Standard name | |
---|---|
Human Ortholog | |
Description | Nucleotide excision repair (NER) protein; binds damaged DNA during NER; binds DNA in an ATP-dependent manner (with Rad16p) during NER; required for repair of non-transcribed chromatin; subunit of Nucleotide Excision Repair Factor 4 (NEF4) and the Elongin-Cullin-Socs (ECS) ligase complex |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 1 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 2 | 1 | 0 | 0 | 1 |
Bud | 2 | 0 | 0 | 0 | 0 | 1 | 0 | – | 3 | 3 | 5 | 4 | 1 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 1 | 0 | 0 | 3 |
Bud Neck | 1 | 0 | 10 | 4 | 2 | 1 | 7 | – | 3 | 3 | 1 | 4 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 1 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 1 | 0 | – | 10 | 7 | 10 | 7 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 3 | 0 | 1 | 2 | 0 | 0 | 1 | – | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 |
Cytoplasm | 128 | 41 | 185 | 230 | 180 | 194 | 150 | – | 99 | 87 | 103 | 110 | 113 | 117 | 126 | 14 | 6 | 10 | 112 | 42 | 115 | 27 | 48 | 79 |
Endoplasmic Reticulum | 0 | 0 | 0 | 1 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 2 | 0 | 1 | 5 | 3 | 4 | 7 | 0 | 2 | 0 | 0 | 0 |
Endosome | 5 | 0 | 1 | 0 | 1 | 0 | 0 | – | 3 | 3 | 1 | 0 | 0 | 1 | 0 | 2 | 5 | 0 | 2 | 1 | 1 | 0 | 0 | 1 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 2 | 1 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 26 | 1 | 0 | 4 | 3 | 44 | 63 | – | 111 | 83 | 200 | 105 | 0 | 0 | 1 | 12 | 14 | 5 | 31 | 11 | 31 | 0 | 1 | 4 |
Nucleus | 27 | 15 | 27 | 34 | 60 | 123 | 142 | – | 181 | 170 | 217 | 230 | 19 | 20 | 30 | 211 | 191 | 180 | 35 | 21 | 31 | 4 | 20 | 14 |
Nuclear Periphery | 3 | 0 | 0 | 0 | 0 | 0 | 0 | – | 2 | 0 | 3 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 1 | 0 | 0 | 0 | 1 | 0 | 2 | – | 9 | 7 | 24 | 12 | 0 | 0 | 0 | 3 | 4 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 2 | 0 | 1 | 1 | 4 | 0 | 7 | – | 5 | 10 | 3 | 13 | 0 | 0 | 2 | 2 | 2 | 0 | 5 | 1 | 2 | 2 | 2 | 4 |
Vac/Vac Membrane | 14 | 2 | 4 | 4 | 17 | 24 | 11 | – | 5 | 6 | 10 | 8 | 1 | 5 | 3 | 13 | 11 | 13 | 25 | 12 | 25 | 3 | 10 | 11 |
Unique Cell Count | 185 | 57 | 218 | 268 | 260 | 356 | 338 | 352 | 317 | 426 | 413 | 135 | 140 | 159 | 244 | 216 | 200 | 231 | 98 | 228 | 41 | 91 | 129 | |
Labelled Cell Count | 212 | 59 | 230 | 280 | 268 | 388 | 383 | 432 | 379 | 577 | 494 | 136 | 143 | 164 | 264 | 237 | 215 | 231 | 98 | 228 | 41 | 91 | 129 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 3.5 | 5.0 | 5.1 | 4.7 | 5.3 | 4.3 | 4.4 | – | 4.2 | 4.4 | 3.9 | 4.2 | 5.1 | 5.3 | 5.3 | 8.0 | 7.6 | 7.2 | – | 4.8 | 5.5 |
Std Deviation (1e-4) | 0.6 | 1.3 | 1.0 | 1.4 | 1.4 | 0.9 | 1.3 | – | 1.0 | 1.2 | 1.3 | 1.2 | 1.7 | 1.3 | 1.5 | 1.6 | 1.5 | 1.5 | – | 1.3 | 1.9 |
Intensity Change (Log2) | – | – | – | -0.1 | 0.05 | -0.25 | -0.19 | – | -0.27 | -0.21 | -0.37 | -0.26 | 0.02 | 0.06 | 0.05 | 0.65 | 0.59 | 0.51 | – | -0.08 | 0.11 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | -2.7 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0.3 | -4.0 | -7.5 | -9.5 | – | -13.2 | -13.1 | -14.7 | -13.9 | -0.3 | -0.3 | -1.4 | -17.1 | -17.2 | -16.3 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3.3 | 3.8 | 0 |
Nucleus | 0.1 | 3.0 | 5.9 | 7.4 | – | 9.4 | 9.7 | 9.5 | 10.5 | 0.5 | 0.5 | 1.7 | 15.9 | 15.8 | 15.9 |
Nuclear Periphery | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 2.5 | 2.6 | 1.0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2.0 | 1.9 | 2.4 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | -0.5694 | -0.9179 | -0.7958 | -0.9099 | -1.2764 | -0.7836 | -0.9765 | -0.5805 | -0.9215 | -0.9372 | -1.3287 | -0.9759 | -0.2467 | -0.9938 | -0.8097 | -1.1097 | -0.7618 | -1.0241 |
Actin | 0.0473 | 0.0002 | 0.0016 | 0.0016 | 0.0007 | 0.0015 | 0.0131 | 0.0001 | 0.0131 | 0.0008 | 0.0011 | 0.0007 | 0.0542 | 0.002 | 0.0088 | 0.0319 | 0.1215 | 0.0005 |
Bud | 0.0007 | 0.0023 | 0.0002 | 0.0001 | 0.0019 | 0.0001 | 0.0012 | 0.0004 | 0.0009 | 0.0027 | 0.0001 | 0.0013 | 0.003 | 0.001 | 0.0007 | 0.0117 | 0.001 | 0.0002 |
Bud Neck | 0.0056 | 0.0004 | 0.0002 | 0.0002 | 0.0009 | 0.0016 | 0.0005 | 0.0008 | 0.0006 | 0.0014 | 0.0003 | 0.0011 | 0.0088 | 0.0003 | 0.0009 | 0.0019 | 0.0005 | 0.0011 |
Bud Periphery | 0.0016 | 0.0037 | 0.0003 | 0.0001 | 0.0013 | 0.0002 | 0.001 | 0.0002 | 0.0025 | 0.0023 | 0.0001 | 0.0052 | 0.0061 | 0.0029 | 0.0008 | 0.0062 | 0.0019 | 0.0002 |
Bud Site | 0.003 | 0.0061 | 0.0022 | 0.0001 | 0.0006 | 0.0002 | 0.0043 | 0.0087 | 0.0014 | 0.0332 | 0.0001 | 0.0005 | 0.0112 | 0.0203 | 0.013 | 0.0037 | 0.0014 | 0.0001 |
Cell Periphery | 0.0002 | 0.0001 | 0 | 0 | 0.0001 | 0 | 0.0003 | 0.0002 | 0.0001 | 0.0003 | 0 | 0.0001 | 0.0009 | 0.0002 | 0.0003 | 0.0004 | 0.0002 | 0 |
Cytoplasm | 0.0414 | 0.0435 | 0.0393 | 0.0339 | 0.0498 | 0.0671 | 0.0235 | 0.0174 | 0.044 | 0.0379 | 0.029 | 0.055 | 0.0417 | 0.067 | 0.0491 | 0.061 | 0.0431 | 0.1167 |
Cytoplasmic Foci | 0.0196 | 0.0105 | 0.004 | 0.0144 | 0.0093 | 0.0148 | 0.0117 | 0.0095 | 0.0099 | 0.0288 | 0.0026 | 0.0072 | 0.0421 | 0.0046 | 0.0133 | 0.0175 | 0.0112 | 0.0172 |
Eisosomes | 0.0009 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0.0004 | 0.0001 | 0.0002 | 0.0001 | 0.0002 | 0.0001 | 0.0006 | 0.0002 | 0.0002 | 0.0004 | 0.0005 | 0.0001 |
Endoplasmic Reticulum | 0.0038 | 0.0011 | 0.0011 | 0.0008 | 0.0028 | 0.0015 | 0.0025 | 0.0003 | 0.0018 | 0.0008 | 0.0004 | 0.0004 | 0.0052 | 0.0007 | 0.0013 | 0.0025 | 0.0026 | 0.001 |
Endosome | 0.0097 | 0.0011 | 0.001 | 0.0184 | 0.0029 | 0.0021 | 0.0215 | 0.0008 | 0.0166 | 0.0056 | 0.0024 | 0.0055 | 0.0302 | 0.0007 | 0.0115 | 0.0091 | 0.0066 | 0.003 |
Golgi | 0.0052 | 0.0001 | 0.0001 | 0.0004 | 0.0002 | 0.0002 | 0.0063 | 0.0003 | 0.0055 | 0.0024 | 0.002 | 0.0007 | 0.0093 | 0.0002 | 0.0048 | 0.006 | 0.0177 | 0.0002 |
Lipid Particles | 0.0092 | 0.0007 | 0.0004 | 0.0016 | 0.0006 | 0.0006 | 0.0067 | 0.0063 | 0.0037 | 0.0102 | 0.0045 | 0.0093 | 0.0308 | 0.0003 | 0.0046 | 0.0082 | 0.0568 | 0.0007 |
Mitochondria | 0.0021 | 0.0006 | 0.0004 | 0.0005 | 0.0013 | 0.0005 | 0.0352 | 0.0004 | 0.0016 | 0.002 | 0.0788 | 0.0015 | 0.0148 | 0.0009 | 0.0109 | 0.0107 | 0.012 | 0.0006 |
None | 0.6718 | 0.8021 | 0.8318 | 0.7207 | 0.7252 | 0.7657 | 0.7534 | 0.8222 | 0.7514 | 0.6282 | 0.6707 | 0.7536 | 0.5437 | 0.8124 | 0.7393 | 0.7393 | 0.5709 | 0.6433 |
Nuclear Periphery | 0.0093 | 0.0038 | 0.0022 | 0.0048 | 0.0119 | 0.0041 | 0.0101 | 0.0016 | 0.0027 | 0.0026 | 0.0022 | 0.0011 | 0.0126 | 0.0008 | 0.0034 | 0.0083 | 0.0018 | 0.0027 |
Nucleolus | 0.0021 | 0.0029 | 0.0015 | 0.003 | 0.0047 | 0.0021 | 0.0038 | 0.0057 | 0.0034 | 0.0049 | 0.0033 | 0.0027 | 0.0053 | 0.0007 | 0.0016 | 0.0039 | 0.0044 | 0.0022 |
Nucleus | 0.112 | 0.1048 | 0.0976 | 0.1726 | 0.1389 | 0.1233 | 0.0876 | 0.1049 | 0.1208 | 0.2021 | 0.1829 | 0.1342 | 0.1238 | 0.0803 | 0.1064 | 0.0589 | 0.1019 | 0.1848 |
Peroxisomes | 0.0099 | 0.0005 | 0.0003 | 0.0033 | 0.0004 | 0.0011 | 0.0055 | 0.0005 | 0.005 | 0.0108 | 0.0056 | 0.0013 | 0.0346 | 0.0012 | 0.013 | 0.007 | 0.0397 | 0.0008 |
Punctate Nuclear | 0.0425 | 0.0145 | 0.0149 | 0.0199 | 0.0432 | 0.0122 | 0.0085 | 0.0188 | 0.0138 | 0.0184 | 0.0134 | 0.0165 | 0.0105 | 0.0024 | 0.0122 | 0.0063 | 0.0025 | 0.0225 |
Vacuole | 0.0015 | 0.0008 | 0.0004 | 0.0027 | 0.0026 | 0.0007 | 0.0017 | 0.0008 | 0.001 | 0.0041 | 0.0002 | 0.0018 | 0.008 | 0.0011 | 0.0036 | 0.0038 | 0.0013 | 0.0016 |
Vacuole Periphery | 0.0006 | 0.0002 | 0.0001 | 0.0007 | 0.0007 | 0.0002 | 0.0012 | 0.0001 | 0.0002 | 0.0005 | 0.0002 | 0.0002 | 0.0026 | 0.0001 | 0.0003 | 0.0013 | 0.0005 | 0.0003 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 7.473 | 5.809 | 6.0348 | 9.808 | 7.5827 | 5.8452 | 9.6913 | 10.9016 | 11.0707 | 10.8481 |
Translational Efficiency | 1.0364 | 1.6624 | 1.2989 | 0.9118 | 1.2055 | 2.0837 | 1.1621 | 1.0087 | 1.0473 | 1.0746 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
943 | 1437 | 420 | 664 | 1713 | 1838 | 285 | 762 | 2656 | 3275 | 705 | 1426 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 612.77 | 691.19 | 951.92 | 824.13 | 663.96 | 687.24 | 896.59 | 798.69 | 645.79 | 688.97 | 929.55 | 810.54 |
Standard Deviation | 85.56 | 107.49 | 109.20 | 106.88 | 75.15 | 90.68 | 93.99 | 112.99 | 82.71 | 98.43 | 106.83 | 110.91 |
Intensity Change Log 2 | — | 0.173737 | 0.635495 | 0.427526 | — | 0.049718 | 0.433352 | 0.266539 | — | 0.110573 | 0.533909 | 0.346050 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000182 | 0.000205 | 0.000902 | 0.000609 | 0.000059 | 0.000288 | 0.000258 | 0.000409 | 0.000103 | 0.000251 | 0.000642 | 0.000502 |
Bud Neck | 0.002556 | 0.024643 | 0.005251 | 0.013813 | 0.011071 | 0.033530 | 0.001131 | 0.006798 | 0.008048 | 0.029631 | 0.003586 | 0.010065 |
Bud Site | 0.001639 | 0.005712 | 0.010498 | 0.019085 | 0.002153 | 0.014047 | 0.000934 | 0.021086 | 0.001970 | 0.010390 | 0.006632 | 0.020155 |
Cell Periphery | 0.000291 | 0.000181 | 0.000229 | 0.000205 | 0.000126 | 0.000252 | 0.000150 | 0.000228 | 0.000185 | 0.000221 | 0.000197 | 0.000217 |
Cytoplasm | 0.073762 | 0.387368* | 0.099236 | 0.275877* | 0.476902* | 0.355885* | 0.169538* | 0.360283* | 0.333769* | 0.369699* | 0.127656* | 0.320980* |
Cytoplasmic Foci | 0.010735 | 0.186676* | 0.007407 | 0.009769 | 0.171604 | 0.182800* | 0.028350 | 0.010445 | 0.114488 | 0.184501* | 0.015873 | 0.010130 |
Eisosomes | 0.000041 | 0.000134 | 0.000098 | 0.000056 | 0.000096 | 0.000138 | 0.000090 | 0.000052 | 0.000077 | 0.000136 | 0.000095 | 0.000054 |
Endoplasmic Reticulum | 0.001677 | 0.000873 | 0.009853 | 0.005537 | 0.000645 | 0.000897 | 0.003884 | 0.003807 | 0.001012 | 0.000886 | 0.007440 | 0.004613 |
Endosome | 0.000691 | 0.011488 | 0.003897 | 0.003114 | 0.005742 | 0.022915 | 0.003459 | 0.004785 | 0.003949 | 0.017901 | 0.003720 | 0.004006 |
Golgi | 0.000104 | 0.001803 | 0.000120 | 0.006503 | 0.001355 | 0.004248 | 0.000073 | 0.001943 | 0.000911 | 0.003175 | 0.000101 | 0.004067 |
Lipid Particles | 0.002659 | 0.008473 | 0.003534 | 0.001691 | 0.006879 | 0.010471 | 0.004068 | 0.001379 | 0.005381 | 0.009594 | 0.003750 | 0.001524 |
Mitochondria | 0.001219 | 0.008544 | 0.000988 | 0.001516 | 0.002581 | 0.017223 | 0.000359 | 0.001998 | 0.002097 | 0.013415 | 0.000734 | 0.001773 |
Mitotic Spindle | 0.001193 | 0.002084 | 0.001330 | 0.011520 | 0.003751 | 0.008238 | 0.001149 | 0.029307 | 0.002843 | 0.005538 | 0.001257 | 0.021024 |
None | 0.024557 | 0.011483 | 0.002911 | 0.006889 | 0.004548 | 0.006811 | 0.004335 | 0.013174 | 0.011652 | 0.008861 | 0.003487 | 0.010247 |
Nuclear Periphery | 0.001055 | 0.000649 | 0.002310 | 0.002202 | 0.000584 | 0.000808 | 0.001708 | 0.001819 | 0.000751 | 0.000738 | 0.002066 | 0.001997 |
Nuclear Periphery Foci | 0.000517 | 0.000467 | 0.001753 | 0.001447 | 0.000333 | 0.000301 | 0.001055 | 0.000896 | 0.000398 | 0.000374 | 0.001471 | 0.001152 |
Nucleolus | 0.013046 | 0.003520 | 0.002378 | 0.000551 | 0.001412 | 0.004616 | 0.001139 | 0.001002 | 0.005542 | 0.004135 | 0.001877 | 0.000792 |
Nucleus | 0.860705* | 0.291500* | 0.781931* | 0.564821* | 0.278464* | 0.268657* | 0.720171* | 0.461366* | 0.485186* | 0.278680* | 0.756964* | 0.509539* |
Peroxisomes | 0.000659 | 0.010953 | 0.000569 | 0.000551 | 0.003301 | 0.015260 | 0.000480 | 0.001161 | 0.002363 | 0.013370 | 0.000533 | 0.000877 |
Vacuole | 0.002514 | 0.042214 | 0.064478 | 0.071461 | 0.027918 | 0.049999 | 0.057480 | 0.076310 | 0.018899 | 0.046583 | 0.061649 | 0.074052 |
Vacuole Periphery | 0.000199 | 0.001030 | 0.000329 | 0.002782 | 0.000474 | 0.002615 | 0.000189 | 0.001752 | 0.000376 | 0.001919 | 0.000273 | 0.002231 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -0.28 | -8.66 | -4.22 | -5.17 | 4.30 | -3.84 | -8.08 | -11.73 | -3.10 | -2.43 | -3.33 | -11.83 | -8.98 | -5.82 | 4.00 |
Bud Neck | -10.85 | -5.08 | -5.19 | 5.45 | -2.44 | -10.06 | 7.62 | 2.30 | 12.66 | -7.23 | -13.57 | 3.74 | -0.93 | 12.74 | -4.75 |
Bud Site | -3.81 | -2.45 | -3.96 | -2.44 | -0.48 | -8.31 | 2.23 | -4.99 | 0.15 | -5.36 | -9.25 | -2.26 | -6.37 | -1.28 | -2.51 |
Cell Periphery | 2.87 | 0.98 | 2.90 | 0.00 | 3.69 | -4.18 | -1.95 | -2.24 | 2.54 | -0.67 | -1.57 | -1.55 | 0.59 | 2.36 | 2.66 |
Cytoplasm | -33.79* | -1.66 | -12.26 | 15.93 | -9.98 | 13.05 | 24.02 | 18.94 | 8.09 | -7.06 | -4.44 | 25.29 | 11.55 | 16.37 | -12.83 |
Cytoplasmic Foci | -30.85* | 2.50 | 2.18 | 32.34 | -0.44 | -1.67 | 25.51 | 34.69 | 35.61 | 3.88 | -14.19 | 27.31 | 31.09 | 48.12 | 3.23 |
Eisosomes | -10.86 | -9.25 | -6.87 | 6.45 | 4.69 | -4.74 | -1.43 | 5.40 | 7.78 | 4.71 | -9.67 | -6.63 | -0.01 | 10.02 | 6.90 |
Endoplasmic Reticulum | 4.59 | -14.72 | -13.45 | -15.45 | 5.05 | -2.08 | -9.59 | -14.60 | -13.74 | -0.46 | 1.24 | -17.20 | -19.88 | -20.46 | 5.07 |
Endosome | -11.81 | -10.09 | -6.75 | 9.43 | 4.42 | -11.81 | 3.45 | 3.85 | 13.40 | 0.96 | -15.12 | -0.84 | 2.54 | 16.21 | 3.44 |
Golgi | -9.47 | -2.55 | -1.56 | -0.20 | -1.52 | -4.82 | 3.90 | 1.18 | 5.18 | -1.28 | -6.25 | 3.74 | -0.60 | 2.65 | -1.89 |
Lipid Particles | -8.25 | -2.49 | 2.25 | 10.76 | 5.88 | -3.77 | 4.01 | 8.59 | 12.11 | 5.05 | -6.61 | 3.04 | 8.75 | 16.03 | 7.92 |
Mitochondria | -5.99 | 1.00 | 0.47 | 6.07 | -0.46 | -9.14 | 4.06 | 1.74 | 10.21 | -3.61 | -10.64 | 3.69 | 1.96 | 11.87 | -2.86 |
Mitotic Spindle | -0.70 | -1.34 | -4.05 | -3.88 | -2.82 | -2.32 | 1.84 | -4.20 | -3.11 | -5.07 | -2.09 | 0.08 | -5.61 | -4.72 | -5.38 |
None | 4.32 | 8.43 | 7.32 | 3.41 | -3.93 | -2.59 | 1.05 | -2.65 | -0.89 | -3.19 | 2.23 | 8.06 | 4.09 | 1.94 | -4.81 |
Nuclear Periphery | 3.74 | -12.05 | -13.60 | -16.64 | -3.05 | -2.96 | -12.73 | -14.59 | -12.70 | -5.44 | -0.11 | -18.61 | -20.58 | -20.13 | -5.37 |
Nuclear Periphery Foci | 0.36 | -6.17 | -4.16 | -5.77 | 2.31 | 0.94 | -6.46 | -4.69 | -4.94 | -0.35 | 0.54 | -9.14 | -6.72 | -7.57 | 2.07 |
Nucleolus | 8.86 | 10.79 | 12.53 | 7.53 | 6.66 | -6.36 | 1.67 | 1.96 | 8.23 | 0.53 | 2.76 | 9.20 | 11.76 | 11.09 | 5.69 |
Nucleus | 52.49* | 7.48 | 20.71 | -20.93 | 10.07 | 1.05 | -22.99 | -18.72 | -18.53 | 8.72 | 23.36 | -19.39 | -5.62 | -27.73 | 13.95 |
Peroxisomes | -9.67 | 1.27 | 2.21 | 9.96 | 1.33 | -9.99 | 5.51 | 4.86 | 13.41 | -0.25 | -13.19 | 5.53 | 5.29 | 16.72 | 0.37 |
Vacuole | -17.18 | -12.05 | -18.46 | -12.70 | -4.44 | -8.77 | -7.28 | -15.01 | -11.85 | -3.56 | -15.63 | -12.86 | -22.89 | -17.28 | -5.69 |
Vacuole Periphery | -10.38 | -4.15 | -1.35 | 0.14 | -1.10 | -9.13 | 5.95 | -0.58 | 4.56 | -1.38 | -11.21 | 2.85 | -1.33 | 3.20 | -1.65 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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