Standard name | |
---|---|
Human Ortholog | |
Description | Translation initiation factor eIF4A; DEA(D/H)-box RNA helicase that couples ATPase activity to RNA binding and unwinding; forms a dumbbell structure of two compact domains connected by a linker; interacts with eIF4G; protein abundance increases in response to DNA replication stress; TIF1 has a paralog, TIF2, that arose from the whole genome duplication |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 1 | 7 | 13 | 11 | 26 | 26 | 48 | 28 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 1 | 2 | 6 | 2 | 1 | 1 | 6 | 3 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 3 | 1 | 6 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 2 | 6 | 10 | 4 | 4 | 12 | 20 | 27 | 31 | 26 | 55 | 47 | 5 | 14 | 15 | 6 | 9 | 5 | 0 | 0 | 1 | 0 | 0 | 0 |
Cytoplasm | 162 | 321 | 357 | 318 | 321 | 287 | 376 | 318 | 227 | 283 | 236 | 256 | 231 | 375 | 501 | 266 | 380 | 184 | 153 | 311 | 340 | 238 | 223 | 277 |
Endoplasmic Reticulum | 0 | 1 | 5 | 9 | 13 | 25 | 73 | 68 | 61 | 93 | 59 | 91 | 2 | 3 | 2 | 13 | 12 | 6 | 2 | 1 | 5 | 0 | 1 | 4 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 2 | 5 | 8 | 6 | 6 | 12 | 8 | 0 | 1 | 0 | 4 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 1 | 0 | 0 | 1 | 1 | 11 | 28 | 16 | 32 | 29 | 38 | 44 | 0 | 0 | 0 | 1 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 2 | 1 | 0 | 1 | 1 | 1 | 1 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 0 | 0 | 1 | 0 | 1 | 1 | 0 | 1 | 2 | 0 | 5 | 2 | 2 | 1 | 0 | 2 | 3 | 2 | 0 | 0 | 3 | 1 | 3 | 0 |
Unique Cell Count | 163 | 322 | 364 | 328 | 338 | 330 | 486 | 421 | 336 | 424 | 380 | 421 | 235 | 382 | 505 | 273 | 387 | 186 | 157 | 319 | 355 | 248 | 232 | 292 |
Labelled Cell Count | 165 | 328 | 373 | 332 | 342 | 350 | 522 | 451 | 387 | 466 | 460 | 482 | 240 | 394 | 520 | 292 | 408 | 198 | 157 | 319 | 355 | 248 | 232 | 292 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 69.3 | 97.4 | 74.8 | 63.6 | 53.4 | 43.4 | 40.5 | 41.1 | 36.9 | 36.7 | 34.7 | 35.0 | 83.8 | 78.8 | 72.6 | 103.0 | 93.0 | 103.8 | 97.1 | 96.7 | 104.1 |
Std Deviation (1e-4) | 14.4 | 18.9 | 15.9 | 14.8 | 12.8 | 11.4 | 10.7 | 10.4 | 9.7 | 8.9 | 9.0 | 8.3 | 19.5 | 15.4 | 16.4 | 34.5 | 34.7 | 38.0 | 27.0 | 27.6 | 26.0 |
Intensity Change (Log2) | – | – | – | -0.23 | -0.48 | -0.78 | -0.89 | -0.86 | -1.02 | -1.03 | -1.11 | -1.09 | 0.16 | 0.08 | -0.04 | 0.46 | 0.32 | 0.47 | 0.38 | 0.37 | 0.48 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 3.1 | 3.1 | 5.4 | 4.8 | 7.0 | 5.0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | -1.4 | -1.5 | 0.7 | 1.1 | 2.4 | 3.6 | 2.3 | 5.7 | 4.5 | -0.5 | 0.7 | 0.2 | -0.4 | -0.4 | 0 |
Cytoplasm | -1.0 | -2.3 | -5.7 | -8.7 | -9.1 | -10.8 | -11.2 | -12.2 | -12.6 | 0.2 | 0.1 | 1.5 | -0.5 | 0.1 | 0.7 |
Endoplasmic Reticulum | 1.3 | 2.1 | 4.0 | 6.8 | 7.1 | 7.6 | 8.7 | 6.9 | 8.6 | 0 | 0 | 0 | 2.6 | 1.6 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3.4 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 3.5 | 4.7 | 3.8 | 6.0 | 5.1 | 6.2 | 6.3 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 59.948 | 85.5053 | 75.4831 | 75.7662 | 73.7266 | 73.9282 | 48.4097 | 94.6967 | 79.3715 | 74.2537 | 74.6811 | 80.8133 | 78.1878 | 98.9593 | 91.9049 | 84.5921 | 82.9739 | 91.5759 |
Actin | 0.0466 | 0.0003 | 0.014 | 0.0002 | 0.0016 | 0.0014 | 0.0145 | 0.002 | 0.0178 | 0.0097 | 0.0005 | 0.0032 | 0.0075 | 0.0001 | 0.0002 | 0.0009 | 0.0002 | 0.0003 |
Bud | 0.0009 | 0.0003 | 0.0007 | 0.0008 | 0.0003 | 0.0009 | 0.0032 | 0.0005 | 0.0017 | 0.0021 | 0.0014 | 0.0015 | 0.0013 | 0.0002 | 0.0007 | 0.0003 | 0.0017 | 0.0004 |
Bud Neck | 0.001 | 0.0003 | 0.0016 | 0.0009 | 0.0002 | 0.0013 | 0.0016 | 0.0006 | 0.001 | 0.0009 | 0.0009 | 0.0018 | 0.0007 | 0.0003 | 0.0006 | 0.0006 | 0.0008 | 0.0018 |
Bud Periphery | 0.0008 | 0.0001 | 0.0004 | 0.0005 | 0.0001 | 0.0007 | 0.0039 | 0.0004 | 0.0008 | 0.0012 | 0.0005 | 0.0021 | 0.0015 | 0.0001 | 0.001 | 0.0002 | 0.0017 | 0.0003 |
Bud Site | 0.0017 | 0.0003 | 0.0077 | 0.0002 | 0.0001 | 0.0001 | 0.0092 | 0.0013 | 0.0061 | 0.0035 | 0.0006 | 0.0006 | 0.001 | 0.0002 | 0.0003 | 0.0003 | 0.0002 | 0.0001 |
Cell Periphery | 0.0057 | 0.0039 | 0.0096 | 0.0018 | 0.0002 | 0.002 | 0.0058 | 0.0117 | 0.0073 | 0.0012 | 0.006 | 0.0047 | 0.0071 | 0.0042 | 0.0062 | 0.0021 | 0.0014 | 0.0011 |
Cytoplasm | 0.6558 | 0.9585 | 0.8349 | 0.827 | 0.8821 | 0.8571 | 0.6518 | 0.9338 | 0.8308 | 0.7883 | 0.8177 | 0.797 | 0.7715 | 0.9456 | 0.8887 | 0.7967 | 0.8571 | 0.8518 |
Cytoplasmic Foci | 0.036 | 0.0009 | 0.0107 | 0.0023 | 0.0024 | 0.0055 | 0.0318 | 0.0031 | 0.0101 | 0.0199 | 0.0112 | 0.0043 | 0.0082 | 0.0006 | 0.0013 | 0.0091 | 0.0109 | 0.0038 |
Eisosomes | 0.0017 | 0 | 0.0005 | 0 | 0 | 0 | 0.0008 | 0.0001 | 0.0004 | 0.0008 | 0.0001 | 0.0003 | 0.0002 | 0 | 0.0001 | 0.0011 | 0.0002 | 0 |
Endoplasmic Reticulum | 0.0586 | 0.0102 | 0.0158 | 0.0132 | 0.0018 | 0.021 | 0.0388 | 0.0054 | 0.0133 | 0.0032 | 0.0028 | 0.0123 | 0.0636 | 0.0186 | 0.034 | 0.0152 | 0.0285 | 0.09 |
Endosome | 0.013 | 0.0017 | 0.0093 | 0.0096 | 0.0061 | 0.0247 | 0.0387 | 0.0018 | 0.0231 | 0.0151 | 0.0096 | 0.025 | 0.0072 | 0.001 | 0.0025 | 0.011 | 0.0114 | 0.0093 |
Golgi | 0.0041 | 0.0002 | 0.003 | 0.0004 | 0.0002 | 0.0118 | 0.0069 | 0.0009 | 0.0093 | 0.023 | 0.0004 | 0.0052 | 0.0013 | 0.0001 | 0.0004 | 0.0013 | 0.0012 | 0.0014 |
Lipid Particles | 0.0132 | 0.0005 | 0.0074 | 0.0004 | 0.0001 | 0.0004 | 0.0122 | 0.0024 | 0.0034 | 0.0039 | 0.0042 | 0.001 | 0.0033 | 0.0006 | 0.0015 | 0.0121 | 0.0025 | 0.0005 |
Mitochondria | 0.008 | 0.0003 | 0.0034 | 0.0022 | 0.0011 | 0.0206 | 0.0141 | 0.0012 | 0.002 | 0.0324 | 0.0012 | 0.0583 | 0.0076 | 0.0001 | 0.0069 | 0.0091 | 0.0079 | 0.0016 |
None | 0.0144 | 0.0001 | 0.0029 | 0.0003 | 0.001 | 0.0004 | 0.022 | 0.0003 | 0.0049 | 0.0148 | 0.0122 | 0.001 | 0.0066 | 0.0001 | 0.0021 | 0.0105 | 0.0068 | 0.0012 |
Nuclear Periphery | 0.021 | 0.0021 | 0.0037 | 0.0039 | 0.029 | 0.0049 | 0.0337 | 0.0024 | 0.0042 | 0.0037 | 0.0034 | 0.0389 | 0.0161 | 0.0019 | 0.0045 | 0.0118 | 0.0025 | 0.0051 |
Nucleolus | 0.0092 | 0 | 0.0036 | 0.0001 | 0.0011 | 0.0002 | 0.0032 | 0.0002 | 0.0024 | 0.0028 | 0.0015 | 0.0011 | 0.0038 | 0 | 0.0013 | 0.0057 | 0.0119 | 0.0002 |
Nucleus | 0.0161 | 0.0011 | 0.0026 | 0.0025 | 0.0443 | 0.0028 | 0.02 | 0.002 | 0.004 | 0.0043 | 0.0028 | 0.0126 | 0.0099 | 0.001 | 0.0029 | 0.0032 | 0.0092 | 0.0017 |
Peroxisomes | 0.0065 | 0 | 0.0033 | 0.0001 | 0.0001 | 0.0001 | 0.0015 | 0.0003 | 0.0007 | 0.0021 | 0.0002 | 0.0002 | 0.0011 | 0 | 0.0001 | 0.0011 | 0.0004 | 0.0001 |
Punctate Nuclear | 0.0159 | 0 | 0.0049 | 0 | 0.006 | 0.0001 | 0.0032 | 0.0002 | 0.0006 | 0.0008 | 0.0004 | 0.0003 | 0.0057 | 0 | 0.0001 | 0.0025 | 0.0006 | 0.0001 |
Vacuole | 0.0525 | 0.0179 | 0.0529 | 0.1153 | 0.0192 | 0.0354 | 0.0716 | 0.0279 | 0.0509 | 0.0576 | 0.1062 | 0.0155 | 0.0635 | 0.0244 | 0.0405 | 0.0955 | 0.0327 | 0.022 |
Vacuole Periphery | 0.0174 | 0.0014 | 0.007 | 0.0183 | 0.0028 | 0.0085 | 0.0114 | 0.0016 | 0.0051 | 0.0088 | 0.0165 | 0.0132 | 0.0112 | 0.0009 | 0.004 | 0.0097 | 0.0102 | 0.0072 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 1836.221 | 1792.5809 | 1790.4034 | 1564.4865 | 1445.6648 | 1578.0716 | 1590.8492 | 1370.6169 | 1328.2719 | 1375.5069 |
Translational Efficiency | 0.796 | 0.8362 | 0.7799 | 0.862 | 0.8911 | 0.9121 | 0.8566 | 0.9356 | 0.8468 | 0.8545 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
1397 | 248 | 240 | 819 | 2071 | 1112 | 187 | 463 | 3468 | 1360 | 427 | 1282 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 4472.54 | 5944.49 | 6078.33 | 7245.03 | 4998.57 | 5229.26 | 7126.30 | 7848.72 | 4786.67 | 5359.68 | 6537.28 | 7463.06 |
Standard Deviation | 1301.86 | 1145.17 | 1163.66 | 1729.82 | 1022.74 | 1054.52 | 1393.23 | 1555.12 | 1172.15 | 1106.64 | 1371.67 | 1693.84 |
Intensity Change Log 2 | — | 0.410459 | 0.442581 | 0.695897 | — | 0.065091 | 0.511638 | 0.650942 | — | 0.238508 | 0.479438 | 0.672346 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000046 | 0.000204 | 0.000136 | 0.000331 | 0.000040 | 0.000135 | 0.000247 | 0.000262 | 0.000043 | 0.000147 | 0.000185 | 0.000306 |
Bud Neck | 0.000236 | 0.001165 | 0.005386 | 0.003265 | 0.000273 | 0.000802 | 0.005500 | 0.002216 | 0.000258 | 0.000868 | 0.005436 | 0.002886 |
Bud Site | 0.002445 | 0.009399 | 0.005774 | 0.012834 | 0.002769 | 0.003492 | 0.014794 | 0.009911 | 0.002638 | 0.004569 | 0.009724 | 0.011778 |
Cell Periphery | 0.014310 | 0.055221 | 0.027497 | 0.064398 | 0.007907 | 0.014791 | 0.030519 | 0.097885 | 0.010486 | 0.022163 | 0.028820 | 0.076492 |
Cytoplasm | 0.811097* | 0.596700* | 0.336263* | 0.469181* | 0.809649* | 0.742388* | 0.439022* | 0.359719* | 0.810233* | 0.715821* | 0.381265* | 0.429648* |
Cytoplasmic Foci | 0.006619 | 0.016828 | 0.014763 | 0.006538 | 0.011752 | 0.011718 | 0.014758 | 0.003698 | 0.009684 | 0.012650 | 0.014761 | 0.005512 |
Eisosomes | 0.000001 | 0.000005 | 0.000005 | 0.000007 | 0.000001 | 0.000002 | 0.000007 | 0.000009 | 0.000001 | 0.000003 | 0.000006 | 0.000008 |
Endoplasmic Reticulum | 0.000893 | 0.000780 | 0.000278 | 0.000325 | 0.001128 | 0.000728 | 0.000303 | 0.000280 | 0.001033 | 0.000738 | 0.000289 | 0.000309 |
Endosome | 0.000465 | 0.001289 | 0.001510 | 0.002504 | 0.000623 | 0.000776 | 0.003141 | 0.000517 | 0.000560 | 0.000870 | 0.002224 | 0.001786 |
Golgi | 0.038090 | 0.074694 | 0.033775 | 0.013555 | 0.046607 | 0.055215 | 0.025205 | 0.011384 | 0.043176 | 0.058767 | 0.030022 | 0.012771 |
Lipid Particles | 0.010679 | 0.036867 | 0.038254 | 0.036535 | 0.008005 | 0.014003 | 0.054473 | 0.062822 | 0.009082 | 0.018172 | 0.045357 | 0.046029 |
Mitochondria | 0.000115 | 0.000405 | 0.000074 | 0.001946 | 0.000235 | 0.000464 | 0.000353 | 0.000162 | 0.000186 | 0.000453 | 0.000196 | 0.001302 |
Mitotic Spindle | 0.000524 | 0.001846 | 0.003496 | 0.001231 | 0.000999 | 0.001616 | 0.004965 | 0.000390 | 0.000808 | 0.001658 | 0.004139 | 0.000927 |
None | 0.004638 | 0.001380 | 0.000646 | 0.001095 | 0.002295 | 0.001816 | 0.000751 | 0.000412 | 0.003239 | 0.001736 | 0.000692 | 0.000848 |
Nuclear Periphery | 0.000367 | 0.000774 | 0.001334 | 0.000546 | 0.000393 | 0.000513 | 0.001365 | 0.000993 | 0.000383 | 0.000561 | 0.001348 | 0.000707 |
Nuclear Periphery Foci | 0.008528 | 0.011603 | 0.015777 | 0.006039 | 0.008803 | 0.011099 | 0.014150 | 0.006865 | 0.008692 | 0.011191 | 0.015065 | 0.006337 |
Nucleolus | 0.000079 | 0.000073 | 0.000128 | 0.000280 | 0.000043 | 0.000171 | 0.000125 | 0.000175 | 0.000057 | 0.000153 | 0.000126 | 0.000242 |
Nucleus | 0.008670 | 0.007484 | 0.014869 | 0.011197 | 0.003700 | 0.011806 | 0.020494 | 0.006832 | 0.005702 | 0.011018 | 0.017333 | 0.009621 |
Peroxisomes | 0.000031 | 0.000064 | 0.000032 | 0.000165 | 0.000020 | 0.000029 | 0.000059 | 0.000107 | 0.000024 | 0.000035 | 0.000044 | 0.000144 |
Vacuole | 0.051030 | 0.123362 | 0.457961* | 0.339125* | 0.036074 | 0.083002 | 0.341144* | 0.415120* | 0.042098 | 0.090362 | 0.406802* | 0.366571* |
Vacuole Periphery | 0.041136 | 0.059855 | 0.042043 | 0.028903 | 0.058687 | 0.045436 | 0.028625 | 0.020243 | 0.051617 | 0.048066 | 0.036167 | 0.025775 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -3.27 | -6.77 | -5.40 | -2.13 | -2.98 | -2.86 | -6.09 | -3.38 | -1.58 | 0.61 | -3.65 | -8.64 | -6.40 | -3.24 | -1.85 |
Bud Neck | -4.90 | -7.36 | -5.69 | -4.01 | 3.22 | -5.30 | -6.91 | -4.87 | -3.44 | 4.76 | -7.35 | -10.08 | -6.97 | -5.40 | 5.19 |
Bud Site | -5.96 | -4.04 | -5.65 | -2.24 | -3.40 | -1.54 | -2.73 | -3.43 | -3.00 | 1.18 | -4.34 | -3.68 | -6.51 | -5.16 | -0.86 |
Cell Periphery | -7.33 | -2.79 | -11.07 | -0.11 | -6.52 | -5.10 | -7.26 | -15.44 | -13.88 | -10.45 | -7.36 | -6.76 | -18.88 | -13.80 | -11.39 |
Cytoplasm | 9.22 | 21.37 | 24.19 | 4.92 | -5.56 | 6.50 | 15.44 | 26.51 | 20.37 | 2.32 | 9.96 | 26.11 | 35.18 | 21.26 | -2.97 |
Cytoplasmic Foci | -4.85 | -4.79 | -0.34 | 4.27 | 4.33 | -0.42 | -2.44 | 7.32 | 6.87 | 5.85 | -3.62 | -4.81 | 4.34 | 6.52 | 6.66 |
Eisosomes | -4.67 | -7.42 | -8.16 | -2.19 | -1.13 | -4.20 | -6.99 | -8.60 | -7.10 | 0.09 | -5.99 | -10.04 | -11.67 | -8.24 | -0.54 |
Endoplasmic Reticulum | 0.77 | 5.63 | 5.47 | 3.76 | -0.62 | 2.17 | 8.35 | 8.36 | 2.83 | 0.10 | 1.99 | 10.21 | 10.15 | 3.35 | -0.41 |
Endosome | -3.16 | -8.16 | -2.80 | -1.39 | -0.90 | -2.20 | -1.44 | 1.24 | 2.72 | 1.53 | -4.15 | -2.47 | -2.58 | -1.70 | 0.64 |
Golgi | -5.55 | 2.63 | 10.90 | 9.81 | 6.29 | -2.30 | 8.03 | 15.61 | 14.35 | 4.47 | -5.07 | 7.48 | 18.39 | 16.93 | 7.55 |
Lipid Particles | -6.70 | -7.72 | -9.96 | 0.48 | 0.78 | -4.68 | -7.25 | -12.72 | -11.00 | -1.19 | -7.12 | -10.51 | -16.24 | -10.80 | -0.00 |
Mitochondria | -1.07 | 1.58 | -1.36 | -1.10 | -1.38 | -0.99 | -0.29 | 0.90 | 1.27 | 0.67 | -1.37 | 0.11 | -1.28 | -0.91 | -1.29 |
Mitotic Spindle | -5.89 | -2.04 | -2.17 | 1.57 | 1.62 | -2.33 | -1.48 | 4.69 | 4.67 | 1.76 | -3.91 | -2.44 | -0.51 | 2.55 | 2.34 |
None | 3.70 | 4.57 | 3.83 | 0.73 | -2.17 | 0.77 | 2.51 | 3.00 | 9.21 | 2.84 | 2.88 | 4.99 | 4.37 | 4.27 | -1.42 |
Nuclear Periphery | -4.79 | -6.92 | -3.33 | 2.76 | 5.81 | -2.20 | -6.02 | -2.30 | -1.77 | 1.77 | -4.16 | -9.06 | -3.17 | -1.16 | 5.37 |
Nuclear Periphery Foci | -2.50 | -5.42 | 3.24 | 4.69 | 7.47 | -2.62 | -3.37 | 2.83 | 5.41 | 4.98 | -3.50 | -6.25 | 4.38 | 7.16 | 8.93 |
Nucleolus | 0.24 | -3.22 | -3.77 | -3.82 | -2.42 | -1.29 | -7.76 | -4.98 | -0.03 | -1.15 | -1.19 | -6.38 | -5.00 | -1.26 | -2.67 |
Nucleus | 0.32 | -3.90 | -2.97 | -3.12 | 2.03 | -7.51 | -5.89 | -3.05 | 1.11 | 3.83 | -5.71 | -7.27 | -5.24 | -0.75 | 4.00 |
Peroxisomes | -2.11 | -0.70 | -3.01 | -2.01 | -2.82 | -1.00 | -2.47 | -5.86 | -4.34 | -2.31 | -1.36 | -2.65 | -4.22 | -3.61 | -3.27 |
Vacuole | -6.23 | -19.84 | -28.13 | -14.19 | 4.92 | -9.95 | -15.17 | -28.47 | -23.35 | -2.75 | -10.95 | -24.81 | -40.06 | -30.00 | 2.30 |
Vacuole Periphery | -2.78 | 1.16 | 4.94 | 5.26 | 2.26 | 3.78 | 8.87 | 12.40 | 7.73 | 1.48 | 1.40 | 6.66 | 12.23 | 8.40 | 2.50 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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