Standard name
Human Ortholog
Description DNA replication initiation protein; loads DNA pol epsilon onto pre-replication complexes at origins; checkpoint sensor recruited to stalled replication forks by the checkpoint clamp complex where it activates Mec1p; along with Rfa1p, binds to ultrafine anaphase bridges in mitotic cells and prevents accumulation of chromatin bridges by stimulating the Mec1p kinase and suppressing homologous recombination; ortholog of human TopBP1; forms nuclear foci upon DNA replication stress
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0.05 0.05 0 0 0 0.05 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.33 0.4 0.53 0.4 0.51 0.54 0.5 0.47 0.36 0.3 0.25 0.25 0.31 0.38 0.43 0.63 0.61 0.66 0.24 0.2 0.25 0.24 0.35 0.44
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0.1 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.25 0.2 0.05 0.24 0.16 0.29 0.42 0.28 0.67 0.64 0.71 0.61 0.2 0.06 0.16 0 0 0 0.06 0 0.05 0.09 0.09 0.1
Nucleus 0.29 0.28 0.34 0.4 0.26 0.2 0.24 0.25 0.21 0.28 0.31 0.34 0.37 0.25 0.3 0.07 0.06 0.14 0.33 0.34 0.27 0.17 0.06 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0
Vac/Vac Membrane 0.25 0.26 0.06 0 0.09 0.06 0 0.06 0 0 0 0 0.24 0.29 0.24 0.41 0.41 0.28 0.27 0.29 0.32 0.38 0.38 0.27
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 3 3
Bud 2 1 0 1 1 1 3 0 9 3 6 6 1 0 0 0 0 0 1 3 2 6 9 6
Bud Neck 1 1 10 6 6 1 8 14 5 1 3 12 0 0 0 0 0 0 0 3 3 0 3 5
Bud Site 0 1 0 0 0 1 7 1 6 7 8 9 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 2 0 0 0 0 2 0 0 0 0 0 0 0 2 2 0 0 0 0 0 0 1
Cytoplasm 46 82 101 52 70 160 133 121 102 100 64 61 72 82 98 164 151 130 44 55 68 39 98 133
Endoplasmic Reticulum 2 0 0 0 0 0 0 0 0 0 1 0 1 0 3 12 8 4 1 0 1 0 3 5
Endosome 0 1 0 0 1 0 0 0 0 0 0 0 2 21 3 8 10 2 0 5 0 1 1 4
Golgi 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 2 0 0 1 0
Mitochondria 35 42 9 31 22 87 112 71 190 211 180 148 47 12 36 8 5 2 11 7 12 14 24 30
Nucleus 40 57 65 52 36 61 64 64 60 91 78 82 88 53 69 18 14 28 60 94 73 26 17 9
Nuclear Periphery 0 0 0 0 0 0 0 1 1 3 1 1 0 0 0 1 0 0 0 0 0 0 0 1
Nucleolus 0 0 0 0 0 0 0 0 1 0 0 0 2 4 0 0 1 1 0 0 0 0 1 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 4 0 2 1 5 5 4 2 2 2 3 1 0 0 0 0 0 7 14 5 2 5 9
Vac/Vac Membrane 35 53 11 4 13 17 8 16 6 11 8 4 56 63 55 108 101 54 50 80 84 61 106 80
Unique Cell Count 138 206 189 130 137 299 266 257 285 328 254 244 235 214 230 262 246 196 185 277 269 162 281 301
Labelled Cell Count 161 244 198 148 150 333 340 294 382 429 351 326 270 236 264 321 292 222 185 277 269 162 281 301


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.0 4.0 4.6 4.2 4.8 3.8 3.8 4.2 3.3 3.3 3.1 3.6 4.5 4.5 4.1 7.0 7.7 6.8 4.2 4.1 4.6
Std Deviation (1e-4) 0.7 0.8 1.0 1.2 1.4 1.3 1.2 1.4 0.8 1.1 0.9 0.9 1.5 1.3 1.3 1.5 2.0 1.7 1.8 1.6 1.9
Intensity Change (Log2) – – – -0.13 0.06 -0.26 -0.25 -0.14 -0.48 -0.48 -0.54 -0.36 -0.03 -0.02 -0.14 0.62 0.76 0.58 -0.11 -0.15 0.0

WT3RAP60RAP140RAP220RAP300RAP380RAP460RAP540RAP620RAP70002468WT3HU80HU120HU16002468WT3rpd3Δ_1rpd3Δ_2rpd3Δ_302468WT1AF100AF140AF18002468
rapamycinhydroxy-urearpd3 knockoutalpha-factorMultiple Condition Time-Course Intensity PlotsGFP Intensity (1e-4)GFP Intensity (1e-4)GFP Intensity (1e-4)GFP Intensity (1e-4)

Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – 0 0 0
Bud Neck – – – – – – – – – – – – 0 0 0
Bud Site – – – – – – – – – – – – 0 0 0
Cell Periphery – – – – – – – – – – – – 0 0 0
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – 0 0 0
Endosome – – – – – – – – – – – – 0 0 0
Golgi – – – – – – – – – – – – 0 0 0
Mitochondria – – – – – – – – – – – – 0 0 0
Nuclear Periphery – – – – – – – – – – – – 0 0 0
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – 0 0 0
Peroxisomes – – – – – – – – – – – – 0 0 0
SpindlePole – – – – – – – – – – – – 0 0 0
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – 0 0 0
Cytoplasm – – – – – – – – – – – – 0 0 0
Nucleus – – – – – – – – – – – – 0 0 0
Vacuole – – – – – – – – – – – – 8.4 8.3 5.7

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration -0.6069 -0.6193 -0.5326 -1.2208 -0.7732 -0.8288 0.0297 0.1845 0.2518 -0.3198 -0.2554 0.1699 -0.9014 -0.1766 -0.6635 -0.3951 -0.6146 -0.6176
Actin 0.0653 0.0008 0.0101 0.0006 0.0006 0.0123 0.0262 0.0004 0.0101 0.0377 0.0006 0.0171 0.0003 0.0043 0.001 0.0003 0.0004 0.0004
Bud 0.0012 0.0004 0.0009 0.0001 0 0.0014 0.001 0.0002 0.0003 0.0004 0.0006 0.0002 0.0001 0.0001 0.0005 0 0 0.0001
Bud Neck 0.0024 0.0008 0.005 0.0002 0.0006 0.0066 0.0033 0.0022 0.0015 0.0011 0.0032 0.005 0.0004 0.0003 0.0007 0.0005 0.0007 0.0033
Bud Periphery 0.0016 0.0005 0.0013 0.0001 0 0.0018 0.002 0.0002 0.0003 0.0004 0.0008 0.0003 0.0001 0.0001 0.0005 0 0 0.0001
Bud Site 0.0102 0.003 0.0041 0.0002 0.0027 0.0006 0.0056 0.0027 0.0088 0.0012 0.0052 0.0004 0.0003 0.0011 0.0028 0.0001 0.0001 0.0005
Cell Periphery 0.0007 0.0002 0.0002 0 0 0.0001 0.0006 0.0001 0.0002 0 0.0001 0.0001 0 0.0001 0 0 0 0
Cytoplasm 0.1494 0.255 0.1555 0.1482 0.1564 0.1498 0.1537 0.2616 0.1459 0.1317 0.1354 0.1284 0.1294 0.1169 0.0945 0.0724 0.0997 0.097
Cytoplasmic Foci 0.0336 0.0047 0.0258 0.0084 0.1006 0.0131 0.0277 0.0042 0.0109 0.0054 0.0294 0.0072 0.0069 0.0053 0.006 0.0015 0.0117 0.0036
Eisosomes 0.0007 0.0001 0.0002 0.0002 0.0001 0.0001 0.0005 0 0.0001 0.0001 0.0001 0.0001 0.0001 0.0001 0.0001 0.0001 0 0
Endoplasmic Reticulum 0.0071 0.0094 0.0069 0.0032 0.0453 0.0073 0.0082 0.0088 0.0041 0.0023 0.0019 0.002 0.0036 0.0028 0.0027 0.0009 0.0015 0.0014
Endosome 0.0482 0.0063 0.0326 0.0055 0.0042 0.0182 0.0404 0.0042 0.0121 0.0034 0.0622 0.0087 0.0076 0.0041 0.0067 0.0011 0.0037 0.0021
Golgi 0.0299 0.0003 0.0137 0.0007 0.0011 0.001 0.0149 0.0001 0.0092 0.0036 0.0319 0.0091 0.0002 0.0009 0.0002 0 0.0001 0.0001
Lipid Particles 0.0299 0.001 0.0069 0.0093 0.0102 0.0064 0.0188 0.0006 0.0081 0.0017 0.0291 0.0071 0.0015 0.001 0.0011 0.0002 0.0006 0.0003
Mitochondria 0.027 0.0056 0.0098 0.1042 0.0003 0.0048 0.0073 0.0006 0.0037 0.0015 0.0054 0.011 0.0008 0.0008 0.0008 0.0007 0.0006 0.0004
None 0.1662 0.2309 0.2673 0.4324 0.3836 0.0868 0.1579 0.1438 0.1593 0.1614 0.1636 0.0903 0.2099 0.1924 0.1845 0.2804 0.2354 0.137
Nuclear Periphery 0.0386 0.0361 0.0249 0.022 0.0382 0.0384 0.0477 0.03 0.0313 0.0188 0.016 0.0157 0.0296 0.016 0.0273 0.011 0.0112 0.0122
Nucleolus 0.0039 0.0059 0.0075 0.0057 0.0011 0.0061 0.0059 0.0038 0.0054 0.0052 0.0117 0.0064 0.0048 0.0039 0.0064 0.0077 0.0055 0.0093
Nucleus 0.3017 0.3943 0.3463 0.1765 0.2157 0.5912 0.4215 0.4957 0.5509 0.5737 0.4575 0.6295 0.558 0.6176 0.6159 0.5645 0.5374 0.6693
Peroxisomes 0.0076 0.0003 0.0191 0.0199 0.0049 0.0005 0.0186 0.0001 0.0076 0.0035 0.0101 0.004 0.0003 0.0039 0.0002 0.0001 0.0003 0.0002
Punctate Nuclear 0.0554 0.0389 0.0537 0.0607 0.0321 0.046 0.0289 0.0364 0.027 0.0453 0.0282 0.0549 0.0417 0.0252 0.0462 0.0577 0.0901 0.0615
Vacuole 0.0121 0.0049 0.007 0.0013 0.0017 0.0055 0.0068 0.0035 0.0024 0.0012 0.006 0.0022 0.0033 0.0029 0.0015 0.0005 0.0007 0.0008
Vacuole Periphery 0.0074 0.0008 0.0013 0.0007 0.0006 0.002 0.0025 0.0006 0.0009 0.0003 0.0011 0.0005 0.0007 0.0003 0.0004 0.0002 0.0002 0.0002

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 9.1349 6.0171 4.465 9.33 6.2336 2.8831 6.0839 9.5323 6.4302 10.6078
Translational Efficiency – – – – – – – – – –

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency –

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
2435 1563 163 1620 1861 1904 1867 1476 4296 3467 2030 3096

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 645.67 748.47 947.68 894.66 658.69 740.49 862.10 868.53 651.31 744.09 868.97 882.20
Standard Deviation 65.82 97.36 99.84 107.92 77.64 88.72 101.22 117.24 71.47 92.80 103.75 113.21
Intensity Change Log 2 — 0.213147 0.553603 0.470543 — 0.168881 0.388256 0.398976 — 0.190963 0.472472 0.434846

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000020 0.000322 0.000989 0.000884 0.000046 0.000372 0.000208 0.000434 0.000031 0.000349 0.000271 0.000669
Bud Neck 0.006187 0.007968 0.003321 0.017040 0.007006 0.011680 0.006059 0.008891 0.006542 0.010006 0.005839 0.013155
Bud Site 0.001002 0.005381 0.001106 0.012278 0.001428 0.006330 0.001851 0.008053 0.001187 0.005902 0.001791 0.010264
Cell Periphery 0.000100 0.000125 0.000077 0.000100 0.000122 0.000169 0.000080 0.000086 0.000109 0.000149 0.000080 0.000093
Cytoplasm 0.370832 0.252998 0.004686 0.039584 0.328801 0.234746 0.019470 0.018859 0.352625 0.242974 0.018283 0.029704
Cytoplasmic Foci 0.055134 0.054383 0.000315 0.001403 0.090779 0.074727 0.000330 0.000294 0.070575 0.065555 0.000329 0.000875
Eisosomes 0.000041 0.000049 0.000054 0.000021 0.000060 0.000069 0.000015 0.000019 0.000049 0.000060 0.000018 0.000020
Endoplasmic Reticulum 0.000350 0.001476 0.012037 0.007765 0.000976 0.001449 0.004243 0.007847 0.000621 0.001461 0.004868 0.007804
Endosome 0.001520 0.004745 0.000537 0.003007 0.003068 0.004180 0.000439 0.001020 0.002191 0.004435 0.000446 0.002060
Golgi 0.000459 0.001563 0.000117 0.002189 0.000583 0.002186 0.000030 0.000454 0.000513 0.001905 0.000037 0.001362
Lipid Particles 0.003299 0.004136 0.000791 0.000405 0.006798 0.008142 0.000199 0.000227 0.004815 0.006336 0.000247 0.000320
Mitochondria 0.002662 0.006564 0.001224 0.004095 0.003383 0.011008 0.000760 0.001331 0.002975 0.009005 0.000797 0.002778
Mitotic Spindle 0.000203 0.004309 0.008002 0.019042 0.000854 0.003710 0.001548 0.007468 0.000485 0.003980 0.002066 0.013524
None 0.007978 0.011138 0.000942 0.004217 0.006798 0.006657 0.003298 0.004628 0.007467 0.008677 0.003109 0.004413
Nuclear Periphery 0.000759 0.001347 0.001055 0.003572 0.001165 0.001654 0.001141 0.002035 0.000935 0.001516 0.001134 0.002839
Nuclear Periphery Foci 0.000209 0.000572 0.000296 0.000493 0.000430 0.000567 0.000161 0.000666 0.000305 0.000569 0.000172 0.000576
Nucleolus 0.001916 0.004932 0.002493 0.001538 0.002735 0.004865 0.000956 0.001050 0.002270 0.004895 0.001080 0.001306
Nucleus 0.539401 0.616254 0.950296 0.865920 0.529935 0.600846 0.954203 0.928933 0.535300 0.607792 0.953890 0.895961
Peroxisomes 0.001336 0.006229 0.000945 0.000270 0.002720 0.009155 0.000144 0.000198 0.001935 0.007836 0.000208 0.000236
Vacuole 0.006390 0.014291 0.010523 0.014195 0.011974 0.016408 0.004754 0.007319 0.008809 0.015454 0.005217 0.010917
Vacuole Periphery 0.000202 0.001220 0.000193 0.001982 0.000339 0.001081 0.000112 0.000187 0.000261 0.001144 0.000118 0.001126

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -3.80 -8.91 -7.55 -4.19 2.06 -4.03 -11.96 -17.58 -2.85 -9.33 -5.57 -15.17 -11.70 -4.97 -6.20
Bud Neck -1.91 1.14 -8.47 -6.98 -8.83 -3.85 -0.46 -5.53 -1.87 -4.83 -4.36 -0.75 -10.07 -6.14 -7.98
Bud Site -4.62 -1.91 -6.28 -1.96 -4.68 -4.56 -0.68 -3.68 -0.37 -3.28 -6.63 -1.41 -6.91 -1.54 -5.41
Cell Periphery -2.12 -0.29 0.22 2.56 0.38 -1.71 2.41 1.91 3.51 -1.17 -2.64 2.37 1.75 4.18 -1.40
Cytoplasm 14.58 67.52 61.12 32.18 -10.55 12.04 50.94 51.57 39.90 0.72 19.71 80.97 80.16 50.36 -4.00
Cytoplasmic Foci 0.04 23.96 23.74 18.36 -2.11 3.37 27.91 27.94 24.40 0.67 1.47 36.39 36.30 30.41 -1.23
Eisosomes -3.28 -3.77 3.19 5.68 4.89 -2.53 16.44 12.36 13.30 -5.93 -4.94 16.21 11.16 13.77 -6.64
Endoplasmic Reticulum -11.83 -10.72 -25.29 -21.94 3.30 -2.69 -14.23 -20.01 -19.79 -8.49 -8.70 -19.66 -32.40 -29.51 -9.73
Endosome -5.26 3.05 -0.37 4.54 -2.35 -1.91 5.41 4.03 6.68 -1.72 -5.45 6.76 2.70 7.58 -3.47
Golgi -2.65 1.85 -0.94 1.22 -1.86 -2.72 4.97 1.21 3.07 -1.22 -3.68 4.23 -0.32 3.03 -2.38
Lipid Particles -2.06 8.19 10.35 10.09 3.76 -1.47 12.21 12.07 10.15 -1.91 -2.97 16.15 15.74 13.09 -3.14
Mitochondria -3.35 1.94 -0.70 3.01 -2.95 -5.80 4.79 3.01 7.60 -4.52 -6.75 5.39 1.14 7.69 -5.80
Mitotic Spindle -4.52 -1.33 -6.84 -4.74 -1.89 -3.25 -1.47 -4.83 -2.96 -4.10 -5.56 -2.52 -8.40 -5.58 -6.58
None -2.36 11.78 4.51 4.92 -10.30 0.03 4.38 2.88 2.67 -1.72 -1.62 6.81 5.27 5.50 -2.89
Nuclear Periphery -5.94 -4.43 -9.62 -8.07 -7.50 -2.98 -3.79 -9.09 -6.18 -7.63 -5.91 -8.12 -12.75 -9.90 -10.26
Nuclear Periphery Foci -2.09 -0.97 -2.46 -1.76 -2.14 -1.88 2.10 -2.22 -1.82 -2.50 -2.76 1.13 -3.26 -2.50 -3.56
Nucleolus -4.11 -0.21 -2.08 1.93 -1.87 -3.26 2.86 -0.02 3.18 -2.74 -5.24 3.00 -1.67 3.50 -4.02
Nucleus -7.53 -32.63 -41.31 -25.41 6.03 -6.83 -56.73 -46.99 -37.10 7.02 -10.04 -79.55 -62.35 -43.46 13.77
Peroxisomes -6.14 1.27 5.71 7.88 1.16 -6.63 9.58 9.58 9.72 -0.40 -9.22 10.12 10.80 12.46 0.08
Vacuole -8.11 -2.96 -11.11 -5.39 -0.79 -4.05 2.51 -1.41 1.83 -3.08 -9.01 -1.24 -10.07 -3.23 -7.16
Vacuole Periphery -2.63 -0.65 -1.65 0.65 -1.53 -3.87 3.93 3.38 4.77 -1.01 -4.34 2.87 -1.29 2.12 -1.88
Protein Complexes26S Proteasome complex37S mitochondrial small ribosomal subunit54S mitochondrial large ribosomal subunit60S cytosolic large ribosomal subunitAar2-U5 snRNPAnaphase-Promoting Complex variant 1Anaphase-Promoting Complex variant 2Anaphase-Promoting Complex variant 3Anaphase-Promoting Complex variant 4Arp2/3 complexATG1 kinase complexBLOC-1 complexCcr4-Not complexCentral kinetochore CTF19 complexCleavage and polyadenylation specificity factorcomplexCMG helicase complexCMG-Pol epsilon complexCOMPASS complexCOP9 signalosome COPI vesicle coat complexCoQ6 biosynthesis complexCore mediator complexCORVET complexCTF18-RFC complexCURI complex variant 1CURI complex variant 2CURI complex variant 3Cytoplasmic exosome complex, DIS3 variantDASH complexDNA-directed RNA Polymerase I complexDNA-directed RNA polymerase II complexDNA-directed RNA polymerase III complexDNA replication factor C complexEGO complexELG1-RFC complex Elongator holoenzyme complexEndoplasmic Reticulum Membrane ComplexEukaryotic translation initiation factor 2BcomplexEukaryotic translation initiation factor 3core complexExocystExomer complexFAR complexGeneral transcription factor complex TFIIDGeneral transcription factor complex TFIIHGET4-GET5 transmembrane domain recognition complexGID ubiquitin ligase complexGin4 complexGolgi transport complexGSE complexHMC complexHOPS complexINO80 chromatin remodeling complexLSM1-7 complexLsm1-7-Pat1 complexLSM2-7 complexLSM2-8 complexMCM complexMICOS complexMitochondrial electron transport complex IIIMitochondrial inner membrane pre-sequence translocasecomplexMitochondrial outer membrane translocase holocomplexMitochondrial proton-transporting ATP synthase complexMitochondrial pyruvate dehydrogenase complexNineTeen complexNOP8 60s ribosome pre-assembly complexNuA3 histone acetyltransferase complexNuA4 histone acetyltransferase complexNuclear condensin complexNuclear/nucleolar exosome complex, DIS3-RRP6 variantNuclear pore complexNucleolar ribonuclease MRP complexNucleolar ribonuclease P complexOligosaccharyl transferase complex variant 1Oligosaccharyl transferase complex variant 2PAF1 complexPAS complexPhosphatidylinositol 3-kinase complex IPolarisomePrefoldin complexPRP19-associated complexRad17 RFC-like complexRetromer complexRPD3L histone deacetylase complexRPD3S histone deacetylase complexRQC complexRSC complex variant RSC1RSC complex variant RSC2SAGA complexSCF-Met30 ubiquitin ligase complexSCF-Ufo1 ubiquitin ligase complexSEA complexSeptin complexSET3C histone deacetylase complexSignal recognition particleSLIK (SAGA-like) complexSmall ribosomal subunit processome, variant1Small ribosomal subunit processome, variant2Small ribosomal subunit processome, variant3SMC5-SMC6 SUMO ligase complexSm complexSpliceosomal commitment complexSPOTS complexSWI/SNF chromatin remodelling complexSwr1 chromatin remodelling complexTHO complexTranscription factor TFIIIC complexTranslocon complexTRAPPIII protein complexTRAPPII protein complexTRAPPI protein complexTREX complexU1 snRNPU2 snRNPU4 snRNPU4/U6 snRNPU4/U6.U5 tri-snRNP complexU5 snRNPU6 snRNP complexUTP-A complexUTP-B complexUTP-C complex variant 1UTP-C complex variant 2UTP-C complex variant 3Vacuolar proton translocating ATPase complex,Golgi variantVacuolar proton translocating ATPase complex,vacuole variantCellular Components••••••••••••••••actinbud neckcell peripherycytoplasmendosomeERGolgimitochondrionmixednuclear peripherynucleolusnucleusperoxisomespindle polevacuolar membranevacuole
Standard name
Human Ortholog
Description DNA replication initiation protein; loads DNA pol epsilon onto pre-replication complexes at origins; checkpoint sensor recruited to stalled replication forks by the checkpoint clamp complex where it activates Mec1p; along with Rfa1p, binds to ultrafine anaphase bridges in mitotic cells and prevents accumulation of chromatin bridges by stimulating the Mec1p kinase and suppressing homologous recombination; ortholog of human TopBP1; forms nuclear foci upon DNA replication stress
Localization
Cell Percentages nucleus (27%), cytoplasm (8%), mixed (50%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Dpb11

Dpb11


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Dpb11-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available