Standard name
Human Ortholog
Description DNA helicase and DNA-dependent ATPase; role in DNA repair and checkpoint recovery, in the proper timing of commitment to meiotic recombination and the Meiosis I to II transition; blocks trinucleotide repeat expansion; affects genome stability; disassembles Rad51p nucleoprotein filaments during meiotic recombination; stimulates Mus81p-Mms4p endonuclease activity independent of catalytic activity; ATPase and ssDNA translocating motor activities inhibited by Dmc1p; functional homolog of human RTEL1
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0.05 0.05 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.05 0.08 0.1 0.13 0.09 0.09 0.07 0.06 0.1 0.05 0.05 0.06 0.14 0.23 0.31 0.06 0.12 0.12 0 0 0 0.07 0.07 0.09
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0.3 0.55 0.65 0.38 0.61 0.61 0.62 0.74 0.76 0.77 0.81 0.07 0 0.21 0 0 0.05 0 0 0 0 0 0
Nucleus 0.9 0.67 0.58 0.59 0.72 0.72 0.72 0.71 0.63 0.65 0.66 0.62 0.66 0.58 0.43 0.86 0.82 0.78 0.79 0.74 0.6 0.7 0.46 0.35
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.08 0.11 0 0 0 0 0 0 0 0 0 0 0.21 0.2 0.16 0.1 0.07 0.09 0.13 0.13 0.27 0.13 0.33 0.38
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 1 0 1 0
Bud 0 0 3 3 1 3 5 11 5 6 7 7 0 2 1 1 1 1 4 1 2 2 4 2
Bud Neck 0 0 1 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1
Bud Site 0 1 1 3 3 14 28 30 16 10 22 31 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0
Cytoplasm 19 8 23 40 35 38 45 45 41 27 21 35 15 31 51 20 34 29 8 4 8 4 11 15
Endoplasmic Reticulum 0 0 1 0 0 0 0 0 0 1 0 0 2 0 0 3 1 4 0 0 1 0 0 2
Endosome 2 1 0 0 2 0 1 0 0 0 0 0 3 7 3 3 4 2 0 2 0 0 1 3
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 0 1 1 0 0 0 0 1 0
Mitochondria 8 30 127 201 142 272 396 455 295 398 343 513 7 3 34 7 6 12 3 1 6 0 3 6
Nucleus 332 66 135 182 266 317 465 520 253 338 297 392 70 79 71 275 241 185 313 82 152 43 75 58
Nuclear Periphery 0 1 0 0 1 1 5 11 3 7 5 8 0 0 1 0 1 1 0 0 0 0 0 0
Nucleolus 0 0 0 0 1 5 7 13 7 6 6 12 0 0 0 0 2 2 0 0 0 0 2 4
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 1 0 0 0 0 1 5 3 1 1 0 2 0 0 0 1 1 0 7 2 4 1 6 4
Vac/Vac Membrane 28 11 8 7 9 8 5 8 7 4 7 24 22 27 26 32 20 21 53 14 67 7 54 63
Unique Cell Count 367 99 231 311 371 443 645 734 399 524 447 630 106 136 164 321 295 237 396 112 253 62 166 167
Labelled Cell Count 390 118 299 436 460 659 964 1096 628 798 708 1024 119 150 188 343 313 258 396 112 253 62 166 167


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.4 4.0 3.5 3.5 3.8 3.6 3.4 3.5 3.2 3.1 3.2 2.9 5.0 5.2 4.6 7.2 7.6 8.2 4.6 5.0 5.3
Std Deviation (1e-4) 0.7 0.9 0.8 1.2 1.1 1.2 1.1 1.1 1.3 0.7 1.2 1.0 1.3 1.4 1.7 1.6 1.6 1.7 0.7 1.8 1.2
Intensity Change (Log2) – – – 0.01 0.14 0.03 -0.03 0.02 -0.13 -0.19 -0.12 -0.27 0.53 0.58 0.39 1.05 1.12 1.24 0.42 0.51 0.61


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – 0 0 0
Bud Neck – – – – – – – – – – – – 0 0 0
Bud Site – – – – – – – – – – – – 0 0 0
Cell Periphery – – – – – – – – – – – – 0 0 0
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – 0 0 0
Endosome – – – – – – – – – – – – 0 0 0
Golgi – – – – – – – – – – – – 0 0 0
Mitochondria – – – – – – – – – – – – 0 0 0
Nuclear Periphery – – – – – – – – – – – – 0 0 0
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – 0 0 0
Peroxisomes – – – – – – – – – – – – 0 0 0
SpindlePole – – – – – – – – – – – – 0 0 0
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – 0 0 0
Cytoplasm – – – – – – – – – – – – 0 0 0
Nucleus – – – – – – – – – – – – 0 0 0
Vacuole – – – – – – – – – – – – 2.9 1.7 2.4

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 0.6504 1.5635 1.3678 1.5215 1.4779 1.3224 3.4022 3.1237 3.0716 3.0645 2.8821 3.2624 1.3524 0.8107 1.5813 1.5906 1.692 1.4981
Actin 0.0001 0.0001 0 0 0 0.0001 0.0346 0.0006 0.0043 0.0252 0.0051 0.0093 0.0135 0.0001 0.0072 0.0002 0.0033 0.0004
Bud 0 0.0001 0 0 0 0 0.0011 0.0003 0.0003 0.0008 0.0005 0.0003 0.0008 0 0.0001 0 0.0001 0.0001
Bud Neck 0.0002 0.0003 0.0001 0.0001 0.0002 0.0018 0.0092 0.001 0.0028 0.0011 0.0003 0.0012 0.0032 0.0001 0.0002 0.0004 0.0011 0.0015
Bud Periphery 0 0.0001 0 0 0 0 0.0025 0.0002 0.0007 0.002 0.0011 0.0006 0.0004 0.0001 0.0003 0 0.0002 0.0001
Bud Site 0.0002 0.0003 0 0 0.0001 0.0001 0.0077 0.0069 0.0027 0.0015 0.0012 0.0003 0.0013 0.0002 0.0004 0 0.0004 0.0001
Cell Periphery 0 0 0 0 0 0 0.0009 0.0001 0.0003 0.0003 0.0001 0 0.0001 0 0.0001 0 0 0
Cytoplasm 0.0128 0.0072 0.0011 0.0005 0.0004 0.0011 0.014 0.0262 0.0043 0.0007 0.0003 0.0009 0.0181 0.0073 0.0046 0.0126 0.0045 0.0027
Cytoplasmic Foci 0.0003 0.0001 0 0 0 0.0003 0.0178 0.0021 0.0062 0.0057 0.0037 0.0037 0.006 0.0059 0.0053 0.0003 0.007 0.0001
Eisosomes 0 0 0 0 0 0 0.0003 0 0.0001 0.0002 0 0 0.0002 0.0003 0.0001 0 0 0
Endoplasmic Reticulum 0.0008 0.0017 0 0 0 0.0001 0.0074 0.001 0.0011 0.0008 0.0004 0.0008 0.0028 0.0003 0.0008 0.0002 0.0002 0.0002
Endosome 0.0008 0.0008 0 0 0 0.0003 0.036 0.0038 0.0093 0.028 0.007 0.0084 0.007 0.0089 0.0051 0.0003 0.0023 0.0003
Golgi 0 0 0 0 0 0 0.0173 0.0017 0.0034 0.0235 0.0053 0.0069 0.0018 0.0001 0.0013 0 0.0015 0
Lipid Particles 0.0001 0 0 0 0 0.0009 0.0288 0.0005 0.0076 0.0301 0.0063 0.004 0.0072 0.0007 0.0089 0 0.0038 0
Mitochondria 0.0003 0.0004 0.0001 0.0001 0.0002 0.0001 0.0363 0.0012 0.0039 0.0466 0.0141 0.0111 0.0068 0.0004 0.0074 0.0002 0.0005 0.0005
None 0.0272 0.0012 0.0006 0 0.0001 0.0031 0.0215 0.0108 0.0034 0.0005 0.0002 0.0003 0.0528 0.3219 0.0106 0.0075 0.014 0.006
Nuclear Periphery 0.0092 0.0193 0.0028 0.0014 0.0013 0.0033 0.029 0.0066 0.0122 0.0043 0.0116 0.0083 0.0143 0.0056 0.017 0.0023 0.0022 0.0041
Nucleolus 0.0073 0.0071 0.0044 0.0046 0.0104 0.0124 0.0152 0.0049 0.0043 0.0049 0.0053 0.0127 0.0114 0.0058 0.0039 0.0048 0.0155 0.0149
Nucleus 0.9327 0.9559 0.9896 0.9928 0.9864 0.971 0.6644 0.9155 0.9191 0.7518 0.9231 0.9212 0.8054 0.6199 0.9095 0.9363 0.9023 0.9614
Peroxisomes 0 0 0 0 0 0 0.0173 0.0055 0.0079 0.0055 0.0084 0.0033 0.0071 0.0003 0.0068 0 0.0133 0
Punctate Nuclear 0.007 0.0033 0.0011 0.0002 0.0005 0.0049 0.0215 0.0089 0.0023 0.0606 0.0049 0.0054 0.0378 0.0208 0.0086 0.0346 0.0269 0.0071
Vacuole 0.0007 0.0014 0.0001 0.0001 0.0003 0.0003 0.013 0.0019 0.0027 0.0035 0.0005 0.0005 0.0012 0.0009 0.001 0.0001 0.0005 0.0002
Vacuole Periphery 0.0001 0.0005 0 0 0.0001 0.0001 0.004 0.0003 0.0012 0.0025 0.0006 0.0006 0.0006 0.0002 0.0006 0 0.0001 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 28.172 26.863 10.5897 12.5426 27.5976 14.7039 21.927 19.0003 14.8521 24.5134
Translational Efficiency 0.3788 0.3752 0.3454 0.2928 0.259 0.5308 0.3745 0.2802 0.3113 0.3156

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
649 211 922 1120 1284 1890 1371 1900 1933 2101 2293 3020

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 713.38 750.40 911.43 882.92 582.13 738.95 877.22 909.17 626.20 740.10 890.98 899.43
Standard Deviation 89.99 92.29 111.65 113.73 90.44 87.26 103.14 132.88 109.52 87.85 107.95 126.75
Intensity Change Log 2 — 0.072989 0.353461 0.307612 — 0.344135 0.591597 0.643209 — 0.201163 0.465351 0.468123

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000028 0.000146 0.000315 0.000860 0.000079 0.000137 0.000176 0.000446 0.000062 0.000138 0.000232 0.000600
Bud Neck 0.000359 0.003530 0.004939 0.013094 0.003540 0.008665 0.004246 0.014576 0.002472 0.008149 0.004524 0.014026
Bud Site 0.000219 0.001932 0.003663 0.006781 0.001469 0.003363 0.000908 0.004292 0.001050 0.003219 0.002015 0.005215
Cell Periphery 0.000104 0.000159 0.000103 0.000145 0.000264 0.000175 0.000115 0.000370 0.000210 0.000174 0.000110 0.000286
Cytoplasm 0.392239* 0.075313 0.023461 0.021053 0.143100* 0.104292* 0.016191 0.023282 0.226748* 0.101382* 0.019114 0.022455
Cytoplasmic Foci 0.030764 0.016365 0.000788 0.002140 0.010872 0.020913 0.000333 0.000823 0.017550 0.020457 0.000516 0.001311
Eisosomes 0.000034 0.000039 0.000017 0.000025 0.000026 0.000042 0.000015 0.001225 0.000029 0.000042 0.000016 0.000780
Endoplasmic Reticulum 0.000328 0.001982 0.004146 0.008933 0.001306 0.001724 0.003249 0.004930 0.000978 0.001750 0.003610 0.006415
Endosome 0.000829 0.003463 0.000414 0.002262 0.001229 0.003335 0.000241 0.000878 0.001095 0.003348 0.000311 0.001391
Golgi 0.000059 0.000269 0.000042 0.002729 0.000260 0.001724 0.000023 0.000763 0.000192 0.001578 0.000030 0.001493
Lipid Particles 0.001096 0.001725 0.000309 0.000736 0.001521 0.002941 0.000169 0.001019 0.001379 0.002819 0.000225 0.000914
Mitochondria 0.000155 0.006702 0.000511 0.003817 0.001236 0.006083 0.000432 0.003102 0.000873 0.006145 0.000463 0.003367
Mitotic Spindle 0.000004 0.004770 0.000656 0.015567 0.000968 0.004893 0.000603 0.006482 0.000644 0.004881 0.000624 0.009851
None 0.016862 0.007151 0.003233 0.003567 0.060244 0.007535 0.004567 0.008203 0.045679 0.007497 0.004030 0.006483
Nuclear Periphery 0.000229 0.000694 0.000670 0.002529 0.001110 0.001447 0.000602 0.002478 0.000814 0.001372 0.000630 0.002497
Nuclear Periphery Foci 0.000362 0.000330 0.000218 0.001028 0.000580 0.000274 0.000069 0.000620 0.000507 0.000279 0.000129 0.000771
Nucleolus 0.001123 0.003104 0.001315 0.001453 0.006622 0.004432 0.001073 0.000688 0.004776 0.004299 0.001170 0.000972
Nucleus 0.547919* 0.862283* 0.951859* 0.902267* 0.762175* 0.812607* 0.964633* 0.914282* 0.690239* 0.817596* 0.959497* 0.909826*
Peroxisomes 0.000309 0.001856 0.000087 0.000364 0.000582 0.003025 0.000048 0.003022 0.000490 0.002908 0.000064 0.002036
Vacuole 0.006905 0.007721 0.003163 0.009883 0.002620 0.010858 0.002250 0.007344 0.004059 0.010543 0.002617 0.008286
Vacuole Periphery 0.000074 0.000467 0.000093 0.000768 0.000197 0.001533 0.000057 0.001176 0.000156 0.001426 0.000071 0.001024

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -5.95 -10.89 -9.01 -7.72 -5.61 -1.71 -9.68 -5.48 -4.24 -3.58 -2.50 -14.05 -9.99 -7.81 -6.30
Bud Neck -3.23 -11.93 -12.49 -8.18 -8.46 -4.91 -1.68 -13.59 -6.92 -9.72 -6.21 -4.30 -18.07 -8.12 -12.88
Bud Site -1.69 -2.50 -5.80 -3.49 -2.17 -2.52 1.37 -3.81 -1.06 -4.36 -3.25 -1.75 -6.71 -2.65 -4.06
Cell Periphery -1.55 0.06 -1.42 0.69 -1.21 2.69 4.87 0.38 -0.96 -1.92 1.51 4.79 0.27 -0.60 -2.14
Cytoplasm 21.96 32.52 32.93 5.71 0.22 5.68 21.18 20.26 20.61 -3.02 18.10 33.90 33.61 21.65 -2.00
Cytoplasmic Foci 3.26 13.73 13.34 4.37 -0.61 -4.81 9.20 8.61 12.88 -1.40 -1.58 15.65 14.99 13.48 -1.33
Eisosomes -0.96 5.52 -0.45 0.79 -7.41 -1.54 4.12 -2.59 -2.52 -2.63 -1.40 6.27 -2.59 -2.50 -2.67
Endoplasmic Reticulum -7.36 -16.85 -16.86 -13.20 -8.82 -2.36 -10.06 -16.91 -13.43 -5.85 -4.89 -17.48 -23.08 -18.28 -10.33
Endosome -1.52 2.88 -2.39 1.01 -3.69 -3.47 3.36 1.61 5.00 -3.95 -4.18 3.83 0.17 4.45 -5.18
Golgi -1.60 0.98 -2.10 -1.73 -2.13 -2.42 2.41 -0.53 2.10 -2.09 -2.62 2.45 -2.00 1.33 -2.88
Lipid Particles -1.61 5.08 1.89 2.46 -1.48 -2.76 6.10 3.21 4.75 -2.60 -3.25 7.25 3.69 5.04 -2.91
Mitochondria -1.34 -11.76 -3.54 0.68 -3.08 -4.87 2.22 -2.86 3.22 -5.49 -5.30 1.61 -4.38 3.02 -5.86
Mitotic Spindle -1.28 -1.34 -6.89 -3.78 -6.22 -3.37 -0.54 -4.71 -1.44 -4.09 -3.90 -1.18 -8.28 -4.64 -7.40
None 4.12 7.40 6.17 1.37 -2.37 11.97 12.74 12.07 0.86 -1.48 12.43 13.87 12.99 1.59 -2.26
Nuclear Periphery -6.31 -11.23 -7.54 -6.44 -6.31 -1.26 1.47 -5.10 -5.04 -7.46 -2.85 0.38 -8.15 -6.96 -9.77
Nuclear Periphery Foci 0.19 0.49 -2.84 -2.86 -2.92 3.17 5.99 -0.97 -1.78 -2.29 2.87 4.91 -2.70 -3.35 -3.68
Nucleolus -2.65 -0.28 -2.95 0.53 -3.14 2.63 9.49 4.29 1.86 -3.01 0.63 8.77 3.32 2.33 -3.96
Nucleus -16.24 -32.34 -23.67 -0.60 10.11 -4.83 -22.93 -15.77 -13.22 10.82 -13.71 -35.52 -25.35 -11.63 14.63
Peroxisomes -2.18 1.39 0.43 2.38 -2.45 -4.10 2.54 -1.08 1.80 -2.46 -5.02 2.82 -0.95 3.18 -2.61
Vacuole -0.45 3.07 -6.49 -4.53 -7.78 -7.70 -0.44 -7.68 0.58 -6.94 -6.47 1.38 -9.88 -2.84 -10.31
Vacuole Periphery -1.77 -2.04 -2.21 0.00 -2.08 -3.30 6.23 -1.27 1.30 -1.59 -3.48 5.37 -1.74 1.68 -2.03
Standard name
Human Ortholog
Description DNA helicase and DNA-dependent ATPase; role in DNA repair and checkpoint recovery, in the proper timing of commitment to meiotic recombination and the Meiosis I to II transition; blocks trinucleotide repeat expansion; affects genome stability; disassembles Rad51p nucleoprotein filaments during meiotic recombination; stimulates Mus81p-Mms4p endonuclease activity independent of catalytic activity; ATPase and ssDNA translocating motor activities inhibited by Dmc1p; functional homolog of human RTEL1
Localization
Cell Percentages nucleus (60%), cytoplasm (10%), mixed (28%)
Cell Cycle Regulation Yes
cytoplasm - MA (1.7e-05)
Subcompartmental Group N/A

Srs2

Srs2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Srs2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available