Standard name | |
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Human Ortholog | |
Description | General amino acid permease; Gap1p senses the presence of amino acid substrates to regulate localization to the plasma membrane when needed; essential for invasive growth |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | – | 0 | 0 | 0 | – | 0 | 0 | 0 | – | 0 | 0 | 0 | – | – | – | 0 | 1 | 0 | 0 | 9 | 6 | 0 | 0 | 0 |
Bud | – | 0 | 0 | 0 | – | 0 | 1 | 0 | – | 1 | 0 | 0 | – | – | – | 1 | 0 | 0 | 0 | 2 | 1 | 0 | 0 | 0 |
Bud Neck | – | 0 | 0 | 0 | – | 0 | 0 | 0 | – | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | – | 0 | 0 | 0 | – | 0 | 0 | 0 | – | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | – | 0 | 0 | 1 | – | 0 | 0 | 0 | – | 0 | 1 | 0 | – | – | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | – | 31 | 20 | 11 | – | 20 | 50 | 52 | – | 93 | 62 | 62 | – | – | – | 7 | 7 | 3 | 0 | 20 | 8 | 0 | 0 | 0 |
Endoplasmic Reticulum | – | 17 | 6 | 3 | – | 3 | 1 | 2 | – | 3 | 1 | 5 | – | – | – | 7 | 1 | 1 | 0 | 19 | 25 | 0 | 15 | 5 |
Endosome | – | 0 | 0 | 0 | – | 0 | 0 | 0 | – | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 | 0 | 13 | 9 | 0 | 0 | 0 |
Golgi | – | 0 | 4 | 2 | – | 2 | 1 | 1 | – | 0 | 1 | 0 | – | – | – | 5 | 5 | 3 | 0 | 43 | 79 | 0 | 0 | 0 |
Mitochondria | – | 4 | 3 | 5 | – | 1 | 2 | 0 | – | 0 | 2 | 1 | – | – | – | 7 | 3 | 2 | 0 | 0 | 1 | 0 | 0 | 0 |
Nucleus | – | 1 | 4 | 0 | – | 1 | 3 | 1 | – | 2 | 3 | 0 | – | – | – | 7 | 6 | 1 | 0 | 14 | 5 | 0 | 0 | 0 |
Nuclear Periphery | – | 0 | 0 | 0 | – | 0 | 0 | 0 | – | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 |
Nucleolus | – | 40 | 39 | 23 | – | 18 | 22 | 24 | – | 11 | 17 | 6 | – | – | – | 24 | 5 | 4 | 0 | 4 | 1 | 0 | 0 | 0 |
Peroxisomes | – | 0 | 0 | 0 | – | 0 | 0 | 0 | – | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 | 0 | 3 | 3 | 0 | 0 | 0 |
SpindlePole | – | 0 | 0 | 0 | – | 0 | 0 | 0 | – | 0 | 0 | 0 | – | – | – | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | – | 5 | 20 | 3 | – | 14 | 21 | 9 | – | 5 | 4 | 1 | – | – | – | 61 | 20 | 13 | 2 | 66 | 36 | 0 | 1 | 0 |
Unique Cell Count | 90 | 84 | 41 | 53 | 89 | 86 | 112 | 85 | 73 | 100 | 43 | 22 | 5 | 212 | 193 | 1 | 17 | 7 | ||||||
Labelled Cell Count | 98 | 96 | 48 | 59 | 101 | 89 | 115 | 91 | 75 | 119 | 49 | 27 | 5 | 212 | 193 | 1 | 17 | 7 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | – | 219.2 | 200.9 | 202.3 | – | 250.1 | 302.2 | 268.6 | – | 343.5 | 312.2 | 350.8 | – | – | – | 120.0 | 94.0 | 174.7 | – | – | – |
Std Deviation (1e-4) | – | 56.4 | 58.7 | 67.9 | – | 89.7 | 98.2 | 105.5 | – | 97.2 | 99.7 | 95.2 | – | – | – | 52.2 | 57.7 | 64.5 | – | – | – |
Intensity Change (Log2) | – | – | – | 0.01 | – | 0.32 | 0.59 | 0.42 | – | 0.77 | 0.64 | 0.8 | – | – | – | -0.74 | -1.1 | -0.2 | – | – | – |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | – | 0 | 0 | 0 | – | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 |
Bud | 0 | – | 0 | 0 | 0 | – | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 |
Bud Neck | 0 | – | 0 | 0 | 0 | – | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 |
Bud Site | 0 | – | 0 | 0 | 0 | – | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 |
Cell Periphery | 0 | – | 0 | 0 | 0 | – | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 |
Cytoplasm | 0.4 | – | 1.7 | 4.3 | 4.8 | – | 8.3 | 6.4 | 7.6 | – | – | – | -3.2 | -1.0 | 0 |
Endoplasmic Reticulum | 0 | – | 0 | 0 | 0 | – | 0 | 0 | -0.1 | – | – | – | 0 | 0 | 0 |
Endosome | 0 | – | 0 | 0 | 0 | – | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 |
Golgi | 0 | – | 0 | 0 | 0 | – | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 |
Mitochondria | 0 | – | 0 | 0 | 0 | – | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 |
Nucleus | 0 | – | 0 | 0 | 0 | – | 0 | 0 | 0 | – | – | – | 0.6 | 0 | 0 |
Nuclear Periphery | 0 | – | 0 | 0 | 0 | – | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 |
Nucleolus | 1.0 | – | -1.4 | -3.0 | -2.5 | – | -5.8 | -3.6 | -5.3 | – | – | – | -3.2 | -3.9 | -2.4 |
Peroxisomes | 0 | – | 0 | 0 | 0 | – | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 |
SpindlePole | 0 | – | 0 | 0 | 0 | – | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 |
Vacuole | -2.2 | – | 0.3 | 0 | -2.3 | – | -4.0 | -3.6 | -4.1 | – | – | – | 5.1 | 2.6 | 3.2 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 14.8859 | 18.7686 | 19.0486 | 12.9947 | 10.2781 | 18.9405 | 14.7031 | 17.7536 | 13.563 | 9.5368 | 10.1705 | 17.5281 | 15.0466 | 18.4523 | 17.0799 | 13.5228 | 9.2288 | 16.3433 |
Actin | 0.0078 | 0.0001 | 0.0027 | 0.0166 | 0.0696 | 0.0001 | 0.0112 | 0.0005 | 0.0016 | 0.0174 | 0.0843 | 0.0001 | 0.0002 | 0 | 0.0275 | 0 | 0 | 0.0001 |
Bud | 0.0009 | 0.0004 | 0.001 | 0.0017 | 0.0044 | 0.0014 | 0.0006 | 0.0007 | 0.0004 | 0.0013 | 0.0008 | 0.0006 | 0 | 0.0003 | 0.0005 | 0.0002 | 0.0001 | 0.0003 |
Bud Neck | 0.0017 | 0.0008 | 0.0041 | 0.0008 | 0.003 | 0.0021 | 0.0013 | 0.0019 | 0.0013 | 0.002 | 0.0018 | 0.0011 | 0.0001 | 0.0015 | 0.0009 | 0.0006 | 0.0003 | 0.0018 |
Bud Periphery | 0.0015 | 0.0008 | 0.0017 | 0.003 | 0.0051 | 0.0027 | 0.0018 | 0.0021 | 0.0013 | 0.0046 | 0.0015 | 0.0022 | 0.0001 | 0.0009 | 0.0012 | 0.0004 | 0.0003 | 0.0013 |
Bud Site | 0.0033 | 0.0011 | 0.0185 | 0.0024 | 0.0043 | 0.0013 | 0.0151 | 0.0023 | 0.0015 | 0.0019 | 0.0063 | 0.0008 | 0 | 0.0011 | 0.0011 | 0.0004 | 0.0001 | 0.0007 |
Cell Periphery | 0.008 | 0.0087 | 0.0055 | 0.0039 | 0.0033 | 0.0068 | 0.0085 | 0.0179 | 0.0041 | 0.0071 | 0.0115 | 0.0082 | 0.0005 | 0.0191 | 0.0043 | 0.0026 | 0.0037 | 0.0043 |
Cytoplasm | 0.041 | 0.0161 | 0.004 | 0.0249 | 0.01 | 0.0019 | 0.0152 | 0.0083 | 0.0023 | 0.0036 | 0.0015 | 0.0039 | 0.0255 | 0.0009 | 0.092 | 0.0011 | 0.0002 | 0.0625 |
Cytoplasmic Foci | 0.0521 | 0.005 | 0.0103 | 0.033 | 0.089 | 0.009 | 0.0103 | 0.0082 | 0.0127 | 0.0202 | 0.0141 | 0.0022 | 0.0081 | 0.0028 | 0.0168 | 0.0031 | 0.002 | 0.0055 |
Eisosomes | 0.0001 | 0 | 0.0001 | 0.0002 | 0.0004 | 0 | 0.0002 | 0 | 0.0001 | 0.0002 | 0.0007 | 0 | 0.0002 | 0 | 0.0001 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0.0103 | 0.0047 | 0.0051 | 0.0126 | 0.0028 | 0.0029 | 0.0721 | 0.0283 | 0.0107 | 0.0032 | 0.0039 | 0.0543 | 0.005 | 0.0011 | 0.0219 | 0.0005 | 0.0005 | 0.0202 |
Endosome | 0.1202 | 0.0803 | 0.0952 | 0.1134 | 0.0739 | 0.0572 | 0.1067 | 0.0514 | 0.1302 | 0.1221 | 0.118 | 0.0886 | 0.3001 | 0.0732 | 0.0659 | 0.1122 | 0.0588 | 0.0763 |
Golgi | 0.0204 | 0.0089 | 0.0104 | 0.0048 | 0.0147 | 0.0056 | 0.016 | 0.0066 | 0.0215 | 0.0103 | 0.0543 | 0.012 | 0.0065 | 0.0034 | 0.0301 | 0.0045 | 0.0035 | 0.0086 |
Lipid Particles | 0.045 | 0.0101 | 0.0121 | 0.0213 | 0.0291 | 0.0153 | 0.0271 | 0.0333 | 0.024 | 0.0341 | 0.0299 | 0.0223 | 0.0022 | 0.0397 | 0.0223 | 0.0039 | 0.0918 | 0.0496 |
Mitochondria | 0.0211 | 0.0049 | 0.0292 | 0.011 | 0.0066 | 0.0021 | 0.0198 | 0.0031 | 0.0302 | 0.0113 | 0.0118 | 0.0111 | 0.0007 | 0.0353 | 0.1157 | 0.0007 | 0.0005 | 0.0104 |
None | 0.0081 | 0.0001 | 0.0002 | 0.0051 | 0.0307 | 0.0001 | 0.0052 | 0.0002 | 0.0002 | 0.0025 | 0.0006 | 0.0001 | 0.2923 | 0.0001 | 0.0003 | 0 | 0 | 0.0003 |
Nuclear Periphery | 0.0421 | 0.0161 | 0.0155 | 0.01 | 0.0041 | 0.0048 | 0.0555 | 0.011 | 0.0119 | 0.0041 | 0.0037 | 0.046 | 0.0055 | 0.0035 | 0.0089 | 0.001 | 0.009 | 0.0156 |
Nucleolus | 0.0075 | 0.0055 | 0.0158 | 0.0157 | 0.0024 | 0.009 | 0.0027 | 0.0088 | 0.0019 | 0.0044 | 0.0097 | 0.0045 | 0.0011 | 0.0097 | 0.0022 | 0.0022 | 0.0024 | 0.0201 |
Nucleus | 0.0354 | 0.0072 | 0.0126 | 0.0073 | 0.0025 | 0.0059 | 0.0081 | 0.006 | 0.0024 | 0.0049 | 0.0033 | 0.0046 | 0.0053 | 0.0038 | 0.017 | 0.0016 | 0.0019 | 0.0391 |
Peroxisomes | 0.0042 | 0.0002 | 0.0032 | 0.0008 | 0.0137 | 0.0008 | 0.0084 | 0.0002 | 0.0082 | 0.0037 | 0.0054 | 0.0002 | 0.0001 | 0.0004 | 0.0568 | 0.0002 | 0.0001 | 0.0009 |
Punctate Nuclear | 0.0544 | 0.0001 | 0.0003 | 0.0021 | 0.0117 | 0.0005 | 0.0011 | 0.0001 | 0.0003 | 0.0011 | 0.0005 | 0.0001 | 0.0061 | 0.0001 | 0.0005 | 0 | 0.0001 | 0.0022 |
Vacuole | 0.333 | 0.5948 | 0.495 | 0.5188 | 0.4619 | 0.6419 | 0.4653 | 0.6142 | 0.4931 | 0.565 | 0.5074 | 0.5635 | 0.1554 | 0.4673 | 0.284 | 0.6199 | 0.5668 | 0.4065 |
Vacuole Periphery | 0.1819 | 0.2343 | 0.2577 | 0.1905 | 0.157 | 0.2286 | 0.1478 | 0.1949 | 0.2402 | 0.1749 | 0.1292 | 0.1735 | 0.1847 | 0.3357 | 0.23 | 0.2447 | 0.2578 | 0.2739 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 53.6758 | 41.4398 | 31.9639 | 33.6739 | 25.702 | 17.0692 | 14.4719 | 15.8142 | 20.88 | 18.257 |
Translational Efficiency | 1.3635 | 1.0457 | 0.9591 | 1.123 | 1.2481 | 0.6845 | 1.0682 | 0.7465 | 0.8667 | 0.836 |
Dataset | Hit |
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Protein Concentration | ✔ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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