Standard name
Human Ortholog
Description Poly(A) polymerase; one of three factors required for mRNA 3'-end polyadenylation, forms multiprotein complex with polyadenylation factor I (PF I), also required for mRNA nuclear export; may also polyadenylate rRNAs; required for gene looping
DOWNLOAD

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0.05 0 – 0 0.05 0 0 0.07 0.05 0.16 0.05 0.08 0.05 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 – 0.06 0 0.13 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0.94 0.91 0.98 0.92 0.9 0.83 0.88 – 0.81 0.78 0.75 0.76 0.88 0.89 0.78 0.88 0.87 0.86 0.9 0.87 0.92 0.84 0.84 0.81
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.21 0.07 0 0.08 0.05 0.17 0.13 – 0.3 0.28 0.44 0.36 0.08 0.1 0.06 0.08 0.08 0.13 0 0 0 0.05 0.08 0.07
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 1 – 1 2 2 1 0 1 0 0 0 0 1 2 0 0 0 0
Bud Neck 0 0 0 1 0 0 0 – 0 2 0 2 0 1 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 2 – 4 6 7 13 1 1 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 2 6 2 6 9 12 15 – 5 14 3 6 9 8 29 11 15 8 0 0 0 0 1 1
Endoplasmic Reticulum 0 2 0 0 0 0 0 – 0 0 0 0 0 0 0 3 2 2 0 1 0 0 0 0
Endosome 0 0 0 2 1 0 2 – 0 0 0 0 0 1 1 0 1 0 1 1 0 0 0 1
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 3 1 0 1 0
Mitochondria 4 1 0 0 0 5 6 – 16 9 43 15 1 0 0 1 0 5 0 0 0 0 0 1
Nucleus 123 195 189 145 197 200 328 – 222 242 250 288 108 150 144 201 170 144 130 199 184 49 99 133
Nuclear Periphery 0 1 0 0 2 4 5 – 1 1 3 4 0 0 2 1 1 0 0 0 0 0 0 0
Nucleolus 27 14 6 13 12 41 49 – 83 88 146 137 10 16 12 18 15 22 5 9 8 3 9 11
Peroxisomes 0 0 0 0 1 2 0 – 0 0 0 1 0 0 1 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 2 1 – 0 0 0 3 3 7 6 1 3 0 0 1 0 0 1 0
Vac/Vac Membrane 0 9 0 3 2 2 1 – 1 1 3 3 1 1 1 5 5 3 2 7 2 1 2 9
Unique Cell Count 131 215 192 157 219 241 373 273 310 335 380 123 168 185 228 196 168 145 228 201 59 119 164
Labelled Cell Count 156 228 197 170 224 268 410 333 365 457 473 133 186 196 241 212 184 145 228 201 59 119 164


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 7.6 11.4 9.8 9.0 9.9 8.7 8.3 – 7.8 8.2 7.4 7.6 9.3 11.2 10.2 13.3 13.7 13.2 7.5 9.3 10.1
Std Deviation (1e-4) 1.6 3.2 1.6 1.8 2.6 2.3 1.7 – 1.9 2.2 2.0 2.2 2.1 10.3 2.7 4.2 5.2 4.5 1.4 1.7 2.0
Intensity Change (Log2) – – – -0.12 0.01 -0.17 -0.24 – -0.32 -0.26 -0.4 -0.36 -0.07 0.19 0.06 0.44 0.48 0.43 -0.39 -0.07 0.05


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 1.9 2.3 2.0 – 0 2.2 0 0 0 0 5.2 2.2 3.2 0
Endoplasmic Reticulum 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 – 3.4 0 5.2 2.8 0 0 0 0 0 0
Nucleus -2.8 -3.6 -5.3 -4.2 – -5.7 -6.4 -7.1 -6.9 -4.0 -3.7 -6.2 -4.1 -4.4 -4.6
Nuclear Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nucleolus 2.1 1.2 4.6 3.8 – 7.4 7.1 9.9 8.6 2.0 2.5 1.5 2.1 2.0 3.5
Peroxisomes 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 5.519 6.0547 5.3793 5.4645 4.1959 5.3472 5.3878 5.795 5.4026 4.99 4.6804 5.2775 5.3224 5.7931 5.7937 4.9276 4.2163 4.9799
Actin 0.0113 0 0.007 0 0.0376 0 0.012 0 0.0009 0.0044 0.0052 0.0001 0.009 0.0026 0.006 0.0049 0.0936 0.0038
Bud 0.0003 0 0.0055 0 0.0004 0.0001 0.0006 0 0.0001 0.0004 0.0008 0.0001 0.0007 0.0001 0.0001 0.0002 0.0005 0.0001
Bud Neck 0.0005 0 0.0002 0 0.0023 0.0004 0.0011 0 0.0001 0.0001 0.0003 0.0007 0.0101 0.0015 0.0001 0.0003 0.0002 0.0005
Bud Periphery 0.0005 0 0.0023 0 0.0012 0.0002 0.0015 0 0.0001 0.0006 0.0012 0.0002 0.0014 0.0001 0.0002 0.0002 0.0011 0.0001
Bud Site 0.0004 0 0.0039 0 0.0007 0.0001 0.0017 0 0.0004 0.0005 0.0009 0.0002 0.0082 0.0021 0.0004 0.0013 0.0007 0.0004
Cell Periphery 0.0001 0 0.0002 0 0.0003 0.0001 0.0009 0 0 0 0.0001 0.0001 0.0004 0 0 0.0001 0.0002 0.0001
Cytoplasm 0.0018 0 0.0011 0 0.0027 0 0.002 0 0.0004 0.0008 0.0042 0.0003 0.0222 0.0002 0.0007 0.0016 0.0001 0.0008
Cytoplasmic Foci 0.0079 0 0.001 0 0.0078 0 0.0101 0 0.0037 0.0007 0.0265 0.0002 0.0368 0.0066 0.0127 0.002 0.0034 0.0077
Eisosomes 0.0001 0 0 0 0.0005 0 0.0002 0 0 0 0 0 0.0001 0 0.0001 0.0001 0.0002 0
Endoplasmic Reticulum 0.0017 0 0.0007 0 0.0031 0 0.0014 0 0.0007 0.0009 0.0046 0.0001 0.0023 0 0.0003 0.0018 0.0001 0.0003
Endosome 0.007 0 0.0022 0 0.0216 0 0.0086 0 0.0032 0.0052 0.0704 0.0002 0.026 0.0022 0.0053 0.0083 0.0016 0.0048
Golgi 0.0014 0 0.0009 0 0.0115 0 0.0043 0 0.0016 0.0008 0.018 0.0001 0.0116 0.0015 0.0051 0.0011 0.006 0.0029
Lipid Particles 0.0092 0 0.0008 0 0.0243 0 0.0097 0 0.0019 0.0008 0.0113 0.0001 0.032 0.0028 0.0098 0.0015 0.0037 0.0171
Mitochondria 0.0014 0.0001 0.0031 0 0.0077 0.0015 0.0072 0.0001 0.0011 0.0052 0.0123 0.0002 0.0053 0.0004 0.0025 0.001 0.0144 0.0022
None 0.0077 0 0.0007 0 0.0015 0 0.0024 0 0.0003 0.0008 0.0012 0 0.0095 0.0002 0.0025 0.0022 0.0003 0.0006
Nuclear Periphery 0.0054 0.0002 0.0011 0.0001 0.0259 0.0009 0.0109 0.0002 0.0046 0.0246 0.0199 0.0006 0.0106 0.0001 0.0009 0.0058 0.0006 0.0017
Nucleolus 0.0446 0.0246 0.0236 0.0135 0.132 0.1209 0.0287 0.0154 0.0148 0.0262 0.0922 0.0851 0.0257 0.015 0.0111 0.0146 0.148 0.0875
Nucleus 0.8757 0.9748 0.9437 0.9862 0.6943 0.8749 0.8799 0.9842 0.9573 0.9209 0.7096 0.9033 0.7518 0.9581 0.9346 0.9455 0.7111 0.8569
Peroxisomes 0.0085 0 0.0004 0 0.0168 0 0.002 0 0.0021 0.0006 0.0045 0 0.0172 0.0053 0.0048 0.0004 0.0118 0.008
Punctate Nuclear 0.013 0.0002 0.0006 0.0001 0.0048 0.0003 0.0097 0.0001 0.0055 0.0037 0.0085 0.0083 0.0148 0.0009 0.0025 0.0058 0.0017 0.003
Vacuole 0.0009 0 0.0005 0 0.0011 0.0002 0.0039 0 0.0007 0.0009 0.0044 0.0002 0.0028 0.0002 0.0002 0.0007 0.0002 0.0007
Vacuole Periphery 0.0006 0 0.0004 0 0.0018 0.0003 0.0013 0 0.0005 0.0018 0.0037 0 0.0012 0.0001 0.0002 0.0004 0.0005 0.0009

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 22.3008 20.8023 24.0119 29.0657 30.5901 13.5669 23.0781 27.0118 29.4349 28.7298
Translational Efficiency 1.1054 1.2321 1.0117 1.102 0.7985 1.9554 1.0639 0.9155 0.9262 0.7952

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Poly(A) polymerase; one of three factors required for mRNA 3'-end polyadenylation, forms multiprotein complex with polyadenylation factor I (PF I), also required for mRNA nuclear export; may also polyadenylate rRNAs; required for gene looping
Localization
Cell Percentages nucleus (100%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-4

Pap1

Pap1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Pap1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available