Standard name
Human Ortholog
Description Transcriptional repressor that regulates hypoxic genes during normoxia; involved in the aerobic repression of genes such as COX5b, TIR1, and HEM13; binds DNA intrastrand cross-links formed by cisplatin; HMG (high mobility group box) domain containing protein which binds and bends cisplatin-modified DNA, blocking excision repair; IXR1 has a paralog, ABF2, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches – 0 0 0 0 0 0 – 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0
Bud – 0 0 0 0 0 0 – 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0
Bud Neck – 0 0 0 0 0 0 – 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0
Bud Site – 0 0 0 0 0 0 – 0 0 0 0.06 – – – 0 0 0 – – – – – –
Cell Periphery – 0 0 0 0 0 0 – 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0
Cytoplasm – 0 0 0 0.11 0 0 – 0 0.07 0.05 0 – – – 0 0 0.07 0 0 0 0 0 0.06
Endoplasmic Reticulum – 0 0 0 0 0 0 – 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0
Endosome – 0 0 0 0 0 0 – 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0
Golgi – 0 0 0 0 0 0 – 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0
Mitochondria – 0 0 0 0 0 0 – 0 0 0.05 0 – – – 0 0 0 0 0 0 0 0 0
Nucleus – 0.93 0.79 0.91 0.81 0.73 0.76 – 0.65 0.67 0.73 0.71 – – – 0.89 0.78 0.74 0.85 0.85 0.78 0.58 0.73 0.67
Nuclear Periphery – 0 0 0 0 0 0 – 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0
Nucleolus – 0.22 0.43 0.2 0.12 0.41 0.27 – 0.56 0.41 0.52 0.59 – – – 0.16 0.17 0.16 0.13 0.09 0.15 0.35 0 0.06
Peroxisomes – 0 0 0 0 0 0 – 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0
SpindlePole – 0 0 0 0 0 0 – 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane – 0 0 0 0 0 0 – 0 0 0 0 – – – 0 0 0.05 0 0 0 0.07 0 0.05
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches – 0 0 0 0 0 1 – 0 0 0 0 – – – 0 1 0 0 0 0 0 0 0
Bud – 0 0 0 0 0 1 – 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0
Bud Neck – 0 0 0 0 0 1 – 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0
Bud Site – 0 0 0 1 1 0 – 3 0 0 8 – – – 0 1 0 – – – – – –
Cell Periphery – 0 0 0 0 0 0 – 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0
Cytoplasm – 2 1 3 15 5 9 – 5 14 4 2 – – – 4 9 10 0 0 1 0 0 1
Endoplasmic Reticulum – 0 0 0 0 0 0 – 0 0 0 0 – – – 0 2 3 0 0 0 0 0 0
Endosome – 0 0 0 0 1 0 – 0 0 0 0 – – – 0 2 4 0 2 0 0 0 0
Golgi – 0 0 0 0 1 1 – 0 1 0 0 – – – 1 0 1 0 2 0 0 0 0
Mitochondria – 3 0 1 0 3 3 – 4 5 4 2 – – – 0 2 1 0 0 0 0 0 0
Nucleus – 179 33 73 113 95 163 – 93 129 55 92 – – – 129 156 106 52 171 51 3 10 13
Nuclear Periphery – 0 0 0 0 1 1 – 0 1 0 0 – – – 0 0 0 0 0 0 0 0 0
Nucleolus – 42 18 16 17 53 58 – 80 79 39 76 – – – 23 35 23 7 17 9 2 0 1
Peroxisomes – 0 0 0 0 0 0 – 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0
SpindlePole – 0 0 0 0 0 0 – 0 0 0 0 – – – 0 2 0 0 1 0 0 0 0
Vac/Vac Membrane – 0 0 0 1 1 1 – 0 2 0 0 – – – 3 9 7 0 5 1 0 0 1
Unique Cell Count 193 42 80 139 130 214 144 193 75 129 145 200 144 61 203 66 6 15 20
Labelled Cell Count 226 52 93 147 161 239 185 231 102 180 160 219 155 61 203 66 6 15 20


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) – 19.7 12.3 14.9 17.9 13.9 14.7 – 11.2 14.1 13.5 11.7 – – – 21.1 21.6 19.4 – – –
Std Deviation (1e-4) – 5.0 3.0 5.5 8.7 6.0 5.2 – 3.4 7.3 6.2 3.7 – – – 6.8 7.4 5.6 – – –
Intensity Change (Log2) – – – 0.28 0.55 0.18 0.25 – -0.13 0.2 0.14 -0.07 – – – 0.78 0.82 0.66 – – –


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 – 0 0 0 0 – – – 0 0 0
Bud 0 0 0 0 – 0 0 0 0 – – – 0 0 0
Bud Neck 0 0 0 0 – 0 0 0 0 – – – 0 0 0
Bud Site 0 0 0 0 – 0 0 0 0 – – – 0 0 0
Cell Periphery 0 0 0 0 – 0 0 0 0 – – – 0 0 0
Cytoplasm 0 0 0 0 – 0 0 0 0 – – – 0 0 0
Endoplasmic Reticulum 0 0 0 0 – 0 0 0 0 – – – 0 0 0
Endosome 0 0 0 0 – 0 0 0 0 – – – 0 0 0
Golgi 0 0 0 0 – 0 0 0 0 – – – 0 0 0
Mitochondria 0 0 0 0 – 0 0 0 0 – – – 0 0 0
Nucleus 2.0 0.4 -0.7 -0.3 – -1.7 -1.5 -0.6 -0.9 – – – 1.7 -0.1 -0.7
Nuclear Periphery 0 0 0 0 – 0 0 0 0 – – – 0 0 0
Nucleolus -2.7 -4.4 -0.2 -2.0 – 1.5 -0.2 0.9 1.8 – – – -3.7 -3.6 -3.7
Peroxisomes 0 0 0 0 – 0 0 0 0 – – – 0 0 0
SpindlePole 0 0 0 0 – 0 0 0 0 – – – 0 0 0
Vacuole 0 0 0 0 – 0 0 0 0 – – – 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 14.0021 13.0088 12.5869 11.9174 11.2143 12.759 14.1226 12.4704 11.5389 11.9846 9.4056 10.7411 16.21 13.9731 12.4965 13.1453 12.8913 13.4577
Actin 0.0123 0 0.0068 0.0172 0.0007 0 0.0009 0 0.0107 0 0.0343 0.0001 0.0343 0 0.0196 0.0003 0 0.0013
Bud 0.0008 0 0.0002 0.0002 0.0002 0.0001 0.0002 0 0.0002 0 0.0008 0.0003 0.0003 0 0.0003 0.0002 0.0001 0.0002
Bud Neck 0.0026 0.0001 0.0008 0.0004 0.0004 0.0006 0.0001 0.0001 0.0001 0 0.0009 0.0007 0.0008 0 0.0006 0.0001 0.0002 0.0004
Bud Periphery 0.0016 0 0.0002 0.0005 0.0004 0.0001 0.0004 0 0.0003 0 0.0021 0.0014 0.0006 0 0.0007 0.0003 0.0002 0.0003
Bud Site 0.0062 0 0.0069 0.0005 0.0003 0.0001 0.0003 0.0001 0.0006 0 0.0011 0.0001 0.0025 0 0.0011 0.0002 0.0001 0.0001
Cell Periphery 0.0015 0 0.0003 0.0005 0.0003 0.0001 0.0004 0.0001 0.0002 0 0.0003 0.0006 0.0003 0 0.0001 0.0005 0.0002 0.0002
Cytoplasm 0.0019 0 0.0006 0.0001 0.0008 0.0001 0.0015 0 0.0001 0 0.0006 0.0002 0.0027 0 0.0002 0.0003 0 0.0011
Cytoplasmic Foci 0.0098 0 0.0058 0.004 0.0007 0.0037 0.0116 0 0.0061 0 0.0227 0.0033 0.0099 0 0.0034 0.0213 0 0.0002
Eisosomes 0.0005 0 0 0.0001 0 0 0 0 0.0002 0 0.0003 0 0.0008 0 0.0002 0 0 0
Endoplasmic Reticulum 0.0046 0 0.0004 0.0002 0.0006 0 0.0005 0 0.0001 0 0.0019 0.0003 0.002 0 0.0002 0.0001 0 0.0005
Endosome 0.0101 0 0.0062 0.0032 0.0073 0.0005 0.0087 0 0.0012 0 0.0493 0.0007 0.0071 0 0.0064 0.0008 0 0.0038
Golgi 0.0038 0 0.0038 0.012 0.0005 0.0002 0.001 0 0.0017 0 0.0199 0.0002 0.0046 0 0.0032 0.0011 0 0.0032
Lipid Particles 0.0127 0 0.0091 0.0024 0.0009 0.0046 0.0065 0 0.0131 0 0.007 0.0062 0.0051 0 0.0021 0.0236 0.0001 0.0037
Mitochondria 0.0162 0.0001 0.0051 0.0071 0.0024 0.0006 0.0026 0.0004 0.0156 0 0.0072 0.0033 0.0059 0.0003 0.0327 0.0034 0.0016 0.0027
None 0.0015 0 0.0005 0.0001 0.0006 0.0001 0.002 0 0.0002 0 0.0002 0.0002 0.0018 0 0.0001 0.0006 0 0.0001
Nuclear Periphery 0.0212 0.0011 0.0022 0.0017 0.0213 0.0003 0.0045 0.0017 0.003 0 0.0014 0.0011 0.0019 0.0004 0.0003 0.0008 0.0001 0.0035
Nucleolus 0.1369 0.0431 0.0385 0.0374 0.4267 0.2097 0.0464 0.0734 0.0615 0.0194 0.1542 0.1283 0.0534 0.0492 0.0247 0.061 0.3621 0.1198
Nucleus 0.7244 0.9552 0.905 0.8621 0.5274 0.7755 0.888 0.9235 0.8725 0.9805 0.6915 0.8416 0.8582 0.9498 0.891 0.8676 0.6341 0.8564
Peroxisomes 0.0055 0 0.0015 0.0293 0.0003 0.0019 0.0104 0 0.0104 0 0.001 0.0002 0.0038 0 0.004 0.0043 0.0001 0.0001
Punctate Nuclear 0.0097 0.0003 0.0049 0.0008 0.003 0.0011 0.0113 0.0005 0.0013 0 0.0006 0.0013 0.0026 0.0002 0.0001 0.0101 0.0006 0.0003
Vacuole 0.012 0.0001 0.0009 0.0057 0.0027 0.0004 0.0013 0.0001 0.0005 0 0.0017 0.0064 0.0008 0 0.005 0.0015 0.0002 0.0011
Vacuole Periphery 0.0042 0 0.0005 0.0144 0.0026 0.0002 0.0013 0.0002 0.0005 0 0.001 0.0035 0.0006 0.0001 0.0038 0.0019 0.0002 0.001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 18.7591 34.6386 19.5835 26.6892 27.113 20.2855 38.3586 30.0168 26.4406 35.8384
Translational Efficiency 2.5054 2.1498 2.436 1.345 2.3186 2.3603 1.5125 1.7848 1.9762 1.8655

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1087 1343 280 1480 2003 1950 263 2088 3090 3293 543 3568

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 792.01 1227.67 1998.15 1522.83 1235.54 1325.97 1704.56 1625.01 1079.52 1285.88 1855.95 1582.63
Standard Deviation 142.49 306.04 346.26 349.13 260.20 277.31 280.65 352.96 309.66 293.38 348.57 354.96
Intensity Change Log 2 — 0.632332 1.335074 0.943164 — 0.101906 0.464258 0.395307 — 0.332818 0.868844 0.634625

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000709 0.000652 0.001184 0.001118 0.000490 0.000531 0.000809 0.000761 0.000567 0.000580 0.001002 0.000909
Bud Neck 0.001763 0.002919 0.012802 0.015830 0.003771 0.005377 0.009930 0.020034 0.003064 0.004374 0.011411 0.018290
Bud Site 0.001569 0.000709 0.001387 0.002408 0.000413 0.000778 0.000862 0.001836 0.000820 0.000750 0.001133 0.002073
Cell Periphery 0.000236 0.000181 0.000024 0.000124 0.000081 0.000120 0.000034 0.000103 0.000136 0.000145 0.000029 0.000111
Cytoplasm 0.005845 0.000817 0.000020 0.001357 0.000395 0.000200 0.000155 0.001644 0.002312 0.000452 0.000086 0.001525
Cytoplasmic Foci 0.000528 0.000123 0.000369 0.001120 0.000026 0.000247 0.000167 0.000313 0.000202 0.000197 0.000271 0.000648
Eisosomes 0.000049 0.000131 0.000205 0.000045 0.000178 0.000218 0.000107 0.000041 0.000133 0.000183 0.000157 0.000042
Endoplasmic Reticulum 0.002772 0.000903 0.000442 0.000657 0.000923 0.001055 0.000527 0.000668 0.001573 0.000993 0.000483 0.000664
Endosome 0.000311 0.000130 0.000013 0.000655 0.000033 0.000348 0.000017 0.000419 0.000131 0.000259 0.000015 0.000517
Golgi 0.000262 0.000092 0.000104 0.000841 0.000031 0.000170 0.000079 0.000382 0.000112 0.000138 0.000092 0.000572
Lipid Particles 0.000078 0.000066 0.000019 0.000233 0.000005 0.000044 0.000012 0.000024 0.000031 0.000053 0.000016 0.000111
Mitochondria 0.000842 0.000868 0.002809 0.003100 0.000715 0.001024 0.001587 0.002530 0.000760 0.000960 0.002217 0.002767
Mitotic Spindle 0.000179 0.000705 0.000214 0.004280 0.000069 0.000745 0.003648 0.002548 0.000108 0.000729 0.001878 0.003267
None 0.015682 0.004864 0.000542 0.002214 0.000894 0.000679 0.001096 0.001354 0.006096 0.002386 0.000810 0.001710
Nuclear Periphery 0.000030 0.000050 0.000042 0.000505 0.000026 0.000100 0.000054 0.000445 0.000027 0.000080 0.000048 0.000470
Nuclear Periphery Foci 0.000482 0.000611 0.000007 0.000549 0.000030 0.000092 0.000012 0.000151 0.000189 0.000304 0.000009 0.000316
Nucleolus 0.065055 0.060784 0.136188 0.083657 0.075897 0.057031 0.073954 0.069902 0.072083 0.058561 0.106045 0.075608
Nucleus 0.900780* 0.922024* 0.824989* 0.877302* 0.913781* 0.928157* 0.901059* 0.894573* 0.909207* 0.925656* 0.861833* 0.887409*
Peroxisomes 0.001415 0.002090 0.017884 0.001635 0.001645 0.001461 0.005445 0.000985 0.001564 0.001718 0.011859 0.001255
Vacuole 0.001129 0.000802 0.000452 0.001652 0.000338 0.000889 0.000289 0.000703 0.000616 0.000853 0.000373 0.001097
Vacuole Periphery 0.000284 0.000478 0.000303 0.000720 0.000259 0.000736 0.000158 0.000583 0.000268 0.000631 0.000233 0.000640

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 0.79 -6.25 -3.63 -3.91 -0.24 -1.84 -8.12 -8.32 -7.11 0.20 -0.30 -10.62 -6.71 -6.29 0.37
Bud Neck -5.86 -12.19 -17.37 -16.15 -4.29 -4.79 -9.34 -17.86 -15.98 -9.42 -5.97 -14.74 -24.23 -22.10 -9.74
Bud Site 2.13 -0.05 -2.07 -3.75 -2.33 -2.51 -4.66 -5.08 -3.87 -3.00 0.44 -3.32 -5.16 -5.38 -3.63
Cell Periphery 0.75 5.18 2.46 0.87 -5.17 -1.19 1.67 -0.74 0.47 -2.69 -0.28 4.85 0.84 0.97 -5.03
Cytoplasm 3.35 3.89 2.71 -1.75 -3.74 0.70 0.84 -2.36 -2.94 -3.00 3.33 4.00 0.99 -3.43 -4.56
Cytoplasmic Foci 1.65 0.76 -0.92 -2.42 -1.73 -1.52 -2.28 -1.61 -0.16 -0.02 0.49 -0.66 -1.67 -2.12 -1.45
Eisosomes -5.69 -13.24 0.92 5.92 13.52 -1.66 5.42 12.82 8.20 11.12 -3.18 -3.30 12.57 9.92 16.30
Endoplasmic Reticulum 8.72 11.84 10.58 1.70 -4.79 -1.29 5.87 4.00 3.39 -2.55 5.75 13.32 11.03 3.74 -5.14
Endosome 1.68 2.79 -0.81 -2.05 -2.72 -2.25 0.94 -1.78 -0.08 -1.83 -1.05 2.92 -2.04 -1.19 -3.13
Golgi 1.70 1.39 -1.29 -1.88 -1.78 -1.28 -4.33 -2.30 -1.10 -1.74 -0.24 -0.05 -2.36 -2.17 -2.40
Lipid Particles 0.39 2.62 -1.30 -1.41 -1.89 -2.06 -3.27 -3.69 0.70 -1.61 -1.08 1.49 -1.72 -1.21 -2.02
Mitochondria -0.22 -9.71 -11.02 -11.88 -1.89 -2.25 -9.35 -11.71 -8.09 -5.45 -2.17 -13.31 -16.31 -13.34 -4.31
Mitotic Spindle -1.02 -1.30 -4.73 -4.02 -4.58 -1.86 -1.25 -5.19 -4.35 -0.39 -2.06 -1.32 -7.02 -5.92 -1.98
None 4.24 6.61 5.79 2.14 -4.74 1.50 -0.76 -3.87 -6.02 -1.42 3.94 6.33 5.06 1.01 -4.67
Nuclear Periphery -2.02 -3.80 -6.78 -6.49 -6.54 -2.66 -3.33 -9.02 -6.86 -7.88 -3.09 -4.51 -11.11 -9.43 -10.27
Nuclear Periphery Foci -0.25 3.76 -0.42 -0.04 -1.92 -1.94 1.34 -3.96 -1.97 -4.49 -0.57 3.93 -1.24 -0.29 -2.61
Nucleolus 1.03 -7.81 -6.58 -7.25 4.13 5.32 -0.20 -1.79 -6.46 -0.77 4.98 -6.00 -5.37 -9.67 3.11
Nucleus -3.90 7.31 6.62 10.47 -3.24 -3.67 2.16 8.29 11.24 2.24 -5.17 7.36 10.86 15.37 -1.45
Peroxisomes -0.92 -7.49 -0.72 0.35 7.43 1.24 -4.61 2.49 1.47 4.99 -0.34 -8.50 0.52 1.06 8.59
Vacuole 1.03 2.79 -0.95 -1.57 -2.37 -2.37 -0.91 -1.99 -0.06 -1.68 -1.25 1.68 -2.18 -1.17 -2.86
Vacuole Periphery -1.10 -0.71 -2.30 -0.89 -2.17 -2.45 3.19 -2.46 0.69 -3.10 -2.68 0.75 -3.39 0.04 -3.60
Standard name
Human Ortholog
Description Transcriptional repressor that regulates hypoxic genes during normoxia; involved in the aerobic repression of genes such as COX5b, TIR1, and HEM13; binds DNA intrastrand cross-links formed by cisplatin; HMG (high mobility group box) domain containing protein which binds and bends cisplatin-modified DNA, blocking excision repair; IXR1 has a paralog, ABF2, that arose from the whole genome duplication
Localization
Cell Percentages nucleus (100%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-2

Ixr1

Ixr1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ixr1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available