ORF
Human Ortholog
Description Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; protein abundance increases in response to DNA replication stress; YKR018C has a paralog, IML2, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0.09 0 0 0 0 0 0 0 0
Cytoplasm 0.99 0.98 0.94 0.97 0.95 0.93 0.88 0.87 0.89 – 0.72 0.73 0.98 0.99 0.99 0.92 0.98 0.95 0.97 0.96 0.96 0.93 0.97 0.92
Endoplasmic Reticulum 0 0 0 0 0 0 0.08 0.07 0.05 – 0 0.06 0 0 0 0 0.05 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 – 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0.08 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 – 0.19 0.18 0 0 0 0 0 0.05 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 1 0 0 1 – 1 1 0 0 0 0 0 0 0 0 0 0 0 1
Bud 1 1 0 0 0 1 0 2 0 – 6 2 0 0 0 0 0 0 1 0 0 0 0 1
Bud Neck 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 5 3 9 1 6 8 12 6 10 – 2 8 3 0 2 5 0 0 0 0 0 0 0 0
Cytoplasm 325 245 440 221 270 467 566 420 312 – 193 329 247 294 272 49 63 21 317 239 486 71 174 164
Endoplasmic Reticulum 1 3 7 0 12 18 52 34 17 – 7 27 3 2 1 2 3 0 1 0 1 0 1 4
Endosome 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 1 0 4 0 0 0
Golgi 0 1 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 1 0 0 0
Mitochondria 0 0 0 0 0 1 2 0 5 – 13 10 0 0 1 0 0 0 0 0 5 0 0 0
Nucleus 2 1 2 2 3 3 18 17 13 – 9 11 4 0 3 4 1 0 0 2 3 1 1 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 1 0 0 0 0 1
Nucleolus 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 1 0 18 9 2 12 11 19 8 – 51 83 0 0 0 2 1 1 0 2 2 1 2 4
Unique Cell Count 328 250 466 229 284 500 644 481 350 268 450 252 296 276 53 64 22 326 248 509 77 181 180
Labelled Cell Count 335 254 476 233 293 511 661 498 366 282 471 257 296 279 62 68 22 326 248 509 77 181 180


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 14.4 18.4 12.4 11.7 12.7 11.6 12.2 12.2 10.8 – 9.2 11.2 15.9 16.9 16.6 25.2 23.6 21.0 13.6 14.0 16.3
Std Deviation (1e-4) 2.3 3.2 1.9 2.1 1.8 1.8 1.9 2.0 2.1 – 1.9 2.5 2.5 2.7 3.0 7.1 7.3 3.4 1.8 2.1 3.5
Intensity Change (Log2) – – – -0.08 0.04 -0.1 -0.03 -0.02 -0.2 – -0.44 -0.15 0.35 0.44 0.42 1.02 0.93 0.76 0.13 0.18 0.4


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Cell Periphery 0 0.2 -0.4 -0.1 -0.8 0.9 – 0 -0.2 0 0 0 0 0 0
Cytoplasm 1.2 0.4 -0.7 -3.7 -3.8 -2.8 – -8.5 -8.8 2.3 3.5 2.8 -0.6 1.4 0.2
Endoplasmic Reticulum 0 2.3 2.1 4.8 4.2 2.8 – 1.1 3.6 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Nucleus 0 0 0 2.9 3.4 3.5 – 0 2.6 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Vacuole 0 -2.6 -1.3 -2.2 0.1 -1.3 – 6.8 7.0 -3.2 -3.4 -3.3 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 6.8855 9.5334 7.5913 7.5279 7.5036 8.1355 9.7405 12.2972 11.4388 10.645 10.2145 11.4373 9.521 11.116 10.1795 10.1034 9.3956 10.3597
Actin 0.0076 0.0003 0.0017 0.0002 0.0013 0.001 0.026 0.001 0.0064 0.007 0.0002 0.0063 0.0142 0.001 0.0044 0.0001 0.0022 0.0006
Bud 0.0001 0.0001 0.0004 0.0001 0.0002 0.0002 0.0006 0.0003 0.0046 0.0002 0 0.0003 0.0008 0.0004 0.0006 0 0.0001 0.0001
Bud Neck 0.0015 0.0002 0.001 0.0002 0.0007 0.0008 0.0021 0.0003 0.0012 0.0007 0.0008 0.0024 0.0017 0.0002 0.0006 0.0003 0.0006 0.0013
Bud Periphery 0.0001 0 0.0005 0 0.0001 0.0001 0.0005 0.0001 0.0037 0.0001 0 0.0007 0.0009 0.0001 0.0006 0 0 0
Bud Site 0.0008 0.0005 0.0007 0.0001 0.0002 0.0001 0.0028 0.002 0.003 0.0003 0 0.0004 0.0029 0.0009 0.0023 0 0.0001 0
Cell Periphery 0.0001 0 0.0001 0 0 0 0.0002 0 0.0002 0 0 0 0.0002 0 0.0001 0 0 0
Cytoplasm 0.9298 0.983 0.9415 0.9752 0.9181 0.9583 0.8228 0.9706 0.943 0.9215 0.9704 0.9504 0.8996 0.9684 0.9376 0.9785 0.936 0.9523
Cytoplasmic Foci 0.0073 0.0007 0.0045 0.0015 0.0041 0.0051 0.0218 0.0035 0.006 0.0038 0.0016 0.0024 0.0113 0.0016 0.0048 0.0009 0.0121 0.0039
Eisosomes 0.0003 0 0 0 0 0 0.0002 0 0 0.0001 0 0 0.0002 0 0 0 0 0
Endoplasmic Reticulum 0.007 0.0024 0.0074 0.0056 0.0064 0.0043 0.0065 0.0018 0.0017 0.0036 0.0055 0.002 0.0079 0.0021 0.0027 0.0014 0.003 0.0034
Endosome 0.0051 0.0005 0.0083 0.0036 0.0349 0.007 0.0341 0.0005 0.002 0.0079 0.0007 0.0038 0.0087 0.0016 0.0107 0.0005 0.0024 0.0042
Golgi 0.0008 0 0.0006 0.0002 0.0013 0.0008 0.0136 0.0002 0.0015 0.0062 0 0.001 0.0024 0.0002 0.002 0 0.0028 0.0003
Lipid Particles 0.0012 0 0.0003 0.0001 0.0004 0.0001 0.0136 0.0001 0.0012 0.0057 0 0.0009 0.0015 0.0005 0.003 0 0.001 0.0001
Mitochondria 0.0004 0.0001 0.0018 0.0001 0.0021 0.0005 0.0028 0.0002 0.0008 0.0073 0.0001 0.0008 0.0033 0.0005 0.0055 0.0001 0.0003 0.0002
None 0.0078 0.0011 0.008 0.0013 0.0026 0.0021 0.0043 0.0017 0.0011 0.0014 0.0009 0.0011 0.0058 0.003 0.003 0.0018 0.0028 0.0016
Nuclear Periphery 0.0077 0.0021 0.0053 0.0038 0.0123 0.0038 0.0099 0.0017 0.0017 0.0034 0.0031 0.0023 0.0103 0.003 0.0027 0.0017 0.0027 0.0043
Nucleolus 0.0005 0 0.0002 0 0.0001 0 0.0005 0.0002 0.0001 0.0001 0 0.0002 0.0002 0.0001 0.0001 0 0 0
Nucleus 0.0121 0.0079 0.0114 0.0063 0.0103 0.0128 0.014 0.0082 0.012 0.0276 0.0154 0.0226 0.0218 0.0147 0.0143 0.0139 0.0197 0.0258
Peroxisomes 0.0051 0 0.0004 0 0.0002 0.0001 0.0116 0.0058 0.0072 0.0012 0 0.0007 0.0017 0.0002 0.0011 0 0.0128 0.0001
Punctate Nuclear 0.0033 0.0004 0.0024 0.0003 0.0018 0.0014 0.0091 0.0012 0.002 0.0012 0.0005 0.0008 0.0029 0.0008 0.0019 0.0003 0.0008 0.0009
Vacuole 0.0013 0.0005 0.0031 0.0011 0.0019 0.0011 0.0023 0.0003 0.0006 0.0007 0.0006 0.0008 0.0013 0.0005 0.0013 0.0003 0.0004 0.0006
Vacuole Periphery 0.0003 0.0001 0.0005 0.0002 0.001 0.0004 0.0008 0.0001 0.0001 0.0003 0.0001 0.0001 0.0005 0.0001 0.0005 0.0001 0.0001 0.0002

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 74.4722 56.6102 69.5639 67.5894 48.6067 88.102 69.9455 74.0192 90.6648 65.7731
Translational Efficiency 0.8182 0.8522 0.7783 0.9844 0.7953 0.6943 0.8372 0.6939 0.6474 0.6621

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
ORF
Human Ortholog
Description Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; protein abundance increases in response to DNA replication stress; YKR018C has a paralog, IML2, that arose from the whole genome duplication
Localization
Cell Percentages cytoplasm (99%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-7

YKR018C

YKR018C


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ykr018c-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available