Standard name
Human Ortholog
Description Member of the NineTeen Complex (NTC); NTC contains Prp19p and stabilizes U6 snRNA in catalytic forms of spliceosome containing U2, U5, and U6 snRNAs; interacts with Prp16p to modulate splicing fidelity; isy1 syf2 cells have defective spindles
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0.05 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0.08 0.08 0.1 0.09 – 0.07 0.1 0.05 0.06 0.07 0.16 0.17 0.16 0.08 0.06 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.1 0.05 0 0 0.05 0.42 0.1 – 0.43 0.32 0.42 0.32 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0.89 0.94 0.96 0.92 0.89 0.78 0.84 – 0.79 0.76 0.8 0.8 0.92 0.82 0.77 0.77 0.82 0.89 0.82 0.93 0.89 0.78 0.77 0.62
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.07 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.06 0.07
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0.08 0.06 0.06 0.06 0 0.06 0.09 0.1 0.18
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 5 0 – 2 8 5 5 0 0 0 1 0 1 1 0 1 1 3 5
Bud Neck 0 0 0 0 0 0 1 – 1 2 0 1 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 1 0 0 0 0 11 1 – 6 11 17 26 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 1 – 0 0 1 0 0 1 0 0 1 0 0 0 0 0 0 0
Cytoplasm 8 5 11 13 14 26 32 – 21 31 20 30 23 56 66 22 16 12 1 0 2 0 1 2
Endoplasmic Reticulum 0 0 1 0 0 1 0 – 0 0 0 0 0 1 1 3 2 1 0 0 0 0 1 2
Endosome 0 0 0 0 0 0 2 – 0 1 0 3 0 2 4 2 5 0 5 0 0 3 3 3
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0 1 1 0 1 1 0 0 0 0 0 1
Mitochondria 19 12 2 5 8 106 36 – 125 99 166 169 2 0 0 3 6 6 2 0 3 0 4 6
Nucleus 170 237 272 142 149 199 306 – 231 240 316 418 284 284 293 107 162 184 169 232 260 152 232 149
Nuclear Periphery 1 0 0 3 1 1 0 – 4 2 3 6 0 2 1 1 5 1 0 0 1 1 0 1
Nucleolus 14 2 0 0 0 0 1 – 8 8 10 8 0 0 2 1 0 3 4 2 2 9 16 17
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 1 5 0 1 0 0 0 0 0 0 0
SpindlePole 0 1 9 0 1 2 6 – 3 5 5 7 0 2 2 0 3 0 4 0 1 3 2 3
Vac/Vac Membrane 2 8 0 1 2 1 10 – 4 2 2 7 2 8 16 11 11 12 13 10 16 17 29 42
Unique Cell Count 190 251 284 155 168 254 364 294 314 395 525 309 348 382 139 197 206 207 249 291 194 300 240
Labelled Cell Count 215 265 295 164 175 352 396 405 409 546 680 311 358 391 151 213 221 207 249 291 194 300 240


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.3 6.0 6.0 6.0 6.0 4.0 5.2 – 4.5 4.8 4.5 4.6 6.1 6.5 6.3 8.3 7.7 8.2 5.7 5.9 6.2
Std Deviation (1e-4) 0.9 0.9 1.3 1.6 1.7 1.2 1.7 – 1.6 1.7 1.4 1.2 1.2 1.8 1.8 2.2 1.8 2.5 1.6 1.5 1.9
Intensity Change (Log2) – – – -0.01 0.0 -0.6 -0.22 – -0.42 -0.32 -0.42 -0.4 0.02 0.12 0.07 0.46 0.36 0.44 -0.08 -0.05 0.03


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cytoplasm 2.0 2.0 2.9 2.5 – 1.7 2.9 0.7 1.1 1.9 5.0 5.4 4.3 2.0 1.0
Endoplasmic Reticulum 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 4.9 – 0 0 0 0 0 0 0 0 0 0
Nucleus -1.8 -2.9 -6.1 -4.8 – -6.2 -6.7 -6.0 -6.2 -1.9 -5.4 -6.8 -5.9 -4.9 -2.8
Nuclear Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 -1.3 – 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 – 0 0 0 0 0 0 3.5 0 0 4.1

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 0.223 0.1663 -0.0054 -0.2183 -0.408 0.0397 2.0644 1.7956 1.5974 1.3416 1.2517 1.7331 4.5449 5.0127 4.6015 4.4133 4.1341 4.4944
Actin 0.0135 0 0.0012 0 0.0001 0.0007 0.0054 0 0.0019 0.0304 0.0147 0.0065 0 0 0 0 0.0012 0
Bud 0.0003 0 0.0001 0 0.0001 0 0.0005 0 0.0005 0.0016 0.0001 0.0002 0 0 0 0 0.0003 0
Bud Neck 0.0021 0 0.0007 0 0.0002 0.0008 0.0023 0 0.0012 0.0031 0.0011 0.0014 0.0002 0.0001 0 0 0.001 0.0005
Bud Periphery 0.0012 0 0.0002 0 0.0001 0.0001 0.001 0 0.0007 0.0038 0.0003 0.0008 0 0 0 0 0.0006 0
Bud Site 0.0004 0 0.0001 0 0.0002 0.0001 0.0043 0 0.0035 0.002 0.0003 0.0003 0.0001 0 0 0 0.0006 0
Cell Periphery 0.0002 0 0.0001 0 0 0 0.0008 0 0.0003 0.0001 0 0.0001 0 0 0 0 0.0001 0
Cytoplasm 0.0018 0.0002 0.001 0.0001 0.0037 0.0006 0.0073 0.0031 0.0019 0.0003 0.0002 0.0004 0.0004 0.001 0.0004 0.0003 0.0024 0.0002
Cytoplasmic Foci 0.0026 0 0.0005 0 0.0034 0.0019 0.0168 0.0002 0.0019 0.0051 0.0041 0.0014 0.0005 0 0 0 0.0034 0
Eisosomes 0.0002 0 0.0001 0 0 0 0.0001 0 0.0001 0.0002 0.0001 0 0 0 0 0 0 0
Endoplasmic Reticulum 0.0014 0 0.0005 0 0.0004 0.0003 0.0026 0.0002 0.001 0.0001 0.0005 0.0011 0.0001 0 0 0 0.0012 0
Endosome 0.0143 0 0.0005 0 0.0265 0.0036 0.0298 0.0002 0.0095 0.002 0.0031 0.0081 0.0006 0 0 0 0.0036 0
Golgi 0.0031 0 0.0003 0 0.0014 0.0007 0.012 0 0.0025 0.003 0.0036 0.0063 0.0001 0 0 0 0.0006 0
Lipid Particles 0.0048 0 0.0056 0 0.0005 0.005 0.0478 0 0.0016 0.0041 0.0195 0.0007 0.0004 0 0 0 0.0007 0
Mitochondria 0.0051 0.0002 0.004 0 0.0014 0.001 0.0152 0.0001 0.0051 0.0155 0.0072 0.0109 0.0001 0.0001 0.0001 0 0.0009 0
None 0.0021 0 0.0004 0 0.0016 0.0003 0.0012 0.004 0.0014 0.0005 0.0001 0.0001 0.0011 0.0012 0.0053 0.0003 0.0004 0
Nuclear Periphery 0.0179 0.0105 0.008 0.0008 0.0244 0.007 0.0272 0.0047 0.0125 0.0008 0.0253 0.0083 0.0013 0.0028 0.0012 0.0006 0.0026 0.0004
Nucleolus 0.0111 0.0063 0.005 0.0023 0.0195 0.0195 0.0205 0.0049 0.0052 0.0024 0.0305 0.0247 0.0068 0.0062 0.0044 0.0033 0.0134 0.0168
Nucleus 0.9053 0.9819 0.9682 0.9965 0.9105 0.9507 0.7711 0.9808 0.941 0.9133 0.8442 0.9185 0.9833 0.9838 0.9877 0.9941 0.9612 0.9806
Peroxisomes 0.0008 0 0.0011 0 0.0003 0.0011 0.0117 0 0.0005 0.0094 0.0217 0.0023 0.0001 0 0 0 0.0005 0
Punctate Nuclear 0.0077 0.0007 0.0016 0.0001 0.0031 0.0058 0.0121 0.0015 0.0025 0.0018 0.0215 0.0046 0.0047 0.0044 0.0007 0.0012 0.0042 0.0013
Vacuole 0.0024 0 0.0005 0 0.0018 0.0004 0.0079 0.0001 0.0036 0.0002 0.0007 0.0016 0.0002 0.0001 0 0 0.001 0
Vacuole Periphery 0.0017 0.0001 0.0003 0 0.0009 0.0003 0.0026 0 0.0015 0.0002 0.0013 0.0013 0 0 0 0 0.0002 0

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 8.5716 11.1455 12.4057 15.2617 13.1342 23.3643 15.8473 10.4582 16.6254 14.4999
Translational Efficiency 3.0542 1.5844 1.709 1.7249 1.7603 1.0331 1.3567 2.0807 1.4823 1.5917

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
2018 1268 245 1723 1636 1830 356 113 3654 3098 601 1836

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 752.83 800.57 1007.04 934.21 722.41 835.23 959.58 1027.19 739.21 821.04 978.93 939.93
Standard Deviation 97.43 111.42 118.87 140.86 89.46 117.21 119.31 130.02 95.15 116.13 121.39 141.98
Intensity Change Log 2 — 0.088703 0.419725 0.311423 — 0.209356 0.409585 0.507813 — 0.149047 0.414769 0.410934

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000122 0.000650 0.001159 0.001679 0.000132 0.000243 0.000777 0.001146 0.000126 0.000409 0.000933 0.001646
Bud Neck 0.002571 0.010553 0.006499 0.021850 0.001890 0.005342 0.008633 0.006536 0.002266 0.007475 0.007763 0.020907
Bud Site 0.000572 0.002277 0.000812 0.006825 0.000570 0.001216 0.000678 0.001256 0.000571 0.001650 0.000732 0.006482
Cell Periphery 0.000102 0.000214 0.000048 0.000140 0.000071 0.000116 0.000043 0.000220 0.000088 0.000156 0.000045 0.000145
Cytoplasm 0.009283 0.010003 0.000854 0.014929 0.008568 0.004583 0.001322 0.006958 0.008963 0.006801 0.001131 0.014439
Cytoplasmic Foci 0.000935 0.001285 0.000047 0.000542 0.001388 0.001842 0.000018 0.000072 0.001138 0.001614 0.000030 0.000513
Eisosomes 0.000011 0.000022 0.000040 0.000018 0.000011 0.000015 0.000025 0.000034 0.000011 0.000018 0.000031 0.000019
Endoplasmic Reticulum 0.002481 0.009301 0.008522 0.006138 0.002496 0.004123 0.005769 0.008824 0.002488 0.006242 0.006891 0.006303
Endosome 0.000248 0.000803 0.000206 0.001131 0.000246 0.001038 0.000132 0.000513 0.000247 0.000941 0.000162 0.001093
Golgi 0.000086 0.000208 0.000067 0.000752 0.000179 0.001004 0.000055 0.000090 0.000128 0.000678 0.000060 0.000711
Lipid Particles 0.000286 0.000637 0.000201 0.000220 0.000435 0.000508 0.000085 0.000197 0.000353 0.000561 0.000132 0.000219
Mitochondria 0.002089 0.002003 0.000887 0.007265 0.000892 0.003937 0.000777 0.001069 0.001553 0.003145 0.000821 0.006884
Mitotic Spindle 0.001128 0.002116 0.000066 0.009971 0.001353 0.002150 0.000197 0.000443 0.001229 0.002136 0.000143 0.009385
None 0.005057 0.007619 0.000978 0.006199 0.006135 0.004466 0.003517 0.006029 0.005540 0.005756 0.002482 0.006188
Nuclear Periphery 0.000921 0.001088 0.000373 0.002247 0.000739 0.001334 0.000334 0.000943 0.000839 0.001233 0.000350 0.002166
Nuclear Periphery Foci 0.000353 0.000592 0.000092 0.000496 0.000255 0.000518 0.000061 0.000261 0.000309 0.000548 0.000074 0.000482
Nucleolus 0.008115 0.013437 0.006435 0.002435 0.005592 0.008131 0.006807 0.013545 0.006986 0.010303 0.006656 0.003119
Nucleus 0.964658* 0.934382* 0.969910* 0.911771* 0.967937* 0.956635* 0.969478* 0.948302* 0.966126* 0.947527* 0.969654* 0.914019*
Peroxisomes 0.000406 0.001089 0.000297 0.000135 0.000540 0.000760 0.000197 0.000296 0.000466 0.000895 0.000238 0.000145
Vacuole 0.000422 0.001324 0.002402 0.004230 0.000467 0.001345 0.001016 0.003102 0.000442 0.001336 0.001581 0.004160
Vacuole Periphery 0.000152 0.000397 0.000107 0.001027 0.000103 0.000697 0.000081 0.000164 0.000130 0.000574 0.000092 0.000974

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -7.55 -11.49 -2.36 -1.30 0.12 -2.80 -12.88 -6.48 -6.03 -3.34 -7.51 -16.97 -2.58 -1.97 -0.45
Bud Neck -7.60 -4.46 -16.12 -9.19 -8.51 -6.49 -7.85 -6.77 -3.79 0.40 -9.69 -8.71 -16.73 -12.42 -9.41
Bud Site -2.54 -2.54 -5.34 -2.81 -4.72 -1.62 -1.42 -3.51 -2.35 -3.12 -3.05 -2.68 -5.51 -3.96 -4.98
Cell Periphery -3.22 3.07 -0.82 2.90 -5.17 -1.74 2.56 -1.29 -0.79 -1.54 -3.18 4.00 -2.26 1.49 -5.79
Cytoplasm -0.45 8.43 -1.84 -1.22 -9.55 3.01 7.21 -0.31 -0.99 -1.62 2.12 10.99 -2.20 -3.77 -9.48
Cytoplasmic Foci -0.51 3.54 2.41 2.25 -2.72 -0.47 3.15 2.78 3.34 -1.97 -0.88 4.61 3.39 3.75 -2.99
Eisosomes -7.25 -9.84 -11.56 -0.49 5.94 -2.64 -9.00 -6.09 -5.64 -3.54 -7.23 -13.34 -12.83 -5.82 4.88
Endoplasmic Reticulum -12.46 -11.26 -14.56 3.37 2.70 -5.79 -9.20 -8.02 -6.52 -4.36 -13.36 -14.32 -16.43 -3.30 -0.13
Endosome -3.06 0.19 -2.49 -0.08 -2.61 -3.28 1.38 -2.83 0.82 -3.38 -4.32 1.21 -2.69 0.46 -2.98
Golgi -1.59 0.20 -1.42 -0.88 -1.45 -2.02 1.03 0.18 2.13 -2.56 -2.31 1.06 -1.24 0.77 -1.49
Lipid Particles -1.55 1.18 1.43 1.90 0.55 -0.21 3.32 0.61 0.70 -4.18 -1.39 3.84 2.30 2.56 -1.43
Mitochondria 0.03 1.47 -2.53 -3.00 -4.05 -3.07 -0.27 -1.78 2.61 -3.14 -2.31 1.36 -3.29 -1.63 -4.13
Mitotic Spindle -0.92 1.85 -5.18 -4.16 -5.95 -0.92 1.54 -1.02 -0.92 -1.14 -1.36 2.33 -5.30 -4.67 -5.98
None -2.12 6.50 -0.01 2.18 -7.40 1.38 2.63 0.73 -0.20 -1.02 -0.22 4.85 0.67 0.84 -4.27
Nuclear Periphery -1.47 4.64 -6.66 -5.98 -8.14 -1.98 2.76 -3.13 -1.12 -3.99 -2.17 5.41 -7.17 -5.08 -8.49
Nuclear Periphery Foci -1.23 1.68 -1.17 0.14 -3.52 -1.49 2.02 -1.68 -0.93 -2.17 -1.74 2.44 -1.91 -0.26 -4.02
Nucleolus -4.39 1.61 5.45 9.23 3.13 -2.79 -1.95 -1.53 -0.73 -0.77 -4.51 -0.41 4.89 9.25 4.17
Nucleus 7.06 -0.13 11.79 4.54 11.84 2.71 0.97 3.64 2.74 3.40 6.29 0.58 13.09 8.07 12.87
Peroxisomes -1.53 0.48 1.36 2.43 5.11 -0.42 2.03 1.18 1.25 -2.55 -1.42 1.70 2.46 3.09 4.07
Vacuole -4.35 -7.66 -7.80 -6.44 -3.89 -2.72 -3.80 -3.94 -3.60 -3.50 -4.57 -8.05 -8.40 -7.03 -5.79
Vacuole Periphery -3.47 1.17 -2.11 -0.95 -2.27 -3.69 0.34 -2.55 2.90 -3.25 -4.47 1.35 -2.25 -0.06 -2.38
Standard name
Human Ortholog
Description Member of the NineTeen Complex (NTC); NTC contains Prp19p and stabilizes U6 snRNA in catalytic forms of spliceosome containing U2, U5, and U6 snRNAs; interacts with Prp16p to modulate splicing fidelity; isy1 syf2 cells have defective spindles
Localization
Cell Percentages nucleus (99%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-4

Isy1

Isy1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Isy1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available