Standard name
Human Ortholog
Description Ub-ligase substrate-specificity factor; proteolytically-cleaved form acts as a pseudosubstrate, binding and altering the substrate specificity of Ubr1p towards misfolded and native ER membrane and cytosolic proteins, as part of the stress-induced homeostatically regulated protein degradation (SHRED) pathway; hydrophilin essential during desiccation-rehydration; induced by osmotic stress, starvation and during stationary phase; protein abundance increases in response to DNA replication stress
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 – 0 0 0.05 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.99 0.98 0.97 0.97 0.92 0.86 – 0.62 0.65 0.62 0.58 0.54 0.98 0.98 0.99 0.86 0.77 0.87 0.95 0.92 0.89 0.95 0.93 0.89
Endoplasmic Reticulum 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0.08 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0.06 0.26 0.11 0.27 – 0.31 0.45 0.47 0.55 0.59 0 0 0 0 0.06 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0.07 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 – 0.15 0.09 0.09 0.07 0 0 0 0 0.07 0.16 0.09 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 2 0 – 1 0 6 3 2 0 0 0 3 1 1 2 1 1 0 0 0
Bud 3 0 4 5 2 1 – 6 5 8 13 11 0 0 1 1 1 0 1 1 0 0 1 4
Bud Neck 1 0 1 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 3 1 1 0 0 0
Bud Site 0 0 0 0 0 0 – 0 0 1 1 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 10 4 1 1 6 8 – 19 11 26 25 22 0 1 0 5 1 3 0 0 1 0 0 0
Cytoplasm 610 382 201 311 489 387 – 398 236 351 256 371 84 175 159 116 122 129 598 373 176 73 131 128
Endoplasmic Reticulum 3 1 2 0 12 4 – 4 6 12 3 17 0 0 0 3 8 6 0 1 2 0 0 0
Endosome 0 2 0 0 0 1 – 3 0 2 0 0 0 1 0 4 12 4 1 2 0 0 0 2
Golgi 1 0 0 0 0 0 – 0 0 0 0 0 0 0 0 2 2 1 3 4 0 0 0 0
Mitochondria 8 2 12 85 56 121 – 198 163 267 245 402 3 2 1 5 9 1 6 8 8 3 3 0
Nucleus 0 1 2 0 2 1 – 9 1 1 2 3 1 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 1 0 0 0 0 0 – 12 3 3 4 10 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 – 2 1 0 0 0 0 0 0 3 11 3 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 1 0 0 0 0 0 – 0 0 1 0 0 0 0 0 2 6 1 1 0 0 0 0 2
Vac/Vac Membrane 0 2 0 0 8 9 – 96 32 52 29 27 0 0 1 10 25 14 3 4 0 0 1 1
Unique Cell Count 619 389 207 322 530 452 638 363 570 442 683 86 178 160 135 159 148 629 404 199 77 141 144
Labelled Cell Count 638 394 223 402 577 532 748 458 730 581 865 88 179 162 154 198 163 629 404 199 77 141 144


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.3 5.0 3.5 4.1 7.1 6.9 – 6.6 6.0 6.2 5.6 5.4 5.0 5.7 5.7 8.6 9.3 8.4 4.6 5.2 5.7
Std Deviation (1e-4) 0.6 1.0 1.0 1.1 1.5 1.5 – 1.7 1.8 2.1 2.4 1.9 1.0 1.1 1.1 4.3 4.2 3.8 0.8 1.3 1.5
Intensity Change (Log2) – – – 0.24 1.03 1.0 – 0.94 0.78 0.84 0.68 0.63 0.52 0.73 0.72 1.32 1.42 1.28 0.39 0.57 0.72

WT3RAP60RAP140RAP220RAP380RAP460RAP540RAP620RAP7000510WT3HU80HU120HU1600510WT3rpd3Δ_1rpd3Δ_2rpd3Δ_30510WT1AF100AF140AF1800510
rapamycinhydroxy-urearpd3 knockoutalpha-factorMultiple Condition Time-Course Intensity PlotsGFP Intensity (1e-4)GFP Intensity (1e-4)GFP Intensity (1e-4)GFP Intensity (1e-4)

Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 – 0 0 0 0.7 0 0 0 0 0 0 0
Bud Neck 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 – 0 0 2.7 3.1 2.2 0 0 0 0 0 0
Cytoplasm -0.3 -2.4 -4.4 – -9.6 -8.7 -9.7 -10.2 -11.3 0.3 0.8 1.6 -3.9 -6.0 -3.6
Endoplasmic Reticulum 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 – 0 0 0 0 0 0 0 0 0 4.0 0
Golgi 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 2.0 6.2 – 7.3 9.7 10.5 12.0 13.4 0 -2.4 -2.7 -0.9 -0.1 -2.5
Nucleus 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 – 5.9 4.4 4.5 3.8 2.9 0 0 0 0 5.9 4.5

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 0.5969 1.5754 0.961 0.9132 0.7666 1.0945 -0.3929 0.3872 0.1532 0.6008 -0.282 0.3192 0.9829 2.396 1.888 1.8201 1.954 1.9276
Actin 0.0006 0.0006 0.0003 0.0001 0.0003 0.0013 0.0176 0.0045 0.011 0.0027 0.0021 0.0107 0.0119 0.0004 0.0004 0.0001 0.0001 0.0013
Bud 0.0036 0.001 0.0004 0.0005 0.0002 0.0002 0.0029 0.0027 0.0032 0.0007 0.0006 0.001 0.001 0.0071 0.0014 0.0002 0.0001 0.0001
Bud Neck 0.0003 0.0004 0.0002 0.0001 0.0002 0.0012 0.0143 0.0002 0.0008 0.0205 0.0001 0.0061 0.003 0.0001 0.0001 0.0001 0.0002 0.0026
Bud Periphery 0.0021 0.0006 0.0004 0.0008 0.0002 0.0001 0.007 0.0067 0.0037 0.0007 0.0008 0.0024 0.0014 0.0016 0.0004 0.0001 0.0001 0.0001
Bud Site 0.0027 0.0084 0.0006 0.0003 0.0001 0.0002 0.0091 0.012 0.0093 0.0073 0.0007 0.0004 0.0067 0.0026 0.0013 0.0001 0.0001 0.0001
Cell Periphery 0.0003 0.0002 0.0001 0.0001 0 0 0.0007 0.0004 0.0003 0.0003 0 0.0001 0.0004 0.0004 0.0001 0 0.0001 0
Cytoplasm 0.3291 0.5786 0.5034 0.4264 0.4887 0.56 0.3022 0.5541 0.4977 0.457 0.5769 0.569 0.3615 0.6009 0.5658 0.6965 0.6471 0.6823
Cytoplasmic Foci 0.0181 0.0187 0.0136 0.0164 0.0117 0.0201 0.042 0.0132 0.018 0.0244 0.0165 0.0252 0.0223 0.0116 0.0094 0.011 0.0103 0.0154
Eisosomes 0.0001 0.0001 0.0001 0 0 0 0.0004 0.0001 0.0001 0.0001 0 0.0001 0.0003 0.0001 0 0 0 0
Endoplasmic Reticulum 0.0041 0.0023 0.0036 0.0024 0.0039 0.0024 0.0051 0.0026 0.0038 0.0067 0.0007 0.0034 0.0045 0.0017 0.0024 0.0015 0.0027 0.0014
Endosome 0.0117 0.0069 0.0129 0.0077 0.0097 0.0098 0.0305 0.0052 0.0141 0.0232 0.0073 0.0161 0.0126 0.0021 0.0047 0.0076 0.0059 0.0077
Golgi 0.0007 0.0004 0.0004 0.0002 0.0003 0.0006 0.0077 0.0005 0.0044 0.0085 0.0023 0.0051 0.0021 0.0003 0.0002 0.0003 0.0002 0.0004
Lipid Particles 0.0008 0.0004 0.0005 0.0002 0.0001 0.0003 0.0089 0.0003 0.0039 0.0137 0.0034 0.0021 0.0029 0.0004 0.0001 0 0.0001 0.0002
Mitochondria 0.0017 0.0005 0.0007 0.0005 0.0004 0.0004 0.0049 0.0004 0.0106 0.0034 0.0015 0.0029 0.002 0.0011 0.0004 0.0003 0.0002 0.0005
None 0.609 0.3724 0.453 0.5357 0.4772 0.3961 0.5145 0.3917 0.4028 0.4075 0.3538 0.3452 0.5461 0.3651 0.4084 0.2781 0.3291 0.2817
Nuclear Periphery 0.0026 0.0008 0.0016 0.0009 0.0016 0.0007 0.0041 0.0009 0.0022 0.0019 0.0007 0.0016 0.0024 0.0004 0.0007 0.0004 0.0006 0.0015
Nucleolus 0.0006 0.0004 0.0002 0.0002 0.0001 0.0001 0.0024 0.0002 0.0003 0.001 0.0001 0.0001 0.001 0.0005 0.0002 0 0 0.0002
Nucleus 0.0053 0.0025 0.003 0.0016 0.0021 0.0017 0.0034 0.0011 0.0023 0.0013 0.0009 0.0012 0.0047 0.0013 0.0012 0.0008 0.0009 0.002
Peroxisomes 0.0004 0.0002 0.0002 0.0001 0.0001 0.002 0.0131 0.0002 0.0066 0.0129 0.0267 0.0036 0.0037 0.0003 0.0001 0.0001 0.0001 0.0002
Punctate Nuclear 0.0018 0.0022 0.0009 0.0008 0.0007 0.0012 0.0021 0.0008 0.0019 0.001 0.0038 0.0011 0.0068 0.0007 0.0005 0.0003 0.0003 0.0015
Vacuole 0.0036 0.0022 0.0037 0.0045 0.0021 0.0014 0.006 0.0019 0.0024 0.004 0.0008 0.0019 0.0024 0.0012 0.0019 0.0019 0.0016 0.0008
Vacuole Periphery 0.0006 0.0002 0.0006 0.0006 0.0004 0.0002 0.0011 0.0003 0.0007 0.0014 0.0002 0.0009 0.0004 0.0002 0.0003 0.0004 0.0002 0.0002

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression – – – – – – – – – –
Translational Efficiency – – – – – – – – – –

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression –
Translational Efficiency –

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
194 1590 1550 612 255 1758 1286 448 449 3348 2836 1060

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 675.00 759.17 884.39 896.77 691.96 783.30 853.05 985.35 684.63 771.84 870.18 934.21
Standard Deviation 72.76 100.17 108.46 174.60 84.11 97.33 144.31 231.91 79.85 99.42 126.95 205.54
Intensity Change Log 2 — 0.169535 0.389795 0.409851 — 0.178876 0.301942 0.509948 — 0.174271 0.345992 0.461388

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000067 0.000218 0.000416 0.000403 0.000276 0.000223 0.000505 0.000918 0.000186 0.000221 0.000457 0.000621
Bud Neck 0.013809 0.022997 0.003624 0.003822 0.019534 0.045492 0.005445 0.003853 0.017061 0.034809 0.004450 0.003835
Bud Site 0.002738 0.012789 0.005727 0.012897 0.005374 0.018180 0.019624 0.026037 0.004235 0.015620 0.012029 0.018451
Cell Periphery 0.000225 0.000127 0.000127 0.000163 0.000252 0.000109 0.000120 0.000155 0.000240 0.000118 0.000123 0.000159
Cytoplasm 0.429094 0.529600 0.446755 0.550311 0.413062 0.405600 0.627376 0.536920 0.419989 0.464489 0.528658 0.544651
Cytoplasmic Foci 0.280484 0.209435 0.010991 0.043574 0.313903 0.249182 0.029806 0.084676 0.299463 0.230306 0.019523 0.060945
Eisosomes 0.000201 0.000095 0.000029 0.000052 0.000377 0.000136 0.000033 0.000042 0.000301 0.000117 0.000031 0.000048
Endoplasmic Reticulum 0.001031 0.000827 0.002908 0.002139 0.001048 0.000754 0.002425 0.001184 0.001041 0.000788 0.002689 0.001735
Endosome 0.018544 0.015920 0.002282 0.007286 0.020896 0.044700 0.002971 0.019707 0.019880 0.031032 0.002595 0.012536
Golgi 0.001441 0.005406 0.000097 0.002193 0.003384 0.009609 0.000381 0.004223 0.002545 0.007613 0.000226 0.003051
Lipid Particles 0.013457 0.003801 0.001140 0.001993 0.010344 0.005350 0.000962 0.002954 0.011689 0.004614 0.001059 0.002399
Mitochondria 0.011229 0.007710 0.000617 0.010563 0.005813 0.015669 0.000905 0.009071 0.008153 0.011889 0.000748 0.009932
Mitotic Spindle 0.000348 0.005528 0.010858 0.014080 0.001094 0.006713 0.024694 0.060117 0.000772 0.006150 0.017132 0.033537
None 0.002966 0.002848 0.004563 0.003469 0.001109 0.000925 0.003364 0.004555 0.001911 0.001838 0.004020 0.003928
Nuclear Periphery 0.000643 0.000354 0.001592 0.000999 0.000495 0.000379 0.000914 0.000722 0.000559 0.000367 0.001284 0.000882
Nuclear Periphery Foci 0.000366 0.000350 0.001246 0.003000 0.000528 0.000257 0.000896 0.002913 0.000458 0.000301 0.001088 0.002963
Nucleolus 0.001789 0.000883 0.000383 0.000450 0.000678 0.000765 0.000220 0.000692 0.001158 0.000821 0.000309 0.000553
Nucleus 0.161628 0.109970 0.467436 0.135091 0.125139 0.059768 0.187163 0.054829 0.140905 0.083609 0.340344 0.101169
Peroxisomes 0.006014 0.006869 0.001268 0.000868 0.009275 0.009652 0.001102 0.009714 0.007866 0.008330 0.001193 0.004607
Vacuole 0.052960 0.062464 0.037807 0.204834 0.065531 0.122424 0.090832 0.173132 0.060100 0.093948 0.061851 0.191436
Vacuole Periphery 0.000965 0.001811 0.000135 0.001812 0.001888 0.004114 0.000261 0.003585 0.001489 0.003020 0.000192 0.002561

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -5.62 -2.70 -5.99 -3.08 0.47 0.71 -1.17 -1.17 -1.70 -0.07 -0.84 -2.59 -3.28 -3.07 0.33
Bud Neck -2.49 2.73 2.94 11.42 0.92 -6.05 3.64 4.30 17.40 2.78 -6.15 4.62 5.18 20.55 2.47
Bud Site -6.41 -1.83 -3.95 0.78 -2.81 -7.27 -3.97 -3.88 0.86 -0.28 -9.54 -4.02 -5.32 1.49 -1.84
Cell Periphery 2.89 2.44 2.19 -1.00 -0.12 4.25 4.05 4.63 1.60 2.08 5.09 4.57 4.57 -0.54 0.61
Cytoplasm -4.86 1.37 -1.68 4.44 -4.76 0.31 -7.09 -0.99 -1.64 6.29 -3.42 -3.27 -2.18 1.12 0.96
Cytoplasmic Foci 4.31 16.91 15.20 30.54 -10.35 4.71 22.18 18.94 28.24 -5.88 6.54 27.90 24.72 42.72 -10.38
Eisosomes 5.34 8.67 7.87 5.59 -4.28 3.15 4.43 4.38 13.70 -1.17 4.15 5.93 5.71 13.31 -4.25
Endoplasmic Reticulum 0.92 -11.21 -4.64 -6.68 6.25 0.87 -2.29 0.07 -1.13 2.49 1.18 -7.09 -3.45 -5.67 5.15
Endosome 0.64 3.91 3.24 8.40 -4.30 -5.58 4.90 2.89 12.94 -3.34 -3.68 6.27 4.55 16.20 -4.79
Golgi -5.00 2.93 0.64 5.34 -1.76 -4.99 3.22 2.01 9.28 -2.48 -6.50 4.07 2.10 10.39 -2.75
Lipid Particles 3.71 4.67 4.25 3.27 -3.29 2.24 4.20 3.68 4.02 -3.40 4.18 6.24 5.57 5.03 -4.67
Mitochondria 0.63 1.83 1.07 1.25 -1.89 -3.88 2.40 1.25 5.95 -0.98 -1.25 2.69 1.42 4.82 -2.13
Mitotic Spindle -4.93 -5.75 -5.11 -3.64 -2.13 -3.85 -8.85 -7.21 -6.50 -3.36 -5.85 -10.38 -8.70 -7.32 -3.79
None 0.11 -0.78 -0.02 -0.38 2.55 0.41 -4.83 -2.96 -3.57 -0.40 0.13 -2.54 -1.66 -3.30 1.29
Nuclear Periphery 2.67 -11.60 -5.44 -10.43 5.45 0.68 -7.27 -3.99 -6.11 3.39 1.80 -12.52 -6.51 -11.18 6.23
Nuclear Periphery Foci -0.03 -9.46 -8.59 -8.73 -5.24 1.89 -3.95 -5.64 -6.19 -4.61 1.69 -8.88 -9.82 -10.46 -6.99
Nucleolus 1.09 1.37 1.60 4.15 0.73 -0.56 2.49 0.75 1.73 -1.99 0.90 1.77 1.78 4.12 -0.14
Nucleus 3.90 -22.25 1.71 -3.56 35.64 6.10 -8.39 5.48 -0.38 20.42 6.81 -23.36 3.84 -4.32 37.22
Peroxisomes -0.55 3.20 3.66 10.18 0.59 -0.23 4.67 2.37 3.49 -1.66 -0.39 5.58 4.34 7.85 -1.24
Vacuole -2.03 -1.05 -19.36 -20.19 -20.65 -7.76 -9.21 -17.82 -14.88 -13.15 -7.02 -6.79 -26.04 -24.11 -23.57
Vacuole Periphery -3.47 5.64 0.19 1.78 -1.50 -2.78 2.28 0.97 5.17 -1.61 -3.29 3.12 1.01 5.18 -2.16
Protein Complexes26S Proteasome complex37S mitochondrial small ribosomal subunit54S mitochondrial large ribosomal subunit60S cytosolic large ribosomal subunitAar2-U5 snRNPAnaphase-Promoting Complex variant 1Anaphase-Promoting Complex variant 2Anaphase-Promoting Complex variant 3Anaphase-Promoting Complex variant 4Arp2/3 complexATG1 kinase complexBLOC-1 complexCcr4-Not complexCentral kinetochore CTF19 complexCleavage and polyadenylation specificity factorcomplexCMG helicase complexCMG-Pol epsilon complexCOMPASS complexCOP9 signalosome COPI vesicle coat complexCoQ6 biosynthesis complexCore mediator complexCORVET complexCTF18-RFC complexCURI complex variant 1CURI complex variant 2CURI complex variant 3Cytoplasmic exosome complex, DIS3 variantDASH complexDNA-directed RNA Polymerase I complexDNA-directed RNA polymerase II complexDNA-directed RNA polymerase III complexDNA replication factor C complexEGO complexELG1-RFC complex Elongator holoenzyme complexEndoplasmic Reticulum Membrane ComplexEukaryotic translation initiation factor 2BcomplexEukaryotic translation initiation factor 3core complexExocystExomer complexFAR complexGeneral transcription factor complex TFIIDGeneral transcription factor complex TFIIHGET4-GET5 transmembrane domain recognition complexGID ubiquitin ligase complexGin4 complexGolgi transport complexGSE complexHMC complexHOPS complexINO80 chromatin remodeling complexLSM1-7 complexLsm1-7-Pat1 complexLSM2-7 complexLSM2-8 complexMCM complexMICOS complexMitochondrial electron transport complex IIIMitochondrial inner membrane pre-sequence translocasecomplexMitochondrial outer membrane translocase holocomplexMitochondrial proton-transporting ATP synthase complexMitochondrial pyruvate dehydrogenase complexNineTeen complexNOP8 60s ribosome pre-assembly complexNuA3 histone acetyltransferase complexNuA4 histone acetyltransferase complexNuclear condensin complexNuclear/nucleolar exosome complex, DIS3-RRP6 variantNuclear pore complexNucleolar ribonuclease MRP complexNucleolar ribonuclease P complexOligosaccharyl transferase complex variant 1Oligosaccharyl transferase complex variant 2PAF1 complexPAS complexPhosphatidylinositol 3-kinase complex IPolarisomePrefoldin complexPRP19-associated complexRad17 RFC-like complexRetromer complexRPD3L histone deacetylase complexRPD3S histone deacetylase complexRQC complexRSC complex variant RSC1RSC complex variant RSC2SAGA complexSCF-Met30 ubiquitin ligase complexSCF-Ufo1 ubiquitin ligase complexSEA complexSeptin complexSET3C histone deacetylase complexSignal recognition particleSLIK (SAGA-like) complexSmall ribosomal subunit processome, variant1Small ribosomal subunit processome, variant2Small ribosomal subunit processome, variant3SMC5-SMC6 SUMO ligase complexSm complexSpliceosomal commitment complexSPOTS complexSWI/SNF chromatin remodelling complexSwr1 chromatin remodelling complexTHO complexTranscription factor TFIIIC complexTranslocon complexTRAPPIII protein complexTRAPPII protein complexTRAPPI protein complexTREX complexU1 snRNPU2 snRNPU4 snRNPU4/U6 snRNPU4/U6.U5 tri-snRNP complexU5 snRNPU6 snRNP complexUTP-A complexUTP-B complexUTP-C complex variant 1UTP-C complex variant 2UTP-C complex variant 3Vacuolar proton translocating ATPase complex,Golgi variantVacuolar proton translocating ATPase complex,vacuole variantCellular Components••••••••••••••••actinbud neckcell peripherycytoplasmendosomeERGolgimitochondrionmixednuclear peripherynucleolusnucleusperoxisomespindle polevacuolar membranevacuole
Standard name
Human Ortholog
Description Ub-ligase substrate-specificity factor; proteolytically-cleaved form acts as a pseudosubstrate, binding and altering the substrate specificity of Ubr1p towards misfolded and native ER membrane and cytosolic proteins, as part of the stress-induced homeostatically regulated protein degradation (SHRED) pathway; hydrophilin essential during desiccation-rehydration; induced by osmotic stress, starvation and during stationary phase; protein abundance increases in response to DNA replication stress
Localization
Cell Percentages cytoplasm (27%), nucleus (5%), mixed (41%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Roq1

Roq1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Roq1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available