Standard name | |
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Human Ortholog | |
Description | Component of the GARP (Golgi-associated retrograde protein) complex; GARP is required for the recycling of proteins from endosomes to the late Golgi, and for mitosis after DNA damage induced checkpoint arrest; links the (VFT/GARP) complex to the SNARE Tlg1p; members of the GARP complex are Vps51p-Vps52p-Vps53p-Vps54p |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 1 | 5 | 4 | 0 | 4 | 2 | – | 2 | 0 | 2 | 2 | 2 | 6 | 1 | 0 | 0 | 2 | 1 | 31 | 21 | 4 | 2 | 3 | 5 |
Bud | 17 | 6 | 41 | 16 | 19 | 29 | – | 52 | 46 | 52 | 29 | 32 | 4 | 2 | 3 | 3 | 11 | 1 | 3 | 2 | 0 | 0 | 0 | 0 |
Bud Neck | 3 | 0 | 7 | 0 | 1 | 0 | – | 3 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 3 | 1 | 0 | 0 | 0 | 0 |
Bud Site | 8 | 0 | 4 | 1 | 7 | 5 | – | 16 | 11 | 16 | 12 | 12 | 0 | 0 | 0 | 1 | 1 | 2 | – | – | – | – | – | – |
Cell Periphery | 3 | 3 | 0 | 3 | 1 | 1 | – | 0 | 3 | 3 | 1 | 3 | 1 | 0 | 0 | 0 | 2 | 4 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 30 | 22 | 29 | 18 | 29 | 13 | – | 35 | 14 | 21 | 14 | 11 | 6 | 6 | 10 | 48 | 52 | 59 | 1 | 2 | 1 | 0 | 0 | 0 |
Endoplasmic Reticulum | 1 | 12 | 3 | 2 | 5 | 4 | – | 2 | 3 | 3 | 3 | 6 | 8 | 3 | 1 | 6 | 4 | 6 | 0 | 1 | 3 | 0 | 0 | 0 |
Endosome | 213 | 92 | 2 | 3 | 45 | 5 | – | 18 | 9 | 10 | 5 | 8 | 36 | 23 | 66 | 147 | 106 | 101 | 267 | 182 | 142 | 34 | 41 | 44 |
Golgi | 0 | 49 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 1 | 12 | 8 | 6 | 24 | 12 | 11 | 107 | 157 | 24 | 25 | 32 | 44 |
Mitochondria | 449 | 304 | 314 | 247 | 249 | 509 | – | 694 | 499 | 542 | 414 | 410 | 48 | 55 | 45 | 36 | 35 | 25 | 133 | 80 | 75 | 11 | 17 | 19 |
Nucleus | 2 | 0 | 0 | 2 | 2 | 2 | – | 6 | 2 | 7 | 2 | 1 | 0 | 2 | 1 | 8 | 8 | 7 | 2 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 1 | 3 | – | 5 | 5 | 2 | 10 | 7 | 0 | 1 | 1 | 0 | 1 | 1 | 1 | 1 | 2 | 0 | 0 | 0 |
Nucleolus | 13 | 3 | 0 | 4 | 6 | 16 | – | 28 | 17 | 8 | 15 | 10 | 0 | 0 | 1 | 4 | 8 | 7 | 3 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 166 | 67 | 13 | 41 | 80 | 23 | – | 65 | 17 | 61 | 30 | 33 | 19 | 7 | 31 | 80 | 100 | 89 | 155 | 97 | 125 | 43 | 40 | 37 |
SpindlePole | 81 | 22 | 50 | 19 | 40 | 33 | – | 94 | 32 | 51 | 36 | 24 | 9 | 4 | 19 | 161 | 117 | 120 | 23 | 12 | 15 | 1 | 2 | 4 |
Vac/Vac Membrane | 21 | 16 | 3 | 0 | 3 | 6 | – | 7 | 6 | 6 | 3 | 7 | 3 | 2 | 4 | 19 | 13 | 13 | 14 | 12 | 6 | 0 | 2 | 0 |
Unique Cell Count | 668 | 468 | 370 | 276 | 367 | 556 | 811 | 542 | 624 | 454 | 458 | 109 | 94 | 146 | 353 | 314 | 302 | 756 | 579 | 414 | 123 | 142 | 160 | |
Labelled Cell Count | 1008 | 601 | 470 | 356 | 492 | 651 | 1027 | 664 | 785 | 576 | 567 | 152 | 114 | 188 | 538 | 472 | 447 | 756 | 579 | 414 | 123 | 142 | 160 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 4.9 | 5.9 | 3.7 | 3.8 | 4.6 | 3.8 | – | 4.0 | 3.8 | 4.2 | 4.0 | 4.2 | 5.8 | 5.8 | 5.7 | 7.9 | 7.6 | 7.7 | 5.4 | 5.9 | 6.0 |
Std Deviation (1e-4) | 0.7 | 0.9 | 0.7 | 1.1 | 1.3 | 0.9 | – | 1.0 | 1.1 | 1.1 | 1.0 | 1.2 | 1.1 | 1.2 | 1.0 | 2.1 | 2.2 | 2.4 | 1.0 | 1.2 | 1.1 |
Intensity Change (Log2) | – | – | – | 0.03 | 0.32 | 0.03 | – | 0.11 | 0.02 | 0.18 | 0.1 | 0.17 | 0.63 | 0.63 | 0.62 | 1.08 | 1.02 | 1.04 | 0.54 | 0.67 | 0.68 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | -2.3 | -2.7 | -3.3 | -5.8 | -3.7 | -5.7 |
Bud Neck | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | -0.6 | 0 | -3.9 | – | -2.5 | -3.7 | -3.1 | -3.1 | -3.6 | -0.8 | -0.5 | -0.4 | 2.5 | 3.5 | 4.5 |
Endoplasmic Reticulum | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 11.0 | 9.2 | 13.5 | 13.7 | 11.9 | 11.8 |
Golgi | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 5.1 | 3.8 | 0 |
Mitochondria | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | -8.7 | -5.7 | -12.0 | -20.1 | -19.2 | -19.8 |
Nucleus | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 5.1 | 0 | 6.5 | 7.7 | 9.9 | 9.3 |
SpindlePole | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | -1.5 | -2.5 | -0.2 | 9.5 | 7.2 | 7.8 |
Vacuole | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3.6 | 2.9 | 3.0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 1.0626 | 1.1981 | 1.2756 | 1.0832 | 1.2855 | 1.0349 | 0.6488 | 0.6733 | 0.8177 | 0.6603 | 0.8862 | 0.5485 | 2.0619 | 2.01 | 1.6753 | 1.3673 | 2.7996 | 2.2155 |
Actin | 0.0153 | 0.0041 | 0.0172 | 0.0178 | 0.0209 | 0.0065 | 0.0285 | 0.0071 | 0.0449 | 0.036 | 0.0131 | 0.0105 | 0.0763 | 0.0139 | 0.0294 | 0.0094 | 0.0854 | 0.0028 |
Bud | 0.0004 | 0.0071 | 0.0006 | 0.0043 | 0.0008 | 0.0005 | 0.0006 | 0.0049 | 0.0007 | 0.0015 | 0.0004 | 0.0089 | 0.0022 | 0.0029 | 0.0018 | 0.0001 | 0.0006 | 0.0001 |
Bud Neck | 0.0013 | 0.0006 | 0.0021 | 0.0032 | 0.0017 | 0.0019 | 0.0027 | 0.0006 | 0.0014 | 0.0002 | 0.0008 | 0.001 | 0.0049 | 0.0037 | 0.0005 | 0.0004 | 0.0024 | 0.0023 |
Bud Periphery | 0.0005 | 0.0026 | 0.0006 | 0.0046 | 0.001 | 0.0004 | 0.0006 | 0.0023 | 0.001 | 0.001 | 0.0005 | 0.0086 | 0.0029 | 0.0007 | 0.0011 | 0.0001 | 0.0007 | 0.0001 |
Bud Site | 0.0019 | 0.0111 | 0.0026 | 0.0025 | 0.0044 | 0.0005 | 0.0063 | 0.0058 | 0.006 | 0.0007 | 0.0009 | 0.0006 | 0.0165 | 0.0126 | 0.0026 | 0.0003 | 0.0028 | 0.0002 |
Cell Periphery | 0.0001 | 0.0002 | 0.0001 | 0.0003 | 0.0001 | 0.0001 | 0.0002 | 0.0002 | 0.0003 | 0.0002 | 0.0001 | 0.0004 | 0.0007 | 0.0003 | 0.0002 | 0 | 0.0004 | 0 |
Cytoplasm | 0.0047 | 0.0112 | 0.0081 | 0.0055 | 0.0198 | 0.0109 | 0.0264 | 0.0196 | 0.0062 | 0.0112 | 0.0026 | 0.0262 | 0.0148 | 0.0159 | 0.0153 | 0.0075 | 0.0215 | 0.0141 |
Cytoplasmic Foci | 0.1914 | 0.2674 | 0.2073 | 0.172 | 0.265 | 0.2105 | 0.2246 | 0.2693 | 0.2296 | 0.2656 | 0.2167 | 0.2512 | 0.2043 | 0.2489 | 0.2311 | 0.2064 | 0.1915 | 0.2492 |
Eisosomes | 0.0002 | 0 | 0.0001 | 0.0003 | 0.0002 | 0 | 0.0003 | 0.0001 | 0.0003 | 0.0002 | 0.0004 | 0.0001 | 0.0009 | 0.0002 | 0.0001 | 0 | 0.0003 | 0 |
Endoplasmic Reticulum | 0.0014 | 0.0007 | 0.0011 | 0.0004 | 0.0006 | 0.0006 | 0.0042 | 0.0015 | 0.0009 | 0.0008 | 0.0005 | 0.001 | 0.0061 | 0.0011 | 0.0017 | 0.001 | 0.0107 | 0.0004 |
Endosome | 0.2486 | 0.2742 | 0.2635 | 0.2487 | 0.1788 | 0.1887 | 0.2094 | 0.1884 | 0.1883 | 0.195 | 0.1359 | 0.138 | 0.1587 | 0.1905 | 0.1841 | 0.2815 | 0.3213 | 0.1906 |
Golgi | 0.135 | 0.1436 | 0.1621 | 0.1558 | 0.1148 | 0.1345 | 0.1024 | 0.119 | 0.1699 | 0.0824 | 0.1197 | 0.1025 | 0.1032 | 0.1448 | 0.1441 | 0.1413 | 0.1327 | 0.1848 |
Lipid Particles | 0.1226 | 0.0649 | 0.0867 | 0.0713 | 0.1147 | 0.1013 | 0.1561 | 0.0703 | 0.0638 | 0.0817 | 0.0493 | 0.1069 | 0.1318 | 0.0931 | 0.0801 | 0.0778 | 0.0531 | 0.1088 |
Mitochondria | 0.0244 | 0.0167 | 0.0237 | 0.0469 | 0.0467 | 0.0173 | 0.0172 | 0.0277 | 0.0376 | 0.0095 | 0.0571 | 0.0053 | 0.0299 | 0.0219 | 0.0273 | 0.0114 | 0.0082 | 0.0059 |
None | 0.0036 | 0.0039 | 0.0111 | 0.034 | 0.0127 | 0.0035 | 0.062 | 0.0224 | 0.0347 | 0.1258 | 0.0426 | 0.0262 | 0.0357 | 0.0091 | 0.022 | 0.0017 | 0.0067 | 0.027 |
Nuclear Periphery | 0.0072 | 0.0003 | 0.0015 | 0.0002 | 0.0017 | 0.0007 | 0.0047 | 0.0013 | 0.0005 | 0.0009 | 0.0008 | 0.0004 | 0.0222 | 0.0004 | 0.0034 | 0.0002 | 0.0358 | 0.0002 |
Nucleolus | 0.0005 | 0.0004 | 0.0003 | 0.0005 | 0.0009 | 0.0002 | 0.0006 | 0.0009 | 0.0004 | 0.0005 | 0.0021 | 0.0003 | 0.0018 | 0.0007 | 0.0008 | 0.0001 | 0.0007 | 0.0001 |
Nucleus | 0.0037 | 0.0006 | 0.0005 | 0.0003 | 0.0018 | 0.0003 | 0.0016 | 0.0008 | 0.0003 | 0.001 | 0.0008 | 0.0003 | 0.0074 | 0.0006 | 0.0033 | 0.0001 | 0.0152 | 0.0001 |
Peroxisomes | 0.2292 | 0.1785 | 0.2044 | 0.2254 | 0.2033 | 0.3165 | 0.1422 | 0.2474 | 0.2082 | 0.1784 | 0.3489 | 0.3074 | 0.1507 | 0.2335 | 0.2456 | 0.2569 | 0.0969 | 0.2103 |
Punctate Nuclear | 0.0032 | 0.0069 | 0.0028 | 0.0022 | 0.0063 | 0.0016 | 0.0044 | 0.0024 | 0.0015 | 0.0037 | 0.0042 | 0.0022 | 0.021 | 0.0021 | 0.0032 | 0.0007 | 0.0074 | 0.001 |
Vacuole | 0.0029 | 0.0037 | 0.0022 | 0.0022 | 0.0025 | 0.0022 | 0.0032 | 0.0067 | 0.0023 | 0.0029 | 0.0009 | 0.0014 | 0.0045 | 0.0022 | 0.0016 | 0.0021 | 0.0033 | 0.0012 |
Vacuole Periphery | 0.0018 | 0.0011 | 0.0014 | 0.0015 | 0.0014 | 0.0011 | 0.0018 | 0.0015 | 0.0012 | 0.001 | 0.0014 | 0.0006 | 0.0033 | 0.0009 | 0.0008 | 0.001 | 0.0024 | 0.0008 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 5.5727 | 11.9561 | 4.436 | 8.311 | 7.2253 | 6.6836 | 11.5851 | 14.2784 | 11.0024 | 10.1919 |
Translational Efficiency | 6.3729 | 1.7972 | 5.2528 | 3.3649 | 4.3741 | 4.7211 | 2.3154 | 2.7826 | 3.3377 | 3.3017 |
Dataset | Hit |
---|---|
Protein Concentration | ✔ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
1419 | 1551 | 303 | 1029 | 1882 | 1783 | 2221 | 680 | 3301 | 3334 | 2524 | 1709 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 709.98 | 750.76 | 991.34 | 900.82 | 608.10 | 785.35 | 870.72 | 879.44 | 651.90 | 769.26 | 885.20 | 892.31 |
Standard Deviation | 77.38 | 104.17 | 118.26 | 129.71 | 74.92 | 99.29 | 102.29 | 117.85 | 91.21 | 103.05 | 111.46 | 125.56 |
Intensity Change Log 2 | — | 0.080573 | 0.481602 | 0.343460 | — | 0.369027 | 0.517900 | 0.532277 | — | 0.220844 | 0.498462 | 0.433650 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.001100 | 0.002851 | 0.015223 | 0.016338 | 0.000656 | 0.002278 | 0.007177 | 0.019142 | 0.000847 | 0.002545 | 0.008143 | 0.017454 |
Bud Neck | 0.001567 | 0.001245 | 0.002806 | 0.003014 | 0.003489 | 0.000881 | 0.003981 | 0.002775 | 0.002663 | 0.001050 | 0.003840 | 0.002919 |
Bud Site | 0.001476 | 0.001609 | 0.013457 | 0.023226 | 0.001708 | 0.001921 | 0.009569 | 0.014866 | 0.001608 | 0.001776 | 0.010036 | 0.019900 |
Cell Periphery | 0.000056 | 0.000030 | 0.000075 | 0.000093 | 0.000056 | 0.000041 | 0.000040 | 0.000040 | 0.000056 | 0.000036 | 0.000044 | 0.000072 |
Cytoplasm | 0.006443 | 0.002916 | 0.001976 | 0.009102 | 0.006940 | 0.002061 | 0.011224 | 0.011999 | 0.006727 | 0.002459 | 0.010113 | 0.010255 |
Cytoplasmic Foci | 0.295952* | 0.316047* | 0.350947* | 0.300994* | 0.250492* | 0.280192* | 0.323025* | 0.315630* | 0.270034* | 0.296872* | 0.326377* | 0.306818* |
Eisosomes | 0.000119 | 0.000071 | 0.000069 | 0.000072 | 0.000109 | 0.000088 | 0.000061 | 0.000062 | 0.000113 | 0.000080 | 0.000062 | 0.000068 |
Endoplasmic Reticulum | 0.000112 | 0.000110 | 0.001983 | 0.001538 | 0.000196 | 0.000132 | 0.000818 | 0.000508 | 0.000160 | 0.000122 | 0.000958 | 0.001128 |
Endosome | 0.088151 | 0.148836 | 0.128626 | 0.203129* | 0.175286 | 0.163664 | 0.136240 | 0.171222 | 0.137829 | 0.156765 | 0.135326 | 0.190433 |
Golgi | 0.406447* | 0.370928* | 0.103222 | 0.083961 | 0.339648* | 0.389460* | 0.059644 | 0.078860 | 0.368363* | 0.380839* | 0.064875 | 0.081932 |
Lipid Particles | 0.027750 | 0.019703 | 0.033889 | 0.012397 | 0.064653 | 0.016429 | 0.036942 | 0.010680 | 0.048790 | 0.017952 | 0.036575 | 0.011714 |
Mitochondria | 0.032772 | 0.019682 | 0.001969 | 0.008756 | 0.050565 | 0.031344 | 0.009082 | 0.005857 | 0.042916 | 0.025919 | 0.008228 | 0.007602 |
Mitotic Spindle | 0.007007 | 0.017484 | 0.191310* | 0.122581* | 0.005835 | 0.009639 | 0.190154* | 0.080883 | 0.006339 | 0.013288 | 0.190293* | 0.105989* |
None | 0.000150 | 0.000384 | 0.000023 | 0.000047 | 0.000743 | 0.000043 | 0.000226 | 0.000184 | 0.000488 | 0.000202 | 0.000201 | 0.000101 |
Nuclear Periphery | 0.000079 | 0.000031 | 0.000333 | 0.000502 | 0.000072 | 0.000041 | 0.000292 | 0.000097 | 0.000075 | 0.000037 | 0.000297 | 0.000341 |
Nuclear Periphery Foci | 0.000420 | 0.000521 | 0.006545 | 0.002522 | 0.000925 | 0.000139 | 0.002179 | 0.000650 | 0.000708 | 0.000317 | 0.002703 | 0.001777 |
Nucleolus | 0.000309 | 0.000438 | 0.000776 | 0.000229 | 0.004637 | 0.000433 | 0.001515 | 0.000550 | 0.002777 | 0.000435 | 0.001427 | 0.000357 |
Nucleus | 0.000324 | 0.000238 | 0.000705 | 0.000493 | 0.002533 | 0.001144 | 0.002323 | 0.001195 | 0.001583 | 0.000723 | 0.002129 | 0.000772 |
Peroxisomes | 0.127184* | 0.094422 | 0.132383* | 0.171838* | 0.087449 | 0.095840 | 0.186511* | 0.263391* | 0.104530* | 0.095180 | 0.180013* | 0.208266* |
Vacuole | 0.001591 | 0.001688 | 0.012849 | 0.033558 | 0.002748 | 0.002597 | 0.018464 | 0.019276 | 0.002251 | 0.002174 | 0.017790 | 0.027875 |
Vacuole Periphery | 0.000990 | 0.000766 | 0.000835 | 0.005610 | 0.001260 | 0.001634 | 0.000532 | 0.002132 | 0.001144 | 0.001230 | 0.000568 | 0.004226 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -3.23 | -5.72 | -6.98 | -5.99 | -0.13 | -4.27 | -8.92 | -5.84 | -5.28 | -3.72 | -5.25 | -10.35 | -9.12 | -8.06 | -4.78 |
Bud Neck | 0.77 | -1.87 | -5.29 | -5.15 | -0.77 | 6.06 | -2.47 | 0.57 | -5.29 | 3.10 | 5.37 | -4.95 | -2.08 | -7.82 | 3.03 |
Bud Site | -0.29 | -3.99 | -10.06 | -9.93 | -2.87 | -0.44 | -8.60 | -7.47 | -7.26 | -3.04 | -0.50 | -9.67 | -12.36 | -12.15 | -6.20 |
Cell Periphery | 2.82 | -1.95 | -2.47 | -4.85 | -0.97 | 2.16 | 2.77 | 2.70 | -0.22 | 0.13 | 3.62 | 1.85 | -1.87 | -4.39 | -3.22 |
Cytoplasm | 3.01 | 2.35 | -2.90 | -5.72 | -4.85 | 5.42 | -4.94 | -3.34 | -5.62 | -0.43 | 5.91 | -4.95 | -4.40 | -8.21 | 0.00 |
Cytoplasmic Foci | -3.29 | -3.46 | 0.37 | 2.93 | 3.47 | -5.37 | -10.88 | -6.62 | -3.31 | 0.88 | -6.48 | -9.41 | -4.98 | -0.44 | 3.09 |
Eisosomes | 5.51 | 5.20 | 5.36 | -0.42 | -0.34 | 1.93 | 5.59 | 5.25 | 2.47 | 0.28 | 4.61 | 8.42 | 6.93 | 1.47 | -0.81 |
Endoplasmic Reticulum | 0.00 | -3.58 | -3.67 | -3.68 | 0.88 | 1.10 | -3.46 | -2.24 | -2.57 | 0.81 | 0.99 | -4.65 | -4.16 | -4.34 | -0.38 |
Endosome | -12.09 | -4.86 | -15.80 | -6.79 | -7.38 | 1.70 | 7.32 | 0.90 | -0.38 | -4.53 | -4.79 | 1.27 | -8.88 | -5.39 | -9.60 |
Golgi | 3.99 | 31.40 | 41.81* | 41.64* | 2.73 | -6.64 | 46.87* | 36.83* | 45.62* | -4.17 | -2.36 | 63.99* | 54.75* | 61.62* | -4.79 |
Lipid Particles | 2.84 | -1.22 | 5.93 | 3.01 | 4.08 | 13.03 | 6.55 | 14.22 | 2.38 | 10.08 | 12.56 | 3.72 | 15.24 | 3.69 | 11.40 |
Mitochondria | 4.54 | 13.04 | 9.55 | 5.12 | -4.29 | 5.26 | 14.27 | 14.25 | 9.44 | 1.99 | 7.07 | 17.32 | 17.03 | 10.51 | 0.72 |
Mitotic Spindle | -5.11 | -11.20 | -16.09 | -14.39 | 3.57 | -3.18 | -31.12* | -11.44 | -10.79 | 11.55 | -6.05 | -33.05 | -19.71 | -18.14 | 10.69 |
None | -1.03 | 3.66 | 2.86 | 1.44 | -2.31 | 6.84 | 3.66 | 3.67 | -1.57 | 0.43 | 2.54 | 2.64 | 5.07 | 0.71 | 1.47 |
Nuclear Periphery | 0.88 | -2.11 | -2.68 | -3.13 | -0.80 | 1.63 | -9.13 | -2.78 | -3.51 | 5.89 | 1.54 | -7.95 | -3.07 | -3.56 | 0.41 |
Nuclear Periphery Foci | -0.32 | -2.23 | -4.14 | -4.06 | 1.26 | 4.11 | -4.62 | -0.21 | -4.25 | 4.40 | 2.45 | -5.42 | -3.76 | -5.16 | 1.84 |
Nucleolus | -0.66 | -1.05 | -0.64 | 0.22 | 0.86 | 4.88 | 2.49 | 3.85 | -1.13 | 2.32 | 4.73 | 1.10 | 4.17 | -0.90 | 3.91 |
Nucleus | 0.35 | -1.62 | -2.61 | -2.63 | 0.66 | 1.77 | -1.87 | 0.65 | -0.78 | 2.13 | 1.91 | -3.35 | 0.90 | -0.96 | 3.96 |
Peroxisomes | 5.10 | -0.40 | -4.57 | -8.51 | -2.52 | -1.58 | -15.25 | -15.19 | -14.17 | -6.58 | 2.31 | -12.73 | -14.37 | -15.63 | -3.75 |
Vacuole | -0.51 | -4.34 | -12.97 | -12.91 | -5.56 | 0.30 | -14.25 | -8.33 | -8.32 | -0.55 | 0.06 | -15.17 | -15.29 | -15.24 | -4.66 |
Vacuole Periphery | 1.43 | 0.52 | -3.82 | -4.03 | -3.89 | -1.31 | 6.30 | -0.75 | -0.37 | -1.44 | -0.52 | 6.02 | -3.59 | -3.43 | -4.32 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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