Standard name
Human Ortholog
Description Protein of unknown function; may have a role in lipid metabolism, based on localization to lipid droplets; predicted to be palmitoylated
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.91 0.97 0.94 0.93 0.89 0.78 0.74 0.74 0.54 0.6 0.42 0.5 0.97 0.98 0.99 0.89 0.87 0.82 0.64 0.74 0.47 0.77 0.78 0.69
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.14 0.05 0.19 0.09 0.05 0.07
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.11 0 0 0.16 0.11 0.33 0.44 0.4 0.59 0.6 0.71 0.71 0 0 0 0 0 0 0.12 0 0.17 0.05 0.05 0.09
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.11 0.11 0 0.09 0.05 0 0 0.07
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 2 0 0 0 0 0 0 0 0 0 0 1 0 0 4 2 6 3 4 5 2 3 2
Bud 1 2 3 0 1 2 4 4 5 4 1 3 1 1 0 1 0 1 0 2 1 1 5 0
Bud Neck 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 1 6 1 2 1 4
Bud Site 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 3 1 1 1 0 2 0 1 1 1 2 0 3 3 0 2 2 0 1 0 0 1 0
Cytoplasm 172 276 143 99 125 108 156 137 95 98 66 84 333 460 384 283 140 161 134 238 77 139 207 175
Endoplasmic Reticulum 6 9 4 2 0 5 1 4 2 4 2 1 7 3 2 7 6 4 28 15 31 16 12 16
Endosome 0 0 3 0 3 1 4 1 0 0 1 1 0 4 1 9 1 14 2 2 6 1 6 4
Golgi 0 1 0 0 1 0 0 0 0 1 0 0 0 1 0 2 0 3 0 2 2 0 0 0
Mitochondria 20 3 4 17 15 46 94 75 104 99 111 120 0 0 0 2 3 2 25 14 27 9 14 22
Nucleus 0 1 0 1 0 0 2 1 1 1 0 1 0 0 0 0 1 2 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 1 1 1 1 1 0 0 0 1 0 1 0 0 0 0 1 1 1
Nucleolus 0 0 0 0 1 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 1 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 1 0 1 0 0 0 0 0 0 0 2 0 2 1 0 0 0 1 1
Vac/Vac Membrane 2 0 1 4 4 4 6 3 7 2 2 7 3 3 0 20 17 22 5 29 8 2 7 17
Unique Cell Count 188 284 152 106 140 138 212 186 176 164 157 168 342 468 386 319 161 197 210 321 167 180 268 254
Labelled Cell Count 201 297 159 124 151 168 270 227 218 212 185 220 345 475 391 330 173 219 210 321 167 180 268 254


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.3 5.5 5.1 4.8 5.2 4.2 3.7 3.9 3.8 3.5 3.7 3.2 5.5 5.4 5.4 7.7 9.3 9.2 4.9 5.0 5.9
Std Deviation (1e-4) 0.5 1.0 0.9 1.3 2.0 1.1 1.2 1.2 1.5 2.3 1.4 0.9 1.1 1.4 1.4 1.6 2.0 1.8 1.3 1.3 1.5
Intensity Change (Log2) – – – -0.1 0.03 -0.27 -0.46 -0.39 -0.44 -0.54 -0.46 -0.66 0.1 0.08 0.09 0.59 0.87 0.85 -0.06 -0.02 0.2


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm -0.2 -1.5 -3.9 -5.0 -5.0 -8.1 -7.2 -9.8 -8.7 1.8 2.8 4.0 -1.9 -2.1 -3.4
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2.2
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 2.8 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 2.8 3.8 3.9

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration – – – – – – – – – – – – – – – – – –
Actin 0.0065 0.0003 0.0329 0.001 0.0006 0.0003 0.0244 0.0073 0.0356 0.0116 0.0029 0.009 0.0162 0.0004 0.046 0.0179 0.0233 0.0002
Bud 0.0006 0.0001 0.0007 0.0004 0.0002 0 0.0042 0.0016 0.0021 0.0046 0.0006 0.0011 0.0005 0 0.0018 0.0454 0.0011 0.0003
Bud Neck 0.0002 0.0003 0.0024 0.0002 0.0004 0.0007 0.0018 0.0001 0.0008 0.0184 0.0009 0.0024 0.0318 0 0.0003 0.0004 0.0003 0.0003
Bud Periphery 0.001 0.0001 0.001 0.0005 0.0001 0 0.0046 0.0032 0.0034 0.009 0.0009 0.002 0.0003 0 0.0026 0.0666 0.0013 0.0001
Bud Site 0.0004 0.0035 0.0327 0.0002 0.0004 0 0.0328 0.0111 0.0094 0.0084 0.0015 0.0019 0.1366 0.0005 0.0066 0.0072 0.003 0
Cell Periphery 0.0006 0.0003 0.0011 0.0002 0.0001 0.0002 0.0019 0.0004 0.0006 0.0008 0.0002 0.0002 0.0007 0.0004 0.0005 0.0008 0.0001 0.0001
Cytoplasm 0.1251 0.3278 0.3333 0.3117 0.475 0.5155 0.2915 0.4007 0.3985 0.4367 0.2851 0.4437 0.0069 0.4282 0.1245 0.2291 0.2828 0.4794
Cytoplasmic Foci 0.0525 0.0121 0.0176 0.0144 0.0333 0.0118 0.0221 0.0098 0.0298 0.0222 0.0337 0.0164 0.5627 0.0088 0.0431 0.0211 0.0273 0.0168
Eisosomes 0.0004 0.0003 0.0006 0.0002 0 0.0001 0.0007 0.0002 0.0007 0.0002 0.0001 0.0001 0.0002 0 0.0009 0.0001 0.0002 0
Endoplasmic Reticulum 0.0087 0.0142 0.0159 0.0207 0.0105 0.0234 0.0089 0.0166 0.0057 0.0203 0.0049 0.0084 0.0002 0.0192 0.008 0.0224 0.0009 0.004
Endosome 0.0244 0.0104 0.0117 0.0435 0.0465 0.0112 0.0353 0.0074 0.0241 0.0526 0.0239 0.0079 0.0302 0.0056 0.0882 0.0199 0.0194 0.0037
Golgi 0.0027 0.0013 0.0042 0.0027 0.007 0.0003 0.0054 0.0025 0.0074 0.0264 0.0247 0.0035 0.0287 0.0005 0.0311 0.0009 0.0464 0.0002
Lipid Particles 0.0117 0.0012 0.003 0.0019 0.001 0.0009 0.0152 0.0009 0.0079 0.0223 0.0024 0.0027 0.0418 0.0006 0.0595 0.0009 0.0121 0.0002
Mitochondria 0.0045 0.0004 0.004 0.002 0.0016 0.0004 0.0058 0.0007 0.0068 0.0062 0.0668 0.009 0.0018 0.0001 0.0796 0.0014 0.0881 0.0001
None 0.6866 0.6197 0.5271 0.5869 0.3421 0.4302 0.4972 0.5311 0.4407 0.3264 0.5404 0.4828 0.0014 0.532 0.4249 0.5547 0.4651 0.489
Nuclear Periphery 0.034 0.0019 0.0023 0.0048 0.0274 0.001 0.0154 0.0016 0.0029 0.0066 0.0008 0.001 0.0001 0.001 0.0007 0.0021 0.0003 0.0005
Nucleolus 0.0004 0.0001 0.0003 0.0002 0.0008 0 0.0057 0.0001 0.0004 0.0007 0.0002 0.0001 0.001 0 0.0002 0.0007 0.0001 0.0001
Nucleus 0.0056 0.0011 0.0014 0.0014 0.026 0.0011 0.0071 0.0011 0.0014 0.0039 0.0011 0.001 0.0003 0.0005 0.0003 0.002 0.0005 0.0009
Peroxisomes 0.0146 0.0002 0.002 0.0004 0.0006 0.0001 0.0047 0.0004 0.0155 0.0064 0.004 0.0038 0.1324 0 0.0767 0.0005 0.0257 0.0001
Punctate Nuclear 0.005 0.0005 0.0012 0.0009 0.0233 0.0003 0.0091 0.0005 0.0022 0.0026 0.0006 0.0011 0.0048 0.0003 0.0008 0.0021 0.0005 0.0033
Vacuole 0.0064 0.0039 0.004 0.0044 0.0023 0.0022 0.0045 0.0024 0.0033 0.011 0.0035 0.0015 0.0012 0.0016 0.0027 0.0032 0.0007 0.0006
Vacuole Periphery 0.008 0.0003 0.0004 0.0016 0.0007 0.0003 0.0016 0.0002 0.0008 0.0027 0.0007 0.0003 0.0001 0.0001 0.0009 0.0004 0.0006 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 12.0941 12.7192 9.4382 13.1664 7.8402 7.8212 18.3261 15.4068 12.3465 15.3559
Translational Efficiency 1.1359 1.5317 1.7209 0.8297 1.5199 1.771 0.9435 1.0323 1.1277 1.064

Hit Data

Dataset Hit
Protein Concentration –
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
342 282 132 1057 2128 1742 1439 60 2470 2024 1571 1117

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 682.86 770.97 951.69 1004.47 723.26 741.92 977.24 1042.45 717.67 745.97 975.09 1006.51
Standard Deviation 65.84 112.44 109.54 133.86 79.03 108.91 121.30 152.10 78.59 109.87 120.56 135.17
Intensity Change Log 2 — 0.175085 0.478902 0.556773 — 0.036749 0.434199 0.527392 — 0.105587 0.456081 0.541735

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000094 0.001631 0.001094 0.001113 0.000649 0.000512 0.000279 0.001622 0.000572 0.000668 0.000347 0.001141
Bud Neck 0.010559 0.024788 0.002947 0.006853 0.016308 0.028944 0.002922 0.005165 0.015512 0.028365 0.002924 0.006762
Bud Site 0.004651 0.026798 0.021675 0.031665 0.004998 0.018798 0.008607 0.040030 0.004950 0.019913 0.009705 0.032115
Cell Periphery 0.000249 0.000215 0.000476 0.000154 0.000135 0.000169 0.000159 0.000222 0.000151 0.000176 0.000186 0.000157
Cytoplasm 0.454795* 0.240050* 0.344833* 0.466978* 0.409550* 0.319876* 0.592305* 0.274004* 0.415815* 0.308754* 0.571512* 0.456612*
Cytoplasmic Foci 0.276305* 0.372985* 0.102547 0.094037 0.286363* 0.304789* 0.033775 0.162948 0.284970* 0.314291* 0.039553 0.097739
Eisosomes 0.000237 0.000312 0.000145 0.000047 0.000239 0.000237 0.000045 0.000161 0.000239 0.000248 0.000053 0.000053
Endoplasmic Reticulum 0.002413 0.004218 0.018652 0.005895 0.005223 0.004753 0.003657 0.007274 0.004834 0.004679 0.004917 0.005969
Endosome 0.026724 0.091306 0.025652 0.018018 0.027799 0.068063 0.005548 0.048299 0.027650 0.071301 0.007237 0.019645
Golgi 0.001479 0.012135 0.000455 0.001977 0.006191 0.013211 0.000167 0.001959 0.005539 0.013061 0.000191 0.001976
Lipid Particles 0.018812 0.018630 0.014125 0.008195 0.019755 0.017558 0.002750 0.027074 0.019624 0.017707 0.003706 0.009209
Mitochondria 0.001400 0.009203 0.000195 0.002635 0.004416 0.011701 0.000522 0.000040 0.003999 0.011353 0.000495 0.002495
Mitotic Spindle 0.000062 0.003717 0.002114 0.038319 0.002108 0.005785 0.008568 0.001773 0.001825 0.005497 0.008026 0.036356
None 0.002084 0.000316 0.000967 0.000861 0.001380 0.002171 0.002452 0.000300 0.001477 0.001913 0.002327 0.000831
Nuclear Periphery 0.000862 0.000845 0.002931 0.000788 0.000683 0.000772 0.001452 0.000406 0.000708 0.000782 0.001576 0.000767
Nuclear Periphery Foci 0.000854 0.001996 0.035668 0.008175 0.000729 0.000851 0.007182 0.024494 0.000746 0.001011 0.009575 0.009052
Nucleolus 0.001690 0.001113 0.000603 0.000424 0.000876 0.001665 0.000268 0.000127 0.000989 0.001588 0.000296 0.000408
Nucleus 0.116658 0.027015 0.124567 0.038088 0.099801 0.042734 0.128906 0.010744 0.102135 0.040544 0.128541 0.036620
Peroxisomes 0.004163 0.023100 0.001047 0.003329 0.005403 0.012534 0.000630 0.002751 0.005231 0.014006 0.000665 0.003298
Vacuole 0.074582 0.135264 0.298474* 0.269780* 0.105559 0.139018 0.199597 0.390145* 0.101270 0.138495 0.207905* 0.276245*
Vacuole Periphery 0.001329 0.004361 0.000832 0.002669 0.001835 0.005858 0.000209 0.000462 0.001765 0.005650 0.000261 0.002550

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -2.56 -6.39 -5.35 1.20 0.81 0.32 0.84 -0.79 -1.54 -2.12 -0.25 0.59 -0.83 -1.37 -3.73
Bud Neck -4.24 3.94 2.16 6.35 -4.92 -6.91 13.66 10.08 14.14 -1.31 -7.82 14.15 9.73 14.92 -6.21
Bud Site -4.72 -1.77 -5.79 0.73 -0.54 -9.43 -1.97 -1.63 -0.60 -1.47 -10.54 -2.60 -7.38 -1.21 -5.63
Cell Periphery 0.48 -2.64 1.75 3.68 9.69 -2.73 -0.44 -1.07 -0.11 -0.91 -1.64 -1.07 1.63 3.90 3.21
Cytoplasm 11.73 5.01 1.72 -11.51 -4.19 10.56 -11.43 4.58 2.00 7.54 13.80 -9.61 -0.33 -10.38 7.10
Cytoplasmic Foci -5.94 14.18 19.24 21.78 0.97 -2.60 53.30 9.44 10.49 -7.67 -4.49 56.11 37.95 39.28 -14.25
Eisosomes -1.47 4.29 10.31 5.79 9.02 0.15 18.14 2.93 2.76 -1.60 -0.44 19.20 19.61 13.27 2.67
Endoplasmic Reticulum -1.64 -4.56 -1.73 0.23 4.06 0.67 2.89 0.18 -0.00 -0.38 0.28 0.06 1.11 0.85 1.15
Endosome -7.72 0.79 3.60 10.41 3.97 -14.16 18.29 -0.95 4.14 -4.03 -16.08 17.39 8.77 21.63 -7.11
Golgi -6.28 2.80 0.88 6.62 -2.87 -7.46 13.71 5.32 10.21 -1.19 -8.90 13.96 9.97 15.34 -4.17
Lipid Particles 0.08 2.41 5.37 4.19 3.74 1.78 19.35 -0.07 -0.38 -2.57 1.66 19.52 10.96 9.02 -6.00
Mitochondria -2.66 3.57 -0.10 2.62 -2.47 -6.37 6.96 7.83 11.76 3.01 -6.90 7.21 3.82 9.38 -1.96
Mitotic Spindle -1.16 -1.38 -7.33 -4.73 -6.31 -2.62 -3.04 -1.37 -0.98 -0.83 -2.85 -3.24 -6.84 -5.55 -5.08
None 2.49 1.36 1.85 -4.18 0.78 -1.28 -1.54 4.76 3.17 4.60 -0.80 -1.18 3.31 2.29 3.46
Nuclear Periphery 0.05 -2.64 -1.18 -0.52 2.44 -0.53 -11.22 0.54 0.81 9.74 -0.49 -10.93 -3.52 -1.38 8.01
Nuclear Periphery Foci -2.45 -9.41 -9.07 -6.33 7.32 -1.27 -13.55 -3.15 -3.12 -2.03 -2.50 -15.53 -9.69 -9.29 1.15
Nucleolus 0.72 1.37 1.61 2.71 1.03 -2.35 9.96 12.98 4.66 4.81 -1.92 5.67 4.30 3.98 -1.93
Nucleus 12.07 -0.69 10.14 -5.17 6.13 18.34 -7.94 19.30 6.93 22.70 21.43 -7.60 20.23 -0.77 22.28
Peroxisomes -4.75 2.66 1.46 5.45 -3.31 -7.21 13.15 5.78 10.11 -3.60 -8.61 12.89 5.84 11.39 -4.63
Vacuole -5.77 -12.04 -27.90 -15.86 -0.86 -6.83 -22.54 -9.80 -8.86 -5.01 -8.40 -25.12 -29.35 -23.84 -6.94
Vacuole Periphery -5.06 1.90 -0.22 3.94 -1.39 -9.85 14.71 9.89 13.67 -1.43 -10.75 14.65 0.77 7.22 -2.48
Standard name
Human Ortholog
Description Protein of unknown function; may have a role in lipid metabolism, based on localization to lipid droplets; predicted to be palmitoylated
Localization
Cell Percentages cytoplasm (43%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Anr2

Anr2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Anr2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available