Standard name | |
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Human Ortholog | |
Description | Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 5 | 8 | 7 | – | 20 | 18 | 22 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 3 | – | 6 | 4 | 7 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 2 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 3 | 2 | 4 | 8 | 9 | 20 | 19 | 17 | – | 58 | 46 | 54 | 4 | 7 | 5 | 24 | 11 | 22 | 0 | 0 | 0 | 0 | 1 | 0 |
Cytoplasm | 194 | 214 | 322 | 378 | 361 | 460 | 472 | 412 | – | 444 | 364 | 417 | 296 | 396 | 428 | 393 | 293 | 301 | 190 | 204 | 301 | 221 | 311 | 366 |
Endoplasmic Reticulum | 1 | 1 | 1 | 2 | 6 | 20 | 64 | 70 | – | 86 | 20 | 42 | 5 | 10 | 9 | 13 | 8 | 10 | 0 | 1 | 2 | 7 | 13 | 9 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
Golgi | 0 | 0 | 1 | 0 | 0 | 0 | 2 | 0 | – | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 1 | 0 | 1 | 6 | 13 | 9 | – | 22 | 56 | 62 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 4 | 3 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 3 | – | 2 | 2 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 2 | 1 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 4 | 0 | – | 1 | 2 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 0 | 1 | 0 | 0 | 0 | 7 | 6 | 4 | – | 36 | 26 | 9 | 1 | 1 | 3 | 1 | 0 | 0 | 0 | 0 | 0 | 2 | 5 | 5 |
Unique Cell Count | 195 | 216 | 324 | 381 | 367 | 502 | 561 | 499 | 609 | 479 | 546 | 303 | 406 | 434 | 397 | 296 | 302 | 194 | 211 | 311 | 237 | 348 | 403 | |
Labelled Cell Count | 198 | 218 | 329 | 388 | 377 | 520 | 590 | 525 | 675 | 539 | 613 | 307 | 415 | 446 | 432 | 313 | 333 | 194 | 211 | 311 | 237 | 348 | 403 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 58.2 | 55.8 | 33.9 | 31.7 | 26.1 | 21.9 | 19.5 | 19.4 | – | 17.3 | 16.5 | 16.3 | 35.4 | 36.0 | 37.4 | 58.7 | 59.2 | 61.2 | 44.9 | 46.1 | 45.1 |
Std Deviation (1e-4) | 8.9 | 10.6 | 6.6 | 5.7 | 4.9 | 4.4 | 4.4 | 4.5 | – | 4.1 | 4.1 | 4.0 | 8.0 | 7.2 | 7.0 | 13.1 | 13.8 | 14.4 | 9.2 | 11.9 | 12.2 |
Intensity Change (Log2) | – | – | – | -0.1 | -0.37 | -0.63 | -0.8 | -0.81 | – | -0.97 | -1.03 | -1.06 | 0.06 | 0.09 | 0.14 | 0.79 | 0.81 | 0.85 | 0.41 | 0.44 | 0.41 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | – | 3.3 | 3.5 | 3.7 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0.9 | 1.2 | 2.3 | 1.9 | 1.9 | – | 4.8 | 4.8 | 4.9 | 0 | 0 | 0 | 3.3 | 2.0 | 3.8 |
Cytoplasm | -0.3 | -1.2 | -4.8 | -7.2 | -7.6 | – | -10.0 | -9.2 | -9.2 | -1.8 | -1.9 | -1.0 | -0.6 | -0.6 | 0.5 |
Endoplasmic Reticulum | 0 | 0 | 3.3 | 6.1 | 6.8 | – | 6.9 | 3.4 | 4.9 | 0 | 0 | 0 | 2.9 | 0 | 2.9 |
Endosome | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 2.3 | 0 | – | 3.1 | 6.2 | 6.1 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 0 | 0 | – | 4.5 | 4.3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 41.2731 | 51.6152 | 45.1906 | 43.0472 | 42.3359 | 45.6532 | 42.9803 | 53.1603 | 46.187 | 42.2843 | 46.8844 | 47.5873 | 34.9273 | 47.0027 | 41.1432 | 40.9954 | 27.9443 | 42.808 |
Actin | 0.0002 | 0.0001 | 0.0001 | 0 | 0.0001 | 0.0001 | 0.0004 | 0.0002 | 0.0003 | 0.0001 | 0.0002 | 0.0002 | 0.002 | 0.0002 | 0.0003 | 0 | 0.0096 | 0.0047 |
Bud | 0.0004 | 0.0001 | 0 | 0.0001 | 0.0001 | 0 | 0.0003 | 0.0001 | 0.0002 | 0.0002 | 0.0003 | 0.0001 | 0.0007 | 0.0001 | 0.0003 | 0.0001 | 0.0045 | 0.0001 |
Bud Neck | 0.0001 | 0.0001 | 0.0002 | 0.0002 | 0.0004 | 0.0005 | 0.0002 | 0.0001 | 0.0002 | 0.0002 | 0.0002 | 0.0005 | 0.0002 | 0.0001 | 0.0002 | 0.0001 | 0.0011 | 0.0003 |
Bud Periphery | 0.0003 | 0 | 0 | 0 | 0 | 0 | 0.0002 | 0 | 0.0001 | 0 | 0.0002 | 0 | 0.0004 | 0 | 0.0003 | 0 | 0.0043 | 0.0001 |
Bud Site | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0.0002 | 0.0001 | 0.0001 | 0 | 0.0001 | 0 | 0.0012 | 0.0001 | 0.0001 | 0 | 0.0028 | 0.0001 |
Cell Periphery | 0.0002 | 0.0001 | 0 | 0.0003 | 0 | 0 | 0.0001 | 0.0001 | 0.0001 | 0 | 0.0001 | 0 | 0.0002 | 0 | 0.0001 | 0.0003 | 0.0005 | 0 |
Cytoplasm | 0.9679 | 0.9949 | 0.9898 | 0.9783 | 0.9895 | 0.9873 | 0.9656 | 0.9933 | 0.9823 | 0.9919 | 0.9767 | 0.9903 | 0.8934 | 0.9841 | 0.9739 | 0.9912 | 0.8254 | 0.9122 |
Cytoplasmic Foci | 0.0008 | 0.0004 | 0.0006 | 0.0007 | 0.0012 | 0.0007 | 0.0024 | 0.0005 | 0.0016 | 0.001 | 0.002 | 0.0023 | 0.0214 | 0.0019 | 0.0009 | 0.0005 | 0.0249 | 0.0042 |
Eisosomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0.0001 | 0 | 0 | 0 | 0.0001 | 0 |
Endoplasmic Reticulum | 0.0067 | 0.0023 | 0.0052 | 0.002 | 0.0035 | 0.0072 | 0.012 | 0.0037 | 0.0063 | 0.0006 | 0.0069 | 0.0028 | 0.0106 | 0.0004 | 0.0054 | 0.0008 | 0.002 | 0.0022 |
Endosome | 0.0012 | 0.0003 | 0.0006 | 0.0012 | 0.0014 | 0.0008 | 0.0045 | 0.0004 | 0.0012 | 0.0012 | 0.0043 | 0.0017 | 0.0179 | 0.0019 | 0.0013 | 0.0008 | 0.0281 | 0.0124 |
Golgi | 0.0001 | 0 | 0 | 0 | 0.0001 | 0 | 0.0003 | 0.0001 | 0.0002 | 0 | 0.0002 | 0.0001 | 0.0046 | 0.0003 | 0.0001 | 0 | 0.0187 | 0.0098 |
Lipid Particles | 0.0001 | 0 | 0 | 0.0001 | 0 | 0 | 0.0002 | 0 | 0.0003 | 0 | 0 | 0 | 0.0059 | 0.0003 | 0.0001 | 0.0002 | 0.0024 | 0.0056 |
Mitochondria | 0.0011 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0.0004 | 0.0001 | 0.0029 | 0 | 0.0008 | 0.0001 | 0.0028 | 0.0014 | 0.0002 | 0 | 0.0492 | 0.0052 |
None | 0.0007 | 0.0001 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0.0019 | 0.0001 | 0.001 | 0.0002 | 0.0007 | 0.0002 | 0.0047 | 0.0004 | 0.0043 | 0.0001 | 0.0013 | 0.0002 |
Nuclear Periphery | 0.0048 | 0.0003 | 0.0007 | 0.0011 | 0.0004 | 0.001 | 0.0029 | 0.0002 | 0.0009 | 0.0009 | 0.0005 | 0.0004 | 0.0076 | 0.0009 | 0.0015 | 0.001 | 0.0008 | 0.0358 |
Nucleolus | 0.0007 | 0 | 0 | 0 | 0 | 0 | 0.0002 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0.001 | 0.0002 | 0.0002 | 0 | 0.0003 | 0 |
Nucleus | 0.0058 | 0.0005 | 0.0009 | 0.0013 | 0.0009 | 0.001 | 0.0021 | 0.0004 | 0.0007 | 0.001 | 0.0011 | 0.0006 | 0.0047 | 0.0058 | 0.0023 | 0.0007 | 0.0015 | 0.0018 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0.0001 | 0 | 0 | 0 | 0.0024 | 0.0001 | 0 | 0 | 0.0016 | 0.0004 |
Punctate Nuclear | 0.001 | 0 | 0 | 0 | 0 | 0 | 0.0002 | 0 | 0 | 0.0001 | 0 | 0 | 0.002 | 0.0001 | 0.0006 | 0 | 0.0005 | 0.0002 |
Vacuole | 0.0059 | 0.0006 | 0.0014 | 0.0133 | 0.002 | 0.001 | 0.0044 | 0.0005 | 0.0011 | 0.0019 | 0.0045 | 0.0006 | 0.0132 | 0.001 | 0.0067 | 0.0034 | 0.0178 | 0.0007 |
Vacuole Periphery | 0.0019 | 0.0001 | 0.0001 | 0.0009 | 0.0003 | 0.0002 | 0.0013 | 0 | 0.0004 | 0.0004 | 0.0011 | 0.0002 | 0.0029 | 0.0006 | 0.0014 | 0.0005 | 0.0028 | 0.004 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 61.8058 | 69.8259 | 80.4876 | 79.6456 | 78.6591 | 78.8251 | 81.7394 | 93.9139 | 82.8307 | 69.2661 |
Translational Efficiency | 1.5013 | 1.3539 | 1.0908 | 1.2255 | 1.0729 | 1.2233 | 1.3366 | 1.1819 | 1.1081 | 1.0992 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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