Standard name | |
---|---|
Human Ortholog | |
Description | CDK inhibitor and nuclear anchor; during the cell cycle Far1p sequesters the GEF Cdc24p in the nucleus; phosphorylation by Cdc28p-Cln results in SCFCdc4 complex-mediated ubiquitin-dependent degradation, releasing Cdc24p for export and activation of GTPase Cdc42p; in response to pheromone, phosphorylation of Far1p by MAPK Fus3p results in association with, and inhibition of Cdc28p-Cln, as well as Msn5p mediated nuclear export of Far1p-Cdc24p, targeting Cdc24p to polarity sites |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 3 | 3 | 2 | 9 | 11 | 12 |
Bud | 0 | 1 | 0 | 0 | 0 | 2 | 3 | 1 | 6 | 5 | 7 | 2 | 3 | 0 | 1 | 0 | 0 | 0 | 24 | 15 | 1 | 20 | 11 | 17 |
Bud Neck | 0 | 3 | 3 | 0 | 2 | 1 | 3 | 6 | 5 | 3 | 0 | 7 | 0 | 0 | 0 | 1 | 0 | 0 | 5 | 5 | 0 | 8 | 3 | 12 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 2 | 3 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 1 | 2 | 2 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 2 | 1 | 1 | 2 |
Cytoplasm | 227 | 248 | 126 | 53 | 145 | 81 | 190 | 130 | 122 | 131 | 147 | 124 | 369 | 431 | 549 | 53 | 34 | 13 | 163 | 190 | 85 | 181 | 245 | 229 |
Endoplasmic Reticulum | 2 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 5 | 3 | 3 | 0 | 0 | 2 | 5 | 6 | 4 |
Endosome | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 2 | 2 | 2 | 4 | 0 | 1 | 7 | 2 | 0 | 4 | 1 | 3 |
Golgi | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 1 | 0 | 2 | 5 | 1 |
Mitochondria | 19 | 6 | 0 | 9 | 2 | 17 | 25 | 21 | 51 | 70 | 72 | 57 | 17 | 7 | 4 | 2 | 4 | 0 | 12 | 9 | 6 | 22 | 22 | 13 |
Nucleus | 59 | 34 | 9 | 7 | 4 | 2 | 5 | 5 | 8 | 13 | 5 | 6 | 31 | 15 | 0 | 1 | 0 | 1 | 69 | 37 | 13 | 11 | 6 | 17 |
Nuclear Periphery | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 1 | 2 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
SpindlePole | 1 | 0 | 1 | 0 | 0 | 0 | 2 | 1 | 0 | 3 | 1 | 1 | 0 | 0 | 1 | 1 | 2 | 0 | 28 | 18 | 4 | 5 | 7 | 11 |
Vac/Vac Membrane | 41 | 29 | 9 | 0 | 2 | 1 | 2 | 2 | 3 | 3 | 2 | 5 | 38 | 39 | 11 | 6 | 2 | 1 | 27 | 22 | 13 | 42 | 40 | 62 |
Unique Cell Count | 323 | 304 | 141 | 63 | 152 | 94 | 209 | 148 | 162 | 189 | 193 | 169 | 440 | 472 | 561 | 64 | 39 | 16 | 353 | 319 | 142 | 325 | 372 | 400 |
Labelled Cell Count | 352 | 326 | 150 | 70 | 155 | 104 | 231 | 168 | 197 | 231 | 240 | 206 | 462 | 496 | 572 | 76 | 45 | 20 | 353 | 319 | 142 | 325 | 372 | 400 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 4.4 | 4.2 | 5.1 | 4.1 | 4.8 | 3.6 | 4.0 | 4.0 | 3.4 | 3.2 | 2.9 | 3.4 | 4.2 | 4.2 | 4.2 | 5.9 | 5.5 | 5.5 | 4.9 | 4.9 | 5.0 |
Std Deviation (1e-4) | 0.9 | 1.0 | 1.6 | 1.2 | 1.6 | 1.7 | 1.5 | 1.3 | 1.2 | 1.2 | 0.8 | 1.3 | 1.1 | 1.5 | 1.3 | 1.7 | 1.7 | 1.5 | 1.7 | 1.3 | 1.3 |
Intensity Change (Log2) | – | – | – | -0.32 | -0.11 | -0.53 | -0.37 | -0.35 | -0.6 | -0.69 | -0.81 | -0.58 | -0.3 | -0.29 | -0.29 | 0.21 | 0.1 | 0.1 | -0.07 | -0.07 | -0.02 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 3.6151 | 2.5469 | 1.8802 | 1.2839 | 1.7906 | 2.6886 | 1.7273 | 0.7061 | 0.1326 | -0.0519 | -0.5404 | 0.7519 | 1.3347 | 0.1146 | -0.3964 | -0.22 | -0.6986 | 0.4621 |
Actin | 0.0003 | 0.0005 | 0.0005 | 0.0002 | 0.0004 | 0.0006 | 0.0004 | 0.0007 | 0.0013 | 0.0004 | 0.0009 | 0.0011 | 0.0002 | 0.0007 | 0.0008 | 0.0006 | 0.0005 | 0.0009 |
Bud | 0.0007 | 0.002 | 0.0031 | 0.0026 | 0.0064 | 0.0009 | 0.0023 | 0.0136 | 0.0081 | 0.0006 | 0.0099 | 0.0011 | 0.0011 | 0.005 | 0.005 | 0.0038 | 0.0017 | 0.0012 |
Bud Neck | 0.0008 | 0.001 | 0.0013 | 0.0012 | 0.0024 | 0.0059 | 0.0013 | 0.0014 | 0.0026 | 0.0005 | 0.0033 | 0.0066 | 0.0009 | 0.0012 | 0.0016 | 0.0009 | 0.0012 | 0.0046 |
Bud Periphery | 0.001 | 0.0014 | 0.0027 | 0.0028 | 0.011 | 0.0012 | 0.0022 | 0.0043 | 0.008 | 0.0006 | 0.0118 | 0.001 | 0.0008 | 0.0027 | 0.0045 | 0.0037 | 0.0017 | 0.0011 |
Bud Site | 0.0013 | 0.0062 | 0.0027 | 0.0016 | 0.002 | 0.0008 | 0.0074 | 0.0177 | 0.0123 | 0.0002 | 0.0029 | 0.0006 | 0.0011 | 0.0082 | 0.0048 | 0.0011 | 0.0006 | 0.0003 |
Cell Periphery | 0.0002 | 0.0002 | 0.0002 | 0.0001 | 0.0004 | 0.0001 | 0.0003 | 0.0004 | 0.0004 | 0 | 0.0003 | 0.0001 | 0.0001 | 0.0002 | 0.0002 | 0.0001 | 0.0001 | 0.0001 |
Cytoplasm | 0.263 | 0.2262 | 0.1358 | 0.0906 | 0.1274 | 0.2162 | 0.2729 | 0.3012 | 0.2645 | 0.2805 | 0.2291 | 0.2613 | 0.2363 | 0.1944 | 0.1608 | 0.1824 | 0.2022 | 0.212 |
Cytoplasmic Foci | 0.0038 | 0.0042 | 0.0056 | 0.0038 | 0.0066 | 0.0068 | 0.0051 | 0.0047 | 0.0101 | 0.0047 | 0.0168 | 0.0071 | 0.0034 | 0.0056 | 0.0052 | 0.0049 | 0.0063 | 0.0045 |
Eisosomes | 0 | 0.0001 | 0.0001 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0 | 0.0001 | 0.0001 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0.0039 | 0.0034 | 0.0023 | 0.0006 | 0.003 | 0.002 | 0.0028 | 0.0043 | 0.0036 | 0.0024 | 0.0036 | 0.0027 | 0.0026 | 0.003 | 0.0022 | 0.0029 | 0.003 | 0.0018 |
Endosome | 0.0018 | 0.0018 | 0.0017 | 0.0006 | 0.0034 | 0.0021 | 0.0025 | 0.0034 | 0.0034 | 0.0024 | 0.0045 | 0.0028 | 0.0017 | 0.0025 | 0.0018 | 0.0026 | 0.0035 | 0.0021 |
Golgi | 0.0001 | 0.0001 | 0.0001 | 0 | 0.0001 | 0.0003 | 0.0001 | 0.0002 | 0.0003 | 0.0001 | 0.0003 | 0.0001 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0.0001 |
Lipid Particles | 0.0002 | 0.0001 | 0.0003 | 0.0002 | 0.0003 | 0.0002 | 0.0003 | 0.0001 | 0.0003 | 0.0001 | 0.0005 | 0.0001 | 0.0002 | 0.0002 | 0.0002 | 0.0002 | 0.0005 | 0.0001 |
Mitochondria | 0.0006 | 0.001 | 0.0012 | 0.0007 | 0.0015 | 0.0008 | 0.0005 | 0.0017 | 0.0019 | 0.0011 | 0.0019 | 0.0008 | 0.0005 | 0.001 | 0.0011 | 0.0014 | 0.0013 | 0.0006 |
None | 0.2781 | 0.4697 | 0.6892 | 0.8328 | 0.6906 | 0.3413 | 0.2066 | 0.3134 | 0.4842 | 0.5685 | 0.4919 | 0.2198 | 0.2879 | 0.4581 | 0.6321 | 0.5752 | 0.5893 | 0.3461 |
Nuclear Periphery | 0.0075 | 0.0143 | 0.0041 | 0.0014 | 0.0054 | 0.0053 | 0.0083 | 0.009 | 0.0081 | 0.0074 | 0.0099 | 0.0081 | 0.0087 | 0.0079 | 0.0069 | 0.0102 | 0.0137 | 0.0065 |
Nucleolus | 0.0025 | 0.0035 | 0.0041 | 0.0023 | 0.005 | 0.0041 | 0.0023 | 0.0059 | 0.0041 | 0.0011 | 0.0049 | 0.0037 | 0.0016 | 0.0035 | 0.0036 | 0.0034 | 0.0058 | 0.0034 |
Nucleus | 0.4226 | 0.2545 | 0.1331 | 0.0519 | 0.1116 | 0.386 | 0.4681 | 0.2971 | 0.163 | 0.1207 | 0.1747 | 0.4473 | 0.4371 | 0.2807 | 0.1495 | 0.1852 | 0.1453 | 0.3852 |
Peroxisomes | 0.0001 | 0.0002 | 0.0003 | 0.0002 | 0.0005 | 0.0004 | 0.0001 | 0.0003 | 0.0006 | 0.0003 | 0.0012 | 0.0003 | 0.0001 | 0.0006 | 0.0003 | 0.0004 | 0.0004 | 0.0002 |
Punctate Nuclear | 0.0078 | 0.0062 | 0.0087 | 0.0049 | 0.0093 | 0.023 | 0.0123 | 0.0156 | 0.0168 | 0.0057 | 0.021 | 0.0327 | 0.0124 | 0.02 | 0.0159 | 0.0166 | 0.0184 | 0.0275 |
Vacuole | 0.0034 | 0.0028 | 0.0027 | 0.0011 | 0.011 | 0.0017 | 0.0035 | 0.0044 | 0.0054 | 0.0021 | 0.0088 | 0.0024 | 0.0027 | 0.0037 | 0.0029 | 0.0035 | 0.0036 | 0.0015 |
Vacuole Periphery | 0.0004 | 0.0005 | 0.0004 | 0.0002 | 0.0015 | 0.0003 | 0.0004 | 0.0006 | 0.0008 | 0.0005 | 0.0016 | 0.0004 | 0.0004 | 0.0005 | 0.0005 | 0.0007 | 0.0009 | 0.0003 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 5.0899 | 5.5392 | 42.5714 | 85.4723 | 22.6671 | 7.9624 | 7.801 | 16.0654 | 47.7086 | 23.5309 |
Translational Efficiency | 0.4033 | 0.5872 | 0.7103 | 0.6567 | 0.6339 | 0.4172 | 0.5944 | 0.6214 | 0.738 | 0.7544 |
Dataset | Hit |
---|---|
Protein Concentration | ✔ |
Protein Localization | ✘ |
Gene Expression | ✔ |
Translational Efficiency | ✔ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
1979 | 1676 | 966 | 1501 | 1380 | 1936 | 369 | 2478 | 3359 | 3612 | 1335 | 3979 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 637.43 | 759.11 | 895.47 | 781.12 | 641.23 | 722.54 | 793.34 | 833.14 | 638.99 | 739.51 | 867.24 | 813.52 |
Standard Deviation | 66.86 | 112.20 | 118.16 | 98.24 | 87.19 | 101.77 | 102.15 | 104.86 | 75.90 | 108.28 | 122.77 | 105.47 |
Intensity Change Log 2 | — | 0.252042 | 0.490378 | 0.293277 | — | 0.172236 | 0.307097 | 0.377717 | — | 0.212572 | 0.401374 | 0.336240 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000061 | 0.000326 | 0.000404 | 0.001300 | 0.000097 | 0.000570 | 0.000117 | 0.000618 | 0.000076 | 0.000457 | 0.000325 | 0.000876 |
Bud Neck | 0.019843 | 0.019657 | 0.009938 | 0.009084 | 0.008791 | 0.030864 | 0.001562 | 0.012575 | 0.015302 | 0.025664 | 0.007623 | 0.011258 |
Bud Site | 0.003581 | 0.010765 | 0.014302 | 0.029413 | 0.001419 | 0.009536 | 0.001856 | 0.026688 | 0.002693 | 0.010107 | 0.010862 | 0.027716 |
Cell Periphery | 0.000166 | 0.000234 | 0.000128 | 0.000176 | 0.000174 | 0.000189 | 0.000888 | 0.000147 | 0.000169 | 0.000210 | 0.000338 | 0.000158 |
Cytoplasm | 0.494452* | 0.377992* | 0.121656* | 0.383678* | 0.430385* | 0.398207* | 0.219681* | 0.444339* | 0.468131* | 0.388827* | 0.148751* | 0.421456* |
Cytoplasmic Foci | 0.162665 | 0.102915 | 0.003427 | 0.012908 | 0.141780 | 0.112341 | 0.022759 | 0.008738 | 0.154085 | 0.107967 | 0.008771 | 0.010311 |
Eisosomes | 0.000170 | 0.000087 | 0.000048 | 0.000041 | 0.000102 | 0.000075 | 0.000480 | 0.000033 | 0.000142 | 0.000081 | 0.000168 | 0.000036 |
Endoplasmic Reticulum | 0.000700 | 0.001994 | 0.006536 | 0.002684 | 0.000831 | 0.001290 | 0.001389 | 0.001521 | 0.000754 | 0.001617 | 0.005113 | 0.001960 |
Endosome | 0.007196 | 0.008619 | 0.001703 | 0.004066 | 0.005222 | 0.009294 | 0.001657 | 0.003010 | 0.006385 | 0.008981 | 0.001690 | 0.003408 |
Golgi | 0.001311 | 0.003015 | 0.000062 | 0.002554 | 0.001439 | 0.002083 | 0.000039 | 0.001270 | 0.001363 | 0.002516 | 0.000056 | 0.001754 |
Lipid Particles | 0.008392 | 0.003774 | 0.001575 | 0.001090 | 0.007704 | 0.004937 | 0.001939 | 0.000611 | 0.008110 | 0.004397 | 0.001676 | 0.000791 |
Mitochondria | 0.003444 | 0.007354 | 0.000930 | 0.004766 | 0.004033 | 0.008769 | 0.000333 | 0.004061 | 0.003686 | 0.008113 | 0.000765 | 0.004327 |
Mitotic Spindle | 0.001267 | 0.006261 | 0.003047 | 0.018786 | 0.000480 | 0.007414 | 0.000055 | 0.029003 | 0.000944 | 0.006879 | 0.002220 | 0.025149 |
None | 0.005863 | 0.007399 | 0.004328 | 0.004913 | 0.013164 | 0.008939 | 0.013194 | 0.007436 | 0.008862 | 0.008224 | 0.006779 | 0.006485 |
Nuclear Periphery | 0.000493 | 0.000908 | 0.002770 | 0.001679 | 0.000806 | 0.000794 | 0.000845 | 0.001835 | 0.000621 | 0.000847 | 0.002238 | 0.001776 |
Nuclear Periphery Foci | 0.000323 | 0.000548 | 0.000787 | 0.000921 | 0.000533 | 0.000403 | 0.000233 | 0.000364 | 0.000409 | 0.000470 | 0.000634 | 0.000574 |
Nucleolus | 0.001506 | 0.003115 | 0.001037 | 0.000367 | 0.002542 | 0.003305 | 0.001026 | 0.000514 | 0.001932 | 0.003217 | 0.001034 | 0.000459 |
Nucleus | 0.251350* | 0.396787* | 0.785790* | 0.448320* | 0.353493* | 0.356978* | 0.706587* | 0.396299* | 0.293314* | 0.375450* | 0.763898* | 0.415923* |
Peroxisomes | 0.003320 | 0.008650 | 0.000192 | 0.000808 | 0.004314 | 0.008489 | 0.000220 | 0.002238 | 0.003729 | 0.008564 | 0.000200 | 0.001699 |
Vacuole | 0.033375 | 0.037038 | 0.041121 | 0.068825 | 0.021892 | 0.034475 | 0.025017 | 0.057739 | 0.028657 | 0.035664 | 0.036670 | 0.061921 |
Vacuole Periphery | 0.000521 | 0.002561 | 0.000218 | 0.003619 | 0.000799 | 0.001045 | 0.000122 | 0.000960 | 0.000635 | 0.001748 | 0.000192 | 0.001963 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -9.28 | -15.54 | -2.58 | -1.50 | -0.53 | -1.49 | -0.98 | -3.32 | 0.53 | -2.49 | -2.28 | -13.10 | -3.98 | -0.30 | -0.45 |
Bud Neck | 0.18 | 5.71 | 7.03 | 7.15 | 0.73 | -10.39 | 7.30 | -2.07 | 9.40 | -11.28 | -6.71 | 6.37 | 4.72 | 11.51 | -2.75 |
Bud Site | -6.14 | -3.25 | -6.25 | -2.73 | -2.08 | -9.16 | -0.38 | -10.06 | -3.84 | -7.77 | -10.05 | -3.45 | -11.14 | -4.61 | -4.00 |
Cell Periphery | -3.97 | 2.49 | 1.35 | 4.76 | -0.98 | -0.69 | -4.95 | 5.02 | 5.77 | 5.66 | -3.23 | -3.97 | 5.64 | 7.84 | 5.59 |
Cytoplasm | 13.06 | 50.96 | 22.06 | 7.63 | -23.16 | 3.16 | 17.04 | 8.40 | 5.17 | -12.32 | 12.00 | 49.94 | 21.81 | 8.37 | -31.10 |
Cytoplasmic Foci | 10.30 | 38.81 | 36.73 | 22.99 | -8.17 | 4.78 | 23.11 | 28.27 | 27.37 | 5.83 | 10.90 | 45.85 | 46.61 | 35.84 | 0.04 |
Eisosomes | 6.33 | 9.38 | 10.48 | 4.12 | 2.53 | 4.96 | -3.69 | 12.20 | 9.06 | 4.59 | 8.03 | -0.37 | 14.25 | 8.56 | 5.10 |
Endoplasmic Reticulum | -6.62 | -19.70 | -16.30 | -8.70 | 11.40 | -1.93 | -4.72 | -8.03 | -5.67 | 0.28 | -5.94 | -19.54 | -17.62 | -9.54 | 13.22 |
Endosome | -1.56 | 8.52 | 7.00 | 6.87 | -0.19 | -4.05 | 4.56 | 6.67 | 8.69 | 0.38 | -3.72 | 9.80 | 10.30 | 11.26 | 0.99 |
Golgi | -2.97 | 6.58 | 0.56 | 2.74 | -2.00 | -1.04 | 3.11 | 2.00 | 4.18 | -2.12 | -2.97 | 5.98 | 2.26 | 4.87 | -2.79 |
Lipid Particles | 6.85 | 10.16 | 11.27 | 9.97 | 3.52 | 3.07 | 7.32 | 9.24 | 8.30 | 4.90 | 6.56 | 12.53 | 14.84 | 11.73 | 7.24 |
Mitochondria | -3.28 | 4.11 | 1.22 | 4.11 | -2.25 | -3.66 | 4.71 | 1.96 | 6.03 | -7.02 | -5.09 | 6.01 | 2.32 | 7.30 | -5.24 |
Mitotic Spindle | -4.10 | -2.70 | -6.27 | -3.49 | -3.65 | -5.14 | 1.46 | -10.52 | -5.44 | -10.68 | -6.32 | -2.78 | -11.92 | -6.64 | -8.14 |
None | -1.71 | 3.25 | 2.09 | 4.88 | -2.21 | 2.55 | 1.08 | 4.46 | 3.04 | 2.02 | 0.84 | 3.59 | 4.29 | 4.71 | -0.14 |
Nuclear Periphery | -4.05 | -22.41 | -20.44 | -12.96 | 4.92 | 0.10 | -3.49 | -18.40 | -21.19 | -14.51 | -3.23 | -20.86 | -29.87 | -23.86 | 0.78 |
Nuclear Periphery Foci | -2.01 | -6.18 | -5.38 | -3.68 | 1.78 | 1.28 | 1.01 | -0.07 | -1.31 | -1.05 | -0.95 | -5.62 | -3.43 | -2.95 | 2.87 |
Nucleolus | -4.09 | 2.22 | 5.40 | 8.19 | 6.31 | -1.30 | 4.93 | 6.43 | 8.12 | 2.24 | -4.05 | 5.10 | 8.22 | 11.44 | 6.14 |
Nucleus | -15.28 | -55.77 | -25.87 | -8.17 | 25.57 | -0.25 | -21.62 | -10.49 | -10.45 | 16.11 | -11.61 | -55.68 | -27.19 | -12.84 | 33.89 |
Peroxisomes | -6.07 | 11.50 | 10.31 | 9.84 | -2.35 | -4.72 | 7.21 | 5.28 | 10.27 | -1.84 | -8.06 | 12.38 | 8.61 | 13.91 | -2.18 |
Vacuole | -2.29 | -7.91 | -19.35 | -17.98 | -10.46 | -6.44 | -4.79 | -24.70 | -20.03 | -8.01 | -5.14 | -9.74 | -30.28 | -26.85 | -13.05 |
Vacuole Periphery | -3.12 | 6.72 | -1.31 | 1.03 | -1.68 | -1.27 | 3.85 | 1.85 | 4.69 | -3.22 | -3.53 | 5.66 | -0.69 | 2.13 | -2.19 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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