Standard name | |
---|---|
Human Ortholog | |
Description | Subunit of a two-component GTPase-activating protein, Bfa1p-Bub2p; contributes to GAP activity, inactivating Tem1 by stimulating GTP hydrolysis following damage or misalignment of the mitotic spindle; functions as a guanine-nucleotide exchange inhibitor (GDI) for Tem1p; involved in multiple cell cycle checkpoint pathways that control mitotic exit; required when telomeres are damaged, but not for all types of chromosomal DNA damage; phosphorylated by the Polo-like kinase Cdc5p |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 1 | 1 | 2 | 4 | 4 | 5 | 3 | – | 1 | 3 | 3 | 2 | 1 | 1 | 0 | 1 | 0 | 1 | 2 | 1 | 1 | 0 | 1 | 4 |
Bud | 16 | 8 | 4 | 8 | 7 | 16 | 25 | – | 15 | 27 | 31 | 23 | 1 | 2 | 2 | 3 | 2 | 3 | 10 | 9 | 4 | 5 | 6 | 7 |
Bud Neck | 10 | 6 | 11 | 6 | 6 | 12 | 15 | – | 7 | 19 | 12 | 11 | 5 | 2 | 4 | 2 | 4 | 2 | 3 | 8 | 0 | 3 | 3 | 2 |
Bud Site | 30 | 3 | 1 | 2 | 1 | 11 | 15 | – | 17 | 19 | 21 | 28 | 0 | 0 | 1 | 4 | 5 | 1 | – | – | – | – | – | – |
Cell Periphery | 2 | 1 | 0 | 0 | 0 | 0 | 1 | – | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 63 | 33 | 22 | 30 | 22 | 25 | 36 | – | 25 | 30 | 32 | 20 | 40 | 62 | 72 | 27 | 35 | 20 | 25 | 16 | 9 | 36 | 85 | 116 |
Endoplasmic Reticulum | 0 | 1 | 0 | 0 | 1 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 36 | 51 | 4 | 1 | 2 | 7 | 6 | – | 4 | 2 | 2 | 3 | 8 | 14 | 31 | 16 | 23 | 20 | 16 | 18 | 6 | 4 | 5 | 3 |
Golgi | 0 | 1 | 0 | 1 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 2 | 1 | 2 | 1 | 1 | 0 | 0 | 0 |
Mitochondria | 19 | 10 | 1 | 6 | 2 | 13 | 16 | – | 27 | 21 | 43 | 35 | 0 | 0 | 0 | 6 | 7 | 4 | 4 | 5 | 7 | 0 | 1 | 4 |
Nucleus | 1 | 0 | 0 | 1 | 2 | 3 | 3 | – | 6 | 1 | 2 | 3 | 0 | 0 | 3 | 6 | 2 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
Nucleolus | 10 | 13 | 2 | 0 | 1 | 8 | 12 | – | 8 | 9 | 12 | 9 | 0 | 0 | 4 | 17 | 26 | 18 | 3 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 28 | 46 | 1 | 2 | 0 | 4 | 4 | – | 9 | 1 | 11 | 4 | 4 | 4 | 15 | 30 | 44 | 25 | 2 | 5 | 1 | 0 | 0 | 0 |
SpindlePole | 325 | 235 | 100 | 129 | 128 | 163 | 280 | – | 160 | 170 | 173 | 191 | 88 | 146 | 155 | 236 | 271 | 173 | 365 | 245 | 115 | 27 | 60 | 50 |
Vac/Vac Membrane | 5 | 0 | 1 | 0 | 0 | 3 | 1 | – | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 2 | 5 | 2 | 1 | 0 | 0 | 0 | 1 | 0 |
Unique Cell Count | 422 | 308 | 128 | 169 | 155 | 216 | 334 | 212 | 221 | 243 | 236 | 137 | 218 | 249 | 294 | 331 | 213 | 441 | 315 | 152 | 83 | 171 | 194 | |
Labelled Cell Count | 546 | 409 | 149 | 190 | 176 | 270 | 417 | 279 | 302 | 342 | 329 | 148 | 233 | 289 | 356 | 427 | 271 | 441 | 315 | 152 | 83 | 171 | 194 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 4.3 | 5.5 | 5.4 | 5.3 | 5.7 | 4.6 | 4.6 | – | 4.0 | 4.1 | 3.8 | 3.9 | 5.8 | 5.9 | 5.7 | 8.7 | 8.9 | 9.1 | 4.8 | 5.4 | 5.5 |
Std Deviation (1e-4) | 0.7 | 0.9 | 0.8 | 1.4 | 1.5 | 1.1 | 1.0 | – | 0.9 | 1.2 | 1.1 | 1.1 | 1.2 | 1.2 | 1.4 | 1.8 | 2.0 | 2.6 | 1.4 | 1.5 | 1.5 |
Intensity Change (Log2) | – | – | – | -0.05 | 0.06 | -0.25 | -0.23 | – | -0.43 | -0.39 | -0.51 | -0.49 | 0.09 | 0.12 | 0.07 | 0.69 | 0.72 | 0.75 | -0.19 | -0.0 | 0.02 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0.7 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | -1.9 | -1.7 | 0 | 0 | – | 0 | 0 | 0 | 0 | -1.7 | 0 | -3.3 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0.1 | -0.7 | -1.5 | -1.9 | – | -1.4 | -0.9 | -1.0 | -2.5 | 2.3 | 2.4 | 2.5 | -2.4 | -1.9 | -2.1 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 1.1 | 1.3 | 3.0 | 1.0 | 1.6 | 2.2 |
Golgi | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1.9 | 2.5 | 2.6 |
Peroxisomes | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 2.4 | 3.4 | 4.0 | 3.7 |
SpindlePole | -0.4 | 0.9 | 0 | 0 | – | 0 | 0 | 0 | 0 | -2.5 | -2.2 | -3.1 | 0.5 | 0.9 | 0.7 |
Vacuole | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | -0.4931 | 0.4294 | 0.268 | 0.2038 | 0.4585 | 0.1734 | 0.0922 | 0.4495 | 0.4511 | 0.4483 | 0.2324 | 0.5343 | 0.454 | 0.1198 | 0.0759 | 0.3301 | 0.4377 | 0.0401 |
Actin | 0.0012 | 0.0015 | 0.0008 | 0.0015 | 0.0005 | 0.0007 | 0.0087 | 0.0004 | 0.0057 | 0.0009 | 0.0031 | 0.0017 | 0.027 | 0.0019 | 0.0184 | 0.0001 | 0.0002 | 0.0003 |
Bud | 0.0001 | 0.0022 | 0.0004 | 0.0001 | 0.0005 | 0.0001 | 0.0026 | 0.0015 | 0.0017 | 0.0001 | 0.0025 | 0.0004 | 0.0077 | 0.0024 | 0.0015 | 0.0002 | 0.0001 | 0 |
Bud Neck | 0.0012 | 0.0045 | 0.0126 | 0.0653 | 0.0094 | 0.0051 | 0.0022 | 0.0042 | 0.0288 | 0.0972 | 0.0034 | 0.0065 | 0.0272 | 0.0011 | 0.0029 | 0.0026 | 0.0002 | 0.0007 |
Bud Periphery | 0.0001 | 0.0019 | 0.0004 | 0.0001 | 0.0011 | 0.0001 | 0.0024 | 0.0019 | 0.0009 | 0.0002 | 0.0038 | 0.0008 | 0.006 | 0.0048 | 0.0021 | 0.0001 | 0.0001 | 0 |
Bud Site | 0.0023 | 0.0244 | 0.0078 | 0.0018 | 0.0014 | 0.001 | 0.0037 | 0.0243 | 0.0045 | 0.0005 | 0.0099 | 0.0009 | 0.0156 | 0.008 | 0.0091 | 0.0003 | 0.0002 | 0.0002 |
Cell Periphery | 0 | 0.0002 | 0.0001 | 0 | 0.0001 | 0 | 0.0003 | 0.0002 | 0.0001 | 0 | 0.0001 | 0.0001 | 0.001 | 0.0001 | 0.0001 | 0 | 0 | 0 |
Cytoplasm | 0.0066 | 0.016 | 0.0022 | 0.0023 | 0.0013 | 0.0042 | 0.0066 | 0.0174 | 0.0026 | 0.0014 | 0.0019 | 0.013 | 0.0048 | 0.0416 | 0.0044 | 0.0005 | 0.0004 | 0.0062 |
Cytoplasmic Foci | 0.3086 | 0.1307 | 0.1863 | 0.2895 | 0.2875 | 0.1688 | 0.2676 | 0.1863 | 0.1315 | 0.2551 | 0.2244 | 0.1469 | 0.3122 | 0.2914 | 0.2859 | 0.3476 | 0.5318 | 0.2813 |
Eisosomes | 0 | 0.0001 | 0 | 0.0001 | 0.0002 | 0 | 0.0016 | 0.0001 | 0.0001 | 0 | 0 | 0.0001 | 0.0005 | 0.0001 | 0.0001 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0.0002 | 0.0017 | 0.0003 | 0 | 0.0001 | 0.0002 | 0.0018 | 0.0007 | 0.0008 | 0.0015 | 0.0002 | 0.0006 | 0.0037 | 0.0019 | 0.0003 | 0 | 0.0001 | 0.0001 |
Endosome | 0.0069 | 0.0246 | 0.0058 | 0.0069 | 0.0045 | 0.0123 | 0.0163 | 0.0147 | 0.0064 | 0.0131 | 0.0055 | 0.0106 | 0.0253 | 0.017 | 0.0062 | 0.0007 | 0.0036 | 0.0089 |
Golgi | 0.0021 | 0.0041 | 0.0019 | 0.0031 | 0.0036 | 0.0019 | 0.0036 | 0.0031 | 0.0026 | 0.003 | 0.0041 | 0.0021 | 0.014 | 0.0041 | 0.0023 | 0.0009 | 0.0034 | 0.0026 |
Lipid Particles | 0.032 | 0.0458 | 0.0234 | 0.0211 | 0.0702 | 0.0248 | 0.0378 | 0.0266 | 0.0127 | 0.0367 | 0.0505 | 0.0152 | 0.0445 | 0.047 | 0.0128 | 0.0047 | 0.0844 | 0.0415 |
Mitochondria | 0.0001 | 0.0011 | 0.0002 | 0.0001 | 0.0007 | 0.0004 | 0.0101 | 0.0005 | 0.0029 | 0.0002 | 0.001 | 0.0014 | 0.0133 | 0.0079 | 0.0016 | 0.0002 | 0.0002 | 0 |
None | 0.053 | 0.0768 | 0.0204 | 0.0241 | 0.0417 | 0.0431 | 0.0343 | 0.063 | 0.0204 | 0.0202 | 0.0451 | 0.0602 | 0.004 | 0.0622 | 0.0115 | 0.0007 | 0.0006 | 0.0147 |
Nuclear Periphery | 0.0004 | 0.0045 | 0.001 | 0.0003 | 0.0006 | 0.0012 | 0.0043 | 0.0023 | 0.0026 | 0.0115 | 0.0012 | 0.0048 | 0.0107 | 0.0012 | 0.0004 | 0.0002 | 0.0003 | 0.0003 |
Nucleolus | 0.0085 | 0.0322 | 0.0178 | 0.0011 | 0.0097 | 0.0098 | 0.0021 | 0.0199 | 0.0146 | 0.0083 | 0.005 | 0.013 | 0.0039 | 0.0053 | 0.0016 | 0.0015 | 0.0008 | 0.001 |
Nucleus | 0.0069 | 0.0234 | 0.008 | 0.0005 | 0.0012 | 0.0067 | 0.0023 | 0.0139 | 0.01 | 0.0069 | 0.0017 | 0.0076 | 0.0088 | 0.0014 | 0.001 | 0.0006 | 0.0001 | 0.0005 |
Peroxisomes | 0.0387 | 0.0417 | 0.0228 | 0.0298 | 0.1214 | 0.0184 | 0.0284 | 0.0284 | 0.0214 | 0.012 | 0.0916 | 0.0152 | 0.0297 | 0.0263 | 0.0154 | 0.0063 | 0.0341 | 0.0124 |
Punctate Nuclear | 0.5307 | 0.559 | 0.6865 | 0.5522 | 0.4427 | 0.7004 | 0.5613 | 0.5856 | 0.7274 | 0.5286 | 0.5431 | 0.6973 | 0.4288 | 0.4717 | 0.6217 | 0.6327 | 0.3392 | 0.6292 |
Vacuole | 0.0003 | 0.003 | 0.0011 | 0.0001 | 0.0013 | 0.0005 | 0.0014 | 0.0048 | 0.0019 | 0.0022 | 0.0015 | 0.0014 | 0.0093 | 0.0024 | 0.0007 | 0 | 0.0001 | 0.0001 |
Vacuole Periphery | 0 | 0.0007 | 0.0001 | 0 | 0.0001 | 0.0001 | 0.0004 | 0.0003 | 0.0004 | 0.0005 | 0.0002 | 0.0004 | 0.0021 | 0.0002 | 0.0001 | 0 | 0.0001 | 0 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 7.0362 | 13.1517 | 1.6972 | 3.1032 | 7.8787 | 3.8358 | 9.3951 | 6.6337 | 4.0738 | 10.5082 |
Translational Efficiency | – | – | – | – | – | – | – | – | – | – |
Dataset | Hit |
---|---|
Protein Concentration | ✔ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | – |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
432 | 662 | 1443 | 304 | 2079 | 1878 | 402 | 177 | 2511 | 2540 | 1845 | 481 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 571.77 | 710.56 | 960.44 | 885.01 | 713.04 | 714.83 | 933.62 | 892.52 | 688.74 | 713.72 | 954.60 | 887.77 |
Standard Deviation | 69.42 | 119.87 | 119.39 | 131.43 | 81.84 | 96.09 | 110.64 | 126.23 | 96.01 | 102.84 | 118.05 | 129.59 |
Intensity Change Log 2 | — | 0.313522 | 0.748261 | 0.630259 | — | 0.003617 | 0.388852 | 0.323901 | — | 0.149802 | 0.559927 | 0.468319 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.001608 | 0.000688 | 0.000358 | 0.002062 | 0.000158 | 0.000354 | 0.000399 | 0.000735 | 0.000407 | 0.000441 | 0.000367 | 0.001574 |
Bud Neck | 0.012784 | 0.008924 | 0.005315 | 0.019374 | 0.009412 | 0.018346 | 0.008413 | 0.004423 | 0.009992 | 0.015890 | 0.005990 | 0.013872 |
Bud Site | 0.010862 | 0.021989 | 0.022297 | 0.063107 | 0.011959 | 0.021115 | 0.041497 | 0.046599 | 0.011771 | 0.021343 | 0.026480 | 0.057032 |
Cell Periphery | 0.000387 | 0.000237 | 0.000074 | 0.000110 | 0.000152 | 0.000158 | 0.000082 | 0.000076 | 0.000192 | 0.000179 | 0.000076 | 0.000098 |
Cytoplasm | 0.081623 | 0.091583 | 0.026192 | 0.025463 | 0.069158 | 0.104863* | 0.041164 | 0.020433 | 0.071303 | 0.101402* | 0.029454 | 0.023612 |
Cytoplasmic Foci | 0.538583* | 0.418759* | 0.054373 | 0.130824 | 0.622023* | 0.487092* | 0.114496 | 0.205565* | 0.607668* | 0.469282* | 0.067473 | 0.158327 |
Eisosomes | 0.000129 | 0.000087 | 0.000021 | 0.000041 | 0.000050 | 0.000069 | 0.000032 | 0.000023 | 0.000064 | 0.000074 | 0.000024 | 0.000034 |
Endoplasmic Reticulum | 0.000744 | 0.000534 | 0.000783 | 0.000342 | 0.000222 | 0.000385 | 0.000452 | 0.000194 | 0.000312 | 0.000424 | 0.000711 | 0.000287 |
Endosome | 0.024560 | 0.052390 | 0.007282 | 0.009794 | 0.037819 | 0.054697 | 0.006854 | 0.013643 | 0.035538 | 0.054096 | 0.007189 | 0.011210 |
Golgi | 0.021317 | 0.016687 | 0.001302 | 0.003178 | 0.014143 | 0.018779 | 0.001410 | 0.006616 | 0.015377 | 0.018234 | 0.001325 | 0.004443 |
Lipid Particles | 0.065315 | 0.024559 | 0.003060 | 0.001438 | 0.025367 | 0.019279 | 0.005764 | 0.003030 | 0.032239 | 0.020655 | 0.003649 | 0.002023 |
Mitochondria | 0.010953 | 0.005271 | 0.000251 | 0.000617 | 0.002082 | 0.010085 | 0.000114 | 0.000757 | 0.003608 | 0.008830 | 0.000221 | 0.000669 |
Mitotic Spindle | 0.021281 | 0.171112* | 0.819588* | 0.684782* | 0.080744 | 0.134774* | 0.704277* | 0.643036* | 0.070514 | 0.144245* | 0.794463* | 0.669420* |
None | 0.016500 | 0.005611 | 0.000715 | 0.002897 | 0.005664 | 0.003836 | 0.000768 | 0.000115 | 0.007528 | 0.004298 | 0.000727 | 0.001873 |
Nuclear Periphery | 0.000444 | 0.000340 | 0.000382 | 0.000284 | 0.000218 | 0.000322 | 0.000413 | 0.000217 | 0.000257 | 0.000327 | 0.000389 | 0.000259 |
Nuclear Periphery Foci | 0.040719 | 0.028532 | 0.012582 | 0.006594 | 0.031351 | 0.012329 | 0.022995 | 0.005048 | 0.032963 | 0.016552 | 0.014851 | 0.006025 |
Nucleolus | 0.022786 | 0.006971 | 0.002556 | 0.003107 | 0.009012 | 0.011528 | 0.003404 | 0.000824 | 0.011382 | 0.010340 | 0.002741 | 0.002267 |
Nucleus | 0.029315 | 0.013546 | 0.015531 | 0.006704 | 0.007191 | 0.012065 | 0.007979 | 0.000607 | 0.010997 | 0.012451 | 0.013886 | 0.004461 |
Peroxisomes | 0.097562 | 0.119094* | 0.019039 | 0.022678 | 0.070272 | 0.074536 | 0.022102 | 0.035500 | 0.074967 | 0.086149 | 0.019706 | 0.027396 |
Vacuole | 0.002185 | 0.011497 | 0.008088 | 0.015754 | 0.002741 | 0.013122 | 0.017232 | 0.012064 | 0.002645 | 0.012699 | 0.010080 | 0.014396 |
Vacuole Periphery | 0.000341 | 0.001591 | 0.000211 | 0.000852 | 0.000260 | 0.002264 | 0.000151 | 0.000497 | 0.000274 | 0.002089 | 0.000198 | 0.000721 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | 0.44 | 0.49 | -0.46 | -1.05 | -1.10 | -3.84 | -5.48 | -3.68 | -2.68 | -0.94 | -0.97 | -2.81 | -1.95 | -1.74 | -1.24 |
Bud Neck | 0.31 | -0.13 | -2.28 | -2.69 | -2.47 | -7.86 | -2.27 | -0.31 | 6.99 | 2.01 | -7.11 | -3.03 | -3.08 | 0.32 | -1.81 |
Bud Site | -4.27 | -8.04 | -7.29 | -5.55 | -4.18 | -7.58 | -7.55 | -7.22 | -5.67 | -1.56 | -8.87 | -12.53 | -10.04 | -7.88 | -4.78 |
Cell Periphery | 1.99 | 4.18 | 3.88 | 2.92 | -0.75 | -1.68 | 1.53 | 0.96 | 1.98 | 0.00 | -0.23 | 4.33 | 3.05 | 3.42 | -0.59 |
Cytoplasm | -2.28 | 3.55 | 1.54 | 3.81 | -1.54 | -9.18 | 0.73 | 1.40 | 6.73 | 0.61 | -9.23 | 4.14 | 1.53 | 8.04 | -1.13 |
Cytoplasmic Foci | 6.72 | 28.49 | 20.62 | 16.67 | -3.54 | 7.99 | 38.66 | 17.46 | 13.76 | -3.81 | 10.96 | 65.59 | 32.96 | 25.64 | -5.71 |
Eisosomes | 4.27 | 10.72 | 8.27 | 4.60 | -1.01 | -0.21 | 0.98 | 6.13 | 5.83 | 3.06 | 1.30 | 9.06 | 6.69 | 5.50 | 0.35 |
Endoplasmic Reticulum | 1.02 | -3.41 | -0.02 | -1.08 | 3.44 | -2.15 | -4.01 | -0.69 | 0.40 | 3.44 | -1.44 | -6.94 | -2.53 | -1.92 | 4.67 |
Endosome | -6.57 | 4.09 | 3.57 | 8.60 | 0.59 | -6.91 | 8.14 | 6.06 | 10.45 | 0.37 | -8.56 | 11.67 | 9.05 | 15.28 | 0.53 |
Golgi | 3.20 | 11.30 | 10.29 | 8.82 | -0.71 | -1.89 | 15.67 | 3.96 | 4.73 | -0.88 | -0.54 | 19.76 | 10.45 | 10.37 | -1.15 |
Lipid Particles | 4.57 | 8.25 | 8.52 | 8.80 | 4.03 | 2.21 | 9.42 | 12.08 | 10.33 | 1.02 | 4.16 | 14.70 | 15.64 | 14.52 | 2.93 |
Mitochondria | 0.96 | 2.42 | 2.35 | 3.28 | -1.11 | -5.84 | 4.21 | 1.50 | 6.21 | -0.80 | -4.70 | 3.73 | 3.13 | 7.15 | -1.15 |
Mitotic Spindle | -11.53 | -60.11* | -26.02* | -17.56 | 3.78 | -2.16 | -27.74* | -15.48 | -14.88 | 1.83 | -6.68 | -64.10 | -28.07 | -25.24 | 4.69 |
None | 3.63 | 5.00 | 4.36 | 1.97 | -1.55 | -0.26 | 5.88 | 8.71 | 8.74 | 2.58 | 2.28 | 8.32 | 5.75 | 4.50 | -1.04 |
Nuclear Periphery | 0.21 | -4.11 | -2.79 | -3.39 | 0.43 | -1.85 | -4.74 | -1.97 | -0.82 | 2.70 | -1.54 | -8.02 | -4.25 | -3.53 | 1.65 |
Nuclear Periphery Foci | 4.00 | 5.83 | 7.48 | 4.89 | 3.90 | 11.84 | 4.88 | 9.66 | 2.14 | 3.58 | 11.72 | 10.07 | 13.80 | 4.52 | 5.20 |
Nucleolus | 3.75 | 4.67 | 3.48 | 0.20 | -0.85 | -1.81 | 2.61 | 4.85 | 5.84 | 2.09 | 0.65 | 6.13 | 3.94 | 3.55 | 0.15 |
Nucleus | 1.33 | -0.59 | 0.17 | -1.04 | 0.75 | -5.33 | -2.88 | 2.69 | 7.13 | 3.56 | -3.25 | -5.63 | -1.58 | -0.26 | 2.18 |
Peroxisomes | -2.11 | 9.79 | 9.48 | 13.73 | 0.59 | -2.67 | 9.73 | 4.93 | 6.30 | -1.44 | -4.33 | 16.84 | 11.97 | 15.11 | -0.75 |
Vacuole | -5.06 | -8.58 | -4.58 | -3.06 | -2.27 | -7.69 | -4.62 | -3.33 | -2.48 | -1.11 | -9.19 | -9.69 | -5.65 | -4.10 | -2.61 |
Vacuole Periphery | -2.81 | -0.55 | -1.21 | 1.01 | -1.13 | -3.73 | -1.77 | -1.47 | 2.95 | -0.98 | -4.36 | -2.39 | -1.70 | 2.52 | -1.33 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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