Standard name | |
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Human Ortholog | |
Description | Mannose-containing glycoprotein constituent of the cell wall; member of the PIR (proteins with internal repeats) family |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 4 | 0 | 0 | 3 |
Bud | 0 | 0 | 2 | 0 | 0 | 0 | 7 | 8 | – | 7 | 11 | 7 | 1 | 0 | 0 | 0 | 0 | 0 | 12 | 9 | 7 | 5 | 13 | 7 |
Bud Neck | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 2 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 2 | 2 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | – | 1 | 0 | 0 | 2 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 305 | 342 | 271 | 190 | 180 | 292 | 396 | 378 | – | 505 | 395 | 509 | 330 | 365 | 446 | 217 | 176 | 157 | 60 | 81 | 62 | 42 | 38 | 43 |
Endoplasmic Reticulum | 2 | 0 | 0 | 0 | 0 | 1 | 4 | 0 | – | 2 | 2 | 3 | 7 | 2 | 4 | 22 | 9 | 14 | 2 | 2 | 3 | 1 | 3 | 5 |
Endosome | 20 | 3 | 7 | 0 | 14 | 0 | 0 | 2 | – | 3 | 0 | 1 | 1 | 3 | 1 | 4 | 7 | 3 | 26 | 13 | 8 | 3 | 5 | 10 |
Golgi | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 3 | 1 | 1 | 1 | 0 | 3 |
Mitochondria | 21 | 12 | 22 | 6 | 16 | 44 | 65 | 92 | – | 91 | 90 | 131 | 0 | 0 | 0 | 9 | 2 | 1 | 7 | 6 | 11 | 6 | 10 | 14 |
Nucleus | 23 | 22 | 11 | 12 | 32 | 40 | 27 | 36 | – | 27 | 18 | 18 | 6 | 2 | 1 | 55 | 43 | 35 | 4 | 2 | 0 | 1 | 1 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 3 | 10 | 4 | 10 | 8 | – | 3 | 3 | 4 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 2 | 0 |
Nucleolus | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 2 | – | 1 | 1 | 2 | 0 | 0 | 0 | 2 | 0 | 0 | 8 | 6 | 1 | 0 | 2 | 3 |
Vac/Vac Membrane | 226 | 208 | 116 | 102 | 205 | 196 | 201 | 204 | – | 216 | 151 | 135 | 42 | 33 | 10 | 152 | 135 | 118 | 438 | 409 | 276 | 166 | 225 | 220 |
Unique Cell Count | 515 | 514 | 368 | 246 | 362 | 458 | 574 | 619 | 711 | 535 | 656 | 374 | 398 | 458 | 367 | 299 | 259 | 570 | 546 | 387 | 234 | 313 | 321 | |
Labelled Cell Count | 601 | 588 | 432 | 313 | 457 | 577 | 710 | 730 | 857 | 673 | 810 | 391 | 406 | 464 | 464 | 373 | 328 | 570 | 546 | 387 | 234 | 313 | 321 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 4.7 | 4.6 | 4.3 | 4.4 | 4.9 | 4.8 | 4.1 | 4.2 | – | 4.2 | 4.2 | 4.1 | 5.5 | 5.2 | 5.1 | 6.0 | 6.0 | 6.1 | 6.3 | 6.3 | 6.2 |
Std Deviation (1e-4) | 0.8 | 0.9 | 1.4 | 1.6 | 1.9 | 1.6 | 1.5 | 1.1 | – | 1.3 | 1.3 | 1.2 | 1.0 | 1.0 | 1.0 | 1.3 | 1.5 | 1.4 | 1.5 | 1.7 | 1.7 |
Intensity Change (Log2) | – | – | – | 0.02 | 0.18 | 0.14 | -0.06 | -0.03 | – | -0.02 | -0.02 | -0.07 | 0.36 | 0.28 | 0.25 | 0.47 | 0.49 | 0.5 | 0.55 | 0.56 | 0.53 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | 2.8 | 3.6 | 3.6 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 0.8598 | 1.3246 | 1.2316 | 2.6727 | 1.1207 | 1.315 | 1.5033 | 3.5896 | 3.0734 | 3.4916 | 2.6211 | 3.2222 | 0.25 | 2.4878 | 2.0944 | 2.2814 | 2.6098 | 1.9753 |
Actin | 0.0089 | 0 | 0.0016 | 0.0019 | 0.0173 | 0.0001 | 0.0021 | 0.0002 | 0.0003 | 0.0001 | 0.0001 | 0.0056 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0.001 | 0.0016 | 0.0033 | 0.0009 | 0.0007 | 0.0007 | 0.0088 | 0.0006 | 0.0054 | 0.0008 | 0.0057 | 0.0006 | 0.0004 | 0.0007 | 0.0005 | 0.0006 | 0.0005 | 0.0007 |
Bud Neck | 0.0037 | 0.002 | 0.0022 | 0.0026 | 0.0045 | 0.0028 | 0.0006 | 0.0035 | 0.0009 | 0.0003 | 0.002 | 0.0023 | 0.0008 | 0.0019 | 0.0015 | 0.001 | 0.0019 | 0.0019 |
Bud Periphery | 0.0022 | 0.0033 | 0.0057 | 0.0026 | 0.0014 | 0.0024 | 0.0064 | 0.0013 | 0.0071 | 0.0018 | 0.0133 | 0.0016 | 0.0011 | 0.0015 | 0.0011 | 0.0012 | 0.001 | 0.0021 |
Bud Site | 0.0027 | 0.0051 | 0.0059 | 0.0027 | 0.002 | 0.001 | 0.0037 | 0.0049 | 0.0055 | 0.0007 | 0.0063 | 0.001 | 0.0009 | 0.0021 | 0.0015 | 0.0015 | 0.0023 | 0.0014 |
Cell Periphery | 0.0014 | 0.0017 | 0.0016 | 0.0013 | 0.001 | 0.002 | 0.0024 | 0.0027 | 0.0019 | 0.0007 | 0.0013 | 0.0025 | 0.0009 | 0.0018 | 0.0013 | 0.001 | 0.0016 | 0.0014 |
Cytoplasm | 0.0486 | 0.046 | 0.0334 | 0.0249 | 0.0245 | 0.0312 | 0.0292 | 0.0584 | 0.0342 | 0.0147 | 0.0088 | 0.0229 | 0.0356 | 0.0297 | 0.0234 | 0.0189 | 0.0129 | 0.0229 |
Cytoplasmic Foci | 0.0546 | 0.0408 | 0.0454 | 0.0627 | 0.0223 | 0.0282 | 0.0309 | 0.0559 | 0.0495 | 0.073 | 0.0051 | 0.0397 | 0.0289 | 0.0122 | 0.0366 | 0.0093 | 0.0295 | 0.0191 |
Eisosomes | 0.0003 | 0.0001 | 0.0001 | 0 | 0.0002 | 0 | 0.0002 | 0 | 0 | 0.0003 | 0.0007 | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0.0102 | 0.004 | 0.0047 | 0.0032 | 0.0038 | 0.0035 | 0.0047 | 0.0027 | 0.0047 | 0.0012 | 0.0025 | 0.0047 | 0.0055 | 0.005 | 0.0028 | 0.001 | 0.0017 | 0.0032 |
Endosome | 0.2595 | 0.1428 | 0.2866 | 0.2945 | 0.1975 | 0.2314 | 0.2064 | 0.2297 | 0.2156 | 0.2097 | 0.1589 | 0.2018 | 0.285 | 0.1541 | 0.2846 | 0.2349 | 0.249 | 0.2048 |
Golgi | 0.0087 | 0.0021 | 0.0063 | 0.0051 | 0.0072 | 0.0043 | 0.0045 | 0.0056 | 0.0046 | 0.0024 | 0.0022 | 0.0047 | 0.0037 | 0.0017 | 0.008 | 0.0059 | 0.0055 | 0.0023 |
Lipid Particles | 0.0396 | 0.0092 | 0.0248 | 0.0069 | 0.0181 | 0.0185 | 0.0534 | 0.0123 | 0.0263 | 0.0088 | 0.0022 | 0.0236 | 0.0203 | 0.0035 | 0.0214 | 0.0114 | 0.0107 | 0.0071 |
Mitochondria | 0.0073 | 0.0051 | 0.0205 | 0.0093 | 0.003 | 0.012 | 0.0181 | 0.0025 | 0.022 | 0.017 | 0.0275 | 0.007 | 0.0079 | 0.0026 | 0.0076 | 0.0025 | 0.0099 | 0.0029 |
None | 0.1346 | 0.1417 | 0.1048 | 0.0836 | 0.1193 | 0.0316 | 0.1725 | 0.1586 | 0.0733 | 0.2385 | 0.1644 | 0.1261 | 0.0807 | 0.0341 | 0.0402 | 0.0284 | 0.0177 | 0.0176 |
Nuclear Periphery | 0.0177 | 0.0085 | 0.0065 | 0.0054 | 0.0166 | 0.0093 | 0.0068 | 0.0028 | 0.0053 | 0.0027 | 0.0032 | 0.005 | 0.0067 | 0.0069 | 0.0094 | 0.0018 | 0.0022 | 0.0075 |
Nucleolus | 0.0041 | 0.0082 | 0.0059 | 0.0041 | 0.0145 | 0.0078 | 0.0038 | 0.007 | 0.0026 | 0.0042 | 0.0018 | 0.0056 | 0.0031 | 0.0048 | 0.0044 | 0.002 | 0.0139 | 0.0042 |
Nucleus | 0.0183 | 0.0187 | 0.0118 | 0.0121 | 0.0135 | 0.0274 | 0.0043 | 0.0074 | 0.004 | 0.0027 | 0.0047 | 0.0064 | 0.0071 | 0.0095 | 0.01 | 0.0028 | 0.0082 | 0.0077 |
Peroxisomes | 0.0101 | 0.0013 | 0.0043 | 0.0061 | 0.0069 | 0.0022 | 0.0038 | 0.0013 | 0.0063 | 0.0025 | 0.0007 | 0.0031 | 0.0014 | 0.0004 | 0.0019 | 0.001 | 0.0019 | 0.0007 |
Punctate Nuclear | 0.0038 | 0.004 | 0.0016 | 0.0035 | 0.0057 | 0.004 | 0.004 | 0.001 | 0.0036 | 0.0012 | 0.0001 | 0.0022 | 0.0014 | 0.0003 | 0.0027 | 0.0001 | 0.0005 | 0.0008 |
Vacuole | 0.3097 | 0.5027 | 0.3747 | 0.4201 | 0.466 | 0.5206 | 0.3807 | 0.398 | 0.4559 | 0.359 | 0.5098 | 0.4613 | 0.4147 | 0.6401 | 0.4711 | 0.5982 | 0.5672 | 0.5953 |
Vacuole Periphery | 0.053 | 0.0512 | 0.0483 | 0.0464 | 0.0539 | 0.059 | 0.0526 | 0.0435 | 0.071 | 0.0577 | 0.0788 | 0.0722 | 0.0937 | 0.087 | 0.0701 | 0.0764 | 0.0619 | 0.0963 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 584.7721 | 829.1905 | 653.818 | 517.54 | 695.4364 | 469.171 | 858.4422 | 682.4754 | 508.5568 | 652.0627 |
Translational Efficiency | 2.8758 | 3.0213 | 2.101 | 1.777 | 2.5287 | 3.2572 | 2.6521 | 2.7611 | 2.2745 | 2.5213 |
Dataset | Hit |
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Protein Concentration | ✔ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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