Standard name
Human Ortholog
Description Protein required for transcription of rDNA by RNA polymerase I; transcription factor independent of DNA template; involved in recruitment of RNA polymerase I to rDNA; structure reveals unique HEAT repeat fold and a surface serine patch; phosphorylation of serine patch impairs cell growth and reduces RNA polymerase I binding in vitro and RNA polymerase I recruitment to the rDNA gene in vivo
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 – 0 0.06 0 0.06 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 – 0.11 0.25 0.26 0.27 – 0.14 0.21 0.08 0.12 0.12 0.27 0.36 0.16 0.11 0.21 0 0 0 0 0 0.07
Endoplasmic Reticulum 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0.2 – 0.05 0.15 0.15 0.08 – 0 0 0 0 0 0.16 0.2 0.3 0.35 0.33 0.14 0.09 0.16 0.07 0.14 0.21
Golgi 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.43 0.05 – 0.05 0 0.17 0.19 – 0.63 0.38 0.81 0.64 0.08 0 0 0.19 0.12 0.19 0 0 0 0 0 0
Nucleus 0.29 0.33 – 0.74 0.18 0.17 0.23 – 0.23 0.24 0.24 0.21 0.33 0.27 0.16 0.13 0.16 0.16 0.1 0.09 0.09 0.09 0.07 0.05
Nuclear Periphery 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.55 0.46 – 0 0 0 0 – 0 0.05 0 0 0.46 0.07 0 0.32 0.35 0.26 0.31 0.4 0.39 0.35 0.21 0.16
Peroxisomes 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0.22 0.33 – 0.26 0.25 0.2 0.29 – 0.15 0.29 0.08 0.18 0 0.37 0.32 0.34 0.35 0.29 0.36 0.35 0.2 0.37 0.36 0.26
Vac/Vac Membrane 0 0 – 0 0.13 0.15 0 – 0 0 0.07 0 0 0 0.05 0.11 0.14 0.16 0.05 0 0.06 0 0.09 0.1
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 – 0 0 2 3 – 2 6 1 4 0 0 0 1 1 0 0 0 1 0 2 0
Bud Neck 0 0 – 0 7 4 10 – 0 4 0 5 0 0 3 0 1 0 0 0 0 0 3 2
Bud Site 0 0 – 0 0 4 5 – 1 4 5 6 0 0 0 1 1 1 – – – – – –
Cell Periphery 0 0 – 0 0 0 0 – 0 1 0 0 0 1 0 0 0 1 0 0 0 0 0 0
Cytoplasm 0 6 – 2 28 33 46 – 10 28 9 19 3 34 31 19 8 12 0 0 1 2 4 9
Endoplasmic Reticulum 1 0 – 0 5 0 4 – 0 0 1 5 0 2 1 4 0 2 0 0 0 0 1 0
Endosome 2 41 – 1 17 19 13 – 2 6 4 6 1 20 17 35 26 19 11 22 7 4 26 29
Golgi 0 1 – 0 0 0 0 – 0 0 0 0 0 0 0 1 0 0 0 2 0 0 1 0
Mitochondria 30 10 – 1 4 22 33 – 46 51 92 103 2 1 0 22 9 11 0 0 0 1 4 3
Nucleus 20 68 – 14 20 22 39 – 17 33 27 33 8 34 14 15 12 9 7 23 4 5 12 7
Nuclear Periphery 1 0 – 0 0 0 0 – 0 0 0 1 0 0 0 0 0 0 0 1 0 0 0 2
Nucleolus 38 94 – 0 0 1 6 – 1 7 2 2 11 9 1 37 26 15 24 99 18 23 38 22
Peroxisomes 0 0 – 0 0 0 0 – 0 0 0 0 0 0 1 1 2 1 0 0 0 0 0 0
SpindlePole 15 68 – 5 28 26 49 – 11 39 9 29 1 46 28 39 26 17 28 88 9 24 67 36
Vac/Vac Membrane 2 2 – 0 15 19 5 – 3 4 8 3 0 4 4 13 10 9 4 3 2 0 17 13
Unique Cell Count 69 205 19 114 128 171 73 135 113 160 24 125 87 116 74 58 80 249 47 66 186 140
Labelled Cell Count 109 290 23 124 152 213 93 183 158 216 26 151 100 188 122 97 80 249 47 66 186 140


Nucleolus, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.1 5.8 – 5.6 5.7 4.5 4.7 – 3.7 4.2 3.6 3.6 5.2 5.6 5.3 7.7 7.5 7.4 – 5.5 5.5
Std Deviation (1e-4) 0.5 0.9 – 2.1 1.7 1.4 2.1 – 1.0 1.3 1.3 1.0 0.7 1.5 1.3 2.3 1.8 2.5 – 1.2 1.2
Intensity Change (Log2) – – – – – – – – – – – – – – – – – – – – –


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration -0.2497 0.0814 -0.3004 -0.7879 -0.3375 -0.4864 -0.0722 0.539 0.2617 0.0968 -0.0653 0.2046 -0.8511 -0.3276 -0.6041 -0.6234 -0.4581 -0.8544
Actin 0.0391 0 0.0041 0.0035 0.0013 0.0002 0.0145 0.0001 0.0037 0.0009 0.002 0.0002 0.0149 0.0001 0.0221 0.0001 0.0203 0.0004
Bud 0.0003 0.0001 0.0002 0.0003 0.0003 0.0001 0.0003 0.0002 0.0001 0.0003 0.0001 0 0.0007 0.0001 0.0005 0.0001 0.0003 0.0002
Bud Neck 0.0023 0.0018 0.0022 0.0015 0.0043 0.0038 0.0033 0.006 0.0034 0.0011 0.0026 0.004 0.0034 0.0026 0.0026 0.0076 0.0115 0.0054
Bud Periphery 0.0004 0.0001 0.0003 0.0005 0.0009 0.0001 0.0007 0.0003 0.0006 0.0006 0.0003 0.0001 0.0013 0.0002 0.0008 0.0002 0.0007 0.0003
Bud Site 0.005 0.0009 0.0013 0.0007 0.0017 0.0003 0.0028 0.0027 0.0018 0.0009 0.0006 0.0003 0.0089 0.0029 0.0112 0.0019 0.0017 0.0007
Cell Periphery 0.0004 0.0001 0.0002 0.0001 0.0003 0.0001 0.0004 0.0002 0.0004 0.0001 0.0001 0.0001 0.0008 0.0003 0.0003 0.0003 0.0003 0.0002
Cytoplasm 0.0075 0.0015 0.004 0.0002 0.0026 0.0004 0.0062 0.0022 0.0093 0.003 0.0005 0.0001 0.0025 0.0015 0.0008 0.0068 0.0036 0.0038
Cytoplasmic Foci 0.0269 0.0049 0.0175 0.0105 0.0141 0.0053 0.0156 0.0061 0.0092 0.0097 0.009 0.0022 0.0125 0.0045 0.0185 0.0075 0.0186 0.007
Eisosomes 0.0003 0 0.0001 0.0001 0.0001 0 0.0003 0 0.0002 0.0001 0 0 0.0004 0 0.0002 0 0.0002 0
Endoplasmic Reticulum 0.0027 0.0009 0.0006 0.0002 0.001 0.0001 0.0031 0.0001 0.0009 0.001 0.0031 0.0001 0.0018 0.0002 0.0005 0.001 0.0025 0.0003
Endosome 0.0243 0.0051 0.0127 0.0172 0.0162 0.0049 0.0319 0.0015 0.0151 0.0233 0.024 0.003 0.0158 0.0025 0.0162 0.0383 0.0326 0.0025
Golgi 0.0109 0.0004 0.0053 0.0174 0.0073 0.0032 0.0118 0.0007 0.0036 0.0039 0.0089 0.0025 0.0064 0.0007 0.0057 0.0012 0.0108 0.0015
Lipid Particles 0.0562 0.013 0.0451 0.0722 0.0753 0.0263 0.0389 0.0179 0.0286 0.0604 0.0656 0.0277 0.0169 0.0189 0.037 0.0049 0.054 0.0098
Mitochondria 0.0208 0.0001 0.019 0.084 0.0067 0.0126 0.0182 0.0002 0.0042 0.012 0.0038 0.0013 0.008 0.0004 0.0072 0.0002 0.0039 0.0005
None 0.014 0.0147 0.0038 0.0003 0.0204 0.0003 0.0083 0.0014 0.0165 0.0442 0.0006 0.0002 0.0029 0.0096 0.0008 0.001 0.0046 0.0013
Nuclear Periphery 0.0342 0.0074 0.0268 0.0048 0.0383 0.0141 0.0243 0.0149 0.0155 0.0315 0.0038 0.0018 0.0132 0.0042 0.0033 0.0032 0.0192 0.0067
Nucleolus 0.3708 0.5599 0.5948 0.458 0.4264 0.5492 0.3624 0.5521 0.5575 0.5018 0.5118 0.5189 0.3871 0.5526 0.5216 0.6195 0.3567 0.4657
Nucleus 0.1751 0.1914 0.1285 0.1304 0.1354 0.1589 0.1825 0.2305 0.2 0.1054 0.2197 0.1625 0.2028 0.2897 0.2002 0.1484 0.2358 0.1727
Peroxisomes 0.0183 0.0002 0.0058 0.0232 0.0338 0.002 0.0209 0.0009 0.005 0.0089 0.0024 0.0006 0.0084 0.0014 0.0172 0.0003 0.0053 0.0029
Punctate Nuclear 0.1853 0.1954 0.1235 0.1712 0.1883 0.2171 0.2462 0.16 0.1187 0.1621 0.1388 0.2737 0.2831 0.1048 0.128 0.1477 0.2119 0.3169
Vacuole 0.0032 0.0017 0.0022 0.0019 0.0132 0.0007 0.0057 0.0016 0.0039 0.0151 0.0011 0.0004 0.0063 0.0023 0.004 0.0092 0.0045 0.0011
Vacuole Periphery 0.0022 0.0002 0.0018 0.0018 0.0121 0.0004 0.002 0.0002 0.0018 0.0138 0.001 0.0003 0.0019 0.0003 0.0014 0.0007 0.0011 0.0003

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 19.62 19.8951 23.31 19.3632 19.7431 12.8776 19.0571 19.8294 18.1838 23.9756
Translational Efficiency 1.4423 1.4689 1.0212 1.0843 1.1827 1.8318 1.2165 0.9798 1.0112 0.9784

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1270 1043 1369 1419 1857 2443 1497 113 3127 3486 2866 1532

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 592.12 776.47 913.03 935.49 712.30 739.28 887.17 1036.09 663.49 750.41 899.52 942.91
Standard Deviation 67.17 112.78 115.94 130.99 93.23 95.89 105.37 137.02 102.36 102.66 111.30 134.05
Intensity Change Log 2 — 0.391041 0.624773 0.659833 — 0.053636 0.316726 0.540592 — 0.216623 0.464749 0.595944

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000099 0.000576 0.001088 0.000710 0.000157 0.000314 0.000758 0.000777 0.000133 0.000392 0.000916 0.000715
Bud Neck 0.002980 0.005350 0.020791 0.021051 0.003820 0.008425 0.021836 0.020013 0.003479 0.007505 0.021337 0.020974
Bud Site 0.000487 0.000936 0.006101 0.012875 0.000603 0.002082 0.004245 0.004747 0.000556 0.001739 0.005132 0.012276
Cell Periphery 0.000148 0.000078 0.000159 0.000082 0.000060 0.000065 0.000091 0.000081 0.000096 0.000069 0.000124 0.000082
Cytoplasm 0.025427 0.013242 0.033477 0.010985 0.020073 0.016532 0.032162 0.007854 0.022248 0.015548 0.032790 0.010754
Cytoplasmic Foci 0.024766 0.008161 0.002994 0.003237 0.009081 0.009350 0.001606 0.000278 0.015451 0.008994 0.002269 0.003019
Eisosomes 0.000077 0.000090 0.000086 0.000048 0.000055 0.000062 0.000068 0.000049 0.000064 0.000070 0.000076 0.000048
Endoplasmic Reticulum 0.000855 0.002322 0.005190 0.002627 0.001011 0.001409 0.004473 0.002479 0.000947 0.001682 0.004816 0.002616
Endosome 0.002324 0.003691 0.003336 0.004282 0.002633 0.005745 0.002866 0.001477 0.002507 0.005131 0.003091 0.004075
Golgi 0.001763 0.003205 0.000707 0.002641 0.001181 0.004134 0.000569 0.000730 0.001417 0.003856 0.000635 0.002500
Lipid Particles 0.009195 0.006408 0.001677 0.000434 0.003886 0.005127 0.001034 0.000403 0.006042 0.005510 0.001341 0.000432
Mitochondria 0.004008 0.007424 0.002289 0.002645 0.003856 0.012579 0.003833 0.001160 0.003918 0.011036 0.003095 0.002535
Mitotic Spindle 0.003705 0.051092 0.085215 0.262002* 0.015598 0.034329 0.090199 0.248129* 0.010768 0.039345 0.087818 0.260979*
None 0.040722 0.010618 0.007860 0.002858 0.015133 0.009792 0.009647 0.002552 0.025526 0.010039 0.008793 0.002836
Nuclear Periphery 0.000864 0.001928 0.002447 0.002486 0.001999 0.002489 0.003372 0.001469 0.001538 0.002321 0.002930 0.002411
Nuclear Periphery Foci 0.019168 0.038811 0.018033 0.014179 0.020452 0.019169 0.016128 0.009051 0.019931 0.025046 0.017038 0.013801
Nucleolus 0.618157* 0.532286* 0.303413* 0.412996* 0.547755* 0.521522* 0.335992* 0.402470* 0.576348* 0.524743* 0.320430* 0.412219*
Nucleus 0.240283* 0.304733* 0.493939* 0.228084* 0.349253* 0.338080* 0.461710* 0.289410* 0.304996* 0.328103* 0.477105* 0.232608*
Peroxisomes 0.003922 0.006369 0.002123 0.002038 0.002379 0.004892 0.001196 0.001984 0.003006 0.005334 0.001639 0.002034
Vacuole 0.000699 0.001685 0.008298 0.011462 0.000675 0.003070 0.007709 0.004293 0.000685 0.002656 0.007991 0.010933
Vacuole Periphery 0.000352 0.000994 0.000776 0.002278 0.000340 0.000832 0.000509 0.000593 0.000344 0.000881 0.000636 0.002154

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -10.93 -19.75 -17.12 -6.63 4.77 -2.49 -13.22 -4.80 -4.16 -1.75 -6.05 -23.21 -17.10 -10.11 1.62
Bud Neck -6.50 -18.30 -21.00 -19.06 -3.65 -6.85 -21.49 -6.09 -5.27 -2.09 -8.56 -27.79 -21.72 -18.23 -4.96
Bud Site -5.63 -9.11 -10.55 -10.05 -4.57 -2.76 -11.75 -3.99 -3.68 -2.65 -3.47 -14.02 -11.12 -10.02 -5.90
Cell Periphery 4.31 0.70 3.79 -0.85 3.61 -0.38 -3.76 -2.65 -2.38 -1.34 3.10 -1.66 0.22 -3.04 1.99
Cytoplasm 3.31 1.80 4.82 1.34 3.39 0.95 -0.70 2.87 2.33 3.27 2.75 0.71 5.49 3.05 4.95
Cytoplasmic Foci 7.95 10.91 11.07 6.48 1.07 1.38 8.80 9.22 10.21 2.07 7.21 13.73 13.86 11.48 1.20
Eisosomes -2.59 -2.85 7.58 9.10 9.06 -2.01 -5.21 -1.72 -1.30 -0.25 -2.01 -6.17 3.66 5.32 8.61
Endoplasmic Reticulum -9.91 -19.67 -13.66 -6.33 6.72 -4.39 -16.77 -4.24 -3.81 -0.24 -9.51 -25.82 -14.62 -10.83 5.02
Endosome -3.19 -3.53 -2.80 1.15 0.65 -3.16 -2.57 -1.24 1.08 0.10 -4.17 -3.74 -2.21 2.79 1.57
Golgi -2.04 5.23 1.43 2.86 -2.06 -3.89 2.49 1.09 4.32 -1.36 -4.15 5.36 0.31 3.80 -2.18
Lipid Particles 1.91 7.21 8.32 6.77 8.18 -1.59 6.40 5.14 6.79 -0.13 0.84 9.43 10.95 11.47 8.21
Mitochondria -2.29 4.17 3.52 3.52 -0.92 -7.01 1.29 3.45 9.45 3.45 -7.39 2.97 3.09 9.24 0.20
Mitotic Spindle -10.55 -16.23 -29.03* -20.11 -15.13 -6.14 -14.13 -8.07 -7.41 -5.51 -11.62 -21.05 -29.30 -24.88 -17.46
None 10.27 12.34 13.40 8.32 5.41 4.05 5.68 9.41 6.78 6.40 10.70 13.48 16.74 11.35 10.31
Nuclear Periphery -5.26 -14.28 -11.06 -4.66 -0.66 -2.02 -6.35 -2.00 -0.70 2.21 -4.49 -11.39 -7.93 -3.95 0.67
Nuclear Periphery Foci -5.91 -0.81 2.74 8.22 3.73 2.33 0.01 0.65 -0.15 0.65 -1.51 -0.38 4.23 5.87 4.78
Nucleolus 7.93 23.04* 20.75 12.86 -1.93 4.89 14.53 8.89 7.30 3.20 8.81 26.03* 22.95 16.70 1.52
Nucleus -6.96 -19.01 -1.92 5.14 17.35 -1.79 -5.25 2.16 2.81 4.28 -6.16 -15.78 4.13 9.50 17.76
Peroxisomes -2.02 3.47 3.90 5.29 1.11 -3.66 3.77 -0.17 3.03 -2.77 -3.72 4.88 4.15 7.27 -0.98
Vacuole -2.88 -10.32 -9.03 -8.27 -3.84 -4.55 -8.08 -3.17 -2.88 -1.89 -5.29 -12.64 -9.56 -8.48 -4.36
Vacuole Periphery -1.61 -4.23 -4.44 -0.87 -2.54 -4.69 -2.26 -2.89 -0.65 -2.31 -3.99 -4.64 -4.73 -1.80 -3.41
Standard name
Human Ortholog
Description Protein required for transcription of rDNA by RNA polymerase I; transcription factor independent of DNA template; involved in recruitment of RNA polymerase I to rDNA; structure reveals unique HEAT repeat fold and a surface serine patch; phosphorylation of serine patch impairs cell growth and reduces RNA polymerase I binding in vitro and RNA polymerase I recruitment to the rDNA gene in vivo
Localization
Cell Percentages nucleolus (40%), nucleus (22%), mixed (34%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Rrn3

Rrn3


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Rrn3-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available