Standard name
Human Ortholog
Description Methylene-fatty-acyl-phospholipid synthase; catalyzes the last two steps in phosphatidylcholine biosynthesis; also known as phospholipid methyltransferase
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0.06 0.06 0 0 0 0 0 0 0 0 0
Cytoplasm 0.46 0.47 0.37 0.37 0.55 0.57 0.59 0.66 0.67 0.66 0.7 0.67 0.19 0.26 0.23 0.33 0.26 0.32 0.05 0 0 0 0 0
Endoplasmic Reticulum 0.34 0.49 0.5 0.6 0.4 0.37 0.31 0.28 0.24 0.24 0.23 0.22 0.72 0.68 0.66 0 0 0 0.6 0.7 0.69 0.78 0.74 0.66
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.3 0.1 0.19 0 0.06 0 0.1 0.07 0.09 0.11 0.1 0.12 0.12 0.06 0.12 0.64 0.71 0.64 0.31 0.24 0.25 0.17 0.2 0.26
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 1 1 1 5 5 1 0 4 6 2 3 1 3 1 3 0 0 1 0 1 0 0 0 0
Bud 1 1 1 2 2 6 4 3 2 2 2 4 0 1 1 0 0 0 0 1 0 2 2 3
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 2 3 2 9 7 9 11 10 8 10 8 12 2 8 9 1 1 2 0 0 0 0 0 0
Cytoplasm 116 108 52 65 117 131 174 238 199 188 153 178 24 37 36 35 32 33 15 8 3 2 2 2
Endoplasmic Reticulum 87 113 70 105 84 84 91 100 70 69 50 58 92 95 103 1 1 1 176 196 117 166 176 179
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 1
Golgi 1 0 0 2 2 0 0 0 0 1 0 0 0 4 1 3 3 4 0 0 0 0 1 2
Mitochondria 0 0 0 1 0 2 3 0 1 2 0 2 0 0 0 0 0 0 1 0 0 0 1 0
Nucleus 1 0 0 0 0 0 0 1 0 0 0 1 0 1 0 3 2 6 0 0 0 0 0 1
Nuclear Periphery 0 0 1 2 0 1 1 0 1 0 1 0 0 0 0 0 1 0 7 2 5 2 2 5
Nucleolus 0 0 0 1 0 1 1 1 0 0 0 1 0 0 0 0 0 1 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 77 24 26 7 12 9 29 25 26 32 21 31 16 8 19 69 89 65 91 66 43 36 48 71
Unique Cell Count 253 231 140 176 212 228 293 362 295 286 219 267 128 140 155 107 125 102 296 280 171 214 239 271
Labelled Cell Count 286 250 153 199 229 244 314 382 313 306 238 288 137 155 172 112 129 113 296 280 171 214 239 271


Endoplasmic Reticulum, Vacuole

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 31.8 38.3 21.4 28.3 34.0 31.8 29.8 32.5 31.0 30.1 31.2 29.7 34.2 28.8 26.9 40.8 42.2 45.2 37.3 40.1 38.6
Std Deviation (1e-4) 7.0 9.7 5.7 7.9 9.8 8.7 8.5 7.9 9.2 8.4 9.8 8.7 7.7 8.0 7.2 12.2 12.3 13.3 10.3 12.7 12.1
Intensity Change (Log2) – – – 0.4 0.66 0.57 0.48 0.6 0.53 0.49 0.54 0.47 0.67 0.43 0.33 0.93 0.98 1.07 0.8 0.9 0.85


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 1.9 2.0 0 0 0
Cytoplasm 0 3.3 3.8 4.3 5.8 6.0 5.6 6.1 5.7 -3.3 -1.9 -2.6 -0.7 -2.0 -0.8
Endoplasmic Reticulum 1.7 -1.9 -2.5 -3.8 -4.8 -5.5 -5.4 -5.3 -5.8 3.7 3.0 2.9 -8.4 -9.0 -8.3
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole -4.2 -3.8 -4.6 -2.5 -3.9 -2.9 -2.1 -2.5 -1.9 -1.4 -3.3 -1.5 7.3 8.6 7.2

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 32.2212 38.7672 33.5083 28.3789 32.486 35.2274 39.2674 50.1015 43.6663 40.7836 41.9466 45.3281 37.921 45.4359 38.0212 36.6986 34.1921 41.2838
Actin 0.0082 0.0001 0.0109 0.0003 0.0059 0.0017 0.0078 0 0.0096 0.0001 0.0041 0 0.0001 0 0.0001 0.0001 0 0.0001
Bud 0.0003 0.0001 0.0006 0.0006 0.0006 0.0003 0.0002 0 0.0001 0.0003 0.0003 0.0001 0.0001 0.0001 0.0001 0.0003 0.0001 0.0002
Bud Neck 0.0007 0.0003 0.0007 0.001 0.001 0.0008 0.0003 0.0003 0.0006 0.0014 0.0007 0.0005 0.0003 0.0003 0.0008 0.0006 0.0005 0.0008
Bud Periphery 0.0014 0.0008 0.0016 0.0025 0.0037 0.0018 0.001 0.0005 0.001 0.0023 0.0013 0.0011 0.0007 0.0006 0.0014 0.0021 0.0011 0.0017
Bud Site 0.0014 0.0001 0.0025 0.0031 0.0006 0.0002 0.0002 0 0.0003 0.0002 0.0004 0 0.0001 0 0.0001 0.0003 0.0001 0.0001
Cell Periphery 0.1249 0.17 0.1071 0.1261 0.1037 0.108 0.0796 0.124 0.1267 0.1333 0.1127 0.0691 0.071 0.1284 0.1049 0.0817 0.0807 0.0651
Cytoplasm 0.0093 0.0061 0.0143 0.0092 0.0074 0.0037 0.0098 0.0055 0.0092 0.0072 0.0099 0.0042 0.0063 0.0038 0.0062 0.0049 0.0041 0.0038
Cytoplasmic Foci 0.003 0.0002 0.0092 0.0113 0.008 0.0015 0.0062 0 0.0024 0.0001 0.014 0 0.0001 0 0.0001 0.0001 0.0001 0.0001
Eisosomes 0.0002 0 0.0005 0.0002 0 0 0.0001 0 0.0002 0 0 0 0 0 0 0 0 0
Endoplasmic Reticulum 0.255 0.211 0.2683 0.0677 0.0468 0.1333 0.3661 0.4339 0.2922 0.2009 0.1659 0.2651 0.4014 0.4361 0.407 0.3058 0.2586 0.4507
Endosome 0.0076 0.0016 0.0135 0.0094 0.0056 0.0037 0.0082 0.0009 0.0043 0.0027 0.0097 0.002 0.0027 0.0006 0.0023 0.0103 0.0029 0.0032
Golgi 0.0017 0.0001 0.0037 0.0023 0.003 0.0004 0.0007 0 0.0006 0.0001 0.0017 0.0001 0.0002 0 0.0001 0.0004 0.0001 0.0002
Lipid Particles 0.007 0.0045 0.0101 0.0081 0.0255 0.0057 0.0083 0.0033 0.0117 0.0035 0.0083 0.0029 0.0066 0.0039 0.0033 0.0021 0.0058 0.0081
Mitochondria 0.0082 0.0001 0.002 0.0103 0.0018 0.002 0.0019 0.0001 0.0012 0.0003 0.0004 0.0001 0.0008 0 0.0002 0.0007 0.0002 0.0003
None 0.0006 0 0.0079 0.0042 0.0005 0.0011 0.0045 0 0.003 0.0001 0.0024 0 0.0001 0 0 0.0001 0 0
Nuclear Periphery 0.0957 0.0458 0.0461 0.0303 0.0408 0.0713 0.13 0.0543 0.0626 0.0884 0.1067 0.1121 0.2201 0.0767 0.0787 0.0793 0.087 0.1252
Nucleolus 0.0034 0.0017 0.0038 0.0082 0.0013 0.0057 0.0091 0.0002 0.0026 0.0009 0.002 0.0004 0.0052 0.0001 0.0004 0.0049 0.0012 0.0006
Nucleus 0.0096 0.0036 0.0079 0.0094 0.0061 0.009 0.0119 0.0032 0.0045 0.0065 0.0142 0.0037 0.0122 0.0026 0.0032 0.0095 0.0046 0.0077
Peroxisomes 0.0005 0 0.0039 0.0011 0.046 0.0001 0.0004 0 0.0011 0 0.0031 0 0 0 0 0 0 0
Punctate Nuclear 0.0006 0.0001 0.0094 0.0006 0.0046 0.001 0.0035 0 0.0008 0 0.0062 0 0.0002 0 0 0 0 0.0001
Vacuole 0.4168 0.5292 0.4449 0.6471 0.6192 0.592 0.3073 0.3579 0.4263 0.5015 0.4875 0.4777 0.2421 0.3269 0.3637 0.4336 0.4637 0.2975
Vacuole Periphery 0.0442 0.0247 0.031 0.0467 0.068 0.0567 0.0428 0.0159 0.039 0.0503 0.0487 0.061 0.0296 0.0197 0.0274 0.0634 0.0891 0.0347

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 163.4673 127.0694 90.7551 85.4827 85.2378 159.8252 111.6168 103.5162 139.4716 106.8394
Translational Efficiency 1.2524 1.1493 0.9656 1.3972 0.9821 1.5155 0.991 1.0841 0.9215 0.8619

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✔
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Methylene-fatty-acyl-phospholipid synthase; catalyzes the last two steps in phosphatidylcholine biosynthesis; also known as phospholipid methyltransferase
Localization
Cell Percentages ER (88%)
Cell Cycle Regulation No
Subcompartmental Group ER-5

Opi3

Opi3


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Opi3-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available