5.18 | -9.74 | -11.16 | -6.60 |
Peroxisomes | 1.14 | 6.81 | 5.06 | 3.41 | -7.76 | -0.82 | 8.27 | 2.66 | 2.26 | -2.85 | -0.17 | 10.02 | 7.19 | 4.17 | -7.47 |
Vacuole | 6.10 | -19.88 | -7.86 | -12.30 | 12.52 | -2.86 | -9.74 | -6.24 | -5.72 | -4.06 | 3.24 | -20.09 | -12.25 | -13.90 | 4.74 |
Vacuole Periphery | 2.25 | -0.03 | 4.30 | 1.03 | 5.95 | -2.06 | 2.78 | 2.08 | 3.42 | 0.50 | 1.40 | 2.07 | 3.49 | 1.80 | 2.12 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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