Standard name | |
---|---|
Human Ortholog | |
Description | Late endosomal protein involved in late endosome to vacuole transport; functional homolog of human obesity receptor gene-related protein (OB-RGRP) |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 64 | 36 | 56 | 23 | 49 | 50 | – | 39 | 29 | 20 | 25 | 19 | 40 | 33 | 29 | 25 | 15 | 17 | 7 | 5 | 5 | 2 | 3 | 3 |
Bud | 10 | 6 | 18 | 15 | 10 | 10 | – | 21 | 23 | 15 | 12 | 15 | 2 | 4 | 2 | 2 | 2 | 0 | 2 | 1 | 1 | 0 | 1 | 1 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 11 | 5 | 10 | 3 | 8 | 3 |
Bud Site | 1 | 0 | 0 | 1 | 2 | 3 | – | 1 | 0 | 1 | 0 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 341 | 214 | 162 | 159 | 161 | 229 | – | 141 | 208 | 145 | 146 | 181 | 169 | 144 | 186 | 166 | 131 | 146 | 427 | 254 | 248 | 81 | 170 | 158 |
Cytoplasm | 117 | 55 | 75 | 57 | 91 | 168 | – | 258 | 196 | 208 | 135 | 180 | 82 | 75 | 91 | 37 | 27 | 51 | 25 | 8 | 6 | 2 | 4 | 3 |
Endoplasmic Reticulum | 2 | 0 | 1 | 1 | 5 | 6 | – | 4 | 0 | 6 | 3 | 5 | 0 | 2 | 1 | 13 | 10 | 7 | 3 | 4 | 2 | 1 | 4 | 4 |
Endosome | 2 | 0 | 0 | 0 | 0 | 0 | – | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 8 | 1 | 2 | 0 | 0 | 1 |
Golgi | 0 | 0 | 0 | 0 | 1 | 0 | – | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 4 | 2 | 2 | 0 | 0 | 0 | 1 |
Mitochondria | 134 | 48 | 102 | 175 | 136 | 252 | – | 278 | 308 | 233 | 256 | 337 | 39 | 49 | 40 | 19 | 16 | 22 | 7 | 5 | 6 | 0 | 1 | 3 |
Nucleus | 2 | 2 | 0 | 0 | 1 | 0 | – | 4 | 0 | 2 | 2 | 4 | 0 | 1 | 0 | 3 | 4 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | – | 4 | 0 | 0 | 1 | 3 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 1 | – | 0 | 2 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 1 | 0 | 0 | 0 | 2 | 4 | – | 1 | 2 | 2 | 0 | 0 | 7 | 15 | 21 | 2 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 0 |
SpindlePole | 6 | 3 | 2 | 1 | 4 | 2 | – | 0 | 1 | 2 | 0 | 1 | 2 | 2 | 2 | 1 | 1 | 2 | 0 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 10 | 0 | 5 | 4 | 3 | 1 | – | 11 | 8 | 2 | 6 | 9 | 0 | 2 | 1 | 7 | 1 | 4 | 10 | 4 | 2 | 1 | 3 | 0 |
Unique Cell Count | 453 | 260 | 254 | 248 | 290 | 444 | 530 | 484 | 418 | 385 | 484 | 217 | 205 | 239 | 202 | 157 | 192 | 513 | 297 | 291 | 100 | 203 | 187 | |
Labelled Cell Count | 690 | 364 | 421 | 436 | 465 | 726 | 764 | 777 | 636 | 586 | 755 | 344 | 328 | 377 | 275 | 209 | 254 | 513 | 297 | 291 | 100 | 203 | 187 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 6.9 | 7.6 | 5.1 | 5.6 | 6.2 | 5.2 | – | 5.2 | 4.8 | 5.0 | 4.7 | 4.7 | 7.3 | 7.6 | 7.5 | 9.6 | 9.5 | 9.2 | 7.2 | 8.0 | 8.0 |
Std Deviation (1e-4) | 0.7 | 1.0 | 1.2 | 1.3 | 1.5 | 1.0 | – | 1.0 | 1.1 | 0.9 | 1.0 | 1.0 | 1.5 | 1.7 | 1.7 | 1.7 | 1.7 | 2.1 | 1.3 | 1.8 | 1.8 |
Intensity Change (Log2) | – | – | – | 0.12 | 0.29 | 0.02 | – | 0.02 | -0.09 | -0.05 | -0.11 | -0.13 | 0.51 | 0.57 | 0.56 | 0.9 | 0.9 | 0.85 | 0.49 | 0.65 | 0.65 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | -3.9 | -1.5 | -3.8 | – | -5.9 | 0 | -6.9 | 0 | 0 | -1.0 | -1.6 | -2.9 | -2.7 | -3.3 | -3.7 |
Bud | -0.5 | -1.9 | -3.1 | – | -1.9 | 0 | -2.0 | 0 | 0 | -3.3 | -2.6 | -3.5 | -3.2 | -2.7 | -3.8 |
Bud Neck | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0.1 | -2.0 | -3.1 | – | -10.0 | 0 | -7.3 | 0 | 0 | 3.3 | 1.5 | 3.4 | 4.3 | 4.3 | 2.8 |
Cytoplasm | -1.7 | 0.5 | 2.2 | – | 5.1 | 2.9 | 5.2 | 1.5 | 2.1 | 1.9 | 1.6 | 2.0 | -2.8 | -2.8 | -0.7 |
Endoplasmic Reticulum | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3.7 | 0 | 0 |
Endosome | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 6.8 | 1.6 | 4.2 | – | 3.2 | 0 | 3.9 | 0 | 0 | -5.2 | -3.7 | -5.7 | -7.4 | -6.5 | -6.7 |
Nucleus | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 4.4 | 4.8 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | – | 0.1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1.0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 1.7252 | 3.0299 | 2.4017 | 2.8676 | 1.5294 | 2.839 | 2.5367 | 2.5002 | 2.3628 | 2.0076 | 2.6321 | 2.8081 | 3.5985 | 3.8848 | 3.6899 | 4.6547 | 3.2532 | 3.8413 |
Actin | 0.0101 | 0.0001 | 0.0144 | 0.0225 | 0 | 0.0152 | 0.0126 | 0.0005 | 0.0038 | 0.0088 | 0.0008 | 0.0016 | 0.0123 | 0.0001 | 0.0039 | 0.0164 | 0.0001 | 0.0001 |
Bud | 0.0001 | 0.0001 | 0.0003 | 0.0004 | 0 | 0.0001 | 0.0006 | 0.0001 | 0.0007 | 0.0001 | 0.0001 | 0.0004 | 0.0002 | 0.0002 | 0.0003 | 0.0007 | 0 | 0 |
Bud Neck | 0.0004 | 0.0011 | 0.0008 | 0.0012 | 0.0001 | 0.0029 | 0.0012 | 0.0011 | 0.0005 | 0.0002 | 0.0002 | 0.0006 | 0.0004 | 0.0004 | 0.0004 | 0.0002 | 0.0001 | 0.0004 |
Bud Periphery | 0.0003 | 0.0001 | 0.0003 | 0.0004 | 0 | 0.0002 | 0.001 | 0.0001 | 0.0006 | 0.0001 | 0.0001 | 0.0003 | 0.0002 | 0.0003 | 0.0003 | 0.001 | 0 | 0 |
Bud Site | 0.0008 | 0.0011 | 0.0024 | 0.0043 | 0 | 0.0009 | 0.0028 | 0.0011 | 0.0024 | 0.0004 | 0.0002 | 0.0006 | 0.0013 | 0.0021 | 0.001 | 0.0009 | 0.0001 | 0.0001 |
Cell Periphery | 0.0003 | 0.0002 | 0.0001 | 0.0001 | 0 | 0.0001 | 0.0011 | 0.0001 | 0.0001 | 0 | 0 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0 | 0 |
Cytoplasm | 0.0034 | 0.0113 | 0.0098 | 0.0009 | 0.0003 | 0.0388 | 0.0082 | 0.0047 | 0.0059 | 0.024 | 0.0013 | 0.0057 | 0.0015 | 0.0141 | 0.0034 | 0.0017 | 0.0006 | 0.016 |
Cytoplasmic Foci | 0.1285 | 0.1431 | 0.1075 | 0.0809 | 0.0932 | 0.0918 | 0.1418 | 0.2032 | 0.1841 | 0.1283 | 0.1245 | 0.1743 | 0.1562 | 0.1971 | 0.2062 | 0.1195 | 0.1543 | 0.1702 |
Eisosomes | 0.0007 | 0 | 0.0001 | 0.0002 | 0 | 0.0001 | 0.0008 | 0 | 0 | 0 | 0 | 0 | 0.0001 | 0 | 0.0001 | 0.0001 | 0 | 0 |
Endoplasmic Reticulum | 0.001 | 0.0008 | 0.0032 | 0.0005 | 0.0002 | 0.0008 | 0.0056 | 0.0005 | 0.0015 | 0.0005 | 0.0012 | 0.0013 | 0.0009 | 0.0021 | 0.0006 | 0.0002 | 0.0002 | 0.0013 |
Endosome | 0.4842 | 0.5229 | 0.5334 | 0.5819 | 0.6265 | 0.3877 | 0.5484 | 0.5387 | 0.5147 | 0.6347 | 0.5768 | 0.4518 | 0.4458 | 0.486 | 0.4982 | 0.6671 | 0.5696 | 0.4568 |
Golgi | 0.1171 | 0.1125 | 0.1484 | 0.2005 | 0.0907 | 0.1099 | 0.0711 | 0.0712 | 0.1284 | 0.0804 | 0.0921 | 0.1094 | 0.0919 | 0.0874 | 0.1206 | 0.0709 | 0.106 | 0.0983 |
Lipid Particles | 0.1602 | 0.1236 | 0.0889 | 0.027 | 0.1366 | 0.2961 | 0.0772 | 0.0765 | 0.064 | 0.0537 | 0.016 | 0.1497 | 0.1664 | 0.0992 | 0.0693 | 0.0669 | 0.1462 | 0.1662 |
Mitochondria | 0.0123 | 0.0023 | 0.0074 | 0.0307 | 0.0013 | 0.0048 | 0.0215 | 0.0089 | 0.0061 | 0.0068 | 0.1211 | 0.0111 | 0.0219 | 0.002 | 0.0221 | 0.0116 | 0.0012 | 0.0016 |
None | 0.0061 | 0.0012 | 0.0053 | 0.0001 | 0 | 0.0007 | 0.0107 | 0.0006 | 0.0025 | 0.001 | 0.0001 | 0.0026 | 0.0007 | 0.0151 | 0.0014 | 0.0002 | 0 | 0.0026 |
Nuclear Periphery | 0.0015 | 0.0007 | 0.0031 | 0.0004 | 0.0002 | 0.0014 | 0.0111 | 0.0007 | 0.0015 | 0.0003 | 0.0008 | 0.0057 | 0.0024 | 0.0022 | 0.0003 | 0.0003 | 0.0002 | 0.0004 |
Nucleolus | 0.0014 | 0.0064 | 0.0008 | 0.0001 | 0.0001 | 0.0003 | 0.0032 | 0.0007 | 0.0006 | 0.0003 | 0.0001 | 0.0106 | 0.0003 | 0.0029 | 0.0003 | 0.0001 | 0.0005 | 0.0004 |
Nucleus | 0.0008 | 0.0011 | 0.0011 | 0.0001 | 0 | 0.0005 | 0.0021 | 0.0005 | 0.0003 | 0.0002 | 0.0001 | 0.003 | 0.0007 | 0.0006 | 0.0003 | 0.0001 | 0.0001 | 0.0001 |
Peroxisomes | 0.021 | 0.0141 | 0.0262 | 0.0245 | 0.0222 | 0.0168 | 0.0346 | 0.0468 | 0.0638 | 0.0244 | 0.0566 | 0.0479 | 0.0717 | 0.0507 | 0.0497 | 0.0254 | 0.0144 | 0.0693 |
Punctate Nuclear | 0.01 | 0.0008 | 0.0033 | 0.0003 | 0.0003 | 0.0013 | 0.0082 | 0.0059 | 0.0025 | 0.001 | 0.0003 | 0.0035 | 0.0037 | 0.0052 | 0.0012 | 0.0006 | 0.0006 | 0.0018 |
Vacuole | 0.0296 | 0.0424 | 0.0283 | 0.0104 | 0.0179 | 0.011 | 0.0266 | 0.0311 | 0.011 | 0.0177 | 0.0044 | 0.0114 | 0.0156 | 0.025 | 0.0168 | 0.0104 | 0.0031 | 0.0103 |
Vacuole Periphery | 0.0102 | 0.0141 | 0.0148 | 0.0126 | 0.0102 | 0.0185 | 0.0097 | 0.007 | 0.005 | 0.0171 | 0.003 | 0.0082 | 0.0059 | 0.007 | 0.0034 | 0.0057 | 0.0028 | 0.0039 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 36.5193 | 34.9779 | 29.4158 | 63.5096 | 27.0565 | 31.2849 | 45.3865 | 65.2439 | 45.686 | 52.1444 |
Translational Efficiency | 1.8864 | 1.3723 | 1.8433 | 0.6249 | 1.7794 | 3.1288 | 1.4913 | 1.0782 | 1.6771 | 1.471 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✔ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
---|
Gene | Images |
---|