Standard name | |
---|---|
Human Ortholog | |
Description | cAMP-dependent protein kinase catalytic subunit; promotes vegetative growth in response to nutrients via the Ras-cAMP signaling pathway; partially redundant with Tpk1p and Tpk2p; localizes to P-bodies during stationary phase; TPK3 has a paralog, TPK1, that arose from the whole genome duplication |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 2 | 2 |
Bud | 0 | 0 | 0 | 1 | 0 | 7 | 6 | 17 | 9 | 8 | 5 | 14 | 0 | 0 | 0 | 0 | 0 | 0 | 6 | 15 | 2 | 10 | 17 | 30 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 4 | 1 | 1 | 1 | 6 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 3 | 0 | 2 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 1 | 1 | 1 | 1 | 4 | 3 | 2 | 2 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 54 | 156 | 122 | 60 | 118 | 180 | 238 | 218 | 215 | 196 | 179 | 226 | 105 | 142 | 155 | 61 | 30 | 25 | 31 | 122 | 130 | 127 | 152 | 125 |
Endoplasmic Reticulum | 1 | 3 | 2 | 0 | 0 | 1 | 0 | 3 | 0 | 0 | 1 | 1 | 2 | 2 | 3 | 1 | 4 | 2 | 1 | 1 | 2 | 2 | 3 | 6 |
Endosome | 0 | 8 | 49 | 29 | 25 | 12 | 14 | 16 | 8 | 6 | 1 | 5 | 11 | 15 | 11 | 7 | 2 | 3 | 3 | 17 | 37 | 10 | 19 | 42 |
Golgi | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 2 | 0 | 1 | 2 | 2 | 8 | 11 |
Mitochondria | 0 | 3 | 12 | 16 | 51 | 123 | 236 | 301 | 230 | 210 | 218 | 304 | 1 | 1 | 0 | 4 | 2 | 2 | 0 | 1 | 2 | 3 | 6 | 11 |
Nucleus | 39 | 119 | 46 | 77 | 26 | 27 | 37 | 37 | 28 | 25 | 33 | 29 | 128 | 181 | 159 | 19 | 5 | 5 | 83 | 241 | 134 | 98 | 112 | 137 |
Nuclear Periphery | 0 | 1 | 3 | 0 | 0 | 0 | 2 | 2 | 4 | 2 | 9 | 2 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 2 | 3 | 1 | 0 | 2 |
Nucleolus | 1 | 1 | 13 | 4 | 4 | 6 | 9 | 13 | 7 | 3 | 2 | 1 | 2 | 0 | 1 | 0 | 1 | 0 | 0 | 4 | 2 | 1 | 2 | 3 |
Peroxisomes | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 3 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 4 | 5 | 1 | 0 | 1 | 4 | 3 | 1 | 1 | 3 | 0 | 1 | 1 | 0 | 1 | 1 | 2 | 16 | 16 | 7 | 12 | 24 |
Vac/Vac Membrane | 62 | 241 | 161 | 111 | 121 | 112 | 116 | 128 | 81 | 100 | 50 | 51 | 190 | 157 | 164 | 46 | 34 | 38 | 8 | 45 | 33 | 49 | 96 | 89 |
Unique Cell Count | 124 | 433 | 328 | 255 | 283 | 379 | 515 | 586 | 456 | 443 | 397 | 508 | 378 | 456 | 444 | 122 | 67 | 66 | 139 | 484 | 375 | 322 | 444 | 503 |
Labelled Cell Count | 157 | 533 | 413 | 303 | 348 | 469 | 661 | 744 | 593 | 554 | 503 | 640 | 440 | 500 | 496 | 138 | 79 | 79 | 139 | 484 | 375 | 322 | 444 | 503 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 8.3 | 7.4 | 6.5 | 6.5 | 5.7 | 5.2 | 4.3 | 4.5 | 4.4 | 4.2 | 4.2 | 4.2 | 8.4 | 7.5 | 7.8 | 8.2 | 7.8 | 9.0 | 9.0 | 8.9 | 8.6 |
Std Deviation (1e-4) | 1.5 | 1.2 | 1.3 | 1.3 | 2.1 | 1.7 | 1.6 | 1.6 | 1.6 | 1.6 | 1.2 | 1.2 | 1.2 | 1.2 | 1.3 | 1.7 | 1.3 | 2.6 | 1.3 | 1.4 | 1.3 |
Intensity Change (Log2) | – | – | – | -0.01 | -0.19 | -0.33 | -0.6 | -0.55 | -0.57 | -0.62 | -0.65 | -0.65 | 0.36 | 0.21 | 0.26 | 0.33 | 0.26 | 0.46 | 0.47 | 0.44 | 0.4 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | -3.5 | 1.1 | 2.8 | 2.6 | 0 | 2.8 | 2.0 | 2.1 | 2.1 | -2.7 | -1.8 | -0.7 | 2.5 | 1.2 | 0.1 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | -1.3 | -2.3 | -5.6 | 0 | 0 | 0 | 0 | 0 | 0 | -5.7 | -5.9 | -6.4 | -2.6 | -2.7 | -2.3 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 1.5 | 5.8 | 9.7 | 0 | 0 | 0 | 0 | 0 | 0 | -3.3 | -3.7 | -4.1 | 0 | 0 | 0 |
Nucleus | 4.7 | -1.8 | -3.0 | -3.2 | -3.9 | -3.7 | -4.0 | -2.5 | -4.1 | 6.1 | 7.8 | 6.8 | 0.4 | -1.5 | -1.4 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | -1.7 | -1.9 | -2.0 | 0 | 0 | 0 | 0 | 0 | 0 | -3.2 | -4.3 | -3.8 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | -1.3 | -1.6 | -5.3 | -8.0 | -8.5 | -9.4 | -7.7 | -10.8 | -12.7 | 0.3 | -4.1 | -3.4 | -2.2 | 0.2 | 1.3 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 3.6118 | 4.7144 | 4.1889 | 3.9065 | 3.6153 | 4.2364 | 6.5343 | 7.9964 | 7.0618 | 6.6622 | 6.3267 | 7.1669 | 2.9078 | 3.2923 | 2.6225 | 3.3011 | 2.4054 | 2.8259 |
Actin | 0.0017 | 0.0003 | 0.0014 | 0.0011 | 0 | 0.0028 | 0.0208 | 0.0001 | 0.006 | 0.0075 | 0.0135 | 0.0021 | 0.0062 | 0.0068 | 0.0107 | 0.0045 | 0.0066 | 0.0044 |
Bud | 0.0003 | 0.0004 | 0.0004 | 0.0001 | 0 | 0.0003 | 0.0006 | 0.0001 | 0.0003 | 0.0015 | 0.0002 | 0.0003 | 0.0016 | 0.0015 | 0.0015 | 0.0005 | 0.001 | 0.0011 |
Bud Neck | 0.0013 | 0.0007 | 0.001 | 0.0012 | 0.0004 | 0.0022 | 0.0077 | 0.0004 | 0.0006 | 0.0217 | 0.0009 | 0.0021 | 0.0032 | 0.0025 | 0.0043 | 0.0013 | 0.0021 | 0.0063 |
Bud Periphery | 0.0004 | 0.0003 | 0.0006 | 0.0001 | 0 | 0.0002 | 0.0008 | 0 | 0.0006 | 0.0017 | 0.0004 | 0.0004 | 0.0013 | 0.0013 | 0.0015 | 0.0005 | 0.0013 | 0.001 |
Bud Site | 0.0012 | 0.0024 | 0.0015 | 0.0004 | 0.0001 | 0.0004 | 0.0027 | 0.0003 | 0.0013 | 0.0303 | 0.0003 | 0.0002 | 0.0055 | 0.0071 | 0.0061 | 0.0013 | 0.0018 | 0.0022 |
Cell Periphery | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0 | 0 | 0.0005 | 0 | 0.0001 | 0.0013 | 0 | 0 | 0.0001 | 0.0001 | 0.0001 | 0 | 0.0001 | 0.0001 |
Cytoplasm | 0.0627 | 0.082 | 0.0681 | 0.0344 | 0.0179 | 0.0545 | 0.1319 | 0.1584 | 0.0744 | 0.0384 | 0.0793 | 0.0818 | 0.0164 | 0.0381 | 0.0121 | 0.0131 | 0.0121 | 0.0202 |
Cytoplasmic Foci | 0.0205 | 0.0007 | 0.0086 | 0.0047 | 0.0003 | 0.0047 | 0.0074 | 0.0008 | 0.0018 | 0.004 | 0.0081 | 0.0007 | 0.0666 | 0.0804 | 0.0618 | 0.0316 | 0.0601 | 0.0517 |
Eisosomes | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0.0001 | 0 | 0.0001 | 0.0004 | 0.0002 | 0 | 0 | 0.0002 | 0 | 0 | 0.0001 | 0 |
Endoplasmic Reticulum | 0.0041 | 0.0009 | 0.0013 | 0.0034 | 0.0005 | 0.001 | 0.0066 | 0.0007 | 0.0034 | 0.0021 | 0.0016 | 0.0021 | 0.0006 | 0.0012 | 0.0005 | 0.0002 | 0.0002 | 0.0006 |
Endosome | 0.0475 | 0.001 | 0.0086 | 0.0029 | 0.002 | 0.0071 | 0.0126 | 0.0015 | 0.004 | 0.009 | 0.0085 | 0.0033 | 0.0178 | 0.0124 | 0.0081 | 0.0079 | 0.0157 | 0.0086 |
Golgi | 0.0066 | 0.0001 | 0.0048 | 0.0023 | 0 | 0.0048 | 0.0029 | 0.0001 | 0.0007 | 0.0053 | 0.004 | 0.0003 | 0.0122 | 0.0084 | 0.0083 | 0.0058 | 0.0115 | 0.0065 |
Lipid Particles | 0.0182 | 0.0003 | 0.0044 | 0.0082 | 0.0001 | 0.0081 | 0.0082 | 0 | 0.0019 | 0.0043 | 0.0272 | 0.0003 | 0.0331 | 0.0186 | 0.0151 | 0.0041 | 0.0329 | 0.0259 |
Mitochondria | 0.0116 | 0.0058 | 0.0209 | 0.0007 | 0.0002 | 0.005 | 0.0021 | 0.0002 | 0.0014 | 0.0189 | 0.0026 | 0.0014 | 0.0093 | 0.0052 | 0.0085 | 0.0277 | 0.0094 | 0.0054 |
None | 0.0217 | 0.0025 | 0.0015 | 0.0012 | 0.0055 | 0.0096 | 0.0032 | 0.0005 | 0.0048 | 0.0163 | 0.0049 | 0.002 | 0.0036 | 0.0106 | 0.0026 | 0.0023 | 0.0057 | 0.0012 |
Nuclear Periphery | 0.0263 | 0.0106 | 0.0112 | 0.0074 | 0.0139 | 0.009 | 0.0235 | 0.0079 | 0.0102 | 0.0068 | 0.0128 | 0.0155 | 0.0042 | 0.0037 | 0.0023 | 0.0022 | 0.0013 | 0.0035 |
Nucleolus | 0.0022 | 0.0017 | 0.0017 | 0.0019 | 0.0025 | 0.0025 | 0.0049 | 0.0008 | 0.0014 | 0.0037 | 0.0013 | 0.002 | 0.0046 | 0.0017 | 0.0044 | 0.0042 | 0.0078 | 0.0031 |
Nucleus | 0.7427 | 0.8761 | 0.8483 | 0.8979 | 0.9493 | 0.8719 | 0.7388 | 0.8247 | 0.8778 | 0.8122 | 0.8116 | 0.8777 | 0.1593 | 0.148 | 0.1468 | 0.2408 | 0.1399 | 0.1677 |
Peroxisomes | 0.0114 | 0.0002 | 0.0046 | 0.0268 | 0 | 0.0031 | 0.0108 | 0 | 0.0006 | 0.0031 | 0.0142 | 0.0002 | 0.6283 | 0.6231 | 0.6796 | 0.6349 | 0.678 | 0.6664 |
Punctate Nuclear | 0.0087 | 0.0122 | 0.0074 | 0.003 | 0.0059 | 0.0114 | 0.0097 | 0.0024 | 0.0067 | 0.0079 | 0.007 | 0.0061 | 0.0238 | 0.0279 | 0.0246 | 0.0154 | 0.0117 | 0.0231 |
Vacuole | 0.0075 | 0.0015 | 0.0028 | 0.002 | 0.001 | 0.001 | 0.0032 | 0.001 | 0.0015 | 0.0025 | 0.0007 | 0.001 | 0.0018 | 0.0008 | 0.0009 | 0.0012 | 0.0005 | 0.0009 |
Vacuole Periphery | 0.0031 | 0.0003 | 0.0007 | 0.0002 | 0.0003 | 0.0004 | 0.0008 | 0.0001 | 0.0004 | 0.0012 | 0.0006 | 0.0004 | 0.0005 | 0.0003 | 0.0002 | 0.0004 | 0.0002 | 0.0003 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 11.9835 | 23.8972 | 11.618 | 20.8697 | 20.3178 | 13.8195 | 18.1218 | 20.2444 | 13.356 | 21.2694 |
Translational Efficiency | 2.3537 | 1.1305 | 1.9766 | 1.0888 | 1.1337 | 1.8254 | 1.3999 | 1.249 | 1.7238 | 1.2655 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
1262 | 1827 | 154 | 1277 | 1699 | 937 | 1296 | 1902 | 2961 | 2764 | 1450 | 3179 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 749.80 | 880.38 | 1107.23 | 1048.20 | 808.65 | 881.58 | 1023.12 | 1103.49 | 783.57 | 880.79 | 1032.05 | 1081.28 |
Standard Deviation | 97.43 | 148.15 | 128.78 | 142.45 | 128.78 | 120.86 | 131.60 | 149.38 | 120.04 | 139.50 | 133.84 | 149.12 |
Intensity Change Log 2 | — | 0.231621 | 0.562377 | 0.483336 | — | 0.124576 | 0.339388 | 0.448486 | — | 0.177069 | 0.450979 | 0.465358 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000306 | 0.000153 | 0.001385 | 0.000737 | 0.000094 | 0.000536 | 0.000567 | 0.001172 | 0.000184 | 0.000283 | 0.000654 | 0.000997 |
Bud Neck | 0.003029 | 0.003882 | 0.003740 | 0.016304 | 0.001771 | 0.005938 | 0.008031 | 0.011885 | 0.002307 | 0.004579 | 0.007575 | 0.013660 |
Bud Site | 0.005530 | 0.001822 | 0.002664 | 0.026795 | 0.001091 | 0.003707 | 0.005041 | 0.024337 | 0.002983 | 0.002461 | 0.004789 | 0.025325 |
Cell Periphery | 0.000305 | 0.000103 | 0.000208 | 0.000156 | 0.000082 | 0.000133 | 0.000110 | 0.000110 | 0.000177 | 0.000113 | 0.000121 | 0.000128 |
Cytoplasm | 0.323464* | 0.298572* | 0.089916 | 0.169270* | 0.346688* | 0.321935* | 0.096562 | 0.211913* | 0.336790* | 0.306492* | 0.095856 | 0.194783* |
Cytoplasmic Foci | 0.029950 | 0.005771 | 0.002284 | 0.013297 | 0.019576 | 0.019412 | 0.000682 | 0.009598 | 0.023998 | 0.010396 | 0.000852 | 0.011083 |
Eisosomes | 0.000035 | 0.000010 | 0.000073 | 0.000025 | 0.000020 | 0.000034 | 0.000017 | 0.000022 | 0.000027 | 0.000018 | 0.000023 | 0.000023 |
Endoplasmic Reticulum | 0.001877 | 0.002163 | 0.014244 | 0.003768 | 0.001622 | 0.004109 | 0.004763 | 0.002938 | 0.001731 | 0.002823 | 0.005770 | 0.003271 |
Endosome | 0.001428 | 0.001233 | 0.002589 | 0.008969 | 0.001156 | 0.003983 | 0.000480 | 0.006568 | 0.001272 | 0.002165 | 0.000704 | 0.007533 |
Golgi | 0.000570 | 0.000665 | 0.000140 | 0.008207 | 0.000145 | 0.000980 | 0.000077 | 0.004269 | 0.000326 | 0.000772 | 0.000083 | 0.005851 |
Lipid Particles | 0.002559 | 0.000392 | 0.001803 | 0.001494 | 0.001286 | 0.001814 | 0.001023 | 0.000828 | 0.001828 | 0.000874 | 0.001106 | 0.001096 |
Mitochondria | 0.002967 | 0.001196 | 0.000941 | 0.002332 | 0.000887 | 0.003419 | 0.001151 | 0.003500 | 0.001773 | 0.001949 | 0.001129 | 0.003031 |
Mitotic Spindle | 0.001925 | 0.003250 | 0.000622 | 0.306952* | 0.002310 | 0.010130 | 0.003400 | 0.225845* | 0.002146 | 0.005582 | 0.003105 | 0.258426* |
None | 0.007696 | 0.010022 | 0.002869 | 0.007147 | 0.010199 | 0.006038 | 0.005091 | 0.009042 | 0.009132 | 0.008672 | 0.004855 | 0.008281 |
Nuclear Periphery | 0.000825 | 0.001169 | 0.001933 | 0.004411 | 0.001017 | 0.001477 | 0.001569 | 0.002708 | 0.000935 | 0.001273 | 0.001607 | 0.003392 |
Nuclear Periphery Foci | 0.000885 | 0.000710 | 0.002575 | 0.007838 | 0.000764 | 0.001134 | 0.000772 | 0.002773 | 0.000816 | 0.000854 | 0.000963 | 0.004807 |
Nucleolus | 0.002009 | 0.001208 | 0.000647 | 0.003483 | 0.001617 | 0.001344 | 0.000503 | 0.001623 | 0.001784 | 0.001254 | 0.000518 | 0.002370 |
Nucleus | 0.600232* | 0.658675* | 0.799491* | 0.359647* | 0.598117* | 0.580360* | 0.858179* | 0.437266* | 0.599018* | 0.632126* | 0.851946* | 0.406087* |
Peroxisomes | 0.001524 | 0.000291 | 0.000250 | 0.004021 | 0.000396 | 0.001272 | 0.000231 | 0.003670 | 0.000876 | 0.000624 | 0.000233 | 0.003811 |
Vacuole | 0.012711 | 0.007708 | 0.071411 | 0.047976 | 0.010913 | 0.031195 | 0.011674 | 0.037292 | 0.011680 | 0.015670 | 0.018018 | 0.041584 |
Vacuole Periphery | 0.000171 | 0.001004 | 0.000217 | 0.007173 | 0.000248 | 0.001050 | 0.000078 | 0.002643 | 0.000215 | 0.001019 | 0.000093 | 0.004463 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | 1.12 | -7.60 | -6.20 | -16.36 | 4.45 | -2.85 | -13.69 | -9.18 | -1.05 | -0.02 | -1.76 | -10.66 | -10.46 | -7.35 | 0.13 |
Bud Neck | -1.33 | -2.32 | -13.08 | -13.48 | -12.73 | -4.17 | -6.69 | -17.53 | -10.71 | -8.03 | -4.67 | -6.29 | -21.69 | -18.38 | -10.14 |
Bud Site | 2.12 | 0.75 | -8.19 | -12.25 | -11.08 | -4.95 | -3.51 | -13.69 | -11.21 | -9.70 | 0.33 | -1.50 | -15.11 | -16.95 | -12.81 |
Cell Periphery | 1.69 | 0.50 | 1.25 | -3.46 | 4.07 | -4.00 | -5.82 | -4.68 | 2.15 | 2.66 | 1.23 | 0.75 | 0.99 | -1.25 | 1.69 |
Cytoplasm | 2.92 | 18.50 | 23.11 | 22.73 | -2.36 | 3.10 | 33.47 | 23.55 | 16.00 | -12.29 | 4.99 | 40.78 | 32.59 | 26.53 | -11.45 |
Cytoplasmic Foci | 11.83 | 13.43 | 10.16 | -2.13 | -5.27 | 0.42 | 15.23 | 9.58 | 6.98 | -9.23 | 10.69 | 20.82 | 13.97 | 3.73 | -11.74 |
Eisosomes | 10.72 | -6.64 | -4.45 | -19.09 | 5.19 | -5.83 | -1.57 | -9.34 | 0.08 | -7.38 | 4.49 | -1.96 | -9.28 | -14.70 | -5.68 |
Endoplasmic Reticulum | -2.52 | -11.54 | -13.14 | -10.94 | 8.30 | -7.45 | -13.07 | -11.41 | 1.62 | 5.95 | -7.51 | -16.01 | -17.21 | -6.64 | 7.06 |
Endosome | 0.83 | -4.30 | -8.25 | -6.93 | -2.67 | -4.34 | 4.30 | -7.01 | 0.94 | -8.91 | -2.30 | 3.01 | -10.70 | -4.63 | -12.09 |
Golgi | -0.12 | 1.66 | -1.96 | -1.62 | -2.83 | -2.58 | 1.42 | -3.45 | -0.71 | -3.65 | -1.26 | 2.22 | -3.58 | -1.70 | -4.59 |
Lipid Particles | 3.25 | 0.49 | 0.85 | -12.98 | 0.78 | -2.08 | 1.15 | 1.04 | 2.86 | -0.81 | 3.17 | 1.87 | 1.16 | -6.06 | -1.43 |
Mitochondria | 1.58 | 1.87 | 0.40 | -2.84 | -9.59 | -2.33 | -2.96 | -6.11 | 0.54 | -5.39 | -0.10 | 0.67 | -1.73 | -1.63 | -8.00 |
Mitotic Spindle | -1.05 | 1.19 | -25.78* | -25.63* | -25.94* | -2.80 | -3.35 | -28.06* | -26.24* | -26.60* | -2.65 | -3.50 | -38.04 | -37.26* | -35.94* |
None | -1.98 | 6.97 | -2.81 | -0.44 | -10.97 | 2.96 | 4.65 | -3.29 | -6.91 | -14.43 | 0.83 | 6.12 | -4.33 | -5.53 | -16.40 |
Nuclear Periphery | -3.66 | -10.02 | -15.73 | -14.69 | -9.05 | -4.35 | -9.56 | -18.03 | -14.09 | -9.77 | -4.90 | -12.17 | -23.72 | -21.41 | -13.25 |
Nuclear Periphery Foci | 1.00 | -5.12 | -8.70 | -8.81 | -6.61 | -2.75 | -1.97 | -7.32 | -6.51 | -5.06 | -0.61 | -2.60 | -11.24 | -11.10 | -8.66 |
Nucleolus | 1.43 | 2.54 | -8.10 | -9.50 | -10.16 | 0.35 | 2.37 | -10.75 | -10.69 | -11.85 | 1.47 | 3.19 | -13.41 | -14.41 | -15.29 |
Nucleus | -5.63 | -5.54 | 16.00 | 22.80 | 14.62 | 1.38 | -27.00 | 14.54 | 11.09 | 42.92* | -4.48 | -30.14 | 21.36 | 25.62 | 51.06 |
Peroxisomes | 2.46 | 2.54 | -1.22 | -4.70 | -4.84 | -2.89 | 1.73 | -3.56 | -1.09 | -4.12 | 1.16 | 2.90 | -3.11 | -4.61 | -6.06 |
Vacuole | 2.96 | -8.17 | -17.25 | -18.21 | 2.26 | -8.78 | -5.53 | -17.23 | -6.25 | -12.86 | -5.30 | -8.96 | -24.29 | -20.30 | -12.89 |
Vacuole Periphery | -1.36 | -2.49 | -2.91 | -0.43 | -2.70 | -2.08 | 2.55 | -1.81 | 1.00 | -2.78 | -1.87 | 2.86 | -3.43 | 0.25 | -4.06 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
---|
Gene | Images |
---|