Standard name
Human Ortholog
Description GATA zinc finger protein; negatively regulates nitrogen catabolic gene expression by competing with Gat1p for GATA site binding; function requires a repressive carbon source; dimerizes with Dal80p and binds to Tor1p; GZF3 has a paralog, DAL80, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0.09 0.09 0.1 0.15 0.08 0.09 0.08 0.09 0.13 0.18 0.25 0.21 0.16 0.15 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.19 0 0.08 0.21 0.31 0.71 0.66 0.64 0.77 0.79 0.8 0.82 0 0 0 0.07 0 0.06 0 0 0 0 0 0
Nucleus 0.92 0.95 0.9 0.87 0.63 0.45 0.47 0.42 0.43 0.38 0.37 0.37 0.82 0.75 0.67 0.69 0.76 0.74 0.85 0.85 0.79 0.71 0.61 0.56
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.07 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.1 0.11 0.07
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.05
Vac/Vac Membrane 0 0 0 0 0.07 0.08 0.05 0 0 0 0.05 0.05 0 0.07 0.07 0.05 0.06 0.08 0.06 0.06 0.1 0.11 0.1 0.18
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 2 0 0 1
Bud 0 0 1 0 4 1 10 6 7 17 7 9 0 0 0 0 0 1 3 4 4 1 4 8
Bud Neck 0 0 0 0 0 0 0 1 0 0 2 0 0 0 0 0 0 0 1 0 0 0 2 6
Bud Site 3 0 0 2 1 8 14 9 13 30 5 15 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 1 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 1 0 0 0
Cytoplasm 10 5 15 9 33 37 58 99 49 62 25 38 52 75 117 88 43 30 0 0 3 0 1 1
Endoplasmic Reticulum 0 0 2 0 1 1 0 1 2 1 0 0 0 0 0 14 10 4 0 0 1 0 1 2
Endosome 1 3 3 0 16 4 4 5 0 1 0 0 2 2 11 16 6 5 3 3 7 2 12 13
Golgi 0 1 2 0 1 0 0 0 0 0 0 0 1 0 0 1 1 0 3 2 0 1 2 2
Mitochondria 105 8 36 53 108 296 368 429 444 556 240 359 6 1 4 28 2 11 2 4 3 3 5 7
Nucleus 503 414 412 220 221 187 262 285 249 266 111 161 321 317 315 282 208 146 471 390 387 141 199 226
Nuclear Periphery 16 0 6 3 0 1 1 4 5 0 1 3 0 1 0 0 0 1 2 0 1 0 2 3
Nucleolus 41 6 10 10 5 2 8 9 7 15 5 16 6 1 0 2 0 1 16 17 18 19 36 30
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 1 2 0 0 0 0 0 0 0 0 0 0
SpindlePole 2 0 2 3 0 0 3 8 3 8 1 3 4 2 0 9 4 6 6 4 7 3 16 20
Vac/Vac Membrane 5 10 8 2 25 32 29 25 21 24 14 21 9 29 32 22 17 15 35 29 46 22 32 71
Unique Cell Count 549 435 459 252 349 419 556 673 579 700 301 439 392 420 471 411 274 197 557 461 490 200 326 404
Labelled Cell Count 686 448 497 302 415 569 757 881 800 980 412 625 402 430 479 462 291 220 557 461 490 200 326 404


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.8 6.3 5.5 4.7 4.4 3.6 3.3 3.5 3.4 2.9 3.4 3.1 6.1 5.2 5.1 6.8 7.6 7.2 5.2 5.4 5.8
Std Deviation (1e-4) 0.8 1.2 1.3 1.4 1.9 1.1 1.4 1.2 0.8 0.8 1.6 0.9 1.3 1.2 1.4 1.7 1.8 1.6 1.1 1.5 1.3
Intensity Change (Log2) – – – -0.21 -0.31 -0.6 -0.71 -0.63 -0.71 -0.9 -0.71 -0.8 0.14 -0.07 -0.1 0.31 0.47 0.39 -0.07 -0.04 0.09


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.2 3.7 3.5 4.4 6.3 3.5 3.7 3.0 3.4 5.4 7.1 9.4 8.3 6.0 5.6
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 3.3 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 2.1 3.3 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 -4.2 -5.6 -5.3 -0.6 -4.2 -1.0
Nucleus -1.0 -9.0 -14.3 -14.3 -16.1 -15.6 -17.5 -15.4 -16.5 -3.3 -5.6 -8.4 -7.8 -5.0 -5.2
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 -0.7 -2.6 0 -2.1 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 2.3 0 0
Vacuole 0 3.9 4.2 2.9 1.9 1.8 1.7 2.3 2.6 0.6 3.8 3.8 2.9 3.2 3.7

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.6681 1.3679 1.4214 1.7643 1.4332 1.467 4.454 4.1414 4.134 3.7623 3.7154 4.1927 5.2297 5.0402 5.2863 4.7409 5.0884 5.4352
Actin 0.0089 0.0027 0.0072 0.0002 0 0.0018 0.0188 0.0019 0.0061 0.0135 0.0203 0.005 0 0 0 0.0001 0 0
Bud 0.0003 0.0001 0 0.0001 0 0.0002 0.0002 0.0001 0.0002 0 0.0001 0 0 0 0 0 0 0
Bud Neck 0.0004 0.0008 0.0002 0.0002 0.0009 0.002 0.0046 0.0002 0.0006 0.0002 0.0007 0.0006 0 0.0004 0 0.0001 0.0009 0.0003
Bud Periphery 0.0013 0.0001 0 0.0001 0 0.001 0.0005 0.0001 0.0004 0 0.0003 0 0 0 0 0 0 0
Bud Site 0.0007 0.0031 0.0002 0.0003 0.0001 0.0003 0.0017 0.0001 0.0013 0.0002 0.0002 0 0 0.0001 0 0 0.0001 0
Cell Periphery 0.0003 0.0002 0 0 0 0.0004 0.0002 0 0.0001 0 0.0001 0 0 0 0 0 0 0
Cytoplasm 0.0003 0.0102 0.0015 0.0012 0.0007 0.0002 0.0016 0.0007 0.0018 0.0012 0.0006 0.0007 0.0001 0.0003 0.0001 0.0002 0.0002 0.0001
Cytoplasmic Foci 0.0027 0.0186 0.0014 0.1032 0 0.0001 0.0066 0.0024 0.002 0.0093 0.0012 0.0007 0 0 0 0 0 0
Eisosomes 0.0003 0.0001 0 0 0 0.0001 0.0003 0 0.0001 0 0.0003 0 0 0 0 0 0 0
Endoplasmic Reticulum 0.0025 0.0079 0.0001 0.0001 0.0001 0.0004 0.003 0.0001 0.0002 0.0003 0.0008 0.0006 0 0 0 0 0 0
Endosome 0.0151 0.0101 0.0002 0.005 0 0.0011 0.0084 0.0009 0.0009 0.0025 0.006 0.0019 0 0.0001 0 0 0.0001 0
Golgi 0.0117 0.0016 0.0006 0.0015 0 0.0003 0.0048 0.0017 0.0006 0.0012 0.0056 0.0003 0 0 0 0 0 0
Lipid Particles 0.0708 0.0117 0.0031 0.0103 0 0.0002 0.0076 0.0007 0.0022 0.0014 0.0059 0.0007 0 0 0 0 0 0
Mitochondria 0.0507 0.0003 0.0004 0.0004 0.0005 0.0131 0.0021 0.0024 0.0043 0.0001 0.0069 0.0014 0 0.0001 0 0.0001 0.0001 0
None 0.0014 0.0065 0.0098 0.0006 0.0001 0.0001 0.0018 0.0007 0.0072 0.0032 0.0008 0.0035 0 0.0001 0 0.0001 0.0001 0
Nuclear Periphery 0.059 0.0102 0.0033 0.0023 0.0029 0.006 0.0145 0.0031 0.0027 0.0015 0.0095 0.0023 0.0005 0.0013 0.0005 0.0067 0.0011 0.0003
Nucleolus 0.042 0.0119 0.0174 0.0105 0.0123 0.0321 0.0188 0.0163 0.0165 0.0043 0.0305 0.0349 0.0089 0.0133 0.0096 0.0106 0.043 0.023
Nucleus 0.7059 0.8787 0.933 0.843 0.9814 0.9316 0.8443 0.9197 0.9298 0.9448 0.8922 0.9225 0.9892 0.9737 0.9876 0.9685 0.9448 0.9708
Peroxisomes 0.0035 0.0005 0.0143 0.0079 0 0.0001 0.0034 0.0003 0.0015 0.003 0.0016 0.0006 0 0 0 0 0 0
Punctate Nuclear 0.0027 0.023 0.0071 0.0128 0.0005 0.0037 0.0548 0.0484 0.0207 0.013 0.0149 0.024 0.0012 0.0105 0.0021 0.0135 0.0095 0.0054
Vacuole 0.003 0.0013 0.0001 0.0002 0.0002 0.0041 0.0011 0.0001 0.0003 0 0.0003 0.0001 0 0.0001 0 0 0.0001 0
Vacuole Periphery 0.0166 0.0003 0.0001 0.0001 0.0001 0.0009 0.0009 0.0001 0.0004 0 0.0011 0.0001 0 0 0 0 0 0

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 16.9908 11.9128 14.5856 20.0238 18.5738 15.9825 18.3686 14.0235 14.2159 21.6088
Translational Efficiency 1.1589 1.6276 1.3127 0.9084 1.0138 1.2667 0.9409 1.1891 1.2709 0.9431

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description GATA zinc finger protein; negatively regulates nitrogen catabolic gene expression by competing with Gat1p for GATA site binding; function requires a repressive carbon source; dimerizes with Dal80p and binds to Tor1p; GZF3 has a paralog, DAL80, that arose from the whole genome duplication
Localization
Cell Percentages nucleus (99%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-4

Gzf3

Gzf3


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Gzf3-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available