Standard name
Human Ortholog
Description CDK inhibitor and nuclear anchor; during the cell cycle Far1p sequesters the GEF Cdc24p in the nucleus; phosphorylation by Cdc28p-Cln results in SCFCdc4 complex-mediated ubiquitin-dependent degradation, releasing Cdc24p for export and activation of GTPase Cdc42p; in response to pheromone, phosphorylation of Far1p by MAPK Fus3p results in association with, and inhibition of Cdc28p-Cln, as well as Msn5p mediated nuclear export of Far1p-Cdc24p, targeting Cdc24p to polarity sites
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0.05 0 0.06 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0
Cytoplasm 0.7 0.82 0.89 0.84 0.95 0.86 0.91 0.88 0.75 0.69 0.76 0.73 0.84 0.91 0.98 0.83 0.87 0.81 0.46 0.6 0.6 0.56 0.66 0.57
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0.08 0.19 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0.06 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.06 0 0 0.14 0 0.18 0.12 0.14 0.31 0.37 0.37 0.34 0 0 0 0 0.1 0 0 0 0.05 0.07 0.06 0
Nucleus 0.18 0.11 0.06 0.11 0 0 0 0 0.05 0.07 0 0 0.07 0 0 0 0 0.06 0.2 0.12 0.1 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0.08 0.06 0 0 0 0
Vac/Vac Membrane 0.13 0.1 0.06 0 0 0 0 0 0 0 0 0 0.09 0.08 0 0.09 0.05 0.06 0.08 0.07 0.1 0.13 0.11 0.16
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 3 3 2 9 11 12
Bud 0 1 0 0 0 2 3 1 6 5 7 2 3 0 1 0 0 0 24 15 1 20 11 17
Bud Neck 0 3 3 0 2 1 3 6 5 3 0 7 0 0 0 1 0 0 5 5 0 8 3 12
Bud Site 0 0 0 0 0 0 1 0 1 2 3 3 0 0 0 0 0 0 – – – – – –
Cell Periphery 1 2 2 1 0 0 0 0 0 0 1 0 1 0 0 0 0 1 1 0 2 1 1 2
Cytoplasm 227 248 126 53 145 81 190 130 122 131 147 124 369 431 549 53 34 13 163 190 85 181 245 229
Endoplasmic Reticulum 2 1 0 0 0 0 0 0 0 0 0 0 0 1 2 5 3 3 0 0 2 5 6 4
Endosome 1 0 0 0 0 0 0 0 1 0 0 1 2 2 2 4 0 1 7 2 0 4 1 3
Golgi 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 1 0 2 5 1
Mitochondria 19 6 0 9 2 17 25 21 51 70 72 57 17 7 4 2 4 0 12 9 6 22 22 13
Nucleus 59 34 9 7 4 2 5 5 8 13 5 6 31 15 0 1 0 1 69 37 13 11 6 17
Nuclear Periphery 1 1 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 1 0
Nucleolus 0 0 0 0 0 0 0 2 0 1 2 0 1 0 0 0 0 0 0 2 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 1
SpindlePole 1 0 1 0 0 0 2 1 0 3 1 1 0 0 1 1 2 0 28 18 4 5 7 11
Vac/Vac Membrane 41 29 9 0 2 1 2 2 3 3 2 5 38 39 11 6 2 1 27 22 13 42 40 62
Unique Cell Count 323 304 141 63 152 94 209 148 162 189 193 169 440 472 561 64 39 16 353 319 142 325 372 400
Labelled Cell Count 352 326 150 70 155 104 231 168 197 231 240 206 462 496 572 76 45 20 353 319 142 325 372 400


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.4 4.2 5.1 4.1 4.8 3.6 4.0 4.0 3.4 3.2 2.9 3.4 4.2 4.2 4.2 5.9 5.5 5.5 4.9 4.9 5.0
Std Deviation (1e-4) 0.9 1.0 1.6 1.2 1.6 1.7 1.5 1.3 1.2 1.2 0.8 1.3 1.1 1.5 1.3 1.7 1.7 1.5 1.7 1.3 1.3
Intensity Change (Log2) – – – -0.32 -0.11 -0.53 -0.37 -0.35 -0.6 -0.69 -0.81 -0.58 -0.3 -0.29 -0.29 0.21 0.1 0.1 -0.07 -0.07 -0.02


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 3.6151 2.5469 1.8802 1.2839 1.7906 2.6886 1.7273 0.7061 0.1326 -0.0519 -0.5404 0.7519 1.3347 0.1146 -0.3964 -0.22 -0.6986 0.4621
Actin 0.0003 0.0005 0.0005 0.0002 0.0004 0.0006 0.0004 0.0007 0.0013 0.0004 0.0009 0.0011 0.0002 0.0007 0.0008 0.0006 0.0005 0.0009
Bud 0.0007 0.002 0.0031 0.0026 0.0064 0.0009 0.0023 0.0136 0.0081 0.0006 0.0099 0.0011 0.0011 0.005 0.005 0.0038 0.0017 0.0012
Bud Neck 0.0008 0.001 0.0013 0.0012 0.0024 0.0059 0.0013 0.0014 0.0026 0.0005 0.0033 0.0066 0.0009 0.0012 0.0016 0.0009 0.0012 0.0046
Bud Periphery 0.001 0.0014 0.0027 0.0028 0.011 0.0012 0.0022 0.0043 0.008 0.0006 0.0118 0.001 0.0008 0.0027 0.0045 0.0037 0.0017 0.0011
Bud Site 0.0013 0.0062 0.0027 0.0016 0.002 0.0008 0.0074 0.0177 0.0123 0.0002 0.0029 0.0006 0.0011 0.0082 0.0048 0.0011 0.0006 0.0003
Cell Periphery 0.0002 0.0002 0.0002 0.0001 0.0004 0.0001 0.0003 0.0004 0.0004 0 0.0003 0.0001 0.0001 0.0002 0.0002 0.0001 0.0001 0.0001
Cytoplasm 0.263 0.2262 0.1358 0.0906 0.1274 0.2162 0.2729 0.3012 0.2645 0.2805 0.2291 0.2613 0.2363 0.1944 0.1608 0.1824 0.2022 0.212
Cytoplasmic Foci 0.0038 0.0042 0.0056 0.0038 0.0066 0.0068 0.0051 0.0047 0.0101 0.0047 0.0168 0.0071 0.0034 0.0056 0.0052 0.0049 0.0063 0.0045
Eisosomes 0 0.0001 0.0001 0 0.0001 0 0.0001 0 0.0001 0 0.0001 0 0 0.0001 0.0001 0 0 0
Endoplasmic Reticulum 0.0039 0.0034 0.0023 0.0006 0.003 0.002 0.0028 0.0043 0.0036 0.0024 0.0036 0.0027 0.0026 0.003 0.0022 0.0029 0.003 0.0018
Endosome 0.0018 0.0018 0.0017 0.0006 0.0034 0.0021 0.0025 0.0034 0.0034 0.0024 0.0045 0.0028 0.0017 0.0025 0.0018 0.0026 0.0035 0.0021
Golgi 0.0001 0.0001 0.0001 0 0.0001 0.0003 0.0001 0.0002 0.0003 0.0001 0.0003 0.0001 0.0001 0.0002 0.0001 0.0001 0.0001 0.0001
Lipid Particles 0.0002 0.0001 0.0003 0.0002 0.0003 0.0002 0.0003 0.0001 0.0003 0.0001 0.0005 0.0001 0.0002 0.0002 0.0002 0.0002 0.0005 0.0001
Mitochondria 0.0006 0.001 0.0012 0.0007 0.0015 0.0008 0.0005 0.0017 0.0019 0.0011 0.0019 0.0008 0.0005 0.001 0.0011 0.0014 0.0013 0.0006
None 0.2781 0.4697 0.6892 0.8328 0.6906 0.3413 0.2066 0.3134 0.4842 0.5685 0.4919 0.2198 0.2879 0.4581 0.6321 0.5752 0.5893 0.3461
Nuclear Periphery 0.0075 0.0143 0.0041 0.0014 0.0054 0.0053 0.0083 0.009 0.0081 0.0074 0.0099 0.0081 0.0087 0.0079 0.0069 0.0102 0.0137 0.0065
Nucleolus 0.0025 0.0035 0.0041 0.0023 0.005 0.0041 0.0023 0.0059 0.0041 0.0011 0.0049 0.0037 0.0016 0.0035 0.0036 0.0034 0.0058 0.0034
Nucleus 0.4226 0.2545 0.1331 0.0519 0.1116 0.386 0.4681 0.2971 0.163 0.1207 0.1747 0.4473 0.4371 0.2807 0.1495 0.1852 0.1453 0.3852
Peroxisomes 0.0001 0.0002 0.0003 0.0002 0.0005 0.0004 0.0001 0.0003 0.0006 0.0003 0.0012 0.0003 0.0001 0.0006 0.0003 0.0004 0.0004 0.0002
Punctate Nuclear 0.0078 0.0062 0.0087 0.0049 0.0093 0.023 0.0123 0.0156 0.0168 0.0057 0.021 0.0327 0.0124 0.02 0.0159 0.0166 0.0184 0.0275
Vacuole 0.0034 0.0028 0.0027 0.0011 0.011 0.0017 0.0035 0.0044 0.0054 0.0021 0.0088 0.0024 0.0027 0.0037 0.0029 0.0035 0.0036 0.0015
Vacuole Periphery 0.0004 0.0005 0.0004 0.0002 0.0015 0.0003 0.0004 0.0006 0.0008 0.0005 0.0016 0.0004 0.0004 0.0005 0.0005 0.0007 0.0009 0.0003

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 5.0899 5.5392 42.5714 85.4723 22.6671 7.9624 7.801 16.0654 47.7086 23.5309
Translational Efficiency 0.4033 0.5872 0.7103 0.6567 0.6339 0.4172 0.5944 0.6214 0.738 0.7544

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✔
Standard name
Human Ortholog
Description CDK inhibitor and nuclear anchor; during the cell cycle Far1p sequesters the GEF Cdc24p in the nucleus; phosphorylation by Cdc28p-Cln results in SCFCdc4 complex-mediated ubiquitin-dependent degradation, releasing Cdc24p for export and activation of GTPase Cdc42p; in response to pheromone, phosphorylation of Far1p by MAPK Fus3p results in association with, and inhibition of Cdc28p-Cln, as well as Msn5p mediated nuclear export of Far1p-Cdc24p, targeting Cdc24p to polarity sites
Localization
Cell Percentages cytoplasm (15%), nucleus (10%), mixed (46%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Far1

Far1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Far1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available