ORF
Human Ortholog
Description Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified gene MRPL38
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0.12 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.1
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.87 0.79 0.76 0.79 0.87 0.83 – 0.78 0.67 0.63 0.64 0.63 0.79 0.91 0.96 0.56 0.57 0.52 0.79 0.79 0.76 0.77 0.77 0.54
Endoplasmic Reticulum 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0.06 0 0.08 0 0 0 0 0 0.07
Golgi 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0.07 0 0 0 0 0 0.05
Mitochondria 0 0 0.18 0.17 0.07 0.33 – 0.41 0.6 0.55 0.52 0.58 0 0 0 0 0.06 0 0 0 0.05 0 0 0
Nucleus 0 0 0.06 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0.07 0.06 0 0.06 0 0
Nuclear Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0.13 0 0.09 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0.07
Vac/Vac Membrane 0.12 0.17 0 0.11 0.09 0 – 0 0 0 0 0 0.23 0.08 0 0.31 0.32 0.3 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 – 0 0 0 1 1 0 0 0 0 0 1 0 0 0 0 2 7
Bud 0 0 2 4 3 4 – 7 7 8 4 6 2 1 2 0 0 0 5 3 1 4 21 42
Bud Neck 0 0 0 0 0 0 – 2 0 3 0 0 0 0 0 0 0 0 2 0 0 0 1 4
Bud Site 0 0 0 0 0 0 – 1 0 0 0 1 0 0 0 0 0 0 – – – – – –
Cell Periphery 1 0 0 0 0 1 – 2 1 2 4 2 0 0 1 1 0 0 0 0 0 0 0 0
Cytoplasm 227 46 13 120 215 192 – 406 183 200 112 162 261 429 519 53 39 47 210 76 46 79 282 241
Endoplasmic Reticulum 2 0 0 0 2 0 – 0 0 1 0 3 1 1 1 1 1 4 0 0 0 0 1 3
Endosome 2 1 0 1 3 1 – 2 0 2 1 1 8 5 4 6 3 7 6 1 1 0 8 29
Golgi 1 0 0 1 0 0 – 0 0 1 0 0 1 0 0 3 2 6 3 0 0 0 2 22
Mitochondria 7 2 3 26 17 77 – 213 163 176 90 148 4 12 6 4 4 4 8 1 2 0 3 11
Nucleus 3 0 1 0 1 0 – 6 0 8 3 3 3 0 1 1 0 0 18 5 1 6 13 17
Nuclear Periphery 0 0 0 1 0 0 – 3 1 1 0 2 0 0 0 0 0 0 1 0 0 0 0 1
Nucleolus 1 1 0 0 0 0 – 2 0 2 1 0 2 5 1 12 3 8 0 0 0 1 1 1
Peroxisomes 0 0 0 0 0 0 – 0 0 0 0 0 0 3 1 1 1 0 0 1 0 0 0 5
SpindlePole 0 0 0 0 0 0 – 5 2 1 1 2 1 1 1 1 0 1 3 1 1 6 10 30
Vac/Vac Membrane 32 10 0 17 23 2 – 16 3 5 7 3 75 37 15 29 22 27 5 2 2 4 13 19
Unique Cell Count 260 58 17 152 248 231 521 272 319 174 256 332 474 538 95 68 90 268 96 61 103 369 444
Labelled Cell Count 276 60 19 170 264 277 665 360 410 224 334 358 494 552 112 75 105 268 96 61 103 369 444


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 7.2 6.8 4.9 6.1 5.7 3.5 – 3.5 3.0 3.5 3.2 3.6 10.6 9.1 6.6 18.9 16.6 17.7 7.2 7.2 7.5
Std Deviation (1e-4) 1.7 2.0 1.5 3.3 1.8 1.2 – 1.4 1.3 1.8 1.6 8.6 3.6 3.9 1.8 13.9 7.5 9.2 3.4 2.2 2.3
Intensity Change (Log2) – – – 0.32 0.22 -0.47 – -0.48 -0.72 -0.48 -0.59 -0.44 1.11 0.9 0.44 1.96 1.77 1.86 0.56 0.55 0.62


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.2 1.2 0.7 – 0.1 -0.8 -1.1 -1.0 -1.1 0.2 1.9 4.1 -1.6 -1.4 -1.8
Endoplasmic Reticulum 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 3.9248 7.4421 6.3978 5.7154 5.1401 6.6306 5.196 9.7457 7.5961 6.5702 7.8193 7.5345 7.3681 10.7155 9.8428 8.428 8.9045 9.0336
Actin 0.0308 0.0026 0.0264 0.0573 0.0059 0.0072 0.037 0.0004 0.0088 0.0176 0.0069 0.0021 0.0622 0.0005 0.003 0.0084 0.0163 0.0024
Bud 0.001 0.0009 0.0016 0.0011 0.0008 0.0004 0.0016 0.0003 0.0006 0.0005 0.0018 0.0003 0.0006 0.0002 0.0003 0.0022 0.0004 0.0003
Bud Neck 0.0054 0.0005 0.0018 0.0097 0.0012 0.0017 0.0075 0.0004 0.0016 0.0252 0.0018 0.0023 0.0033 0.0003 0.0008 0.0127 0.001 0.0017
Bud Periphery 0.0006 0.0004 0.0009 0.0029 0.001 0.0005 0.0014 0.0002 0.0006 0.0003 0.0005 0.0002 0.0004 0 0.0001 0.0032 0.0006 0.0002
Bud Site 0.0065 0.0014 0.0022 0.0025 0.0038 0.0004 0.0075 0.0005 0.0018 0.0009 0.0008 0.0005 0.0008 0.0004 0.0037 0.003 0.0006 0.0002
Cell Periphery 0.0004 0.0001 0.0001 0.0017 0.0001 0 0.001 0 0.0001 0.0001 0.0001 0 0.0001 0 0.0001 0.0003 0.0002 0
Cytoplasm 0.6952 0.844 0.6719 0.6221 0.6868 0.6947 0.6143 0.7499 0.6688 0.6007 0.6543 0.6269 0.6828 0.8004 0.7385 0.6927 0.7231 0.7022
Cytoplasmic Foci 0.0378 0.0036 0.0169 0.0166 0.0178 0.019 0.0381 0.0123 0.0271 0.0163 0.0539 0.0148 0.0077 0.0022 0.0054 0.0022 0.0038 0.0032
Eisosomes 0.0003 0.0001 0.0001 0.0005 0.0001 0.0001 0.0007 0 0 0.0001 0 0 0.0003 0 0.0002 0 0.0004 0
Endoplasmic Reticulum 0.0143 0.0029 0.0057 0.0043 0.0039 0.0054 0.0122 0.0026 0.0046 0.0042 0.0058 0.0035 0.0116 0.0024 0.0027 0.0023 0.0142 0.003
Endosome 0.0539 0.0034 0.0273 0.0177 0.0349 0.0172 0.0539 0.0034 0.0227 0.0332 0.0295 0.0149 0.0187 0.0011 0.0025 0.004 0.0109 0.0044
Golgi 0.0083 0.0006 0.0154 0.0212 0.011 0.0047 0.01 0.0004 0.0069 0.0221 0.002 0.0033 0.0042 0.0001 0.0003 0.0018 0.0024 0.0048
Lipid Particles 0.0107 0.0002 0.0086 0.0087 0.0071 0.0051 0.0094 0.0002 0.007 0.0108 0.0034 0.0024 0.003 0 0.001 0.0003 0.0012 0.0044
Mitochondria 0.0023 0.0089 0.0074 0.0195 0.0551 0.0128 0.0074 0.0063 0.0092 0.0111 0.0017 0.0074 0.0021 0.0002 0.0013 0.0011 0.0083 0.0067
None 0.0329 0.0038 0.0082 0.0088 0.0093 0.0049 0.0184 0.0114 0.003 0.0026 0.0068 0.002 0.0339 0.0023 0.0111 0.0013 0.0034 0.0017
Nuclear Periphery 0.0202 0.008 0.0142 0.0104 0.0129 0.0174 0.0321 0.0129 0.0154 0.0153 0.0196 0.0175 0.0247 0.0095 0.0101 0.0117 0.0229 0.0153
Nucleolus 0.0007 0.0001 0.0006 0.0004 0.0005 0.0005 0.0008 0.0001 0.0004 0.0007 0.0005 0.0002 0.0004 0.0001 0.0004 0.0003 0.0003 0.0004
Nucleus 0.0574 0.113 0.1732 0.1717 0.0856 0.1945 0.1199 0.185 0.1995 0.2179 0.1867 0.2907 0.1308 0.1779 0.2029 0.2461 0.1813 0.2406
Peroxisomes 0.0045 0.0002 0.006 0.0083 0.0508 0.0024 0.007 0.0004 0.0073 0.0128 0.0051 0.0012 0.002 0.0001 0.0014 0.0004 0.0003 0.0008
Punctate Nuclear 0.0094 0.0035 0.0079 0.0045 0.0069 0.0062 0.0106 0.0118 0.0083 0.004 0.0154 0.007 0.0065 0.0014 0.0131 0.003 0.0047 0.0062
Vacuole 0.006 0.0014 0.0026 0.007 0.0029 0.0034 0.0067 0.0011 0.0048 0.0028 0.0026 0.0021 0.0024 0.0008 0.0007 0.0024 0.0021 0.001
Vacuole Periphery 0.0013 0.0005 0.001 0.003 0.0014 0.0015 0.0024 0.0003 0.0013 0.0007 0.0008 0.0005 0.0013 0.0002 0.0002 0.0005 0.0017 0.0005

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression – – – – – – – – – –
Translational Efficiency – – – – – – – – – –

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression –
Translational Efficiency –

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1761 1502 1871 1338 940 1353 1634 1208 2701 2855 3505 2546

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 733.79 957.90 1480.96 2856.67 625.44 953.10 2321.38 2893.21 696.08 955.63 1872.76 2874.01
Standard Deviation 112.59 178.65 311.92 868.43 110.52 164.41 652.44 851.95 123.20 172.07 652.80 860.85
Intensity Change Log 2 — 0.384508 1.013094 1.960895 — 0.607756 1.892039 2.209728 — 0.491538 1.484098 2.080742

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000123 0.000322 0.000550 0.000715 0.000330 0.000423 0.001114 0.000444 0.000195 0.000370 0.000813 0.000586
Bud Neck 0.004862 0.007303 0.009453 0.019033 0.010512 0.008825 0.013244 0.019331 0.006829 0.008024 0.011220 0.019174
Bud Site 0.002703 0.008765 0.010474 0.034240 0.006376 0.012554 0.020348 0.037818 0.003981 0.010561 0.015077 0.035938
Cell Periphery 0.000122 0.000118 0.000166 0.000435 0.000244 0.000139 0.000516 0.000480 0.000165 0.000128 0.000330 0.000457
Cytoplasm 0.580454* 0.611128* 0.337562* 0.036677 0.440873* 0.580971* 0.193129* 0.042842 0.531877* 0.596837* 0.270228* 0.039602
Cytoplasmic Foci 0.131655 0.051760 0.014257 0.302401* 0.211268* 0.106225 0.113858 0.269950* 0.159362 0.077571 0.060690 0.287004*
Eisosomes 0.000049 0.000021 0.000012 0.000099 0.000119 0.000040 0.000102 0.000062 0.000074 0.000030 0.000054 0.000082
Endoplasmic Reticulum 0.002098 0.003053 0.001556 0.000640 0.003173 0.002388 0.000309 0.000227 0.002472 0.002738 0.000975 0.000444
Endosome 0.005124 0.004862 0.001161 0.004462 0.012206 0.011500 0.002565 0.003881 0.007589 0.008008 0.001816 0.004187
Golgi 0.001227 0.001410 0.000403 0.005662 0.003265 0.003354 0.002446 0.006592 0.001937 0.002331 0.001355 0.006103
Lipid Particles 0.005396 0.001697 0.000985 0.002828 0.014893 0.003241 0.008227 0.002847 0.008701 0.002429 0.004361 0.002837
Mitochondria 0.003323 0.002529 0.001597 0.002585 0.009864 0.002022 0.005556 0.002966 0.005599 0.002289 0.003443 0.002766
Mitotic Spindle 0.001148 0.007098 0.036946 0.483033* 0.001980 0.025380 0.241879* 0.488272* 0.001438 0.015762 0.132484* 0.485519*
None 0.010170 0.007816 0.009052 0.001646 0.023705 0.004214 0.008529 0.003070 0.014881 0.006109 0.008808 0.002322
Nuclear Periphery 0.000754 0.001103 0.001634 0.001492 0.000857 0.001209 0.001842 0.001419 0.000790 0.001153 0.001731 0.001457
Nuclear Periphery Foci 0.001842 0.003697 0.001581 0.005326 0.001517 0.004317 0.004552 0.004220 0.001729 0.003991 0.002966 0.004801
Nucleolus 0.001585 0.001252 0.000843 0.017064 0.003816 0.002426 0.003949 0.011382 0.002362 0.001808 0.002291 0.014368
Nucleus 0.217335* 0.242543* 0.555687* 0.039579 0.209934* 0.153204* 0.354961* 0.073105 0.214759* 0.200205* 0.462111* 0.055486
Peroxisomes 0.001683 0.001406 0.000922 0.031105 0.004961 0.004459 0.013295 0.021469 0.002824 0.002853 0.006690 0.026533
Vacuole 0.027583 0.040771 0.014936 0.008727 0.037968 0.070234 0.008234 0.007305 0.031197 0.054734 0.011811 0.008052
Vacuole Periphery 0.000758 0.001346 0.000223 0.002253 0.002138 0.002875 0.001344 0.002317 0.001239 0.002070 0.000746 0.002283

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -7.38 -8.25 -5.17 -3.21 -0.09 -0.47 -2.13 -0.47 0.01 2.36 -3.17 -5.85 -4.70 -2.88 1.64
Bud Neck -3.27 -9.02 -14.50 -11.84 -6.13 1.07 -8.24 -8.80 -10.46 -1.32 -1.95 -13.38 -17.66 -15.80 -5.55
Bud Site -7.08 -9.10 -22.62 -17.56 -14.54 -4.54 -12.84 -17.69 -13.65 -6.30 -8.77 -16.39 -29.18 -22.08 -14.73
Cell Periphery 0.16 -5.22 -13.98 -15.06 -11.40 5.90 -8.93 -11.20 -14.77 -0.29 2.86 -10.71 -18.30 -21.08 -7.27
Cytoplasm -1.67 36.31 75.56* 73.01* 39.76* -10.59 27.38 39.84 59.37* 17.14 -6.80 46.93 82.73* 93.36* 40.04
Cytoplasmic Foci 15.58 26.23 -16.67 -26.32 -31.72* 13.55 8.01 -5.42 -16.80 -12.19 18.46 17.98 -16.97 -30.41 -29.94
Eisosomes 11.20 14.01 -1.61 -5.69 -6.88 13.10 6.64 12.42 0.47 0.74 15.61 8.77 5.19 -4.82 -3.61
Endoplasmic Reticulum -3.41 0.58 4.54 7.32 4.64 1.83 8.15 8.60 10.61 4.67 -1.51 6.38 9.06 11.43 5.13
Endosome 0.44 11.24 5.21 3.84 -3.99 0.74 9.87 9.26 11.49 -1.02 -0.54 13.95 10.74 11.37 -3.66
Golgi -0.18 4.69 -2.35 -1.87 -3.46 0.24 2.26 0.34 0.10 -1.62 -0.36 3.32 -1.72 -1.21 -3.62
Lipid Particles 7.97 7.67 2.12 -5.73 -5.40 9.50 6.27 9.12 -1.00 2.30 11.69 7.74 8.42 -4.79 -0.50
Mitochondria 0.85 1.75 1.30 0.20 -1.29 5.03 3.85 4.80 -0.51 1.59 3.97 3.69 4.30 -0.06 1.01
Mitotic Spindle -5.40 -13.22 -41.49* -40.51* -34.15* -7.81 -29.27* -37.06* -32.90* -10.22 -9.31 -30.02 -55.60 -51.89* -29.77*
None 2.63 1.64 8.84 8.05 17.38 6.31 4.56 6.39 0.65 10.65 7.15 4.70 9.51 6.91 18.78
Nuclear Periphery -2.69 -15.81 -11.22 -6.63 1.79 -2.69 -12.03 -11.19 -6.77 1.14 -3.91 -19.48 -15.93 -9.47 2.05
Nuclear Periphery Foci -4.00 -3.73 -7.12 -2.70 -4.54 -4.02 -8.61 -7.49 -1.01 1.60 -5.55 -8.93 -10.00 -2.65 -2.19
Nucleolus 0.36 0.62 -9.94 -9.94 -10.04 2.16 -2.91 -6.14 -7.21 -4.72 1.36 -3.64 -11.96 -12.23 -10.73
Nucleus -3.92 -41.09 20.80 22.72 58.20 6.33 -9.70 9.56 4.72 18.65 2.02 -33.01 20.99 19.23 49.96
Peroxisomes 0.73 4.56 -12.67 -12.56 -13.38 0.88 -0.90 -6.85 -6.70 -4.62 0.15 -0.69 -14.87 -13.97 -12.98
Vacuole -6.34 -2.88 3.75 8.91 6.21 -9.67 6.46 5.50 15.30 -0.90 -12.18 2.13 6.18 17.11 4.51
Vacuole Periphery -1.16 7.39 -1.45 0.22 -3.53 -0.82 4.34 2.96 2.50 -0.86 -1.85 5.51 0.68 2.04 -2.88
ORF
Human Ortholog
Description Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified gene MRPL38
Localization
Cell Percentages cytoplasm (54%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

YKL169C

YKL169C


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ykl169c-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available