Standard name
Human Ortholog
Description Subunit of the EGO/GSE complex; the vacuolar/endosomal membrane associated EGO/GSE complex regulates exit from rapamycin-induced growth arrest, stimulating microautophagy and sorting of Gap1p from the endosome to the plasma membrane; loss results in a defect in vacuolar acidification
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0.05 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.07 0 0.05 0.09 0.1 0.17 0.13 – 0.2 0.23 0.28 0.27 0 0 0 0 0 0 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0.05 0 – 0.07 0.13 0.08 0.06 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 – 0 0 0 0 0.07 0 0 0 0 0 0 0 0 0.06 0.06 0.07
Golgi 0 0 0 0.05 0.16 0 0.12 – 0.05 0.06 0.06 0.05 0 0 0 0.08 0.1 0.09 0 0 0 0 0 0
Mitochondria 0 0.07 0 0.1 0 0.22 0.05 – 0.2 0.14 0.21 0.23 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0.05 0.05 0 0 0 0.07 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0
Nuclear Periphery 0.09 0.07 0.07 0.09 0 0.09 0.13 – 0.09 0.08 0.06 0.05 0.11 0.14 0.1 0.24 0.17 0.14 0.07 0 0.09 0 0.08 0.06
Nucleolus 0.06 0.07 0.05 0 0 0 0 – 0 0 0 0 0.09 0.05 0.07 0 0 0.05 0 0 0 0 0 0.05
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.79 0.83 0.84 0.86 0.8 0.6 0.68 – 0.55 0.48 0.45 0.45 0.85 0.88 0.87 0.89 0.91 0.88 0.81 0.86 0.81 0.8 0.78 0.73
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 3 – 4 3 1 0 0 0 0 0 0 1 0 0 0 0 0 0
Bud 0 0 0 0 0 0 1 – 1 0 0 0 0 0 0 0 0 0 2 1 2 0 1 5
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 3 2 2 13 – 17 19 4 7 0 1 0 3 5 1 0 0 0 0 0 0
Cytoplasm 27 11 17 26 37 57 75 – 66 85 50 55 6 14 15 9 8 9 0 0 0 0 0 0
Endoplasmic Reticulum 3 2 0 1 1 16 21 – 23 47 14 12 1 2 2 5 7 7 0 0 0 0 1 1
Endosome 6 2 13 5 6 2 9 – 0 4 1 2 25 25 23 8 7 5 13 13 15 19 32 37
Golgi 4 2 7 15 56 13 69 – 16 22 10 11 2 6 3 22 31 21 1 0 0 2 0 0
Mitochondria 16 19 7 29 8 75 28 – 67 50 37 47 5 2 3 4 6 6 8 4 5 5 12 9
Nucleus 20 13 14 1 15 23 7 – 10 7 6 7 14 19 19 12 10 8 9 5 3 14 12 20
Nuclear Periphery 33 18 22 26 12 30 73 – 31 28 10 11 44 84 59 67 50 32 27 9 30 10 46 31
Nucleolus 22 20 17 8 0 5 0 – 2 1 1 0 35 29 41 12 12 11 9 2 4 7 12 23
Peroxisomes 0 1 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0
SpindlePole 0 2 1 1 0 0 0 – 0 0 0 0 1 2 1 2 0 2 2 2 0 0 1 2
Vac/Vac Membrane 287 226 275 260 284 201 390 – 185 172 80 91 325 512 527 249 273 196 332 250 284 245 432 365
Unique Cell Count 364 272 326 304 356 336 570 338 362 177 203 383 584 605 280 300 222 410 292 352 307 557 501
Labelled Cell Count 418 316 373 375 421 424 689 422 438 214 243 458 696 693 393 409 300 410 292 352 307 557 501


Vacuole Periphery

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 14.3 14.3 12.7 12.3 12.8 8.7 11.3 – 10.3 10.5 9.8 10.0 15.9 16.7 16.7 17.6 18.0 16.1 13.5 14.2 15.3
Std Deviation (1e-4) 4.3 3.7 2.7 2.5 2.7 2.4 2.3 – 2.2 2.1 2.2 2.3 3.5 3.7 3.7 5.1 4.4 4.5 3.6 3.8 3.8
Intensity Change (Log2) – – – -0.05 0.01 -0.55 -0.17 – -0.3 -0.29 -0.38 -0.36 0.32 0.39 0.39 0.46 0.5 0.34 0.08 0.15 0.26

WT3RAP60RAP140RAP220RAP300RAP460RAP540RAP620RAP70005101520WT3HU80HU120HU16005101520WT3rpd3Δ_1rpd3Δ_2rpd3Δ_305101520WT1AF100AF140AF18005101520
rapamycinhydroxy-urearpd3 knockoutalpha-factorMultiple Condition Time-Course Intensity PlotsGFP Intensity (1e-4)GFP Intensity (1e-4)GFP Intensity (1e-4)GFP Intensity (1e-4)

Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 – 4.1 4.2 0 0 0 0 0 0 0 0
Cytoplasm 1.7 2.5 4.8 3.8 – 5.6 6.7 7.3 7.1 -2.7 -2.2 -2.2 -1.2 -1.6 -0.6
Endoplasmic Reticulum 0 0 4.0 3.5 – 4.8 6.7 0 0 0 0 0 0 0 0
Endosome -1.8 -1.8 -2.9 -2.2 – -3.7 -2.4 0 -2.0 1.5 0.2 -0.1 -0.8 -1.2 -1.1
Golgi 1.9 6.1 1.3 5.1 – 1.8 2.6 2.1 2.0 0 0 0 3.3 4.3 3.8
Mitochondria 4.0 0.1 7.9 2.1 – 7.2 5.5 7.1 7.8 -0.9 0 0 -0.7 -0.1 0.4
Nucleus -3.3 -0.1 1.4 -2.9 – -0.9 -1.8 -0.5 -0.5 -0.4 -0.8 -0.9 0 -0.6 -0.4
Nuclear Periphery 0.9 -2.0 1.0 2.8 – 1.2 0.5 -0.5 -0.6 2.2 3.4 1.6 6.0 3.9 3.0
Nucleolus -1.7 -4.4 -2.7 -5.5 – -3.6 -4.1 -2.7 -3.3 2.0 -0.2 0.9 -0.5 -0.7 -0.1
Peroxisomes 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Vacuole 0.4 -1.6 -7.0 -5.2 – -8.3 -10.1 -9.2 -9.6 0.2 1.4 1.2 1.6 2.5 1.3

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 6.2472 7.5658 7.0461 6.5452 6.9037 6.8825 8.5973 9.5614 8.9335 8.613 8.0983 8.8021 10.7946 12.4098 10.8584 10.0785 10.3589 10.9454
Actin 0.0078 0 0.0047 0.0001 0 0 0 0 0 0.0001 0 0 0.0011 0 0.0048 0.0353 0 0.0038
Bud 0.0001 0.0001 0.0014 0.0002 0 0 0 0.0001 0.0002 0.0001 0 0.0001 0.0003 0.0001 0.0002 0.0002 0.0001 0.0001
Bud Neck 0.0002 0.0001 0.0001 0.0032 0 0 0 0 0.0001 0 0 0 0.0002 0 0.0002 0.0003 0 0.0001
Bud Periphery 0.0003 0.0003 0.0024 0.0009 0.0003 0.0002 0.0001 0.0001 0.0004 0.0001 0.0002 0.0002 0.0011 0.0005 0.0006 0.0004 0.0002 0.0002
Bud Site 0.0002 0.0001 0.0002 0.0005 0 0 0 0.0001 0.0003 0 0 0.0001 0.0002 0 0.0003 0.0004 0 0
Cell Periphery 0.0005 0.0008 0.0007 0.0008 0.0004 0.0002 0.0005 0.0004 0.0005 0.0004 0.0001 0.0002 0.0007 0.0004 0.0006 0.0002 0.0002 0.0002
Cytoplasm 0.001 0.0008 0.0004 0.0002 0.0001 0.0001 0.0002 0.0004 0.0003 0.0002 0.0002 0.0002 0.0005 0.0014 0.0008 0.0002 0.0001 0.0003
Cytoplasmic Foci 0.0056 0.0013 0.0014 0.0002 0.0001 0.0002 0.0033 0.0028 0.0043 0.0002 0.0009 0.0001 0.001 0.0005 0.0028 0.0049 0.0002 0.0006
Eisosomes 0.0001 0 0 0 0 0 0 0 0 0 0 0 0.0001 0 0 0.0002 0 0
Endoplasmic Reticulum 0.001 0.0005 0.0011 0.0005 0.0022 0.0003 0.0006 0.001 0.0003 0.0017 0.0003 0.0002 0.0011 0.0008 0.0009 0.0004 0.0002 0.0004
Endosome 0.0305 0.0174 0.0229 0.0172 0.0111 0.0117 0.0205 0.014 0.0293 0.013 0.0362 0.0126 0.0341 0.0192 0.0228 0.0076 0.0213 0.0147
Golgi 0.0024 0.0005 0.0032 0.0013 0.0001 0.0003 0.001 0.0003 0.0019 0.0006 0.0021 0.0003 0.0044 0.0004 0.0062 0.0055 0.0005 0.0006
Lipid Particles 0.0043 0.0026 0.0074 0.0014 0.0003 0.002 0.0096 0.0021 0.0057 0.0028 0.0025 0.0013 0.0026 0.0025 0.0029 0.0088 0.0018 0.0041
Mitochondria 0.0112 0.0039 0.0184 0.0069 0.0026 0.0014 0.0048 0.0015 0.0069 0.0006 0.0012 0.0016 0.0197 0.001 0.0093 0.0574 0.0022 0.0037
None 0.0016 0 0.0001 0.0001 0 0 0.0001 0 0 0 0 0 0.0004 0 0.0011 0.0003 0 0.0003
Nuclear Periphery 0.0056 0.0095 0.0084 0.0033 0.0059 0.0192 0.0094 0.0167 0.0077 0.0226 0.0044 0.0103 0.0086 0.0127 0.0182 0.0075 0.0043 0.0123
Nucleolus 0.001 0.0091 0.0058 0.0021 0.0001 0.0006 0.0005 0.0012 0.0011 0.0002 0.0001 0.0004 0.001 0.0004 0.0007 0.0002 0.0002 0.0031
Nucleus 0.0035 0.0019 0.006 0.0024 0.0007 0.0006 0.001 0.0012 0.0006 0.0006 0.0003 0.0005 0.0014 0.0006 0.0019 0.0004 0.0004 0.0011
Peroxisomes 0.001 0.0001 0.0006 0.0001 0 0 0.0002 0 0.0005 0 0.0001 0 0.0003 0 0.0012 0.0074 0 0.0001
Punctate Nuclear 0.0012 0 0.0001 0 0 0 0.0002 0 0 0.0001 0 0 0.0002 0 0.0005 0.0014 0 0.0003
Vacuole 0.3036 0.2656 0.2938 0.2525 0.2525 0.2136 0.2694 0.2375 0.2865 0.2986 0.2195 0.201 0.2701 0.2646 0.2344 0.2101 0.2133 0.1716
Vacuole Periphery 0.6171 0.6854 0.6207 0.7062 0.7234 0.7496 0.6784 0.7204 0.6534 0.6581 0.7317 0.7709 0.6509 0.6948 0.6895 0.6512 0.7547 0.7824

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 24.8515 10.6635 23.7384 28.8463 37.3764 17.8831 25.607 30.9274 34.187 31.7095
Translational Efficiency 1.1538 2.2772 1.0607 0.8811 0.7427 1.8233 1.062 1.2688 1.1014 1.2648

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1770 1645 3077 123 1130 2479 1666 1645 2900 4124 4743 1768

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 1156.76 1164.25 1529.68 1560.12 1111.58 1247.84 1478.71 1464.95 1139.16 1214.50 1511.78 1471.57
Standard Deviation 172.83 226.84 256.46 249.19 201.20 222.75 230.14 245.44 185.71 228.09 248.73 246.89
Intensity Change Log 2 — 0.009311 0.403140 0.431567 — 0.166821 0.411727 0.398240 — 0.088647 0.407355 0.415332

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000013 0.000139 0.000024 0.000381 0.000063 0.000097 0.000053 0.000027 0.000032 0.000114 0.000034 0.000052
Bud Neck 0.000154 0.000899 0.000627 0.006048 0.000549 0.002731 0.001138 0.000752 0.000308 0.002000 0.000806 0.001121
Bud Site 0.001113 0.005339 0.000279 0.007668 0.002723 0.003066 0.000714 0.001397 0.001740 0.003973 0.000432 0.001833
Cell Periphery 0.000867 0.001065 0.000492 0.000281 0.000401 0.000746 0.000298 0.000992 0.000685 0.000873 0.000424 0.000943
Cytoplasm 0.000296 0.002909 0.000041 0.000244 0.001981 0.000824 0.000048 0.000167 0.000953 0.001655 0.000043 0.000172
Cytoplasmic Foci 0.000327 0.008064 0.000703 0.003475 0.002187 0.004470 0.001129 0.001132 0.001052 0.005904 0.000853 0.001295
Eisosomes 0.000003 0.000016 0.000091 0.000038 0.000006 0.000054 0.000046 0.000025 0.000004 0.000039 0.000075 0.000026
Endoplasmic Reticulum 0.000581 0.000740 0.000229 0.000247 0.000625 0.000858 0.000234 0.000325 0.000598 0.000811 0.000231 0.000319
Endosome 0.001872 0.018819 0.000386 0.004760 0.006149 0.010503 0.001092 0.002217 0.003539 0.013820 0.000634 0.002394
Golgi 0.000915 0.014898 0.000231 0.021991 0.002793 0.002989 0.001155 0.004915 0.001647 0.007739 0.000556 0.006103
Lipid Particles 0.000171 0.002104 0.001205 0.000955 0.000314 0.003461 0.000911 0.000739 0.000226 0.002920 0.001102 0.000754
Mitochondria 0.003724 0.015394 0.003581 0.012307 0.006928 0.023952 0.006185 0.007614 0.004972 0.020538 0.004496 0.007941
Mitotic Spindle 0.000062 0.000531 0.000068 0.001125 0.000581 0.000977 0.000259 0.000626 0.000264 0.000800 0.000135 0.000660
None 0.000082 0.000919 0.000296 0.000269 0.000191 0.000915 0.000296 0.000264 0.000124 0.000917 0.000296 0.000264
Nuclear Periphery 0.000397 0.000703 0.002236 0.005261 0.000684 0.004188 0.001894 0.000791 0.000509 0.002798 0.002116 0.001102
Nuclear Periphery Foci 0.000197 0.001676 0.000894 0.001822 0.000532 0.005066 0.001132 0.000571 0.000327 0.003714 0.000978 0.000658
Nucleolus 0.000345 0.002265 0.006647 0.003545 0.001231 0.009485 0.012242 0.002564 0.000690 0.006605 0.008612 0.002632
Nucleus 0.000297 0.000892 0.003365 0.001555 0.001454 0.003930 0.003038 0.000972 0.000748 0.002718 0.003250 0.001013
Peroxisomes 0.000060 0.001352 0.000149 0.002084 0.000426 0.000977 0.000258 0.000112 0.000202 0.001126 0.000187 0.000249
Vacuole 0.146144 0.236314 0.247835 0.341561 0.168483 0.268130 0.347994 0.363171 0.154848 0.255439 0.283016 0.361667
Vacuole Periphery 0.842382 0.684963 0.730622 0.584384 0.801700 0.652581 0.619886 0.610626 0.826530 0.665497 0.691726 0.608801

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -3.96 -2.82 -1.42 -0.94 -1.38 -2.02 0.31 2.40 6.30 2.74 -5.39 -0.47 -1.02 2.62 -0.88
Bud Neck -7.60 -6.00 -2.27 -1.99 -2.09 -7.50 -3.53 -1.29 7.25 2.91 -9.90 -6.12 -3.90 3.43 -1.46
Bud Site -5.88 2.42 -1.85 -0.65 -2.09 -0.54 3.23 1.87 3.25 -1.97 -4.84 4.08 -0.27 4.04 -3.57
Cell Periphery -0.63 1.25 1.96 6.22 2.78 -3.20 1.86 -3.90 -1.42 -4.72 -0.96 1.42 -1.14 -0.45 -3.73
Cytoplasm -3.39 1.37 0.24 3.54 -2.21 1.52 2.71 2.53 2.43 -1.88 -1.55 3.02 2.54 4.32 -2.20
Cytoplasmic Foci -7.61 -1.70 -1.77 2.25 -1.54 -3.14 2.00 1.72 4.94 -0.01 -8.63 0.77 -0.60 7.16 -1.09
Eisosomes -5.92 -7.99 -2.61 -1.60 3.04 -8.78 -8.78 -5.86 4.78 3.96 -10.28 -9.67 -7.25 2.97 6.26
Endoplasmic Reticulum -1.09 2.85 2.24 4.17 -0.21 -2.11 3.87 3.03 9.38 -2.69 -2.24 4.31 3.22 9.74 -3.55
Endosome -10.42 4.57 -2.60 7.29 -4.08 -3.64 5.07 3.94 11.55 -4.35 -11.75 6.65 2.42 14.54 -8.70
Golgi -11.24 4.65 -2.40 -0.79 -2.47 -0.40 3.24 -2.16 -2.15 -4.29 -10.91 5.07 -4.32 1.46 -5.48
Lipid Particles -4.37 -17.64 -1.96 1.90 0.58 -9.39 -4.91 -4.37 8.35 1.82 -10.15 -14.86 -8.62 8.11 5.15
Mitochondria -6.55 0.15 -2.19 0.73 -2.24 -7.80 0.48 -0.42 7.82 -1.02 -10.96 0.59 -2.43 7.74 -3.06
Mitotic Spindle -5.87 -0.18 -1.91 -1.05 -1.90 -0.85 0.59 -0.07 0.62 -0.53 -2.35 0.63 -0.77 0.29 -1.01
None -4.84 -15.78 -3.86 3.62 0.55 -7.33 -3.82 -2.80 6.70 1.51 -8.75 -12.64 -8.71 7.17 1.93
Nuclear Periphery -2.49 -5.45 -2.82 -2.64 -1.72 -7.79 -3.37 -0.52 8.15 3.48 -8.70 -6.32 -3.55 5.85 3.60
Nuclear Periphery Foci -4.27 -6.20 -3.65 -0.11 -2.04 -8.39 -3.04 -0.29 8.44 3.20 -9.66 -6.36 -3.90 8.66 2.86
Nucleolus -3.96 -12.24 -3.39 -1.21 2.81 -7.85 -8.20 -2.14 6.96 7.45 -9.49 -13.53 -4.83 5.81 9.16
Nucleus -3.21 -7.58 -2.26 -1.17 2.57 -3.30 -2.46 0.81 5.93 6.20 -5.25 -6.93 -1.00 5.23 7.24
Peroxisomes -3.87 -3.01 -1.13 -0.40 -1.08 -2.88 1.01 1.99 7.32 3.85 -5.72 0.18 -0.34 4.50 -0.47
Vacuole -15.35 -18.95 -9.56 -5.12 -4.59 -15.62 -24.63 -26.02 -13.84 -1.98 -23.65 -29.23 -33.54 -17.21 -12.57
Vacuole Periphery 20.53 19.09 10.70 4.09 6.08 18.19 21.48 22.18 5.18 1.10 29.68 27.37 32.13 7.92 12.32
Protein Complexes26S Proteasome complex37S mitochondrial small ribosomal subunit54S mitochondrial large ribosomal subunit60S cytosolic large ribosomal subunitAar2-U5 snRNPAnaphase-Promoting Complex variant 1Anaphase-Promoting Complex variant 2Anaphase-Promoting Complex variant 3Anaphase-Promoting Complex variant 4Arp2/3 complexATG1 kinase complexBLOC-1 complexCcr4-Not complexCentral kinetochore CTF19 complexCleavage and polyadenylation specificity factorcomplexCMG helicase complexCMG-Pol epsilon complexCOMPASS complexCOP9 signalosome COPI vesicle coat complexCoQ6 biosynthesis complexCore mediator complexCORVET complexCTF18-RFC complexCURI complex variant 1CURI complex variant 2CURI complex variant 3Cytoplasmic exosome complex, DIS3 variantDASH complexDNA-directed RNA Polymerase I complexDNA-directed RNA polymerase II complexDNA-directed RNA polymerase III complexDNA replication factor C complexEGO complexELG1-RFC complex Elongator holoenzyme complexEndoplasmic Reticulum Membrane ComplexEukaryotic translation initiation factor 2BcomplexEukaryotic translation initiation factor 3core complexExocystExomer complexFAR complexGeneral transcription factor complex TFIIDGeneral transcription factor complex TFIIHGET4-GET5 transmembrane domain recognition complexGID ubiquitin ligase complexGin4 complexGolgi transport complexGSE complexHMC complexHOPS complexINO80 chromatin remodeling complexLSM1-7 complexLsm1-7-Pat1 complexLSM2-7 complexLSM2-8 complexMCM complexMICOS complexMitochondrial electron transport complex IIIMitochondrial inner membrane pre-sequence translocasecomplexMitochondrial outer membrane translocase holocomplexMitochondrial proton-transporting ATP synthase complexMitochondrial pyruvate dehydrogenase complexNineTeen complexNOP8 60s ribosome pre-assembly complexNuA3 histone acetyltransferase complexNuA4 histone acetyltransferase complexNuclear condensin complexNuclear/nucleolar exosome complex, DIS3-RRP6 variantNuclear pore complexNucleolar ribonuclease MRP complexNucleolar ribonuclease P complexOligosaccharyl transferase complex variant 1Oligosaccharyl transferase complex variant 2PAF1 complexPAS complexPhosphatidylinositol 3-kinase complex IPolarisomePrefoldin complexPRP19-associated complexRad17 RFC-like complexRetromer complexRPD3L histone deacetylase complexRPD3S histone deacetylase complexRQC complexRSC complex variant RSC1RSC complex variant RSC2SAGA complexSCF-Met30 ubiquitin ligase complexSCF-Ufo1 ubiquitin ligase complexSEA complexSeptin complexSET3C histone deacetylase complexSignal recognition particleSLIK (SAGA-like) complexSmall ribosomal subunit processome, variant1Small ribosomal subunit processome, variant2Small ribosomal subunit processome, variant3SMC5-SMC6 SUMO ligase complexSm complexSpliceosomal commitment complexSPOTS complexSWI/SNF chromatin remodelling complexSwr1 chromatin remodelling complexTHO complexTranscription factor TFIIIC complexTranslocon complexTRAPPIII protein complexTRAPPII protein complexTRAPPI protein complexTREX complexU1 snRNPU2 snRNPU4 snRNPU4/U6 snRNPU4/U6.U5 tri-snRNP complexU5 snRNPU6 snRNP complexUTP-A complexUTP-B complexUTP-C complex variant 1UTP-C complex variant 2UTP-C complex variant 3Vacuolar proton translocating ATPase complex,Golgi variantVacuolar proton translocating ATPase complex,vacuole variantCellular Components••••••••••••••••actinbud neckcell peripherycytoplasmendosomeERGolgimitochondrionmixednuclear peripherynucleolusnucleusperoxisomespindle polevacuolar membranevacuole
Standard name
Human Ortholog
Description Subunit of the EGO/GSE complex; the vacuolar/endosomal membrane associated EGO/GSE complex regulates exit from rapamycin-induced growth arrest, stimulating microautophagy and sorting of Gap1p from the endosome to the plasma membrane; loss results in a defect in vacuolar acidification
Localization
Cell Percentages vacuolar membrane (96%)
Cell Cycle Regulation No
Subcompartmental Group vac memb-2

Meh1

Meh1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Meh1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available