Standard name | |
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Human Ortholog | |
Description | tRNA threonylcarbamoyladenosine dehydratase; required for the ct6A tRNA base modification, where an adenosine at position 37 is modified to form a cyclized active ester with an oxazolone ring; localized to the mitochondrial outer membrane; TCD2 has a paralog, TCD1, that arose from the whole genome duplication |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 2 | 1 | 1 | 0 | 0 | 0 | 1 | 1 | 0 | 1 | 1 | 0 | 2 | 0 | 2 | 3 | 1 | 2 | 4 | 1 | 3 | 2 | 9 | 8 |
Bud | 1 | 1 | 0 | 7 | 2 | 4 | 9 | 5 | 11 | 9 | 9 | 10 | 1 | 1 | 2 | 1 | 3 | 0 | 0 | 0 | 0 | 1 | 2 | 4 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 1 |
Bud Site | 1 | 1 | 1 | 0 | 2 | 3 | 3 | 3 | 1 | 7 | 2 | 2 | 0 | 0 | 0 | 0 | 0 | 1 | – | – | – | – | – | – |
Cell Periphery | 4 | 4 | 10 | 2 | 1 | 3 | 3 | 1 | 1 | 2 | 2 | 3 | 7 | 20 | 12 | 4 | 6 | 2 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0 | 0 | 5 | 12 | 21 | 11 | 13 | 10 | 16 | 12 | 7 | 2 | 5 | 10 | 5 | 4 | 6 | 8 | 0 | 0 | 0 | 0 | 1 | 0 |
Endoplasmic Reticulum | 2 | 1 | 0 | 0 | 2 | 0 | 5 | 4 | 2 | 3 | 3 | 3 | 3 | 0 | 4 | 2 | 5 | 2 | 1 | 0 | 0 | 1 | 1 | 2 |
Endosome | 1 | 0 | 1 | 0 | 4 | 1 | 2 | 9 | 4 | 1 | 3 | 7 | 0 | 6 | 3 | 2 | 2 | 0 | 4 | 2 | 1 | 4 | 6 | 4 |
Golgi | 4 | 3 | 10 | 2 | 7 | 1 | 0 | 3 | 0 | 0 | 0 | 0 | 51 | 155 | 208 | 13 | 21 | 6 | 17 | 13 | 13 | 26 | 44 | 32 |
Mitochondria | 315 | 118 | 247 | 155 | 238 | 264 | 369 | 378 | 373 | 560 | 351 | 542 | 183 | 224 | 243 | 6 | 10 | 1 | 286 | 98 | 239 | 157 | 251 | 227 |
Nucleus | 0 | 1 | 0 | 0 | 1 | 1 | 3 | 4 | 6 | 2 | 1 | 4 | 1 | 1 | 0 | 0 | 3 | 1 | 0 | 0 | 0 | 1 | 3 | 0 |
Nuclear Periphery | 19 | 3 | 4 | 4 | 9 | 28 | 48 | 56 | 41 | 60 | 28 | 57 | 7 | 2 | 2 | 3 | 6 | 1 | 2 | 0 | 0 | 0 | 2 | 1 |
Nucleolus | 31 | 8 | 9 | 3 | 22 | 45 | 62 | 52 | 39 | 55 | 29 | 43 | 4 | 2 | 0 | 3 | 6 | 2 | 0 | 1 | 0 | 0 | 1 | 2 |
Peroxisomes | 0 | 0 | 9 | 1 | 5 | 1 | 4 | 0 | 4 | 2 | 3 | 4 | 4 | 3 | 2 | 2 | 6 | 4 | 4 | 0 | 19 | 2 | 6 | 4 |
SpindlePole | 0 | 1 | 0 | 0 | 1 | 0 | 2 | 4 | 1 | 6 | 1 | 5 | 1 | 0 | 0 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 |
Vac/Vac Membrane | 34 | 16 | 19 | 1 | 18 | 10 | 11 | 19 | 21 | 36 | 19 | 45 | 37 | 98 | 67 | 12 | 31 | 14 | 6 | 7 | 2 | 18 | 36 | 24 |
Unique Cell Count | 330 | 126 | 269 | 172 | 281 | 289 | 416 | 432 | 425 | 619 | 378 | 580 | 223 | 357 | 345 | 43 | 96 | 42 | 333 | 129 | 283 | 220 | 376 | 317 |
Labelled Cell Count | 414 | 158 | 316 | 187 | 333 | 372 | 535 | 549 | 520 | 756 | 459 | 727 | 306 | 522 | 550 | 56 | 108 | 45 | 333 | 129 | 283 | 220 | 376 | 317 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 11.0 | 13.9 | 12.3 | 10.8 | 10.0 | 8.5 | 8.2 | 8.3 | 8.1 | 7.8 | 7.3 | 7.5 | 12.9 | 13.0 | 12.1 | 12.0 | 11.7 | 11.2 | 13.7 | 13.4 | 14.9 |
Std Deviation (1e-4) | 1.9 | 3.3 | 2.8 | 2.7 | 2.4 | 2.6 | 2.1 | 2.5 | 2.4 | 2.3 | 2.0 | 1.9 | 2.5 | 2.2 | 2.1 | 3.3 | 3.0 | 3.8 | 2.8 | 3.2 | 3.0 |
Intensity Change (Log2) | – | – | – | -0.19 | -0.3 | -0.53 | -0.59 | -0.56 | -0.61 | -0.66 | -0.75 | -0.72 | 0.07 | 0.08 | -0.02 | -0.04 | -0.07 | -0.13 | 0.16 | 0.12 | 0.27 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | -2.8 | -2.1 | -2.8 | 0 | 0 | 0 | 0 | 0 | -0.3 | 1.1 | -0.2 | 0 | 0 | 0 |
Cytoplasm | 2.7 | 3.1 | 1.4 | 1.0 | 0.4 | 1.4 | 0.1 | 0 | 0 | 0 | 0.8 | 0 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | -0.8 | -2.9 | 0 | 0 | 0 | 0 | 0 | 0 | 6.4 | 11.2 | 14.5 | 0 | 5.5 | 0 |
Mitochondria | -0.6 | -2.6 | -0.2 | -1.3 | -1.8 | -1.7 | -0.6 | 0.5 | 0.9 | -3.2 | -8.3 | -6.5 | -12.1 | -15.0 | -13.4 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 1.3 | 4.2 | 4.9 | 5.3 | 4.3 | 4.3 | 3.4 | 4.4 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 2.3 | 4.9 | 4.8 | 4.0 | 2.9 | 2.9 | 2.3 | 2.3 | -1.1 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | -1.2 | 0 | -2.2 | 0 | -2.3 | 0 | 0 | 0 | -1.1 | -2.3 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | -3.2 | -0.3 | -1.9 | -2.8 | -1.5 | -1.2 | -0.7 | -1.1 | 0.4 | 3.3 | 6.5 | 4.4 | 0 | 6.2 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 8.7795 | 8.9542 | 9.3934 | 9.321 | 8.6666 | 9.196 | 7.559 | 8.3157 | 8.0436 | 7.2683 | 7.411 | 7.8881 | 6.372 | 7.2899 | 6.8466 | 6.6322 | 5.1987 | 7.0715 |
Actin | 0.0062 | 0.0231 | 0.0195 | 0.0001 | 0.0001 | 0.0004 | 0.0468 | 0.0071 | 0.0088 | 0.0122 | 0.0567 | 0.002 | 0.0429 | 0.0175 | 0.0011 | 0.0148 | 0.0789 | 0.0014 |
Bud | 0.0005 | 0.002 | 0.0031 | 0.0001 | 0.0001 | 0 | 0.0015 | 0.0008 | 0.0006 | 0.0027 | 0.0004 | 0.0001 | 0.0006 | 0.0003 | 0.0002 | 0.0014 | 0.0014 | 0.0001 |
Bud Neck | 0.0004 | 0.0008 | 0.001 | 0 | 0 | 0.0001 | 0.0011 | 0.0002 | 0.0002 | 0.0002 | 0.0001 | 0.0001 | 0.0014 | 0.0006 | 0.0004 | 0.0001 | 0.0015 | 0 |
Bud Periphery | 0.0009 | 0.0028 | 0.0034 | 0.0005 | 0.0009 | 0.0002 | 0.0041 | 0.0012 | 0.0012 | 0.0061 | 0.0018 | 0.0004 | 0.001 | 0.0005 | 0.0006 | 0.0026 | 0.0031 | 0.0001 |
Bud Site | 0.0014 | 0.0213 | 0.0038 | 0.0001 | 0 | 0 | 0.0061 | 0.0023 | 0.0031 | 0.0009 | 0.0002 | 0.0002 | 0.0037 | 0.003 | 0.0003 | 0.0008 | 0.0059 | 0 |
Cell Periphery | 0.0003 | 0.0013 | 0.0006 | 0.0003 | 0.0002 | 0.0001 | 0.0008 | 0.0006 | 0.0003 | 0.0013 | 0.0003 | 0.0001 | 0.0004 | 0.0015 | 0.0006 | 0.0001 | 0.0006 | 0.0001 |
Cytoplasm | 0.0006 | 0.0003 | 0.0006 | 0 | 0 | 0.0001 | 0.0024 | 0.0019 | 0.0003 | 0.0003 | 0.0001 | 0.0001 | 0.01 | 0 | 0 | 0.0001 | 0.0027 | 0.0002 |
Cytoplasmic Foci | 0.0066 | 0.0075 | 0.0065 | 0.0001 | 0.0001 | 0.0005 | 0.0069 | 0.0032 | 0.003 | 0.0012 | 0.0012 | 0.0011 | 0.0117 | 0.0023 | 0.0004 | 0.006 | 0.0446 | 0.0006 |
Eisosomes | 0.0002 | 0.0004 | 0.0015 | 0 | 0 | 0.0001 | 0.0009 | 0.0004 | 0.0003 | 0.0002 | 0.0001 | 0.0002 | 0.0011 | 0.0005 | 0.0001 | 0.0004 | 0.0009 | 0.0001 |
Endoplasmic Reticulum | 0.0004 | 0.0002 | 0.0006 | 0 | 0 | 0.0001 | 0.0044 | 0.0007 | 0.0003 | 0.0004 | 0.0001 | 0.0007 | 0.0031 | 0.0001 | 0.0001 | 0 | 0.0041 | 0.0003 |
Endosome | 0.0304 | 0.0205 | 0.0078 | 0.002 | 0.0005 | 0.006 | 0.0276 | 0.0134 | 0.0056 | 0.0105 | 0.0036 | 0.0076 | 0.0191 | 0.0019 | 0.005 | 0.0067 | 0.0351 | 0.004 |
Golgi | 0.0313 | 0.0419 | 0.0102 | 0.0008 | 0.0007 | 0.0097 | 0.0185 | 0.0245 | 0.0134 | 0.0179 | 0.0034 | 0.0175 | 0.0129 | 0.0028 | 0.0053 | 0.0332 | 0.0198 | 0.0046 |
Lipid Particles | 0.0074 | 0.0212 | 0.0074 | 0.0013 | 0.0003 | 0.0024 | 0.0152 | 0.0021 | 0.0026 | 0.001 | 0.0031 | 0.0026 | 0.0204 | 0.0138 | 0.0038 | 0.0023 | 0.0252 | 0.0008 |
Mitochondria | 0.8907 | 0.8197 | 0.9081 | 0.979 | 0.9879 | 0.9616 | 0.8182 | 0.9111 | 0.9413 | 0.9374 | 0.8752 | 0.9587 | 0.8273 | 0.9339 | 0.9697 | 0.9241 | 0.6471 | 0.9836 |
None | 0.0001 | 0.0001 | 0.0106 | 0 | 0 | 0.0021 | 0.0016 | 0.0004 | 0.0002 | 0.0001 | 0 | 0.0001 | 0.0088 | 0.0001 | 0 | 0.0001 | 0.0036 | 0.0001 |
Nuclear Periphery | 0.0001 | 0.0002 | 0.0004 | 0 | 0 | 0.0003 | 0.0068 | 0.0002 | 0.0019 | 0.0003 | 0.0002 | 0.0024 | 0.0058 | 0.0005 | 0 | 0 | 0.0208 | 0.0013 |
Nucleolus | 0.0003 | 0.0004 | 0.0004 | 0.0003 | 0 | 0.0033 | 0.0016 | 0.004 | 0.0009 | 0.0002 | 0.0002 | 0.0001 | 0.0009 | 0.0004 | 0.0007 | 0.0001 | 0.0063 | 0.0001 |
Nucleus | 0.0002 | 0.0002 | 0.0003 | 0.0001 | 0 | 0.0019 | 0.0025 | 0.0015 | 0.0017 | 0.0002 | 0.0002 | 0.0002 | 0.0043 | 0.0002 | 0.0002 | 0.0001 | 0.0071 | 0.0007 |
Peroxisomes | 0.0083 | 0.011 | 0.0079 | 0.0007 | 0.0017 | 0.0012 | 0.0104 | 0.0029 | 0.0025 | 0.0008 | 0.0064 | 0.0011 | 0.0132 | 0.0044 | 0.0012 | 0.0044 | 0.0049 | 0.0005 |
Punctate Nuclear | 0.0001 | 0 | 0.0005 | 0 | 0 | 0.0021 | 0.0027 | 0.0002 | 0.0024 | 0 | 0 | 0.001 | 0.0069 | 0.0026 | 0 | 0 | 0.0408 | 0.0001 |
Vacuole | 0.0035 | 0.0079 | 0.0022 | 0.0079 | 0.002 | 0.0017 | 0.0083 | 0.0096 | 0.0025 | 0.0029 | 0.013 | 0.0006 | 0.0017 | 0.0038 | 0.0053 | 0.0007 | 0.0148 | 0.0004 |
Vacuole Periphery | 0.0101 | 0.017 | 0.0035 | 0.0065 | 0.0051 | 0.0062 | 0.0118 | 0.0117 | 0.0073 | 0.0032 | 0.0337 | 0.0032 | 0.0028 | 0.0093 | 0.0049 | 0.0021 | 0.0309 | 0.001 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 28.324 | 32.2921 | 29.9526 | 20.9105 | 14.37 | 24.6159 | 23.1893 | 27.0453 | 22.3526 | 22.6531 |
Translational Efficiency | 0.8874 | 0.7958 | 0.7617 | 1.1198 | 1.4723 | 0.695 | 0.9765 | 0.7106 | 0.7918 | 0.7499 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
250 | 234 | 1573 | 989 | 1502 | 1478 | 330 | 66 | 1752 | 1712 | 1903 | 1055 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 1138.25 | 1233.76 | 1342.50 | 1191.46 | 1078.40 | 1174.16 | 1354.69 | 1198.61 | 1086.94 | 1182.31 | 1344.61 | 1191.91 |
Standard Deviation | 214.43 | 214.74 | 221.99 | 203.91 | 197.13 | 188.82 | 237.04 | 232.87 | 200.78 | 193.65 | 224.72 | 205.85 |
Intensity Change Log 2 | — | 0.116244 | 0.238105 | 0.065913 | — | 0.122737 | 0.329070 | 0.152470 | — | 0.119406 | 0.283076 | 0.108672 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.001226 | 0.001248 | 0.001729 | 0.004335 | 0.000470 | 0.003010 | 0.003014 | 0.005843 | 0.000578 | 0.002769 | 0.001952 | 0.004429 |
Bud Neck | 0.000319 | 0.003311 | 0.001775 | 0.003146 | 0.000600 | 0.000742 | 0.010411 | 0.010264 | 0.000560 | 0.001093 | 0.003273 | 0.003591 |
Bud Site | 0.002416 | 0.008675 | 0.004122 | 0.035051 | 0.000999 | 0.002757 | 0.018229 | 0.107576* | 0.001202 | 0.003566 | 0.006569 | 0.039588 |
Cell Periphery | 0.002938 | 0.000272 | 0.000249 | 0.001521 | 0.000214 | 0.000271 | 0.000859 | 0.001004 | 0.000603 | 0.000271 | 0.000355 | 0.001489 |
Cytoplasm | 0.002274 | 0.000835 | 0.000629 | 0.004686 | 0.000111 | 0.000291 | 0.001336 | 0.012522 | 0.000420 | 0.000365 | 0.000751 | 0.005176 |
Cytoplasmic Foci | 0.012365 | 0.018981 | 0.013949 | 0.021887 | 0.007527 | 0.010357 | 0.024010 | 0.056274 | 0.008217 | 0.011535 | 0.015694 | 0.024038 |
Eisosomes | 0.000096 | 0.000391 | 0.000059 | 0.000103 | 0.000057 | 0.000062 | 0.000096 | 0.000153 | 0.000062 | 0.000107 | 0.000066 | 0.000106 |
Endoplasmic Reticulum | 0.005057 | 0.000424 | 0.000280 | 0.001394 | 0.000159 | 0.000269 | 0.000841 | 0.002477 | 0.000858 | 0.000290 | 0.000377 | 0.001461 |
Endosome | 0.008398 | 0.014741 | 0.009780 | 0.029866 | 0.004187 | 0.010529 | 0.045826 | 0.072622 | 0.004788 | 0.011105 | 0.016031 | 0.032541 |
Golgi | 0.065949 | 0.027205 | 0.031524 | 0.116565* | 0.010735 | 0.043966 | 0.038554 | 0.158653* | 0.018614 | 0.041675 | 0.032743 | 0.119198* |
Lipid Particles | 0.002256 | 0.001187 | 0.002146 | 0.000536 | 0.001580 | 0.001471 | 0.007161 | 0.000838 | 0.001676 | 0.001432 | 0.003015 | 0.000554 |
Mitochondria | 0.859340* | 0.864599* | 0.856227* | 0.635636* | 0.951430* | 0.889946* | 0.699396* | 0.360921* | 0.938289* | 0.886482* | 0.829031* | 0.618450* |
Mitotic Spindle | 0.001077 | 0.002979 | 0.009471 | 0.031643 | 0.000619 | 0.001577 | 0.037820 | 0.028516 | 0.000684 | 0.001769 | 0.014387 | 0.031448 |
None | 0.001309 | 0.001298 | 0.001069 | 0.000892 | 0.001050 | 0.000560 | 0.000787 | 0.000558 | 0.001087 | 0.000661 | 0.001020 | 0.000871 |
Nuclear Periphery | 0.000209 | 0.001243 | 0.001693 | 0.000344 | 0.000208 | 0.000140 | 0.002410 | 0.000302 | 0.000209 | 0.000291 | 0.001817 | 0.000341 |
Nuclear Periphery Foci | 0.000286 | 0.001334 | 0.000681 | 0.000494 | 0.000187 | 0.000134 | 0.002030 | 0.000349 | 0.000201 | 0.000298 | 0.000915 | 0.000485 |
Nucleolus | 0.000079 | 0.001568 | 0.000287 | 0.000293 | 0.000246 | 0.000147 | 0.002093 | 0.000251 | 0.000222 | 0.000342 | 0.000600 | 0.000291 |
Nucleus | 0.000077 | 0.000338 | 0.000125 | 0.000400 | 0.000091 | 0.000061 | 0.000844 | 0.001070 | 0.000089 | 0.000099 | 0.000250 | 0.000442 |
Peroxisomes | 0.000732 | 0.008092 | 0.004555 | 0.005316 | 0.004087 | 0.004645 | 0.019312 | 0.016977 | 0.003609 | 0.005116 | 0.007114 | 0.006046 |
Vacuole | 0.005827 | 0.019508 | 0.019751 | 0.045331 | 0.004375 | 0.012420 | 0.041019 | 0.117044 | 0.004582 | 0.013389 | 0.023439 | 0.049818 |
Vacuole Periphery | 0.027769 | 0.021771 | 0.039899 | 0.060563 | 0.011068 | 0.016645 | 0.043952 | 0.045786 | 0.013451 | 0.017346 | 0.040601 | 0.059638 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -0.05 | -1.23 | -3.59 | -3.45 | -2.93 | -3.40 | -4.00 | -1.98 | -1.01 | -1.02 | -3.38 | -4.53 | -4.80 | -1.65 | -2.93 |
Bud Neck | -2.92 | -6.81 | -6.39 | 0.08 | -2.88 | -0.74 | -4.34 | -3.76 | -3.70 | 0.04 | -2.42 | -6.12 | -6.65 | -5.21 | -0.59 |
Bud Site | -1.83 | -1.38 | -9.65 | -5.82 | -9.30 | -2.78 | -4.31 | -4.14 | -4.07 | -3.41 | -3.28 | -5.42 | -11.07 | -10.25 | -9.21 |
Cell Periphery | 2.67 | 2.69 | 1.38 | -4.65 | -4.74 | -1.14 | -1.80 | -3.24 | -3.02 | -0.29 | 2.21 | 1.46 | -3.08 | -4.93 | -4.39 |
Cytoplasm | 0.84 | 0.96 | -1.55 | -3.94 | -4.26 | -4.52 | -3.28 | -1.54 | -1.53 | -1.45 | 0.22 | -1.03 | -4.43 | -4.54 | -4.17 |
Cytoplasmic Foci | -1.41 | -0.58 | -3.04 | -0.66 | -3.51 | -2.09 | -4.82 | -4.39 | -4.14 | -2.86 | -2.49 | -5.19 | -7.57 | -5.78 | -3.75 |
Eisosomes | -0.98 | 2.48 | -0.27 | 0.95 | -1.84 | -0.79 | -3.69 | -2.46 | -2.34 | -1.43 | -1.06 | -0.66 | -1.98 | 0.01 | -1.81 |
Endoplasmic Reticulum | 1.47 | 1.51 | 1.16 | -4.01 | -5.66 | -3.08 | -5.59 | -1.82 | -1.73 | -1.27 | 1.25 | 1.06 | -1.23 | -5.88 | -5.40 |
Endosome | -2.07 | -0.77 | -8.51 | -4.72 | -9.75 | -5.72 | -7.65 | -4.33 | -3.92 | -1.54 | -6.04 | -8.49 | -12.98 | -9.62 | -6.94 |
Golgi | 3.52 | 3.38 | -4.26 | -11.27 | -12.64 | -10.09 | -5.73 | -5.26 | -4.03 | -4.19 | -7.05 | -5.23 | -15.51 | -11.40 | -13.23 |
Lipid Particles | 0.98 | 0.09 | 1.61 | 1.85 | 4.97 | 0.17 | -2.09 | 1.29 | 0.74 | 2.31 | 0.43 | -2.31 | 3.62 | 1.68 | 4.55 |
Mitochondria | -0.23 | 0.20 | 11.37 | 11.65 | 16.88* | 9.19 | 13.58 | 12.65 | 11.30 | 6.85 | 7.82 | 15.20 | 26.47 | 21.24 | 16.32* |
Mitotic Spindle | -1.62 | -5.68 | -8.84 | -8.01 | -6.09 | -2.34 | -6.15 | -2.03 | -1.97 | 0.43 | -2.79 | -8.59 | -9.23 | -8.88 | -4.80 |
None | 0.04 | 1.32 | 2.32 | 1.57 | 3.26 | 7.69 | 2.72 | 4.27 | 0.01 | 1.83 | 6.09 | 0.99 | 3.24 | -3.80 | 2.98 |
Nuclear Periphery | -1.50 | -4.97 | -1.62 | 1.31 | 4.59 | 1.55 | -5.64 | -0.66 | -1.15 | 5.07 | -0.81 | -6.40 | -2.43 | -0.53 | 5.83 |
Nuclear Periphery Foci | -1.38 | -2.13 | -1.37 | 1.05 | 1.02 | 1.24 | -4.25 | -1.47 | -1.98 | 3.74 | -0.88 | -6.23 | -3.30 | -1.69 | 2.70 |
Nucleolus | -1.35 | -3.19 | -1.34 | 1.14 | -0.08 | 0.67 | -2.36 | -0.11 | -0.97 | 2.35 | -0.61 | -2.19 | -0.43 | 0.19 | 1.51 |
Nucleus | -1.15 | -1.03 | -1.19 | -0.66 | -1.10 | 0.70 | -4.74 | -2.14 | -2.19 | -0.98 | -0.21 | -3.55 | -1.34 | -1.31 | -1.02 |
Peroxisomes | -4.13 | -4.76 | -5.63 | 1.41 | -0.68 | -0.41 | -3.97 | -1.91 | -1.84 | 0.05 | -1.26 | -2.93 | -2.19 | -0.84 | 0.73 |
Vacuole | -3.33 | -6.62 | -11.20 | -5.26 | -7.30 | -5.10 | -8.20 | -4.75 | -4.41 | -3.17 | -5.97 | -12.10 | -13.18 | -10.02 | -7.22 |
Vacuole Periphery | 0.88 | -2.02 | -4.91 | -7.03 | -4.61 | -3.06 | -5.96 | -4.48 | -3.74 | -0.09 | -2.11 | -10.53 | -12.15 | -11.21 | -4.52 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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