Standard name | |
---|---|
Human Ortholog | |
Description | Nucleoside monophosphate and nucleoside diphosphate kinase; functions in the de novo biosynthesis of pyrimidine deoxyribonucleotides; thymidylate/uridylate kinase that converts nucleoside monophosphates, dTMP and dUMP to nucleoside diphosphates, dTDP and dUDP; nucleoside diphosphate kinase that converts nucleoside diphosphates, dTDP and dUDP to dTTP and dUTP; essential for mitotic and meiotic DNA replication; homologous to S. pombe tmp1; human homolog DTYMK can complement the cdc8 null mutant |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 1 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 1 | 1 | 1 | 1 | 1 |
Bud | 0 | 0 | 0 | 0 | 0 | 3 | 2 | – | 6 | 3 | 4 | 13 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 3 | 0 | 8 | 4 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 1 | 2 | 7 | 1 | 2 | 1 | 7 | – | 4 | 4 | 5 | 9 | 0 | 1 | 2 | 6 | 6 | 4 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 169 | 237 | 422 | 277 | 335 | 310 | 429 | – | 430 | 429 | 374 | 422 | 181 | 369 | 347 | 120 | 145 | 175 | 167 | 226 | 405 | 211 | 350 | 391 |
Endoplasmic Reticulum | 3 | 2 | 1 | 0 | 0 | 1 | 1 | – | 1 | 1 | 0 | 1 | 1 | 1 | 0 | 5 | 5 | 9 | 1 | 0 | 0 | 1 | 7 | 2 |
Endosome | 0 | 0 | 1 | 0 | 0 | 0 | 3 | – | 0 | 2 | 4 | 1 | 0 | 0 | 1 | 0 | 0 | 2 | 4 | 2 | 1 | 1 | 1 | 4 |
Golgi | 3 | 2 | 0 | 1 | 0 | 0 | 1 | – | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 2 | 3 | 3 | 1 | 0 | 2 | 0 |
Mitochondria | 2 | 1 | 0 | 6 | 7 | 66 | 29 | – | 155 | 110 | 158 | 183 | 1 | 1 | 0 | 1 | 2 | 5 | 1 | 2 | 3 | 0 | 2 | 2 |
Nucleus | 1 | 2 | 1 | 1 | 1 | 4 | 11 | – | 13 | 18 | 5 | 14 | 1 | 1 | 0 | 9 | 2 | 1 | 0 | 1 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 1 | 0 | 0 | 0 | 0 | 0 | – | 2 | 2 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 1 | – | 2 | 1 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 2 | – | 1 | 2 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 4 | 2 |
Vac/Vac Membrane | 2 | 4 | 6 | 7 | 27 | 11 | 50 | – | 56 | 57 | 38 | 31 | 1 | 2 | 5 | 5 | 6 | 5 | 7 | 9 | 0 | 1 | 1 | 0 |
Unique Cell Count | 178 | 247 | 428 | 283 | 349 | 343 | 483 | 562 | 550 | 503 | 588 | 184 | 372 | 350 | 128 | 150 | 182 | 193 | 253 | 424 | 222 | 389 | 418 | |
Labelled Cell Count | 181 | 251 | 438 | 293 | 372 | 396 | 536 | 671 | 630 | 593 | 679 | 185 | 375 | 356 | 146 | 166 | 203 | 193 | 253 | 424 | 222 | 389 | 418 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 6.3 | 6.6 | 6.5 | 6.1 | 6.8 | 4.9 | 5.7 | – | 5.0 | 5.3 | 5.0 | 5.0 | 6.7 | 6.9 | 7.2 | 8.2 | 8.3 | 8.4 | 5.9 | 6.4 | 6.3 |
Std Deviation (1e-4) | 0.9 | 1.1 | 1.1 | 1.6 | 1.5 | 1.5 | 1.8 | – | 1.4 | 1.2 | 1.4 | 0.9 | 1.0 | 1.2 | 1.7 | 1.1 | 1.2 | 1.5 | 1.1 | 2.1 | 1.8 |
Intensity Change (Log2) | – | – | – | -0.09 | 0.07 | -0.4 | -0.18 | – | -0.38 | -0.3 | -0.36 | -0.38 | 0.05 | 0.09 | 0.16 | 0.33 | 0.36 | 0.37 | -0.14 | -0.02 | -0.04 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 3.1 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | -0.2 | – | 0 | 0 | -0.9 | -0.1 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | -0.7 | -2.3 | -5.2 | -5.9 | – | -9.9 | -9.5 | -10.5 | -11.3 | -0.2 | 0.8 | 0.7 | -3.1 | -1.5 | -1.9 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 9.5 | 5.2 | – | 11.8 | 9.8 | 12.7 | 12.7 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 2.7 | – | 2.7 | 3.4 | 0 | 2.8 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 1.0 | 4.4 | 1.7 | 5.6 | – | 5.5 | 5.7 | 4.4 | 3.3 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 1.2965 | 1.9084 | 1.7565 | 1.5865 | 1.2326 | 1.3622 | 2.8255 | 3.532 | 3.5134 | 2.7865 | 2.8825 | 3.1189 | 4.8785 | 6.2656 | 5.8207 | 5.8546 | 6.1073 | 5.8163 |
Actin | 0.0286 | 0.0023 | 0.011 | 0.0148 | 0.0389 | 0.0128 | 0.06 | 0.001 | 0.0161 | 0.0053 | 0.0212 | 0.0052 | 0.0221 | 0.0004 | 0.0201 | 0.0008 | 0.0014 | 0.0004 |
Bud | 0.0008 | 0.0041 | 0.0005 | 0.0352 | 0.0007 | 0.0002 | 0.0014 | 0.0012 | 0.0015 | 0.0023 | 0.0151 | 0.0005 | 0.0001 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0 |
Bud Neck | 0.0008 | 0.0001 | 0.0002 | 0.0005 | 0.0008 | 0.0024 | 0.0067 | 0.0002 | 0.0007 | 0.0005 | 0.0005 | 0.0011 | 0.0005 | 0.0001 | 0.0032 | 0.0003 | 0.0004 | 0.0005 |
Bud Periphery | 0.0008 | 0.0016 | 0.0003 | 0.0154 | 0.001 | 0.0002 | 0.0018 | 0.0006 | 0.0014 | 0.0027 | 0.0191 | 0.0005 | 0.0001 | 0 | 0.0003 | 0 | 0.0001 | 0 |
Bud Site | 0.0028 | 0.0062 | 0.0009 | 0.0028 | 0.0045 | 0.0001 | 0.0151 | 0.0019 | 0.006 | 0.002 | 0.0019 | 0.0009 | 0.0003 | 0.0003 | 0.001 | 0.0002 | 0.0004 | 0 |
Cell Periphery | 0.0001 | 0.0001 | 0.0001 | 0.0005 | 0.0001 | 0 | 0.0004 | 0.0001 | 0.0002 | 0.0001 | 0.0003 | 0 | 0.0001 | 0 | 0.0002 | 0.0001 | 0 | 0 |
Cytoplasm | 0.7676 | 0.8323 | 0.9107 | 0.7429 | 0.7961 | 0.8645 | 0.6571 | 0.9103 | 0.8075 | 0.752 | 0.639 | 0.8836 | 0.8262 | 0.8593 | 0.867 | 0.8753 | 0.7589 | 0.8936 |
Cytoplasmic Foci | 0.0171 | 0.0129 | 0.0082 | 0.0124 | 0.0214 | 0.0086 | 0.0255 | 0.0088 | 0.0103 | 0.028 | 0.0151 | 0.0131 | 0.0028 | 0.004 | 0.0032 | 0.0065 | 0.0266 | 0.0033 |
Eisosomes | 0.0001 | 0 | 0 | 0.0001 | 0.0002 | 0.0001 | 0.0004 | 0 | 0.0002 | 0 | 0.0002 | 0.0001 | 0.0002 | 0 | 0.0002 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0.0087 | 0.003 | 0.0053 | 0.0036 | 0.0076 | 0.0028 | 0.0071 | 0.0013 | 0.0035 | 0.002 | 0.0044 | 0.0038 | 0.0098 | 0.0029 | 0.0028 | 0.0057 | 0.0046 | 0.0029 |
Endosome | 0.0264 | 0.0041 | 0.0039 | 0.0117 | 0.0263 | 0.012 | 0.0305 | 0.0026 | 0.0123 | 0.0044 | 0.0329 | 0.0079 | 0.0079 | 0.0014 | 0.0034 | 0.0021 | 0.012 | 0.0013 |
Golgi | 0.0033 | 0.0001 | 0.0014 | 0.01 | 0.0099 | 0.0033 | 0.0088 | 0.0008 | 0.0052 | 0.0047 | 0.0232 | 0.0042 | 0.0012 | 0.0001 | 0.0024 | 0.0001 | 0.0016 | 0.0001 |
Lipid Particles | 0.0018 | 0.0004 | 0.0014 | 0.0006 | 0.0051 | 0.0004 | 0.0162 | 0 | 0.0027 | 0.0022 | 0.0292 | 0.0038 | 0.0004 | 0.0001 | 0.0006 | 0 | 0.0062 | 0 |
Mitochondria | 0.0021 | 0.0002 | 0.0004 | 0.0135 | 0.0046 | 0.0059 | 0.0053 | 0.0006 | 0.0053 | 0.0008 | 0.0182 | 0.0064 | 0.0004 | 0.0001 | 0.0014 | 0.0001 | 0.0003 | 0.0001 |
None | 0.0942 | 0.1225 | 0.0466 | 0.1182 | 0.0443 | 0.0741 | 0.1233 | 0.0627 | 0.1046 | 0.1577 | 0.1566 | 0.0456 | 0.1123 | 0.1265 | 0.0883 | 0.0997 | 0.1299 | 0.0878 |
Nuclear Periphery | 0.0132 | 0.0017 | 0.0021 | 0.0047 | 0.015 | 0.0019 | 0.0117 | 0.0009 | 0.0031 | 0.0012 | 0.0044 | 0.0035 | 0.0042 | 0.001 | 0.001 | 0.0019 | 0.0022 | 0.001 |
Nucleolus | 0.0005 | 0.0002 | 0.0001 | 0.0002 | 0.0003 | 0.0001 | 0.0007 | 0 | 0.0004 | 0.0001 | 0.0002 | 0.0001 | 0 | 0 | 0.0001 | 0 | 0.0008 | 0 |
Nucleus | 0.017 | 0.0034 | 0.0034 | 0.0061 | 0.0044 | 0.0076 | 0.0057 | 0.0032 | 0.007 | 0.0033 | 0.0049 | 0.0088 | 0.0096 | 0.0024 | 0.0029 | 0.0043 | 0.0026 | 0.0073 |
Peroxisomes | 0.0023 | 0.0001 | 0.0011 | 0.0027 | 0.0122 | 0.0002 | 0.011 | 0.002 | 0.0047 | 0.0256 | 0.0074 | 0.0066 | 0.0001 | 0.0001 | 0.0003 | 0.0002 | 0.0027 | 0.0001 |
Punctate Nuclear | 0.0063 | 0.0012 | 0.0012 | 0.0008 | 0.0027 | 0.0014 | 0.0068 | 0.0006 | 0.0059 | 0.0039 | 0.0037 | 0.0029 | 0.0006 | 0.0006 | 0.0004 | 0.0016 | 0.048 | 0.0012 |
Vacuole | 0.0042 | 0.0033 | 0.0011 | 0.0025 | 0.0021 | 0.0012 | 0.0029 | 0.0009 | 0.0012 | 0.0009 | 0.0015 | 0.0008 | 0.0007 | 0.0005 | 0.0007 | 0.0008 | 0.0011 | 0.0003 |
Vacuole Periphery | 0.0015 | 0.0002 | 0.0002 | 0.001 | 0.0019 | 0.0004 | 0.0015 | 0.0002 | 0.0004 | 0.0002 | 0.0009 | 0.0007 | 0.0003 | 0.0001 | 0.0003 | 0.0001 | 0.0002 | 0.0001 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 40.6785 | 31.8186 | 7.8703 | 22.5367 | 42.8524 | 42.35 | 25.2778 | 17.0608 | 20.24 | 34.8731 |
Translational Efficiency | 1.3119 | 0.9155 | 2.7194 | 1.5699 | 0.8261 | 1.3089 | 1.168 | 1.4929 | 1.602 | 1.2751 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
276 | 1422 | 105 | 1969 | 1378 | 2483 | 2477 | 1901 | 1654 | 3905 | 2582 | 3870 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 684.08 | 768.07 | 963.68 | 845.00 | 680.74 | 803.31 | 922.71 | 914.06 | 681.30 | 790.48 | 924.38 | 878.92 |
Standard Deviation | 91.31 | 110.78 | 108.83 | 126.93 | 80.60 | 105.36 | 111.77 | 117.76 | 82.49 | 108.70 | 111.94 | 127.28 |
Intensity Change Log 2 | — | 0.167073 | 0.494389 | 0.304786 | — | 0.238853 | 0.438773 | 0.425185 | — | 0.203321 | 0.466917 | 0.366094 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000111 | 0.000561 | 0.000730 | 0.000416 | 0.000072 | 0.000317 | 0.000095 | 0.000230 | 0.000078 | 0.000406 | 0.000121 | 0.000324 |
Bud Neck | 0.031270 | 0.005457 | 0.001593 | 0.006775 | 0.006583 | 0.015721 | 0.006431 | 0.006226 | 0.010702 | 0.011984 | 0.006235 | 0.006505 |
Bud Site | 0.016724 | 0.006994 | 0.007028 | 0.025289 | 0.003350 | 0.014583 | 0.010763 | 0.021274 | 0.005582 | 0.011820 | 0.010611 | 0.023317 |
Cell Periphery | 0.000274 | 0.000114 | 0.000301 | 0.000131 | 0.000101 | 0.000114 | 0.000036 | 0.000190 | 0.000130 | 0.000114 | 0.000047 | 0.000160 |
Cytoplasm | 0.455928* | 0.647635* | 0.387114* | 0.638194* | 0.594302* | 0.618016* | 0.726225* | 0.596386* | 0.571212* | 0.628801* | 0.712435* | 0.617657* |
Cytoplasmic Foci | 0.199457* | 0.134127 | 0.021985 | 0.015935 | 0.152905 | 0.097953 | 0.006110 | 0.016484 | 0.160673 | 0.111126 | 0.006756 | 0.016205 |
Eisosomes | 0.000149 | 0.000042 | 0.000102 | 0.000029 | 0.000060 | 0.000043 | 0.000008 | 0.000054 | 0.000075 | 0.000042 | 0.000012 | 0.000041 |
Endoplasmic Reticulum | 0.001538 | 0.002171 | 0.007003 | 0.001165 | 0.000905 | 0.000963 | 0.000357 | 0.001189 | 0.001011 | 0.001403 | 0.000628 | 0.001177 |
Endosome | 0.013592 | 0.008669 | 0.006725 | 0.003456 | 0.003784 | 0.008944 | 0.001010 | 0.002246 | 0.005421 | 0.008844 | 0.001242 | 0.002862 |
Golgi | 0.001461 | 0.002358 | 0.000151 | 0.001829 | 0.001275 | 0.001940 | 0.000292 | 0.000810 | 0.001306 | 0.002092 | 0.000287 | 0.001328 |
Lipid Particles | 0.007373 | 0.003647 | 0.003712 | 0.000758 | 0.004338 | 0.002622 | 0.000241 | 0.000819 | 0.004845 | 0.002995 | 0.000383 | 0.000788 |
Mitochondria | 0.002079 | 0.002187 | 0.000359 | 0.001141 | 0.001446 | 0.004621 | 0.001391 | 0.002083 | 0.001551 | 0.003734 | 0.001349 | 0.001603 |
Mitotic Spindle | 0.000359 | 0.001707 | 0.000262 | 0.025349 | 0.001490 | 0.009174 | 0.021322 | 0.012748 | 0.001301 | 0.006455 | 0.020466 | 0.019159 |
None | 0.004541 | 0.007640 | 0.002854 | 0.006880 | 0.005096 | 0.004438 | 0.004776 | 0.005464 | 0.005004 | 0.005604 | 0.004698 | 0.006185 |
Nuclear Periphery | 0.000710 | 0.000429 | 0.001811 | 0.000859 | 0.000288 | 0.000483 | 0.000695 | 0.000695 | 0.000359 | 0.000463 | 0.000741 | 0.000779 |
Nuclear Periphery Foci | 0.000616 | 0.001009 | 0.003754 | 0.001131 | 0.000338 | 0.000223 | 0.000154 | 0.000552 | 0.000384 | 0.000510 | 0.000300 | 0.000847 |
Nucleolus | 0.001309 | 0.001046 | 0.000614 | 0.000697 | 0.000726 | 0.001179 | 0.000284 | 0.001183 | 0.000823 | 0.001130 | 0.000298 | 0.000936 |
Nucleus | 0.206384* | 0.118699 | 0.416430* | 0.164975* | 0.190583* | 0.141574* | 0.175176* | 0.241555* | 0.193220* | 0.133244* | 0.184987* | 0.202593* |
Peroxisomes | 0.004351 | 0.002901 | 0.000416 | 0.000547 | 0.001873 | 0.002988 | 0.000177 | 0.000790 | 0.002287 | 0.002956 | 0.000186 | 0.000666 |
Vacuole | 0.050480 | 0.050911 | 0.136803 | 0.101423 | 0.029915 | 0.072404 | 0.043946 | 0.088191 | 0.033347 | 0.064577 | 0.047722 | 0.094923 |
Vacuole Periphery | 0.001295 | 0.001697 | 0.000255 | 0.003020 | 0.000571 | 0.001701 | 0.000509 | 0.000832 | 0.000692 | 0.001699 | 0.000499 | 0.001945 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -1.28 | -5.67 | -2.33 | 0.71 | 3.60 | -2.94 | -1.93 | -5.29 | 1.16 | -3.28 | -2.37 | -3.08 | -4.17 | 0.98 | -2.54 |
Bud Neck | 3.80 | 4.27 | 3.84 | 0.49 | -5.14 | -7.03 | 0.71 | 0.55 | 9.25 | -0.28 | -0.87 | 3.37 | 3.62 | 9.13 | 0.13 |
Bud Site | 1.91 | 1.79 | 0.31 | -4.55 | -2.71 | -7.48 | -3.94 | -5.71 | 0.32 | -2.86 | -4.59 | -1.80 | -5.61 | -1.83 | -4.12 |
Cell Periphery | 3.36 | -0.43 | 3.86 | 2.18 | 6.08 | -0.46 | 5.25 | -1.32 | -0.42 | -8.70 | 0.89 | 6.34 | 1.60 | 0.41 | -10.30 |
Cytoplasm | -10.70 | 5.02 | -5.61 | 9.94 | -9.94 | -2.82 | -6.39 | 8.42 | 12.27 | 16.24 | -7.59 | -7.74 | 4.52 | 15.10 | 14.42 |
Cytoplasmic Foci | 5.24 | 15.22 | 16.09 | 26.74 | 1.75 | 10.32 | 31.48 | 29.90 | 30.67 | -8.28 | 10.09 | 35.34 | 33.79 | 40.42 | -10.86 |
Eisosomes | 5.84 | 1.23 | 6.49 | 4.01 | 7.58 | 5.88 | 18.53 | 5.59 | -0.17 | -17.51 | 8.10 | 15.30 | 9.66 | 3.14 | -16.71 |
Endoplasmic Reticulum | -1.18 | -6.94 | 0.77 | 2.78 | 7.58 | -0.21 | 1.23 | -1.64 | -1.92 | -4.36 | -1.55 | 0.17 | -1.37 | 0.38 | -2.85 |
Endosome | 1.33 | 1.73 | 3.21 | 8.21 | 5.35 | -7.44 | 8.26 | 5.97 | 12.41 | -3.63 | -4.09 | 6.62 | 5.39 | 14.57 | -3.94 |
Golgi | -1.68 | 3.38 | 1.13 | 2.87 | -1.85 | -1.38 | 4.05 | 2.69 | 3.50 | -1.07 | -2.13 | 4.75 | 2.18 | 4.11 | -2.00 |
Lipid Particles | 2.55 | 2.06 | 4.59 | 7.69 | 6.52 | 2.21 | 5.32 | 4.68 | 4.81 | -8.59 | 2.70 | 6.47 | 6.00 | 8.01 | -6.10 |
Mitochondria | -0.14 | 5.51 | 4.09 | 2.41 | -4.91 | -5.13 | 0.99 | 0.32 | 4.84 | -0.38 | -4.75 | 1.59 | 1.98 | 5.80 | 0.24 |
Mitotic Spindle | -2.29 | -0.19 | -8.98 | -8.21 | -8.98 | -4.82 | -7.48 | -5.74 | -1.10 | 2.02 | -4.61 | -7.68 | -9.69 | -5.67 | 0.06 |
None | -0.78 | 0.94 | -0.17 | 1.42 | -4.08 | 0.84 | 0.15 | -0.86 | -2.97 | -2.39 | -0.45 | 0.29 | -0.75 | -0.36 | -2.65 |
Nuclear Periphery | 1.87 | -7.16 | -4.13 | -9.83 | 5.11 | -2.59 | -19.58 | -19.81 | -10.38 | -2.67 | -1.79 | -17.69 | -19.71 | -13.55 | -1.61 |
Nuclear Periphery Foci | -1.71 | -6.46 | -1.82 | -0.08 | 5.77 | 3.08 | 2.79 | -5.42 | -8.10 | -7.84 | -1.77 | 0.04 | -5.35 | -3.65 | -5.73 |
Nucleolus | 0.63 | 2.14 | 2.18 | 3.89 | 0.15 | -2.39 | 3.89 | -0.33 | 1.12 | -2.24 | -1.85 | 4.61 | 1.15 | 3.01 | -2.70 |
Nucleus | 6.63 | -7.37 | 0.67 | -13.65 | 8.41 | 8.52 | -6.10 | -11.61 | -21.73 | -6.66 | 11.60 | -7.10 | -7.33 | -24.24 | 0.34 |
Peroxisomes | 1.12 | 3.36 | 3.44 | 5.34 | 1.05 | -1.49 | 3.86 | 3.20 | 6.24 | -2.64 | -0.97 | 4.99 | 4.46 | 8.26 | -3.43 |
Vacuole | 0.11 | -6.77 | -16.12 | -22.42 | 1.29 | -15.03 | -15.00 | -25.08 | -14.89 | -13.51 | -13.28 | -14.94 | -34.50 | -25.50 | -18.05 |
Vacuole Periphery | -1.32 | 4.80 | -0.25 | 0.58 | -2.74 | -6.10 | 1.51 | 0.79 | 6.25 | -0.64 | -6.55 | 2.41 | -1.01 | 2.95 | -2.40 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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