Standard name | |
---|---|
Human Ortholog | |
Description | Protein that localizes to sites of polarized growth; required for selection and/or maintenance of polarized growth sites, may modulate signaling by the GTPases Cdc42p and Rho1p; contains LIM domains and has similarity to metazoan paxillin; relocalizes from bud neck to cytoplasm upon DNA replication stress |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 65 | 38 | 60 | 29 | 36 | 35 | 27 | 15 | 19 | 17 | 6 | 9 | 10 | 6 | 1 | 16 | 16 | 3 | 32 | 12 | 15 | 3 | 20 | 13 |
Bud | 74 | 27 | 39 | 13 | 25 | 32 | 46 | 32 | 27 | 29 | 24 | 31 | 7 | 3 | 2 | 5 | 7 | 3 | 107 | 66 | 70 | 6 | 10 | 18 |
Bud Neck | 7 | 9 | 13 | 6 | 12 | 13 | 16 | 15 | 1 | 5 | 3 | 11 | 0 | 0 | 0 | 0 | 2 | 0 | 64 | 46 | 62 | 8 | 8 | 9 |
Bud Site | 38 | 21 | 44 | 26 | 12 | 28 | 14 | 6 | 10 | 7 | 3 | 4 | 11 | 2 | 1 | 9 | 7 | 1 | – | – | – | – | – | – |
Cell Periphery | 9 | 10 | 9 | 16 | 3 | 2 | 6 | 7 | 9 | 2 | 5 | 7 | 1 | 8 | 1 | 13 | 13 | 3 | 2 | 1 | 2 | 0 | 2 | 1 |
Cytoplasm | 153 | 174 | 212 | 222 | 272 | 317 | 430 | 431 | 216 | 212 | 219 | 288 | 87 | 190 | 228 | 53 | 63 | 9 | 116 | 142 | 169 | 23 | 80 | 94 |
Endoplasmic Reticulum | 2 | 8 | 6 | 2 | 3 | 3 | 2 | 0 | 2 | 5 | 2 | 4 | 1 | 0 | 0 | 2 | 3 | 0 | 2 | 1 | 1 | 1 | 8 | 5 |
Endosome | 22 | 7 | 1 | 1 | 1 | 3 | 3 | 4 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 0 | 1 | 5 | 4 | 2 | 1 | 1 | 2 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 2 | 0 | 0 | 0 |
Mitochondria | 14 | 11 | 2 | 6 | 7 | 62 | 83 | 66 | 130 | 114 | 121 | 104 | 3 | 1 | 4 | 5 | 3 | 4 | 3 | 3 | 17 | 2 | 5 | 9 |
Nucleus | 6 | 0 | 3 | 2 | 0 | 3 | 3 | 9 | 3 | 2 | 2 | 4 | 0 | 2 | 0 | 1 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 1 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 3 | 3 | 1 | 1 | 0 | 4 | 4 | 7 | 1 | 1 | 1 | 2 | 2 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 19 | 12 | 2 | 4 | 0 | 15 | 4 | 1 | 3 | 2 | 0 | 1 | 7 | 3 | 2 | 0 | 2 | 1 | 0 | 0 | 1 | 1 | 0 | 0 |
SpindlePole | 35 | 5 | 45 | 30 | 18 | 47 | 38 | 34 | 12 | 9 | 7 | 17 | 2 | 1 | 0 | 7 | 7 | 1 | 8 | 5 | 8 | 0 | 0 | 1 |
Vac/Vac Membrane | 4 | 3 | 6 | 4 | 3 | 4 | 3 | 23 | 2 | 8 | 5 | 2 | 0 | 0 | 0 | 3 | 10 | 0 | 4 | 8 | 7 | 1 | 4 | 3 |
Unique Cell Count | 320 | 264 | 332 | 292 | 340 | 448 | 568 | 560 | 336 | 332 | 327 | 418 | 112 | 199 | 233 | 87 | 101 | 18 | 353 | 297 | 368 | 54 | 143 | 160 |
Labelled Cell Count | 451 | 328 | 443 | 362 | 392 | 568 | 679 | 652 | 436 | 415 | 398 | 484 | 131 | 216 | 240 | 117 | 138 | 26 | 353 | 297 | 368 | 54 | 143 | 160 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 6.3 | 6.4 | 6.3 | 5.8 | 6.3 | 4.9 | 4.9 | 5.1 | 4.2 | 4.0 | 4.0 | 4.4 | 6.0 | 6.4 | 6.4 | 10.0 | 10.5 | 9.9 | 5.8 | 5.8 | 6.3 |
Std Deviation (1e-4) | 0.9 | 1.1 | 0.7 | 1.0 | 1.3 | 1.0 | 0.9 | 1.0 | 0.8 | 0.6 | 0.7 | 0.8 | 0.6 | 0.9 | 1.1 | 1.7 | 2.0 | 1.7 | 1.6 | 1.4 | 1.5 |
Intensity Change (Log2) | – | – | – | -0.12 | -0.01 | -0.36 | -0.38 | -0.3 | -0.58 | -0.65 | -0.67 | -0.51 | -0.08 | 0.02 | 0.03 | 0.67 | 0.74 | 0.65 | -0.12 | -0.12 | 0.0 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | -2.9 | -2.8 | -4.3 | 0 | -8.0 | 0 | 0 | 0 | 0 | -2.3 | -5.1 | -6.7 | 0.1 | -0.5 | 0 |
Bud | -3.3 | -1.9 | -2.2 | 0 | -3.2 | 0 | 0 | 0 | 0 | -1.7 | -4.2 | -4.9 | -1.6 | -1.4 | 0 |
Bud Neck | -1.3 | -0.3 | -0.8 | 0 | -1.0 | 0 | 0 | 0 | 0 | 0 | 0 | -3.1 | 0 | 0 | 0 |
Bud Site | -1.7 | -4.6 | -3.3 | 0 | -7.6 | 0 | 0 | 0 | 0 | -1.0 | -4.9 | -5.5 | -0.7 | -1.7 | 0 |
Cell Periphery | 1.8 | -1.8 | 0 | 0 | -1.6 | 0 | 0 | 0 | 0 | 0 | 0.8 | 0 | 0 | 4.1 | 0 |
Cytoplasm | 3.3 | 4.7 | 2.0 | 3.8 | 4.2 | 0.1 | 0 | 0.8 | 1.5 | 2.7 | 8.2 | 9.6 | -0.5 | -0.3 | -1.2 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 6.7 | 0 | 6.1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 2.6 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | -1.3 | -3.7 | -1.3 | 0 | -3.8 | 0 | 0 | 0 | 0 | -3.5 | -5.2 | -5.9 | -1.4 | -1.8 | 0 |
Vacuole | 0 | 0 | 0 | 0 | 1.9 | 0 | 0.5 | -0.3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 1.5217 | 1.5338 | 1.7657 | 1.857 | 1.7957 | 1.5617 | 1.5609 | 1.8027 | 2.0236 | 1.8129 | 1.6663 | 1.6905 | 1.3612 | 1.657 | 1.4639 | 2.2408 | 1.3683 | 1.5724 |
Actin | 0.039 | 0.0268 | 0.0202 | 0.0123 | 0.004 | 0.0266 | 0.0413 | 0.0106 | 0.0288 | 0.0313 | 0.0007 | 0.0225 | 0.1187 | 0.0065 | 0.0378 | 0.002 | 0.0152 | 0.0107 |
Bud | 0.0551 | 0.0531 | 0.2451 | 0.0274 | 0.0111 | 0.0072 | 0.0485 | 0.0463 | 0.168 | 0.0702 | 0.0015 | 0.0079 | 0.0539 | 0.0622 | 0.2089 | 0.0315 | 0.0156 | 0.0055 |
Bud Neck | 0.0803 | 0.0062 | 0.0057 | 0.0455 | 0.0415 | 0.5547 | 0.0851 | 0.0048 | 0.0099 | 0.0036 | 0.0526 | 0.5746 | 0.0571 | 0.0058 | 0.0125 | 0.0135 | 0.0242 | 0.4855 |
Bud Periphery | 0.0908 | 0.0367 | 0.3008 | 0.0812 | 0.011 | 0.01 | 0.0403 | 0.0358 | 0.2133 | 0.0651 | 0.0017 | 0.0106 | 0.0741 | 0.0447 | 0.2645 | 0.0713 | 0.0346 | 0.0054 |
Bud Site | 0.1936 | 0.3048 | 0.0812 | 0.065 | 0.0284 | 0.0071 | 0.2204 | 0.389 | 0.0927 | 0.0502 | 0.0023 | 0.0233 | 0.1439 | 0.2542 | 0.0343 | 0.0354 | 0.0616 | 0.0131 |
Cell Periphery | 0.003 | 0.002 | 0.0019 | 0.0036 | 0.0004 | 0.0009 | 0.0058 | 0.0026 | 0.0027 | 0.0016 | 0.0004 | 0.0008 | 0.0036 | 0.0023 | 0.0013 | 0.0009 | 0.0022 | 0.0005 |
Cytoplasm | 0.2716 | 0.4446 | 0.243 | 0.5295 | 0.7275 | 0.2466 | 0.297 | 0.39 | 0.3497 | 0.5567 | 0.77 | 0.2889 | 0.3822 | 0.4432 | 0.3252 | 0.69 | 0.5504 | 0.3317 |
Cytoplasmic Foci | 0.0777 | 0.03 | 0.0208 | 0.0321 | 0.0674 | 0.0594 | 0.0819 | 0.038 | 0.0232 | 0.0388 | 0.0387 | 0.0304 | 0.0335 | 0.0208 | 0.022 | 0.0056 | 0.0301 | 0.0829 |
Eisosomes | 0.0004 | 0.0002 | 0.0002 | 0.0003 | 0 | 0.0001 | 0.0003 | 0.0001 | 0.0002 | 0.0004 | 0 | 0 | 0.0008 | 0.0001 | 0.0003 | 0.0001 | 0.0004 | 0 |
Endoplasmic Reticulum | 0.0045 | 0.0023 | 0.0024 | 0.0045 | 0.0044 | 0.0019 | 0.0099 | 0.0024 | 0.0042 | 0.0035 | 0.0025 | 0.0011 | 0.0051 | 0.0019 | 0.0036 | 0.0031 | 0.007 | 0.0006 |
Endosome | 0.0407 | 0.0071 | 0.0112 | 0.0382 | 0.0409 | 0.013 | 0.0295 | 0.0085 | 0.026 | 0.028 | 0.0586 | 0.0077 | 0.0191 | 0.0025 | 0.0232 | 0.0041 | 0.0744 | 0.0076 |
Golgi | 0.0128 | 0.0035 | 0.003 | 0.0104 | 0.0041 | 0.011 | 0.011 | 0.002 | 0.0117 | 0.0076 | 0.0177 | 0.0037 | 0.0087 | 0.0008 | 0.0063 | 0.0003 | 0.0085 | 0.0019 |
Lipid Particles | 0.0151 | 0.001 | 0.004 | 0.0026 | 0.0198 | 0.0023 | 0.0144 | 0.0003 | 0.0049 | 0.0051 | 0.0108 | 0.0005 | 0.0054 | 0.0003 | 0.0026 | 0.0002 | 0.0109 | 0.0021 |
Mitochondria | 0.0242 | 0.006 | 0.008 | 0.0054 | 0.0059 | 0.0115 | 0.0291 | 0.0004 | 0.0038 | 0.03 | 0.0019 | 0.0055 | 0.004 | 0.0005 | 0.0125 | 0.0003 | 0.009 | 0.0005 |
None | 0.0345 | 0.056 | 0.0348 | 0.099 | 0.0064 | 0.033 | 0.0309 | 0.0557 | 0.0465 | 0.0749 | 0.0065 | 0.0136 | 0.0619 | 0.1379 | 0.0319 | 0.1359 | 0.0545 | 0.0394 |
Nuclear Periphery | 0.0114 | 0.0007 | 0.0012 | 0.0036 | 0.0014 | 0.0006 | 0.0184 | 0.0006 | 0.0013 | 0.0014 | 0.001 | 0.0004 | 0.0042 | 0.0006 | 0.0022 | 0.0006 | 0.0092 | 0.0001 |
Nucleolus | 0.0038 | 0.0005 | 0.0004 | 0.0007 | 0.0012 | 0.0003 | 0.0006 | 0.0004 | 0.0004 | 0.0007 | 0.0004 | 0.0002 | 0.0019 | 0.0011 | 0.0003 | 0.0001 | 0.0124 | 0.0002 |
Nucleus | 0.0095 | 0.002 | 0.0013 | 0.021 | 0.0016 | 0.0007 | 0.0061 | 0.0018 | 0.0016 | 0.0023 | 0.0012 | 0.0011 | 0.0022 | 0.0067 | 0.0009 | 0.0008 | 0.021 | 0.0004 |
Peroxisomes | 0.0165 | 0.007 | 0.0075 | 0.0017 | 0.0094 | 0.008 | 0.0131 | 0.0034 | 0.0044 | 0.0105 | 0.0078 | 0.0037 | 0.0063 | 0.001 | 0.0032 | 0.0001 | 0.0094 | 0.0093 |
Punctate Nuclear | 0.0075 | 0.001 | 0.002 | 0.0023 | 0.0063 | 0.0028 | 0.003 | 0.0025 | 0.0016 | 0.0019 | 0.0074 | 0.0012 | 0.0086 | 0.0016 | 0.0012 | 0.0004 | 0.027 | 0.0013 |
Vacuole | 0.0058 | 0.0074 | 0.0044 | 0.0112 | 0.0059 | 0.0016 | 0.0115 | 0.0045 | 0.004 | 0.0144 | 0.013 | 0.002 | 0.004 | 0.005 | 0.0042 | 0.0035 | 0.0174 | 0.001 |
Vacuole Periphery | 0.002 | 0.0009 | 0.0008 | 0.0025 | 0.0014 | 0.0004 | 0.002 | 0.0003 | 0.001 | 0.0017 | 0.0032 | 0.0004 | 0.001 | 0.0003 | 0.001 | 0.0005 | 0.0049 | 0.0001 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 22.6299 | 8.836 | 10.0076 | 18.6484 | 24.6192 | 15.0783 | 15.9873 | 11.9011 | 11.8447 | 23.1497 |
Translational Efficiency | 1.5384 | 2.4336 | 1.4636 | 1.0212 | 1.2668 | 2.2851 | 1.007 | 1.4047 | 1.5123 | 1.3192 |
Dataset | Hit |
---|---|
Protein Concentration | ✔ |
Protein Localization | ✘ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
1490 | 518 | 788 | 1229 | 1749 | 447 | 769 | 107 | 3239 | 965 | 1557 | 1336 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 777.32 | 823.52 | 1132.62 | 1006.19 | 822.03 | 776.74 | 1076.40 | 1001.90 | 801.46 | 801.85 | 1104.85 | 1005.85 |
Standard Deviation | 90.89 | 107.51 | 139.99 | 123.07 | 94.23 | 108.19 | 110.06 | 127.44 | 95.35 | 110.32 | 129.20 | 123.43 |
Intensity Change Log 2 | — | 0.083295 | 0.543083 | 0.372322 | — | -0.081759 | 0.388951 | 0.285476 | — | 0.000821 | 0.465921 | 0.328338 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000660 | 0.003133 | 0.002896 | 0.003252 | 0.000699 | 0.001076 | 0.001721 | 0.003972 | 0.000681 | 0.002180 | 0.002316 | 0.003309 |
Bud Neck | 0.094377 | 0.072677 | 0.057336 | 0.052463 | 0.099988 | 0.093563 | 0.092653 | 0.073993 | 0.097407 | 0.082352 | 0.074779 | 0.054188 |
Bud Site | 0.326265* | 0.327871* | 0.334507* | 0.404417* | 0.308217* | 0.306969* | 0.464179* | 0.535956* | 0.316519* | 0.318189* | 0.398552* | 0.414952* |
Cell Periphery | 0.001026 | 0.000607 | 0.000439 | 0.000389 | 0.000666 | 0.000424 | 0.000518 | 0.000343 | 0.000832 | 0.000522 | 0.000478 | 0.000386 |
Cytoplasm | 0.406949* | 0.342930* | 0.361102* | 0.413381* | 0.397617* | 0.316508* | 0.240107* | 0.225311* | 0.401910* | 0.330691* | 0.301343* | 0.398318* |
Cytoplasmic Foci | 0.102882 | 0.128743 | 0.041918 | 0.019606 | 0.108996 | 0.146179 | 0.027319 | 0.041056 | 0.106184 | 0.136819 | 0.034708 | 0.021324 |
Eisosomes | 0.000215 | 0.000240 | 0.000085 | 0.000065 | 0.000174 | 0.000212 | 0.000111 | 0.000117 | 0.000193 | 0.000227 | 0.000098 | 0.000069 |
Endoplasmic Reticulum | 0.001347 | 0.000433 | 0.001436 | 0.000471 | 0.000830 | 0.000385 | 0.001170 | 0.000492 | 0.001068 | 0.000410 | 0.001305 | 0.000472 |
Endosome | 0.010370 | 0.029247 | 0.007095 | 0.003230 | 0.012114 | 0.037775 | 0.004059 | 0.007096 | 0.011312 | 0.033198 | 0.005595 | 0.003540 |
Golgi | 0.006408 | 0.007308 | 0.000439 | 0.005477 | 0.004760 | 0.011197 | 0.000483 | 0.003176 | 0.005518 | 0.009110 | 0.000461 | 0.005292 |
Lipid Particles | 0.001547 | 0.002165 | 0.001636 | 0.000607 | 0.001773 | 0.002750 | 0.000738 | 0.000349 | 0.001669 | 0.002436 | 0.001193 | 0.000586 |
Mitochondria | 0.001458 | 0.000976 | 0.000286 | 0.001537 | 0.002360 | 0.005787 | 0.000105 | 0.002386 | 0.001945 | 0.003205 | 0.000196 | 0.001605 |
Mitotic Spindle | 0.000643 | 0.002130 | 0.004713 | 0.009793 | 0.001760 | 0.001854 | 0.003439 | 0.004624 | 0.001246 | 0.002002 | 0.004084 | 0.009379 |
None | 0.003452 | 0.000508 | 0.000730 | 0.000533 | 0.001209 | 0.001322 | 0.000224 | 0.000226 | 0.002241 | 0.000885 | 0.000480 | 0.000509 |
Nuclear Periphery | 0.000037 | 0.000084 | 0.000243 | 0.000107 | 0.000036 | 0.000037 | 0.000149 | 0.000096 | 0.000036 | 0.000062 | 0.000197 | 0.000106 |
Nuclear Periphery Foci | 0.000603 | 0.000456 | 0.003422 | 0.001021 | 0.000497 | 0.000278 | 0.002013 | 0.000870 | 0.000546 | 0.000373 | 0.002726 | 0.001009 |
Nucleolus | 0.000488 | 0.000553 | 0.000190 | 0.000174 | 0.000330 | 0.000310 | 0.000089 | 0.000106 | 0.000403 | 0.000441 | 0.000140 | 0.000168 |
Nucleus | 0.009923 | 0.007302 | 0.029380 | 0.008052 | 0.010444 | 0.005636 | 0.017918 | 0.004758 | 0.010204 | 0.006531 | 0.023719 | 0.007788 |
Peroxisomes | 0.002727 | 0.013449 | 0.002632 | 0.002258 | 0.002867 | 0.012798 | 0.003178 | 0.002100 | 0.002803 | 0.013147 | 0.002902 | 0.002245 |
Vacuole | 0.027061 | 0.057940 | 0.149204 | 0.070300 | 0.043362 | 0.051718 | 0.139530 | 0.088724 | 0.035863 | 0.055058 | 0.144426 | 0.071776 |
Vacuole Periphery | 0.001559 | 0.001248 | 0.000311 | 0.002867 | 0.001302 | 0.003223 | 0.000298 | 0.004251 | 0.001420 | 0.002163 | 0.000305 | 0.002978 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -2.46 | -2.17 | -5.14 | 0.39 | -0.15 | -1.82 | -3.94 | -1.59 | -1.46 | -1.30 | -2.74 | -3.00 | -5.20 | -1.08 | -1.40 |
Bud Neck | 2.78 | 7.89 | 10.75 | 5.31 | 2.11 | 0.66 | 4.80 | 3.42 | 2.60 | 0.72 | 2.46 | 8.82 | 13.93 | 7.76 | 4.18 |
Bud Site | 0.16 | 4.16 | 4.43 | 3.02 | -0.36 | 0.07 | -1.87 | -2.68 | -2.55 | -1.85 | 0.06 | 1.52 | 3.26 | 2.44 | 1.50 |
Cell Periphery | 2.07 | 5.12 | 6.05 | 1.84 | 2.72 | 3.24 | 3.45 | 3.82 | 1.01 | 1.41 | 2.79 | 6.21 | 7.85 | 2.32 | 3.47 |
Cytoplasm | 3.99 | 1.80 | -3.43 | -6.56 | -4.69 | 5.06 | 9.23 | 4.24 | 1.43 | 0.32 | 6.29 | 7.31 | -3.19 | -7.96 | -8.84 |
Cytoplasmic Foci | -3.02 | 13.31 | 18.30 | 13.12 | 5.81 | -3.82 | 18.61 | 8.06 | 8.91 | -1.47 | -4.76 | 22.45 | 25.54 | 18.07 | 4.27 |
Eisosomes | -0.41 | 6.90 | 8.05 | 3.18 | 2.26 | -1.09 | 4.27 | 0.73 | 1.29 | -0.65 | -0.95 | 8.02 | 9.69 | 4.73 | 3.27 |
Endoplasmic Reticulum | 2.53 | -0.76 | 2.63 | 0.24 | 6.92 | 2.71 | -2.01 | 1.39 | -0.58 | 2.98 | 3.49 | -1.91 | 3.32 | -0.07 | 7.02 |
Endosome | -5.45 | 3.57 | 8.63 | 7.94 | 6.60 | -6.36 | 8.62 | 2.37 | 6.78 | -0.61 | -8.29 | 8.57 | 12.11 | 11.73 | 5.02 |
Golgi | -0.83 | 13.73 | 2.54 | 2.68 | -2.57 | -4.14 | 11.61 | 1.50 | 4.05 | -1.21 | -3.92 | 17.90 | 2.09 | 4.29 | -2.71 |
Lipid Particles | -0.63 | -1.03 | 3.57 | 1.50 | 4.07 | -1.30 | 4.02 | 6.42 | 3.34 | 4.13 | -1.19 | 1.94 | 4.70 | 2.78 | 3.41 |
Mitochondria | 1.88 | 5.08 | 1.33 | -0.03 | -2.27 | -1.81 | 3.17 | 0.38 | 1.75 | -1.08 | -1.38 | 4.40 | 1.82 | 2.47 | -2.85 |
Mitotic Spindle | -1.69 | -3.24 | -4.13 | -2.96 | -0.77 | -0.11 | -1.89 | -1.37 | -1.34 | -0.30 | -1.19 | -3.65 | -3.89 | -3.33 | -1.33 |
None | 3.96 | 3.81 | 3.96 | -0.16 | 0.68 | -0.20 | 3.12 | 2.57 | 1.48 | -0.21 | 2.78 | 4.70 | 4.43 | 1.08 | -0.41 |
Nuclear Periphery | -0.97 | -10.71 | -6.04 | -1.89 | 4.52 | -0.05 | -9.65 | -2.04 | -2.01 | 2.66 | -0.97 | -14.45 | -6.70 | -3.54 | 4.50 |
Nuclear Periphery Foci | 0.73 | -9.40 | -1.10 | -1.55 | 7.05 | 2.63 | -7.98 | -2.23 | -3.16 | 4.48 | 1.60 | -12.29 | -1.54 | -2.14 | 6.91 |
Nucleolus | -0.63 | 2.89 | 2.82 | 1.20 | 0.37 | 0.25 | 3.23 | 2.43 | 2.24 | -0.13 | -0.64 | 4.17 | 3.17 | 1.40 | -0.35 |
Nucleus | 0.80 | -11.74 | -5.35 | -3.96 | 8.72 | 3.98 | -6.46 | 1.15 | -1.45 | 6.17 | 2.47 | -12.89 | -5.87 | -6.29 | 8.55 |
Peroxisomes | -5.68 | 1.24 | 2.36 | 6.09 | 0.67 | -4.57 | 0.72 | -0.12 | 3.71 | -0.40 | -7.24 | 1.30 | 2.41 | 7.74 | 0.72 |
Vacuole | -6.85 | -18.84 | -21.07 | -13.82 | 2.01 | -1.82 | -18.80 | -5.43 | -5.05 | 2.11 | -6.07 | -26.38 | -20.66 | -16.23 | 4.37 |
Vacuole Periphery | 1.32 | 6.48 | -0.29 | -0.79 | -2.29 | -1.93 | 6.01 | -0.80 | 0.08 | -1.30 | -1.54 | 8.99 | -0.71 | 0.39 | -2.59 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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