Standard name | |
---|---|
Human Ortholog | |
Description | Late endosomal protein involved in late endosome to vacuole transport; functional homolog of human obesity receptor gene-related protein (OB-RGRP) |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 64 | 36 | 56 | 23 | 49 | 50 | – | 39 | 29 | 20 | 25 | 19 | 40 | 33 | 29 | 25 | 15 | 17 | 7 | 5 | 5 | 2 | 3 | 3 |
Bud | 10 | 6 | 18 | 15 | 10 | 10 | – | 21 | 23 | 15 | 12 | 15 | 2 | 4 | 2 | 2 | 2 | 0 | 2 | 1 | 1 | 0 | 1 | 1 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 11 | 5 | 10 | 3 | 8 | 3 |
Bud Site | 1 | 0 | 0 | 1 | 2 | 3 | – | 1 | 0 | 1 | 0 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 341 | 214 | 162 | 159 | 161 | 229 | – | 141 | 208 | 145 | 146 | 181 | 169 | 144 | 186 | 166 | 131 | 146 | 427 | 254 | 248 | 81 | 170 | 158 |
Cytoplasm | 117 | 55 | 75 | 57 | 91 | 168 | – | 258 | 196 | 208 | 135 | 180 | 82 | 75 | 91 | 37 | 27 | 51 | 25 | 8 | 6 | 2 | 4 | 3 |
Endoplasmic Reticulum | 2 | 0 | 1 | 1 | 5 | 6 | – | 4 | 0 | 6 | 3 | 5 | 0 | 2 | 1 | 13 | 10 | 7 | 3 | 4 | 2 | 1 | 4 | 4 |
Endosome | 2 | 0 | 0 | 0 | 0 | 0 | – | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 8 | 1 | 2 | 0 | 0 | 1 |
Golgi | 0 | 0 | 0 | 0 | 1 | 0 | – | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 4 | 2 | 2 | 0 | 0 | 0 | 1 |
Mitochondria | 134 | 48 | 102 | 175 | 136 | 252 | – | 278 | 308 | 233 | 256 | 337 | 39 | 49 | 40 | 19 | 16 | 22 | 7 | 5 | 6 | 0 | 1 | 3 |
Nucleus | 2 | 2 | 0 | 0 | 1 | 0 | – | 4 | 0 | 2 | 2 | 4 | 0 | 1 | 0 | 3 | 4 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | – | 4 | 0 | 0 | 1 | 3 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 1 | – | 0 | 2 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 1 | 0 | 0 | 0 | 2 | 4 | – | 1 | 2 | 2 | 0 | 0 | 7 | 15 | 21 | 2 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 0 |
SpindlePole | 6 | 3 | 2 | 1 | 4 | 2 | – | 0 | 1 | 2 | 0 | 1 | 2 | 2 | 2 | 1 | 1 | 2 | 0 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 10 | 0 | 5 | 4 | 3 | 1 | – | 11 | 8 | 2 | 6 | 9 | 0 | 2 | 1 | 7 | 1 | 4 | 10 | 4 | 2 | 1 | 3 | 0 |
Unique Cell Count | 453 | 260 | 254 | 248 | 290 | 444 | 530 | 484 | 418 | 385 | 484 | 217 | 205 | 239 | 202 | 157 | 192 | 513 | 297 | 291 | 100 | 203 | 187 | |
Labelled Cell Count | 690 | 364 | 421 | 436 | 465 | 726 | 764 | 777 | 636 | 586 | 755 | 344 | 328 | 377 | 275 | 209 | 254 | 513 | 297 | 291 | 100 | 203 | 187 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 6.9 | 7.6 | 5.1 | 5.6 | 6.2 | 5.2 | – | 5.2 | 4.8 | 5.0 | 4.7 | 4.7 | 7.3 | 7.6 | 7.5 | 9.6 | 9.5 | 9.2 | 7.2 | 8.0 | 8.0 |
Std Deviation (1e-4) | 0.7 | 1.0 | 1.2 | 1.3 | 1.5 | 1.0 | – | 1.0 | 1.1 | 0.9 | 1.0 | 1.0 | 1.5 | 1.7 | 1.7 | 1.7 | 1.7 | 2.1 | 1.3 | 1.8 | 1.8 |
Intensity Change (Log2) | – | – | – | 0.12 | 0.29 | 0.02 | – | 0.02 | -0.09 | -0.05 | -0.11 | -0.13 | 0.51 | 0.57 | 0.56 | 0.9 | 0.9 | 0.85 | 0.49 | 0.65 | 0.65 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | -3.9 | -1.5 | -3.8 | – | -5.9 | 0 | -6.9 | 0 | 0 | -1.0 | -1.6 | -2.9 | -2.7 | -3.3 | -3.7 |
Bud | -0.5 | -1.9 | -3.1 | – | -1.9 | 0 | -2.0 | 0 | 0 | -3.3 | -2.6 | -3.5 | -3.2 | -2.7 | -3.8 |
Bud Neck | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0.1 | -2.0 | -3.1 | – | -10.0 | 0 | -7.3 | 0 | 0 | 3.3 | 1.5 | 3.4 | 4.3 | 4.3 | 2.8 |
Cytoplasm | -1.7 | 0.5 | 2.2 | – | 5.1 | 2.9 | 5.2 | 1.5 | 2.1 | 1.9 | 1.6 | 2.0 | -2.8 | -2.8 | -0.7 |
Endoplasmic Reticulum | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3.7 | 0 | 0 |
Endosome | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 6.8 | 1.6 | 4.2 | – | 3.2 | 0 | 3.9 | 0 | 0 | -5.2 | -3.7 | -5.7 | -7.4 | -6.5 | -6.7 |
Nucleus | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 4.4 | 4.8 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | – | 0.1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1.0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 1.7252 | 3.0299 | 2.4017 | 2.8676 | 1.5294 | 2.839 | 2.5367 | 2.5002 | 2.3628 | 2.0076 | 2.6321 | 2.8081 | 3.5985 | 3.8848 | 3.6899 | 4.6547 | 3.2532 | 3.8413 |
Actin | 0.0101 | 0.0001 | 0.0144 | 0.0225 | 0 | 0.0152 | 0.0126 | 0.0005 | 0.0038 | 0.0088 | 0.0008 | 0.0016 | 0.0123 | 0.0001 | 0.0039 | 0.0164 | 0.0001 | 0.0001 |
Bud | 0.0001 | 0.0001 | 0.0003 | 0.0004 | 0 | 0.0001 | 0.0006 | 0.0001 | 0.0007 | 0.0001 | 0.0001 | 0.0004 | 0.0002 | 0.0002 | 0.0003 | 0.0007 | 0 | 0 |
Bud Neck | 0.0004 | 0.0011 | 0.0008 | 0.0012 | 0.0001 | 0.0029 | 0.0012 | 0.0011 | 0.0005 | 0.0002 | 0.0002 | 0.0006 | 0.0004 | 0.0004 | 0.0004 | 0.0002 | 0.0001 | 0.0004 |
Bud Periphery | 0.0003 | 0.0001 | 0.0003 | 0.0004 | 0 | 0.0002 | 0.001 | 0.0001 | 0.0006 | 0.0001 | 0.0001 | 0.0003 | 0.0002 | 0.0003 | 0.0003 | 0.001 | 0 | 0 |
Bud Site | 0.0008 | 0.0011 | 0.0024 | 0.0043 | 0 | 0.0009 | 0.0028 | 0.0011 | 0.0024 | 0.0004 | 0.0002 | 0.0006 | 0.0013 | 0.0021 | 0.001 | 0.0009 | 0.0001 | 0.0001 |
Cell Periphery | 0.0003 | 0.0002 | 0.0001 | 0.0001 | 0 | 0.0001 | 0.0011 | 0.0001 | 0.0001 | 0 | 0 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0 | 0 |
Cytoplasm | 0.0034 | 0.0113 | 0.0098 | 0.0009 | 0.0003 | 0.0388 | 0.0082 | 0.0047 | 0.0059 | 0.024 | 0.0013 | 0.0057 | 0.0015 | 0.0141 | 0.0034 | 0.0017 | 0.0006 | 0.016 |
Cytoplasmic Foci | 0.1285 | 0.1431 | 0.1075 | 0.0809 | 0.0932 | 0.0918 | 0.1418 | 0.2032 | 0.1841 | 0.1283 | 0.1245 | 0.1743 | 0.1562 | 0.1971 | 0.2062 | 0.1195 | 0.1543 | 0.1702 |
Eisosomes | 0.0007 | 0 | 0.0001 | 0.0002 | 0 | 0.0001 | 0.0008 | 0 | 0 | 0 | 0 | 0 | 0.0001 | 0 | 0.0001 | 0.0001 | 0 | 0 |
Endoplasmic Reticulum | 0.001 | 0.0008 | 0.0032 | 0.0005 | 0.0002 | 0.0008 | 0.0056 | 0.0005 | 0.0015 | 0.0005 | 0.0012 | 0.0013 | 0.0009 | 0.0021 | 0.0006 | 0.0002 | 0.0002 | 0.0013 |
Endosome | 0.4842 | 0.5229 | 0.5334 | 0.5819 | 0.6265 | 0.3877 | 0.5484 | 0.5387 | 0.5147 | 0.6347 | 0.5768 | 0.4518 | 0.4458 | 0.486 | 0.4982 | 0.6671 | 0.5696 | 0.4568 |
Golgi | 0.1171 | 0.1125 | 0.1484 | 0.2005 | 0.0907 | 0.1099 | 0.0711 | 0.0712 | 0.1284 | 0.0804 | 0.0921 | 0.1094 | 0.0919 | 0.0874 | 0.1206 | 0.0709 | 0.106 | 0.0983 |
Lipid Particles | 0.1602 | 0.1236 | 0.0889 | 0.027 | 0.1366 | 0.2961 | 0.0772 | 0.0765 | 0.064 | 0.0537 | 0.016 | 0.1497 | 0.1664 | 0.0992 | 0.0693 | 0.0669 | 0.1462 | 0.1662 |
Mitochondria | 0.0123 | 0.0023 | 0.0074 | 0.0307 | 0.0013 | 0.0048 | 0.0215 | 0.0089 | 0.0061 | 0.0068 | 0.1211 | 0.0111 | 0.0219 | 0.002 | 0.0221 | 0.0116 | 0.0012 | 0.0016 |
None | 0.0061 | 0.0012 | 0.0053 | 0.0001 | 0 | 0.0007 | 0.0107 | 0.0006 | 0.0025 | 0.001 | 0.0001 | 0.0026 | 0.0007 | 0.0151 | 0.0014 | 0.0002 | 0 | 0.0026 |
Nuclear Periphery | 0.0015 | 0.0007 | 0.0031 | 0.0004 | 0.0002 | 0.0014 | 0.0111 | 0.0007 | 0.0015 | 0.0003 | 0.0008 | 0.0057 | 0.0024 | 0.0022 | 0.0003 | 0.0003 | 0.0002 | 0.0004 |
Nucleolus | 0.0014 | 0.0064 | 0.0008 | 0.0001 | 0.0001 | 0.0003 | 0.0032 | 0.0007 | 0.0006 | 0.0003 | 0.0001 | 0.0106 | 0.0003 | 0.0029 | 0.0003 | 0.0001 | 0.0005 | 0.0004 |
Nucleus | 0.0008 | 0.0011 | 0.0011 | 0.0001 | 0 | 0.0005 | 0.0021 | 0.0005 | 0.0003 | 0.0002 | 0.0001 | 0.003 | 0.0007 | 0.0006 | 0.0003 | 0.0001 | 0.0001 | 0.0001 |
Peroxisomes | 0.021 | 0.0141 | 0.0262 | 0.0245 | 0.0222 | 0.0168 | 0.0346 | 0.0468 | 0.0638 | 0.0244 | 0.0566 | 0.0479 | 0.0717 | 0.0507 | 0.0497 | 0.0254 | 0.0144 | 0.0693 |
Punctate Nuclear | 0.01 | 0.0008 | 0.0033 | 0.0003 | 0.0003 | 0.0013 | 0.0082 | 0.0059 | 0.0025 | 0.001 | 0.0003 | 0.0035 | 0.0037 | 0.0052 | 0.0012 | 0.0006 | 0.0006 | 0.0018 |
Vacuole | 0.0296 | 0.0424 | 0.0283 | 0.0104 | 0.0179 | 0.011 | 0.0266 | 0.0311 | 0.011 | 0.0177 | 0.0044 | 0.0114 | 0.0156 | 0.025 | 0.0168 | 0.0104 | 0.0031 | 0.0103 |
Vacuole Periphery | 0.0102 | 0.0141 | 0.0148 | 0.0126 | 0.0102 | 0.0185 | 0.0097 | 0.007 | 0.005 | 0.0171 | 0.003 | 0.0082 | 0.0059 | 0.007 | 0.0034 | 0.0057 | 0.0028 | 0.0039 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 36.5193 | 34.9779 | 29.4158 | 63.5096 | 27.0565 | 31.2849 | 45.3865 | 65.2439 | 45.686 | 52.1444 |
Translational Efficiency | 1.8864 | 1.3723 | 1.8433 | 0.6249 | 1.7794 | 3.1288 | 1.4913 | 1.0782 | 1.6771 | 1.471 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✔ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
349 | 1194 | 2435 | 860 | 840 | 1775 | 140 | 256 | 1189 | 2969 | 2575 | 1116 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 602.02 | 844.47 | 1293.97 | 976.45 | 727.82 | 847.74 | 1167.55 | 1137.79 | 690.89 | 846.42 | 1287.10 | 1013.46 |
Standard Deviation | 107.55 | 122.22 | 216.06 | 177.42 | 124.65 | 127.77 | 162.32 | 211.49 | 132.87 | 125.58 | 215.40 | 197.78 |
Intensity Change Log 2 | — | 0.488235 | 1.103921 | 0.697735 | — | 0.220040 | 0.681831 | 0.644581 | — | 0.347656 | 0.888297 | 0.668886 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000413 | 0.000357 | 0.000254 | 0.001375 | 0.000425 | 0.000472 | 0.000368 | 0.001276 | 0.000421 | 0.000426 | 0.000260 | 0.001352 |
Bud Neck | 0.041212 | 0.003320 | 0.003178 | 0.005785 | 0.011285 | 0.005331 | 0.004005 | 0.003290 | 0.020069 | 0.004522 | 0.003223 | 0.005212 |
Bud Site | 0.018784 | 0.008693 | 0.004032 | 0.019055 | 0.022973 | 0.008113 | 0.008391 | 0.034002 | 0.021743 | 0.008346 | 0.004269 | 0.022484 |
Cell Periphery | 0.000237 | 0.000076 | 0.000166 | 0.000085 | 0.000314 | 0.000099 | 0.000072 | 0.000131 | 0.000291 | 0.000090 | 0.000161 | 0.000096 |
Cytoplasm | 0.096495 | 0.012697 | 0.000951 | 0.017939 | 0.020294 | 0.012259 | 0.001713 | 0.019009 | 0.042661 | 0.012435 | 0.000992 | 0.018184 |
Cytoplasmic Foci | 0.192511* | 0.142640 | 0.031939 | 0.127093 | 0.134590 | 0.123848 | 0.154639 | 0.149334 | 0.151591 | 0.131406 | 0.038610 | 0.132195 |
Eisosomes | 0.000165 | 0.000031 | 0.000019 | 0.000023 | 0.000063 | 0.000032 | 0.000020 | 0.000023 | 0.000093 | 0.000032 | 0.000019 | 0.000023 |
Endoplasmic Reticulum | 0.000856 | 0.000656 | 0.000420 | 0.000729 | 0.000845 | 0.000832 | 0.000309 | 0.000316 | 0.000848 | 0.000761 | 0.000414 | 0.000634 |
Endosome | 0.402149* | 0.521884* | 0.152272 | 0.391614* | 0.445005* | 0.487276* | 0.560997* | 0.352751* | 0.432426* | 0.501194* | 0.174494 | 0.382699* |
Golgi | 0.032507 | 0.110156* | 0.094481 | 0.093653 | 0.089151 | 0.105322 | 0.067401 | 0.196569* | 0.072524 | 0.107266 | 0.093009 | 0.117261* |
Lipid Particles | 0.028776 | 0.006831 | 0.006998 | 0.006417 | 0.003018 | 0.005619 | 0.008081 | 0.001241 | 0.010578 | 0.006106 | 0.007056 | 0.005230 |
Mitochondria | 0.010603 | 0.025975 | 0.035999 | 0.007275 | 0.025816 | 0.034843 | 0.003516 | 0.012237 | 0.021351 | 0.031277 | 0.034233 | 0.008413 |
Mitotic Spindle | 0.002013 | 0.013128 | 0.013905 | 0.054308 | 0.005002 | 0.008211 | 0.014087 | 0.024545 | 0.004124 | 0.010189 | 0.013915 | 0.047481 |
None | 0.009347 | 0.000273 | 0.000263 | 0.000242 | 0.000228 | 0.000303 | 0.000013 | 0.000070 | 0.002905 | 0.000291 | 0.000249 | 0.000203 |
Nuclear Periphery | 0.000617 | 0.000433 | 0.007602 | 0.002011 | 0.000199 | 0.000437 | 0.000452 | 0.000154 | 0.000322 | 0.000435 | 0.007213 | 0.001585 |
Nuclear Periphery Foci | 0.001740 | 0.001274 | 0.013275 | 0.008051 | 0.000967 | 0.001645 | 0.004433 | 0.000811 | 0.001194 | 0.001496 | 0.012795 | 0.006390 |
Nucleolus | 0.008159 | 0.001629 | 0.002996 | 0.001429 | 0.002031 | 0.000854 | 0.000475 | 0.000151 | 0.003830 | 0.001166 | 0.002859 | 0.001136 |
Nucleus | 0.025518 | 0.001343 | 0.001212 | 0.001524 | 0.004150 | 0.002163 | 0.001425 | 0.000278 | 0.010422 | 0.001833 | 0.001223 | 0.001238 |
Peroxisomes | 0.038174 | 0.009957 | 0.001650 | 0.011048 | 0.011079 | 0.010212 | 0.025879 | 0.017206 | 0.019032 | 0.010110 | 0.002968 | 0.012460 |
Vacuole | 0.080222 | 0.096160 | 0.334841* | 0.215240* | 0.140332 | 0.122036 | 0.120590 | 0.153765 | 0.122688 | 0.111630 | 0.323193* | 0.201138* |
Vacuole Periphery | 0.009502 | 0.042488 | 0.293547* | 0.035103 | 0.082234 | 0.070092 | 0.023136 | 0.032838 | 0.060885 | 0.058991 | 0.278845* | 0.034584 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | 0.21 | 0.66 | -1.66 | -1.82 | -2.07 | -0.16 | 0.31 | -2.19 | -1.94 | -2.44 | 0.00 | 1.08 | -2.12 | -2.09 | -2.56 |
Bud Neck | 6.71 | 6.73 | 6.05 | -3.63 | -3.85 | 3.51 | 3.75 | 4.71 | 2.04 | 0.74 | 7.52 | 8.20 | 6.61 | -1.81 | -3.63 |
Bud Site | 2.92 | 4.45 | -0.61 | -5.55 | -8.57 | 6.30 | 4.19 | -1.86 | -4.56 | -4.08 | 6.86 | 9.22 | -0.88 | -7.53 | -9.72 |
Cell Periphery | 5.28 | 2.34 | 4.73 | -0.96 | 6.26 | 3.43 | 3.83 | 2.74 | -0.96 | -1.76 | 4.48 | 2.90 | 4.24 | -0.65 | 5.25 |
Cytoplasm | 8.18 | 9.44 | 6.75 | -3.90 | -8.49 | 3.16 | 8.59 | -0.33 | -2.15 | -4.78 | 8.34 | 11.99 | 4.26 | -4.62 | -9.76 |
Cytoplasmic Foci | 4.85 | 16.57 | 6.68 | 3.28 | -19.66 | 1.71 | -1.34 | -1.21 | -2.39 | 0.44 | 3.81 | 23.00 | 3.82 | 0.66 | -20.88 |
Eisosomes | 5.96 | 6.54 | 6.29 | 1.77 | -1.91 | 3.28 | 4.58 | 4.31 | 1.72 | -0.41 | 6.53 | 8.12 | 7.48 | 2.34 | -2.09 |
Endoplasmic Reticulum | 0.89 | 2.03 | 0.29 | -0.93 | -3.01 | 0.12 | 4.17 | 4.37 | 3.10 | -0.11 | 0.68 | 4.37 | 1.32 | 0.63 | -2.78 |
Endosome | -6.80 | 14.79 | 1.50 | 12.56 | -22.32 | -3.63 | -4.65 | 5.49 | 8.43 | 7.89 | -7.15 | 27.49 | 5.82 | 14.60 | -21.42 |
Golgi | -14.61 | -14.06 | -9.15 | 3.52 | 1.24 | -2.89 | 2.39 | -6.58 | -5.60 | -7.29 | -8.15 | -4.62 | -6.10 | -0.56 | -2.96 |
Lipid Particles | 4.37 | 4.43 | 4.36 | 0.09 | 0.19 | -2.83 | -1.61 | 2.66 | 5.08 | 2.14 | 2.70 | 2.17 | 2.75 | 0.52 | 1.24 |
Mitochondria | -5.69 | -10.34 | 1.94 | 7.33 | 12.42 | -2.81 | 6.85 | 3.30 | 5.76 | -2.07 | -4.19 | -4.89 | 6.07 | 11.86 | 11.58 |
Mitotic Spindle | -5.26 | -10.01 | -10.81 | -8.15 | -8.34 | -2.24 | -2.03 | -3.82 | -3.20 | -1.71 | -4.72 | -7.55 | -10.95 | -9.40 | -8.49 |
None | 2.85 | 2.86 | 2.85 | -0.01 | -0.19 | -0.67 | 2.44 | 1.71 | 3.08 | -2.01 | 2.78 | 2.82 | 2.84 | 0.82 | 0.31 |
Nuclear Periphery | 1.07 | -11.93 | -3.04 | -3.55 | 7.78 | -2.07 | -1.65 | 0.18 | 3.47 | 2.13 | -1.17 | -12.70 | -3.67 | -3.40 | 8.83 |
Nuclear Periphery Foci | 1.19 | -11.15 | -3.89 | -4.23 | 2.59 | -1.07 | -1.56 | 0.38 | 1.91 | 1.68 | -0.57 | -11.67 | -4.03 | -3.93 | 3.92 |
Nucleolus | 2.49 | 1.98 | 2.46 | -0.23 | 2.28 | 1.43 | 1.93 | 2.49 | 2.80 | 2.04 | 2.75 | 1.14 | 2.61 | -0.32 | 3.12 |
Nucleus | 4.78 | 4.80 | 4.64 | -1.64 | -2.21 | 1.20 | 1.43 | 2.30 | 2.18 | 0.84 | 4.45 | 4.86 | 4.61 | 0.28 | -1.45 |
Peroxisomes | 5.68 | 7.57 | 5.45 | -0.67 | -7.74 | 0.62 | -3.15 | -1.46 | -1.76 | 1.65 | 4.84 | 9.17 | 3.13 | -1.60 | -7.22 |
Vacuole | -1.75 | -28.24 | -12.95 | -14.28 | 11.29 | 2.35 | 0.87 | -1.83 | -3.30 | -2.04 | 1.79 | -30.32 | -10.47 | -13.56 | 13.14 |
Vacuole Periphery | -11.79 | -58.76* | -9.01 | 2.84 | 50.54* | 2.12 | 8.17 | 7.71 | 7.43 | -1.33 | 0.45 | -37.28 | 6.74 | 9.27 | 49.48* |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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