Standard name
Human Ortholog
Description Serine/threonine protein kinase; inhibits pheromone induced signaling downstream of MAPK, possibly at the level of the Ste12p transcription factor

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0
Cytoplasm 0.93 0.95 0.94 0.84 0.82 0.68 0.64 0.58 0.52 0.43 0.51 0.97 0.98 0.98 0.9 0.94 0.9 0.98 0.91 0.82
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0.05 0.17 0.23 0.37 0.43 0.47 0.58 0.62 0.68 0.57 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0.05 0 0.07 0.09 0.08 0.08 0 0.05 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 0 0 0
Bud 0 0 0 0 0 2 0 1 3 2 3 0 1 1 0 0 1 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1
Bud Site 0 0 0 0 0 0 0 0 0 0 1 0 0 0
Cell Periphery 1 3 0 0 1 2 2 3 2 1 5 0 0 0 0 0 0 0 0 0
Cytoplasm 190 147 67 110 130 101 83 76 63 35 57 60 131 126 27 211 129 51 70 61
Endoplasmic Reticulum 3 0 0 0 0 0 0 5 0 2 0 1 1 0 0 0 2 0 0 0
Endosome 0 0 0 2 0 1 0 0 0 0 0 0 0 0 0 2 0 0 0 1
Golgi 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0
Mitochondria 3 7 12 30 59 64 61 77 75 55 63 1 3 5 0 0 2 0 1 2
Nucleus 3 4 0 2 1 0 3 2 3 3 5 0 0 1 0 1 0 0 0 0
Nuclear Periphery 8 0 0 0 1 0 1 0 3 2 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 1 0
Vac/Vac Membrane 5 1 1 6 7 10 12 11 10 3 5 0 3 0 0 2 0 0 0 2
Unique Cell Count 205 154 71 131 159 148 129 132 121 81 111 62 134 128 31 225 144 53 77 75
Labelled Cell Count 214 163 80 150 199 180 162 175 159 103 139 62 139 133 31 225 144 53 77 75


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 6.5 5.8 5.1 5.3 5.1 4.8 4.9 4.9 4.6 4.6 4.5 5.8 5.6 5.5 4.8 5.6 6.4
Std Deviation (1e-4) 1.1 1.4 1.1 1.6 1.5 1.8 1.9 1.5 1.8 1.3 1.0 1.7 1.6 1.4 0.5 2.2 1.7
Intensity Change (Log2) -0.18 -0.1 -0.18 -0.26 -0.22 -0.23 -0.32 -0.31 -0.35 0.02 -0.03 -0.06 -0.26 -0.05 0.16


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm -0.4 -3.2 -3.8 -6.2 -6.7 -7.7 -8.4 -9.1 -8.4 0.4 1.1 1.4
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 3.1 4.6 7.1 0 8.4 10.0 0 0 0 0 0 -0.3
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 2.8 3.5 3.2 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration
Actin
Bud
Bud Neck
Bud Periphery
Bud Site
Cell Periphery
Cytoplasm
Cytoplasmic Foci
Eisosomes
Endoplasmic Reticulum
Endosome
Golgi
Lipid Particles
Mitochondria
None
Nuclear Periphery
Nucleolus
Nucleus
Peroxisomes
Punctate Nuclear
Vacuole
Vacuole Periphery

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 15.5908 10.7697 17.3934 37.8507 14.7012 13.4573 10.4088 12.9697 26.4033 21.438
Translational Efficiency 0.6842 0.7438 0.805 0.8117 0.9656 0.9246 0.8415 0.6376 0.844 0.826

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
325 126 1514 1 71 165 235 28 396 291 1749 29

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 763.61 745.11 849.41 255.99 728.62 814.79 839.50 362.53 757.34 784.62 848.08 358.86
Standard Deviation 77.19 98.44 97.64 120.90 110.91 75.26 20.80 87.70 111.19 95.00 28.21
Intensity Change Log 2 -0.035383 0.153625 -1.576749 0.161262 0.204364 -1.007066 0.063984 0.178623 -1.270578

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000128 0.000195 0.000098 0.000141 0.000247 0.000161 0.000072 0.000167 0.000149 0.000176 0.000095 0.000166
Bud Neck 0.001708 0.036373 0.004194 0.000004 0.004307 0.003102 0.003349 0.000009 0.002174 0.017508 0.004081 0.000008
Bud Site 0.002726 0.053485 0.010311 0.000016 0.024309 0.005114 0.008009 0.000038 0.006595 0.026058 0.010002 0.000037
Cell Periphery 0.000152 0.000093 0.000094 0.000139 0.000171 0.000103 0.000112 0.000810 0.000155 0.000099 0.000097 0.000786
Cytoplasm 0.653362* 0.518617* 0.734000* 0.000019 0.488058* 0.643026* 0.594699* 0.000174 0.623724* 0.589158* 0.715283* 0.000168
Cytoplasmic Foci 0.157327 0.113713 0.023273 0.000015 0.153601 0.109060 0.043401 0.000019 0.156659 0.111075 0.025977 0.000019
Eisosomes 0.000085 0.000058 0.000022 0.000874 0.000075 0.000056 0.000043 0.001204 0.000083 0.000057 0.000025 0.001193
Endoplasmic Reticulum 0.000425 0.000355 0.000912 0.000000 0.000726 0.000676 0.000773 0.000006 0.000479 0.000537 0.000893 0.000006
Endosome 0.008846 0.019375 0.002588 0.000000 0.025624 0.009286 0.005636 0.000000 0.011854 0.013655 0.002998 0.000000
Golgi 0.001720 0.001612 0.000120 0.000003 0.001450 0.002253 0.000339 0.000006 0.001671 0.001976 0.000150 0.000006
Lipid Particles 0.002282 0.003118 0.000741 0.000017 0.017808 0.003146 0.001056 0.000084 0.005066 0.003134 0.000783 0.000082
Mitochondria 0.000520 0.000257 0.000263 0.000005 0.000832 0.006665 0.000592 0.000032 0.000576 0.003890 0.000307 0.000031
Mitotic Spindle 0.000790 0.002255 0.002093 0.000197 0.043186 0.000548 0.004770 0.000321 0.008391 0.001287 0.002452 0.000316
None 0.001638 0.010046 0.004036 0.998337* 0.001555 0.001896 0.003819 0.996169* 0.001623 0.005425 0.004007 0.996244*
Nuclear Periphery 0.000227 0.000141 0.000473 0.000000 0.000249 0.000332 0.000573 0.000000 0.000231 0.000249 0.000487 0.000000
Nuclear Periphery Foci 0.001012 0.000814 0.001399 0.000001 0.001271 0.000963 0.000998 0.000001 0.001059 0.000899 0.001345 0.000001
Nucleolus 0.000371 0.001009 0.000294 0.000001 0.000346 0.000390 0.000497 0.000002 0.000366 0.000658 0.000321 0.000002
Nucleus 0.077495 0.030237 0.170106* 0.000004 0.035404 0.087652 0.222382* 0.000013 0.069948 0.062792 0.177130* 0.000013
Peroxisomes 0.001796 0.005624 0.000610 0.000228 0.008091 0.007101 0.000299 0.000942 0.002925 0.006462 0.000568 0.000918
Vacuole 0.087072 0.201486* 0.044086 0.000000 0.191292 0.117241 0.107008 0.000000 0.105758 0.153718 0.052541 0.000000
Vacuole Periphery 0.000320 0.001134 0.000286 0.000000 0.001398 0.001230 0.001573 0.000000 0.000513 0.001189 0.000459 0.000000

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin NaN NaN NaN NaN NaN 0.57 2.09 0.74 0.19 -2.15 -0.62 0.62 -0.30 0.36 -1.30
Bud Neck NaN NaN NaN NaN NaN 1.01 0.91 4.48 4.44 2.42 -3.35 -1.99 10.67 3.86 4.72
Bud Site NaN NaN NaN NaN NaN 1.83 1.59 2.37 4.20 2.50 -3.70 -1.33 3.63 5.44 6.23
Cell Periphery NaN NaN NaN NaN NaN 1.53 1.57 -2.71 -2.97 -2.97 3.90 4.37 -2.73 -2.98 -2.97
Cytoplasm NaN NaN NaN NaN NaN -3.37 -2.09 12.33 28.32 32.36* 2.33 -4.89 49.65 32.90 115.77*
Cytoplasmic Foci NaN NaN NaN NaN NaN 2.16 5.63 7.73 10.95 10.21 4.89 18.83 23.05 13.73 22.17
Eisosomes NaN NaN NaN NaN NaN 1.31 2.47 -4.61 -4.68 -4.72 4.07 11.12 -4.68 -4.79 -4.91
Endoplasmic Reticulum NaN NaN NaN NaN NaN -0.46 -1.05 3.13 4.60 8.35 -1.22 -7.54 7.87 5.71 17.65
Endosome NaN NaN NaN NaN NaN 3.52 4.53 5.87 4.34 3.82 -0.41 8.00 11.11 6.77 12.55
Golgi NaN NaN NaN NaN NaN -0.30 1.83 2.33 1.19 1.95 -0.01 1.64 1.80 1.83 5.67
Lipid Particles NaN NaN NaN NaN NaN 0.95 1.09 1.16 2.06 6.69 0.72 1.58 1.90 2.61 11.65
Mitochondria NaN NaN NaN NaN NaN -0.89 0.54 1.37 1.04 1.35 -0.88 0.92 1.86 1.07 3.80
Mitotic Spindle NaN NaN NaN NaN NaN 1.85 1.49 1.86 0.84 1.53 1.68 1.25 1.91 1.57 2.75
None NaN NaN NaN NaN NaN -0.73 -2.39 -911.13* -960.04* -980.50* -1.05 -5.05 -1078.54* -309.62* -1125.10*
Nuclear Periphery NaN NaN NaN NaN NaN -2.55 -6.80 6.56 8.40 14.46 -2.14 -11.63 10.26 9.57 28.50
Nuclear Periphery Foci NaN NaN NaN NaN NaN -0.40 -0.03 2.41 2.06 7.79 -0.41 -3.22 8.24 2.89 14.42
Nucleolus NaN NaN NaN NaN NaN -0.67 -1.05 4.86 3.26 5.89 -1.41 0.90 7.32 3.29 8.37
Nucleus NaN NaN NaN NaN NaN -5.53 -12.55 5.33 10.32 16.99 -0.01 -18.87 16.25 11.26 42.70
Peroxisomes NaN NaN NaN NaN NaN 0.44 1.78 1.62 1.18 -2.43 -0.95 2.09 1.83 1.92 -0.74
Vacuole NaN NaN NaN NaN NaN 2.44 1.92 7.59 10.68 11.94 -4.56 4.65 14.71 15.02 23.04
Vacuole Periphery NaN NaN NaN NaN NaN 0.31 0.20 2.27 1.52 1.42 -1.19 0.61 4.24 2.40 3.04
Standard name
Human Ortholog
Description Serine/threonine protein kinase; inhibits pheromone induced signaling downstream of MAPK, possibly at the level of the Ste12p transcription factor
Localization
Cell Percentages cytoplasm (73%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Prr1

Prr1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Prr1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available