Standard name
Human Ortholog
Description tRNA threonylcarbamoyladenosine dehydratase; required for the ct6A tRNA base modification, where an adenosine at position 37 is modified to form a cyclized active ester with an oxazolone ring; localized to the mitochondrial outer membrane; TCD2 has a paralog, TCD1, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0 0.05 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0.09 0.06 0.05 0 0 0 0 0 0
Cytoplasm 0 0 0 0.07 0.07 0 0 0 0 0 0 0 0 0 0 0.09 0.06 0.19 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.05 0.05 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0.23 0.43 0.6 0.3 0.22 0.14 0.05 0.1 0.05 0.12 0.12 0.1
Mitochondria 0.95 0.94 0.92 0.9 0.85 0.91 0.89 0.88 0.88 0.9 0.93 0.93 0.82 0.63 0.7 0.14 0.1 0 0.86 0.76 0.85 0.72 0.67 0.72
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0.06 0 0 0 0 0.1 0.12 0.13 0.1 0.1 0.07 0.1 0 0 0 0.07 0.06 0 0 0 0 0 0 0
Nucleolus 0.09 0.06 0 0 0.08 0.16 0.15 0.12 0.09 0.09 0.08 0.07 0 0 0 0.07 0.06 0.05 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.06 0.1 0 0 0.07 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.1 0.13 0.07 0 0.06 0 0 0 0.05 0.06 0.05 0.08 0.17 0.27 0.19 0.28 0.32 0.33 0 0.06 0 0.09 0.1 0.08
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 2 1 1 0 0 0 1 1 0 1 1 0 2 0 2 3 1 2 4 1 3 2 9 8
Bud 1 1 0 7 2 4 9 5 11 9 9 10 1 1 2 1 3 0 0 0 0 1 2 4
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 1 1
Bud Site 1 1 1 0 2 3 3 3 1 7 2 2 0 0 0 0 0 1 – – – – – –
Cell Periphery 4 4 10 2 1 3 3 1 1 2 2 3 7 20 12 4 6 2 0 0 0 0 0 0
Cytoplasm 0 0 5 12 21 11 13 10 16 12 7 2 5 10 5 4 6 8 0 0 0 0 1 0
Endoplasmic Reticulum 2 1 0 0 2 0 5 4 2 3 3 3 3 0 4 2 5 2 1 0 0 1 1 2
Endosome 1 0 1 0 4 1 2 9 4 1 3 7 0 6 3 2 2 0 4 2 1 4 6 4
Golgi 4 3 10 2 7 1 0 3 0 0 0 0 51 155 208 13 21 6 17 13 13 26 44 32
Mitochondria 315 118 247 155 238 264 369 378 373 560 351 542 183 224 243 6 10 1 286 98 239 157 251 227
Nucleus 0 1 0 0 1 1 3 4 6 2 1 4 1 1 0 0 3 1 0 0 0 1 3 0
Nuclear Periphery 19 3 4 4 9 28 48 56 41 60 28 57 7 2 2 3 6 1 2 0 0 0 2 1
Nucleolus 31 8 9 3 22 45 62 52 39 55 29 43 4 2 0 3 6 2 0 1 0 0 1 2
Peroxisomes 0 0 9 1 5 1 4 0 4 2 3 4 4 3 2 2 6 4 4 0 19 2 6 4
SpindlePole 0 1 0 0 1 0 2 4 1 6 1 5 1 0 0 1 1 1 0 0 0 0 1 0
Vac/Vac Membrane 34 16 19 1 18 10 11 19 21 36 19 45 37 98 67 12 31 14 6 7 2 18 36 24
Unique Cell Count 330 126 269 172 281 289 416 432 425 619 378 580 223 357 345 43 96 42 333 129 283 220 376 317
Labelled Cell Count 414 158 316 187 333 372 535 549 520 756 459 727 306 522 550 56 108 45 333 129 283 220 376 317


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 11.0 13.9 12.3 10.8 10.0 8.5 8.2 8.3 8.1 7.8 7.3 7.5 12.9 13.0 12.1 12.0 11.7 11.2 13.7 13.4 14.9
Std Deviation (1e-4) 1.9 3.3 2.8 2.7 2.4 2.6 2.1 2.5 2.4 2.3 2.0 1.9 2.5 2.2 2.1 3.3 3.0 3.8 2.8 3.2 3.0
Intensity Change (Log2) – – – -0.19 -0.3 -0.53 -0.59 -0.56 -0.61 -0.66 -0.75 -0.72 0.07 0.08 -0.02 -0.04 -0.07 -0.13 0.16 0.12 0.27

WT3RAP60RAP140RAP220RAP300RAP380RAP460RAP540RAP620RAP700051015WT3HU80HU120HU160051015WT3rpd3Δ_1rpd3Δ_2rpd3Δ_3051015WT1AF100AF140AF180051015
rapamycinhydroxy-urearpd3 knockoutalpha-factorMultiple Condition Time-Course Intensity PlotsGFP Intensity (1e-4)GFP Intensity (1e-4)GFP Intensity (1e-4)GFP Intensity (1e-4)

Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 -2.8 -2.1 -2.8 0 0 0 0 0 -0.3 1.1 -0.2 0 0 0
Cytoplasm 2.7 3.1 1.4 1.0 0.4 1.4 0.1 0 0 0 0.8 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 -0.8 -2.9 0 0 0 0 0 0 6.4 11.2 14.5 0 5.5 0
Mitochondria -0.6 -2.6 -0.2 -1.3 -1.8 -1.7 -0.6 0.5 0.9 -3.2 -8.3 -6.5 -12.1 -15.0 -13.4
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 1.3 4.2 4.9 5.3 4.3 4.3 3.4 4.4 0 0 0 0 0 0
Nucleolus 0 2.3 4.9 4.8 4.0 2.9 2.9 2.3 2.3 -1.1 0 0 0 0 0
Peroxisomes 0 -1.2 0 -2.2 0 -2.3 0 0 0 -1.1 -2.3 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole -3.2 -0.3 -1.9 -2.8 -1.5 -1.2 -0.7 -1.1 0.4 3.3 6.5 4.4 0 6.2 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 8.7795 8.9542 9.3934 9.321 8.6666 9.196 7.559 8.3157 8.0436 7.2683 7.411 7.8881 6.372 7.2899 6.8466 6.6322 5.1987 7.0715
Actin 0.0062 0.0231 0.0195 0.0001 0.0001 0.0004 0.0468 0.0071 0.0088 0.0122 0.0567 0.002 0.0429 0.0175 0.0011 0.0148 0.0789 0.0014
Bud 0.0005 0.002 0.0031 0.0001 0.0001 0 0.0015 0.0008 0.0006 0.0027 0.0004 0.0001 0.0006 0.0003 0.0002 0.0014 0.0014 0.0001
Bud Neck 0.0004 0.0008 0.001 0 0 0.0001 0.0011 0.0002 0.0002 0.0002 0.0001 0.0001 0.0014 0.0006 0.0004 0.0001 0.0015 0
Bud Periphery 0.0009 0.0028 0.0034 0.0005 0.0009 0.0002 0.0041 0.0012 0.0012 0.0061 0.0018 0.0004 0.001 0.0005 0.0006 0.0026 0.0031 0.0001
Bud Site 0.0014 0.0213 0.0038 0.0001 0 0 0.0061 0.0023 0.0031 0.0009 0.0002 0.0002 0.0037 0.003 0.0003 0.0008 0.0059 0
Cell Periphery 0.0003 0.0013 0.0006 0.0003 0.0002 0.0001 0.0008 0.0006 0.0003 0.0013 0.0003 0.0001 0.0004 0.0015 0.0006 0.0001 0.0006 0.0001
Cytoplasm 0.0006 0.0003 0.0006 0 0 0.0001 0.0024 0.0019 0.0003 0.0003 0.0001 0.0001 0.01 0 0 0.0001 0.0027 0.0002
Cytoplasmic Foci 0.0066 0.0075 0.0065 0.0001 0.0001 0.0005 0.0069 0.0032 0.003 0.0012 0.0012 0.0011 0.0117 0.0023 0.0004 0.006 0.0446 0.0006
Eisosomes 0.0002 0.0004 0.0015 0 0 0.0001 0.0009 0.0004 0.0003 0.0002 0.0001 0.0002 0.0011 0.0005 0.0001 0.0004 0.0009 0.0001
Endoplasmic Reticulum 0.0004 0.0002 0.0006 0 0 0.0001 0.0044 0.0007 0.0003 0.0004 0.0001 0.0007 0.0031 0.0001 0.0001 0 0.0041 0.0003
Endosome 0.0304 0.0205 0.0078 0.002 0.0005 0.006 0.0276 0.0134 0.0056 0.0105 0.0036 0.0076 0.0191 0.0019 0.005 0.0067 0.0351 0.004
Golgi 0.0313 0.0419 0.0102 0.0008 0.0007 0.0097 0.0185 0.0245 0.0134 0.0179 0.0034 0.0175 0.0129 0.0028 0.0053 0.0332 0.0198 0.0046
Lipid Particles 0.0074 0.0212 0.0074 0.0013 0.0003 0.0024 0.0152 0.0021 0.0026 0.001 0.0031 0.0026 0.0204 0.0138 0.0038 0.0023 0.0252 0.0008
Mitochondria 0.8907 0.8197 0.9081 0.979 0.9879 0.9616 0.8182 0.9111 0.9413 0.9374 0.8752 0.9587 0.8273 0.9339 0.9697 0.9241 0.6471 0.9836
None 0.0001 0.0001 0.0106 0 0 0.0021 0.0016 0.0004 0.0002 0.0001 0 0.0001 0.0088 0.0001 0 0.0001 0.0036 0.0001
Nuclear Periphery 0.0001 0.0002 0.0004 0 0 0.0003 0.0068 0.0002 0.0019 0.0003 0.0002 0.0024 0.0058 0.0005 0 0 0.0208 0.0013
Nucleolus 0.0003 0.0004 0.0004 0.0003 0 0.0033 0.0016 0.004 0.0009 0.0002 0.0002 0.0001 0.0009 0.0004 0.0007 0.0001 0.0063 0.0001
Nucleus 0.0002 0.0002 0.0003 0.0001 0 0.0019 0.0025 0.0015 0.0017 0.0002 0.0002 0.0002 0.0043 0.0002 0.0002 0.0001 0.0071 0.0007
Peroxisomes 0.0083 0.011 0.0079 0.0007 0.0017 0.0012 0.0104 0.0029 0.0025 0.0008 0.0064 0.0011 0.0132 0.0044 0.0012 0.0044 0.0049 0.0005
Punctate Nuclear 0.0001 0 0.0005 0 0 0.0021 0.0027 0.0002 0.0024 0 0 0.001 0.0069 0.0026 0 0 0.0408 0.0001
Vacuole 0.0035 0.0079 0.0022 0.0079 0.002 0.0017 0.0083 0.0096 0.0025 0.0029 0.013 0.0006 0.0017 0.0038 0.0053 0.0007 0.0148 0.0004
Vacuole Periphery 0.0101 0.017 0.0035 0.0065 0.0051 0.0062 0.0118 0.0117 0.0073 0.0032 0.0337 0.0032 0.0028 0.0093 0.0049 0.0021 0.0309 0.001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 28.324 32.2921 29.9526 20.9105 14.37 24.6159 23.1893 27.0453 22.3526 22.6531
Translational Efficiency 0.8874 0.7958 0.7617 1.1198 1.4723 0.695 0.9765 0.7106 0.7918 0.7499

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
250 234 1573 989 1502 1478 330 66 1752 1712 1903 1055

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 1138.25 1233.76 1342.50 1191.46 1078.40 1174.16 1354.69 1198.61 1086.94 1182.31 1344.61 1191.91
Standard Deviation 214.43 214.74 221.99 203.91 197.13 188.82 237.04 232.87 200.78 193.65 224.72 205.85
Intensity Change Log 2 — 0.116244 0.238105 0.065913 — 0.122737 0.329070 0.152470 — 0.119406 0.283076 0.108672

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.001226 0.001248 0.001729 0.004335 0.000470 0.003010 0.003014 0.005843 0.000578 0.002769 0.001952 0.004429
Bud Neck 0.000319 0.003311 0.001775 0.003146 0.000600 0.000742 0.010411 0.010264 0.000560 0.001093 0.003273 0.003591
Bud Site 0.002416 0.008675 0.004122 0.035051 0.000999 0.002757 0.018229 0.107576 0.001202 0.003566 0.006569 0.039588
Cell Periphery 0.002938 0.000272 0.000249 0.001521 0.000214 0.000271 0.000859 0.001004 0.000603 0.000271 0.000355 0.001489
Cytoplasm 0.002274 0.000835 0.000629 0.004686 0.000111 0.000291 0.001336 0.012522 0.000420 0.000365 0.000751 0.005176
Cytoplasmic Foci 0.012365 0.018981 0.013949 0.021887 0.007527 0.010357 0.024010 0.056274 0.008217 0.011535 0.015694 0.024038
Eisosomes 0.000096 0.000391 0.000059 0.000103 0.000057 0.000062 0.000096 0.000153 0.000062 0.000107 0.000066 0.000106
Endoplasmic Reticulum 0.005057 0.000424 0.000280 0.001394 0.000159 0.000269 0.000841 0.002477 0.000858 0.000290 0.000377 0.001461
Endosome 0.008398 0.014741 0.009780 0.029866 0.004187 0.010529 0.045826 0.072622 0.004788 0.011105 0.016031 0.032541
Golgi 0.065949 0.027205 0.031524 0.116565 0.010735 0.043966 0.038554 0.158653 0.018614 0.041675 0.032743 0.119198
Lipid Particles 0.002256 0.001187 0.002146 0.000536 0.001580 0.001471 0.007161 0.000838 0.001676 0.001432 0.003015 0.000554
Mitochondria 0.859340 0.864599 0.856227 0.635636 0.951430 0.889946 0.699396 0.360921 0.938289 0.886482 0.829031 0.618450
Mitotic Spindle 0.001077 0.002979 0.009471 0.031643 0.000619 0.001577 0.037820 0.028516 0.000684 0.001769 0.014387 0.031448
None 0.001309 0.001298 0.001069 0.000892 0.001050 0.000560 0.000787 0.000558 0.001087 0.000661 0.001020 0.000871
Nuclear Periphery 0.000209 0.001243 0.001693 0.000344 0.000208 0.000140 0.002410 0.000302 0.000209 0.000291 0.001817 0.000341
Nuclear Periphery Foci 0.000286 0.001334 0.000681 0.000494 0.000187 0.000134 0.002030 0.000349 0.000201 0.000298 0.000915 0.000485
Nucleolus 0.000079 0.001568 0.000287 0.000293 0.000246 0.000147 0.002093 0.000251 0.000222 0.000342 0.000600 0.000291
Nucleus 0.000077 0.000338 0.000125 0.000400 0.000091 0.000061 0.000844 0.001070 0.000089 0.000099 0.000250 0.000442
Peroxisomes 0.000732 0.008092 0.004555 0.005316 0.004087 0.004645 0.019312 0.016977 0.003609 0.005116 0.007114 0.006046
Vacuole 0.005827 0.019508 0.019751 0.045331 0.004375 0.012420 0.041019 0.117044 0.004582 0.013389 0.023439 0.049818
Vacuole Periphery 0.027769 0.021771 0.039899 0.060563 0.011068 0.016645 0.043952 0.045786 0.013451 0.017346 0.040601 0.059638

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -0.05 -1.23 -3.59 -3.45 -2.93 -3.40 -4.00 -1.98 -1.01 -1.02 -3.38 -4.53 -4.80 -1.65 -2.93
Bud Neck -2.92 -6.81 -6.39 0.08 -2.88 -0.74 -4.34 -3.76 -3.70 0.04 -2.42 -6.12 -6.65 -5.21 -0.59
Bud Site -1.83 -1.38 -9.65 -5.82 -9.30 -2.78 -4.31 -4.14 -4.07 -3.41 -3.28 -5.42 -11.07 -10.25 -9.21
Cell Periphery 2.67 2.69 1.38 -4.65 -4.74 -1.14 -1.80 -3.24 -3.02 -0.29 2.21 1.46 -3.08 -4.93 -4.39
Cytoplasm 0.84 0.96 -1.55 -3.94 -4.26 -4.52 -3.28 -1.54 -1.53 -1.45 0.22 -1.03 -4.43 -4.54 -4.17
Cytoplasmic Foci -1.41 -0.58 -3.04 -0.66 -3.51 -2.09 -4.82 -4.39 -4.14 -2.86 -2.49 -5.19 -7.57 -5.78 -3.75
Eisosomes -0.98 2.48 -0.27 0.95 -1.84 -0.79 -3.69 -2.46 -2.34 -1.43 -1.06 -0.66 -1.98 0.01 -1.81
Endoplasmic Reticulum 1.47 1.51 1.16 -4.01 -5.66 -3.08 -5.59 -1.82 -1.73 -1.27 1.25 1.06 -1.23 -5.88 -5.40
Endosome -2.07 -0.77 -8.51 -4.72 -9.75 -5.72 -7.65 -4.33 -3.92 -1.54 -6.04 -8.49 -12.98 -9.62 -6.94
Golgi 3.52 3.38 -4.26 -11.27 -12.64 -10.09 -5.73 -5.26 -4.03 -4.19 -7.05 -5.23 -15.51 -11.40 -13.23
Lipid Particles 0.98 0.09 1.61 1.85 4.97 0.17 -2.09 1.29 0.74 2.31 0.43 -2.31 3.62 1.68 4.55
Mitochondria -0.23 0.20 11.37 11.65 16.88 9.19 13.58 12.65 11.30 6.85 7.82 15.20 26.47 21.24 16.32
Mitotic Spindle -1.62 -5.68 -8.84 -8.01 -6.09 -2.34 -6.15 -2.03 -1.97 0.43 -2.79 -8.59 -9.23 -8.88 -4.80
None 0.04 1.32 2.32 1.57 3.26 7.69 2.72 4.27 0.01 1.83 6.09 0.99 3.24 -3.80 2.98
Nuclear Periphery -1.50 -4.97 -1.62 1.31 4.59 1.55 -5.64 -0.66 -1.15 5.07 -0.81 -6.40 -2.43 -0.53 5.83
Nuclear Periphery Foci -1.38 -2.13 -1.37 1.05 1.02 1.24 -4.25 -1.47 -1.98 3.74 -0.88 -6.23 -3.30 -1.69 2.70
Nucleolus -1.35 -3.19 -1.34 1.14 -0.08 0.67 -2.36 -0.11 -0.97 2.35 -0.61 -2.19 -0.43 0.19 1.51
Nucleus -1.15 -1.03 -1.19 -0.66 -1.10 0.70 -4.74 -2.14 -2.19 -0.98 -0.21 -3.55 -1.34 -1.31 -1.02
Peroxisomes -4.13 -4.76 -5.63 1.41 -0.68 -0.41 -3.97 -1.91 -1.84 0.05 -1.26 -2.93 -2.19 -0.84 0.73
Vacuole -3.33 -6.62 -11.20 -5.26 -7.30 -5.10 -8.20 -4.75 -4.41 -3.17 -5.97 -12.10 -13.18 -10.02 -7.22
Vacuole Periphery 0.88 -2.02 -4.91 -7.03 -4.61 -3.06 -5.96 -4.48 -3.74 -0.09 -2.11 -10.53 -12.15 -11.21 -4.52
Protein Complexes26S Proteasome complex37S mitochondrial small ribosomal subunit54S mitochondrial large ribosomal subunit60S cytosolic large ribosomal subunitAar2-U5 snRNPAnaphase-Promoting Complex variant 1Anaphase-Promoting Complex variant 2Anaphase-Promoting Complex variant 3Anaphase-Promoting Complex variant 4Arp2/3 complexATG1 kinase complexBLOC-1 complexCcr4-Not complexCentral kinetochore CTF19 complexCleavage and polyadenylation specificity factorcomplexCMG helicase complexCMG-Pol epsilon complexCOMPASS complexCOP9 signalosome COPI vesicle coat complexCoQ6 biosynthesis complexCore mediator complexCORVET complexCTF18-RFC complexCURI complex variant 1CURI complex variant 2CURI complex variant 3Cytoplasmic exosome complex, DIS3 variantDASH complexDNA-directed RNA Polymerase I complexDNA-directed RNA polymerase II complexDNA-directed RNA polymerase III complexDNA replication factor C complexEGO complexELG1-RFC complex Elongator holoenzyme complexEndoplasmic Reticulum Membrane ComplexEukaryotic translation initiation factor 2BcomplexEukaryotic translation initiation factor 3core complexExocystExomer complexFAR complexGeneral transcription factor complex TFIIDGeneral transcription factor complex TFIIHGET4-GET5 transmembrane domain recognition complexGID ubiquitin ligase complexGin4 complexGolgi transport complexGSE complexHMC complexHOPS complexINO80 chromatin remodeling complexLSM1-7 complexLsm1-7-Pat1 complexLSM2-7 complexLSM2-8 complexMCM complexMICOS complexMitochondrial electron transport complex IIIMitochondrial inner membrane pre-sequence translocasecomplexMitochondrial outer membrane translocase holocomplexMitochondrial proton-transporting ATP synthase complexMitochondrial pyruvate dehydrogenase complexNineTeen complexNOP8 60s ribosome pre-assembly complexNuA3 histone acetyltransferase complexNuA4 histone acetyltransferase complexNuclear condensin complexNuclear/nucleolar exosome complex, DIS3-RRP6 variantNuclear pore complexNucleolar ribonuclease MRP complexNucleolar ribonuclease P complexOligosaccharyl transferase complex variant 1Oligosaccharyl transferase complex variant 2PAF1 complexPAS complexPhosphatidylinositol 3-kinase complex IPolarisomePrefoldin complexPRP19-associated complexRad17 RFC-like complexRetromer complexRPD3L histone deacetylase complexRPD3S histone deacetylase complexRQC complexRSC complex variant RSC1RSC complex variant RSC2SAGA complexSCF-Met30 ubiquitin ligase complexSCF-Ufo1 ubiquitin ligase complexSEA complexSeptin complexSET3C histone deacetylase complexSignal recognition particleSLIK (SAGA-like) complexSmall ribosomal subunit processome, variant1Small ribosomal subunit processome, variant2Small ribosomal subunit processome, variant3SMC5-SMC6 SUMO ligase complexSm complexSpliceosomal commitment complexSPOTS complexSWI/SNF chromatin remodelling complexSwr1 chromatin remodelling complexTHO complexTranscription factor TFIIIC complexTranslocon complexTRAPPIII protein complexTRAPPII protein complexTRAPPI protein complexTREX complexU1 snRNPU2 snRNPU4 snRNPU4/U6 snRNPU4/U6.U5 tri-snRNP complexU5 snRNPU6 snRNP complexUTP-A complexUTP-B complexUTP-C complex variant 1UTP-C complex variant 2UTP-C complex variant 3Vacuolar proton translocating ATPase complex,Golgi variantVacuolar proton translocating ATPase complex,vacuole variantCellular Components••••••••••••••••actinbud neckcell peripherycytoplasmendosomeERGolgimitochondrionmixednuclear peripherynucleolusnucleusperoxisomespindle polevacuolar membranevacuole
Standard name
Human Ortholog
Description tRNA threonylcarbamoyladenosine dehydratase; required for the ct6A tRNA base modification, where an adenosine at position 37 is modified to form a cyclized active ester with an oxazolone ring; localized to the mitochondrial outer membrane; TCD2 has a paralog, TCD1, that arose from the whole genome duplication
Localization
Cell Percentages mitochondrion (98%)
Cell Cycle Regulation No
Subcompartmental Group mito-2

Tcd2

Tcd2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Tcd2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available