Standard name
Human Ortholog
Description Subunit of a Golgi mannosyltransferase complex; this complex also contains Anp1p, Mnn9p, Mnn10p, and Hoc1p, and mediates elongation of the polysaccharide mannan backbone; has homology to Mnn10p
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches – – 0 0 0 0 – 0 0 0 0 0 0.23 0.15 0.09 0 0 0 0 0 0 0 0 0
Bud – – 0 0.05 0.05 0 – 0.06 0.11 0.06 0.09 0.07 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck – – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site – – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery – – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm – – 0.1 0.09 0.08 0 – 0 0 0 0 0 0.08 0.26 0.58 0.68 0.35 0.41 0 0.05 0 0 0 0
Endoplasmic Reticulum – – 0 0 0 0 – 0 0 0 0 0 0.31 0.15 0.11 0.05 0 0 0 0 0 0 0 0
Endosome – – 0.4 0.52 0.49 0.3 – 0.21 0.06 0.06 0 0.08 0.19 0.22 0.12 0.05 0.25 0.25 0.22 0.28 0.4 0.38 0.33 0.23
Golgi – – 0.13 0.18 0.19 0.09 – 0 0 0 0 0 0.08 0.05 0 0 0.05 0.06 0.25 0 0.38 0.25 0.24 0.14
Mitochondria – – 0.3 0.29 0.22 0.57 – 0.58 0.81 0.84 0.85 0.83 0.13 0.07 0 0.05 0.1 0.1 0.14 0.24 0.05 0.15 0.11 0.09
Nucleus – – 0 0 0 0 – 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0 0
Nuclear Periphery – – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus – – 0 0 0 0.05 – 0.09 0.06 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes – – 0 0 0.08 0.08 – 0.21 0.13 0.1 0.09 0.14 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole – – 0 0.08 0.09 0.06 – 0.14 0.08 0.06 0.08 0.07 0.13 0.15 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane – – 0.27 0.09 0.05 0 – 0 0 0 0 0 0.06 0.06 0.07 0.4 0.44 0.46 0.28 0.29 0.1 0.12 0.23 0.46
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches – – 1 6 15 4 – 10 8 2 4 7 14 27 15 0 1 2 0 1 5 0 2 2
Bud – – 5 12 20 18 – 35 46 39 33 38 0 4 3 0 1 3 0 0 0 0 0 1
Bud Neck – – 0 0 0 0 – 0 0 0 1 0 0 2 4 0 0 0 0 0 0 0 0 0
Bud Site – – 0 0 0 0 – 5 4 4 5 6 0 0 0 0 0 0 – – – – – –
Cell Periphery – – 0 0 0 0 – 0 2 1 1 0 0 2 3 0 1 0 0 0 0 0 0 0
Cytoplasm – – 32 21 31 13 – 15 8 4 6 6 5 46 94 43 55 58 1 2 0 0 3 2
Endoplasmic Reticulum – – 12 3 10 4 – 2 3 2 1 2 19 26 17 3 5 5 0 0 1 2 3 7
Endosome – – 128 125 182 145 – 135 24 38 16 42 12 40 19 3 39 36 15 13 159 32 75 60
Golgi – – 43 44 71 43 – 22 9 8 4 6 5 9 2 0 8 9 18 1 150 21 56 37
Mitochondria – – 96 70 81 275 – 364 347 545 323 433 8 13 4 3 16 14 9 10 20 12 26 23
Nucleus – – 0 0 0 0 – 2 0 0 0 0 0 1 2 4 0 0 2 0 0 0 0 0
Nuclear Periphery – – 3 0 1 1 – 3 2 5 1 5 0 0 0 0 0 0 0 0 0 1 0 0
Nucleolus – – 1 7 5 26 – 56 26 41 13 20 0 0 0 0 1 1 0 0 0 0 0 0
Peroxisomes – – 5 4 28 37 – 135 55 66 34 75 0 0 0 0 0 0 1 0 13 1 7 1
SpindlePole – – 13 20 32 29 – 87 35 40 31 35 8 27 6 0 4 3 0 0 1 0 0 0
Vac/Vac Membrane – – 87 22 20 16 – 10 5 5 3 7 4 10 12 25 69 66 19 13 39 10 52 122
Unique Cell Count 323 241 369 480 631 430 645 381 522 62 178 161 63 158 142 71 46 394 85 233 265
Labelled Cell Count 426 334 496 611 881 574 800 476 682 75 207 181 81 200 197 71 46 394 85 233 265


Golgi

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) – – 6.7 7.7 7.7 7.2 – 7.3 7.1 6.6 6.7 7.1 6.8 6.5 6.2 5.6 7.0 8.2 7.4 7.6 7.5
Std Deviation (1e-4) – – 1.2 1.7 1.6 1.2 – 1.3 1.4 1.3 1.4 1.3 0.9 1.0 0.9 1.0 1.4 1.6 1.3 1.5 1.8
Intensity Change (Log2) – – – 0.2 0.21 0.1 – 0.13 0.09 -0.02 0.01 0.08 0.02 -0.04 -0.11 -0.25 0.07 0.29 0.14 0.18 0.17


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 3.3 0 – 0 0 0 0 0 0 6.9 5.2 0 0 0
Bud 2.4 2.7 1.8 – 2.9 4.9 3.2 4.2 3.7 0 0 0 0 0 0
Bud Neck 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm -0.5 -0.7 -4.3 – -5.1 -4.9 -7.2 -4.9 -6.0 -0.5 4.7 11.5 10.7 6.7 7.8
Endoplasmic Reticulum -1.8 -0.8 -2.9 – 0 -2.9 0 -3.4 -3.7 0 4.4 3.0 0 -0.3 -0.1
Endosome 2.9 2.6 -2.8 – -6.0 -11.5 -13.1 -11.6 -11.1 -3.0 -3.9 -6.3 -5.3 -3.2 -3.0
Golgi 1.6 2.1 -2.0 – -5.7 -6.0 -7.9 -6.5 -7.4 -1.1 -2.9 -4.3 -3.1 -2.8 -2.2
Mitochondria -0.2 -2.3 7.7 – 8.2 14.1 17.0 14.8 15.5 -2.7 -5.8 -7.0 -4.1 -4.8 -4.6
Nucleus 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 3.9 – 5.3 4.2 4.4 2.9 3.2 0 0 0 0 0 0
Peroxisomes 0 3.7 3.8 – 8.2 5.6 4.9 4.3 6.2 0 0 0 0 0 0
SpindlePole 2.1 2.5 1.3 – 4.7 2.3 1.4 2.2 1.6 0 4.4 -0.2 0 -0.8 0
Vacuole -5.3 -7.8 -9.8 – -12.3 -10.7 -13.1 -10.4 -11.5 -3.5 -5.8 -5.0 2.0 3.7 4.1

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 4.2135 5.5351 4.782 5.3625 4.5849 4.9952 4.3538 6.594 5.7137 4.9707 5.2354 5.8213 4.9152 4.207 4.0103 5.9059 5.5306 4.4653
Actin 0.0357 0.0083 0.0273 0.0006 0.0022 0.0045 0.0083 0.0027 0.0019 0.0008 0.0061 0.0111 0.0049 0.0028 0.0028 0.0038 0.0001 0.001
Bud 0.0006 0.0045 0.0051 0.0001 0.0001 0.0001 0.0002 0.0002 0.0001 0.0001 0.0005 0 0.0003 0.0001 0.0001 0.0002 0 0
Bud Neck 0.0035 0.0004 0.0007 0.0001 0.0004 0.0003 0.0002 0.0002 0.0001 0.0003 0.0003 0.0002 0.0004 0.0002 0.0002 0.0004 0.0001 0.0001
Bud Periphery 0.0009 0.0015 0.0023 0.0001 0.0001 0.0001 0.0002 0.0002 0.0001 0.0001 0.0008 0.0001 0.0002 0.0001 0.0001 0.0002 0 0
Bud Site 0.0057 0.0108 0.0033 0.0004 0.0002 0.0001 0.0003 0.0006 0.0001 0.0002 0.0011 0.0001 0.0006 0.0001 0.0003 0.0003 0 0
Cell Periphery 0.0003 0.0003 0.0001 0.0001 0 0.0001 0.0001 0 0 0.0001 0.0001 0 0.0001 0.0001 0.0001 0 0 0.0001
Cytoplasm 0.0147 0.0046 0.0017 0.0018 0.001 0.0033 0.0018 0.0011 0.0008 0.0008 0.0008 0.0006 0.0014 0.0005 0.0005 0.0004 0.0002 0.0003
Cytoplasmic Foci 0.089 0.0974 0.0966 0.178 0.1219 0.086 0.0761 0.06 0.0553 0.0596 0.0835 0.0498 0.065 0.0884 0.0567 0.068 0.023 0.0503
Eisosomes 0.0002 0 0.0002 0 0 0 0.0001 0 0 0 0.0001 0 0.0001 0 0.0001 0 0 0
Endoplasmic Reticulum 0.0046 0.0008 0.0006 0.0005 0.0617 0.0019 0.0014 0.0004 0.0004 0.0008 0.0004 0.0004 0.0011 0.0005 0.0004 0.0001 0.0004 0.0004
Endosome 0.2738 0.312 0.2594 0.292 0.1274 0.2575 0.253 0.2834 0.2188 0.31 0.2825 0.1761 0.4453 0.1955 0.2513 0.3086 0.3032 0.1942
Golgi 0.4558 0.496 0.5193 0.4755 0.588 0.5644 0.4974 0.6101 0.6784 0.5166 0.5407 0.6767 0.375 0.5868 0.5465 0.5359 0.6081 0.4877
Lipid Particles 0.0362 0.0081 0.0137 0.0203 0.0391 0.0174 0.0284 0.004 0.0036 0.0077 0.0096 0.0143 0.0436 0.099 0.0063 0.0045 0.0125 0.2144
Mitochondria 0.0254 0.0171 0.0386 0.0129 0.0366 0.0335 0.0386 0.0262 0.0293 0.0152 0.0547 0.0357 0.0346 0.0128 0.1162 0.0291 0.0061 0.0183
None 0.0025 0.0003 0.0004 0.0016 0.0001 0.0002 0.0117 0 0 0 0.0001 0 0.0002 0 0.0001 0.0001 0 0
Nuclear Periphery 0.0045 0.0004 0.0003 0.0004 0.0004 0.0077 0.0146 0.0001 0.0001 0.0005 0.0002 0.0001 0.0006 0.0002 0.0001 0 0.0006 0.0003
Nucleolus 0.0002 0.0004 0.0002 0.0011 0.0001 0.0001 0.0002 0 0 0.0001 0.0002 0 0.0001 0 0 0 0 0
Nucleus 0.0017 0.0002 0.0001 0.0004 0 0.003 0.0005 0 0 0.0002 0.0001 0 0.0002 0 0 0 0 0
Peroxisomes 0.0357 0.0182 0.0192 0.0079 0.0185 0.011 0.027 0.004 0.007 0.005 0.0122 0.0276 0.0088 0.0075 0.0127 0.0428 0.0003 0.0207
Punctate Nuclear 0.0022 0.0002 0.0009 0.0028 0.0009 0.0005 0.0071 0.0001 0 0.0001 0.0002 0.0001 0.0004 0.0002 0 0.0001 0 0.0002
Vacuole 0.0037 0.0081 0.0037 0.0013 0.0005 0.003 0.0127 0.0025 0.0012 0.0602 0.0023 0.0018 0.0085 0.0015 0.0016 0.0014 0.0113 0.004
Vacuole Periphery 0.0029 0.0105 0.0064 0.0021 0.0007 0.0052 0.0202 0.0042 0.0026 0.0217 0.0036 0.0052 0.0085 0.0037 0.0039 0.0038 0.0339 0.0078

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 84.3422 85.5015 72.6743 63.3533 58.6224 51.2725 80.9491 77.1788 65.2063 73.5477
Translational Efficiency 0.7321 0.7659 0.6851 0.6702 0.7225 0.8854 0.7492 0.6019 0.6265 0.6263

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Subunit of a Golgi mannosyltransferase complex; this complex also contains Anp1p, Mnn9p, Mnn10p, and Hoc1p, and mediates elongation of the polysaccharide mannan backbone; has homology to Mnn10p
Localization
Cell Percentages Golgi (49%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Mnn11

Mnn11


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Mnn11-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available