Standard name
Human Ortholog
Description Bifunctional carbamoylphosphate synthetase/aspartate transcarbamylase; catalyzes the first two enzymatic steps in the de novo biosynthesis of pyrimidines; both activities are subject to feedback inhibition by UTP
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0.05 0.06 0 0.1 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0.05 0.06 0.06 0.09 0.1 0.1 0.1 0.15 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 1.0 0.99 0.98 0.99 0.93 0.95 0.89 0.82 0.83 0.77 0.79 0.73 0.99 0.98 0.99 0.98 0.96 0.99 0.98 0.95 0.96 0.94 0.94 0.92
Endoplasmic Reticulum 0 0 0 0 0 0 0.08 0.1 0.07 0.11 0.09 0.08 0 0 0 0 0.06 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 2 3 14 20 27 9 31 0 1 0 0 0 0 0 1 0 0 0 0
Bud 0 2 2 2 3 0 0 0 4 2 0 1 0 0 0 1 0 0 1 1 0 0 0 2
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 4 12 5 19 18 24 36 40 43 23 49 2 2 1 5 9 2 0 0 0 0 0 0
Cytoplasm 258 345 403 317 323 306 355 344 335 339 191 238 138 177 185 250 201 247 242 333 396 77 187 196
Endoplasmic Reticulum 1 2 9 1 15 11 31 44 28 50 22 27 1 2 1 10 13 10 0 3 4 0 4 4
Endosome 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 1 0 0 2 0 0 2 0 0 0 0 1 0 1 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 7 10 14 13 12 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 1 0 0 1 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 1
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 1
Nucleolus 0 0 0 0 0 0 0 1 1 0 3 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 2 0 2 2 5 2 2 8 0 1 1 1 0 3 0 6 4 0 0 1
Unique Cell Count 258 349 410 321 347 321 401 420 403 438 242 325 139 180 186 256 210 249 247 351 412 83 200 213
Labelled Cell Count 259 353 426 325 364 337 415 451 443 478 265 366 141 183 188 268 223 263 247 351 412 83 200 213


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 128.7 80.0 73.4 64.6 54.7 47.8 44.2 38.5 36.8 34.0 31.9 31.4 97.4 91.6 78.5 135.4 137.8 131.8 139.6 158.3 137.7
Std Deviation (1e-4) 31.6 21.2 17.7 13.9 15.9 11.7 11.8 10.1 9.6 8.8 9.3 7.8 21.2 21.0 18.2 50.2 51.1 49.2 40.6 41.8 42.5
Intensity Change (Log2) – – – -0.18 -0.42 -0.62 -0.73 -0.93 -0.99 -1.11 -1.2 -1.22 0.41 0.32 0.1 0.88 0.91 0.84 0.93 1.11 0.91


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 3.7 4.6 5.1 0 6.4 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery -1.2 1.8 1.8 2.1 3.5 4.1 4.1 3.6 5.9 0 0 0 -0.8 0.9 -1.8
Cytoplasm 0.5 -3.6 -2.3 -5.6 -7.9 -7.5 -9.2 -8.4 -10.1 0.8 0 1.1 -0.6 -1.9 1.0
Endoplasmic Reticulum 0 1.7 1.0 3.6 4.9 3.3 5.3 4.0 3.8 0 0 0 1.3 2.5 1.4
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 3.7 0 3.9 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 95.6918 134.2757 124.0292 135.0129 112.2588 132.6041 92.853 128.5473 124.0284 115.0633 100.5563 129.0351 116.9913 140.0868 129.6387 117.7763 105.5942 121.2015
Actin 0.0154 0.0003 0.0033 0.0004 0.001 0.001 0.0054 0.0001 0.0045 0 0.0165 0.0193 0.0163 0.0002 0.0078 0.0084 0.0003 0.0002
Bud 0.0021 0.0005 0.0027 0.0024 0.0073 0.0013 0.0011 0.0001 0.0001 0.0002 0.001 0.0001 0.0005 0.0002 0.0005 0.0009 0.0008 0.0003
Bud Neck 0.0009 0.0005 0.0019 0.0012 0.001 0.004 0.0005 0.0001 0.0006 0.0002 0.0007 0.0005 0.0003 0.0001 0.0003 0.0008 0.0003 0.0007
Bud Periphery 0.0026 0.0002 0.0039 0.0007 0.0042 0.0007 0.001 0 0.0001 0 0.0017 0.0001 0.0003 0.0001 0.0002 0.0009 0.0004 0.0001
Bud Site 0.0016 0.0002 0.0012 0.0003 0.0016 0.0002 0.0009 0.0001 0.0007 0 0.0008 0.0002 0.0004 0.0001 0.0005 0.0014 0.0001 0
Cell Periphery 0.0058 0.0013 0.0108 0.0048 0.0032 0.0099 0.0113 0.0026 0.0014 0.0002 0.0047 0 0.0009 0.0013 0.0007 0.0013 0.0012 0.0002
Cytoplasm 0.6678 0.977 0.91 0.9163 0.7952 0.7813 0.846 0.9897 0.973 0.9803 0.8609 0.9642 0.9199 0.9946 0.9524 0.8843 0.8678 0.9464
Cytoplasmic Foci 0.0021 0.0005 0.0005 0.0004 0.002 0.0009 0.012 0.0002 0.0017 0.0003 0.0072 0.001 0.0054 0.0002 0.0047 0.012 0.0144 0.0013
Eisosomes 0.0005 0 0.0009 0 0 0 0.0009 0 0.003 0 0.0007 0.0001 0.0004 0 0.0003 0.0004 0.0002 0
Endoplasmic Reticulum 0.0068 0.0006 0.0051 0.0004 0.0013 0.0035 0.0049 0.0007 0.0013 0.0005 0.0034 0.0048 0.0033 0.0005 0.001 0.0023 0.0031 0.0119
Endosome 0.0067 0.0001 0.0017 0.0004 0.0045 0.0041 0.0076 0.0001 0.0006 0.0002 0.0093 0.0012 0.0023 0.0001 0.0005 0.0041 0.0015 0.0037
Golgi 0.0011 0 0.0003 0 0.0004 0.0002 0.002 0 0.001 0 0.0058 0.002 0.0026 0 0.0003 0.0021 0.0002 0.0002
Lipid Particles 0.0013 0.0001 0.0005 0.0001 0.0004 0.0002 0.008 0.0004 0.002 0 0.0126 0.0005 0.0041 0 0.0017 0.0132 0.0036 0.0001
Mitochondria 0.054 0.0057 0.0084 0.0007 0.0068 0.0018 0.0039 0.0001 0.0012 0.0001 0.011 0.0013 0.0042 0 0.004 0.0153 0.0346 0.0005
None 0.0058 0.0006 0.0009 0.0003 0.0007 0.0004 0.0107 0.0002 0.0016 0.0002 0.0084 0.0001 0.0053 0.0001 0.0082 0.0046 0.0172 0.0005
Nuclear Periphery 0.0802 0.002 0.0133 0.0106 0.0081 0.0502 0.0158 0.0008 0.0014 0.002 0.0121 0.0015 0.0094 0.0004 0.0023 0.0035 0.0087 0.0063
Nucleolus 0.0085 0.002 0.0031 0.0014 0.0019 0.0025 0.0168 0.0001 0.0005 0.0003 0.0065 0 0.0065 0 0.0039 0.0035 0.0022 0.0004
Nucleus 0.059 0.0032 0.0095 0.0179 0.0139 0.0379 0.0151 0.001 0.0015 0.0043 0.0051 0.0013 0.0075 0.0006 0.0033 0.011 0.0068 0.0144
Peroxisomes 0.0007 0.0002 0.0001 0.0001 0.0003 0.0001 0.0018 0 0.0014 0 0.0018 0.0004 0.0019 0 0.001 0.0027 0.0043 0
Punctate Nuclear 0.0056 0.0001 0.0001 0.0001 0.0001 0.0002 0.0061 0 0.0002 0 0.0026 0.0001 0.001 0 0.0005 0.001 0.0001 0.0002
Vacuole 0.0426 0.0043 0.0157 0.0285 0.083 0.0547 0.0226 0.0036 0.0018 0.0088 0.0201 0.0008 0.0055 0.0014 0.0037 0.0146 0.0234 0.006
Vacuole Periphery 0.0286 0.0006 0.006 0.0131 0.0631 0.0447 0.0057 0.0001 0.0003 0.0021 0.007 0.0002 0.0022 0.0001 0.0023 0.0115 0.0088 0.0063

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 239.3616 215.0879 251.6883 277.5523 223.2573 199.648 225.2421 221.7417 223.008 260.4632
Translational Efficiency 0.9333 1.0309 0.9649 0.8624 0.9816 1.0167 0.8856 0.7285 0.7867 0.8262

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✔
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
2009 1084 378 1075 1804 1490 492 2994 3813 2574 870 4069

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 4666.04 5089.42 3928.91 3221.97 4702.50 5663.53 4416.27 4066.08 4683.29 5421.75 4204.52 3843.07
Standard Deviation 1164.18 1340.71 979.56 825.18 1248.92 1563.93 1378.55 874.91 1205.16 1501.06 1244.98 938.96
Intensity Change Log 2 — 0.125303 -0.248070 -0.534256 — 0.268274 -0.090600 -0.209789 — 0.198836 -0.166881 -0.362291

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000057 0.000095 0.000228 0.000266 0.000092 0.000087 0.000178 0.000055 0.000074 0.000090 0.000200 0.000111
Bud Neck 0.000202 0.000862 0.007153 0.003564 0.000470 0.000485 0.003760 0.001027 0.000329 0.000644 0.005234 0.001697
Bud Site 0.003436 0.002037 0.003688 0.011071 0.001903 0.002608 0.002792 0.003903 0.002711 0.002367 0.003181 0.005797
Cell Periphery 0.001952 0.001791 0.002307 0.000981 0.001698 0.002255 0.004777 0.000069 0.001832 0.002060 0.003704 0.000310
Cytoplasm 0.899673* 0.851490* 0.503765* 0.740882* 0.910839* 0.911082* 0.605408* 0.898815* 0.904956* 0.885986* 0.561246* 0.857090*
Cytoplasmic Foci 0.007427 0.008001 0.017894 0.009280 0.003925 0.008272 0.017383 0.002973 0.005770 0.008158 0.017605 0.004639
Eisosomes 0.000001 0.000001 0.000004 0.000004 0.000001 0.000001 0.000003 0.000001 0.000001 0.000001 0.000003 0.000002
Endoplasmic Reticulum 0.000168 0.000075 0.000121 0.000224 0.000106 0.000068 0.000088 0.000034 0.000139 0.000071 0.000102 0.000084
Endosome 0.000267 0.000190 0.000512 0.003404 0.000119 0.000196 0.000348 0.000270 0.000197 0.000193 0.000420 0.001098
Golgi 0.016439 0.007562 0.003249 0.005614 0.005446 0.005766 0.007501 0.000477 0.011238 0.006522 0.005654 0.001834
Lipid Particles 0.000890 0.001819 0.003837 0.002764 0.001393 0.001506 0.005267 0.000197 0.001128 0.001638 0.004646 0.000875
Mitochondria 0.000428 0.000194 0.000785 0.001681 0.000180 0.000136 0.000469 0.000566 0.000311 0.000161 0.000606 0.000860
Mitotic Spindle 0.001095 0.003672 0.008762 0.029527 0.001168 0.002103 0.006264 0.002808 0.001130 0.002764 0.007349 0.009867
None 0.004528 0.006676 0.009392 0.004935 0.006662 0.006160 0.007369 0.003354 0.005538 0.006377 0.008248 0.003772
Nuclear Periphery 0.000092 0.000387 0.002395 0.000670 0.000159 0.000146 0.001201 0.000108 0.000124 0.000247 0.001720 0.000256
Nuclear Periphery Foci 0.002196 0.004445 0.006080 0.006415 0.003278 0.002330 0.006610 0.000196 0.002708 0.003221 0.006380 0.001839
Nucleolus 0.000085 0.000121 0.000258 0.000443 0.000087 0.000081 0.000195 0.000278 0.000086 0.000098 0.000222 0.000322
Nucleus 0.015159 0.092027 0.405261* 0.143222* 0.048153 0.043342 0.281122* 0.055568 0.030769 0.063845 0.335058* 0.078725
Peroxisomes 0.000016 0.000010 0.000023 0.000376 0.000018 0.000013 0.000017 0.000120 0.000017 0.000012 0.000019 0.000188
Vacuole 0.010589 0.012908 0.022659 0.029853 0.008562 0.010559 0.045490 0.028072 0.009630 0.011548 0.035571 0.028542
Vacuole Periphery 0.035298 0.005636 0.001626 0.004822 0.005740 0.002805 0.003756 0.001110 0.021313 0.003997 0.002831 0.002091

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -2.21 -9.28 -5.38 -4.51 3.12 0.00 -4.67 -0.25 -0.27 2.35 -1.14 -9.67 -1.37 -0.97 3.28
Bud Neck -4.07 -8.19 -9.91 -7.26 4.80 -0.33 -9.55 -4.43 -5.92 8.72 -3.80 -12.15 -11.61 -7.94 9.68
Bud Site 2.45 -1.49 -3.80 -4.71 -1.86 -1.14 -2.99 -2.11 -0.73 0.95 0.48 -2.72 -3.44 -3.54 -0.18
Cell Periphery 0.05 -2.79 3.87 4.15 6.18 -1.23 -3.35 5.67 6.05 6.13 -1.03 -4.17 8.13 7.93 8.41
Cytoplasm 8.29 37.00 26.46 18.27 -16.71 0.99 31.15 8.16 6.28 -29.00 6.27 46.49 20.67 12.83 -36.89
Cytoplasmic Foci -1.66 -8.59 -4.64 -2.92 6.93 -6.55 -9.86 2.15 8.01 10.29 -5.46 -12.81 0.68 6.10 13.00
Eisosomes -0.23 -8.30 -4.83 -6.96 4.92 0.53 -4.90 0.29 -0.71 8.61 0.32 -9.24 -1.54 -4.17 10.67
Endoplasmic Reticulum 2.99 -0.59 0.50 -1.72 1.01 0.95 -1.07 1.86 1.98 8.22 2.61 -1.09 3.02 0.61 7.27
Endosome 1.24 -4.84 -3.26 -3.39 -2.18 -1.67 -6.53 -3.46 -0.98 3.87 -0.25 -7.60 -3.68 -3.54 -0.25
Golgi 5.83 10.37 10.08 4.00 0.84 -0.56 -0.37 8.77 9.50 6.96 5.38 7.71 14.40 9.97 7.45
Lipid Particles -3.06 -7.02 -7.18 -2.67 2.97 -0.72 -6.53 4.25 5.92 8.55 -2.94 -9.64 -0.41 2.87 9.68
Mitochondria 3.10 -6.04 -1.07 -1.58 0.41 1.03 -7.80 -1.76 -2.05 1.50 3.43 -8.92 -1.60 -2.50 1.72
Mitotic Spindle -2.52 -4.19 -5.50 -4.18 -1.67 -1.35 -3.35 -2.36 -0.88 2.30 -2.85 -5.35 -6.06 -3.47 1.28
None -3.39 -5.33 -0.73 3.68 6.00 0.86 -0.28 7.27 5.83 7.76 -1.85 -4.18 5.05 7.49 10.37
Nuclear Periphery -3.22 -7.52 -7.13 -2.58 5.93 0.15 -10.81 0.09 -0.03 10.79 -3.16 -11.33 -5.51 -1.02 10.34
Nuclear Periphery Foci -3.37 -4.28 -2.72 -0.03 1.61 1.77 -3.33 6.73 8.76 7.26 -1.56 -5.56 3.80 4.70 7.31
Nucleolus -1.56 -10.35 -3.38 -2.87 -0.30 0.18 -5.87 -6.98 -8.98 -1.77 -0.77 -11.04 -8.05 -7.79 -1.03
Nucleus -20.08 -34.55 -29.35 -14.51 16.95 1.07 -24.52 -8.57 -9.65 22.73 -15.61 -40.36 -29.48 -12.68 30.93
Peroxisomes 1.74 -6.68 -1.84 -1.89 -1.51 0.62 -1.71 -5.06 -6.12 -4.58 1.09 -4.49 -4.03 -4.25 -3.23
Vacuole -2.07 -6.98 -13.57 -11.65 -3.55 -2.21 -8.98 -16.89 -11.63 4.72 -2.89 -11.17 -21.44 -16.29 2.97
Vacuole Periphery 10.78 13.33 12.73 2.48 -1.41 2.74 2.56 5.08 3.94 2.95 11.38 12.96 13.97 4.63 2.23
Standard name
Human Ortholog
Description Bifunctional carbamoylphosphate synthetase/aspartate transcarbamylase; catalyzes the first two enzymatic steps in the de novo biosynthesis of pyrimidines; both activities are subject to feedback inhibition by UTP
Localization
Cell Percentages cytoplasm (100%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-2

Ura2

Ura2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ura2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available