Standard name
Human Ortholog
Description Putative transporter of the MRP subfamily; adjacent ORFs YKR103W and YKR104W are merged in different strain backgrounds; MRP stands for multidrug resistance-associated protein
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.97 0.97 0.98 0.98 0.95 0.87 – 0.77 0.71 0.63 0.57 0.57 0.97 0.99 0.99 0.97 0.98 0.97 0.93 0.94 0.91 0.91 0.93 0.88
Endoplasmic Reticulum 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0.07 0.22 – 0.28 0.45 0.51 0.59 0.56 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0.05 – 0.08 0.07 0.09 0.06 0.05 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 1 7 1 1 3
Bud 0 1 0 0 2 5 – 8 14 10 25 13 0 0 1 0 0 0 1 1 9 3 3 8
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 1 0 0 0 0 0 0 2 0 0 0
Bud Site 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 6 0 6 3 2 3 – 6 5 11 8 9 7 2 4 0 2 1 0 0 0 0 0 0
Cytoplasm 347 227 599 363 539 564 – 677 421 484 421 498 216 272 284 140 149 73 337 219 542 342 376 352
Endoplasmic Reticulum 3 1 2 1 0 1 – 0 0 2 1 9 5 1 1 7 1 2 0 0 5 3 2 3
Endosome 2 1 5 0 4 11 – 16 5 7 4 8 0 0 0 0 0 0 7 2 5 1 2 2
Golgi 1 0 1 0 1 0 – 0 0 0 0 0 0 0 0 0 0 1 4 1 1 0 1 2
Mitochondria 2 3 6 15 40 145 – 247 271 389 434 489 0 0 0 5 5 1 1 1 5 6 7 8
Nucleus 0 2 0 2 2 2 – 10 3 7 9 7 0 0 3 0 0 1 0 0 0 0 0 0
Nuclear Periphery 0 1 1 0 0 0 – 3 1 7 6 5 0 0 0 0 0 0 0 0 0 1 0 2
Nucleolus 0 0 0 0 0 0 – 1 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 1 0 0 0 – 0 1 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0
SpindlePole 0 0 1 0 0 1 – 2 1 5 3 1 1 0 0 1 0 0 3 1 5 1 2 7
Vac/Vac Membrane 5 0 8 3 15 31 – 66 40 66 43 46 2 0 0 2 0 1 3 0 1 4 2 4
Unique Cell Count 358 233 614 369 566 647 877 596 765 739 869 223 274 286 144 152 75 364 234 595 375 406 401
Labelled Cell Count 366 236 630 387 605 763 1036 762 988 954 1086 231 276 293 155 157 80 364 234 595 375 406 401


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.9 5.9 5.4 5.3 4.9 4.3 – 4.4 4.1 3.8 3.8 3.9 6.2 6.3 6.1 5.2 6.0 7.3 6.4 6.8 7.0
Std Deviation (1e-4) 0.8 1.3 1.5 1.8 1.6 1.3 – 1.4 1.0 0.8 0.9 0.9 0.8 0.8 0.7 1.1 1.2 1.5 1.2 1.5 1.5
Intensity Change (Log2) – – – -0.02 -0.13 -0.32 – -0.27 -0.4 -0.51 -0.48 -0.48 0.21 0.22 0.19 -0.05 0.15 0.45 0.25 0.34 0.38


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.9 -2.2 -6.9 – -11.0 -12.9 -15.4 -17.3 -17.4 -0.6 1.7 1.8 -0.2 0.3 -0.1
Endoplasmic Reticulum 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 3.2 5.4 0 – 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 1.7 3.6 – 5.4 4.8 6.0 4.3 4.0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 0.844 1.2597 1.0515 0.8109 0.1912 1.1401 2.584 3.249 3.0825 2.287 2.7681 3.2894 1.9386 3.1847 2.5465 3.3388 2.3053 2.9712
Actin 0.021 0.0003 0.0084 0.0171 0.0003 0.0034 0.0409 0.003 0.0062 0.0112 0.0015 0.0018 0.0005 0.0049 0.0012 0.0035 0.0001 0.0002
Bud 0.0002 0.0004 0.0005 0.0031 0.0001 0.0018 0.0054 0.0006 0.0054 0.0004 0.0013 0.0003 0.0001 0.0027 0.0031 0.0002 0 0
Bud Neck 0.0015 0.0002 0.0008 0.0146 0.0001 0.001 0.0021 0.0002 0.0002 0.0011 0.0002 0.0083 0.0001 0.0002 0.0002 0.0003 0.0001 0.0001
Bud Periphery 0.0003 0.0002 0.0005 0.0099 0 0.0011 0.0011 0.0007 0.0055 0.0005 0.0009 0.0004 0.0001 0.0029 0.002 0.0002 0 0
Bud Site 0.0006 0.0039 0.0013 0.0019 0 0.0002 0.0178 0.0012 0.0031 0.0004 0.0004 0.0001 0.001 0.0062 0.0137 0.0032 0 0
Cell Periphery 0.0001 0.0001 0.0001 0.0003 0 0.0001 0.0006 0.0002 0.0002 0.0002 0.0001 0.0001 0.0002 0.0004 0.0002 0.0002 0 0
Cytoplasm 0.4442 0.6306 0.5871 0.5957 0.6309 0.6334 0.2752 0.4689 0.4037 0.5164 0.3683 0.4357 0.3765 0.3741 0.4168 0.4444 0.6025 0.5041
Cytoplasmic Foci 0.0204 0.0082 0.0094 0.013 0.0115 0.0074 0.0122 0.0052 0.0089 0.0101 0.0102 0.0065 0.0065 0.0073 0.006 0.0111 0.006 0.0078
Eisosomes 0.0002 0 0.0001 0.0001 0 0 0.0004 0.0001 0.0003 0.0001 0.0001 0 0.0001 0.0002 0.0001 0.0001 0 0
Endoplasmic Reticulum 0.0057 0.002 0.0026 0.0051 0.0019 0.002 0.0028 0.002 0.0018 0.01 0.0011 0.0013 0.0051 0.0028 0.0019 0.0028 0.0019 0.0019
Endosome 0.0086 0.0012 0.004 0.0088 0.003 0.0062 0.0061 0.0026 0.0012 0.0042 0.0007 0.0012 0.0006 0.0005 0.0008 0.0325 0.0022 0.001
Golgi 0.0039 0.0001 0.0021 0.0034 0.0002 0.0002 0.0033 0.0002 0.0006 0.0004 0.0001 0.0002 0.0001 0.0003 0.0002 0.0136 0.0001 0.0001
Lipid Particles 0.0036 0.0003 0.0025 0.0009 0.0005 0.0003 0.0063 0.0003 0.0018 0.0006 0.0004 0.0001 0.0005 0.0005 0.0002 0.011 0.0001 0.0001
Mitochondria 0.0017 0.0002 0.0013 0.0287 0.0004 0.0004 0.0016 0.0002 0.0052 0.0005 0.0005 0.0002 0.0002 0.0004 0.0003 0.0045 0.0001 0.0001
None 0.4776 0.3487 0.3687 0.2833 0.3464 0.3339 0.5868 0.511 0.5452 0.4363 0.6054 0.5412 0.6047 0.593 0.5499 0.4649 0.3841 0.4819
Nuclear Periphery 0.0024 0.0005 0.0011 0.0021 0.0006 0.0037 0.0017 0.0007 0.0007 0.0026 0.0014 0.0003 0.0007 0.0006 0.0005 0.0011 0.0005 0.0004
Nucleolus 0.0003 0.0002 0.0002 0.0003 0 0.0001 0.0012 0.0001 0.0004 0.0001 0.0002 0 0.0002 0.0001 0.0002 0.0002 0 0
Nucleus 0.0013 0.0012 0.0022 0.0046 0.0018 0.0023 0.0024 0.0013 0.0022 0.0021 0.0016 0.001 0.0009 0.0007 0.0008 0.0008 0.001 0.0005
Peroxisomes 0.0013 0.0002 0.004 0.0013 0.0007 0.0002 0.0271 0.0002 0.0049 0.0005 0.0003 0.0004 0.0001 0.0003 0.0002 0.0025 0 0.0002
Punctate Nuclear 0.0041 0.0009 0.002 0.0019 0.0007 0.0013 0.0017 0.0006 0.0017 0.0012 0.0047 0.0007 0.0012 0.0012 0.0011 0.001 0.0002 0.0014
Vacuole 0.0007 0.0007 0.0009 0.0035 0.0006 0.0007 0.0029 0.0006 0.0005 0.0011 0.0004 0.0003 0.0006 0.0005 0.0005 0.0014 0.001 0.0002
Vacuole Periphery 0.0003 0.0001 0.0002 0.0006 0.0001 0.0003 0.0003 0.0001 0.0001 0.0002 0.0001 0 0.0001 0.0001 0.0001 0.0006 0.0001 0

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 3.0172 3.5064 3.8028 2.8598 4.1076 3.3171 3.6817 4.0494 4.0354 4.9086
Translational Efficiency – – – – – – – – – –

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency –

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
263 1387 1203 1218 1364 1703 188 1979 1627 3090 1391 3197

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 822.99 800.94 842.16 872.99 595.65 784.66 931.02 825.51 632.40 791.97 854.17 843.60
Standard Deviation 100.70 106.06 137.67 128.63 79.05 103.53 119.86 108.59 117.82 104.98 138.77 118.89
Intensity Change Log 2 — -0.039181 0.033219 0.085090 — 0.397603 0.644347 0.470821 — 0.160520 0.321830 0.259753

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000597 0.000337 0.000287 0.001442 0.000259 0.000319 0.000250 0.000158 0.000313 0.000327 0.000282 0.000647
Bud Neck 0.007283 0.016276 0.003053 0.007960 0.027049 0.034939 0.001126 0.007862 0.023854 0.026562 0.002792 0.007899
Bud Site 0.035049 0.014333 0.009204 0.066839 0.015509 0.016974 0.002212 0.022241 0.018667 0.015789 0.008259 0.039232
Cell Periphery 0.000232 0.000137 0.000168 0.000214 0.000379 0.000149 0.000164 0.000119 0.000355 0.000143 0.000168 0.000155
Cytoplasm 0.436688* 0.562638* 0.462671* 0.500473* 0.480879* 0.478355* 0.258377* 0.605786* 0.473736* 0.516187* 0.435059* 0.565664*
Cytoplasmic Foci 0.057410 0.186400* 0.013982 0.018857 0.250873* 0.208881* 0.012476 0.012738 0.219600* 0.198790* 0.013779 0.015069
Eisosomes 0.000094 0.000089 0.000036 0.000039 0.000490 0.000147 0.000087 0.000024 0.000426 0.000121 0.000043 0.000030
Endoplasmic Reticulum 0.001305 0.000788 0.002672 0.001282 0.001412 0.000542 0.004926 0.000957 0.001394 0.000652 0.002977 0.001081
Endosome 0.011001 0.015866 0.002134 0.007747 0.008022 0.021678 0.003647 0.001393 0.008504 0.019069 0.002338 0.003814
Golgi 0.016104 0.003941 0.000112 0.012644 0.005747 0.005046 0.000068 0.000174 0.007421 0.004550 0.000106 0.004925
Lipid Particles 0.001408 0.003259 0.001389 0.000749 0.013729 0.005858 0.002750 0.000665 0.011737 0.004692 0.001573 0.000697
Mitochondria 0.013231 0.005050 0.000403 0.004848 0.021654 0.009101 0.000268 0.001337 0.020292 0.007283 0.000384 0.002675
Mitotic Spindle 0.007626 0.004543 0.003941 0.055677 0.001914 0.005548 0.000150 0.017016 0.002837 0.005097 0.003428 0.031746
None 0.001194 0.003475 0.009883 0.008268 0.008415 0.003215 0.004073 0.005684 0.007247 0.003332 0.009098 0.006668
Nuclear Periphery 0.000598 0.000224 0.001098 0.001069 0.000296 0.000269 0.001898 0.000956 0.000345 0.000249 0.001206 0.000999
Nuclear Periphery Foci 0.000898 0.000884 0.001550 0.001327 0.000375 0.000220 0.002218 0.000502 0.000460 0.000518 0.001640 0.000816
Nucleolus 0.000243 0.001021 0.000641 0.001225 0.002375 0.001458 0.000856 0.000350 0.002030 0.001262 0.000670 0.000683
Nucleus 0.162456* 0.097204 0.438895* 0.150787* 0.121453 0.106232 0.581341* 0.258312* 0.128081 0.102180 0.458147* 0.217347*
Peroxisomes 0.001724 0.006050 0.000390 0.003410 0.009240 0.008491 0.000272 0.000276 0.008025 0.007395 0.000374 0.001470
Vacuole 0.238811* 0.076164 0.047285 0.150601 0.027851 0.090533 0.122674 0.062971 0.061952 0.084084 0.057474 0.096357
Vacuole Periphery 0.006047 0.001322 0.000206 0.004539 0.002082 0.002045 0.000166 0.000480 0.002723 0.001720 0.000201 0.002026

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 0.37 0.06 -1.19 -1.46 -1.32 -0.68 -2.52 1.75 1.85 5.47 -0.81 -1.94 -0.76 -0.12 0.48
Bud Neck -4.93 0.66 -0.52 7.15 -3.05 -2.69 13.03 9.49 12.16 -7.92 -1.41 11.67 9.62 13.81 -5.25
Bud Site 1.12 2.15 -1.06 -3.65 -5.08 -0.58 6.83 0.25 0.89 -7.30 0.30 3.71 -1.27 -1.93 -5.37
Cell Periphery 0.33 -0.64 0.78 0.65 2.39 10.33 7.77 12.60 4.57 5.19 9.82 7.44 12.18 3.36 4.15
Cytoplasm -8.90 -1.22 -0.16 15.14 1.78 0.35 13.18 -3.88 -4.22 -15.32 -5.81 7.68 -0.40 6.26 -8.81
Cytoplasmic Foci -19.95 5.01 5.31 33.61 1.27 5.61 42.56 43.44 38.85 0.15 2.93 39.93 40.47 51.28 1.36
Eisosomes -0.73 3.08 2.67 7.67 -1.90 5.97 6.59 8.10 11.68 9.43 6.31 7.75 8.09 13.90 6.12
Endoplasmic Reticulum 3.71 -7.43 -2.03 -10.86 7.54 4.41 -8.31 0.44 -9.45 8.70 4.70 -11.00 -1.06 -13.83 12.56
Endosome -6.12 2.86 2.24 11.76 -1.79 -10.47 5.35 13.28 16.69 6.70 -12.23 10.53 11.82 20.61 3.05
Golgi 0.65 1.57 0.83 0.65 -2.52 0.95 11.21 11.12 7.71 -1.25 1.57 7.40 5.22 5.26 -2.54
Lipid Particles -5.56 -0.84 1.10 7.43 2.92 7.55 10.86 14.11 10.00 8.21 8.44 12.63 13.90 12.23 6.79
Mitochondria 0.12 1.26 0.94 2.59 -3.83 4.98 9.55 9.18 6.65 -6.54 5.49 9.38 8.90 6.81 -6.15
Mitotic Spindle 0.73 1.02 -5.30 -7.45 -7.81 -2.55 3.03 -6.63 -4.11 -7.90 -2.12 -0.43 -9.82 -8.19 -9.25
None -3.59 -6.04 -7.81 -3.33 1.67 4.20 4.77 2.80 -3.01 -4.36 3.97 0.02 1.84 -4.45 1.80
Nuclear Periphery 9.32 -5.93 -11.83 -20.64 -7.47 0.70 -15.71 -21.45 -23.53 5.95 4.68 -18.37 -25.61 -30.91 -3.64
Nuclear Periphery Foci 0.61 -3.75 -1.67 -3.18 2.53 0.92 -7.28 -1.35 -6.17 7.17 -0.34 -8.66 -3.00 -4.89 7.84
Nucleolus -6.80 -5.53 -1.69 1.58 -0.15 1.67 3.40 4.01 5.15 1.59 1.70 3.41 3.63 5.10 1.04
Nucleus 5.47 -17.54 -3.04 -18.12 23.31 2.85 -16.66 -27.61 -31.38 8.66 5.90 -38.68 -24.82 -36.18 22.16
Peroxisomes -7.42 1.52 0.18 7.90 -1.47 0.73 10.46 10.54 13.16 1.44 0.69 10.35 10.10 15.52 -0.71
Vacuole 14.01 14.21 2.44 -23.27 -23.22 -17.36 -10.69 -20.93 -4.91 5.42 -1.99 -2.84 -17.39 -19.59 -14.68
Vacuole Periphery 0.58 1.11 0.62 0.24 -2.38 0.19 11.11 9.23 6.49 -1.88 1.14 5.37 3.77 4.55 -2.71
Standard name
Human Ortholog
Description Putative transporter of the MRP subfamily; adjacent ORFs YKR103W and YKR104W are merged in different strain backgrounds; MRP stands for multidrug resistance-associated protein
Localization
Cell Percentages cytoplasm (38%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Nft1

Nft1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Nft1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available