Standard name
Human Ortholog
Description Mannose-containing glycoprotein constituent of the cell wall; member of the PIR (proteins with internal repeats) family
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.59 0.67 0.74 0.77 0.5 0.64 0.69 0.61 – 0.71 0.74 0.78 0.88 0.92 0.97 0.59 0.59 0.61 0.11 0.15 0.16 0.18 0.12 0.14
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0.06 0 0.05 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0.06 0 0 0.1 0.11 0.15 – 0.13 0.17 0.2 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0.05 0.09 0.09 0.05 0.06 – 0 0 0 0 0 0 0.15 0.14 0.14 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.44 0.4 0.32 0.41 0.57 0.43 0.35 0.33 – 0.3 0.28 0.21 0.11 0.08 0 0.41 0.45 0.46 0.77 0.75 0.71 0.71 0.72 0.69
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 – 0 2 0 0 0 0 0 0 0 0 2 4 0 0 3
Bud 0 0 2 0 0 0 7 8 – 7 11 7 1 0 0 0 0 0 12 9 7 5 13 7
Bud Neck 1 0 0 0 0 0 0 0 – 0 0 0 2 0 1 0 0 0 0 1 0 1 2 2
Bud Site 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 1 0 0 0 0 0 0 – 1 0 0 2 1 1 1 0 0 0 0 0 0 0 0
Cytoplasm 305 342 271 190 180 292 396 378 – 505 395 509 330 365 446 217 176 157 60 81 62 42 38 43
Endoplasmic Reticulum 2 0 0 0 0 1 4 0 – 2 2 3 7 2 4 22 9 14 2 2 3 1 3 5
Endosome 20 3 7 0 14 0 0 2 – 3 0 1 1 3 1 4 7 3 26 13 8 3 5 10
Golgi 3 0 0 0 0 0 0 0 – 0 0 0 0 0 0 2 0 0 3 1 1 1 0 3
Mitochondria 21 12 22 6 16 44 65 92 – 91 90 131 0 0 0 9 2 1 7 6 11 6 10 14
Nucleus 23 22 11 12 32 40 27 36 – 27 18 18 6 2 1 55 43 35 4 2 0 1 1 0
Nuclear Periphery 0 0 0 3 10 4 10 8 – 3 3 4 0 0 0 0 1 0 1 0 0 0 2 0
Nucleolus 0 0 2 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 – 1 0 0 0 0 0 0 0 0 0 1 2 0 0 0
SpindlePole 0 0 1 0 0 0 0 2 – 1 1 2 0 0 0 2 0 0 8 6 1 0 2 3
Vac/Vac Membrane 226 208 116 102 205 196 201 204 – 216 151 135 42 33 10 152 135 118 438 409 276 166 225 220
Unique Cell Count 515 514 368 246 362 458 574 619 711 535 656 374 398 458 367 299 259 570 546 387 234 313 321
Labelled Cell Count 601 588 432 313 457 577 710 730 857 673 810 391 406 464 464 373 328 570 546 387 234 313 321


Vacuole

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.7 4.6 4.3 4.4 4.9 4.8 4.1 4.2 – 4.2 4.2 4.1 5.5 5.2 5.1 6.0 6.0 6.1 6.3 6.3 6.2
Std Deviation (1e-4) 0.8 0.9 1.4 1.6 1.9 1.6 1.5 1.1 – 1.3 1.3 1.2 1.0 1.0 1.0 1.3 1.5 1.4 1.5 1.7 1.7
Intensity Change (Log2) – – – 0.02 0.18 0.14 -0.06 -0.03 – -0.02 -0.02 -0.07 0.36 0.28 0.25 0.47 0.49 0.5 0.55 0.56 0.53


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – 0 0 0
Bud Neck – – – – – – – – – – – – 0 0 0
Bud Site – – – – – – – – – – – – 0 0 0
Cell Periphery – – – – – – – – – – – – 0 0 0
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – 0 0 0
Endosome – – – – – – – – – – – – 0 0 0
Golgi – – – – – – – – – – – – 0 0 0
Mitochondria – – – – – – – – – – – – 0 0 0
Nuclear Periphery – – – – – – – – – – – – 0 0 0
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – 0 0 0
Peroxisomes – – – – – – – – – – – – 0 0 0
SpindlePole – – – – – – – – – – – – 0 0 0
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – 0 0 0
Cytoplasm – – – – – – – – – – – – 0 0 0
Nucleus – – – – – – – – – – – – 0 0 0
Vacuole – – – – – – – – – – – – 2.8 3.6 3.6

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 0.8598 1.3246 1.2316 2.6727 1.1207 1.315 1.5033 3.5896 3.0734 3.4916 2.6211 3.2222 0.25 2.4878 2.0944 2.2814 2.6098 1.9753
Actin 0.0089 0 0.0016 0.0019 0.0173 0.0001 0.0021 0.0002 0.0003 0.0001 0.0001 0.0056 0 0 0 0 0 0
Bud 0.001 0.0016 0.0033 0.0009 0.0007 0.0007 0.0088 0.0006 0.0054 0.0008 0.0057 0.0006 0.0004 0.0007 0.0005 0.0006 0.0005 0.0007
Bud Neck 0.0037 0.002 0.0022 0.0026 0.0045 0.0028 0.0006 0.0035 0.0009 0.0003 0.002 0.0023 0.0008 0.0019 0.0015 0.001 0.0019 0.0019
Bud Periphery 0.0022 0.0033 0.0057 0.0026 0.0014 0.0024 0.0064 0.0013 0.0071 0.0018 0.0133 0.0016 0.0011 0.0015 0.0011 0.0012 0.001 0.0021
Bud Site 0.0027 0.0051 0.0059 0.0027 0.002 0.001 0.0037 0.0049 0.0055 0.0007 0.0063 0.001 0.0009 0.0021 0.0015 0.0015 0.0023 0.0014
Cell Periphery 0.0014 0.0017 0.0016 0.0013 0.001 0.002 0.0024 0.0027 0.0019 0.0007 0.0013 0.0025 0.0009 0.0018 0.0013 0.001 0.0016 0.0014
Cytoplasm 0.0486 0.046 0.0334 0.0249 0.0245 0.0312 0.0292 0.0584 0.0342 0.0147 0.0088 0.0229 0.0356 0.0297 0.0234 0.0189 0.0129 0.0229
Cytoplasmic Foci 0.0546 0.0408 0.0454 0.0627 0.0223 0.0282 0.0309 0.0559 0.0495 0.073 0.0051 0.0397 0.0289 0.0122 0.0366 0.0093 0.0295 0.0191
Eisosomes 0.0003 0.0001 0.0001 0 0.0002 0 0.0002 0 0 0.0003 0.0007 0.0001 0 0 0 0 0 0
Endoplasmic Reticulum 0.0102 0.004 0.0047 0.0032 0.0038 0.0035 0.0047 0.0027 0.0047 0.0012 0.0025 0.0047 0.0055 0.005 0.0028 0.001 0.0017 0.0032
Endosome 0.2595 0.1428 0.2866 0.2945 0.1975 0.2314 0.2064 0.2297 0.2156 0.2097 0.1589 0.2018 0.285 0.1541 0.2846 0.2349 0.249 0.2048
Golgi 0.0087 0.0021 0.0063 0.0051 0.0072 0.0043 0.0045 0.0056 0.0046 0.0024 0.0022 0.0047 0.0037 0.0017 0.008 0.0059 0.0055 0.0023
Lipid Particles 0.0396 0.0092 0.0248 0.0069 0.0181 0.0185 0.0534 0.0123 0.0263 0.0088 0.0022 0.0236 0.0203 0.0035 0.0214 0.0114 0.0107 0.0071
Mitochondria 0.0073 0.0051 0.0205 0.0093 0.003 0.012 0.0181 0.0025 0.022 0.017 0.0275 0.007 0.0079 0.0026 0.0076 0.0025 0.0099 0.0029
None 0.1346 0.1417 0.1048 0.0836 0.1193 0.0316 0.1725 0.1586 0.0733 0.2385 0.1644 0.1261 0.0807 0.0341 0.0402 0.0284 0.0177 0.0176
Nuclear Periphery 0.0177 0.0085 0.0065 0.0054 0.0166 0.0093 0.0068 0.0028 0.0053 0.0027 0.0032 0.005 0.0067 0.0069 0.0094 0.0018 0.0022 0.0075
Nucleolus 0.0041 0.0082 0.0059 0.0041 0.0145 0.0078 0.0038 0.007 0.0026 0.0042 0.0018 0.0056 0.0031 0.0048 0.0044 0.002 0.0139 0.0042
Nucleus 0.0183 0.0187 0.0118 0.0121 0.0135 0.0274 0.0043 0.0074 0.004 0.0027 0.0047 0.0064 0.0071 0.0095 0.01 0.0028 0.0082 0.0077
Peroxisomes 0.0101 0.0013 0.0043 0.0061 0.0069 0.0022 0.0038 0.0013 0.0063 0.0025 0.0007 0.0031 0.0014 0.0004 0.0019 0.001 0.0019 0.0007
Punctate Nuclear 0.0038 0.004 0.0016 0.0035 0.0057 0.004 0.004 0.001 0.0036 0.0012 0.0001 0.0022 0.0014 0.0003 0.0027 0.0001 0.0005 0.0008
Vacuole 0.3097 0.5027 0.3747 0.4201 0.466 0.5206 0.3807 0.398 0.4559 0.359 0.5098 0.4613 0.4147 0.6401 0.4711 0.5982 0.5672 0.5953
Vacuole Periphery 0.053 0.0512 0.0483 0.0464 0.0539 0.059 0.0526 0.0435 0.071 0.0577 0.0788 0.0722 0.0937 0.087 0.0701 0.0764 0.0619 0.0963

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 584.7721 829.1905 653.818 517.54 695.4364 469.171 858.4422 682.4754 508.5568 652.0627
Translational Efficiency 2.8758 3.0213 2.101 1.777 2.5287 3.2572 2.6521 2.7611 2.2745 2.5213

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
2494 1768 1645 1419 1711 2307 509 1718 4205 4075 2154 3137

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 662.60 775.01 713.24 828.42 685.61 719.53 759.43 861.52 671.96 743.60 724.15 846.55
Standard Deviation 79.54 110.16 106.67 138.53 84.60 92.73 89.27 127.84 82.42 104.35 104.68 133.80
Intensity Change Log 2 — 0.226077 0.106249 0.322224 — 0.069667 0.147529 0.329496 — 0.148656 0.127389 0.325927

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000046 0.000114 0.000124 0.001203 0.000050 0.000084 0.000290 0.001033 0.000048 0.000097 0.000163 0.001110
Bud Neck 0.050386 0.035721 0.033245 0.026957 0.030805 0.030166 0.038638 0.035518 0.042419 0.032576 0.034520 0.031645
Bud Site 0.018598 0.020143 0.013592 0.051526 0.010486 0.020715 0.030755 0.052053 0.015297 0.020467 0.017647 0.051815
Cell Periphery 0.001121 0.000187 0.000196 0.000235 0.000501 0.000351 0.000085 0.000323 0.000869 0.000279 0.000170 0.000283
Cytoplasm 0.107427* 0.195902* 0.293544* 0.281557* 0.218150* 0.077130 0.421508* 0.319182* 0.152480* 0.128661* 0.323783* 0.302162*
Cytoplasmic Foci 0.034565 0.081658 0.024711 0.058610 0.069000 0.045863 0.042614 0.105936 0.048576 0.061393 0.028942 0.084528
Eisosomes 0.000097 0.000045 0.000057 0.000045 0.000062 0.000039 0.000042 0.000042 0.000082 0.000042 0.000054 0.000044
Endoplasmic Reticulum 0.003105 0.009831 0.002677 0.001491 0.000798 0.001516 0.001377 0.000783 0.002166 0.005124 0.002370 0.001104
Endosome 0.092404 0.090951 0.040596 0.088744 0.063447 0.110938 0.050574 0.109765 0.080621 0.102267 0.042954 0.100257
Golgi 0.001580 0.002621 0.000513 0.005868 0.000803 0.003289 0.000409 0.004996 0.001264 0.002999 0.000488 0.005390
Lipid Particles 0.003420 0.002851 0.002802 0.003261 0.003824 0.002348 0.001851 0.003768 0.003585 0.002566 0.002577 0.003539
Mitochondria 0.002616 0.004835 0.001366 0.002378 0.003005 0.006488 0.001120 0.001724 0.002774 0.005770 0.001308 0.002020
Mitotic Spindle 0.000054 0.004450 0.004604 0.038327 0.001186 0.001990 0.010443 0.021376 0.000514 0.003058 0.005984 0.029044
None 0.000788 0.000968 0.006281 0.002713 0.000685 0.000386 0.002184 0.000993 0.000746 0.000638 0.005313 0.001771
Nuclear Periphery 0.000120 0.000255 0.002175 0.001061 0.000287 0.000292 0.001233 0.000515 0.000188 0.000276 0.001952 0.000762
Nuclear Periphery Foci 0.000083 0.000431 0.001368 0.003195 0.000233 0.000115 0.001891 0.001555 0.000144 0.000252 0.001492 0.002297
Nucleolus 0.000612 0.001397 0.006928 0.001632 0.000868 0.000763 0.002497 0.000752 0.000716 0.001038 0.005881 0.001150
Nucleus 0.011415 0.027792 0.319027* 0.046386 0.035686 0.007603 0.156761* 0.031828 0.021290 0.016363 0.280683* 0.038413
Peroxisomes 0.001600 0.004591 0.002412 0.013730 0.002307 0.004051 0.006104 0.035088 0.001888 0.004285 0.003284 0.025427
Vacuole 0.657434* 0.505281* 0.241163* 0.366630* 0.553494* 0.670031* 0.228674* 0.270831* 0.615141* 0.598552* 0.238212* 0.314165*
Vacuole Periphery 0.012529 0.009977 0.002619 0.004451 0.004324 0.015841 0.000951 0.001938 0.009191 0.013297 0.002225 0.003075

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -3.00 -6.60 -4.57 -4.13 -3.95 -2.08 -3.60 -7.77 -7.32 -4.13 -3.68 -6.76 -8.13 -7.52 -6.51
Bud Neck 4.97 5.59 9.76 5.25 3.78 0.15 -1.65 0.21 0.08 1.78 4.97 3.16 7.81 3.50 3.28
Bud Site -0.90 3.12 -5.95 -5.27 -7.41 -6.65 -3.38 -11.23 -6.61 -3.39 -4.61 -0.22 -11.55 -8.41 -9.59
Cell Periphery 9.75 9.92 9.98 1.20 0.78 0.41 1.18 0.81 1.66 -1.51 3.80 4.66 4.30 1.95 -0.63
Cytoplasm -12.90 -22.98 -23.36 -10.90 -1.21 20.69 -12.07 -12.22 -32.38 4.00 4.97 -22.57 -26.85 -30.76 -0.69
Cytoplasmic Foci -13.08 3.75 -4.54 8.86 -7.82 6.94 6.64 -3.80 -10.28 -9.53 -5.36 9.50 -7.73 -2.23 -15.78
Eisosomes 7.43 5.83 7.32 -0.74 3.19 5.98 3.92 5.80 -0.85 1.16 9.26 6.40 8.99 -1.18 4.26
Endoplasmic Reticulum -3.14 0.60 5.60 3.88 7.36 -5.59 -6.24 -1.22 4.75 5.50 -3.15 -2.49 5.82 4.22 10.71
Endosome 0.45 14.11 9.19 8.12 -2.80 -11.02 4.54 -1.46 8.25 -5.21 -6.63 14.08 6.03 11.59 -6.76
Golgi -3.06 5.98 -1.44 -0.13 -2.84 -6.58 2.94 -3.45 0.78 -4.42 -6.71 5.91 -3.13 0.46 -4.89
Lipid Particles 1.82 1.66 0.72 -0.84 -0.86 2.61 2.94 0.23 -3.85 -4.46 3.45 2.74 0.42 -3.53 -2.64
Mitochondria -3.47 3.96 2.79 4.89 -0.62 -3.32 2.29 1.87 7.37 -1.50 -5.15 3.74 3.27 8.81 -0.92
Mitotic Spindle -4.41 -5.13 -10.14 -8.53 -7.87 -0.99 -3.42 -9.06 -8.67 -3.86 -4.24 -5.90 -13.72 -12.09 -9.27
None -0.65 -6.27 -3.56 -3.41 4.16 1.74 -7.07 -2.78 -4.49 5.46 0.65 -7.15 -4.11 -5.20 5.55
Nuclear Periphery -4.27 -11.85 -12.45 -11.13 3.58 -0.02 -10.54 -10.39 -4.58 4.74 -1.07 -13.47 -16.62 -9.80 5.94
Nuclear Periphery Foci -2.39 -5.57 -6.44 -5.40 -1.29 3.43 -6.37 -6.74 -7.29 2.09 -1.65 -7.15 -9.13 -8.41 0.02
Nucleolus -3.11 -9.71 -6.26 -1.04 8.01 0.55 -4.66 -1.80 -1.81 4.35 -2.16 -10.65 -6.11 -2.04 9.40
Nucleus -9.34 -42.41 -19.57 -12.86 31.38 14.02 -15.28 -3.71 -17.68 14.05 4.01 -43.12 -18.23 -21.05 35.87
Peroxisomes -8.16 -2.46 -6.08 -3.28 -5.11 -3.11 -2.71 -10.37 -9.26 -7.25 -7.09 -3.30 -12.05 -9.70 -10.39
Vacuole 15.30 46.18* 25.06 8.81 -15.81 -11.55 21.57 21.06 35.60* -6.26 2.33 49.23* 34.75 31.36 -15.94
Vacuole Periphery 3.54 17.64 15.69 11.97 -0.12 -16.45 10.83 8.90 22.81 -3.31 -7.34 18.80 18.85 25.13 0.74
Standard name
Human Ortholog
Description Mannose-containing glycoprotein constituent of the cell wall; member of the PIR (proteins with internal repeats) family
Localization
Cell Percentages vacuole (81%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Cis3

Cis3


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Cis3-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available