Standard name | |
---|---|
Human Ortholog | |
Description | Negative regulatory subunit of protein phosphatase 1 (Ppz1p); involved in coenzyme A biosynthesis; subunit of phosphopantothenoylcysteine decarboxylase (PPCDC: Cab3p, Sis2p, Vhs3p) complex and the CoA-Synthesizing Protein Complex (CoA-SPC: Cab2p, Cab3p, Cab4p, Cab5p, Sis2p and Vhs3p); SIS2 has a paralog, VHS3, that arose from the whole genome duplication |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 2 | 3 | 0 | 0 | 4 | 15 | 16 | 18 | 14 | 19 | 13 | 32 | 0 | 3 | 2 | 0 | 1 | 2 | 5 | 3 | 6 | 1 | 3 | 9 |
Bud | 2 | 4 | 1 | 0 | 1 | 7 | 11 | 16 | 6 | 10 | 14 | 5 | 2 | 0 | 0 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 5 | 4 | 6 | 1 | 5 | 5 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 125 | 124 | 72 | 134 | 134 | 112 | 93 | 73 | 42 | 70 | 82 | 114 | 97 | 125 | 148 | 145 | 80 | 137 | 29 | 8 | 15 | 7 | 14 | 34 |
Cytoplasm | 280 | 264 | 144 | 196 | 181 | 259 | 306 | 391 | 210 | 277 | 278 | 279 | 212 | 252 | 264 | 255 | 216 | 262 | 219 | 262 | 130 | 32 | 97 | 155 |
Endoplasmic Reticulum | 25 | 23 | 18 | 14 | 34 | 50 | 81 | 49 | 40 | 39 | 39 | 69 | 6 | 12 | 15 | 6 | 3 | 7 | 53 | 20 | 22 | 12 | 15 | 26 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 |
Golgi | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 0 | 0 | 3 | 16 | 23 | 20 | 27 | 45 | 35 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 2 | 0 | 0 | 0 | 1 |
Nucleus | 0 | 1 | 3 | 1 | 3 | 1 | 2 | 2 | 1 | 1 | 1 | 3 | 0 | 1 | 0 | 2 | 1 | 6 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 4 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 0 | 0 | 0 | 0 | 2 | 1 | 0 | 9 | 4 | 4 | 4 | 2 | 0 | 0 | 1 | 4 | 3 | 0 | 0 | 3 | 1 | 1 | 1 | 2 |
Unique Cell Count | 314 | 297 | 175 | 238 | 275 | 361 | 425 | 497 | 289 | 368 | 378 | 426 | 225 | 276 | 291 | 303 | 250 | 311 | 318 | 311 | 186 | 62 | 140 | 239 |
Labelled Cell Count | 435 | 420 | 238 | 346 | 360 | 448 | 526 | 587 | 338 | 448 | 478 | 539 | 317 | 393 | 431 | 414 | 308 | 416 | 318 | 311 | 186 | 62 | 140 | 239 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 11.7 | 13.8 | 13.8 | 13.1 | 12.3 | 9.7 | 9.4 | 8.8 | 7.8 | 8.1 | 7.5 | 7.8 | 14.0 | 13.7 | 14.1 | 17.8 | 17.6 | 17.7 | 11.2 | 14.1 | 14.6 |
Std Deviation (1e-4) | 1.6 | 2.1 | 1.7 | 1.5 | 1.5 | 1.3 | 1.5 | 1.4 | 1.5 | 1.2 | 1.3 | 1.2 | 1.9 | 2.3 | 2.1 | 3.3 | 3.0 | 3.4 | 1.2 | 2.4 | 2.6 |
Intensity Change (Log2) | – | – | – | -0.07 | -0.17 | -0.51 | -0.56 | -0.65 | -0.82 | -0.78 | -0.88 | -0.83 | 0.02 | -0.01 | 0.03 | 0.37 | 0.35 | 0.35 | -0.3 | 0.03 | 0.08 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 3.0 | 3.1 | 0 | 3.7 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 3.0 | 1.6 | -2.3 | -4.8 | -7.3 | -6.5 | -5.5 | -4.7 | -3.5 | 0.4 | 0.9 | 2.0 | 1.4 | -1.9 | 0.6 |
Cytoplasm | 0 | -3.8 | -2.6 | -2.6 | -1.0 | -2.4 | -1.8 | -2.2 | -4.1 | 3.8 | 2.9 | 2.7 | 0.5 | 1.2 | 0.6 |
Endoplasmic Reticulum | -1.7 | 0.7 | 1.2 | 2.6 | -0.2 | 1.1 | 0.1 | 0 | 1.9 | -3.2 | -2.5 | -2.1 | -4.0 | -4.3 | -3.8 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 0 | 2.9 | 3.6 | 3.7 | 4.8 | 3.9 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 10.8381 | 11.7055 | 11.3665 | 10.6377 | 9.5598 | 10.9792 | 7.7789 | 9.7317 | 9.0945 | 8.6877 | 6.6481 | 8.8095 | 7.9991 | 10.7109 | 9.579 | 9.9698 | 8.0415 | 9.5299 |
Actin | 0.0048 | 0.0078 | 0.0113 | 0.0067 | 0.009 | 0.0029 | 0.0276 | 0.0138 | 0.0232 | 0.0201 | 0.0163 | 0.0139 | 0.0247 | 0.0047 | 0.0173 | 0.0099 | 0.0198 | 0.0092 |
Bud | 0.0027 | 0.0004 | 0.0056 | 0.001 | 0.0006 | 0.0005 | 0.0008 | 0.0004 | 0.0018 | 0.0013 | 0.0028 | 0.0006 | 0.005 | 0.0003 | 0.0078 | 0.0024 | 0.0009 | 0.0003 |
Bud Neck | 0.0021 | 0.0015 | 0.006 | 0.0064 | 0.0214 | 0.0112 | 0.0047 | 0.0018 | 0.0052 | 0.0037 | 0.0037 | 0.0116 | 0.0045 | 0.0027 | 0.0056 | 0.0069 | 0.0066 | 0.0247 |
Bud Periphery | 0.0052 | 0.0011 | 0.0091 | 0.0029 | 0.0021 | 0.002 | 0.0016 | 0.0007 | 0.0074 | 0.0048 | 0.0031 | 0.0021 | 0.0047 | 0.0006 | 0.0045 | 0.0134 | 0.0024 | 0.0008 |
Bud Site | 0.0022 | 0.0063 | 0.0099 | 0.0012 | 0.0028 | 0.0006 | 0.0102 | 0.0172 | 0.0081 | 0.0014 | 0.0029 | 0.0007 | 0.0154 | 0.0143 | 0.0268 | 0.0023 | 0.002 | 0.0004 |
Cell Periphery | 0.0475 | 0.0281 | 0.0224 | 0.0185 | 0.0227 | 0.0217 | 0.0425 | 0.022 | 0.0296 | 0.0121 | 0.0121 | 0.0171 | 0.0314 | 0.0166 | 0.0233 | 0.045 | 0.014 | 0.0094 |
Cytoplasm | 0.865 | 0.9027 | 0.9024 | 0.9389 | 0.848 | 0.9093 | 0.7405 | 0.8891 | 0.8372 | 0.8782 | 0.6824 | 0.8857 | 0.6958 | 0.9381 | 0.8598 | 0.8597 | 0.7598 | 0.8972 |
Cytoplasmic Foci | 0.0029 | 0.0028 | 0.0019 | 0.0021 | 0.0032 | 0.0058 | 0.0216 | 0.004 | 0.0064 | 0.0034 | 0.0138 | 0.01 | 0.0222 | 0.0017 | 0.0041 | 0.0059 | 0.0177 | 0.0039 |
Eisosomes | 0.0003 | 0.0003 | 0.0001 | 0.0001 | 0.0003 | 0.0001 | 0.0004 | 0.0001 | 0.0003 | 0 | 0.0001 | 0.0003 | 0.0006 | 0 | 0.0003 | 0.0002 | 0.0002 | 0 |
Endoplasmic Reticulum | 0.0462 | 0.03 | 0.0215 | 0.0146 | 0.0685 | 0.0343 | 0.0702 | 0.0402 | 0.0457 | 0.0076 | 0.1056 | 0.0295 | 0.0608 | 0.0154 | 0.0141 | 0.0149 | 0.0321 | 0.028 |
Endosome | 0.0019 | 0.0017 | 0.0017 | 0.0009 | 0.0033 | 0.0018 | 0.0279 | 0.0013 | 0.005 | 0.0398 | 0.0787 | 0.0075 | 0.0327 | 0.0006 | 0.0036 | 0.0006 | 0.0381 | 0.0047 |
Golgi | 0.0029 | 0.0028 | 0.0019 | 0.001 | 0.0044 | 0.0016 | 0.019 | 0.0023 | 0.0064 | 0.0036 | 0.0254 | 0.0078 | 0.0208 | 0.0009 | 0.004 | 0.001 | 0.0281 | 0.0059 |
Lipid Particles | 0.0049 | 0.0068 | 0.0005 | 0.0009 | 0.0007 | 0.0026 | 0.0102 | 0.0035 | 0.0094 | 0.0086 | 0.0056 | 0.0027 | 0.0187 | 0.0013 | 0.0057 | 0.0012 | 0.0088 | 0.0022 |
Mitochondria | 0.0059 | 0.0037 | 0.0002 | 0.0002 | 0.0088 | 0.0003 | 0.005 | 0.0001 | 0.0034 | 0.0015 | 0.0317 | 0.0021 | 0.0246 | 0 | 0.0112 | 0.0022 | 0.0562 | 0.0058 |
None | 0.0015 | 0.0008 | 0.0009 | 0.0005 | 0.0016 | 0.0009 | 0.0016 | 0.0005 | 0.0006 | 0.0009 | 0.0007 | 0.0006 | 0.0033 | 0.0003 | 0.0008 | 0.0302 | 0.001 | 0.0003 |
Nuclear Periphery | 0.0005 | 0.0005 | 0.0004 | 0.0007 | 0.0004 | 0.0003 | 0.0018 | 0.0005 | 0.001 | 0.0026 | 0.0017 | 0.0013 | 0.0118 | 0.0003 | 0.0006 | 0.0005 | 0.0023 | 0.0003 |
Nucleolus | 0.0001 | 0 | 0.0001 | 0 | 0 | 0.0002 | 0.0001 | 0 | 0.0001 | 0.0002 | 0.0002 | 0 | 0.0006 | 0 | 0.0001 | 0.0001 | 0.0001 | 0 |
Nucleus | 0.0004 | 0.0004 | 0.0007 | 0.0006 | 0.0004 | 0.001 | 0.0007 | 0.0004 | 0.0004 | 0.0007 | 0.0016 | 0.0004 | 0.002 | 0.0004 | 0.0008 | 0.0005 | 0.0008 | 0.0003 |
Peroxisomes | 0.0008 | 0.0003 | 0.0001 | 0.0001 | 0.0002 | 0.0003 | 0.0069 | 0.0001 | 0.0033 | 0.0003 | 0.0023 | 0.0043 | 0.0081 | 0 | 0.0034 | 0.0002 | 0.004 | 0.0051 |
Punctate Nuclear | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0009 | 0.0009 | 0.0001 | 0.0005 | 0.0003 | 0.0005 | 0.0003 | 0.0061 | 0.0001 | 0.0006 | 0.0002 | 0.0014 | 0.0001 |
Vacuole | 0.0023 | 0.0018 | 0.0029 | 0.0025 | 0.0013 | 0.0017 | 0.0053 | 0.0019 | 0.0049 | 0.0069 | 0.006 | 0.0012 | 0.0048 | 0.0016 | 0.0053 | 0.0028 | 0.0027 | 0.0011 |
Vacuole Periphery | 0.0001 | 0.0001 | 0.0001 | 0.0002 | 0.0002 | 0.0001 | 0.0005 | 0 | 0.0002 | 0.0018 | 0.0027 | 0.0002 | 0.0013 | 0 | 0.0003 | 0.0001 | 0.0007 | 0.0002 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 34.2977 | 39.712 | 30.7681 | 37.3576 | 34.3042 | 46.3578 | 32.0336 | 37.0638 | 40.8783 | 34.4542 |
Translational Efficiency | 1.2537 | 0.9858 | 1.4165 | 1.165 | 1.1125 | 0.9307 | 0.9643 | 0.91 | 0.8989 | 1.1091 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
1136 | 1070 | 1105 | 831 | 1897 | 1607 | 176 | 558 | 3033 | 2677 | 1281 | 1389 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 925.84 | 1087.40 | 1455.01 | 1234.30 | 1205.51 | 1151.22 | 1396.30 | 1224.67 | 1100.76 | 1125.71 | 1446.94 | 1230.43 |
Standard Deviation | 134.32 | 217.27 | 183.65 | 180.02 | 161.66 | 172.33 | 174.98 | 179.55 | 203.53 | 194.09 | 183.60 | 179.89 |
Intensity Change Log 2 | — | 0.232048 | 0.652194 | 0.414858 | — | -0.066480 | 0.211965 | 0.022749 | — | 0.070842 | 0.419857 | 0.206287 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.003460 | 0.005303 | 0.051551 | 0.055756 | 0.005199 | 0.007988 | 0.089088 | 0.068798 | 0.004548 | 0.006915 | 0.056708 | 0.060995 |
Bud Neck | 0.006506 | 0.005556 | 0.021381 | 0.020801 | 0.004076 | 0.008078 | 0.020920 | 0.021611 | 0.004986 | 0.007070 | 0.021317 | 0.021127 |
Bud Site | 0.092896 | 0.095196 | 0.149997* | 0.136698* | 0.049625 | 0.078121 | 0.173921* | 0.141795* | 0.065832 | 0.084946 | 0.153284* | 0.138746* |
Cell Periphery | 0.027918 | 0.013950 | 0.108209* | 0.099678 | 0.019484 | 0.008146 | 0.125109* | 0.123709* | 0.022643 | 0.010466 | 0.110531* | 0.109332* |
Cytoplasm | 0.699542* | 0.653942* | 0.387367* | 0.409697* | 0.785879* | 0.687620* | 0.184393* | 0.393154* | 0.753542* | 0.674158* | 0.359480* | 0.403051* |
Cytoplasmic Foci | 0.083502 | 0.110271 | 0.017271 | 0.059201 | 0.057512 | 0.102976 | 0.074226 | 0.048706 | 0.067247 | 0.105892 | 0.025096 | 0.054985 |
Eisosomes | 0.000981 | 0.000304 | 0.001722 | 0.005223 | 0.000593 | 0.000707 | 0.005632 | 0.006915 | 0.000738 | 0.000546 | 0.002259 | 0.005902 |
Endoplasmic Reticulum | 0.028789 | 0.020898 | 0.051656 | 0.009183 | 0.026750 | 0.011444 | 0.052159 | 0.008238 | 0.027514 | 0.015223 | 0.051725 | 0.008803 |
Endosome | 0.006982 | 0.016751 | 0.005529 | 0.014989 | 0.003383 | 0.013044 | 0.027905 | 0.014576 | 0.004731 | 0.014526 | 0.008604 | 0.014823 |
Golgi | 0.014212 | 0.016174 | 0.007451 | 0.006417 | 0.009182 | 0.014465 | 0.008542 | 0.007920 | 0.011066 | 0.015148 | 0.007601 | 0.007021 |
Lipid Particles | 0.006207 | 0.015160 | 0.133424* | 0.047624 | 0.011587 | 0.008689 | 0.060258 | 0.043220 | 0.009572 | 0.011276 | 0.123372* | 0.045855 |
Mitochondria | 0.004118 | 0.000473 | 0.000187 | 0.001385 | 0.000906 | 0.002635 | 0.000027 | 0.001774 | 0.002109 | 0.001771 | 0.000165 | 0.001542 |
Mitotic Spindle | 0.000153 | 0.000328 | 0.000645 | 0.003647 | 0.000249 | 0.002028 | 0.000370 | 0.002352 | 0.000213 | 0.001349 | 0.000607 | 0.003127 |
None | 0.000695 | 0.000563 | 0.000263 | 0.000305 | 0.000660 | 0.000261 | 0.000027 | 0.000092 | 0.000673 | 0.000382 | 0.000230 | 0.000219 |
Nuclear Periphery | 0.000075 | 0.000084 | 0.000152 | 0.000209 | 0.000039 | 0.000027 | 0.000122 | 0.000167 | 0.000052 | 0.000050 | 0.000148 | 0.000192 |
Nuclear Periphery Foci | 0.000681 | 0.002467 | 0.006184 | 0.002959 | 0.000815 | 0.000506 | 0.007600 | 0.002344 | 0.000765 | 0.001290 | 0.006378 | 0.002712 |
Nucleolus | 0.000376 | 0.000264 | 0.000038 | 0.000070 | 0.000081 | 0.000187 | 0.000020 | 0.000040 | 0.000191 | 0.000218 | 0.000036 | 0.000058 |
Nucleus | 0.003188 | 0.002428 | 0.006421 | 0.004691 | 0.003488 | 0.004716 | 0.002747 | 0.004288 | 0.003376 | 0.003802 | 0.005916 | 0.004529 |
Peroxisomes | 0.006216 | 0.002745 | 0.002773 | 0.006169 | 0.003501 | 0.003900 | 0.002955 | 0.007480 | 0.004518 | 0.003439 | 0.002798 | 0.006695 |
Vacuole | 0.008945 | 0.031598 | 0.046989 | 0.114086 | 0.015556 | 0.041743 | 0.163559 | 0.100393 | 0.013080 | 0.037688 | 0.063005 | 0.108585 |
Vacuole Periphery | 0.004556 | 0.005544 | 0.000791 | 0.001212 | 0.001437 | 0.002719 | 0.000419 | 0.002429 | 0.002605 | 0.003848 | 0.000740 | 0.001701 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -3.22 | -18.68 | -13.67 | -13.08 | -0.62 | -3.76 | -7.75 | -13.06 | -12.43 | 1.67 | -4.70 | -19.36 | -18.70 | -17.80 | -0.86 |
Bud Neck | 1.67 | -13.91 | -9.10 | -9.80 | 0.55 | -6.34 | -8.23 | -9.95 | -7.31 | -0.12 | -4.71 | -17.78 | -13.87 | -11.63 | 0.42 |
Bud Site | -0.43 | -9.20 | -6.11 | -5.72 | 1.98 | -7.77 | -8.21 | -13.02 | -8.60 | 2.05 | -6.15 | -17.07 | -14.89 | -10.56 | 2.52 |
Cell Periphery | 5.56 | -16.42 | -10.35 | -12.87 | 1.40 | 8.61 | -7.25 | -12.22 | -13.70 | 0.24 | 9.65 | -19.90 | -16.33 | -18.99 | 0.52 |
Cytoplasm | 4.24 | 27.61 | 24.91 | 19.83 | -1.43 | 12.30 | 45.05 | 32.13 | 22.88 | -12.32 | 12.28 | 43.41 | 42.38 | 31.01 | -3.72 |
Cytoplasmic Foci | -5.01 | 20.92 | 4.56 | 8.45 | -11.41 | -10.98 | -2.09 | 1.65 | 9.46 | 2.77 | -11.79 | 18.31 | 3.15 | 12.39 | -9.70 |
Eisosomes | 4.37 | -4.00 | -7.74 | -9.40 | -6.52 | -1.55 | -7.06 | -8.75 | -8.58 | -1.15 | 2.66 | -10.15 | -11.95 | -12.50 | -8.04 |
Endoplasmic Reticulum | 2.17 | -4.62 | 6.27 | 4.45 | 10.15 | 5.89 | -2.59 | 7.10 | 1.75 | 4.61 | 5.76 | -5.90 | 9.34 | 4.19 | 11.36 |
Endosome | -6.60 | 1.74 | -5.46 | 0.75 | -7.16 | -10.10 | -4.94 | -5.70 | -0.62 | 2.53 | -11.96 | -4.65 | -8.88 | -0.33 | -4.72 |
Golgi | -1.66 | 8.07 | 5.50 | 6.59 | 0.95 | -4.95 | 0.45 | 0.99 | 3.83 | 0.38 | -5.14 | 6.82 | 4.19 | 7.14 | 0.77 |
Lipid Particles | -6.40 | -24.70 | -10.65 | -7.97 | 13.30 | 3.01 | -6.98 | -7.72 | -8.45 | 2.10 | -2.10 | -24.71 | -12.67 | -11.96 | 14.34 |
Mitochondria | 5.17 | 5.59 | 2.68 | -1.12 | -1.49 | -2.91 | 5.65 | -0.94 | 0.89 | -1.99 | 0.79 | 6.88 | 0.98 | 0.41 | -2.31 |
Mitotic Spindle | -3.24 | -13.83 | -2.84 | -2.71 | -2.47 | -2.34 | -1.04 | -1.74 | -0.13 | -1.64 | -2.47 | -6.73 | -3.30 | -1.79 | -2.88 |
None | 0.63 | 2.28 | 1.61 | 1.32 | -0.20 | 3.43 | 5.59 | 4.93 | 4.94 | -3.72 | 2.64 | 4.29 | 3.26 | 1.49 | 0.15 |
Nuclear Periphery | -0.16 | -1.89 | -1.28 | -1.23 | -0.58 | 1.12 | -5.77 | -1.89 | -2.06 | -0.63 | 0.09 | -5.63 | -2.13 | -2.17 | -0.67 |
Nuclear Periphery Foci | -4.97 | -19.21 | -6.84 | -1.36 | 7.30 | 3.55 | -8.53 | -6.36 | -7.54 | 6.22 | -3.45 | -21.60 | -8.94 | -5.82 | 10.55 |
Nucleolus | 0.58 | 1.84 | 1.66 | 1.22 | -3.54 | -1.06 | 2.42 | 1.62 | 1.51 | -3.37 | -0.10 | 2.17 | 1.85 | 1.84 | -3.69 |
Nucleus | 0.72 | -3.07 | -1.56 | -4.28 | 2.96 | -1.56 | 1.38 | -1.44 | 0.20 | -3.13 | -0.64 | -4.14 | -2.37 | -1.88 | 2.62 |
Peroxisomes | 6.29 | 6.34 | 0.24 | -7.25 | -7.34 | -1.26 | 1.65 | -2.97 | -2.67 | -3.39 | 3.84 | 6.23 | -3.31 | -5.37 | -6.53 |
Vacuole | -11.47 | -21.24 | -20.29 | -15.44 | -12.70 | -12.17 | -10.78 | -13.61 | -9.35 | 3.69 | -16.13 | -18.71 | -23.90 | -17.36 | -10.34 |
Vacuole Periphery | -1.26 | 7.30 | 5.64 | 6.52 | -1.31 | -4.59 | 6.45 | -0.81 | 0.32 | -1.73 | -3.53 | 8.45 | 1.79 | 3.93 | -1.90 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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