Standard name | |
---|---|
Human Ortholog | |
Description | Mitochondrial ribosomal protein of the large subunit |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 1 | 0 | 0 | 1 | 0 | 2 | 4 | 0 | 2 | 10 | 16 |
Bud | 2 | 3 | 1 | 5 | 6 | 4 | 12 | 4 | 10 | 10 | 3 | 6 | 3 | 4 | 6 | 2 | 2 | 4 | 0 | 0 | 0 | 0 | 2 | 3 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 1 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 5 | 3 | 3 | 2 | 5 | 0 | 0 | 0 | 2 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 4 | 12 | 1 | 2 | 2 | 1 | 2 | 3 | 0 | 1 | 0 | 3 | 8 | 9 | 15 | 6 | 5 | 7 | 0 | 0 | 0 | 0 | 1 | 1 |
Cytoplasm | 1 | 10 | 3 | 0 | 6 | 4 | 5 | 13 | 5 | 4 | 3 | 2 | 10 | 5 | 24 | 6 | 10 | 1 | 0 | 3 | 3 | 1 | 2 | 3 |
Endoplasmic Reticulum | 0 | 1 | 1 | 1 | 0 | 3 | 1 | 1 | 1 | 0 | 4 | 1 | 4 | 3 | 3 | 2 | 2 | 1 | 1 | 0 | 0 | 0 | 1 | 3 |
Endosome | 2 | 2 | 0 | 0 | 10 | 2 | 2 | 4 | 1 | 0 | 0 | 0 | 5 | 9 | 23 | 8 | 4 | 2 | 6 | 5 | 1 | 4 | 13 | 12 |
Golgi | 4 | 25 | 0 | 0 | 6 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 22 | 25 | 72 | 19 | 27 | 20 | 9 | 19 | 6 | 6 | 44 | 55 |
Mitochondria | 207 | 324 | 58 | 147 | 213 | 157 | 309 | 316 | 289 | 256 | 169 | 203 | 274 | 412 | 461 | 9 | 3 | 4 | 186 | 329 | 58 | 96 | 212 | 236 |
Nucleus | 0 | 1 | 0 | 0 | 0 | 0 | 2 | 4 | 0 | 2 | 0 | 1 | 1 | 0 | 2 | 3 | 2 | 1 | 0 | 2 | 0 | 0 | 1 | 1 |
Nuclear Periphery | 15 | 25 | 1 | 5 | 9 | 24 | 59 | 33 | 40 | 23 | 20 | 27 | 23 | 21 | 12 | 0 | 1 | 1 | 2 | 4 | 2 | 2 | 4 | 3 |
Nucleolus | 17 | 15 | 5 | 8 | 14 | 26 | 35 | 39 | 34 | 35 | 23 | 19 | 10 | 8 | 7 | 4 | 4 | 5 | 0 | 2 | 1 | 2 | 4 | 1 |
Peroxisomes | 0 | 4 | 1 | 1 | 9 | 4 | 3 | 9 | 3 | 1 | 0 | 2 | 5 | 17 | 19 | 8 | 9 | 4 | 6 | 8 | 2 | 7 | 19 | 18 |
SpindlePole | 1 | 0 | 0 | 0 | 0 | 2 | 4 | 4 | 4 | 3 | 1 | 1 | 2 | 1 | 3 | 4 | 1 | 1 | 1 | 0 | 0 | 1 | 1 | 1 |
Vac/Vac Membrane | 39 | 54 | 3 | 1 | 11 | 5 | 8 | 20 | 11 | 4 | 2 | 3 | 39 | 26 | 44 | 13 | 18 | 13 | 7 | 15 | 0 | 3 | 8 | 29 |
Unique Cell Count | 223 | 369 | 63 | 150 | 240 | 182 | 334 | 363 | 306 | 269 | 180 | 218 | 315 | 445 | 546 | 70 | 77 | 56 | 228 | 400 | 79 | 133 | 336 | 396 |
Labelled Cell Count | 292 | 477 | 74 | 170 | 286 | 232 | 445 | 455 | 401 | 342 | 227 | 273 | 408 | 541 | 691 | 86 | 89 | 64 | 228 | 400 | 79 | 133 | 336 | 396 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 9.0 | 10.9 | 7.2 | 8.6 | 9.4 | 7.7 | 7.5 | 7.9 | 7.0 | 7.1 | 7.0 | 7.0 | 9.5 | 9.5 | 9.3 | 10.4 | 10.5 | 10.3 | 9.5 | 11.0 | 10.8 |
Std Deviation (1e-4) | 1.9 | 2.2 | 1.7 | 1.9 | 1.9 | 1.9 | 2.3 | 2.5 | 2.1 | 2.1 | 2.0 | 2.4 | 2.1 | 1.7 | 1.8 | 2.9 | 2.6 | 3.0 | 2.4 | 2.7 | 2.8 |
Intensity Change (Log2) | – | – | – | 0.25 | 0.37 | 0.1 | 0.05 | 0.13 | -0.05 | -0.02 | -0.05 | -0.04 | 0.39 | 0.39 | 0.36 | 0.52 | 0.54 | 0.51 | 0.39 | 0.61 | 0.58 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1.7 | 0 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3.1 | 4.5 | 5.2 | 5.2 |
Mitochondria | 2.1 | -0.8 | -1.2 | 0.1 | -1.1 | 0.7 | 1.0 | 0.5 | 0.3 | -1.1 | 0.1 | -1.6 | -9.1 | -10.5 | -9.3 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 2.6 | 3.3 | 2.0 | 2.6 | 0 | 2.3 | 2.5 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 1.3 | 0.6 | 0.7 | 0.7 | 1.1 | 1.0 | 0.2 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1.8 | 0 | 0 | 2.4 | 3.1 | 2.9 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 5.8482 | 5.5609 | 5.7722 | 4.759 | 4.7677 | 5.3097 | 5.3345 | 5.5864 | 5.631 | 5.3779 | 5.8939 | 5.5213 | 7.7387 | 7.2271 | 7.2078 | 7.4536 | 7.6614 | 7.2202 |
Actin | 0.0248 | 0.0027 | 0.0067 | 0.0001 | 0.0002 | 0.0063 | 0.0202 | 0.0117 | 0.0051 | 0.0085 | 0.0022 | 0.0025 | 0.012 | 0.0041 | 0.0036 | 0.0027 | 0.0004 | 0.0019 |
Bud | 0.0004 | 0.0007 | 0.002 | 0 | 0 | 0.0005 | 0.0004 | 0.0003 | 0.0001 | 0.0001 | 0 | 0.0001 | 0.0006 | 0.0039 | 0.0028 | 0.0022 | 0 | 0.0001 |
Bud Neck | 0.0033 | 0.0002 | 0.0001 | 0 | 0 | 0.0002 | 0.0004 | 0 | 0.0001 | 0 | 0 | 0.0001 | 0.0006 | 0.0002 | 0.0003 | 0 | 0 | 0.0001 |
Bud Periphery | 0.0006 | 0.0014 | 0.0043 | 0 | 0.0002 | 0.0011 | 0.0006 | 0.0004 | 0.0002 | 0.0001 | 0.0001 | 0.0002 | 0.0025 | 0.0033 | 0.0062 | 0.0196 | 0.0002 | 0.0004 |
Bud Site | 0.0024 | 0.0009 | 0.0013 | 0 | 0 | 0.0046 | 0.0044 | 0.0005 | 0.0003 | 0.0003 | 0 | 0.0001 | 0.0026 | 0.0053 | 0.002 | 0.0004 | 0 | 0.0003 |
Cell Periphery | 0.0002 | 0.0003 | 0.0001 | 0 | 0.0001 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0 | 0 | 0.0001 | 0.0003 | 0.0004 | 0.0005 | 0.0002 | 0 | 0.0001 |
Cytoplasm | 0.0005 | 0.0002 | 0.0011 | 0 | 0 | 0 | 0.0026 | 0 | 0.0002 | 0 | 0 | 0.0002 | 0.0044 | 0.002 | 0.0034 | 0.0001 | 0 | 0.0003 |
Cytoplasmic Foci | 0.0097 | 0.002 | 0.0038 | 0 | 0 | 0.0009 | 0.0158 | 0.0013 | 0.0043 | 0.0008 | 0.0008 | 0.0049 | 0.0115 | 0.0115 | 0.0055 | 0.0008 | 0.0001 | 0.0039 |
Eisosomes | 0.0003 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0006 | 0.0002 | 0.0002 | 0.0002 | 0.0002 | 0.0002 | 0.0004 | 0.0004 | 0.0002 | 0.0001 | 0.0001 | 0.0001 |
Endoplasmic Reticulum | 0.0004 | 0.001 | 0.0004 | 0 | 0 | 0.0001 | 0.0013 | 0 | 0.0003 | 0 | 0 | 0.0003 | 0.0021 | 0.001 | 0.0008 | 0.0001 | 0 | 0.0002 |
Endosome | 0.011 | 0.009 | 0.0151 | 0.0002 | 0.0001 | 0.0029 | 0.0333 | 0.0092 | 0.0127 | 0.0009 | 0.0069 | 0.0076 | 0.0192 | 0.0186 | 0.0093 | 0.0087 | 0.0002 | 0.0068 |
Golgi | 0.0262 | 0.0163 | 0.0083 | 0.0003 | 0.0002 | 0.0087 | 0.0308 | 0.0168 | 0.0137 | 0.0023 | 0.0162 | 0.0202 | 0.0223 | 0.0426 | 0.0092 | 0.0101 | 0.0003 | 0.0104 |
Lipid Particles | 0.0202 | 0.0067 | 0.0063 | 0.0002 | 0.0003 | 0.0045 | 0.0237 | 0.0009 | 0.0041 | 0.0017 | 0.0007 | 0.0075 | 0.0345 | 0.0082 | 0.0051 | 0.001 | 0.0004 | 0.0058 |
Mitochondria | 0.8516 | 0.9442 | 0.9329 | 0.9983 | 0.998 | 0.9567 | 0.8239 | 0.9525 | 0.9478 | 0.982 | 0.9658 | 0.9476 | 0.8038 | 0.8531 | 0.8275 | 0.9488 | 0.9953 | 0.948 |
None | 0.0003 | 0.0001 | 0.0009 | 0 | 0 | 0 | 0.0014 | 0 | 0.0001 | 0 | 0 | 0.0004 | 0.0367 | 0.0151 | 0.0831 | 0.0001 | 0 | 0.0075 |
Nuclear Periphery | 0.0007 | 0.0014 | 0.0006 | 0 | 0 | 0.0001 | 0.0036 | 0 | 0.0004 | 0 | 0 | 0.0013 | 0.0104 | 0.0004 | 0.0032 | 0.0002 | 0 | 0.001 |
Nucleolus | 0.0004 | 0.0001 | 0.0005 | 0 | 0 | 0.0001 | 0.0014 | 0 | 0 | 0 | 0 | 0.0001 | 0.002 | 0.0003 | 0.0021 | 0 | 0 | 0.0006 |
Nucleus | 0.0127 | 0.0002 | 0.0008 | 0 | 0 | 0 | 0.0031 | 0 | 0.0001 | 0 | 0 | 0.0001 | 0.0021 | 0.0003 | 0.0022 | 0.0001 | 0 | 0.0004 |
Peroxisomes | 0.0228 | 0.003 | 0.005 | 0.0004 | 0.0006 | 0.011 | 0.0158 | 0.004 | 0.0077 | 0.0019 | 0.0052 | 0.003 | 0.0174 | 0.0256 | 0.0184 | 0.0022 | 0.0026 | 0.0076 |
Punctate Nuclear | 0.0003 | 0.0001 | 0.0015 | 0 | 0 | 0 | 0.0035 | 0 | 0.0002 | 0 | 0 | 0.0005 | 0.0079 | 0.0009 | 0.002 | 0 | 0 | 0.0016 |
Vacuole | 0.0046 | 0.0036 | 0.0034 | 0.0001 | 0.0001 | 0.0004 | 0.0023 | 0.0006 | 0.0008 | 0.0003 | 0.0003 | 0.0005 | 0.0024 | 0.0011 | 0.0059 | 0.0007 | 0.0001 | 0.0007 |
Vacuole Periphery | 0.0065 | 0.0059 | 0.0048 | 0.0002 | 0.0002 | 0.0016 | 0.0107 | 0.0013 | 0.0016 | 0.0008 | 0.0014 | 0.0026 | 0.0044 | 0.0017 | 0.0065 | 0.0019 | 0.0003 | 0.0021 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 54.3343 | 22.4176 | 18.4832 | 39.8027 | 18.0633 | 38.9877 | 47.8514 | 54.3941 | 47.0263 | 51.8482 |
Translational Efficiency | 1.0903 | 2.7401 | 3.0859 | 1.7649 | 3.4455 | 2.5245 | 1.8191 | 2.3234 | 2.1442 | 2.0493 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✔ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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